Stem cell separation and detection device

Abstract

The utility model discloses a stem cell separation and detection device, which relates to the technical field of stem cell separation and detection devices and comprises a base plate, a centrifugal machine, a flow cytometer, a mounting rack and a mounting rod, the centrifugal machine and the flow cytometer are both fixedly mounted at the upper end of the base plate, and a centrifugal cylinder is arranged on the inner side of the upper end of the centrifugal machine. A supporting rod is fixedly mounted at the upper end of the centrifugal machine, a hook is arranged at the upper end of the supporting rod, and the mounting rod is arranged on the front face of the supporting rod and arranged on the back face of the supporting rod. By the adoption of the structure, after the installation rod and the installation frame are connected at the position of the supporting rod, the clamping block is pushed to slide through rotation of the first hand-screwing bolt and threaded telescopic rotation of the first hand-screwing bolt, and due to the fact that the opposite faces of the clamping block and the installation rod are provided with the V-shaped grooves, the clamping block can be rapidly installed and fixed on the supporting rod conveniently; and the operation can be carried out without a tool, so that the operation is simple and convenient.

Description

Technical Field

[0001] This utility model belongs to the technical field of stem cell separation and detection devices, and specifically relates to a stem cell separation and detection device. Background Technology

[0002] Umbilical cord blood, the blood from the umbilical cord and the blood vessels near the fetus in the placenta at birth, contains a large number of stem cells. The lymphocytes in cord blood have weak antigenicity and relatively immature function, resulting in a lower risk of acute graft-versus-host disease after transplantation. It has become an important route for stem cell transplantation and has wide applications in the treatment of hematologic malignancies, aplastic anemia, and some genetic diseases. Before cord blood stem cells are used, they need to be separated, tested, and comprehensively evaluated to improve safety. Cord blood needs to be cryopreserved after collection, so it must be thawed in a 37°C water bath before separation. After thawing, it is transferred to a centrifuge for separation. The process of thawing and transferring to the centrifuge is prone to contamination or leakage. Furthermore, the thawing process takes time, resulting in slow overall separation and testing efficiency.

[0003] A search revealed that Chinese patent CN220643134U discloses an umbilical cord blood stem cell separation and detection device, comprising: a main body component; an acceleration component; the acceleration component includes a clamp, a fastening bolt for locking the clamp, a movable groove on the front side of the clamp, two sets of sliders movably connected in the movable groove, a spring connected between the sliders, a constant temperature heating plate connected to the front end of the slider, a control line connected to the rear side of the constant temperature heating plate, a heat spreader connected to the inner side of the constant temperature heating plate, a tube groove on the lower end of the constant temperature heating plate, and telescopic rods connected to the lower ends of the two sets of constant temperature heating plates, with two sets of telescopic rods respectively connected to the two corners of the lower end of the constant temperature heating plate.

[0004] Compared to traditional methods, the aforementioned existing technical solutions no longer involve the issue of transport and dissolution, representing a significant improvement in usability. However, some shortcomings still exist in practical applications. For instance, the use of a slider connected to a spring allows two constant-temperature heating plates on the slider to clamp the blood bag and provide constant-temperature heating, gradually dissolving the umbilical cord blood inside for easier centrifuge separation and thus improving testing efficiency. However, the spring force is difficult to control; excessive force can damage the blood bag, while insufficient force can affect the contact between the constant-temperature heating plate and the blood bag, making it somewhat cumbersome to use. Secondly, the use of multiple fastening bolts and clamps on the support rod for installation, coupled with post-installation adjustments, requires users to expend considerable effort before and after operation to loosen and tighten the bolts, thus making installation and use still not convenient enough. Utility Model Content

[0005] In view of the problems mentioned in the background art, the purpose of this utility model is to provide a stem cell separation and detection device to solve the problems mentioned in the background art.

[0006] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0007] A stem cell separation and detection device includes a substrate, a centrifuge, a flow cytometer, a mounting frame, and mounting rods. The centrifuge and flow cytometer are both fixedly mounted on the upper end of the substrate. A centrifuge tube is located on the inner side of the upper end of the centrifuge. A support rod is fixedly mounted on the upper end of the centrifuge, and a hook is provided at the upper end of the support rod. The mounting rod is located on the front side of the support rod, and the mounting frame is located on the back side of the support rod. Connecting blocks are symmetrically fixedly mounted on the back side of the mounting rod. Connecting grooves are symmetrically formed on the front side of the mounting frame. The mounting rod is inserted into the connecting blocks and connecting grooves. The inner side of the connecting blocks... The mounting bracket has a locking hole, and the mounting bracket is engaged with the locking hole through a connecting mechanism. A first hand-tightening bolt is threaded to the inner side of the back of the mounting bracket. The threaded end of the first hand-tightening bolt passes through the wall of the mounting bracket and is rotatably connected to a clamping block. The clamping block is slidably connected to the inner recess of the mounting bracket. V-shaped grooves are opened on the opposite surfaces of the clamping block and the mounting rod. A clamping mechanism is provided on the front of the mounting rod. A second hand-tightening bolt is provided on the outer side of the clamping mechanism. A constant temperature heating plate is fixedly installed on the clamping mechanism through the second hand-tightening bolt. An indicator light is provided on the outer side of the constant temperature heating plate.

[0008] The clamping mechanism includes a mounting groove, which is formed on the front of the mounting rod. A bidirectional screw is rotatably connected to the inner side of the mounting groove. A rotating block is fixedly installed at one end of the bidirectional screw through the inner wall of the mounting groove. A moving block is threadedly connected to the outer side of the bidirectional screw. One end of the moving block is slidably connected to the inner side of the mounting groove. The moving block is fixedly installed by a second hand-tightened bolt and a constant temperature heating plate.

[0009] The connecting mechanism includes a mounting cavity symmetrically located inside the mounting frame. A movable plate is slidably connected to the inner side of the mounting cavity. A locking block is fixedly installed on one side of the movable plate. The locking end of the locking block extends into the inner side of the connecting groove and engages with the locking hole of the connecting block. A pull rod is fixedly installed on the side of the movable plate away from the locking block. A pull ring is fixedly installed at the end of the pull rod away from the movable plate, which slides through the inner wall of the mounting cavity. A spring is sleeved on the outer side of the pull rod.

[0010] As a preferred technical solution, a second sliding groove is symmetrically provided on the inner side of the mounting groove, and one end of the moving block and the second sliding groove are slidably connected.

[0011] As a preferred technical solution, a mating groove is provided on the side of the movable block away from the mounting groove, and a mating block is fixedly installed on the outer side of the constant temperature heating plate. The mating block and the mating groove are connected by an insertion. The movable block is fixedly installed with the mating block by a second hand-tightened bolt.

[0012] As a preferred technical solution, the mounting bracket has a screw hole on its inner side, one end of the first hand-tightening bolt is threaded through the screw hole, the mounting bracket has a first sliding groove symmetrically formed in its inner recess, and the end of the clamping block is slidably connected to the inner side of the first sliding groove.

[0013] As a preferred technical solution, a third sliding groove is symmetrically provided on the inner side of the mounting cavity, and the movable plate and the third sliding groove are slidably connected.

[0014] As a preferred technical solution, a sliding hole is provided on one side of the inner wall of the mounting cavity, and one end of the pull rod slides through the sliding hole.

[0015] In summary, the present invention has the following main advantages:

[0016] First, after connecting the mounting rod and mounting bracket to the support rod, the first hand-tightening bolt is rotated. The thread of the first hand-tightening bolt extends and rotates, pushing the clamping block to slide. Since the clamping block and the mounting rod have V-shaped grooves on their opposite surfaces, it is easy to quickly install and fix them on the support rod. When adjusting after installation, you only need to loosen or tighten the first hand-tightening bolt. No tools are required, making it simple and convenient.

[0017] Second, place the mounting rod on one side of the support rod, and then insert the mounting bracket from the other side of the mounting rod. When the connecting block on the mounting rod is inserted into the connecting groove, one end of the connecting block presses against the inclined surface of the locking block, causing the movable plate connected by the locking block to slide and compress the spring in the mounting cavity. When the locking end of the locking block aligns with the locking hole of the connecting block, the spring returns to its original position and pushes the movable plate to move, allowing the locking block on the movable plate to be inserted into the locking hole for fixation. This allows for quick and easy connection of the mounting rod and the mounting bracket.

[0018] Third, the double-ended screw is rotated in the mounting groove of the mounting rod by rotating the handle. The rotation of the double-ended screw causes the two moving blocks connected by the outer thread to slide closer or further apart. When they move away from each other, the blood bag is placed between the two constant temperature heating plates connected by the moving blocks. When they move closer, they can be adjusted and clamped according to the condition of the blood bag, so that the blood bag is not easily damaged and there is no need for subsequent maintenance, which greatly enhances the practicality of the device. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model;

[0020] Figure 2 This is the utility model Figure 1Enlarged view of point A in the middle;

[0021] Figure 3 This is a partial exploded view of the present invention;

[0022] Figure 4 This is a schematic diagram of the connection mechanism of this utility model.

[0023] Reference numerals: 1. Base plate; 2. Flow cytometer; 3. Centrifuge; 4. Centrifuge tube; 7. Support rod; 701. Hook; 9. Mounting frame; 10. Mounting rod; 11. Connecting mechanism; 111. Mounting cavity; 112. Pull rod; 113. Pull ring; 114. Spring; 115. Movable plate; 116. Locking block; 12. First hand-tightening bolt; 13. Clamping mechanism; 131. Rotating block; 132. Mounting groove; 133. Moving block; 134. Bidirectional screw; 14. Constant temperature heating plate; 15. Indicator light; 16. Clamping block; 17. V-groove; 18. Connecting groove; 19. Connecting block; 20. Locking hole; 21. Screw hole; 22. First sliding groove; 23. Second hand-tightening bolt; 24. Second sliding groove; 25. Mating block; 26. Mating groove; 27. Third sliding groove; 28. Sliding hole. Detailed Implementation

[0024] refer to Figures 1 to 4The stem cell separation and detection device described in this embodiment includes a substrate 1, a centrifuge 3, a flow cytometer 2, a mounting frame 9, and a mounting rod 10. The centrifuge 3 and the flow cytometer 2 are both fixedly mounted on the upper end of the substrate 1. A centrifuge cylinder 4 is provided on the inner side of the upper end of the centrifuge 3. A support rod 7 is fixedly mounted on the upper end of the centrifuge 3, and a hook 701 is provided on the upper end of the support rod 7. The mounting rod 10 is located on the front side of the support rod 7, and the mounting frame 9 is located on the back side of the support rod 7. Connecting blocks 19 are symmetrically fixedly mounted on the back side of the mounting rod 10, and connecting grooves 1 are symmetrically opened on the front side of the mounting frame 9. 8. The mounting rod 10 is inserted into the connecting block 19 and the connecting groove 18. The inner side of the connecting block 19 has a locking hole 20. The mounting frame 9 is locked into the locking hole 20 through the connecting mechanism 11. The inner side of the back of the mounting frame 9 is threaded with a first hand-tightening bolt 12. The threaded end of the first hand-tightening bolt 12 passes through the frame wall of the mounting frame 9 and is rotatably connected to a clamping block 16. The clamping block 16 is slidably connected to the inner recess of the mounting frame 9. V-grooves 17 are opened on the opposite surfaces of the clamping block 16 and the mounting rod 10. The front of the mounting rod 10 is provided with a clamping mechanism 13. A second hand-tightening bolt 23 is provided on the outer side, and a constant temperature heating plate 14 is fixedly installed on the clamping mechanism 13 by the second hand-tightening bolt 23. An indicator light 15 is provided on the outer side of the constant temperature heating plate 14. The centrifuge cylinder 4 of the centrifuge 3 is also provided with a liquid inlet, which facilitates the simultaneous input of dissolved umbilical cord blood into the centrifuge 3 before centrifugation through the tubing on the blood bag. For specific operation and usage methods, please refer to the umbilical cord blood stem cell separation and detection device disclosed in Chinese Patent No. CN220643134U. The flow cytometer 2 is used to perform the separation of the detection solution after the separation. Cell detection is a known existing technology. After connecting the mounting rod 10 and the mounting bracket 9 at the support rod 7, the first hand-tightening bolt 12 is rotated. The thread of the first hand-tightening bolt 12 extends and retracts, pushing the clamping block 16 to slide. Since the clamping block 16 and the mounting rod 10 have V-grooves 17 on their opposite sides, it is convenient to quickly install and fix them on the support rod 7. When adjusting after installation, it is only necessary to loosen or tighten the first hand-tightening bolt 12. No tools are required for operation, which is simple and convenient. The V-groove 17 also has a rubber layer, which can increase friction and improve the stability of the fixation.

[0025] The clamping mechanism 13 includes a mounting groove 132, which is formed on the front of the mounting rod 10. A bidirectional screw 134 is rotatably connected to the inner side of the mounting groove 132. One end of the bidirectional screw 134 passes through the inner wall of the mounting groove 132 and is fixedly mounted with a rotating block 131. A moving block 133 is threadedly connected to the outer side of the bidirectional screw 134. One end of the moving block 133 is slidably connected to the inner side of the mounting groove 132. The moving block 133 is fixedly mounted by a second hand-tightening bolt 23 and a constant temperature heating plate 14. When clamping the blood bag, it is only necessary to rotate the bidirectional screw 134 in the mounting groove 132 of the mounting rod 10 using the handle. The rotation of the bidirectional screw 134 causes the two moving blocks 133 connected by the outer threads to move closer to each other or to each other. After the blood bag slides away from each other, it is placed between the two constant temperature heating plates 14 connected by the moving block 133. After they move closer together, they can be adjusted and clamped according to the condition of the blood bag, so as not to damage the blood bag and not to involve subsequent maintenance issues, which greatly enhances the practicality of the device. The thread structure at both ends of the bidirectional screw 134 is opposite. The indicator light 15 on the constant temperature heating plate 14 is connected to the circuit. When it is activated, the indicator light 15 lights up to indicate that the constant temperature heating plate 14 is in the heating state. If the indicator light 15 does not light up, it means that the constant temperature heating plate 14 is faulty and cannot heat. When replacing it, you only need to loosen the second hand-tightening bolt 23 to remove the constant temperature heating plate 14 from the moving block 133, which is convenient for maintenance.

[0026] The connecting mechanism 11 includes a mounting cavity 111, which is symmetrically located inside the mounting frame 9. A movable plate 115 is slidably connected to the inner side of the mounting cavity 111. A locking block 116 is fixedly installed on one side of the movable plate 115. The locking end of the locking block 116 extends into the inner side of the connecting groove 18 and engages with the locking hole 20 of the connecting block 19. A pull rod 112 is fixedly installed on the side of the movable plate 115 away from the locking block 116. A pull ring 113 is fixedly installed on the end of the pull rod 112 away from the movable plate 115, which slides through the inner wall of the mounting cavity 111. A spring 114 is sleeved on the outer side of the pull rod 112. When installing and connecting the mounting rod 10 and the mounting frame 9 on the support rod 7, the mounting rod 10 is placed on one side of the support rod 7, and then the mounting frame 9 is inserted from the other side of the mounting rod 10. When the connecting block 19 on the mounting rod 10 is inserted into the connecting groove... At 18 o'clock, one end of the connecting block 19 presses against the inclined surface of the locking block 116, causing the movable plate 115 connected to the locking block 116 to slide and compress the spring 114 in the mounting cavity 111. When the locking end of the locking block 116 corresponds to the locking hole 20 of the connecting block 19, the spring 114 resets and pushes the movable plate 115 to move, so that the locking block 116 on the movable plate 115 can be inserted into the locking hole 20 for fixation, thereby facilitating the quick connection of the mounting rod 10 and the mounting frame 9. During disassembly, loosen the first hand-tightening bolt 12, lower the mounting rod 10 and the mounting frame 9, and then simultaneously pull the pull rings 113 on both sides of the mounting frame 9, so that the pull rings 113 drive the locking block 116 connected to the movable plate 115 to separate from the locking hole 20 on the connecting block 19, thereby pulling the mounting frame out of the mounting rod 10 to achieve disassembly.

[0027] refer to Figure 3 The inner side of the mounting groove 132 is symmetrically provided with a second sliding groove 24. One end of the moving block 133 is slidably connected to the second sliding groove 24. The moving block 133 can slide on the mounting groove 132 through the second sliding groove 24.

[0028] refer to Figure 3 The movable block 133 has a mating groove 26 on the side away from the mounting groove 132. The mating block 25 is fixedly installed on the outer side of the constant temperature heating plate 14. The mating block 25 and the mating groove 26 are connected by plugging. The movable block 133 is fixedly installed with the mating block 25 by the second hand-tightening bolt 23. By plugging the mating groove 26 on the movable block 133 and the mating block 25 on the constant temperature heating plate 14, the constant temperature heating plate 14 can be easily installed on the movable block 133.

[0029] refer to Figure 3The mounting bracket 9 has a screw hole 21 on its inner side. One end of the first hand-tightening bolt 12 is threaded through the screw hole 21. The mounting bracket 9 has a first sliding groove 22 symmetrically provided in its inner recess. The end of the clamping block 16 is slidably connected to the inner side of the first sliding groove 22. Through the screw hole 21 on the mounting bracket 9, the first hand-tightening bolt 12 can be threaded and extended on the mounting bracket 9. Through the first sliding groove 22 on the mounting bracket 9, the clamping block 16 can slide on the mounting bracket 9.

[0030] refer to Figure 4 The inner side of the mounting cavity 111 is symmetrically provided with a third sliding groove 27. The movable plate 115 and the third sliding groove 27 are slidably connected. A sliding hole 28 is provided on one side of the inner wall of the mounting cavity 111. One end of the pull rod 112 slides through the sliding hole 28. Through the third sliding groove 27 in the mounting cavity 111, the movable plate 115 can slide in the mounting cavity 111. Through the sliding hole 28 on the mounting cavity 111, one end of the pull rod 112 can slide through the inner wall of the mounting cavity 111.

[0031] Operating principle and advantages: When installing and connecting the mounting rod 10 and mounting bracket 9 on the support rod 7, the mounting rod 10 is placed on one side of the support rod 7, and the mounting bracket 9 is inserted from the other side of the mounting rod 10. When the connecting block 19 on the mounting rod 10 is inserted into the connecting groove 18, one end of the connecting block 19 presses against the inclined surface of the locking block 116, so that the movable plate 115 connected to the locking block 116 slides and presses the spring 114 in the mounting cavity 111. When the locking end of the locking block 116 corresponds to the locking hole 20 of the connecting block 19, the spring 114 resets and pushes the movable plate 115 to move, so that the locking block 116 on the movable plate 115 is inserted into the locking hole 20 and fixed. Subsequently, after connecting the mounting rod 10 and mounting bracket 9 at the support rod 7, the first hand-tightening bolt 12 is rotated. The thread of the first hand-tightening bolt 12 extends and retracts, pushing the clamping block 16 to slide. Since the clamping block 16 and the mounting rod 10 have a V-shaped groove 17 on their opposite sides, they can be quickly installed and fixed on the support rod 7.

[0032] During use, the hanging hole on the blood bag is hung onto the hook 701 of the support rod 7. When clamping the blood bag, simply use the handle to rotate the bidirectional screw 134 in the mounting groove 132 of the mounting rod 10. The rotation of the bidirectional screw 134 causes the two moving blocks 133 connected by the outer thread to slide closer or further apart. When they move away from each other, the blood bag is placed between the two constant temperature heating plates 14 connected to the moving blocks 133. When they move closer together, the clamping can be adjusted according to the condition of the blood bag.

Claims

1. A stem cell isolation and detection device, characterized by, The device includes a substrate, a centrifuge, a flow cytometer, a mounting frame, and mounting rods. Both the centrifuge and the flow cytometer are fixedly mounted on the upper end of the substrate. A centrifuge tube is located on the inner side of the upper end of the centrifuge. A support rod is fixedly mounted on the upper end of the centrifuge, and a hook is provided at the upper end of the support rod. The mounting rod is located on the front side of the support rod, and the mounting frame is located on the back side of the support rod. Connecting blocks are symmetrically fixedly mounted on the back side of the mounting rod. Connecting slots are symmetrically formed on the front side of the mounting frame. The mounting rods are inserted into the connecting blocks and connecting slots. A locking hole is formed on the inner side of each connecting block. The mounting bracket is connected by a connecting mechanism and a snap-fit ​​hole. A first hand-tightening bolt is threaded on the inner side of the back of the mounting bracket. The threaded end of the first hand-tightening bolt passes through the wall of the mounting bracket and is rotatably connected to a clamping block. The clamping block is slidably connected to the inner recess of the mounting bracket. V-shaped grooves are opened on the opposite surfaces of the clamping block and the mounting rod. A clamping mechanism is provided on the front of the mounting rod. A second hand-tightening bolt is provided on the outer side of the clamping mechanism. A constant temperature heating plate is fixedly installed on the clamping mechanism by the second hand-tightening bolt. An indicator light is provided on the outer side of the constant temperature heating plate. The clamping mechanism includes a mounting groove, which is formed on the front of the mounting rod. A bidirectional screw is rotatably connected to the inner side of the mounting groove. One end of the bidirectional screw passes through the inner wall of the mounting groove and is fixedly installed with a rotating block. A movable block is threadedly connected to the outer side of the bidirectional screw. One end of the movable block is slidably connected to the inner side of the mounting groove. The movable block is fixedly installed by a second hand-tightened bolt and a constant temperature heating plate.

2. The stem cell isolation and detection device of claim 1, wherein: The inner side of the mounting groove is symmetrically provided with a second sliding groove, and one end of the moving block and the second sliding groove are slidably connected.

3. The stem cell isolation and detection device of claim 1, wherein: The movable block has a mating groove on the side away from the mounting groove, and the mating block is fixedly installed on the outer side of the constant temperature heating plate. The mating block and the mating groove are connected by an insertion. The movable block is fixedly installed with the mating block by a second hand-tightened bolt.

4. The stem cell isolation and detection device of claim 1, wherein: The mounting bracket has a screw hole on its inner side, and one end of the first hand-tightening bolt is threaded through the screw hole. The mounting bracket has a first sliding groove symmetrically formed in its inner recess, and the end of the clamping block is slidably connected to the inner side of the first sliding groove.

5. The stem cell isolation and detection device of claim 1, wherein: The connecting mechanism includes a mounting cavity symmetrically located inside the mounting frame. A movable plate is slidably connected to the inner side of the mounting cavity. A locking block is fixedly installed on one side of the movable plate. The locking end of the locking block extends into the inner side of the connecting groove and engages with the locking hole of the connecting block. A pull rod is fixedly installed on the side of the movable plate away from the locking block. A pull ring is fixedly installed at the end of the pull rod away from the movable plate, which slides through the inner wall of the mounting cavity. A spring is sleeved on the outer side of the pull rod.

6. The stem cell isolation and detection device of claim 5, wherein: The inner side of the mounting cavity is symmetrically provided with a third sliding groove, and the movable plate and the third sliding groove are slidably connected.

7. The stem cell isolation and detection device of claim 5, wherein: A sliding hole is provided on one side of the inner wall of the mounting cavity, and one end of the pull rod slides through the sliding hole.

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