Amplification consumable receiving bracket and PCR (Polymerase Chain Reaction) amplification equipment

Abstract

The utility model discloses an amplification consumable receiving support and PCR amplification equipment, and relates to the technical field of medical instruments, the amplification consumable receiving support comprises a support base body, a consumable supporting frame capable of being elastically separated from the support base body is arranged in a partial area of the support base body, the consumable supporting frame comprises a plurality of consumable receiving holes, and the consumable receiving holes are communicated with the consumable receiving holes. The amplified consumable receiving support completing receiving can be driven by external force from the bottom or the top, then the consumable bearing frame and the support base body are separated by a preset distance, and the consumable bearing frame and the support base body can be recombined through the elastic effect after no external force exists. According to the amplification consumable receiving bracket and the PCR amplification equipment, the problem that a high-precision motion driving mechanism and a high-precision transmission structure are needed due to the fact that a heat cover and / or a heat cycle module in the prior art need to be integrally driven can be effectively solved; the module weight and the possibility that the reliability of the whole machine becomes poor due to multiple times of warehouse entry and exit need to be considered.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, and particularly relates to an amplification consumable receiving bracket and a PCR amplification device. Background Art

[0002] The variation of nucleic acid sequences (including DNA and RNA) plays an important role in many diseases. Detecting nucleic acid sequence variation at base pair resolution is important for diagnosis and treatment. In an amplification reaction system constructed with polymerase, primers, reaction buffer, and deoxynucleotides (dNTPs), by performing multiple rounds of cyclic amplification on this system, the target fragment can be replicated into thousands, tens of thousands, hundreds of thousands, millions, or even hundreds of millions of molecular copies. Thus, combined with the currently relatively mature electrophoresis method or fluorescence labeling detection method, it is relatively easy to distinguish whether the object to be detected contains the substance to be detected. Of course, in some cases, the concentration of the target sequence can also be accurately quantified. This detection technology is very important in today's world with a high degree of globalization and is necessary for timely control of small-scale epidemic diseases discovered in local areas. At the same time, it is of great significance in application scenarios such as disease diagnosis and treatment, criminalistics, and seed cultivation optimization.

[0003] Currently, large internationally influential companies such as Roche, Abbott, Siemens, and Beckman all have product layouts with nucleic acid sequences as the detection targets. The existing amplification equipment comes in various forms. In actual applications, the number of samples simultaneously detected by different testing institutions varies greatly. Most of the current detection systems are designed with a fixed sample throughput. US20160339437A1 discloses a PCR device that can improve the heat transfer efficiency of the thermal cycling block, which is set with a fixed sample processing capacity. For example, it can include a fixed-type thermal cycling module with 48 or 96 wells. Such a device with a fixed processing capacity may be inapplicable in some detection scenarios. For example, in some detection scenarios, the sample volume changes greatly, and currently, few detection devices that can adapt to different detection volume changes have been developed. In the device with a fixed detection throughput, CN108463288A discloses a solution where the thermal cover can move relative to the sample thermal cycling module and a pressing structure is set so that when the thermal cover approaches the thermal cycling module, it can also press down on the thermal cycling module, thus enabling reliable thermal cycling heating in the subsequent process. However, the moving part of this system is the thermal cover, and there will be a problem of poor system reliability after long-term use. For the top collection scheme, the movement of the thermal cover has a greater impact on the accuracy of fluorescence collection. In another type of device, such as the device disclosed in CN219279840U, a scheme of being able to drive the thermal cycling module and the sample block as a whole is adopted. After the whole is taken out of the chamber, amplification consumables are added, and then the whole is driven back and pressed to perform cyclic amplification. This scheme can ensure the constant temperature control characteristics of the thermal cycling module, and requires special high-precision designs to ensure high precision in the movement in and out of the chamber and less wear of the guide rail after multiple uses and other optimization schemes. However, in the above-mentioned designs, the entire temperature cycling module receives the amplification consumables. On the one hand, the manual loading state needs to be strictly controlled. On the other hand, there are relatively more moving parts, and additional consideration of high-precision relative motion drive is required in high-precision amplification detection.

[0004] Therefore, it is necessary to design a simple and convenient amplification consumable receiving rack, which is independently set from the thermal cover and / or the thermal cycling module. When adding or unloading amplification consumables, it is separately driven out of the device, so as to ensure that the system has higher reliability, and the requirements for the driving structure and precision are also lower. Summary of the Utility Model

[0005] The purpose of the present utility model is: aiming at the above-mentioned existing problems, the present utility model provides an amplification consumable receiving bracket and a PCR amplification device. Currently, the thermal cover and / or the thermal cycling module need to be driven as a whole to achieve relative movement and then add or unload amplification consumables. In this way, a high-precision motion driving mechanism and a high-precision transmission structure need to be set. At the same time, it is also necessary to consider the weight of the module and the possibility of poor overall machine reliability caused by too many in-and-out movements of the chamber. Therefore, it is necessary and urgent to design a solution different from the existing overall out-of-chamber scheme to make the device design simple and the operation convenient, and the driving precision can be relatively low.

[0006] The technical solution adopted by the present utility model is as follows:

[0007] An amplification consumable receiving bracket, comprising a bracket base body, and a consumable supporting bracket which is elastically separable from the bracket base body is arranged in a partial area of the bracket base body; the consumable supporting bracket comprises a plurality of consumable receiving holes arranged in an array; the amplification consumable receiving bracket after receiving can be driven from the bottom or the top by an external force, so that a separation with a preset distance is generated between the consumable supporting bracket and the bracket base body, and after the external force acts, the consumable supporting bracket and the bracket base body can be re-combined by relying on the elastic action.

[0008] Further, a transmission rack is arranged on one side in the width direction of the bracket base body.

[0009] Further, at least one fitting chute is arranged on at least one side in the width direction of the bracket base body.

[0010] Further, the consumable supporting bracket is fixedly connected with at least one guiding column; the other end of the guiding column opposite to the fixed end of the consumable supporting bracket comprises a stop block; the bracket base body comprises through parts corresponding to the number of the guiding columns; at least one guiding column can pass through the through parts, and an elastic element sleeved on the guiding column is arranged between the stop block and the bracket base body.

[0011] Further, a clamping piece for abutting against the elastic element is further arranged between the elastic element and the bracket base body.

[0012] A PCR amplification device, comprising a thermal cycling temperature block capable of being driven to move up and down, and a thermal cover fixed in the PCR amplification device; an above-mentioned amplification consumable receiving bracket capable of being driven to stretch is arranged between the thermal cycling temperature block and the thermal cover, wherein the amplification consumable receiving bracket can be driven to extend to complete the addition or unloading operation; then the amplification consumable receiving bracket can be driven to retract, and the thermal cycling temperature block can rise to contact and apply a driving force to the amplification consumable receiving bracket from the bottom, so that a separation with a preset distance is generated between the consumable supporting bracket and the bracket base body, and further the amplification consumable cover can abut against the bottom of the thermal cover.

[0013] Further, the thermal cover comprises a plurality of guiding frames which are detachably connected, and a plurality of fitting blocks are arranged at intervals on each guiding frame; at least one fitting chute is arranged on at least one side in the width direction of the bracket base body; at least part of the fitting blocks of two adjacent guiding frames can be fittingly connected in the fitting chute.

[0014] Further, a transmission rack is provided on one side in the width direction of the bracket base; at least some of the several guide frames are fixedly connected with a telescopic driving mechanism, and the telescopic driving mechanism includes a telescopic driving motor, the output shaft of which is fixedly connected with a telescopic driving gear; the telescopic driving gear is in meshing transmission connection with the transmission rack.

[0015] Further, at least some of the guide frames are provided with limit blocks, and a baffle is connected to the amplification consumable receiving bracket, and the amplification consumable receiving bracket can be restricted to perform telescopic movement within a stroke range smaller than its length.

[0016] Further, the number of the guide frames is four, and each two adjacent guide frames can be connected with an amplification consumable receiving bracket in a fitting manner to form three amplification consumable receiving brackets that can be independently driven to perform telescopic movement.

[0017] The beneficial effects of the utility model are as follows:

[0018] 1. By designing an amplification consumable receiving bracket that can be independently driven to perform telescopic movement, the utility model includes a bracket base and a consumable supporting bracket that can be elastically separated from it. After the amplification consumables are loaded, it can be driven by an external force to separate the consumable supporting bracket from the bracket base by a preset distance, ensuring that the amplification consumables can be tightly pressed against the hot cover to reliably perform amplification.

[0019] 2. A transmission rack is arranged on one side in the width direction of the bracket base, and is in meshing transmission connection with the telescopic driving gear of the output shaft of the driving motor to ensure that the bracket can be driven to perform telescopic movement with high precision; at least one side in the width direction of the bracket base is provided with a fitting chute, which can be in fitting connection with the fitting block in the guide frame connected to the device, so that the overall driving resistance is small and the output power of the driving motor is lower.

[0020] 3. The consumable supporting bracket is fixedly connected with at least one guide post, and the other end of the guide post opposite to the fixed end of the consumable supporting bracket includes a stop block. The bracket base includes through parts corresponding to the number of the guide posts. At least one guide post can pass through the through parts, and an elastic element sleeved on the guide post is arranged between the stop block and the bracket base. Through this design, the goal of being able to be elastically separated by applying an external force and restored to combination by removing the external force can be simply and reliably achieved, and a clamping piece is arranged to enable the elastic element to be more reliably constrained.

[0021] 4. The utility model also discloses a PCR amplification device, which includes a thermal cycling temperature block that can be driven to move up and down, a hot cover fixed in the PCR amplification device, and an amplification consumable receiving bracket that can be driven to perform telescopic movement is arranged between the thermal cycling temperature block and the hot cover. In this way, the relative moving parts of the whole amplification device are less, and the driving of the bracket movement is simple and efficient.

[0022] 5. Through multiple guide frames and several amplification consumable receiving brackets that are fitted and matched with them, efficient restraint of the amplification consumable receiving brackets can be achieved, independent driving of several amplification consumable receiving brackets can be realized, and the device can be flexibly set to adapt to different detection volume scenarios. Description of the Drawings

[0023] Figure 1 It is a schematic diagram of the state of the amplification consumable receiving bracket of the present utility model without external force;

[0024] Figure 2 It is a schematic diagram of the state of the amplification consumable receiving bracket of the present utility model with external force;

[0025] Figure 3 It is a structural diagram of the bottom direction of the amplification consumable receiving bracket of the present utility model;

[0026] Figure 4 It is a structural diagram of another direction of the bottom of the amplification consumable receiving bracket of the present utility model;

[0027] Figure 5 It is a structural diagram of the guide frame of the present utility model;

[0028] Figure 6 It is a driving state diagram after the thermal cover and the amplification consumable receiving bracket of the present utility model are assembled;

[0029] Figure 7 It is another driving state diagram of the assembly of the thermal cover and the amplification consumable receiving bracket of the present utility model;

[0030] Figure 8 It is a structural diagram of the PCR device of the present utility model;

[0031] Figure 9 It is a schematic diagram of the top view and the sectional position of the PCR device of the present utility model;

[0032] Figure 10 It is a schematic diagram of the first stage of the process of pressing the amplification consumables to the thermal cover after the PCR device of the present utility model loads the amplification consumables;

[0033] Figure 11 It is a schematic diagram of the second stage of the process of pressing the amplification consumables to the thermal cover after the PCR device of the present utility model loads the amplification consumables;

[0034] Figure 12 It is a schematic diagram of the third stage of the process of pressing the amplification consumables to the thermal cover after the PCR device of the present utility model loads the amplification consumables;

[0035] In the attached drawings: 10 - bracket base; 11 - consumable carrier; 111 - consumable receiving hole; 12 - guide post; 120 - elastic element; 121 - stop block; 122 - clamping piece; 13 - transmission rack; 141 - first fitting chute; 142 - second fitting chute; 15 - retaining piece; 20 - guide frame; 21 - connection hole; 211 - fitting block; 200 - heat cover; 221 - telescopic drive motor; 222 - telescopic drive gear; 23 - limit block; 24 - position sensor; 30 - fluorescence detection module; 31 - lateral drive motor; 32 - lateral transmission mechanism; 33 - longitudinal transmission mechanism; 300 - single-tube consumable; 41 - thermal cycling temperature block; 411 - heat dissipation fin. Detailed implementation mode

[0036] The following will combine the attached drawings to make a detailed description of the present utility model.

[0037] In order to make the purpose, technical solution and advantages of the present utility model clearer, the following will further describe the present utility model in detail in combination with the attached drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0038] Embodiment 1

[0039] An amplification consumable receiving bracket, as Figures 1-4 shown, includes a bracket base 10. Here, the bracket base 10 can be set as a basically rectangular structure. In a partial area of the bracket base 10, a consumable carrier 11 that can be elastically separated from the bracket base 10 is provided. Here, in order to ensure the convenience of processing and low weight of the amplification consumable receiving bracket, it can be selected as a plastic material and manufactured by processes such as injection molding. The part of the bracket base 10 where the consumable carrier 11 is not provided is a constraint part, which can be at least partially constrained in the amplification device to ensure that the consumable carrier 11 exposed outside the device can reliably receive the amplification consumables. The consumable carrier 11 includes a plurality of consumable receiving holes 111 arranged in an array. Here, the consumable receiving holes 111 can be set as an 8*4 matrix, and of course, other quantities can also be set. The plurality of consumable receiving holes 111 can engage with specific positions on the upper half of the amplification consumables to achieve the effect of reliably receiving the amplification consumables. The amplification consumables can also be set as a corresponding whole-board array type, or can be set as a single-row multi-tube type, such as an 8-tube consumable. More generally, it can also be an independent single-tube consumable 300. After the amplification consumable receiving bracket that has completed receiving is driven to retract into the device, then in order to perform amplification, the thermal cycling temperature block 41 can apply an external force from the bottom or the heat cover 200 can apply an external force from the top, and then drive the amplification consumable receiving bracket from the bottom or the top, so that a preset distance separation is generated between the consumable carrier 11 and the bracket base 10 as Figure 2As shown, in order to ensure the reliability of the rebound combination between the two and the tightness of the fit between the amplification consumables and the bottom thermal cycling temperature block 41 and the top thermal cover 200, the preset separation distance range is between 8 mm and 15 mm. An excessive distance will cause the excessive fit force between the amplification consumables and the thermal cover 200, which may cause the consumables to deform or be damaged and unable to be accurately detected. While a too small distance cannot ensure the fit degree between the amplification consumables and the thermal cover 200, which may cause insufficient heat transfer between the thermal cover 200 and the amplification consumables cover, resulting in volume loss of the amplification reaction system. Seriously, it will lead to inaccurate test results. After the external force is removed, for example, after the amplification is completed, the thermal cycling temperature block 41 or the thermal cover 200 moves relatively away. In this way, the amplification consumables receiving bracket can rely on the elastic action to re-combine the consumable support bracket 11 and the bracket base 10 as Figure 1 shown, and can be driven to extend to take out the amplification consumables. Combining Figure 3 and Figure 4 , a transmission rack 13 is provided on one side in the width direction of the bracket base 10. Optimally, each amplification consumables receiving bracket only includes 1 transmission rack 13, so as to avoid conflicts between different transmission structures and ensure that the bracket can be accurately driven for telescopic movement. At least one side in the width direction of the bracket base 10 is provided with a fitting chute. Here, in order to ensure that the amplification consumables bracket is reliably clamped and the telescopic movement can be stable without tilting deformation, fitting chutes are provided on both sides in the width direction, namely the first fitting chute 141 and the second fitting chute 142 respectively. In this embodiment, in order to ensure the elastic separation characteristic between the bracket base 10 and the consumable support bracket 11, the consumable support bracket 11 is fixedly connected with at least one guide post 12. In the attached drawing, one end of 4 guide posts 12 is fixedly connected to the consumable support bracket 11. Here, the fixed connection can be a detachable fixed connection. The other end of the guide post 12 opposite to the fixed end of the consumable support bracket 11 includes a stop block 121. The bracket base 10 includes through parts corresponding to the number of guide posts 12. At least one guide post 12 can pass through the through parts, and an elastic element 120 sleeved on the guide post 12 is arranged between the stop block 121 and the bracket base 10. Here, the elastic element 120 is a threaded spring, and its stiffness coefficient range is 0.3 N / mm - 0.46 N / mm. In this way, it can ensure that the elastic force generated by the spring is more suitable for this bracket setting, and can also prevent the elastic action from being too large to cause consumable deformation or too small to cause poor heat transfer characteristics. Of course, in order to ensure that the elastic element 120 is reliably clamped, a clamping piece 122 abutting against the elastic element 120 is also arranged between the elastic element 120 and the bracket base 10. It can be integrally or separately designed with the bracket base 10, and can also strengthen the strength characteristics of the contact part between the elastic element 120 and the bracket base 10.

[0040] Embodiment 2

[0041] A PCR amplification device, the structure and part of the operation process can be referred to Figures 5-12, overall, it includes several important modules. The thermal cover 200 located at the upper part is used to apply a certain pressing force to the top of the amplification consumable cover during the amplification process, and can heat the amplification consumable cover to keep it in a hot state, thereby reducing the problem that the amplification solution evaporates and condenses on the consumable cover, resulting in a decrease in the amplification solution and the inability to collect and detect from the top due to condensation. Figure 6 and Figure 7 shows a schematic structural diagram of a thermal cover 200. Several spaced guide frames 20 are fixedly connected to the bottom of the thermal cover 200. Combining Figure 5The guide frame 20 can be detachably connected to the bottom of the heat cover 200 through the connecting hole 21. In order to ensure that the amplification consumables receiving bracket and the guide frame 20 can be slidably connected with low resistance, a plurality of interlocking blocks 211 are arranged at intervals on each guide frame 20. Here, the amplification consumables receiving bracket is guided and constrained by a plurality of interlocking blocks 211 rather than a continuous guide rail, which has lower resistance and less wear during long-term operation, thereby improving the reliability of the equipment. At least part of the interlocking blocks 211 of two adjacent guide racks 20 can be interlockedly connected to the interlocking grooves in the width direction of the bracket base 10. In this embodiment, in order to ensure that the device can run multiple groups of consumables to be amplified in parallel, four guide racks 20 are arranged at equal intervals at the bottom of the heat cover 200. Every two adjacent guide racks 20 can be interlockedly connected to an amplification consumable receiving rack to form three amplification consumable receiving racks that can be driven to independently telescopically move. The consumable support bracket 11 of each amplification consumable receiving rack includes thirty-two amplification consumable receiving holes 111, so that the amplification processing volume can be adjusted variably. During the amplification cycle in some amplification consumable receiving racks, amplification consumables can be added to other amplification consumable receiving racks, ensuring that the device operation is more flexible. Of course, different amplification consumables can also receive samples to be amplified that execute different target temperatures to achieve an effect similar to gradient PCR, and can also ensure that the amplification cycles in different racks in this scenario have almost no mutual influence. At least part of the several guide frames 20 are fixedly connected with a telescopic driving mechanism, where each telescopic driving mechanism is arranged on the same side of the amplification consumables receiving bracket and the two correspond in number. The telescopic driving mechanism includes a telescopic driving motor 221, which can be detachably fixedly connected to the guide frame 20, and the output shaft is fixedly connected to the telescopic driving gear 222; the telescopic driving gear 222 is meshed and transmission-connected with a transmission rack 13 arranged on one side in the width direction of the bracket base 10, so that when the telescopic driving motor 221 rotates clockwise or counterclockwise, the amplification consumables receiving bracket can be driven to extend or retract the device to perform different operations. At least part of the guide frame 20 is provided with a limit block 23, and the amplification consumables receiving bracket is connected to a baffle 15. When the amplification consumables receiving bracket is driven to extend, the fit between the two can limit the telescopic movement of the amplification consumables receiving bracket to a stroke range less than its length. In order to ensure the stability of the amplification consumables receiving bracket, the length of the amplification consumables receiving bracket extending out of the range of the equipment shall not exceed 2 / 3 of the total length of the bracket. In order to accurately know the position of the amplification consumables receiving bracket, at least one position sensor 24 is also connected to the bottom of the heat cover 200.

[0042] Figure 8 and Figure 9Schematically shows the overall structure diagram of a PCR amplification device. A fluorescence detection module 30 is provided on the top of the thermal cover 200. It can be driven to move horizontally by a horizontal drive motor 31-driven horizontal transmission mechanism 32 and can also be driven to move vertically by a vertical transmission mechanism 33. A plurality of through holes are arranged in an array on the thermal cover 200, so that the fluorescence detection module 30 can be driven to scan in the horizontal plane to complete the detection. In the lower region, there is a thermal cycling temperature block 41 that can be driven to move up and down. The bottom includes heat dissipation fins 411 and an air duct wrapping it, so that each thermal cycling temperature block 41 can perform independent heating and cooling operations. An amplification consumable receiving bracket that can be driven to move telescopically is provided between the thermal cycling temperature block 41 and the thermal cover 200. The thermal cover 200 and the amplification consumable receiving bracket can be connected in the manner described previously. When adding or unloading amplification consumables, the amplification consumable receiving bracket can be driven to extend to complete the addition or unloading operation. Under the restraint of the limit block 23 and the stop piece 15, only a part of the amplification consumable receiving bracket extends out of the device. When performing cyclic amplification, the amplification consumable receiving bracket can be driven to retract, and the thermal cycling temperature block 41 can rise to contact and apply a driving force to the amplification consumable receiving bracket from the bottom.

[0043] The process of the thermal cycling temperature block 41 rising and pressing the amplification consumables can be understood in combination with the AA-sectional view of the PCR device. The process is as Figures 10-12 shown. The amplification consumable receiving bracket after receiving the amplification consumables can be driven to retract into the device. Here, it is schematically shown that three independent amplification consumable receiving brackets are all provided with a sufficient amount of amplification consumables. In other states, only some of the amplification consumable brackets can be provided with amplification consumables or a sufficient amount of amplification consumables can be set. The thermal cycling temperature block 41 at the bottom of the amplification consumable receiving bracket provided with amplification consumables is driven to rise. Figures 10-11 Schematically shows this process. During the rising process, the thermal cycling temperature block 41 can apply a driving force to the amplification consumable receiving bracket from below. Here, it can directly or indirectly apply an upward driving force to the consumable support bracket 11 through the side wall of the bottom of the amplification consumable and / or the consumable support bracket 11. And the bracket base 10 of the amplification consumable receiving bracket can be fixed at a specific position when retracting. In this way, as the thermal cycling temperature block 41 rises, the consumable support bracket 11 is separated from the bracket base 10 by a preset distance until the amplification consumable cover can abut against the bottom of the thermal cover 200 and can maintain an appropriate pressing force under the action of the elastic unit. In this way, at least part of the amplification device can reliably and efficiently perform amplification cycles and fluorescence detection. After completion, the opposite movement drive is performed to make the thermal cycling temperature block 41 descend. Finally, the amplification consumable receiving bracket is driven to extend out of the device to perform consumable unloading and / or reload amplification consumables.

[0044] In this article, specific embodiments are used to elaborate on the principles and implementation manners of the present utility model. The description of the above embodiments is only used to help understand the method and its core idea of the present utility model. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present utility model, several improvements and modifications can still be made to the present utility model, and these improvements and modifications also fall within the protection scope of the claims of the present utility model.

[0045] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the utility model product is usually placed during use. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0046] In the description of the present utility model, it should also be noted that unless otherwise clearly specified and defined, the terms "set", "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

Claims

1. An amplification consumables receiving bracket, characterized in that: The invention comprises a support base (10), and a consumable support bracket (11) which can be elastically separated from the support base (10) is arranged in a partial area of ​​the support base (10); the consumable support bracket (11) comprises a plurality of consumable receiving holes (111) arranged in an array; the receiving bracket of the amplification consumables that have been received can be driven from the bottom or the top by an external force, thereby causing the consumable support bracket (11) to be separated from the support base (10) by a preset distance, and after no external force is applied, the consumable support bracket (11) can be re-combined with the support base (10) by means of elastic action.

2. The amplification consumables receiving bracket according to claim 1, characterized in that: A transmission rack (13) is provided on one side of the bracket base (10) in the width direction.

3. The amplification consumables receiving bracket according to claim 1, characterized in that: At least one side of the bracket base (10) in the width direction is provided with an engaging sliding groove.

4. The amplification consumables receiving bracket according to claim 1, characterized in that: The consumable support bracket (11) is fixedly connected to at least one guide column (12); the other end of the guide column (12) opposite to the fixed end of the consumable support bracket (11) includes a stopper (121); the support base (10) includes a number of through-holes corresponding to the number of the guide columns (12); at least one of the guide columns (12) can pass through the through-holes, and an elastic element (120) penetrating the guide column (12) is provided between the stopper (121) and the support base (10).

5. The amplification consumables receiving bracket according to claim 4, characterized in that: A clamping piece (122) abutting against the elastic element (120) is also provided between the elastic element (120) and the bracket base (10).

6. A PCR amplification device, characterized in that: The invention comprises a thermal cycle block (41) which can be driven to move up and down, and a thermal cover (200) fixed in a PCR amplification device; an amplification consumable receiving bracket as claimed in any one of claims 1 to 5 which can be driven to move in a telescopic manner is arranged between the thermal cycle block (41) and the thermal cover (200), wherein the amplification consumable receiving bracket can be driven to extend to complete the adding or unloading operation; and then the amplification consumable receiving bracket can be driven to retract, and the thermal cycle block (41) can rise to contact and apply a driving force to the amplification consumable receiving bracket from the bottom, so that the consumable support bracket (11) and the bracket base (10) are separated by a preset distance, thereby enabling the amplification consumable cover to abut against the bottom of the thermal cover (200).

7. The PCR amplification device according to claim 6, characterized in that: The thermal cover (200) comprises a plurality of detachably connected guide frames (20), each of the guide frames (20) being provided with a plurality of interlocking blocks at intervals; an interlocking slide groove is provided on at least one side in the width direction of the support base (10); and at least part of the interlocking blocks of two adjacent guide frames (20) can be interlockedly connected in the interlocking slide groove.

8. The PCR amplification device according to claim 7, characterized in that: A transmission rack (13) is provided on one side of the bracket base (10) in the width direction; at least part of the plurality of guide frames (20) is fixedly connected to a telescopic drive mechanism; the telescopic drive mechanism comprises a telescopic drive motor (221), an output shaft of which is fixedly connected to a telescopic drive gear (222); the telescopic drive gear (222) is meshed and transmission-connected with the transmission rack (13).

9. The PCR amplification device according to claim 7, characterized in that: At least part of the guide frame (20) is provided with a limit block (23), and the amplification consumables receiving bracket is connected with a baffle (15), so that the amplification consumables receiving bracket can be restricted to telescopic movement within a stroke range smaller than its length.

10. The PCR amplification device according to claim 7, characterized in that: The number of the guide frames (20) is four, and every two adjacent guide frames (20) can be connected in a chimeric connection to an amplification consumable receiving bracket to form three amplification consumable receiving brackets that can be independently driven to move telescopically.

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