Real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection device

Abstract

The utility model discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection device which comprises a detection seat, the upper end of the detection seat is fixedly connected with a top cover through a bolt, the lower end of the top cover is fixedly connected with a silicon photomultiplier tube, a dustproof component is arranged in the detection seat, a plano-convex lens is fixedly connected in the detection seat, and the plano-convex lens is arranged in the detection seat. The interior of the detection seat is fixedly connected with an optical filter, the interior of the detection seat is fixedly connected with a dichroic mirror, and the interior of the detection seat is fixedly connected with a cylindrical lens. According to the utility model, the upper part of the plano-convex lens is sealed, so that impurities are prevented from falling on the plano-convex lens, and when the plano-convex lens is used, the sealing plate is moved away; and when sealing of the sealing plate is relieved, impurities above the sealing plate can fall into a cleaning groove, the cleaning groove can be conveniently cleaned through a cleaning rod, and the situation that the detection work is affected after the impurities fall onto the plano-convex lens is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescence quantitative PCR detection, in particular to a real-time fluorescence quantitative PCR detection device. Background Technique

[0002] Real-time fluorescence quantitative PCR (real-time quantitative PCR, qPCR) is a DNA or RNA quantitative analysis technique widely used in molecular biology and medical research. This technique can quickly, accurately and sensitively detect and quantify specific target sequences in samples. The real-time fluorescence quantitative PCR detection device can perform various detections on samples with high throughput, high efficiency and high sensitivity, such as gene expression analysis, microorganism detection, virus detection, etc. Due to its real-time and quantitative characteristics, it has important application values in the fields of clinical diagnosis, disease monitoring and drug research and development.

[0003] In the prior art, a real-time fluorescence quantitative PCR detection device (publication number: CN219010300U) is provided. The device includes a PCR reaction tube, and a sealing film is provided at the opening of the PCR reaction tube; it also includes a detection seat, an optical detection component for detecting the sample in the PCR reaction tube, and an opening film component for piercing the sealing film; the optical detection component and the opening film component are both arranged on the detection seat. The structure of the utility model is simply arranged. Through the arrangement of the opening film component, the sealing film can be pierced only when it is necessary to detect the sample in the PCR reaction tube, which can effectively prevent the PCR reaction tube from being in an open state all the time, resulting in the sample in the tube being contaminated and affecting subsequent detections.

[0004] However, this device still has the following defects:

[0005] When the device is in use, when the top cover is installed, impurities may enter the interior of the detection seat and fall on the plano-convex lens, affecting the use of the plano-convex lens and interfering with the accuracy of the subsequent detection results. Moreover, it is not convenient to clean the plano-convex lens. Therefore, we need to propose a real-time fluorescence quantitative PCR detection device. Content of the Utility Model

[0006] The purpose of the utility model is to provide a real-time fluorescence quantitative PCR detection device to seal the upper part of the plano-convex lens to prevent impurities from falling on the plano-convex lens. During use, just move the sealing plate away to solve the problems raised in the above background technique.

[0007] To achieve the above purpose, the utility model provides the following technical solutions:

[0008] A real-time fluorescence quantitative PCR detection device, comprising a detection base, the upper end of the detection base is fixedly connected with a top cover through bolts, the lower end of the top cover is fixedly connected with a silicon photomultiplier tube, a dust-proof component is arranged inside the detection base, a plano-convex lens is fixedly connected inside the detection base, a filter is fixedly connected inside the detection base, a dichroic mirror is fixedly connected inside the detection base, a cylindrical lens is fixedly connected inside the detection base, a collimating lens is fixedly connected inside the detection base, the side of the detection base is fixedly connected with a side plate through bolts, an LED lamp is fixedly connected to the outside of the side plate, and a lifting component is arranged outside the detection base.

[0009] Preferably, the dust-proof component includes a cleaning groove, a cleaning groove is arranged inside the detection base, a cleaning rod is slidably connected inside the cleaning groove, a spring is arranged inside the cleaning groove, a sealing plate is hinged inside the detection base, the outside of the sealing plate contacts an installation plate, an electromagnet is fixedly connected inside the detection base, and the electromagnet is magnetically connected to the sealing plate.

[0010] Preferably, the lifting component includes a mounting plate, the mounting plate is fixedly connected to the outside of the detection base, a motor is fixedly connected to the upper end of the mounting plate, a mounting column is fixedly connected to the lower end of the mounting plate, a screw rod is fixedly connected to the output shaft of the motor, and the screw rod is rotatably connected to the mounting column.

[0011] Preferably, a telescopic rod is fixedly connected inside the detection base, and a moving plate is fixedly connected to the output end of the telescopic rod.

[0012] Preferably, the moving plate is slidably connected to the detection base, and a spreading member is fixedly connected to the lower end of the moving plate.

[0013] Preferably, one end of the spring is fixedly connected to the cleaning groove, and the other end of the spring is fixedly connected to the installation plate.

[0014] Preferably, a lifting plate is threadedly connected to the outside of the screw rod, the lifting plate is slidably connected to the mounting column, and a rubber block is fixedly connected to the upper end of the lifting plate.

[0015] Preferably, the outside of the rubber block contacts a reaction tube, and a test tube rack is slidably connected to the outside of the reaction tube.

[0016] Compared with the prior art, the beneficial effects of the present utility model are:

[0017] The utility model seals the upper part of the plano-convex lens to prevent impurities from falling on the plano-convex lens. During use, the sealing plate can be moved away. Moreover, when the sealing is released, the impurities above the sealing plate can fall into the cleaning groove, and the cleaning rod can facilitate the cleaning of the cleaning groove, avoiding the influence on the detection work after impurities fall on the plano-convex lens. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural view of the utility model;

[0019] Figure 2 is a schematic internal structural view of the utility model;

[0020] Figure 3 This is for the Figure 2 enlarged view of area A in;

[0021] Figure 4 is a schematic structural view of the mounting plate and mounting column etc. of the utility model.

[0022] In the figure: 1, detection seat; 2, top cover; 3, silicon photomultiplier tube; 4, dust-proof component; 41, cleaning groove; 42, cleaning rod; 43, spring; 44, mounting disc; 45, sealing plate; 46, electromagnetic block; 5, plano-convex lens; 6, filter; 7, dichroic mirror; 8, cylindrical lens; 9, collimating lens; 10, LED lamp; 11, side plate; 12, telescopic rod; 13, moving plate; 14, expanding member; 15, lifting component; 151, mounting plate; 152, motor; 153, mounting column; 154, screw; 155, lifting plate; 156, rubber block; 16, reaction tube; 17, test tube rack. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all of the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0024] Please refer to Figures 1-4 , the present utility model provides a technical solution:

[0025] A real-time fluorescence quantitative PCR detection device includes a detection base 1. The upper end of the detection base 1 is fixedly connected to a top cover 2 by bolts. The lower end of the top cover 2 is fixedly connected to a silicon photomultiplier tube 3. A dust-proof component 4 is provided inside the detection base 1. A plano-convex lens 5 is fixedly connected inside the detection base 1. A filter 6 is fixedly connected inside the detection base 1. A dichroic mirror 7 is fixedly connected inside the detection base 1. A cylindrical lens 8 is fixedly connected inside the detection base 1. A collimating lens 9 is fixedly connected inside the detection base 1. The side of the detection base 1 is fixedly connected to a side plate 11 by bolts. An LED lamp 10 is fixedly connected to the outside of the side plate 11. A lifting component 15 is provided outside the detection base 1.

[0026] Specifically, the dust-proof component 4 includes a cleaning groove 41. The cleaning groove 41 is provided inside the detection base 1. A cleaning rod 42 is slidably connected inside the cleaning groove 41. A spring 43 is provided inside the cleaning groove 41. A sealing plate 45 is hinged inside the detection base 1. The outside of the sealing plate 45 contacts an installation plate 44. An electromagnet 46 is fixedly connected inside the detection base 1. The electromagnet 46 is magnetically connected to the sealing plate 45.

[0027] In this embodiment, the lifting component 15 includes an installation plate 151. The installation plate 151 is fixedly connected to the outside of the detection base 1. A motor 152 is fixedly connected to the upper end of the installation plate 151. An installation column 153 is fixedly connected to the lower end of the installation plate 151. A screw rod 154 is fixedly connected to the output shaft of the motor 152. The screw rod 154 is rotatably connected to the installation column 153.

[0028] On the other hand, a telescopic rod 12 is fixedly connected inside the detection base 1. A moving plate 13 is fixedly connected to the output end of the telescopic rod 12.

[0029] Through the design of the telescopic rod 12, the telescopic rod 12 drives the moving plate 13 to move up and down to control the position of the opening member 14.

[0030] It is worth mentioning that the moving plate 13 is slidably connected to the detection base 1. An opening member 14 is fixedly connected to the lower end of the moving plate 13.

[0031] Through the design of the opening member 14, the opening member 14 opens the sealing film on the reaction tube 16.

[0032] It should be noted that one end of the spring 43 is fixedly connected to the cleaning groove 41, and the other end of the spring 43 is fixedly connected to the installation plate 44.

[0033] Through the design of the spring 43, the spring 43 controls the position of the installation plate 44 by elasticity, and elastically bounces the installation plate 44 towards the sealing plate 45 to make the two sealing plates 45 fit and seal, and the two sealing plates 45 are made of iron blocks.

[0034] In addition, a lifting plate 155 is threadedly connected to the outer side of the screw rod 154 , the lifting plate 155 is slidably connected to the mounting column 153 , and a rubber block 156 is fixedly connected to the upper end of the lifting plate 155 .

[0035] Through the design of the screw rod 154 , a threaded motion occurs between the screw rod 154 and the lifting plate 155 to control the lifting and lowering of the lifting plate 155 .

[0036] Exemplarily, the outer side of the rubber block 156 contacts the reaction tube 16 , and the outer side of the reaction tube 16 is slidably connected to the test tube rack 17 .

[0037] Through the design of the rubber block 156 , when the lifting plate 155 is moving, the rubber block 156 protects the bottom end of the reaction tube 16 .

[0038] Working principle: When the utility model is used, the telescopic rod 12 drives the moving plate 13 to move downward, and the opening member 14 opens the sealing film on the reaction tube 16. When the opened reaction tube 16 is moved to the bottom of the detection seat 1, the LED lamp 10 of a specific wavelength emits excitation light, and the excitation light is scattered at a certain emission angle to form scattered light. After the scattered light is collimated by the straight lens 9 and the cylindrical lens 8, most of the scattered light is collimated into parallel light. The parallel light passes through the filter 6 of a specific wavelength range, filters out the spectrum outside the required wavelength range, and then enters the dichroic mirror 7. The refracted parallel light passes through the dichroic mirror 7 and the plano-convex lens 5 for light focusing. The focusing point of the parallel light is located below the liquid surface of the sample to be tested in the reaction tube 16. Since the sample to be tested in the reaction tube 16 has fluorescent substances, the fluorescent substances emit other specific wavelength spectra under excitation. After the emitted light is collimated by the dichroic mirror 7 and the plano-convex lens 5, the emitted light is transmitted through the dichroic mirror 7. The transmitted light passes through the filter 6 of a specific wavelength range to filter out the spectrum outside the required wavelength range. The filtered spectrum is focused by the plano-convex lens 5, and the focusing point is the silicon photomultiplier tube 3. The silicon photomultiplier tube 3 transmits the data to the computer for data analysis. After the detection is completed, the reaction tube 16 is taken out by the test tube rack 17, and the motor 152 drives the screw 154 to rotate. Through the threaded movement between the lifting plate 155 and the screw 154, the lifting plate 155 is controlled to raise the reaction tube 16 for easy removal. The rubber block 156 protects the bottom end of the reaction tube 16 during the lifting process.

[0039] When the top cover 2 is installed and removed, the installation plate 44 is elastically bounced toward the sealing plate 45 so that the two sealing plates 45 are fitted and sealed. When the inspection begins, the electromagnetic block 46 is energized to attract the two iron sealing plates 45 to release the seal of the sealing plates 45. When the sealing plates 45 are vertical, impurities above fall into the cleaning groove 41 and are cleaned by moving the cleaning rod 42.

[0040] Although embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A real-time fluorescence quantitative PCR detection device, characterized in that: include: A detection seat (1), the upper end of which is fixedly connected to a top cover (2) via bolts; A silicon photomultiplier tube (3) is fixedly connected to the lower end of the top cover (2); a dustproof component (4) is provided inside the detection seat (1); a plano-convex lens (5) is fixedly connected inside the detection seat (1); a filter (6) is fixedly connected inside the detection seat (1); a color separation mirror (7) is fixedly connected inside the detection seat (1); a cylindrical lens (8) is fixedly connected inside the detection seat (1); and a straight lens (9) is fixedly connected inside the detection seat (1); A side plate (11) is fixed to the side of the detection seat (1) by means of bolts, an LED lamp (10) is fixedly connected to the outer side of the side plate (11), and a lifting assembly (15) is arranged on the outer side of the detection seat (1).

2. A real-time fluorescence quantitative PCR detection device according to claim 1, characterized in that: The dustproof component (4) comprises a cleaning groove (41), the interior of the detection seat (1) is provided with a cleaning groove (41), the interior of the cleaning groove (41) is slidably connected with a cleaning rod (42), the interior of the cleaning groove (41) is provided with a spring (43), the interior of the detection seat (1) is hinged with a sealing plate (45), the outer side of the sealing plate (45) is in contact with a mounting plate (44), the interior of the detection seat (1) is fixedly connected with an electromagnetic block (46), and the electromagnetic block (46) is magnetically connected to the sealing plate (45).

3. A real-time fluorescence quantitative PCR detection device according to claim 1, characterized in that: The lifting assembly (15) comprises a mounting plate (151), the outer side of the detection seat (1) is fixedly connected to the mounting plate (151), the upper end of the mounting plate (151) is fixedly connected to a motor (152), the lower end of the mounting plate (151) is fixedly connected to a mounting column (153), the output shaft of the motor (152) is fixedly connected to a screw rod (154), and the screw rod (154) is rotatably connected to the mounting column (153).

4. A real-time fluorescence quantitative PCR detection device according to claim 1, characterized in that: A telescopic rod (12) is fixedly connected inside the detection seat (1), and a movable plate (13) is fixedly connected to the output end of the telescopic rod (12).

5. A real-time fluorescence quantitative PCR detection device according to claim 4, characterized in that: The movable plate (13) is slidably connected to the detection seat (1), and the lower end of the movable plate (13) is fixedly connected to a support member (14).

6. A real-time fluorescence quantitative PCR detection device according to claim 2, characterized in that: One end of the spring (43) is fixedly connected to the cleaning groove (41), and the other end of the spring (43) is fixedly connected to the mounting plate (44).

7. A real-time fluorescence quantitative PCR detection device according to claim 3, characterized in that: The outer side of the screw rod (154) is connected to a lifting plate (155) via a thread, the lifting plate (155) is slidably connected to the mounting column (153), and the upper end of the lifting plate (155) is fixedly connected to a rubber block (156).

8. A real-time fluorescence quantitative PCR detection device according to claim 7, characterized in that: The outer side of the rubber block (156) contacts a reaction tube (16), and the outer side of the reaction tube (16) is slidably connected to a test tube rack (17).

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