Protein separation equipment

Abstract

The utility model discloses protein separation equipment which comprises a separation tank, the top end of the separation tank is fixedly connected with a sealing cover, the inside of the sealing cover is rotatably connected with a rotating gear, the bottom end of the rotating gear is fixedly communicated with a separation barrel, and the top end of the sealing cover is fixedly connected with a mounting frame. The driving motor drives the driving gear to rotate, so that the rotating gear rotates along with the driving gear and drives the separation barrel to rotate, macromolecular protein in the separation barrel is separated, and the rotating gear drives the driven gear to rotate in the separation process, so that the reciprocating screw rod rotates to drive the scraping ring to slide up and down on the inner wall of the separation tank; the small-molecule protein and hydrops attached to the inner wall are rapidly gathered, the gathered mixed liquid rapidly falls into the bottom of the separation tank under the action of gravity to be collected, the recovery efficiency of the separation liquid is improved, small-molecule protein separation operation can be conveniently and rapidly carried out, and the overall efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of separation equipment, in particular to a protein separation equipment. Background Technique

[0002] Protein is a biological macromolecule polymerized by many amino acids. The types of proteins vary depending on the types and quantities of amino acids. Some proteins have biological catalytic functions or biological adsorption functions due to their special spatial structures and amino acid side chains. These proteins are called active proteins. When separating macromolecular proteins, centrifugation is usually used for rapid separation. Small molecular proteins in the separated mixture usually need to be separated again.

[0003] Currently, during the separation of macromolecular proteins, the separated mixture usually adheres to the inner surface of the separation tank due to centrifugal force and slowly flows along the inner wall under the action of gravity and converges, and finally flows out of the separation tank for collection. However, this method has a slow flow rate and requires more time, resulting in a low collection efficiency of the mixture. Content of the Utility Model

[0004] The purpose of the utility model is to provide a protein separation equipment to solve the problems raised in the above background technique.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A protein separation equipment, including a separation tank, a sealing cover is fixedly connected to the top of the separation tank, a rotating gear is rotatably connected inside the sealing cover, a separation barrel is fixedly communicated with the bottom end of the rotating gear, an installation frame is fixedly connected to the top of the sealing cover, a driving motor is fixedly connected to one side of the installation frame, the output end of the driving motor is drivingly connected with a driving gear, the outer surface of the driving gear is meshed with the outer surface of the rotating gear, two driven gears are rotatably connected to the top of the sealing cover, the outer surfaces of the two driven gears are both meshed with the outer surface of the rotating gear, the bottom ends of the two driven gears both penetrate through the top of the sealing cover and are drivingly connected with reciprocating lead screws, a scraping ring is meshed with the outer surfaces of the two reciprocating lead screws, and the outer surface of the scraping ring is closely attached to the inner surface of the separation tank.

[0006] As a further preferred of this technical solution, a sleeve is sleeved inside the separation barrel, a pressing cover is threadedly connected inside the installation frame, a connecting column is fixedly connected to the bottom end of the pressing cover, a sealing plate is rotatably connected to the bottom end of the connecting column, and the bottom end of the sealing plate is in close contact with the top end of the sleeve.

[0007] As a further preferred of this technical solution, two positioning grooves are opened on the inner surface of the separation barrel, two positioning rods are fixedly connected to the outer surface of the sleeve, and the outer surfaces of the two positioning rods are respectively slidably connected with the inner surfaces of the positioning grooves.

[0008] As a further preferred embodiment of the present technical solution, the bottom end of the sealing cover is fixedly connected to a stabilizing frame, and the bottom end of the separation barrel is rotatably connected to the inner bottom surface of the stabilizing frame.

[0009] As a further preferred embodiment of the present technical solution, two fixing seats are fixedly connected to the inner surface of the separation tank, and the bottom ends of the two reciprocating screw rods are rotatably connected to the inside of the two fixing seats respectively.

[0010] As a further preferred embodiment of the present technical solution, the bottom end of the separation tank is fixedly connected to a feed pipe, and the top end of the clamping cover is fixedly connected to a handle.

[0011] As a further preferred embodiment of the present technical solution, a handle is fixedly connected to the top end of the sleeve, and the outer surface of the sleeve is interference fit with the inner surface of the separation barrel.

[0012] The utility model provides a protein separation device, which has the following beneficial effects:

[0013] (1) The utility model drives the driving motor to rotate the active gear, so that the rotating gear follows the rotation and drives the separation barrel to rotate, so as to separate the large molecular protein in the separation barrel. During the separation process, the rotating gear drives the driven gear to rotate, so that the reciprocating screw rotates and drives the scraper ring to slide up and down on the inner wall of the separation tank, and the small molecular protein attached to the inner wall and the accumulated liquid are quickly gathered. The mixed liquid after gathering quickly falls to the bottom of the separation tank under the action of gravity for collection, thereby improving the recovery efficiency of the separation liquid, facilitating and quickly performing the small molecular protein separation operation, and improving the overall efficiency.

[0014] (2) The utility model rotates the compression cover to disengage it from the mounting frame and then remove it together with the sealing plate. Then, the sleeve is pulled out from the separation barrel by pulling the handle, and the next sleeve containing the raw materials to be separated is inserted into the separation barrel, thereby realizing rapid material replacement and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0016] Figure 2 It is a schematic diagram of the internal structure of the utility model as a whole;

[0017] Figure 3 It is a schematic diagram of the structure between the rotating gear and the scraper ring of the utility model;

[0018] Figure 4 It is a schematic diagram of the internal structure of the separation barrel of the utility model and its decomposition.

[0019] In the figure: 1, separation tank; 2, sealing cover; 3, rotating gear; 4, separation barrel; 5, mounting bracket; 6, drive motor; 7, driving gear; 8, driven gear; 9, reciprocating lead screw; 10, fixed seat; 11, scraping ring; 12, stabilizing frame; 13, discharge pipe; 14, sleeve; 15, positioning groove; 16, positioning rod; 17, handle; 18, pressing cover; 19, connecting column; 20, sealing plate; 21, grip. Detailed implementation manner

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention.

[0021] The present invention provides a technical solution: As Figures 1-4 shown, in this embodiment, a protein separation device includes a separation tank 1, a sealing cover 2 is fixedly connected to the top end of the separation tank 1, a rotating gear 3 is rotatably connected inside the sealing cover 2, a separation barrel 4 is fixedly communicated with the bottom end of the rotating gear 3, a mounting bracket 5 is fixedly connected to the top end of the sealing cover 2, a drive motor 6 is fixedly connected to one side of the mounting bracket 5, the output end of the drive motor 6 is drivingly connected with a driving gear 7, the outer surface of the driving gear 7 meshes with the outer surface of the rotating gear 3, two driven gears 8 are rotatably connected to the top end of the sealing cover 2, the outer surfaces of the two driven gears 8 both mesh with the outer surface of the rotating gear 3, the bottom ends of the two driven gears 8 both penetrate through the top end of the sealing cover 2 and are drivingly connected with a reciprocating lead screw 9, a scraping ring 11 meshes with the outer surfaces of the two reciprocating lead screws 9, and the outer surface of the scraping ring 11 is in close contact with the inner surface of the separation tank 1. Among them, by setting the driven gear 8, the reciprocating lead screw 9 can be driven to rotate during the rotation of the rotating gear 3 to achieve a speed reduction driving effect, and by setting the reciprocating lead screw 9, the up-and-down reciprocating movement of the scraping ring 11 can be realized.

[0022] As Figure 4 shown, a sleeve 14 is sleeved inside the separation barrel 4, a pressing cover 18 is threadedly connected inside the mounting bracket 5, a connecting column 19 is fixedly connected to the bottom end of the pressing cover 18, a sealing plate 20 is rotatably connected to the bottom end of the connecting column 19, and the bottom end of the sealing plate 20 is in close contact with the top end of the sleeve 14. By setting the sealing plate 20, the separation barrel 4 and the sleeve 14 can be sealed to prevent the material from splashing during the separation process.

[0023] As Figure 4 shown, two positioning grooves 15 are formed on the inner surface of the separation barrel 4, two positioning rods 16 are fixedly connected to the outer surface of the sleeve 14, and the outer surfaces of the two positioning rods 16 are respectively slidably connected to the inner surfaces of the positioning grooves 15. By setting the positioning grooves 15 and the positioning rods 16, the sleeve 14 can be limited, so that it can rotate together with the separation barrel 4.

[0024] As Figure 2 and Figure 3As shown, a stabilizing frame 12 is fixedly connected to the bottom end of the sealing cover 2, and the bottom end of the separation barrel 4 is rotatably connected to the inner bottom surface of the stabilizing frame 12. By providing the stabilizing frame 12, the separation barrel 4 can rotate more stably.

[0025] As Figure 2 and Figure 3 shown, two fixing seats 10 are fixedly connected to the inner surface of the separation tank 1, and the bottom ends of the two reciprocating lead screws 9 are respectively rotatably connected to the inside of the two fixing seats 10, which can position the reciprocating lead screws 9.

[0026] As Figure 2 and Figure 3 shown, a blanking pipe 13 is fixedly communicated with the bottom end of the separation tank 1, and a handle 21 is fixedly connected to the top end of the pressing cover 18. By providing the blanking pipe 13, the material can be discharged, and by providing the handle 21, it is convenient to press and seal the pressing cover 18.

[0027] As Figure 1 shown, a lifting handle 17 is fixedly connected to the top end of the sleeve 14, and the outer surface of the sleeve 14 is in interference fit with the inner surface of the separation barrel 4. By providing the lifting handle 17, it is convenient to take out the sleeve 14.

[0028] The present utility model provides a protein separation device, and the specific working principle is as follows:

[0029] Insert the sleeve 14 filled with the material into the separation barrel 4, then fix the pressing cover 18 to the mounting frame 5 so that the sealing plate 20 presses and seals the sleeve 14. Then drive the motor 6 to drive the driving gear 7 to rotate, so that the rotating gear 3 rotates accordingly and drives the separation barrel 4 to rotate, separating the macromolecular protein and the mixed liquid in the separation barrel 4. During the separation process, the rotating gear 3 drives the driven gear 8 to rotate, so that the reciprocating lead screw 9 rotates to drive the scraping ring 11 to slide up and down on the inner wall of the separation tank 1, quickly converging the small molecular protein and the accumulated liquid attached to the inner wall. The converged mixed liquid quickly falls to the bottom of the separation tank 1 under the action of gravity and flows out through the blanking pipe 13 for collection. When the separation is completed, rotate the pressing cover 18 to disengage it from the mounting frame 5 and take it out together with the sealing plate 20. Then, pull the lifting handle 17 to pull out the sleeve 14 from the inside of the separation barrel 4, and insert the next sleeve 14 filled with the raw material to be separated into the separation barrel 4. After fixing, the next separation can be carried out.

[0030] Although the embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A protein separation device, comprising a separation tank (1), characterized in that: The top of the separation tank (1) is fixedly connected to a sealing cover (2), the inside of the sealing cover (2) is rotatably connected to a rotating gear (3), the bottom end of the rotating gear (3) is fixedly connected to a separation barrel (4), the top of the sealing cover (2) is fixedly connected to a mounting frame (5), one side of the mounting frame (5) is fixedly connected to a driving motor (6), the output end of the driving motor (6) is transmission-connected to a driving gear (7), the outer surface of the driving gear (7) is meshed with the outer surface of the rotating gear (3), the top of the sealing cover (2) is rotatably connected to two driven gears (8), the outer surfaces of the two driven gears (8) are meshed with the outer surface of the rotating gear (3), the bottom ends of the two driven gears (8) pass through the top of the sealing cover (2) and are transmission-connected to a reciprocating screw rod (9), the outer surfaces of the two reciprocating screw rods (9) are meshed with scraper rings (11), and the outer surface of the scraper ring (11) is tightly fitted with the inner surface of the separation tank (1).

2. A protein separation device according to claim 1, characterized in that: The separation barrel (4) is internally sleeved with a sleeve (14), the mounting frame (5) is internally threadedly connected with a clamping cover (18), the bottom end of the clamping cover (18) is fixedly connected with a connecting column (19), the bottom end of the connecting column (19) is rotatably connected with a sealing plate (20), and the bottom end of the sealing plate (20) is in tight contact with the top end of the sleeve (14).

3. A protein separation device according to claim 2, characterized in that: The inner surface of the separation barrel (4) is provided with two positioning grooves (15), the outer surface of the sleeve (14) is fixedly connected with two positioning rods (16), and the outer surfaces of the two positioning rods (16) are respectively slidably connected with the inner surfaces of the positioning grooves (15).

4. A protein separation device according to claim 1, characterized in that: The bottom end of the sealing cover (2) is fixedly connected to a stabilizing frame (12), and the bottom end of the separation barrel (4) is rotatably connected to the inner bottom surface of the stabilizing frame (12).

5. A protein separation device according to claim 4, characterized in that: Two fixing seats (10) are fixedly connected to the inner surface of the separation tank (1), and the bottom ends of the two reciprocating screw rods (9) are rotatably connected to the inside of the two fixing seats (10) respectively.

6. A protein separation device according to claim 2, characterized in that: The bottom end of the separation tank (1) is fixedly connected to a feed pipe (13), and the top end of the compression cover (18) is fixedly connected to a handle (21).

7. A protein separation device according to claim 6, characterized in that: A handle (17) is fixedly connected to the top of the sleeve (14), and the outer surface of the sleeve (14) is interference fit with the inner surface of the separation barrel (4).

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