Multi-point fluorescence detection device

Abstract

The utility model provides a multi-point fluorescence detection device which comprises an optical module which is arranged at a position corresponding to an optical fiber matrix and is used for providing an excitation light signal for an optical fiber module; the optical fiber module receives an excitation light signal emitted by the optical module, transmits the excitation light signal to a plurality of to-be-detected areas, and transmits reflection signals generated by fluorescence reaction of the plurality of to-be-detected areas to the acquisition module; and the acquisition module is used for acquiring the reflected light signal and transmitting the reflected light signal to a remote terminal. The device further comprises a movement module which is used for driving the optical fiber module and the unit to be detected to move, so that the optical fiber module is matched with the optical module, and smooth transmission of optical signals is achieved. The optical fiber module disclosed by the utility model adopts a plurality of independent optical fiber bundles, so that detection signals of a plurality of irregularly distributed fluorescence detection areas in different areas can be independently transmitted, a plurality of irregularly distributed point positions can be simultaneously detected, and the working efficiency of detection is improved.

Description

Technical Field

[0001] The utility model relates to the field of fluorescence detection of biological reagents. Specifically, it relates to a fluorescence detection device applied to a device with multiple non-linear path detection points and a movable module. Background Art

[0002] Currently, most nucleic acid detection analyzers on the market (such as PCR instruments and isothermal amplification analyzers) have a pattern of single tubes, 8-well strip tubes, and 96-well plates as their supporting consumables. Therefore, the fluorescence detection schemes of these instruments require the sampling route of the fluorescence detection module to be a regular route. For some instruments that perform fluorescence detection on the side of the consumables (such as single tubes or 8-well strip tubes), the detection route must also be a single straight line path and cannot perform fluorescence detection along a curved path. Moreover, the detection position is mostly top detection, which requires the consumables to be inserted from the top of the instrument, which is not conducive to the stacking design of the instrument.

[0003] Currently, a large number of institutions in the market are engaged in the research, development, and sales of POCT test kits and supporting instruments. Various fluorescence reaction regions are designed on the test kits. However, due to the limitations of the existing fluorescence detection schemes, the test kits have to be designed as single fluorescence reaction regions (such as the GeneXpert tester of Cepheid Company and the Lifeready series analyzers of Lifereal), or multiple fluorescence reaction regions are designed on the same straight line (such as the UC series analyzers of Ustar Company and the QIAstat-DX® Analyzer of GIAGEN Company). Because if multiple fluorescence reaction regions are designed in a region with non-linear paths (such as a dish-shaped, cylindrical, or other test kits containing irregular curved path detection points), the fluorescence detection device in the instrument needs to perform multi-dimensional movements, which greatly increases the design difficulty and product cost of the instrument. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a fluorescence detection device applied to a device with multiple non-linear path detection points and a movable module.

[0005] To achieve the above purpose, the utility model adopts the following technical solutions:

[0006] The utility model provides a multi-point fluorescence detection device, including:

[0007] An optical module, arranged at a position corresponding to the optical fiber matrix, providing an excitation light signal for the optical fiber module;

[0008] The optical fiber module receives the excitation light signal emitted by the optical module, transmits it to multiple regions to be detected, and respectively transmits the reflection signals generated by the fluorescence reactions in the multiple regions to be detected to the acquisition module;

[0009] The acquisition module is used to acquire the reflected light signal and transmit it to the remote terminal.

[0010] Further, the optical fiber module includes a plurality of optical fiber bundles. One ends of the plurality of optical fiber bundles are integrated together to form an optical fiber matrix, and the optical fiber ends at the other ends are respectively arranged at positions corresponding to each fluorescence reaction region.

[0011] Further, each of the optical fiber bundles is independent of each other.

[0012] Further, the optical module includes a box body, a light source, a lens, a first filter, a second filter and a dichroic mirror. The inside of the box body is hollow. The lens is connected inside the box body and divides the inside of the box body into a first cavity and a second cavity. The light source is arranged on one side of the first cavity opposite to the lens. A dichroic mirror inclined at 45° is connected in the second cavity. The light emitted by the light source passes through the lens to form parallel light, which becomes downward excitation light under the refraction of the dichroic mirror; through holes for light to pass through are arranged above and below the second cavity, a second filter is arranged at the through hole above, and a first filter is arranged on the side of the lens close to the second cavity.

[0013] Further, the acquisition module includes an industrial camera 31 and a macro lens 32.

[0014] Further, the industrial camera captures an image of the reflected light signal and transmits it to the remote terminal by wired or wireless means, and the remote terminal analyzes and processes the image.

[0015] Further, a motion module is further included. The motion module is used to drive the optical fiber module and the area to be detected to move, so that the optical fiber module matches the optical module to realize the smooth transmission of the optical signal.

[0016] Further, the motion module includes a slide rail, a slider, a motion base and a driving device. The slider is connected to the motion base and moves on the slide rail along with the slider. The slider reciprocates on the slide rail under the drive of the driving device; one side of the motion base is fixedly connected with the optical fiber matrix, and a detection area is arranged on the other side. The light ends of each optical fiber bundle are respectively fixed at positions on the motion chassis corresponding to a fluorescence detection area.

[0017] Further, the driving device is connected to the slider through a belt drive structure.

[0018] The beneficial effect of the present utility model lies in that: the optical fiber module of the present utility model adopts a plurality of independent optical fiber bundles, realizing that the detection signals of multiple irregularly distributed fluorescence detection areas in different regions can be independently transmitted, enabling the simultaneous detection of multiple irregularly distributed points, and improving the detection work efficiency.

[0019] Meanwhile, different from most nucleic acid analyzers (PCR machines) or fully automatic nucleic acid analyzers (POCT) on the market, since the optical components, optical fiber components, and acquisition components are all in a fixed mode, the supporting consumables have to be inserted from the top of the instrument. Such a design is not conducive to the stacking design of the instrument. The present utility model adds a motion module, enabling the area to be detected on the device to extend out of the device for installing consumables to be detected (such as reagents, etc.), and during the motion process, it can ensure that the positions of the optical components, optical fiber components, and acquisition components basically do not change. In this way, not only can the installation method of the consumables be changed to side installation (instead of top installation), but also the performance of fluorescence acquisition can be ensured not to be affected. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic structural diagram of the fluorescence detection device of the present utility model;

[0021] Figure 2 is a schematic structural diagram of the optical module of the present utility model;

[0022] Figure 3 is a schematic structural diagram of a chassis to be detected that can be used in conjunction with the present utility model.

[0023] DESCRIPTION OF THE REFERENCE NUMERALS:

[0024] 11 optical fiber matrix, 12 optical fiber end, 13 optical fiber bundle, 21 box body, 22 light source, 23 lens, 24 first filter 25, second filter, 26 dichroic mirror, 27 first cavity, 28 second cavity, 31 industrial camera, 32 macro lens, 41 slide rail, 42 slider, 43 motion base, 44 belt drive structure, 5 detection chassis, 51 fluorescence detection area. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] The following will describe the exemplary embodiments of the present utility model in more detail with reference to the drawings. Although the exemplary embodiments of the present utility model are shown in the drawings, it should be understood that the present utility model can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided so that the present utility model can be more thoroughly understood and the scope of the present utility model can be completely conveyed to those skilled in the art.

[0026] This embodiment provides a fluorescence detection device applied to a fluorescence detection device including multiple non-linear path detection points and a movable module, including an optical fiber module, an optical module, an acquisition module, and a motion module:

[0027] Optical fiber module, the optical fiber module includes a plurality of optical fiber bundles 13, one ends of the plurality of optical fiber bundles are integrated together to form an optical fiber matrix 11, and the optical fiber ends 12 at the other ends are respectively fixed at positions corresponding to the respective fluorescence reaction regions on the chassis to be detected; since each optical fiber bundle can be independently installed (for example, on a moving base, it refers to the mounting holes corresponding to the optical fiber bundles and are fixed with tightening screws on the side), the optical fiber bundles are independent of each other and can be used for the independent transmission of the fluorescence signals of any irregularly distributed fluorescence reaction regions respectively.

[0028] The optical fiber module (optical fiber matrix end) receives the excitation light signal emitted by the optical module, transmits it to a plurality of regions to be detected, and respectively transmits the reflection signals generated by the fluorescence reactions of the plurality of regions to be detected to the acquisition module;

[0029] Optical module, arranged at a position corresponding to the optical fiber matrix, provides an excitation light signal for the optical fiber module,

[0030] The optical module includes a box body 21, a light source 22, a lens 23, a first filter 24, a second filter 25 and a dichroic mirror 26. The inside of the box body is hollow. The lens is connected inside the box body and divides the inside of the box body into a first cavity 27 and a second cavity 28. A light source is provided on one side of the first cavity opposite to the lens. A dichroic mirror arranged at a 45° inclination is connected in the second cavity. The light emitted by the light source passes through the lens to form parallel light and becomes downward excitation light under the refraction of the dichroic mirror; through holes for light to pass through are provided above and below the second cavity. A second filter is provided at the through hole above. A first filter is provided on the side of the lens close to the second cavity. When the device is in the detection state, the through hole below the second cavity is located above the optical fiber matrix; at this time, the excitation light can be transmitted to the optical fiber matrix through this through hole, and then transmitted to each fluorescence reflection area through each optical fiber bundle. The fluorescence reaction area generates a reflected light signal under the irradiation of the excitation light signal, and the reflected light signal is transmitted to the optical fiber matrix through the optical fiber bundle, and then passes through the dichroic mirror and the second filter to be transmitted to the acquisition module above.

[0031] The acquisition module is used to acquire the reflected light signal and transmit it to the remote terminal. The acquisition module includes an industrial camera 31 and a macro lens 32. The industrial camera captures an image of the reflected light signal and transmits it to the remote terminal in a wired or wireless manner. The remote terminal analyzes and processes the image. Since the acquisition module can simultaneously acquire the reflected light signals transmitted by a plurality of optical fiber bundles in the optical fiber matrix, the information acquisition efficiency is higher than that of the common single-point fluorescence detection mode.

[0032] Motion module, the motion module is used to drive the optical fiber module and the unit to be detected to move, so that the optical fiber module matches the optical module to ensure the smooth transmission of the optical signal.

[0033] The motion module includes a slide rail 41, a slider 42, a motion base 43 and a driving device. The slider is connected to the motion base and moves on the slide rail along with the slider. The slider reciprocates on the slide rail driven by the driving device. One side of the motion base is fixedly connected with an optical fiber matrix, and the other side is provided with a region to be detected. The light ends of each optical fiber bundle are respectively fixed at positions on the motion chassis corresponding to a fluorescence detection region.

[0034] The driving device can be a motor, and the motor can be connected to the slider through a belt drive structure.

[0035] The fluorescence detection region can be used in cooperation with the detection chassis described in Patent 202220682820.3, such as Figure 3 As shown, the detection chassis 5 includes a plurality of fluorescence detection regions 51.

[0036] During use, start the driving motor, the slider drives the motion base to move on the slide rail, so that the region to be detected moves outside the device. Place a plurality of samples to be detected in the fluorescence detection regions 51 respectively. Drive the motor to make the motion base move towards the inside of the device until the optical fiber matrix matches the through hole at the lower end of the optical module, ensuring the smooth transmission of optical signals. Turn on the light source, the fluorescence detection region undergoes a fluorescence reaction, take pictures with an industrial camera, and transmit the images to the remote terminal. At this time, since each optical fiber bundle can be independently installed (for example, on the motion base, it refers to the mounting holes corresponding to the optical fiber bundles and is fixed with locking screws on the side), each optical fiber bundle is independent of each other and can be used for the independent transmission of fluorescence signals in any irregularly distributed fluorescence reaction region. The acquisition module can simultaneously acquire the reflected optical signals transmitted by multiple optical fiber bundles in the optical fiber matrix, so the information acquisition efficiency is higher than the common single-point fluorescence detection mode.

[0037] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-point fluorescence detection device, characterized in that: include: An optical module is arranged at a position corresponding to the optical fiber matrix and provides an excitation light signal for the optical fiber module; The optical fiber module receives the excitation light signal emitted by the optical module and transmits it to a plurality of areas to be detected, and transmits the reflection signals generated by the fluorescence reaction of the plurality of areas to be detected to the collection module respectively; The acquisition module is used to collect reflected light signals and transmit them to the remote terminal.

2. The multi-point fluorescence detection device according to claim 1, characterized in that: The optical fiber module includes a plurality of optical fiber bundles, one end of which is integrated together to form an optical fiber matrix, and the optical fiber ends at the other end are respectively arranged at positions corresponding to each fluorescent reaction area.

3. The multi-point fluorescence detection device according to claim 2, characterized in that: The optical fiber bundles are independent of each other.

4. The multi-point fluorescence detection device according to claim 1, characterized in that: The optical module includes a box body, a light source, a lens, a first filter, a second filter and a dichroic mirror. The interior of the box body is hollow. The lens is connected to the interior of the box body and divides the interior of the box body into a first cavity and a second cavity. A light source is provided on the side of the first cavity opposite to the lens. A dichroic mirror is connected to the second cavity and is inclined at 45 degrees. Light emitted by the light source passes through the lens to form parallel light, which is refracted by the dichroic mirror and becomes downward excitation light. Through holes are provided above and below the second cavity for light to pass through. A second filter is provided at the upper through hole. A first filter is provided on the side of the lens close to the second cavity.

5. The multi-point fluorescence detection device according to claim 1, characterized in that: The acquisition module comprises an industrial camera (31) and a macro lens (32).

6. The multi-point fluorescence detection device according to claim 5, characterized in that: The industrial camera captures an image of the reflected light signal and transmits it to a remote terminal via a wired or wireless method, and the remote terminal analyzes and processes the image.

7. The multi-point fluorescence detection device according to any one of claims 1 to 6, characterized in that: It also includes a motion module, which is used to drive the optical fiber module and the unit to be detected to move, so that the optical fiber module matches the optical module and realizes smooth transmission of optical signals.

8. The multi-point fluorescence detection device according to claim 7, characterized in that: The motion module includes a slide rail, a slider, a motion base and a driving device. The slider is connected to the motion base and moves on the slide rail with the slider. The slider reciprocates on the slide rail driven by the driving device. One side of the motion base is fixedly connected to the optical fiber matrix, and the other side is provided with a detection area. The light end of each optical fiber bundle is respectively fixed at a position on the motion chassis corresponding to a fluorescent detection area.

9. The multi-point fluorescence detection device according to claim 8, characterized in that: The driving device is connected with the sliding block through a belt transmission structure.

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