Automatic liquid supplementing and pouring device for cell culture bag
By designing an automatic liquid replenishment and liquid pouring device for cell culture bags, using technical means such as vacuum pumps and magnetic columns, the problem of inconvenient liquid replenishment and liquid pouring in the existing technology is solved, and efficient and safe cell culture operation is achieved.
Patent Information
- Application Number
- CN202421925909.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-09
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2034-08-09
AI Technical Summary
The prior art has inconvenient operation of rehydration and pouring liquid during cell culture, which can easily damage the cell culture bag, resulting in leakage and cell damage. The traditional rehydration method is inefficient and difficult to ensure environmental stability.
An automatic liquid replenishment and liquid pouring device for cell culture bags is designed, and the liquid is absorbed automatically by a vacuum pump. The culture bag is fixed through a magnetic column and a snap structure to achieve automatic liquid pouring without lifting or squeezing with both hands.
It improves the efficiency and safety of rehydration and pouring fluid, reduces labor intensity, avoids cell damage, ensures high cell activity, and reduces the risk of contamination.
Smart Images
Figure CN222961440U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of medical devices, and specifically relates to an automatic liquid supplementing and liquid pouring device for a cell culture bag. Background Art
[0002] Cell culture plays a crucial role in biomedical research and applications. During the cell culture process, accurately supplementing liquid to the cell culture bag and efficiently pouring out the cell suspension are key operating steps. However, there are many drawbacks in the existing technology for collecting cell fluid. For example, the method of manually holding the cell culture bag and squeezing it out not only is inconvenient to operate, but also easily damages the cell culture bag and causes liquid leakage. Moreover, this method often makes it difficult to squeeze out the cell fluid remaining at the bottom of the cell culture bag, thus increasing the time for cell collection and causing a large waste of manpower and material resources. In addition, the traditional liquid supplementing method is inefficient and difficult to ensure the stability of the environment inside the cell culture bag, which is likely to have an adverse impact on cell culture. During the liquid pouring process, cells may also be damaged due to improper operation, affecting subsequent experiments and applications. To sum up, in order to overcome these defects in the existing technology, the research and development of an innovative automatic liquid supplementing and liquid pouring device for cell culture bags has important practical significance and application value. Content of the Utility Model
[0003] The purpose of the utility model is to provide an automatic liquid supplementing and liquid pouring device for a cell culture bag, which does not require cumbersome actions of holding or squeezing with both hands, so as to solve the above problems.
[0004] To achieve the above purpose, the utility model provides the following technical solutions:
[0005] An automatic liquid supplementing and liquid pouring device for a cell culture bag includes a high platform and a low platform fixedly installed at the right bottom of the high platform. On both sides of the left position at the upper end of the low platform, two fixed columns are respectively fixedly installed. On the left side of the fixed columns and at the two right corners of the upper end of the low platform, four magnetic columns are respectively installed. The top end of the fixed column is rotatably installed with a culture bag box. A vacuum pump is installed on the right side of the upper end of the high platform, and a control panel is installed on the side of the high platform.
[0006] The culture bag box is of a hollow structure. At the four corners of the upper end surface of the culture bag box, buckles are arranged, and the buckles are connected in cooperation with the openings at the four corners of the culture bag.
[0007] At the two right corners of the bottom of the culture bag box, arc-shaped grooves are provided, and the arc-shaped grooves are magnetized. When the culture bag box is placed flat, the arc-shaped grooves are connected in cooperation with the magnetic columns respectively installed at the two right corners of the upper end of the low platform.
[0008] An arc-shaped groove is provided at the upper corner position of the left end face of the culture bag box. The arc-shaped groove is magnetized. When the culture bag box is vertical, the arc-shaped groove is cooperatively connected with two magnetic columns installed on the left side of the fixed column.
[0009] A battery is installed inside the high platform.
[0010] The control panel is electrically connected to the vacuum pump.
[0011] Before using the automatic liquid supplementing and pouring device for cell culture bags, first clean the device comprehensively and carefully disinfect it with alcohol to ensure that the device reaches a sterile state. After disinfection, place the device stably on the ultra-clean workbench, turn on the ultraviolet lamp of the ultra-clean bench, and irradiate for 30 minutes to achieve a sufficient disinfection effect and minimize the pollution risk during the operation process.
[0012] After the preparatory work is completed, place the culture medium bottle on the high platform, put the cell culture bag into the culture bag box, and firmly fix it with the buckles at the four corners to prevent the cell culture bag from moving during the operation.
[0013] During automatic liquid supplementing, make the culture bag box lie flat so that it is correspondingly adsorbed to the magnetic columns installed at the upper right corners of the low platform. Extend the hose of the cell culture bag from the hole of the culture bag box, carefully open the hose lid, put on a disposable sterile straw, insert the other end of the straw into the vacuum pump, and put the other outlet of the vacuum pump into the culture medium bottle through a sterile straw. Then press the power switch on the control panel to turn on the machine, and then press the liquid suction switch to perform vacuum liquid suction. Closely observe the liquid supplementing situation. When the required liquid supplementing amount is reached, press the power switch in time to stop the operation.
[0014] During automatic liquid pouring, carefully lift the culture bag box to make it vertical. After the cell suspension gathers at the end of the hose under the action of gravity, open the hose lid to pour the liquid, and pay attention to controlling the pouring speed to avoid liquid splashing.
[0015] After the operation is completed, put the culture bag back into the incubator and carefully clean the ultra-clean workbench to prepare for the next use.
[0016] Compared with the prior art, the beneficial effects of the present utility model are:
[0017] It is small and compact in design, does not occupy space, is especially suitable for use in an ultra-clean workbench, will not affect the operation space due to its too large volume, has a simple structure, and has no complex exposed wiring devices on the outside, reducing the risk of unnecessary pollution when used in the ultra-clean bench.
[0018] The culture medium bottle is placed on a high platform, and the culture bag box is placed with culture bags, and they have the same height, which can ensure that the cell suspension will not flow back into the culture medium bottle during vacuum liquid suction, avoiding culture medium contamination. Pouring liquid can be completed by using the action of gravity, without the need to hold or squeeze with both hands, reducing the labor intensity, ensuring that the cells are not damaged and have high activity.
[0019] The liquid replenishment method of vacuum pump type automatic liquid suction can minimize the air in the cell culture bag. Brief Description of the Drawings
[0020] Figure 1 It is a three-dimensional view of the main structure in the liquid replenishment state of an automatic liquid replenishment and pouring device for a cell culture bag of the present utility model;
[0021] Figure 2 It is a three-dimensional view of the main structure in the pouring state of an automatic liquid replenishment and pouring device for a cell culture bag of the present utility model;
[0022] In the figure: 11, culture bag box; 12, buckle; 21, high platform; 22, low platform; 23, fixed column; 24, magnetic column; 25, vacuum pump; 31, control panel. Specific Implementation Method
[0023] Next, the technical solutions in the embodiments of the present utility model will be fully described in conjunction with the accompanying drawings in the embodiments of the present utility model.
[0024] As Figure 1-2 shown, an automatic liquid replenishment and pouring device for a cell culture bag includes a high platform 21 and a low platform 22 fixedly installed at the bottom right of it. On both sides of the left position at the upper end of the low platform 22, two fixed columns 23 are respectively fixedly installed. On the left side of the fixed column 23 and at the two right corners of the upper end of the low platform 22, 4 magnetic columns 24 are respectively installed. The top of the fixed column 23 is rotatably installed with a culture bag box 11. On the right side of the upper end of the high platform 21, a vacuum pump 25 is installed, and on the side end of the high platform 21, a control panel 31 is installed.
[0025] The culture bag box 11 is of a hollow structure. At the four corners of the upper end surface of the culture bag box 11, buckles 12 are respectively arranged, and the buckles 12 are connected in cooperation with the openings at the four corners of the culture bag.
[0026] At the two right corners of the bottom of the culture bag box 11, arc-shaped grooves are provided, and the arc-shaped grooves are magnetized. When the culture bag box 11 is placed flat, the arc-shaped grooves are connected in cooperation with the magnetic columns 24 respectively installed at the two right corners of the upper end of the low platform 22.
[0027] An arc-shaped groove is provided at the upper corner position of the left end face of the culture bag box 11. The arc-shaped groove is magnetized. When the culture bag box 11 is vertical, the arc-shaped groove is cooperatively connected with two magnetic columns 24 installed on the left side of the fixed column 23.
[0028] A battery is installed inside the high platform 21.
[0029] The control panel 31 is electrically connected to the vacuum pump 25.
[0030] Before using the automatic liquid supplementing and pouring device for cell culture bags, first clean the device comprehensively and carefully disinfect it with alcohol to ensure that the device reaches a sterile state. After disinfection, place the device stably on the ultra-clean workbench, turn on the ultraviolet lamp of the ultra-clean bench, and irradiate for 30 minutes to achieve a sufficient disinfection effect and minimize the pollution risk during the operation process.
[0031] After the preparatory work is completed, place the culture medium bottle on the high platform 21, put the cell culture bag into the culture bag box 11, and firmly fix it with the buckles 12 at the four corners to prevent the cell culture bag from moving during the operation.
[0032] During automatic liquid supplementing, place the culture bag box 11 flat so that it is correspondingly adsorbed with the magnetic columns 24 installed at the two upper right corners of the upper end of the low platform 22. Extend the hose of the cell culture bag from the hole in the culture bag box 11, carefully open the hose lid, put on a disposable sterile straw, insert the other end of the straw into the vacuum pump 25, and put the other outlet of the vacuum pump 25 into the culture medium bottle through a sterile straw. Then press the power switch on the control panel 31 to turn on the machine, and then press the liquid suction switch to perform vacuum liquid suction. Closely observe the liquid supplementing situation. When the required liquid supplementing amount is reached, press the power switch in time to stop the operation.
[0033] During automatic pouring, carefully lift the culture bag box 11 to make it vertical. After the cell suspension gathers at the end of the hose port under the action of gravity, open the hose lid to achieve pouring, and pay attention to controlling the pouring speed to avoid liquid splashing.
[0034] After the operation is completed, put the culture bag back into the incubator and carefully clean the ultra-clean workbench to prepare for the next use.
Claims
1. An automatic liquid replenishment and pouring device for a cell culture bag, comprising a high platform (21) and a low platform (22) fixedly mounted on the right bottom, characterized in that Two fixed columns (23) are fixedly installed on both sides of the left upper end of the low platform (22), four magnetic columns (24) are installed on the left side of the fixed columns (23) and the right corners of the upper end of the low platform (22), a culture bag box (11) is rotatably installed on the top of the fixed column (23), a vacuum pump (25) is installed on the right side of the upper end of the high platform (21), and a control panel (31) is installed on the side end of the high platform (21).
2. The automatic liquid replenishing and pouring device for a cell culture bag according to claim 1, characterized in that: The culture bag box (11) is a hollow structure, and buckles (12) are arranged at the four corners of the upper end surface of the culture bag box (11), and the buckles (12) are matched and connected with the openings at the four corners of the culture bag.
3. The automatic liquid replenishing and pouring device for a cell culture bag according to claim 1, characterized in that: The bottom right corners of the culture bag box (11) are provided with arc-shaped grooves, and the arc-shaped grooves are magnetized. When the culture bag box (11) is laid flat, the arc-shaped grooves are connected with magnetic columns (24) respectively installed at the right corners of the upper end of the low platform (22).
4. The automatic liquid replenishing and pouring device for a cell culture bag according to claim 1, characterized in that: A circular arc groove is arranged at the upper corner of the left end surface of the culture bag box (11), and the circular arc groove is magnetized. When the culture bag box (11) is vertical, the circular arc groove is connected with two magnetic columns (24) installed on the left side of the fixing column (23).
5. The automatic liquid replenishing and pouring device for a cell culture bag according to claim 1, characterized in that: A battery is installed inside the high platform (21).
6. The automatic liquid replenishing and pouring device for a cell culture bag according to claim 1, characterized in that: The control panel (31) is electrically connected to the vacuum pump (25).