Magnetic bead method nucleic acid extraction magnetic sleeve applicable to on-site rapid detection

Abstract

The utility model relates to the technical field of in-vitro diagnosis, in particular to a magnetic bead method nucleic acid extraction magnetic sleeve suitable for on-site rapid detection. The utility model discloses a magnetic bead method nucleic acid extraction magnetic sleeve suitable for on-site rapid detection, which is characterized by comprising a hollow circular tube (1), a high-elasticity O-shaped ring (2), a magnet (3) and a tube bottom cover (4), the outer diameter of the high-elasticity O-shaped ring (2) and the outer diameter of the magnet (3) are approximately equal to the inner diameter of the hollow circular tube (1), and the outer diameter of the high-elasticity O-shaped ring (2) and the outer diameter of the magnet (3) are equal to the inner diameter of the hollow circular tube (1). The high-elasticity O-shaped ring (2) is arranged on the magnet (3) and is arranged in an inner cavity at the bottom end of the hollow circular tube (1), and the tube bottom cover (4) seals the bottom end of the hollow circular tube (1). The nucleic acid extraction device disclosed by the utility model is ingenious in design and simple and reasonable in structure, the operation of removing washing waste liquid by a pipettor is omitted, the loss of sample nucleic acid is reduced, and the nucleic acid extraction device is convenient to carry and is particularly suitable for nucleic acid extraction in field detection.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro diagnosis, and particularly relates to a magnetic sleeve for nucleic acid extraction by magnetic bead method suitable for on-site rapid detection. Background Art

[0002] The magnetic bead method for nucleic acid extraction has been widely used in the field of genetic engineering. The magnetic bead method for nucleic acid extraction lyses the sample with a lysis solution, and the nucleic acid molecules released from the sample are specifically adsorbed onto the surface of magnetic particles, while impurities such as proteins are not adsorbed and remain in the solution. The magnetic beads carrying nucleic acid are mixed with the liquid by repeated rapid stirring, and pure nucleic acid is finally obtained through steps such as lysis, nucleic acid adsorption, washing and elution.

[0003] In the case of a small sample volume, a magnetic stand is required for manual magnetic bead method to extract nucleic acid. The structures of the magnetic stands on the market are not the same, and they are heavy and large in volume. However, there are certain problems with the current magnetic stands. First, when using the existing magnetic stand, during the washing step, a common pipette is used to remove the washing waste liquid, and the magnetic beads are easily sucked in due to the suction of the pipette, reducing the nucleic acid extraction effect. Second, the existing magnetic stands are heavy and large in volume, not convenient to carry, and not suitable for the needs of on-site rapid detection. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a magnetic sleeve for nucleic acid extraction by magnetic bead method suitable for on-site rapid detection, which solves the problem of manual magnetic bead method for nucleic acid extraction in current on-site detection.

[0005] A magnetic sleeve for nucleic acid extraction by magnetic bead method suitable for on-site rapid detection includes a hollow round tube and a magnet. The outer diameter of the magnet is approximately equal to the inner diameter of the hollow round tube, and the magnet is placed in the inner cavity at the bottom end of the hollow round tube.

[0006] In a preferred embodiment, the magnetic sleeve for nucleic acid extraction by magnetic bead method includes a hollow round tube, a highly elastic O-ring, a magnet, and a bottom cover of the tube. The outer diameter of the highly elastic O-ring and the outer diameter of the magnet are approximately equal to the inner diameter of the hollow round tube. The highly elastic O-ring is placed in the inner cavity at the bottom end of the hollow round tube above the magnet, and the bottom cover of the tube closes the bottom end of the hollow round tube.

[0007] In a preferred embodiment, in order to prevent the O-ring from slipping off, a limiting structure is provided on the inner cavity wall of the hollow round tube, preferably a limiting convex ring.

[0008] In a preferred embodiment, the inner diameter of the highly elastic O-ring is 1 - 3 cm, which is suitable for the sample collection and extraction tube diameters commonly used in this field and can play a fixing role.

[0009] In a preferred embodiment, the magnet is a cylindrical permanent magnet or a rectangular parallelepiped permanent magnet, preferably a hollow cylindrical permanent magnet with a diameter of 1 - 3 cm and a thickness of 0.1 - 0.3 cm.

[0010] In a preferred embodiment, the bottom cover of the tube closes the bottom end of the hollow circular tube by means of a clamp or a threaded closing structure.

[0011] In a preferred embodiment, the bottom cover of the tube is a flat circular cover.

[0012] In a preferred embodiment, the diameter of the bottom cover of the tube is larger than the diameter of the hollow circular tube, preferably 1.5 - 3 cm in diameter, which can ensure the static stability of the magnetic bead nucleic acid extraction magnetic sleeve.

[0013] Compared with the prior art, the utility model is ingeniously designed, has a simple and reasonable structure, omits the operation of removing the washing waste liquid by a pipette, reduces the loss of sample nucleic acid and is convenient to carry, and is particularly suitable for on-site nucleic acid extraction and detection purposes. Description of the Drawings

[0014] Figure 1 Schematic longitudinal sectional view of a preferred example of the utility model. Detailed Description of the Invention

[0015] See Figure 1 As shown, a preferred embodiment of the utility model:

[0016] A magnetic bead nucleic acid extraction magnetic sleeve suitable for on-site rapid detection, comprising a hollow circular tube 1, a highly elastic O-ring 2, a magnet 3, and a bottom cover 4 of the tube. The outer diameter of the highly elastic O-ring 2 and the outer diameter of the magnet 3 are approximately equal to the inner diameter of the hollow circular tube 1. The highly elastic O-ring 2 is placed in the inner cavity at the bottom end of the hollow circular tube 1 above the magnet 3, and the bottom cover 4 of the tube closes the bottom end of the hollow circular tube 1. A limiting convex ring 101 is provided on the inner cavity wall of the hollow circular tube 1. The inner diameter of the highly elastic O-ring 2 is 0.3 - 0.8 cm. The magnet 3 is a hollow cylindrical permanent magnet with a diameter of 0.5 - 1 cm and a thickness of 0.1 - 0.3 cm. The bottom cover 4 of the tube is a circular cover with a diameter of 1.5 - 3 cm, and the bottom cover 4 of the tube closes the bottom end of the hollow circular tube 1 by means of a clamp closing structure.

[0017] The utility model is used for nucleic acid extraction, without the need for other instrument devices, and only requires the following 5 simple operations: (1) adding samples and lysing; (2) binding and removing impurities; (3) washing impurities; (4) drying; (5) eluting.

[0018] The specific operation process is as follows:

[0019] 1. Shake up and down to fully mix the sample collection and extraction tube containing the sample, so that the sample is fully lysed to release nucleic acid and bind to magnetic beads.

[0020] 2. Put the magnetic bead nucleic acid extraction magnetic sleeve of the present invention on the sample collection and extraction tube sleeve. After gently mixing up and down, let it stand for 3 to 5 minutes. When the liquid becomes clear, pour out all the solution.

[0021] 3. After dropping 1.5 - 2 mL of 75 - 80% alcohol into the sample sampling tube, put on the magnetic bead nucleic acid extraction magnetic sleeve of the present invention, seal the tube cap, gently mix up and down to allow the magnetic beads to adsorb to the tube wall. After shaking, it can be left standing with a magnet for 3 to 5 minutes, and then pour out all the solution and remove the magnetic bead nucleic acid extraction magnetic sleeve of the present invention.

[0022] 4. Open the lid of the sample collection and extraction tube and place it at 50 - 100 °C for 5 - 10 minutes to dry the residual alcohol, or it can be air-dried naturally.

[0023] 5. Add 8 - 10 drops of nucleic acid elution solution, cover the tube cap and mix by shaking up and down, then let it stand. The supernatant after standing is the purified nucleic acid template, which can be used for subsequent qPCR nucleic acid detection reactions.

[0024] The scope of the present utility model is not limited by the specific embodiments described. The embodiments are only used as individual examples to illustrate various aspects of the present utility model. The scope of the present utility model also includes methods and components with equivalent functions. In fact, in addition to the content described herein, those skilled in the art can easily master various improvements to the present invention with reference to the above description and drawings. Such improvements also fall within the scope of the appended claims.

Claims

1. A magnetic cannula for nucleic acid extraction by magnetic bead method suitable for on-site rapid detection, characterized in that: It comprises a hollow circular tube (1) and a magnet (3), wherein the outer diameter of the magnet (3) is approximately equal to the inner diameter of the hollow circular tube (1), and the magnet (3) is placed in the inner cavity at the bottom end of the hollow circular tube (1).

2. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 1, characterized in that: The invention comprises a hollow circular tube (1), a highly elastic O-ring (2), a magnet (3), and a tube bottom cover (4); the outer diameter of the highly elastic O-ring (2) and the outer diameter of the magnet (3) are approximately equal to the inner diameter of the hollow circular tube (1); the highly elastic O-ring (2) is placed above the magnet (3) in the inner cavity of the bottom end of the hollow circular tube (1); and the tube bottom cover (4) seals the bottom end of the hollow circular tube (1).

3. The magnetic sleeve for nucleic acid extraction by magnetic bead method as claimed in claim 1, characterized in that: The inner cavity wall of the hollow circular tube is provided with a limiting structure.

4. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 3, characterized in that: The limiting structure is a limiting convex ring (101).

5. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 2, characterized in that: The high elasticity O-ring (2) has an inner diameter of 1 to 3 cm.

6. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 1, characterized in that: The magnet (3) is a cylindrical permanent magnet or a rectangular permanent magnet.

7. The magnetic sleeve for nucleic acid extraction by magnetic bead method as claimed in claim 6, characterized in that: The magnet (3) is a hollow cylindrical permanent magnet with a diameter of 1 to 3 cm and a thickness of 0.1 to 0.3 cm.

8. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 2, characterized in that: The bottom cover (4) of the tube closes the bottom end of the hollow round tube (1) by adopting a clamp or a threaded closing structure.

9. The magnetic sleeve for extracting nucleic acid by magnetic beads as claimed in claim 8, characterized in that: The tube bottom cover (4) is a round cover.

10. The magnetic sleeve for nucleic acid extraction by magnetic bead method according to claim 9, characterized in that: The diameter of the tube bottom cover (4) is greater than the diameter of the hollow circular tube (1).

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