Replacement cover assembly for cell rotary culture

Abstract

The utility model discloses a cell rotary culture replacement cover component, which is characterized in that the size of a cover plate is matched with that of a cell culture perforated plate, an outer shell covers the upper surface of the cover plate, a driving part is mounted on the outer shell, and mounting holes with the same number as hole sites are formed in the cover plate along the thickness direction of the cover plate. Each rotor comprises a connecting rod and a stirring part connected with the connecting rod, the stirring parts can be installed at the cell matrix support fixing sites, the connecting rods penetrate through the installing holes, the stirring parts are located on the lower surface of the cover plate, the transmission module is arranged in the outer shell, and the driving part is connected with the rotors through the transmission module to drive the multiple rotors to rotate synchronously. Through the design, the replacement cover assembly for the cell rotary culture in the embodiment can replace a top cover in a universal disposable cell culture perforated plate, so that a control experiment with synchronous rotating speed can be carried out, a cell matrix bracket can be arranged, the cost is low, and the operation is convenient. And the rotary cell culture experiment is carried out by the characteristics of convenience in cleaning and sterilization and small space occupation of the culture box.

Description

Technical Field

[0001] The utility model relates to the technical field of cell rotation culture, in particular to a cell rotation culture replacement cover assembly. Background Art

[0002] In traditional two-dimensional cell culture, cells usually grow on a flat culture medium. Although this method is simple and low-cost, its culture environment is very different from the three-dimensional growth environment of cells in vivo and cannot fully simulate the natural state of cells in vivo. Therefore, in order to better study cell behavior and drug effects, it is necessary to develop three-dimensional cell culture technologies that can simulate the in vivo environment.

[0003] Cell rotation culture technology is a type of three-dimensional cell culture technology. By adding structures such as rotors in a culture flask and continuously stirring the culture solution, cells can grow suspended in the culture solution, or shear force can be applied to the cells to simulate the in vivo body fluid flow environment. This technology can promote the interaction between cells, enhance the formation of the three-dimensional structure of cells, and better simulate the growth environment of cells in vivo, which is of great significance for the fields of cell biology, tissue engineering, and drug screening.

[0004] Existing cell rotation culture schemes usually use a magnetic stirrer to drive a magnetic rotor in a cell culture flask to achieve cell rotation culture. There are some disadvantages in such rotation culture methods.

[0005] (1) Traditional rotation culture flasks are generally large, which will waste a large amount of expensive reagents and cells for experiments with low cell number requirements.

[0006] (2) The space utilization efficiency of rotation culture flasks is low and they need to occupy a large amount of space in the cell culture incubator.

[0007] (3) When multiple groups of control experiments need to be carried out, it is difficult to control the rotational speed variables between groups.

[0008] (4) It cannot be applied to the currently emerging cell matrix scaffold technology, and traditional cell rotation culture schemes do not have cell matrix scaffold fixation sites.

[0009] Therefore, finding a technical solution that can solve the above technical problems has become an important research topic for those skilled in the art. Summary of the Utility Model

[0010] The embodiment of the utility model discloses a cell rotation culture replacement cover assembly for solving the above technical problems.

[0011] An embodiment of the present utility model provides a cell rotation culture replacement cover assembly for covering a cell culture multi-well plate. The cell culture multi-well plate is evenly distributed with a plurality of well positions for cell culture. The cell rotation culture replacement cover assembly includes an outer housing, a cover plate, a driving member, a transmission module, and a plurality of rotors;

[0012] The size of the cover plate matches that of the cell culture multi-well plate. The outer housing covers the upper surface of the cover plate. The driving member is installed on the outer housing. The cover plate is provided with installation holes having the same number as the well positions along its thickness direction. The positions of the plurality of installation holes correspond to the positions of the plurality of well positions one by one. The rotor includes a connecting rod and a stirring portion connected to the connecting rod. The connecting rod passes through the installation hole, and the stirring portion is located on the lower surface of the cover plate. The transmission module is disposed inside the outer housing. The driving member is connected to the rotor through the transmission module to drive the plurality of rotors to rotate synchronously.

[0013] Optionally, the transmission module includes a plurality of meshing transmission gears. The plurality of transmission gears are located inside the outer housing, and the positions of the plurality of rotors correspond to the positions of the plurality of transmission gears one by one. The connecting rod passes through the transmission gear and is fixedly connected to the transmission gear;

[0014] The output shaft of the driving member is connected to one of the transmission gears, or the output shaft of the driving member is connected to one of the connecting rods passing through the transmission gear;

[0015] The driving member drives one of the transmission gears to rotate to drive the other transmission gears to rotate synchronously, so that the plurality of rotors rotate synchronously, or the driving member drives one of the connecting rods passing through the transmission gear to rotate to drive the transmission gear to rotate, so that the plurality of rotors rotate synchronously.

[0016] Optionally, the transmission module further includes a plurality of ball bearings;

[0017] The plurality of ball bearings are disposed on the upper surface of the cover plate, and the positions of the plurality of ball bearings correspond to the positions of the plurality of transmission gears one by one. After the connecting rod passes through the upper surface of the cover plate, it is connected to the ball bearing.

[0018] Optionally, the transmission module further includes a connecting pipe;

[0019] The connecting pipe is located between the transmission gear and the ball bearing, and the upper end of the connecting pipe is connected to the transmission gear, and the lower end of the connecting pipe is connected to the ball bearing.

[0020] Optionally, the stirring portion is separated from the lower surface of the cover plate by a preset distance.

[0021] Optionally, a mounting seat for mounting the driving member is provided on the outer housing.

[0022] Optionally, the driving member is a servo motor.

[0023] From the above technical solutions, it can be seen that the embodiments of the present utility model have the following advantages:

[0024] The cell rotation culture replacement cover assembly in this embodiment can replace the top cover of a general disposable cell culture multi-well plate, which can avoid contamination of the culture system while ensuring gas exchange. When the cell rotation culture replacement cover assembly is covered on the cell culture multi-well plate, the driving member can make multiple rotors rotate synchronously through the transmission module, so as to stir the cell culture medium in multiple wells on the cell culture multi-well plate to make the cells grow suspended in the culture medium, thereby realizing cell rotation culture. In the above design, it is applicable to the cell rotation culture experimental device for a small-volume culture system, effectively saving reagents and cells, reducing the experimental cost, and greatly improving the space utilization rate without occupying a large amount of space in the incubator. Secondly, the driving member realizes the synchronous rotation of multiple rotors through the transmission module, effectively controlling the rotational speed variables between multiple groups of experiments, improving the flexibility and accuracy of the experiment, and meeting the demand for synchronous rotation culture of cells under multiple different culture conditions at the same rotational speed. In addition, through the above design, it can also provide fixed sites for cell matrix scaffolds, and can simulate the in-vivo matrix environment and the fluid shear force environment in the circulatory system. Description of the Drawings

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0026] Figure 1 It is a front structural perspective view of a cell rotation culture replacement cover assembly provided by an embodiment of the present utility model;

[0027] Figure 2 It is a side structural perspective view of a cell rotation culture replacement cover assembly provided by an embodiment of the present utility model;

[0028] Figure 3 It is a top structural perspective view of a cell rotation culture replacement cover assembly provided by an embodiment of the present utility model;

[0029] Figure 4 It is an exploded view of the structure of a cell rotation culture replacement cover assembly provided by an embodiment of the present utility model;

[0030] Illustration: Rotor 1; Stirring part 101; Connecting rod 102; Cover plate 2; Transmission gear 3; Ball bearing 4; Connecting pipe 5; Outer housing 6; Driving part 7. Specific implementation manners

[0031] To enable those skilled in the art to better understand the solution of the present utility model, the present utility model will be further described in detail below in conjunction with the accompanying drawings and specific implementation manners. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0032] Please refer to Figures 1 to 4 , a cell rotation culture replacement cover assembly provided by an embodiment of the present utility model is used to cover a cell culture multi-well plate. The cell culture multi-well plate is evenly distributed with a plurality of well positions for cell culture. The cell rotation culture replacement cover assembly includes an outer housing 6, a cover plate 2, a driving part 7, a transmission module, and a plurality of rotors 1;

[0033] The size of the cover plate 2 matches that of the cell culture multi-well plate. The outer housing 6 covers the upper surface of the cover plate 2. The driving part 7 is installed on the outer housing 6. The cover plate 2 is provided with mounting holes in the thickness direction thereof, and the number of the mounting holes is the same as the number of the well positions. The positions of the plurality of mounting holes correspond to the positions of the plurality of well positions one by one. The rotor 1 includes a connecting rod 102 and a stirring part 101 connected to the connecting rod 102. The connecting rod 102 passes through the mounting hole, and the stirring part 101 is located on the lower surface of the cover plate 2. The transmission module is arranged inside the outer housing 6. The driving part 7 is connected to the rotor 1 through the transmission module to drive the plurality of rotors 1 to rotate synchronously.

[0034] The stirring part 101 can be provided with fixed sites for cell matrix scaffolds, and can simultaneously simulate the circulating system environment of movement and the in-vivo cell matrix environment of rest.

[0035] It should be noted that the cell culture multi-well plate can be a 4-well plate, a 6-well plate, an 8-well plate, etc. In this embodiment, it is preferably a 6-well plate for cell culture. Therefore, the number of rotors 1 is also 6, so as to adapt to the 6 well positions of the 6-well plate for cell culture.

[0036] In addition, in order to enable the cell rotation culture replacement cover assembly to replace the top cover of a general disposable cell culture multi-well plate, the size of the cover plate 2 in this embodiment matches that of the cell culture multi-well plate, specifically reflected in the size and shape.

[0037] The cell rotation culture replacement cover assembly in this embodiment can replace the top cover of a general disposable cell culture multi-well plate. It can ensure gas exchange while avoiding contamination of the culture system. When the cell rotation culture replacement cover assembly is closed on the cell culture multi-well plate, the driving member 7 can make multiple rotors 1 rotate synchronously through the transmission module, so as to stir the cell culture medium in multiple wells on the cell culture multi-well plate to make the cells grow suspended in the culture medium, and then realize cell rotation culture. In the above design, it is applicable to the cell rotation culture experimental device for a small-volume culture system, effectively saving reagents and cells, reducing the experimental cost, and greatly improving the space utilization rate without occupying a large amount of space in the incubator. Secondly, the driving member 7 realizes the synchronous rotation of multiple rotors 1 through the transmission module, effectively controlling the rotational speed variables between multiple groups of experiments, improving the flexibility and accuracy of the experiment, and meeting the requirements of synchronous rotation culture of cells under multiple different culture conditions at the same rotational speed. In addition, through the above design, it can also provide fixed sites for cell matrix scaffolds, and can simulate the in-vivo matrix environment and the fluid shear force environment in the circulatory system.

[0038] Further, the transmission module in this embodiment specifically includes a plurality of meshing transmission gears 3. The plurality of transmission gears 3 are located inside the outer housing 6, and the positions of the plurality of rotors 1 correspond to the positions of the plurality of transmission gears 3 one by one. The connecting rod 102 passes through the transmission gear 3 and is fixedly connected to the transmission gear 3;

[0039] The output shaft of the driving member 7 is connected to one of the transmission gears 3, or the output shaft of the driving member 7 is connected to one of the connecting rods 102 passing through the transmission gear 3;

[0040] In the above design, when the driving member 7 drives one of the transmission gears 3 to rotate, it can drive the other transmission gears 3 to rotate synchronously, so as to make the plurality of rotors 1 rotate synchronously;

[0041] Alternatively, when the driving member 7 drives one of the connecting rods 102 passing through the transmission gear 3 to rotate, it can drive the transmission gear 3 to rotate, so as to make the plurality of rotors 1 rotate synchronously.

[0042] It should be noted that through the above design, the synchronous rotation of multiple rotors 1 can be realized, effectively controlling the rotational speed variables between multiple groups of experiments, improving the flexibility and accuracy of the experiment, and meeting different experimental requirements.

[0043] Further, the transmission module in this embodiment further includes a plurality of ball bearings 4;

[0044] A plurality of the ball bearings 4 are arranged on the upper surface of the cover plate 2, and the positions of the plurality of ball bearings 4 correspond to the positions of the plurality of transmission gears 3 one by one. After the connecting rod 102 passes through the upper surface of the cover plate 2, it is connected to the ball bearing 4.

[0045] It should be noted that through the above design, the rotor 1 can rotate more smoothly and steadily.

[0046] Furthermore, the transmission module in this embodiment further includes a connecting pipe 5;

[0047] The connecting pipe 5 is located between the transmission gear 3 and the ball bearing 4, and the upper end of the connecting pipe 5 is connected to the transmission gear 3, and the lower end of the connecting pipe 5 is connected to the ball bearing 4. Specifically, the lower end of the connecting pipe 5 is connected to the inner wall of the ball bearing 4;

[0048] It should be noted that through the above design, the connection between the ball bearing 4, the transmission gear 3 and the rotor 1 can be made more compact, ensuring the smoothness of the rotor 1 during rotation. In addition, the structure of the above transmission module has good sealing performance, effectively avoiding the contamination of the culture system and ensuring the accuracy and reliability of the experiment.

[0049] Furthermore, the stirring part 101 in this embodiment is separated from the lower surface of the cover plate 2 by a preset distance.

[0050] It should be noted that through the above design, during the rotation of the rotor 1, the interference between the stirring part 101 and the lower surface of the cover plate 2 can be avoided, thus affecting the smoothness of the rotation of the rotor 1.

[0051] Furthermore, the stirring part 101 in this embodiment is preferably of a trapezoidal structure. The above design provides a relatively stable flow environment for the culture system, which is beneficial to the growth and research of cells.

[0052] Furthermore, an installation seat for installing the driving member 7 is provided on the outer shell 6 in this embodiment.

[0053] It should be noted that through the above design, the driving member 7 can be stably installed on the outer shell 6.

[0054] Furthermore, in order to facilitate the control of the rotation speed of the rotor 1, the driving member 7 is preferably a servo motor. Specifically, the servo motor is a high-torque low-speed servo motor.

[0055] Furthermore, the outer shell 6 in this embodiment is of a square structure.

[0056] It should be noted that through the above design, the occupied space in the incubator can be further reduced, and at the same time, it is also beneficial to the flow of the culture solution, improving the uniformity of cell suspension culture.

[0057] Furthermore, the rotor 1 in this embodiment is preferably made of materials with relatively good biocompatibility such as stainless steel and titanium alloy;

[0058] The cover plate 2 and the connecting pipe 5 in this embodiment are preferably made of stainless steel or aluminum alloy to obtain better rigidity at a lower thickness;

[0059] The transmission gear 3 and the outer housing 6 in this embodiment are preferably made of nylon material to reduce the overall weight.

[0060] The above is a detailed description of the structure of a cell rotary culture replacement cover assembly provided by this embodiment. Next, a specific application process will be further described for it. The specific application process of a cell rotary culture replacement cover assembly in this embodiment is as follows:

[0061] After sterilizing the equipment, it is sent into the cell room laminar flow hood through the transfer window. After adding cells and the corresponding culture medium into a common plastic cell culture multi-well plate, cover the cell rotary culture replacement cover assembly, then transfer it into the cell carbon dioxide incubator, and then cover the equipment cover plate and connect the driving member 7. After turning on the power, cell rotary culture can be carried out.

[0062] Maintenance process: After each test, the cell rotary culture replacement cover assembly should be rinsed with clear water, ultrasonically cleaned and then cleaned 3 times each with double-distilled water and triple-distilled water to remove residual impurities. After drying, wrap it with tin foil and a sterilization bag, and then place it in an autoclave for sterilization treatment according to the solid sterilization procedure.

[0063] The above has introduced in detail a cell rotary culture replacement cover assembly provided by the present utility model. For those of ordinary skill in the art, based on the idea of the embodiments of the present utility model, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation to the present utility model.

Claims

1. A cell rotary culture replacement cover assembly, used to cover a cell culture multi-well plate, wherein the cell culture multi-well plate has a plurality of wells for cell culture evenly distributed thereon, characterized in that: The cell rotary culture replacement cover assembly comprises an outer shell, a cover plate, a driving member, a transmission module and a plurality of rotors; The size of the cover plate matches the cell culture multi-well plate, the outer shell is covered on the upper surface of the cover plate, the driving member is installed on the outer shell, the cover plate is provided with mounting holes with the same number as the hole positions along its thickness direction, the positions of the plurality of mounting holes correspond one-to-one with the positions of the plurality of hole positions, the rotor includes a connecting rod and a stirring part connected to the connecting rod, the connecting rod is passed through the mounting hole, and the stirring part is located on the lower surface of the cover plate, the transmission module is arranged inside the outer shell, and the driving member is connected to the rotor through the transmission module to drive the plurality of rotors to rotate synchronously.

2. The cell rotation culture replacement cover assembly according to claim 1, characterized in that: The transmission module comprises a plurality of mutually meshing transmission gears, the plurality of transmission gears are located in the outer shell, and the positions of the plurality of rotors correspond to the positions of the plurality of transmission gears one by one, and the connecting rod is passed through the transmission gears and fixedly connected to the transmission gears; The output shaft of the driving member is connected to one of the transmission gears, or the output shaft of the driving member is connected to one of the connecting rods passing through the transmission gear; The driving member drives one of the transmission gears to rotate to drive the other transmission gears to rotate synchronously, thereby causing the multiple rotors to rotate synchronously, or the driving member drives one of the connecting rods passing through the transmission gear to rotate to drive the transmission gear to rotate, thereby causing the multiple rotors to rotate synchronously.

3. The cell rotation culture replacement cover assembly according to claim 2, characterized in that: The transmission module also includes a plurality of ball bearings; The plurality of ball bearings are arranged on the upper surface of the cover plate, and the positions of the plurality of ball bearings correspond one-to-one to the positions of the plurality of transmission gears. The connecting rod passes through the upper surface of the cover plate and is connected to the ball bearings.

4. The cell rotation culture replacement cover assembly according to claim 3, characterized in that: The transmission module also includes a connecting pipe; The connecting pipe is located between the transmission gear and the ball bearing, and the upper end of the connecting pipe is connected to the transmission gear, and the lower end of the connecting pipe is connected to the ball bearing.

5. The cell rotation culture replacement cover assembly according to claim 1, characterized in that: The stirring portion is spaced apart from a lower surface of the cover plate by a preset distance.

6. The cell rotation culture replacement cover assembly according to claim 1, characterized in that: The driving component is a servo motor.

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