Dental pulp stem cell separation and extraction device

Abstract

The utility model discloses a dental pulp stem cell separation and extraction device which comprises a bottom table, the top of the bottom table is fixedly connected with a protective shell, the top of the protective shell is in threaded connection with a top cover, the interior of the bottom table is fixedly connected with a motor, the interior of the protective shell is provided with a rotary table, and an output shaft of the motor penetrates through the top of the bottom table to be fixedly connected with the rotary table. A plurality of circular grooves are formed in the top of the rotary table, soft rubber cylinders are fixedly connected into the circular grooves, a sliding piece is fixedly connected to the outer side of the rotary table, an annular guide rail is slidably connected to the outer side of the sliding piece, and the annular guide rail is fixedly connected with the inner wall of the protective shell. The motor is started to enable the rotary table to rotate, the test tube is driven to rotate, the centrifugal separation purpose is achieved, the annular guide rail and the sliding piece are used in cooperation, the rotary stability of the rotary table and the test tube can be guaranteed, the rotary table is prevented from shaking during rotation, and the centrifugal separation effect is guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of centrifugal separation and extraction, and specifically relates to a device for separating and extracting dental pulp stem cells. Background Art

[0002] Stem cells are a class of cells with unlimited or immortal self-renewal ability, and can produce at least one type of highly differentiated progeny cells. Over the years, the definition of stem cells has been continuously revised and defined from different aspects. Most biologists and medical scientists believe that stem cells are a class of cells derived from embryos, fetuses or adults that have the ability to self-renew and proliferate and differentiate without limit under certain conditions. They can produce daughter cells with the same phenotype and genotype as themselves, and can also produce specialized cells that make up the tissues and organs of the body. At the same time, they can also differentiate into progenitor cells.

[0003] Dental pulp stem cells are one of many stem cells. When separating and extracting them, centrifugal separation and extraction are usually carried out by rotation. In the prior art, Chinese Patent CN217973194U discloses a device for separating and extracting stem cells, specifically related to the technical field of stem cells, including a separation tank and an upper cover located above the separation tank. A motor is fixedly installed at the bottom of the separation tank, and the output end of the motor is fixedly connected to a connection disk through a rotating shaft. A fixed sleeve is fixedly connected to the center of the top of the connection disk, and a plurality of positioning sleeves are sequentially sleeved on the outer surface of the fixed sleeve. A bearing disk is fixedly connected to the outer surface of each positioning sleeve. This design enables the stem cell separation and extraction device to realize the overall removal of the bearing disk through the mutual cooperation of the positioning sleeve, the fixed sleeve, the bearing disk and the positioning plate. Positioning rings with different diameters and the clamping mechanisms on the positioning rings can be produced according to actual situations, which is convenient for better adapting to test tubes of different sizes. At the same time, the bearing disk can be taken out as a whole, which is more convenient for users to place or take out test tubes, and there is no need to always operate facing the centrifuge.

[0004] However, when the stem cell separation and extraction device provided in the above technical solution is actually used, there are still many disadvantages. For example, when rotating the test tube, there is no limit structure on the outside, resulting in poor rotation stability, making the test tube easy to shake during rotation, which affects the centrifugal separation effect. Summary of the Utility Model

[0005] The purpose of this part is to outline some aspects of the embodiments of the present utility model and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this part, as well as in the abstract and title of the specification of this application, to avoid obscuring the purpose of this part, the abstract of the specification and the title of the utility model. However, such simplifications or omissions cannot be used to limit the scope of the present utility model.

[0006] To solve the problem that when the separation and extraction device for stem cells rotates the test tube, there is no limit structure on the outside, resulting in poor rotation stability, easy shaking of the test tube during rotation, and affecting the centrifugal separation effect proposed in the above background technology, the present utility model adopts the following technical solutions.

[0007] A dental pulp stem cell separation and extraction device includes a base table. The top of the base table is fixedly connected with a protective shell, and the top of the protective shell is threadedly connected with a top cover. A motor is fixedly connected inside the base table. A turntable is arranged inside the protective shell, and the output shaft of the motor penetrates the top of the base table and is fixedly connected with the turntable. A plurality of circular grooves are arranged on the top of the turntable, and soft rubber cylinders are fixedly connected inside the plurality of circular grooves. A sliding piece is fixedly connected to the outside of the turntable, and the outside of the sliding piece is slidably connected with an annular guide rail, and the annular guide rail is fixedly connected with the inner wall of the protective shell.

[0008] Preferably, a ring groove is opened on the top of the base table, and a sliding column is slidably connected to the top of the ring groove. The sliding column is fixedly connected to the bottom of the turntable.

[0009] Preferably, the central axis of the turntable coincides with the central axis of the base table. The sliding column and the sliding piece are both annularly and evenly distributed about the central axis of the turntable. The base table, the protective shell, and the top cover are coaxially arranged.

[0010] Preferably, a movable cover is rotatably connected to the center of the top cover. A plurality of threaded rods are threadedly connected to the movable cover. The bottom ends of the threaded rods are fixedly connected with pressing pieces. The positions of the pressing pieces correspond to the positions of the soft rubber cylinders. The central axis of the movable cover coincides with the central axis of the top cover. The plurality of pressing pieces are annularly and evenly distributed about the central axis of the movable cover. The plurality of soft rubber cylinders are annularly and evenly distributed about the central axis of the turntable.

[0011] Preferably, fixing blocks are arranged on both sides of the soft rubber cylinder. The fixing blocks are fixedly connected with the turntable. Telescopic rods are fixedly connected to both sides of the fixing blocks. The ends of the telescopic rods are fixedly connected with clamping blocks. Springs are sleeved on the outside of the telescopic rods. The two ends of the springs are respectively fixedly connected with the clamping blocks and the fixing blocks.

[0012] Preferably, a plurality of heat dissipation openings are opened on the outside of the base table. The plurality of heat dissipation openings are annularly and evenly distributed about the central axis of the base table.

[0013] Preferably, a plurality of reinforcing bars are arranged on the outside of the protective shell. The plurality of reinforcing bars and the protective shell form an integral structure. The plurality of reinforcing bars are annularly and evenly distributed about the central axis of the protective shell.

[0014] Beneficial effects

[0015] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0016] The utility model rotates the turntable by starting the motor, drives the test tube to rotate, and achieves the purpose of centrifugal separation. The combined use of the annular guide rail and the sliding piece can ensure the rotation stability of the turntable and the test tube, prevent the turntable from shaking during rotation, and ensure the centrifugal separation effect. The combined use of the annular groove and the sliding column can improve the rotation stability of the bottom of the turntable. Rotating the threaded rod on the rotary movable cover causes the pressing piece to descend, so that the pressing piece presses on the top of the test tube, which can ensure the stability of the test tube during rotation and further improve the stability of the device during centrifugation. Description of the Drawings

[0017] Figure 1 It is a schematic three-dimensional structure diagram of the whole of the utility model;

[0018] Figure 2 It is a schematic three-dimensional structure diagram of the turntable of the utility model;

[0019] Figure 3 It is a schematic front view structure diagram of the turntable of the utility model;

[0020] Figure 4 It is a schematic three-dimensional structure diagram of the bottom platform of the utility model;

[0021] Figure 5 It is a schematic partial three-dimensional structure diagram of the utility model;

[0022] Figure 6 It is a schematic three-dimensional structure diagram of the movable cover of the utility model;

[0023] Figure 7 For the utility model Figure 3 The enlarged structure diagram at A in it.

[0024] The corresponding relationship between the reference numerals and the component names in the drawings is as follows:

[0025] 1. Bottom platform; 2. Protective shell; 3. Top cover; 4. Motor; 5. Turntable; 6. Soft rubber cylinder; 7. Sliding piece; 8. Annular guide rail; 9. Annular groove; 10. Sliding column; 11. Movable cover; 12. Threaded rod; 13. Pressing piece; 14. Fixed block; 15. Expansion rod; 16. Clamping block; 17. Heat dissipation port; 18. Reinforcing strip; 19. Spring. Detailed Embodiment

[0026] To make the above objects, features, and advantages of the utility model more obvious and understandable, the following detailed description of the specific embodiments of the utility model is given in conjunction with the drawings in the specification.

[0027] In the following description, many specific details are set forth in order to provide a thorough understanding of the present utility model. However, the present utility model may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the connotation of the present utility model. Therefore, the present utility model is not limited by the specific embodiments disclosed below.

[0028] Secondly, the so-called "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation manner of the present utility model. The appearances of "in one embodiment" in different places in this specification do not all refer to the same embodiment, nor are they separate or alternative embodiments that are mutually exclusive of other embodiments. The present utility model provides the following embodiments.

[0029] Please refer to Figures 1-7 , an embodiment provided by the present utility model: a dental pulp stem cell separation and extraction device, including a base 1, a protective shell 2 is fixedly connected to the top of the base 1, a top cover 3 is threadedly connected to the top of the protective shell 2. The device can be placed on an external horizontal surface through the feet at the bottom of the base 1. Rotate the top cover 3 to remove it and open the protective shell 2. A motor 4 is fixedly connected to the inside of the base 1, a turntable 5 is arranged inside the protective shell 2, and the output shaft of the motor 4 penetrates through the top of the base 1 and is fixedly connected to the turntable 5. Multiple circular grooves are arranged on the top of the turntable 5, and soft rubber cylinders 6 are fixedly connected to the inside of the multiple circular grooves. Fixing blocks 14 are arranged on both sides of the soft rubber cylinder 6, and the fixing blocks 14 are fixedly connected to the turntable 5. Telescopic rods 15 are fixedly connected to both sides of the fixing blocks 14, and clamping blocks 16 are fixedly connected to the ends of the telescopic rods 15. A spring 19 is sleeved on the outside of the telescopic rod 15, and both ends of the spring 19 are fixedly connected to the clamping block 16 and the fixing block 14 respectively. A test tube containing dental pulp stem cell blanks is loaded into the soft rubber cylinder 6. By using the spring 19 and the telescopic rod 15, and in cooperation with the use of the clamping block 16, the top of the test tube can be clamped. The soft rubber cylinder 6 has a soft texture and can protect the test tube.

[0030] In this embodiment, a sliding piece 7 is fixedly connected to the outside of the turntable 5, and an annular guide rail 8 is slidably connected to the outside of the sliding piece 7. The annular guide rail 8 is fixedly connected to the inner wall of the protective shell 2. An annular groove 9 is opened on the top of the base 1, and a sliding column 10 is slidably connected to the top of the annular groove 9. The sliding column 10 is fixedly connected to the bottom of the turntable 5. The central axis of the turntable 5 coincides with the central axis of the base 1. The sliding column 10 and the sliding piece 7 are both annularly and evenly distributed about the central axis of the turntable 5. The base 1, the protective shell 2, and the top cover 3 are coaxially arranged. Start the motor 4 to make the turntable 5 rotate, drive the test tube to rotate, and achieve the purpose of centrifugal separation. The cooperation of the annular guide rail 8 and the sliding piece 7 can ensure the rotation stability of the turntable 5 and the test tube, prevent the turntable 5 from shaking during rotation, and ensure the centrifugal separation effect. The cooperation of the annular groove 9 and the sliding column 10 can improve the rotation stability of the bottom of the turntable 5.

[0031] In this embodiment, a movable cover 11 is rotatably connected to the center of the top cover 3. A plurality of threaded rods 12 are threadedly connected to the movable cover 11. A pressing piece 13 is fixedly connected to the bottom end of the threaded rod 12. The position of the pressing piece 13 corresponds to the position of the soft rubber cylinder 6. The central axis of the movable cover 11 coincides with the central axis of the top cover 3. The plurality of pressing pieces 13 are evenly distributed in a ring about the central axis of the movable cover 11. The plurality of soft rubber cylinders 6 are evenly distributed in a ring about the central axis of the turntable 5. Rotate the threaded rod 12 on the movable cover 11 to lower the pressing piece 13 so that the pressing piece 13 presses on the top of the test tube, which can ensure the stability of the test tube during rotation and further improve the stability of the device during centrifugation.

[0032] In this embodiment, a plurality of heat dissipation openings 17 are formed on the outer side of the bottom base 1. The plurality of heat dissipation openings 17 are evenly distributed in a ring about the central axis of the bottom base 1. A plurality of reinforcing strips 18 are arranged on the outer side of the protective shell 2. An integrated structure is formed between the plurality of reinforcing strips 18 and the protective shell 2. The plurality of reinforcing strips 18 are evenly distributed in a ring about the central axis of the protective shell 2. The arrangement of the heat dissipation openings 17 can facilitate the heat dissipation of the motor 4 inside the bottom base 1. The use of the reinforcing strips 18 can improve the compressive resistance of the protective shell 2 and enhance the protection ability of the protective shell 2 for the components inside it.

[0033] Working principle: When using this device, first, the device can be placed on an external horizontal surface through the feet at the bottom of the bottom base 1. Rotate the top cover 3 to remove it, open the protective shell 2, and load the test tube containing the dental pulp stem cell blank into the soft rubber cylinder 6. By using the spring 19 and the telescopic rod 15, and cooperating with the use of the clamping block 16, the top of the test tube can be clamped. The soft rubber cylinder 6 has a soft texture and can protect the test tube. Start the motor 4 to make the turntable 5 rotate, driving the test tube to rotate to achieve the purpose of centrifugal separation. The cooperation of the annular guide rail 8 and the sliding piece 7 can ensure the rotational stability of the turntable 5 and the test tube, prevent the turntable 5 from shaking during rotation, and ensure the effect of centrifugal separation. The cooperation of the annular groove 9 and the sliding column 10 can improve the rotational stability of the bottom of the turntable 5. Rotate the threaded rod 12 on the movable cover 11 to lower the pressing piece 13 so that the pressing piece 13 presses on the top of the test tube, which can ensure the stability of the test tube during rotation and further improve the stability of the device during centrifugation. The arrangement of the heat dissipation openings 17 can facilitate the heat dissipation of the motor 4 inside the bottom base 1. The use of the reinforcing strips 18 can improve the compressive resistance of the protective shell 2 and enhance the protection ability of the protective shell 2 for the components inside it.

[0034] The above content further elaborates on the present utility model in combination with specific embodiments. It cannot be determined that the specific implementation of the present utility model is only limited to these descriptions. For those of ordinary skill in the technical field to which the present utility model pertains, without departing from the concept of the present utility model, several simple deductions or substitutions can still be made, which should all be regarded as falling within the protection scope determined by the claims submitted for the present utility model.

Claims

1. A dental pulp stem cell separation and extraction device, comprising a base (1), a protective shell (2) fixedly connected to the top of the base (1), a top cover (3) threadedly connected to the top of the protective shell (2), characterized in that: The interior of the base (1) is fixedly connected to a motor (4), the interior of the protective shell (2) is provided with a turntable (5), the output shaft of the motor (4) passes through the top of the base (1) and is fixedly connected to the turntable (5), the top of the turntable (5) is provided with a plurality of circular grooves, the interiors of the plurality of circular grooves are all fixedly connected to a soft rubber tube (6), the outer side of the turntable (5) is fixedly connected to a slide plate (7), the outer side of the slide plate (7) is slidably connected to an annular guide rail (8), and the annular guide rail (8) is fixedly connected to the inner wall of the protective shell (2).

2. The dental pulp stem cell separation and extraction device according to claim 1, characterized in that: The top of the base (1) is provided with an annular groove (9), the top of the annular groove (9) is slidably connected with a sliding column (10), and the sliding column (10) is fixedly connected to the bottom of the turntable (5).

3. The dental pulp stem cell separation and extraction device according to claim 2, characterized in that: The central axis of the turntable (5) coincides with the central axis of the base (1); the sliding column (10) and the sliding plate (7) are evenly distributed in a ring shape about the central axis of the turntable (5); and the base (1), the protective shell (2) and the top cover (3) are coaxially arranged.

4. The dental pulp stem cell separation and extraction device according to claim 3, characterized in that: The center of the top cover (3) is rotatably connected to a movable cover (11), a plurality of threaded rods (12) are threadedly connected to the movable cover (11), a pressing sheet (13) is fixedly connected to the bottom end of the threaded rod (12), the position of the pressing sheet (13) corresponds to the position of the soft rubber tube (6), the central axis of the movable cover (11) coincides with the central axis of the top cover (3), the plurality of pressing sheets (13) are evenly distributed in a ring shape about the central axis of the movable cover (11), and the plurality of soft rubber tubes (6) are evenly distributed in a ring shape about the central axis of the turntable (5).

5. The dental pulp stem cell separation and extraction device according to any one of claims 1 to 4, characterized in that: Both sides of the soft rubber tube (6) are provided with fixed blocks (14), the fixed blocks (14) are fixedly connected to the turntable (5), both sides of the fixed blocks (14) are fixedly connected with telescopic rods (15), the ends of the telescopic rods (15) are fixedly connected with clamping blocks (16), the outer side of the telescopic rod (15) is sleeved with a spring (19), and the two ends of the spring (19) are fixedly connected to the clamping blocks (16) and the fixed blocks (14) respectively.

6. The dental pulp stem cell separation and extraction device according to claim 5, characterized in that: The outer side of the base (1) is provided with a plurality of heat dissipation openings (17), and the plurality of heat dissipation openings (17) are evenly distributed in a ring shape about the central axis of the base (1).

7. The dental pulp stem cell separation and extraction device according to claim 6, characterized in that: A plurality of reinforcing strips (18) are arranged on the outer side of the protective shell (2); the plurality of reinforcing strips (18) and the protective shell (2) form an integrated structure; the plurality of reinforcing strips (18) are evenly distributed in a ring shape about the central axis of the protective shell (2).

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