Gene sequencing fluid system and gene sequencer

Abstract

The utility model provides a gene sequencing fluid system and a gene sequencer, in the gene sequencing fluid system, a first liquid outlet / inlet of a flow cell assembly, a pump assembly and a waste liquid storage device are respectively connected with one port of a first selection valve assembly through fluid pipelines; the first selector valve assembly is configured to selectively communicate the pump assembly with one of the first liquid outlet / inlet, the waste liquid storage device and air; a second liquid outlet / inlet of the flow cell assembly and a plurality of reagent holes of the reagent storage device are respectively connected with one port of the second selection valve assembly through fluid pipelines, and the second selection valve assembly is configured to selectively communicate the second liquid outlet / inlet with any one of the reagent holes and air. According to the scheme, the selection effect of the first selection valve assembly and the second selection valve assembly is utilized, a large number of connecting pipelines can be saved, then the pipeline arrangement space can be saved, and the integration level of the gene sequencing fluid system is improved.

Description

Technical Field

[0001] The present application relates to the technical field of gene sequencing, and particularly to a gene sequencing fluid system and a gene sequencer. Background Art

[0002] With the increasing maturity of gene sequencing technology, gene sequencers are also developing towards miniaturization. The miniaturized gene sequencer not only reduces the production cost, but also makes the sequencing equipment more portable and easy to use, so that it can be more widely applied to scenarios such as clinical diagnosis, mobile laboratories, and scientific research work in remote areas.

[0003] The complex fluid system inside the gene sequencer is one of the important obstacles restricting the miniaturization development of gene sequencers. Therefore, a highly integrated fluid system is of great significance for the development of miniaturized gene sequencers. Utility Model Content

[0004] The present application provides a gene sequencing fluid system and a gene sequencer to improve the integration degree of the gene sequencing fluid system.

[0005] In a first aspect, the present application provides a gene sequencing fluid system, which includes: a reagent storage, a flow cell assembly, a pump assembly, a waste liquid storage, a first selection valve assembly, and a second selection valve assembly; both opposite ends of the flow cell assembly respectively have a first liquid inlet / outlet and a second liquid inlet / outlet for fluid to flow in or out; the reagent storage includes a plurality of reagent holes; wherein,

[0006] The first selection valve assembly has a plurality of selection ports and a common port. The first liquid inlet / outlet and the waste liquid storage are respectively connected to a selection port of the first selection valve assembly through fluid pipelines, and the pump assembly is connected to the common port of the first selection valve assembly through a fluid pipeline; the first selection valve assembly is configured to selectively connect the pump assembly with the first liquid inlet / outlet, the waste liquid storage, and air.

[0007] The second selection valve assembly has a plurality of selection ports and a common port. A plurality of the reagent holes are respectively connected to a selection port of the second selection valve assembly through fluid pipelines; the second liquid inlet / outlet is connected to the common port of the first selection valve assembly through a fluid pipeline, and the second selection valve assembly is configured to selectively connect the second liquid inlet / outlet with any one of the plurality of reagent holes and air.

[0008] For the above gene sequencing fluid system, the waste liquid storage and the flow cell assembly can be connected to the pump assembly through pipelines only by connecting them to the first selection valve assembly. At the same time, the second selection valve assembly can selectively connect multiple reagent holes of the reagent storage to the flow cell assembly. By using the selection functions of the first selection valve assembly and the second selection valve assembly, a large number of connecting pipelines can be saved, thereby saving the pipeline layout space and improving the integration of the gene sequencing fluid system.

[0009] In a possible implementation manner, the gene sequencing fluid system further includes a cleaning liquid storage, and the cleaning liquid storage is connected to a selection port of the first selection valve assembly through a fluid pipeline;

[0010] The first selection valve assembly is further configured to selectively connect the cleaning liquid storage to the pump assembly and connect the pump assembly to the first liquid inlet / outlet.

[0011] In a possible implementation manner, the gene sequencing fluid system further includes an airtightness detection device;

[0012] The airtightness detection device is arranged in the fluid pipeline between a selection port of the first selection valve assembly and a selection port of the second selection valve assembly;

[0013] The airtightness detection device is configured to detect the airtightness of the loop where it is located.

[0014] In a possible implementation manner, the gene sequencing fluid system further includes a bubble sensor, and the bubble sensor is arranged in the fluid pipeline between the waste liquid storage and a selection port of the first selection valve assembly;

[0015] The bubble sensor is configured to detect the number of bubbles in the fluid to be discharged into the waste liquid storage.

[0016] In a possible implementation manner, the first selection valve assembly and the pump assembly are integrated into one body.

[0017] In a possible implementation manner, the flow cell assembly includes an even number of flow channels greater than one; opposite ends of each flow channel have a first end hole and a second end hole for fluid to enter and exit;

[0018] The first end holes of every two flow channels are connected to one of the first liquid inlets / outlets through a manifold pipeline, and the second end holes of every two flow channels are connected to one of the second liquid inlets / outlets through a manifold pipeline;

[0019] One of the first liquid in / out ports is connected to a selection port of the first selection valve assembly, and one of the second liquid in / out ports is connected to the common port of one of the second selection valve assemblies.

[0020] In a possible implementation, the flow cell assembly includes an even number of flow channels greater than one; opposite ends of each flow channel have a first end hole and a second end hole for fluid to flow in and out;

[0021] The first end hole of each flow channel is connected to one of the first liquid in / out ports through a fluid pipeline, and the second end hole of each flow channel is connected to one of the second liquid in / out ports through a fluid pipeline;

[0022] One of the first liquid in / out ports is connected to a selection port of the first selection valve assembly, and one of the second liquid in / out ports is connected to the common port of one of the second selection valve assemblies.

[0023] In a possible implementation, the flow cell assembly includes an even number of flow channels greater than one; opposite ends of each flow channel have a first end hole and a second end hole for fluid to flow in and out;

[0024] The first end hole of each flow channel is connected to one of the first liquid in / out ports through a fluid pipeline, and the second end holes of every two flow channels are connected to one of the second liquid in / out ports through a manifold pipeline;

[0025] One of the first liquid in / out ports is connected to a selection port of the first selection valve assembly, and one of the second liquid in / out ports is connected to the common port of one of the second selection valve assemblies.

[0026] In a possible implementation, the flow cell assembly includes an even number of flow channels greater than one; opposite ends of each flow channel have a first end hole and a second end hole for fluid to flow in and out;

[0027] The first end holes of every two flow channels are connected to one of the first liquid in / out ports through a manifold pipeline, and the second end hole of each flow channel is connected to one of the second liquid in / out ports through a fluid pipeline;

[0028] One of the first liquid in / out ports is connected to a selection port of the first selection valve assembly, and one of the second liquid in / out ports is connected to the common port of one of the second selection valve assemblies.

[0029] In a second aspect, the present application provides a gene sequencer, which includes any one of the above gene sequencing fluid systems, an optical detection system, and a computer system; wherein:

[0030] A sequencing object is received within a flow channel of a gene sequencing fluid system. The sequencing object contacts a reagent and undergoes a chemical reaction to carry a fluorescent label;

[0031] An optical detection system configured to excite the fluorescent label carried by the sequencing object and detect the fluorescent signal generated by the excitation of the fluorescent label;

[0032] A computer system configured to obtain a fluorescent image from the optical detection system and identify the gene sequence of the sequencing object based on the fluorescent image.

[0033] In the gene sequencing fluid system of the above gene sequencer, both the waste liquid storage and the flow cell assembly can be connected to the pump assembly through pipelines only by connecting to the first selection valve assembly. At the same time, the second selection valve assembly can selectively connect multiple reagent holes of the reagent storage to the flow cell assembly. By using the selection functions of the first selection valve assembly and the second selection valve assembly, a large number of connecting pipelines can be saved, thereby saving pipeline layout space and improving the integration degree of the gene sequencing fluid system. Description of the Drawings

[0034] Figure 1 Overall schematic diagram of the gene sequencing fluid system in the embodiment of the present application;

[0035] Figure 2 Integrated schematic diagram of the pump assembly and the first selection valve assembly in the embodiment of the present application;

[0036] Figure 3 Second schematic diagram of the access of two flow channels in the embodiment of the present application;

[0037] Figure 4 Third schematic diagram of two flow channels in the embodiment of the present application;

[0038] Figure 5 Fourth schematic diagram of two flow channels in the embodiment of the present application. Detailed Description of the Embodiment

[0039] In order to make the objectives, technical solutions, and advantages of the present application clearer, the present application will be further described in detail below with reference to the accompanying drawings.

[0040] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in one or more embodiments of this specification should have the ordinary meanings understood by those of ordinary skill in the art to which this disclosure pertains. The terms "first", "second" and similar terms used in one or more embodiments of this specification do not denote any order, quantity or importance, but are only used to distinguish different components. The terms such as "including" or "comprising" mean that the elements or objects appearing before this word cover the elements or objects listed after this word and their equivalents, without excluding other elements or objects. The terms such as "connected" or "coupled" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. The terms such as "upper", "lower", "left" and "right" are only used to represent relative positional relationships, and when the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0041] To facilitate the understanding of the gene sequencing fluid system provided in the embodiments of the present application, its application scenario will be described first. The gene sequencing fluid system provided in the embodiments of the present application can be applied to a gene sequencer. The gene sequencing fluid system is an important functional part for the gene sequencer to complete gene sequencing. Usually on a gene sequencer, a series of biochemical reactions that occur during gene sequencing are all carried out within the gene sequencing fluid system, which requires the gene sequencing fluid system to implement many functions such as liquid inlet, liquid discharge, airtightness inspection, and pipeline self-cleaning. To implement these many functions, the gene sequencing fluid system includes many functional devices. However, in the prior art, the pipelines connecting these functional devices are numerous and the connection relationships are complex, resulting in a low integration degree of the overall gene sequencing fluid system. Therefore, the present application provides a gene sequencing fluid system to improve the integration degree of the gene sequencing fluid system. The gene sequencing fluid system provided in the present application will be specifically introduced below with reference to the accompanying drawings.

[0042] Refer to Figure 1 , Figure 1 is one of the overall schematic diagrams of the gene sequencing fluid system in the embodiments of the present application. The gene sequencing fluid system includes a reagent storage (not shown in the figure), a second selection valve assembly 1, a flow cell assembly 2, a pump assembly 3, a waste liquid storage 5, and a first selection valve assembly 7.

[0043] Among them, the biochemical reactions that occur during gene sequencing are all carried out in the flow cell assembly 2. The two opposite ends of the flow cell assembly 2 respectively have a first liquid inlet / outlet 21 and a second liquid inlet / outlet 22 for fluid to enter. Reagents, cleaning liquids, waste liquids, air, etc. can all flow into and out of the flow cell assembly 2 through the first liquid inlet / outlet 21 or the second liquid inlet / outlet 22 of the flow cell assembly 2. The waste liquid storage 5 can store the waste liquid generated during gene sequencing.

[0044] The pump assembly 3 is the power source of the gene sequencing fluid system; when the pump assembly 3 is in the suction state, it can suck the fluid outside the pump assembly 3 into the pump assembly 3 and store the fluid inside the pump assembly 3; when the pump assembly 3 is in the discharge state, the pump assembly 3 can discharge the fluid stored inside the pump assembly 3 out of the pump assembly 3.

[0045] The first selection valve assembly 7 has a plurality of selection ports and a common port 71. The first liquid inlet / outlet 21 and the waste liquid storage 5 are respectively and individually connected to a selection port of the first selection valve assembly 7 through fluid pipelines. The pump assembly 3 is connected to the common port of the first selection valve assembly 7, and the first selection valve assembly 7 is configured to selectively communicate the pump assembly 3 with the first liquid inlet / outlet 21, the waste liquid storage 5, and the air. Specifically, a selection port of the first selection valve assembly 7 is directly connected to the air, and the first selection valve assembly 7 can selectively communicate the common port 71 with a plurality of selection ports. Together with the pump assembly 3 being in the suction state or the discharge state, the inlet and discharge of the reagent are realized.

[0046] When specifically connecting the flow cell assembly 2, the pump assembly 3, the waste liquid storage 5, and the first selection valve assembly 7 through fluid pipelines, the first selection valve assembly 7 may have a common port 71, a selection port 72, a selection port 74, and a selection port 75. Among them, the common port 71 is connected to the pump assembly 3 through a fluid pipeline, the selection port 72 is connected to the waste liquid storage 5 through a fluid management, the selection port 74 communicates with the external space of the gene sequencing fluid system, that is, the selection port 74 communicates with the air, and the selection port 75 is connected to the first liquid inlet / outlet 21 of the flow cell assembly 2 through a fluid pipeline.

[0047] When the gene sequencing fluid system is running, the first selection valve assembly 7 can selectively communicate the common port 71 with the remaining plurality of selection ports. Moreover, when the common port 71 is in communication with a certain selection port, the other selection ports are in a closed and non - communicating state with the common port 71. For example, when the common port 71 is in communication with the selection port 72, both the selection port 74 and the selection port 75 are in a closed and non - communicating state with the common port 71.

[0048] The reagent storage includes a plurality of reagent holes, which can be used to store various reagents required for gene sequencing. These reagent holes can be connected to the flow cell assembly 2 through the second selection valve assembly 1. Specifically, the second selection valve assembly 1 also has a plurality of selection ports and a common port. The second liquid inlet / outlet 22 is connected to a common port of the second selection valve assembly 1 through a fluid pipeline. The plurality of reagent holes are respectively connected to the plurality of selection ports of the second selection valve assembly 1 through fluid pipelines. The second selection valve assembly 1 is configured to selectively communicate the second liquid inlet / outlet 21 with any one of the plurality of reagent holes and the air.

[0049] During the gene sequencing process, a variety of reagents are required. The second selection valve assembly 1 can selectively introduce specific reagents into the flow cell assembly 2, and at some times, air needs to be pumped into the gene sequencing fluid system through the second selection valve assembly 1, and at some times, liquid needs to be discharged to the outside through the second selection valve assembly 1.

[0050] As an example, the second selection valve assembly 2 may include several liquid extraction needles. Each liquid extraction needle is connected to a selection port of the second selection valve assembly 1, and the common port of the second selection valve assembly 1 can be selectively connected to these selection ports. When extracting reagents from the reagent storage, these liquid extraction needles can penetrate the protective film covering the reagent holes and enter the storage space of the reagent storage to extract reagents. The specific number of liquid extraction needles can be determined according to the types of reagents required for the actual gene sequencing reaction.

[0051] Optionally, when the gene sequencing fluid system is operating, the first selection valve assembly 7 can have multiple working positions to enable the pump assembly 3 to remain connected to different components. Specifically, when the first selection valve assembly 7 is in the first working position, the common port 71 is connected to the selection port 75, that is, the pump assembly 3 is connected to the flow cell assembly 2; when the first selection valve assembly 7 is in the second working position, the common port 71 is connected to the selection port 72, that is, the pump assembly 3 is connected to the waste liquid storage 5; when the first selection valve assembly 7 is in the fourth working position, the common port 71 is connected to the selection port 74, that is, the pump assembly 3 is connected to the external space.

[0052] When the first selection valve assembly 7 is in different working positions, the first selection valve assembly 7 can cooperate with the suction state or discharge state of the pump assembly 3 to achieve functions such as liquid suction and waste liquid discharge in the gene sequencing fluid system.

[0053] In a specific embodiment of the present application, when the gene sequencing fluid system is about to perform sequencing work, the first selection valve assembly 7 can be in the first working position, the pump assembly 3 is connected to the flow cell assembly 2, and at the same time, the pump assembly 3 is in the suction state. At this time, under the suction of the pump assembly 3, through the selection of the second selection valve assembly 1, specific reagents can enter the flow cell assembly 2, and then biochemical reactions occur in the flow cell assembly 2.

[0054] After the biochemical reaction and the optical system of the gene sequencer finish taking pictures, the reagents in the flow cell after the biochemical reaction become waste liquid. Continuing to keep the first selection valve assembly 7 in the first working position and the pump assembly 3 in the suction state, the waste liquid in the flow cell assembly 3 can be pumped into the pump assembly 3 for temporary storage.

[0055] When the waste liquid in the pump assembly 3 reaches a certain amount, the first selection valve assembly 7 can be switched to the second working position. The pump assembly 3 remains connected to the waste liquid storage 5, and at the same time, the pump assembly 3 is in the discharge state, so that the waste liquid temporarily stored inside the pump assembly 3 can be discharged to the waste liquid storage 5.

[0056] When the first selection valve assembly 7 is in the fourth working position, the pump assembly 3 is connected to the air, and at the same time, the pump assembly 3 can be in the suction state to suck air from the external space and temporarily store it in the pump assembly 3 for subsequent airtightness detection or drying operation of the pipeline, etc.

[0057] For the above gene sequencing fluid system, the waste liquid storage 5 and the flow cell assembly 2 can be connected to the pump assembly 3 through pipelines only by connecting to the first selection valve assembly 7. At the same time, the second selection valve assembly 1 can selectively connect multiple reagent holes of the reagent storage to the flow cell assembly 2. By using the selection functions of the first selection valve assembly 7 and the second selection valve assembly 1, a large number of connecting pipelines can be saved, thereby saving the pipeline layout space and improving the integration degree of the gene sequencing fluid system.

[0058] As an optional implementation manner, the gene sequencing fluid system provided by the embodiment of the present application further includes a cleaning liquid storage 4, which can store the cleaning liquid for cleaning the gene sequencing fluid system. The cleaning liquid storage 4 is connected to a selection port of the first selection valve assembly 7 through a fluid pipeline. The first selection valve assembly 7 is further configured to selectively connect the cleaning liquid storage 4 to the pump assembly 3 and selectively connect the pump assembly 3 to the first liquid inlet / outlet 21.

[0059] The first selection valve assembly 7 controls the cleaning liquid storage 4 to be connected to the pump assembly 3 and controls the pump assembly 3 to be connected to the first liquid inlet / outlet 21, and cooperates with the pump assembly 3 to be in the suction or discharge state to realize the cleaning function of the gene sequencing fluid system.

[0060] Specifically, the first selection valve assembly 7 further has a selection port 73, and the selection port 73 is connected to the cleaning liquid storage 4 through a fluid pipeline. The first selection valve assembly 7 can also be in the third working position. When the first selection valve assembly 7 is in the third working position, the common port 71 is connected to the selection port 73, that is, the pump assembly 3 is connected to the cleaning liquid storage 4.

[0061] Switch the first selection valve assembly 7 to the third working position and keep the pump assembly 3 in the suction state. Under the suction action of the pump assembly 3, the cleaning liquid stored in the cleaning liquid storage 4 can be sucked into the pump assembly 3 for temporary storage. After a certain amount of cleaning liquid is stored in the pump assembly 3, switch the first selection valve assembly 7 to the first working position and make the pump assembly 3 in the discharge state, then the cleaning liquid temporarily stored in the pump assembly 3 can be discharged from the gene sequencing fluid system through the flow cell assembly 2 and the second selection valve assembly 1. During the process of discharging the cleaning liquid from the gene sequencing fluid system, a cleaning of the passage including the pump assembly 3, the flow cell assembly 2, the first selection valve assembly 7, the second selection valve assembly 1 and the related fluid pipelines is completed. Of course, in order to obtain a better cleaning effect, the passage including the pump assembly 3, the flow cell assembly 2, the first selection valve assembly 7, the second selection valve assembly 1 and the related fluid pipelines can be cleaned multiple times. The process of multiple cleaning is to repeat the above cleaning steps, which will not be elaborated here.

[0062] By using the selection function of the first selection valve assembly 7, the pipeline layout requirements of the cleaning liquid storage 4 can be reduced, and the integration degree of the gene sequencing fluid system can be improved.

[0063] As an optional implementation manner, the gene sequencing fluid system provided in the embodiment of the present application further includes an airtightness detection device 6, and the airtightness detection device 6 is arranged on the fluid pipeline between a selection port of the first selection valve assembly 7 and a selection port of the second selection valve assembly 1; the airtightness detection device 6 is configured to detect the airtightness of the loop where it is located.

[0064] Specifically, one end of the airtightness detection device 6 is connected to a selection port of the first selection valve assembly 7 through a fluid pipeline, and the opposite end of the airtightness detection device 6 is connected to a selection port of the second selection valve assembly 1 through a fluid pipeline. At the same time, the first selection valve assembly 6 is further configured to selectively keep the airtightness detection device 6 in communication with the pump assembly 3, or keep the airtightness detection device 6 in communication with the first liquid inlet / outlet 21; the second selection valve assembly 1 is further configured to selectively communicate the airtightness detection device 6 with the second liquid inlet / outlet 21, or close the port in communication with the airtightness detection device 6.

[0065] Specifically, the first selection valve assembly 7 further has a selection port 76, and the selection port 76 is connected to the airtightness detection device 6 through a fluid pipeline. The first selection valve assembly 7 can also be in the fifth working position. When the first selection valve assembly 7 is in the fifth working position, the common port 71 is kept in communication with the selection port 76, that is, the pump assembly 3 is kept in communication with the airtightness detection device 6.

[0066] When performing an airtightness test on the pipeline, first switch the first selection valve assembly 7 to the fourth working position and make the pump assembly 3 in the suction state. At this time, the pump assembly 3 is in communication with the air in the external space, and the air enters the pump assembly 3 through the selection port 74 and the common port 71 and is temporarily stored in the pump assembly 3. After a certain amount of air is temporarily stored in the pump assembly 3, switch the first selection valve assembly 7 to the fifth working position and make the pump assembly 3 in the discharge state. At this time, the airtightness detection device 6 is in communication with the flow cell assembly 2, and the pump assembly 3 pumps the air temporarily stored in the pump assembly 3 into the passage composed of the airtightness detection device 6, the second selection valve assembly 1, the flow cell assembly 2, and the second selection valve assembly 7, and the airtightness detection can be performed by the airtightness detection device 6.

[0067] By performing an airtightness test on the gene detection fluid system, the risk of bubbles being mixed into the reagent due to the poor airtightness of the fluid system when the pump assembly 3 sucks the reagent can be reduced. At the same time, by using the selection function of the first selection valve assembly 7 and the second selection valve assembly 1, the pipeline layout requirements of the airtightness detection device 6 can be reduced, and the integration degree of the gene sequencing fluid system can be improved.

[0068] The specific process of the airtightness test can be that after pumping air into the passage composed of the airtightness detection device 6, the second selection valve assembly 1, and the flow cell assembly 2, close the selection port 75 and the selection port 76. At this time, the air is trapped in this closed pipeline. By detecting whether the gas pressure in the closed pipeline drops after a period of time by the airtightness detection device 6, it can be judged whether the airtightness of this section of the closed pipeline is intact.

[0069] As an alternative embodiment, the gene sequencing fluid system further includes a bubble sensor 9, and the bubble sensor 9 is arranged on the fluid pipeline between the waste liquid storage 5 and a selection port of the first selection valve assembly 7. It is not difficult to understand that the selection port here is the selection port 72 of the first selection valve assembly 7 for communicating with the waste liquid storage 5. The bubble sensor 9 is configured to detect the number of bubbles in the fluid to be discharged into the waste liquid storage 5, and thus the quality of the fluid entering the flow cell assembly 2 can be inferred.

[0070] By setting the bubble sensor 9, it is convenient to timely understand the quality of the sample injection liquid of the gene sequencing fluid system, and thus the situation that the gene sequencing result is affected by bubbles mixed in the sample injection process can be timely discovered, and the risk of a large number of detection results being unavailable due to bubbles mixed in the sample injection process can be reduced. At the same time, by arranging the bubble sensor 9 on the fluid pipeline between the waste liquid storage 5 and the first selection valve assembly 7, no additional pipeline needs to be arranged, which helps to improve the integration degree of the gene sequencing fluid system.

[0071] As an alternative embodiment, in the gene sequencing fluid system provided by the embodiments of the present application, the first selection valve assembly 7 and the pump assembly 3 can be integrated into one body. Refer to Figure 2 , Figure 2 which is a schematic diagram of the integration of the pump assembly 3 and the first selection valve assembly 7 in the gene sequencing fluid system provided by the embodiments of the present application. As shown in Figure 2 , this integrated component can not only realize the suction, discharge and fluid temporary storage functions of the pump assembly 3, but also selectively communicate with the external space, the cleaning liquid storage 4, the waste liquid storage 5, the airtightness detection device 6 and the flow cell assembly 2 one by one. By integrating the first selection valve assembly 7 and the pump assembly 3 into one body, the connecting pipeline between the first port 71 and the pump assembly 3 and the space required for the pipeline can be saved, which helps to improve the integration degree of the gene sequencing fluid system. The assembly and parts management costs can also be saved through the integrated component.

[0072] When specifically implementing the gene sequencing fluid system provided by this embodiment, it is possible to set only one flow channel 23 in the flow cell assembly 2, or it is also possible to set that the flow cell assembly 2 includes multiple flow channels 23. Moreover, when the flow cell assembly 2 includes multiple flow channels 23, the multiple flow channels 23 can be connected to the gene sequencing fluid system in different ways.

[0073] As an alternative embodiment, the flow cell assembly 2 provided by the embodiments of the present application includes an even number of flow channels 23 greater than one, and these flow channels are connected in parallel between the first liquid inlet / outlet 21 and the second liquid inlet / outlet 22. Specifically, both opposite ends of each flow channel 23 have a first end hole 231 and a second end hole 232 through which fluid can enter and exit. The first end holes 231 of every two flow channels 23 are connected to a first liquid inlet / outlet 21 through a manifold pipeline, and this first liquid inlet / outlet 21 is also connected to a selection port 75 of the first selection valve assembly. At the same time, the second end holes 232 of every two flow channels 23 are connected to a second liquid inlet / outlet 22 through a manifold pipeline, and this second liquid inlet / outlet is also connected to a common port 111 of a second selection valve assembly 1.

[0074] As can be easily understood from the above description, when specifically setting the flow channels 23, the number of flow channels 23 can be two, four, eight, etc. When specifically setting these flow channels 23, every two flow channels 23 share the same first liquid inlet / outlet 21 and the second liquid inlet / outlet 22. Therefore, when the number of flow channels 23 is two, the number of the first liquid inlet / outlet 21 and the second liquid inlet / outlet 22 of the flow cell assembly 2 can be one each, which can meet the connection requirements of the two flow channels 23. When the number of flow channels 23 is greater than two, that is, the number of the first liquid inlet / outlet 21 and the second liquid inlet / outlet 22 needs to be increased synchronously. For example, if the number of flow channels 23 is 4, two first liquid inlet / outlets 21 and two second liquid inlet / outlets 22 are required to meet the connection requirements of all flow channels 23.

[0075] Optionally, when matching more flow channels 23, the number of the first liquid inlet / outlet 21 and the second liquid inlet / outlet 22 also increases synchronously, and at the same time, the number of the first selection valve assembly 7, the pump assembly 3, and the second selection valve assembly 1 also increases synchronously and matches. The increase relationship can be: every two flow channels 23 correspond to one first selection valve assembly 7, one second selection valve assembly 1, and one pump assembly 3. For example, when the number of flow channels 23 is 4, the number of the pump assembly 3, the first selection valve assembly 7, and the second selection valve assembly 1 are all two.

[0076] For other components in the gene sequencing fluid system, such as the waste liquid storage 5, the cleaning liquid storage 4, the airtightness detection device 6, the bubble sensor 9, etc., they do not have to increase synchronously with the increase in the number of flow channels 23. Only one set of the above components can be set in the gene sequencing fluid system to cooperate with all flow channels 23, all pump assemblies 3, all first selection valve assemblies 7, and all second selection valve assemblies 1.

[0077] Figure 1 An example of a connection situation when the flow cell assembly 2 includes two flow channels 23 is shown. For the cases where the number of flow channels is 4, 8 or more, the corresponding components can be increased synchronously with reference to Figure 1 the form.

[0078] As Figure 1 shown, the number of the first liquid inlet / outlet 21 and the second liquid inlet / outlet 22 of the flow cell assembly 2 is one each. The first end hole 231 of each flow channel 23 is connected to one first liquid inlet / outlet 21, and the second end hole 232 of each flow channel 23 is connected to one second liquid inlet / outlet 22. Specifically, connecting the two first end holes 231 to the first liquid inlet / outlet 21 can be achieved through a three-way manifold. Similarly, connecting the two second end holes 232 to one second liquid inlet / outlet 22 can also be achieved through a three-way manifold.

[0079] The second selection valve assembly 1 includes a second selection valve 11. The common port and the selection port of the second selection valve assembly 1 both originate from the second selection valve 11. Specifically, the selection port 112 communicates with the airtightness detection device 6, and the common port 111 communicates with the second liquid inlet / outlet 22.

[0080] The common port 111 is also used to selectively communicate with the reagent holes of the reagent storage. That is, in normal sequencing operations, the second liquid inlet / outlet 22 can communicate with one of the multiple reagent holes through the common port 111 to extract the required reagent. In a specific implementation, the common port 111 selectively communicates with one of the multiple reagent holes. When specifically setting up the reagent storage, the number of its reagent holes may be 10, 20, 24, 28, etc. according to actual needs. Correspondingly, the connection between the common port 111 and the reagent storage may be 10 channels, 20 channels, 24 channels, 28 channels, etc., which can be specifically determined according to the actual sequencing requirements.

[0081] During normal sequencing or cleaning operations, the selection port 112 is in a closed state, that is, the airtightness detection device 6 is not connected to the flow channel 23. This can reduce the risk of reagent or cleaning liquid entering the airtightness detection device during sequencing or cleaning operations, and further reduce the risk of damage or accuracy decline of the airtightness detection device 6 due to contamination by reagent or cleaning liquid, etc.

[0082] When detecting the airtightness of the gene detection fluid system, the selection port 112 is in an open state, and at the same time, the connection between the common port 111 and the reagent storage is disconnected. At this time, the pump assembly 3 can pump gas from the common port 71 through the selection port 75 and the first liquid inlet / outlet 21 into the two flow channels 23, and then the gas passes through the second liquid inlet / outlet 22, the common port 111, the selection port 112, the airtightness detection device 6, and the selection port 72, making the above gas flow path form a closed loop, and thus the airtightness of this closed loop can be detected.

[0083] When it is necessary to extract air through the second valve selection valve assembly 1, the second selection valve 11 can connect the selection port 113 to the common port 111 to extract air into the flow channel 23.

[0084] During the process of gene sequencing, all the flow channels 23 connected in parallel can perform the same operations simultaneously. For example, multiple flow channels 23 can simultaneously feed liquid, discharge waste liquid, clean, etc. By adopting the method of multiple flow channels 23 operating simultaneously, multiple groups of biochemical reactions can be carried out at the same time, and the gene detection efficiency is almost doubled. At the same time, multiple flow channels 23 share a set of liquid inlet / outlet gene detection liquid path systems, improving the integration of the gene detection fluid system.

[0085] Of course, in specific implementation, the method of arranging multiple flow channels 23 in the flow cell assembly 2 is not limited to the above-mentioned parallel connection of multiple flow channels 23.

[0086] As an alternative implementation, when the number of flow channels 23 is an even number greater than one, each of the opposite ends of each flow channel 23 has a first end hole 231 and a second end hole 232 for fluid inlet and outlet. The first end hole 231 of each flow channel 23 is connected to a first liquid inlet / outlet 21 through a fluid pipeline, and the second end hole 231 of each flow channel 23 is connected to a second liquid inlet / outlet 22 through a fluid pipeline. And a first liquid inlet / outlet 21 is connected to a selection port of a first selection valve assembly 7, and a second liquid inlet / outlet 22 is connected to a common port 111 of a second selection valve assembly 1.

[0087] For such a connection method, the numbers of the flow channels 23, the first liquid inlet / outlets 21, and the second liquid inlet / outlets 22 correspond one by one. Therefore, an increase or decrease in the number of flow channels 23 will necessarily be accompanied by an increase in the numbers of the first liquid inlet / outlets 21 and the second liquid inlet / outlets 22. In such a connection method, the pump assembly 3, the first selection valve assembly 7, the flow channels 23, and the first selection valve are arranged in a certain proportion. For example, in a matching method similar to the above, every two flow channels 23 correspond to a first selection valve assembly 7, a pump assembly 3, and a second selection valve assembly 1.

[0088] For two of the flow channels 23, both of the two flow channels 23 are respectively connected to a selection port of the first selection valve assembly 7, and the first selection valve assembly 7 can selectively connect the common port 71 with one of the two selection ports connected to the flow channels 23. And each second selection valve assembly 1 may include two second selection valves 11. Each second selection valve 11 has a common port 111, and each common port is used to communicate with a second liquid inlet / outlet 22. And each second selection valve 111 respectively has a plurality of selection ports to communicate with reagent holes, an airtightness detection device 6, and air.

[0089] Reference Figure 3 , Figure 3 is the second schematic diagram of the access of two flow channels in the embodiment of the present application. Figure 3 Only the access situation of two flow channels 23 is shown in Figure 3 . If more flow channels 23 are accessed, the pump assembly 3, the first selection valve assembly 7, and the second selection valve assembly 1 can be synchronously increased with reference to the access method in Figure 3As shown, the flow cell assembly 2 includes two flow channels 23. Meanwhile, the flow cell assembly 2 has two first liquid in / out ports 21 and two second liquid in / out ports 22. For any one of the flow channels 23, the first end hole 231 of this flow channel 23 communicates with one of the first liquid in / out ports 21, and one of the second end holes 232 of this flow channel 23 communicates with one of the second liquid in / out ports 22.

[0090] The first selection valve assembly 1 further includes a selection port 77. The selection port 75 and the selection port 77 communicate with the two first end holes 231 of the two flow channels 23 in a one-to-one correspondence. When the first selection valve assembly 7 is in the first working position, the common port 71 can communicate with either the selection port 75 or the selection port 77.

[0091] The second selection valve assembly 1 includes two second selection valves 11 corresponding to the two flow channels 23, and each second selection valve 11 has a common port 111, a selection port 112, and a selection port 113. The common port 111 of each second selection valve 11 communicates with one of the second liquid in / out ports 22; each selection port 112 communicates with the airtightness detection device 6. And each common port 111 can communicate with all the reagent holes selectively, so that each flow channel 23 can select the required reagent through the common port 111.

[0092] For the specific connection mode of the common port 111 with the reagent storage, the opening and closing conditions of the selection port 112 during the cleaning operation, and the method of extracting air through the selection port 113, reference can be made to the above description and will not be elaborated here.

[0093] Each selection port 112 communicates with the airtightness detection device 6. Specifically, a fluid pipeline can start from the selection port 72, and then after passing through the airtightness detection device, it successively communicates with all the selection ports 112. During the airtightness detection, the selection port 112 is in an open state, and at the same time, the connection between the common port 111 and the reagent storage is disconnected. At this time, the pump assembly 3 can pump gas from the common port 71 through one of the selection port 75 and the selection port 77 into one of the flow channels 23. Then the gas reaches the corresponding second liquid in / out port 22, the common port 111, and the selection port 112 of this flow channel 23. After reaching this selection port 112, part of the gas enters another flow channel 23 through another selection port 112, and another part of the gas reaches the selection port 72 through the airtightness detection device 6. At this time, the pump assembly 3, the first selection valve assembly 7, the flow cell assembly 2, the first selection valve assembly 1, and the airtightness detection device 6 form a closed loop, and thus the airtightness of this closed loop can be detected.

[0094] During the specific sequencing, the liquid inlet of multiple flow channels 23 can be carried out alternately. For example Figure 3In the case where the flow cell assembly 2 shown includes two flow channels 23, during the sequencing operation, the selection port 71 can be first connected to the selection port 75 to extract the reagent to the flow channel 23 corresponding to the selection port 75; during the time when a biochemical reaction occurs in this flow channel 23 and when photographing the first flow channel after the reaction ends, the first selection valve assembly 7 can be switched to connect the first port 71 to the second sub-port 752, and extract the reagent into the flow channel 23 corresponding to the selection port 77; during the time when a biochemical reaction occurs in the flow channel 23 corresponding to the selection port 77 and when photographing this flow channel 23 after the reaction ends, the first selection valve assembly 7 can be switched back to connect the first port 71 to the first sub-port 751, and extract the waste liquid in the flow channel 23 corresponding to the first sub-port 751 to the pump assembly 3, and at the same time, new reagent can be extracted into the flow channel 23 corresponding to the first sub-port 751; in such a cycle, the operations of feeding liquid and discharging waste liquid can be alternately performed on the two flow channels 23.

[0095] In such an alternative sampling method, when the number of flow channels 23 is large and the imaging field of the photographing camera cannot cover all the flow channels 23, the flow channels 23 can be alternately sampled, improving the gene sequencing efficiency. In addition, when adopting the alternative sampling method, the sampling of each flow channel 23 is realized separately, which is beneficial to accurately controlling the dosage of the reagent entering each flow channel 23 and is beneficial to improving the stability of the gene sequencing fluid system.

[0096] In specific implementation, there can also be other alternative sampling schemes.

[0097] As an optional implementation manner, the flow cell assembly 2 provided in the embodiment of the present application includes an even number of flow channels 23 greater than one, and both opposite ends of each flow channel 23 have a first end hole 231 and a second end hole 232 for fluid inlet and outlet. The first end hole 231 of each flow channel 23 is connected to a first liquid inlet / outlet 21 through a fluid pipeline, and one first liquid inlet / outlet 21 is connected to a selection port of the first selection valve assembly 7. The second end holes 232 of every two flow channels 23 are connected to a second liquid inlet / outlet 22 through a manifold pipeline, and this second liquid inlet / outlet is also connected to a common port 111 of a second selection valve assembly 1.

[0098] For such a connection method, the number of flow channels 23 and the first liquid inlet / outlets 21 are in one-to-one correspondence, and two flow channels 23 correspond to one second liquid inlet / outlet 22. In such a connection method, it is also possible to set that every two flow channels 23 correspond to a first selection valve assembly 7, a second selection valve assembly 1, and a pump assembly 3; and, the entire gene sequencing fluid system can share a set of waste liquid storage 5, cleaning liquid storage 4, airtightness detection device 6, and bubble sensor 9.

[0099] For two of the flow channels 23, both of the two flow channels 23 are respectively connected to a selection port of the first selection valve assembly 7. The first selection valve assembly 7 can selectively connect the common port 71 with one of the two selection ports connected to the flow channels 23. At the same time, the second end holes 232 of every two flow channels 23 are connected to a second liquid inlet / outlet 22 through a manifold pipeline, and the second liquid inlet / outlet is also connected to the common port 111 of a second selection valve assembly 1.

[0100] Figure 4 This is the second schematic diagram of the access of two flow channels in the embodiment of the present application. Figure 4 The access situation of two flow channels 23 is also shown therein. If more flow channels 23 are accessed, the pump assembly 3, the first selection valve assembly 7, and the second selection valve assembly 1 can be synchronously increased with reference to the access method in Figure 4 That's it.

[0101] As Figure 4 shown, the flow cell assembly 2 includes two flow channels 23. At the same time, the flow cell assembly 2 has two first liquid inlet / outlets 21 and a second liquid inlet / outlet 22; the first end holes 231 of the two flow channels 23 are respectively connected to a first liquid inlet / outlet 21, and the two second end holes 232 of the two flow channels 23 are connected to the second liquid inlet / outlet 22 through a manifold at the same time.

[0102] The first selection valve assembly 1 further includes a selection port 77. The selection port 75 and the selection port 77 are in one-to-one correspondence with the two first end holes 231 of the two flow channels 23. When the first selection valve assembly 7 is in the first working position, the common port 71 can be connected to one of the selection port 75 or the selection port 77.

[0103] The second selection valve assembly 1 includes a second selection valve 11. The common port and the selection ports of the second selection valve assembly 1 are all issued by the second selection valve 11. Specifically, the selection port 112 is connected to the airtightness detection device 6, and the common port 111 is connected to the second liquid inlet / outlet 22.

[0104] For the specific connection method of the common port 111 to the reagent storage, the opening and closing situation of the selection port 112 during the cleaning operation, and the method of extracting air through the selection port 113, reference can be made to the above description, which will not be elaborated here.

[0105] When detecting the airtightness of the gene detection fluid system, the selection port 112 is selected to be in an open state, and at the same time, the communication between the common port 111 and the reagent storage is disconnected. At this time, the pump assembly 3 can pump gas from the common port 71 into a flow channel 23 through one of the selection ports 75 and 77, and then the gas reaches the second liquid inlet / outlet 22 and is divided into two paths. One path enters another flow channel, and the other path passes through the common port 111, the selection port 112, the airtightness detection device 6 and reaches the selection port 72. At this time, the pump assembly 3, the first selection valve assembly 7, the flow cell assembly 2, the first selection valve assembly 1 and the airtightness detection device 6 form a closed loop, and thus the airtightness of this closed loop can be detected.

[0106] In this way, alternative sample injection of multiple flow channels 23 can also be achieved, and the same benefits as the above alternative sample injection scheme can be obtained.

[0107] As an alternative embodiment, the flow cell assembly 2 provided in the embodiments of the present application includes an even number of flow channels 23 greater than one. Both opposite ends of each flow channel 23 have a first end hole 231 and a second end hole 232 for fluid inlet and outlet. The first end holes 231 of every two flow channels 23 are connected to a first liquid inlet / outlet 21 through a manifold pipeline, and the first liquid inlet / outlet 21 is also connected to a selection port 75 of the first selection valve assembly; at the same time, the second end holes 231 of each flow channel 23 are connected to a second liquid inlet / outlet 22 through a fluid pipeline, and a second liquid inlet / outlet 22 is connected to a common port 111 of a second selection valve assembly 1.

[0108] For such a connection method, the number of flow channels 23 and the second liquid inlet / outlet 22 correspond one by one, and two flow channels 23 correspond to one first liquid inlet / outlet 21. In such a connection method, it is also possible to set that every two flow channels 23 correspond to a first selection valve assembly 7, a second selection valve assembly 1 and a pump assembly 3; and the entire gene sequencing fluid system can share a set of waste liquid storage 5, cleaning liquid storage 4, airtightness detection device 6 and bubble sensor 9.

[0109] For two of the flow channels 23, the first end holes 231 of the two flow channels 23 are connected to a first liquid inlet / outlet 21 through a manifold pipeline, and the first liquid inlet / outlet 21 is also connected to a selection port 75 of a first selection valve assembly 1. And each second selection valve assembly 1 may include two second selection valves 11. Each second selection valve 11 has a common port 111, and each common port is used to communicate with a second liquid inlet / outlet 22, and each second selection valve 111 respectively has a plurality of selection ports to communicate with reagent holes, the airtightness detection device 6 and air.

[0110] Figure 5 This is the third schematic diagram of the access of two flow channels in the embodiments of the present application. Figure 5 The access situation of the two flow channels 23 is also shown in the figure. If there are more flow channels 23 to be accessed, the pump assembly 3, the first selection valve assembly 7, and the second selection valve assembly 1 can be synchronously increased with reference to the access method in Figure 5 the figure.

[0111] As Figure 5 shown, the flow cell assembly 2 includes two flow channels 23. At the same time, the flow cell assembly 2 has a first liquid inlet / outlet 21 and two second liquid inlets / outlets 22. The two first end holes 231 of the two flow channels 23 are simultaneously communicated with a first liquid inlet / outlet 21 through a manifold, and the two second end holes 232 of the two flow channels 23 are respectively communicated with the two second liquid inlets / outlets 22 in one-to-one correspondence.

[0112] As Figure 1 shown, the number of the first liquid inlet / outlets 21 of the flow cell assembly 2 is one. The two first end holes 231 of the two flow channels 23 lead to a first liquid inlet / outlet 21, and the second end hole 232 of each flow channel 23 is respectively communicated with a second liquid inlet / outlet 22.

[0113] The second selection valve assembly 1 includes two second selection valves 11 corresponding to the two flow channels 23, and each second selection valve 11 has a common port 111, a selection port 112, and a selection port 113. The common port 111 of each second selection valve 11 is communicated with a second liquid inlet / outlet 22. Each selection port 112 is communicated with the airtightness detection device 6. And each common port 111 can be selectively communicated with all the reagent holes, so that each flow channel 23 can select the required reagent through the common port 111.

[0114] For the specific connection method of the common port 111 and the reagent storage, the opening and closing conditions of the selection port 112 during the cleaning operation, and the method of extracting air through the selection port 113, reference can be made to the above description and will not be elaborated here.

[0115] Each selection port 112 is connected to the airtightness detection device 6. Specifically, a fluid pipeline can be emitted from the selection port 72, and then after passing through the airtightness detection device, all the selection ports 112 are connected in sequence. When performing airtightness detection, the selection port 112 is in an open state, and at the same time, the connection between the common port 111 and the reagent storage is disconnected. At this time, the pump assembly 3 can pump gas from the common port 71 into the two flow channels 23 through the selection port 75 at the same time. Then the gas reaches the second liquid inlet / outlet 22, the common port 111, and the selection port 112 corresponding to each flow channel 23, and then converges and passes through the airtightness detection device 6 to reach the selection port 72. At this time, the pump assembly 3, the first selection valve assembly 7, the flow cell assembly 2, the first selection valve assembly 1, and the airtightness detection device 6 form a closed loop, and thus the airtightness of this closed loop can be detected.

[0116] The number of the first liquid inlet / outlets 21 of the flow cell assembly 2 is one, and the number of the second liquid inlet / outlets 22 of the flow cell assembly 2 is the same as that of the flow channels 23 and they correspond one by one. One end of each flow channel 23 is connected to the first liquid inlet / outlet 21, and the opposite end of each flow channel 23 is connected to the corresponding second liquid inlet / outlet 22.

[0117] The second selection valve assembly 1 includes a second selection valve 11 corresponding to each flow channel 23, and each second selection valve 11 may include a common port 111, a selection port 112, and a selection port 113. Figure 5 This is the fourth schematic diagram of multiple flow channels in the embodiment of the present application. As Figure 5 shown, the second selection valve assembly 1 includes two second selection valves 11, and these two second selection valves 11 correspond one by one. The common port 111 of each second selection valve 11 is connected to the corresponding second liquid inlet / outlet 22 of this second selection valve 11; each selection port 112 is connected to the airtightness detection device 6. It should be noted that each common port 111 can be selectively connected to all the reagent holes, so that each flow channel 23 can select the required reagent through the common port 111.

[0118] For the specific connection manner of each common port 111 to the reagent storage and the connection manner of the multiple selection ports 112 to the airtightness detection device 6, reference can be made to the above description, and details are not described here again.

[0119] In this way, alternative sampling of multiple flow channels 23 can also be realized, and the same benefits as the above alternative sampling scheme can be obtained.

[0120] The embodiment of the present application also provides a gene sequencer, which includes any one of the above gene sequencing fluid systems, an optical detection system, and a computer system; wherein:

[0121] A sequencing target is received in a flow channel of a gene sequencing fluid system. The sequencing target contacts with a reagent and undergoes a chemical reaction to carry a fluorescent label.

[0122] An optical detection system configured to excite the fluorescent label carried by the sequencing target and detect the fluorescent signal generated by the excited fluorescent label.

[0123] A computer system configured to obtain a fluorescent image from the optical detection system and identify the gene sequence of the sequencing target based on the fluorescent image.

[0124] It should be noted here that in a gene sequencer system, the sequencing sample can be, for example, biological blood, cell tissue, etc. DNA (Deoxyribo Nucleic Acid) single strands or RNA (Ribo Nucleic Acid) single strands can be extracted and separated from the sequencing sample. In some implementations of the present application, the sequencing target can be obtained, for example, by cutting DNA single strands or single RNA strands into multiple fragments and then chemically modifying and adding adapters to the DNA fragments or RNA fragments, that is, the sequencing target can be a library obtained through a library preparation process, or the sequencing target can also be an entire DNA single strand or an entire RNA single strand, where the RNA can be obtained by transcribing DNA.

[0125] In the gene sequencing fluid system of the above gene sequencer, the waste liquid storage 5 and the flow cell assembly 2 only need to be connected to the first selection valve assembly 7 through pipelines to achieve communication with the pump assembly 3. At the same time, the second selection valve assembly 1 can selectively connect multiple reagent holes of the reagent storage to the flow cell assembly 2. By the selection functions of the first selection valve assembly 7 and the second selection valve assembly 1, a large number of connecting pipelines can be saved, thereby saving the pipeline layout space and improving the integration degree of the gene sequencing fluid system.

[0126] One or more embodiments of this specification are intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of one or more embodiments of this specification shall be included within the protection scope of this disclosure.

[0127] The above is only the specific implementation manner of this application, but the protection scope of this application is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed in this application, and all of them should be covered within the protection scope of this application. Therefore, the protection scope of this application shall be subject to the protection scope of the claims.

Claims

1. A gene sequencing fluid system, characterized in that: include: A reagent storage device, a flow cell assembly, a pump assembly, a waste liquid storage device, a first selection valve assembly, and a second selection valve assembly; the two opposite ends of the flow cell assembly are respectively provided with a first inlet / outlet and a second inlet / outlet for fluid to flow in or out; the reagent storage device comprises a plurality of reagent holes; wherein, The first selection valve assembly has a plurality of selection ports and a common port, the first liquid inlet / outlet and the waste liquid storage are respectively connected to a selection port of the first selection valve assembly through a fluid pipeline, and the pump assembly is connected to the common port of the first selection valve assembly through a fluid pipeline; the first selection valve assembly is configured to selectively connect the pump assembly to the first liquid inlet / outlet, the waste liquid storage and air; The second selection valve assembly has multiple selection ports and a common port, and the multiple reagent holes are respectively connected to a selection port of the second selection valve assembly through fluid pipelines; the second inlet / outlet liquid port is connected to the common port of the first selection valve assembly through a fluid pipeline, and the second selection valve assembly is configured to selectively connect the second inlet / outlet liquid port to any one of the multiple reagent holes and the air.

2. The gene sequencing fluid system according to claim 1, characterized in that: The gene sequencing fluid system further includes a cleaning fluid storage device, wherein the cleaning fluid storage device is connected to a selection port of the first selection valve assembly through a fluid pipeline; The first selector valve assembly is further configured to selectively connect the cleaning fluid reservoir to the pump assembly and connect the pump assembly to the first inlet / outlet port.

3. The gene sequencing fluid system according to claim 2, characterized in that: The gene sequencing fluid system also includes an airtightness detection device; The air tightness detection device is arranged in a fluid pipeline between a selection port of the first selection valve assembly and a selection port of the second selection valve assembly; The air tightness detection device is configured to detect the air tightness of the circuit in which it is located.

4. The gene sequencing fluid system according to claim 2, characterized in that: The gene sequencing fluid system further includes a bubble sensor disposed in a fluid line between the waste liquid storage and a selection port of the first selection valve assembly; The bubble sensor is configured to detect an amount of bubbles in a fluid to be discharged into a waste reservoir.

5. The gene sequencing fluid system according to claim 1, characterized in that: The first selection valve assembly is integrated with the pump assembly.

6. The gene sequencing fluid system according to any one of claims 1 to 5, characterized in that: The flow cell assembly comprises an even number of flow channels greater than one; each flow channel has a first end hole and a second end hole at opposite ends for fluid to enter and exit; The first end holes of every two flow channels are connected to one of the first inlet / outlet liquid ports through a manifold pipeline, and the second end holes of every two flow channels are connected to one of the second inlet / outlet liquid ports through a manifold pipeline; One of the first inlet / outlet liquid ports is connected to a selection port of the first selection valve assembly, and one of the second inlet / outlet liquid ports is connected to a common port of the second selection valve assembly.

7. The gene sequencing fluid system according to any one of claims 1 to 5, characterized in that: The flow cell assembly comprises an even number of flow channels greater than one; each flow channel has a first end hole and a second end hole at opposite ends for fluid to enter and exit; The first end hole of each of the flow channels is connected to one of the first liquid inlet / outlet through a fluid pipeline, and the second end hole of each of the flow channels is connected to one of the second liquid inlet / outlet through a fluid pipeline; One of the first inlet / outlet liquid ports is connected to a selection port of the first selection valve assembly, and one of the second inlet / outlet liquid ports is connected to a common port of the second selection valve assembly.

8. The gene sequencing fluid system according to any one of claims 1 to 5, characterized in that: The flow cell assembly comprises an even number of flow channels greater than one; each flow channel has a first end hole and a second end hole at opposite ends for fluid to enter and exit; The first end hole of each flow channel is connected to one of the first inlet / outlet liquid ports through a fluid pipeline, and the second end holes of every two flow channels are connected to one of the second inlet / outlet liquid ports through a manifold pipeline; One of the first inlet / outlet liquid ports is connected to a selection port of the first selection valve assembly, and one of the second inlet / outlet liquid ports is connected to a common port of the second selection valve assembly.

9. The gene sequencing fluid system according to any one of claims 1 to 5, characterized in that: The flow cell assembly comprises an even number of flow channels greater than one; each flow channel has a first end hole and a second end hole at opposite ends for fluid to enter and exit; The first end holes of every two of the flow channels are connected to one of the first inlet / outlet liquid ports through a manifold pipeline, and the second end hole of each of the flow channels is connected to one of the second inlet / outlet liquid ports through a fluid pipeline; One of the first inlet / outlet liquid ports is connected to a selection port of the first selection valve assembly, and one of the second inlet / outlet liquid ports is connected to a common port of the second selection valve assembly.

10. A gene sequencer, characterized in that: The method comprises the gene sequencing fluid system, optical detection system and computer system as claimed in any one of claims 1 to 9; wherein: A sequencing object is received in a flow channel in the gene sequencing fluid system, and the sequencing object contacts the reagent and undergoes a chemical reaction so as to carry a fluorescent label; The optical detection system is configured to excite the fluorescent marker carried by the sequencing object and detect the fluorescent signal generated by the excited fluorescent marker; The computer system is configured to acquire a fluorescent image from the optical detection system and identify a gene sequence of the sequencing object according to the fluorescent image.

Images