Ion trap fluorescence mass spectrometry detection composite device
By designing an ion trap fluorescence mass spectrometry detection composite device that integrates mass spectrometry detection components and fluorescence detection components, the problem that the prior art is difficult to provide both mass information and fluorescence information is solved, and multi-dimensional research and efficient detection of chemical reaction mechanisms are achieved.
Patent Information
- Application Number
- CN202421916103.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-08
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2034-08-08
AI Technical Summary
The existing ion trap technology is difficult to provide both mass information and fluorescence information, which limits in-depth research on the chemical reaction mechanism.
An ion trap fluorescence mass spectrometry detection composite device was designed, integrating linear ion trap, mass spectrometry detection components and fluorescence detection components to realize real-time fluorescence monitoring and mass spectrometry analysis of ions in the ion trap.
The device can detect the morphology and fluorescence dynamic changes of ions in real time, and provides multi-dimensional information through mass spectrometry analysis, which significantly improves the detection efficiency and comprehensiveness of information and promotes the study of chemical reaction mechanisms.
Smart Images
Figure CN222994322U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of measurement, and particularly relates to a composite device for ion trap fluorescence mass spectrometry detection. Background Art
[0002] Chemical reactions involve multiple reactants, intermediates, transition states, and products, and the process is complex and variable. In order to achieve an in-depth understanding and accurate analysis of the chemical reaction mechanism, the detection of multi-dimensional information is required. The ion trap technology is a commonly used technology for studying chemical reaction mechanisms. However, the existing ion trap technology has limited detection means and usually difficult to provide multi-dimensional data with both mass information and fluorescence information, which restricts the research on chemical reaction mechanisms. Summary of the Utility Model
[0003] The utility model aims to solve at least one of the technical problems existing in the prior art. For this purpose, the utility model provides a composite device for ion trap fluorescence mass spectrometry detection.
[0004] The composite device for ion trap fluorescence mass spectrometry detection according to an embodiment of the utility model includes a linear ion trap, a mass spectrometry detection component, and a fluorescence detection component. The mass spectrometry detection component is arranged beside the linear ion trap along the radial direction of the linear ion trap. A first slit for ion emission is formed on one side of the linear ion trap facing the mass spectrometry detection component, and the mass spectrometry detection component is used for mass spectrometry detection of the ions emitted from the first slit. The fluorescence detection component is arranged beside the linear ion trap, and the fluorescence detection component is used for fluorescence detection of multiple ions in the linear ion trap.
[0005] The composite device for ion trap fluorescence mass spectrometry detection according to an embodiment of the utility model has at least the following technical effects: By integrating the mass spectrometry detection component and the fluorescence detection component, real-time fluorescence monitoring and mass spectrometry analysis of ions are achieved in an ion trap device. This composite device can not only detect the morphology and fluorescence dynamic changes of ions in the linear ion trap in real time, but also control the ejection of ions by the linear ion trap and perform mass spectrometry analysis through the mass spectrometry detection component, thereby providing multi-dimensional information and greatly promoting the research on chemical reaction mechanisms. This method of obtaining dual information greatly improves the detection efficiency and comprehensiveness of information, enabling researchers to more accurately analyze the key steps and dynamic behaviors in the chemical reaction process.
[0006] According to some embodiments of the utility model, the mass spectrometry detection component includes an ion lens and a mass spectrometer. Taking the orientation of the mass spectrometry detection component relative to the linear ion trap as the right side, the mass spectrometer is arranged on the right side of the ion lens.
[0007] According to some embodiments of the present utility model, the number of the ion lenses is multiple, and the multiple ion lenses are arranged in sequence from left to right.
[0008] According to some embodiments of the present utility model, the ion lens includes a main body portion, a left shielding ring arranged on the left side of the main body portion, a right shielding ring arranged on the right side of the main body portion, and an outer shielding ring sleeved outside the main body portion.
[0009] According to some embodiments of the present utility model, the outer shielding ring has a left split ring and a right split ring, the left split ring and the left shielding ring are an integral member, and the right split ring and the right shielding ring are an integral member.
[0010] According to some embodiments of the present utility model, the left shielding ring, the right shielding ring, and the outer shielding ring are collectively referred to as a shielding component, and an insulating layer is connected between the shielding component and the main body portion.
[0011] According to some embodiments of the present utility model, the fluorescence detection component includes an objective lens, a beam splitter, a photon counter, and a camera. The objective lens is used to collect the fluorescence emitted by multiple ions in the linear ion trap, and the beam splitter is used to reflect a part of the fluorescence of the multiple ions collected by the objective lens to the photon counter and output the other part to the camera.
[0012] According to some embodiments of the present utility model, a filter is arranged between the beam splitter and the objective lens.
[0013] According to some embodiments of the present utility model, the number of the fluorescence detection components is two. One of the fluorescence detection components is arranged beside the linear ion trap along the radial direction of the linear ion trap, and is arranged beside the linear ion trap along the axial direction of the linear ion trap with the other fluorescence detection component.
[0014] According to some embodiments of the present utility model, the fluorescence detection component is arranged on a side of the linear ion trap away from the mass spectrometry detection component.
[0015] The additional aspects and advantages of the present utility model will be partially given in the following description, partially become obvious from the following description, or be understood through the practice of the present utility model. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The above and / or additional aspects and advantages of the present utility model will become obvious and easy to understand from the description of the embodiments in conjunction with the following drawings, where:
[0017] Figure 1 is a schematic perspective view of an ion trap fluorescence mass spectrometry detection composite device according to an embodiment of the present utility model;
[0018] Figure 2 It is a schematic structural diagram of an ion lens in an embodiment of the present utility model;
[0019] Figure 3 It is a schematic structural diagram of an ion trap fluorescence mass spectrometry detection composite device in an embodiment of the present utility model.
[0020] In the attached drawings:
[0021] 100 - linear ion trap; 111 - end electrode rod; 112 - radio frequency electrode rod; 200 - mass spectrometry detection component; 210 - ion lens; 211 - left shielding ring; 212 - left splitting ring; 213 - right shielding ring; 214 - right splitting ring; 215 - main body part; 220 - mass spectrometer detector; 300 - fluorescence detection component; 310 - objective lens; 320 - filter; 330 - dichroic mirror; 340 - camera; 350 - photon counter. Detailed implementation manners
[0022] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the attached drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below are only used to explain the present utility model and should not be construed as a limitation of the present utility model.
[0023] In the description of the present utility model, the orientation descriptions such as up, down, front, back, left, right, etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the attached drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating that the device or element referred to must have a specific orientation or operate in a specific orientation. Therefore, it should not be construed as a limitation of the present utility model. The meaning of several is one or more, the meaning of multiple is two or more, greater than, less than, exceeding, etc. are understood as not including the present number, and above, below, within, etc. are understood as including the present number. If there is a description of first and second, it is only for the purpose of distinguishing technical features and should not be understood as indicating relative importance or indicating the quantity of the indicated technical features or indicating the sequence relationship of the indicated technical features.
[0024] In the description of the present utility model, words such as setting, installation, connection, etc. should be understood in a broad sense. Those skilled in the art can reasonably determine the specific meanings of the above words in the present utility model in combination with the specific content of the technical solution.
[0025] The technical solutions of the present utility model will be described clearly and completely below with reference to the attached drawings. Obviously, the following described embodiments are some embodiments of the present utility model, not all embodiments.
[0026] The following refers to Figures 1 to 3 Describe an ion trap fluorescence mass spectrometry detection composite device according to an embodiment of the present utility model.
[0027] The ion trap fluorescence mass spectrometry detection composite device of the embodiment of the utility model includes a linear ion trap 100, a mass spectrometry detection component 200 and a fluorescence detection component 300. The mass spectrometry detection component 200 is arranged beside the linear ion trap 100 along the radial direction of the linear ion trap 100. A first slit for ion emission is arranged on the side of the linear ion trap 100 facing the mass spectrometry detection component 200. The mass spectrometry detection component 200 is used for mass spectrometry detection of the ions emitted from the first slit. The fluorescence detection component 300 is arranged beside the linear ion trap 100 and is used for fluorescence detection of a plurality of ions in the linear ion trap 100.
[0028] As Figure 1 shown, the fluorescence detection component 300, the linear ion trap 100 and the mass spectrometry detection component 200 are arranged in sequence from left to right. The linear ion trap 100 is used for capturing and trapping ions and includes four electrode rod assemblies. The four electrode rod assemblies all extend forward and backward. The four electrode rod assemblies are respectively located at the upper left side, upper right side, lower left side and lower right side of the linear ion trap 100. Each electrode rod assembly has two end electrode rods 111 and a radio frequency electrode rod 112 arranged between the two end electrode rods 111. The radio frequency electrode rod 112 and the two end electrode rods 111 are coaxial. The maximum outer diameters of the radio frequency electrode rod 112 and the two end electrode rods 111 are the same and the surfaces are polished. In order to ensure that the end electrode rod 111 and the radio frequency electrode rod 112 are not short-circuited and coaxial, they are tightly fitted to each other with insulating materials. The first slit is the slit between the radio frequency electrode rod 112 on the upper right side and the radio frequency electrode rod 112 on the lower right side. The slit between the radio frequency electrode rod 112 on the upper left side and the radio frequency electrode rod 112 on the lower left side is called the second slit, and the second slit faces the fluorescence detection component 300.
[0029] Among them, the linear ion trap 100 generates ions by bombarding the surface of the sample block with an electron gun or a laser. The ions enter the linear ion trap 100 from the bottom of the linear ion trap 100 and are trapped. When the linear ion trap 100 is used to trap ions, DC voltages are applied to both end electrode rods 111 of each electrode rod assembly. Among the four RF electrode rods 112, the one located in the upper left side and the one located in the lower right side form a pair, and the one located in the lower left side and the one located in the upper right side form a pair. One pair is applied with a DC voltage, and the other pair is applied with an AC voltage. When the linear ion trap 100 is used to eject ions for mass spectrometry detection, the voltages applied to the four RF electrode rods 112 change. The two RF electrode rods 112 far from the mass spectrometry detection component 200 are loaded to a high voltage value, called the first voltage value, within 150 nanoseconds. The two RF electrode rods 112 close to the mass spectrometry detection component 200 are synchronously loaded to a high voltage value, called the second voltage value. The second voltage value is less than the first voltage value. The typical value of the second voltage value is 1000V, and the typical value of the first voltage value is 1400V.
[0030] Compared with the existing technology, the electrode rods of the linear ion trap 100 in this embodiment are composed of cylindrical metal rods. The good arc curvature of the electrodes effectively ensures the uniform gradient distribution of the electric potential in space after each electrode rod is applied with an electric potential. At the same time, there is a gap of at least 5 mm between the electrode rod assemblies of the linear ion trap 100 in three dimensions, which is convenient for the light path to pass through when cooling the ions in the ion trap by laser in three-dimensional directions. In addition, the RF voltage and DC voltage on the RF electrode rod 112 can be rapidly increased to a high-voltage electric field of thousands of volts in the nanosecond level, which can effectively ensure that when the ions are rapidly ejected from the linear ion trap 100, the electric field force received by the ions is nearly collinear with the line connecting the center of the linear ion trap 100 and the center of the mass spectrometer detector 220, reducing the divergence angle when the ions are ejected from the linear ion trap 100.
[0031] The utility model provides an ion trap fluorescence mass spectrometry detection composite device, aiming to solve the limitations of the existing technology. By integrating the mass spectrometry detection component and the fluorescence detection component, the device realizes the simultaneous acquisition of real-time fluorescence monitoring and mass spectrometry analysis of ions in the ion trap device. This composite device can not only detect the morphology and fluorescence dynamic changes of ions in the linear ion trap in real time, but also control the ejection of ions from the linear ion trap and perform mass spectrometry analysis through the mass spectrometry detection component, thereby providing multi-dimensional information and greatly promoting the research on the chemical reaction mechanism. This method of obtaining dual information greatly improves the detection efficiency and the comprehensiveness of information, enabling researchers to more accurately analyze the key steps and dynamic behaviors in the chemical reaction process.
[0032] In some embodiments of the present utility model, the mass spectrometry detection assembly 200 includes an ion lens 210 and a mass spectrometry detector 220. Taking the orientation of the mass spectrometry detection assembly 200 relative to the linear ion trap 100 as the right side, the mass spectrometry detector 220 is arranged on the right side of the ion lens 210. The ion lens 210 is used to confine the divergent trajectories of ions in the direction perpendicular to the ion flight direction, so that ions of different masses can reach the position of the mass spectrometry detector 220, improving the detection accuracy. The mass spectrometry detector 220 includes one or more microchannel plates, a grounded electrode sheet and a detection sheet; alternatively, the mass spectrometry detector 220 includes one or more microchannel plates and a fluorescent screen, which is used for radially detecting ions. The mass spectrometry detector 220 amplifies the information of the ions and converts it into an electrical signal or a fluorescent signal for display. The center point of the mass spectrometry detector 220 is on the central axis of the ion lens 210. At the same time, the mass spectrometry detector 220 is kept at a distance of 300-500 mm from the linear ion trap 100. The appropriate distance between the mass spectrometry detector 220 and the linear ion trap 100 can ensure the resolution and sensitivity of the signal peaks detected by the mass spectrometry detector 220. A voltage of -1700 V is applied to the mass spectrometry detector 220 for signal amplification, and the signal line from the mass spectrometry detector 220 is connected to an oscilloscope for observing the signal.
[0033] In some embodiments of the present utility model, the number of ion lenses 210 is multiple, and the multiple ion lenses 210 are arranged in sequence from left to right. Further confine the divergent trajectories of ions in the direction perpendicular to the ion flight direction, so that ions of different masses can reach the position of the mass spectrometry detector 220, improving the detection accuracy.
[0034] In some embodiments of the present utility model, in order to shield the influence of the external electric field and improve the detection accuracy, the ion lens 210 includes a main body 215, a left shielding ring 211 arranged on the left side of the main body 215, a right shielding ring 213 arranged on the right side of the main body 215, and an outer shielding ring sleeved on the outside of the main body 215. The main body 215 is annular. After an appropriate voltage is applied to the main body 215, a confinement force can be provided in the direction perpendicular to the ion flight direction, reducing the divergence of ions in the direction perpendicular to the ion flight direction.
[0035] In some embodiments of the present utility model, with reference to Figure 2 , the outer shielding ring has a left split ring 212 and a right split ring 214. The left split ring 212 and the left shielding ring 211 are an integral component, and the right split ring 214 and the right shielding ring 213 are an integral component. Both the left shielding ring 211 and the right shielding ring 213 are made of metal materials; in this way, during assembly, only the main body 215 needs to be placed between the left shielding ring 211 and the right shielding ring 213, and then the left shielding ring 211 and the right shielding ring 213 are closed, which is convenient for production and assembly; moreover, during use, only the left shielding ring 211 and the right shielding ring 213 need to be grounded, and there is no need to ground the outer shielding ring separately, which is convenient for use.
[0036] In some embodiments of the present utility model, the left shielding ring 211, the right shielding ring 213 and the outer shielding ring are collectively referred to as the shielding component, and an insulating layer is connected between the shielding component and the main body 215. In this way, the connection between the shielding component and the main body 215 is compact and not easy to loosen.
[0037] In some embodiments of the present utility model, the fluorescence detection assembly 300 includes an objective lens 310, a beam splitter, a photon counter 350 and a camera 340. The objective lens 310 is used to collect the fluorescence emitted by multiple ions in the linear ion trap 100. The beam splitter is used to reflect a part of the fluorescence of the multiple ions collected by the objective lens 310 to the photon counter 350 and output the other part to the camera 340. The fluorescence detection assembly 300 of the present utility model can monitor the fluorescence intensity and ion morphology changes of the ions in the linear ion trap 100 in real time. By separating the fluorescence emitted by the ions through the beam splitter and recording it by the photon counter 350 and the camera 340, this device can accurately reflect the instantaneous change of the fluorescence intensity and provide information on the ion quantity and motion state. The camera 340 can also generate an ion fluorescence distribution image to realize the real-time observation of the ion morphology. Among them, the objective lens 310 can be a convex lens with a magnification of eight times, and the beam splitter can be a dichroic mirror 330.
[0038] The ion trap fluorescence mass spectrometry detection composite device can realize the real-time monitoring of ion fluorescence signals and combine with time-of-flight mass spectrometry analysis to provide an integrated solution for ion capture, storage and composition analysis. Through the application of this device, it is possible to efficiently and accurately monitor ion behavior and its composition, providing reliable technical support for related scientific research and applications.
[0039] In some embodiments of the present utility model, in order to filter the background light to improve the accuracy of fluorescence detection, a filter 320 is arranged between the beam splitter and the objective lens 310. The objective lens 310, the filter 320, the beam splitter and the camera 340 are arranged in sequence from right to left on the left side of the second slit. The beam splitter is arranged obliquely backward from right to left. The photon counter 350 is arranged behind the beam splitter, so that the light component passing through the beam splitter can be captured and collected by the camera 340, and the light component reflected by the beam splitter can be captured and collected by the photon counter 350.
[0040] In some embodiments of the present utility model, referring to Figure 3 , the number of the fluorescence detection assemblies 300 is two. One of the fluorescence detection assemblies 300 is arranged beside the linear ion trap 100 along the radial direction of the linear ion trap 100, and the other fluorescence detection assembly 300 is arranged beside the linear ion trap 100 along the axial direction of the linear ion trap 100. In this way, fluorescence detection can be carried out from multiple directions to obtain more fluorescence detection information, which is beneficial to the research on the chemical reaction mechanism.
[0041] In some embodiments of the present utility model, the fluorescence detection assembly 300 is disposed on the side of the linear ion trap 100 away from the mass spectrometry detection assembly 200. In this way, sufficient space is left for arranging the fluorescence detection assembly 300 and the mass spectrometry detection assembly 200 to avoid mutual interference.
[0042] The above has specifically described the preferred embodiments of the present utility model. However, the present utility model is not limited to the embodiments. Those skilled in the art can also make various equivalent variations or substitutions without departing from the spirit of the present utility model. These equivalent variations or substitutions are all included within the scope defined by the claims of this application.
Claims
1. An ion trap fluorescence mass spectrometry detection composite device, characterized in that: The invention comprises a linear ion trap, a mass spectrometer detection component and a fluorescence detection component. The mass spectrometer detection component is arranged at the side of the linear ion trap along the radial direction of the linear ion trap. A first slit for ion emission is provided on the side of the linear ion trap facing the mass spectrometer detection component. The mass spectrometer detection component is used to perform mass spectrometer detection on the ions emitted from the first slit. The fluorescence detection component is arranged at the side of the linear ion trap. The fluorescence detection component is used to perform fluorescence detection on multiple ions in the linear ion trap.
2. The ion trap fluorescence mass spectrometry detection composite device according to claim 1, characterized in that: The mass spectrometer detection component comprises an ion lens and a mass spectrometer detector. Taking the orientation of the mass spectrometer detection component relative to the linear ion trap as the right, the mass spectrometer detector is arranged on the right side of the ion lens.
3. The ion trap fluorescence mass spectrometry detection composite device according to claim 2, characterized in that: There are multiple ion lenses, and the multiple ion lenses are arranged in sequence from left to right.
4. The ion trap fluorescence mass spectrometry detection composite device according to claim 2, characterized in that: The ion lens comprises a main body, a left shielding ring arranged on the left side of the main body, a right shielding ring arranged on the right side of the main body, and an outer shielding ring sleeved on the outer side of the main body.
5. The ion trap fluorescence mass spectrometry detection composite device according to claim 4, characterized in that: The outer shielding ring comprises a left sub-ring and a right sub-ring, the left sub-ring and the left shielding ring are an integral component, and the right sub-ring and the right shielding ring are an integral component.
6. The ion trap fluorescence mass spectrometry detection composite device according to claim 4, characterized in that: The left shielding ring, the right shielding ring and the outer shielding ring are collectively referred to as a shielding component, and an insulating layer is connected between the shielding component and the main body.
7. The ion trap fluorescence mass spectrometry detection composite device according to claim 1, characterized in that: The fluorescence detection component includes an objective lens, a spectroscope, a photon counter and a camera. The objective lens is used to collect the fluorescence emitted by multiple ions in the linear ion trap, and the spectroscope is used to reflect part of the fluorescence of the multiple ions collected by the objective lens to the photon counter and output the other part to the camera.
8. The ion trap fluorescence mass spectrometry detection composite device according to claim 7, characterized in that: A filter is arranged between the beam splitter and the objective lens.
9. The ion trap fluorescence mass spectrometry detection composite device according to claim 1, characterized in that: There are two fluorescence detection components, one of which is arranged beside the linear ion trap along the radial direction of the linear ion trap, and the other of which is arranged beside the linear ion trap along the axial direction of the linear ion trap.
10. The ion trap fluorescence mass spectrometry detection composite device according to claim 1, characterized in that: The fluorescence detection component is arranged on a side of the linear ion trap away from the mass spectrometry detection component.