Detachable row small column suitable for purifying pesticide residues of tea leaves

Abstract

The utility model discloses a detachable row small column suitable for tea pesticide residue purification, which comprises a row purification column, a linkage pressing type piston is inserted in the row purification column, and the row purification column is filled with an adsorption layer located at the bottom of the linkage pressing type piston. A multi-position sample base plate is arranged at the bottom of the row purification column, a sample collection bottle is stored in the multi-position sample base plate, the row purification column is communicated with the sample collection bottle, a spiral bottle sleeve is movably connected to the bottom of the row purification column, and the spiral bottle sleeve is arranged on the surface of the sample collection bottle in a sleeving mode. The sample collection bottle is in threaded connection with the spiral bottle sleeve, the row purification column is composed of five single needle type filter columns in a row, and the top of the row purification column is provided with a needle cylinder piston matching feed port. According to the utility model, the elution time is greatly shortened, the working efficiency of traditional single sample / single purification is improved to (2-5) sample / single purification, and the working efficiency is improved by 2-5 times.

Description

Technical Field

[0001] The utility model relates to the technical field of tea pesticide residue purification, in particular to a detachable row-connected small column suitable for tea pesticide residue purification. Background Technique

[0002] All along, China has been a big tea-producing and -consuming country. As an important primary agricultural product in China, its safety has attracted more and more attention. However, due to the irregular use of pesticides in the planting process, the problem of tea pesticide residues cannot be directly avoided, and the situation of excessive tea pesticide residues still occurs, which has an adverse impact on tea consumption and exports.

[0003] However, due to the deficiencies in the existing purification of complex tea matrices, the tea composition is complex, rich in compounds such as alkaloids, tea polyphenols, caffeine, chlorophyll, sugars, and amino acids, which severely restricts the high-throughput determination of trace tea pesticide residues.

[0004] Therefore, it is necessary to design and transform a detachable row-connected small column suitable for tea pesticide residue purification. Content of the Utility Model

[0005] To solve the problems raised in the above background technique, the purpose of the present utility model is to provide a detachable row-connected small column suitable for tea pesticide residue purification, which has the advantage of improving the detection accuracy, and solves the deficiencies in the existing purification of complex tea matrices. The tea composition is complex, rich in compounds such as alkaloids, tea polyphenols, caffeine, chlorophyll, sugars, and amino acids, which severely restricts the high-throughput determination of trace tea pesticide residues.

[0006] To achieve the above purpose, the present utility model provides the following technical solution: A detachable row-connected small column suitable for tea pesticide residue purification, including a row-connected purification column;

[0007] A linkage pressing piston is inserted into the row-connected purification column. An adsorption layer is filled inside the row-connected purification column at the bottom of the linkage pressing piston. A multi-position sample chassis is arranged at the bottom of the row-connected purification column. A sample collection bottle is stored inside the multi-position sample chassis. The row-connected purification column is in communication with the sample collection bottle. A spiral bottle sleeve is movably connected to the bottom of the row-connected purification column. The spiral bottle sleeve is sleeved on the surface of the sample collection bottle. The sample collection bottle is threadedly connected to the spiral bottle sleeve.

[0008] Preferably, the row-connected purification column is composed of five single needle-type filter columns connected in a row. A syringe piston matching feeding port is arranged at the top of the row-connected purification column.

[0009] Preferably, the linkage pressing piston of the present utility model includes a finger-type extrusion head. A bearing plate is fixedly connected to the bottom of the finger-type extrusion head. The side of the bearing plate away from the finger-type extrusion head extends into the inside of the row-connected purification column. A rubber piston located inside the row-connected purification column is fixedly connected to the bottom of the bearing plate. A U-shaped detachable card sleeve is slidably connected to the top of the finger-type extrusion head.

[0010] Preferably, the overall height of the adsorption layer is 2.5 - 3.5 cm. The adsorption layer includes a hydrophobic sieve plate filled inside the row-connected purification column, and a filler is filled inside the hydrophobic sieve plate.

[0011] Preferably, the pore diameter of the hydrophobic sieve plate is 20 - 50 μm, and the filler is a mixture of anhydrous magnesium sulfate, ethylenediamine-N-propylsilane (PSA), octadecylsilane-bonded silica gel (C 18 ), and amino-functionalized multi-walled carbon nanotubes.

[0012] Preferably, the masses of anhydrous magnesium sulfate, ethylenediamine-N-propylsilane (PSA), octadecylsilane-bonded silica gel (C 18 ), and amino-functionalized multi-walled carbon nanotubes are 250 mg, 50 mg, 30 mg, and 70 mg respectively. The particle size range of ethylenediamino-N-propyl is 40 - 60 μm, and the particle size of octadecylsilane-bonded silica gel (C 18 ) is 40 - 60 μm. The particle size range of amino-functionalized multi-walled carbon nanotubes is 8 - 15 nm.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] 1. The present utility model greatly shortens the elution time, improving the working efficiency from the traditional single-sample / single-time purification to (2 - 5) samples / single time, and the working efficiency is increased by 2 - 5 times.

[0015] 2. The present utility model realizes "purification + filtration" in one step, reduces the sample loss caused by the working process and multiple transfer processes, is fast, and can be used for batch subsequent on-machine determination. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic structural diagram of the present utility model;

[0017] Figure 2 is a schematic sectional structural diagram of the present utility model;

[0018] Figure 3 is a schematic partial structural diagram of the present utility model;

[0019] Figure 4 is a schematic partial sectional structural diagram of the present utility model.

[0020] In the figure: 1. Row-connected purification column; 2. Linkage pressing piston; 3. Adsorption layer; 4. Multi-sample chassis; 5. Sample collection bottle; 6. Screw bottle sleeve; 7. Syringe piston matching feeding port; 8. Finger-type extrusion head; 9. Bearing plate; 10. Rubber piston; 11. U-shaped detachable bushing; 12. Hydrophobic sieve plate; 13. Packing material. Specific implementation manner

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] As Figures 1 to 4 shown, a detachable row-connected small column applicable to the purification of tea pesticide residues provided by the present invention includes a row-connected purification column 1;

[0023] A linkage pressing piston 2 is inserted into the row-connected purification column 1. An adsorption layer 3 is filled inside the row-connected purification column 1 at the bottom of the linkage pressing piston 2. A multi-sample chassis 4 is arranged at the bottom of the row-connected purification column 1. A sample collection bottle 5 is stored inside the multi-sample chassis 4. The row-connected purification column 1 is in communication with the sample collection bottle 5. A screw bottle sleeve 6 is movably connected to the bottom of the row-connected purification column 1. The screw bottle sleeve 6 is sleeved on the surface of the sample collection bottle 5. The sample collection bottle 5 is threadedly connected to the screw bottle sleeve 6.

[0024] Referring to Figure 2 , the row-connected purification column 1 is composed of five single needle-type filter columns connected in a row. A syringe piston matching feeding port 7 is arranged at the top of the row-connected purification column 1.

[0025] Referring to Figure 4 , the linkage pressing piston 2 includes a finger-type extrusion head 8. A bearing plate 9 is fixedly connected to the bottom of the finger-type extrusion head 8. One side of the bearing plate 9 away from the finger-type extrusion head 8 extends into the row-connected purification column 1. A rubber piston 10 located inside the row-connected purification column 1 is fixedly connected to the bottom of the bearing plate 9. A U-shaped detachable bushing 11 is slidably connected to the top of the finger-type extrusion head 8.

[0026] Referring to Figure 4 , the overall height of the adsorption layer 3 is 2.5 - 3.5 cm. The adsorption layer 3 includes a hydrophobic sieve plate 12 filled inside the row-connected purification column 1. The packing material 13 is filled inside the hydrophobic sieve plate 12.

[0027] Referring to Figure 4, the pore diameter of the hydrophobic sieve plate 12 is 20 - 50 μm, and the packing 13 is a mixture of anhydrous magnesium sulfate, ethylenediamine - N - propylsilane PSA, octadecylsilane - bonded silica gel C 18 , and amino - functionalized multi - walled carbon nanotubes.

[0028] Reference Figure 4 , the masses of anhydrous magnesium sulfate, ethylenediamine - N - propylsilane PSA, octadecylsilane - bonded silica gel C 18 , and amino - functionalized multi - walled carbon nanotubes are 250 mg, 50 mg, 30 mg, and 70 mg respectively. The particle size range of ethylenediamino - N - propyl is 40 - 60 μm, and that of octadecylsilane - bonded silica gel C 18 : 40 - 60 μm, and the particle size range of amino - functionalized multi - walled carbon nanotubes is 8 - 15 nm.

[0029] The working principle and usage process of the present utility model: Select 5 tea samples of 500 g each. After pulverizing and fully mixing them, use them as test samples. Weigh 1 g of the sample accurately to 0.01 g into a 50 mL plastic centrifuge tube, add 10 mL of water, vortex - mix for 1 min, and let it stand for 30 min. Add 10 mL of acetonitrile - acetic acid solution V:V = 99:1, then add 6 g of anhydrous magnesium sulfate, 1.5 g of sodium acetate, and 1 ceramic homogenizer. After vigorous shaking, perform ultrasonic treatment for 15 min, and then centrifuge at 6000 r / min for 5 min. For the above 5 samples, respectively pipette 2 mL of the supernatant into a row - type purification column 1 pre - fixed with a U - type detachable ferrule 11 and containing 250 mg of anhydrous magnesium sulfate, 50 mg of ethylenediamine - N - propylsilane PSA, 30 mg of octadecylsilane - bonded silica gel C 18 , and 70 mg of amino - functionalized multi - walled carbon nanotubes. The lower ends of the row - type purification columns 1 are respectively connected to sample collection bottles 5 placed on a multi - position sample chassis 4. After all the above supernatants have been transferred, use a linkage - pressed piston 2 to squeeze and collect the filtrate at a speed of 1 - 2 drops / second all at once. After collection, directly inject the sample solution into a GC - MS / MS instrument for analysis.

[0030] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non - exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0031] Although embodiments of the present utility model have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A detachable row of small columns suitable for purifying pesticide residues in tea leaves, comprising a row of purification columns (1); Features: The interior of the row purification column (1) is plugged with a linked push-type piston (2), and the interior of the row purification column (1) is filled with an adsorption layer (3) located at the bottom of the linked push-type piston (2). The bottom of the row purification column (1) is provided with a multi-position sample base (4), and the interior of the multi-position sample base (4) stores a sample collection bottle (5). The row purification column (1) and the sample collection bottle (5) are interconnected, and the bottom of the row purification column (1) is movably connected with a spiral bottle sleeve (6), and the spiral bottle sleeve (6) is sleeved on the surface of the sample collection bottle (5), and the sample collection bottle (5) is threadedly connected to the spiral bottle sleeve (6).

2. The detachable column for purifying pesticide residues in tea leaves according to claim 1, characterized in that: The row purification column (1) is composed of five single needle filter columns rowed together, and a syringe piston matching feeding port (7) is provided at the top of the row purification column (1).

3. The detachable column suitable for purifying pesticide residues in tea leaves according to claim 1, characterized in that: The linked pressing piston (2) comprises a finger-type extrusion head (8), the bottom of the finger-type extrusion head (8) is fixedly connected to a pressure plate (9), the pressure plate (9) extends to the interior of the row purification column (1) away from the side of the finger-type extrusion head (8), the bottom of the pressure plate (9) is fixedly connected to a rubber piston (10) located inside the row purification column (1), and the top of the finger-type extrusion head (8) is slidably connected to a U-shaped detachable sleeve (11).

4. The detachable column for purifying pesticide residues in tea leaves according to claim 1, characterized in that: The overall height of the adsorption layer (3) is 2.5 to 3.5 cm. The adsorption layer (3) comprises a hydrophobic sieve plate (12) filled inside the row purification column (1), and the inner side of the hydrophobic sieve plate (12) is filled with filler (13).

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