Exosome purification device

Abstract

The utility model provides an exosome purification device and belongs to the technical field of purification devices. Comprising a box body, a tempered glass window is arranged on the outer wall of the box body, a pipeline penetrates through the top of the box body and is connected with an infusion pump, a rotating rod penetrates through the inner wall of the pipeline, and the end, located on the outer wall of the pipeline, of the rotating rod is fixedly sleeved with a rotating disc. Through the arrangement of the pipeline, the infusion pump, the telescopic pipe, the positioning plate, the fixing plate and the telescopic plate structure, in the operation process of the device, a user can enable a gear to be meshed with the inner wall of a tooth groove by rotating a rotary disc, and therefore the height of the telescopic pipe can be adjusted; and meanwhile, a second electric telescopic rod is matched so that the height of a clamping strip can always correspond to the position of one tooth groove, it is guaranteed that the position of the telescopic pipe can be automatically locked after a user adjusts the height of the telescopic pipe, and then the automation and practicability of the device are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, in particular to an exosome purification device. Background Art

[0002] Exosomes were originally considered as the "garbage bags" of cells, allowing cells to get rid of some useless proteins. However, in recent years of research, it has been found that the "cargo" carried by exosomes has important biological significance. Therefore, there is still research value in modern society. In order to ensure the research progress, it is necessary to purify exosomes for observing their specific biological principles.

[0003] The existing purification equipment can refer to the Chinese utility model patent with the authorization announcement number of CN217795009U. It discloses a purification device for exosomes promoting wound healing, including a separation cylinder and a purification box. An installation seat is arranged on the outer side of the separation cylinder. A motor is installed at the bottom of the groove opened inside the installation seat. The output shaft of the motor is fixedly connected to the bottom of the separation cylinder. A telescopic assembly is arranged at the upper end of the separation cylinder. The upper end of the telescopic assembly is fixedly connected to a pipeline. A pump is arranged on the outer surface of the pipeline. By arranging a motor at the lower end of the separation cylinder and a telescopic assembly at the upper end of the separation cylinder, the separation cylinder is rotated by starting the motor, so that the stock solution contained in the separation cylinder can perform centrifugal motion to purify exosomes in the first step. Then, through the mutual cooperation of the telescopic assembly and the pump, exosomes are extracted and then enter the interior of the purification box through the pipeline, and are sequentially purified through a first filter plate, a second filter plate, and a third filter plate.

[0004] During the use of the above technical solution, although it can achieve a purification effect, the height of the liquid extraction pipeline cannot be adjusted during the operation process, so the use requirements of separation cylinders of different sizes cannot be met. Content of the Utility Model

[0005] The technical problem to be solved by the utility model is to provide an exosome purification device, which can automatically lock the position of the telescopic pipe after adjusting the height of the telescopic pipe and is convenient for cleaning the purification box.

[0006] To solve the above technical problems, the utility model provides the following technical solutions:

[0007] An exosome purification device, comprising a box body, the outer wall of the box body is provided with a tempered glass window, the top of the box body is penetrated by a pipeline, and the pipeline is connected to a liquid extraction pump; a rotating rod penetrates through the inner wall of the pipeline, and one end of the rotating rod located outside the pipeline is fixedly sleeved with a turntable, and the other end of the rotating rod is fixedly sleeved with a gear; the outer wall of the gear is engaged with a telescopic tube, the outer wall of the telescopic tube is fixedly sleeved with a sealing plate, a magnet is arranged on the outer wall of the telescopic tube, and a positioning plate is fixedly assembled on the outer wall of the pipeline; a fixing plate is also fixedly assembled on the outer wall of the pipeline, a second electric telescopic rod is arranged at the end of the fixing plate, the output shaft of the second electric telescopic rod is fixedly assembled with a telescopic plate, a second motor is fixedly assembled at the end of the telescopic plate, the output shaft of the second motor is fixedly sleeved with a second lead screw, a positioning block is threadedly connected to the outer wall of the second lead screw, a clamping strip is fixedly assembled on the top of the positioning block, and a magnetic induction sensor is fixedly assembled at the end of the clamping strip;

[0008] A housing is placed on the right side of the box body, a first motor is fixedly assembled on the outer wall of the housing, the output shaft of the first motor is fixedly sleeved with a first lead screw, a moving seat is threadedly connected to the outer wall of the first lead screw, a first electric telescopic rod is fixedly assembled on the top of the moving seat, and the output shaft of the first electric telescopic rod is fixedly assembled with a purification box;

[0009] A docking groove is opened on the outer wall of the purification box, a connecting plate is fixedly assembled on the inner wall of the docking groove, an ultrafiltration membrane is fixedly assembled at one end of the connecting plate close to the docking groove, and a long groove is opened on the inner wall of the housing.

[0010] A short groove is opened on the outer wall of the telescopic plate, and the inner wall of the short groove is slidably connected to the outer wall of the positioning block. A controller is fixedly assembled on the top of the box body, and the controller is electrically connected to the liquid extraction pump, the second motor, the second electric telescopic rod, the first electric telescopic rod, the first motor and the magnetic induction sensor respectively. A tooth groove is opened on the outer wall of the telescopic tube, and the inner wall of the tooth groove is engaged with the outer wall of the gear. The outer wall of the magnet is fixedly assembled with the inner wall of the tooth groove. A discharge pipe is arranged on the outer wall of the housing.

[0011] A square groove is opened at one end of the fixing plate away from the pipeline, and the inner wall of the square groove is slidably connected to the outer wall of the telescopic plate.

[0012] An installation groove is opened on the outer wall of the box body, and the inner wall of the installation groove is fixedly assembled with the outer wall of the tempered glass window. A separation cylinder and a centrifuge are arranged inside the box body, and the position of the tempered glass window corresponds to the position of the separation cylinder.

[0013] The inner wall of the long groove is slidably connected to the outer wall of the moving seat. The output shaft of the first electric telescopic rod extends 5 cm and then the top of the purification box is connected to the end of the pipeline away from the box body. Conversely, there is a 5 cm spacing between the purification box and the pipeline.

[0014] The connecting plate is fixedly connected to the purification box through fixing bolts. The number of ultrafiltration membranes is three, and the three ultrafiltration membranes are evenly distributed along the outer wall of the connecting plate.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] 1. Through the pipeline, liquid extraction pump, telescopic tube, positioning plate, fixing plate, and telescopic plate structures provided in the utility model, during the operation of the device, the user can rotate the turntable to make the gear engage with the inner wall of the tooth groove, thereby adjusting the height of the telescopic tube to ensure the extraction effect of the solution inside the separation cylinder of different sizes by the telescopic tube; at the same time, cooperating with the second electric telescopic rod can make the height of the clamping strip always correspond to the position of one tooth groove, ensuring that the user can automatically lock the position of the telescopic tube after adjusting the height of the telescopic tube.

[0017] 2. Through the shell, purification box, connecting plate, and ultrafiltration membrane structures provided in the utility model, the user can lift the purification box by the extension of the output shaft of the first electric telescopic rod, so that the solution can be completely introduced into the purification box to contact the ultrafiltration membrane, realizing the effect of hierarchical filtration; after filtration, the user can contract the output shaft of the first electric telescopic rod to make the purification box automatically move out of the shell, facilitating the user to remove the connecting plate and the ultrafiltration membrane, and further facilitating the user to clean and maintain the inside of the ultrafiltration membrane and the purification box. Description of the Drawings

[0018] Figure 1 It is a three-dimensional structural schematic diagram of the exosome purification device;

[0019] Figure 2 It is a structural schematic diagram of the purification box of the exosome purification device;

[0020] Figure 3 It is a structural schematic diagram of the shell of the exosome purification device;

[0021] Figure 4 It is a structural schematic diagram of the ultrafiltration membrane of the exosome purification device;

[0022] Figure 5 It is a structural schematic diagram of the rotating rod of the exosome purification device

[0023] Figure 6 It is a structural schematic diagram of the second electric telescopic rod of the exosome purification device.

[0024] [Reference Signs]

[0025] 1. Box body; 2. Tempered glass window; 3. Pipe; 4. Liquid extraction pump; 5. Shell; 6. Telescopic pipe; 7. Sealing plate; 8. Fixed plate; 9. Telescopic plate; 10. Purification box; 11. First motor; 12. First lead screw; 13. Moving seat; 14. First electric telescopic rod; 15. Connecting plate; 16. Long groove; 17. Ultrafiltration element; 18. Docking groove; 19. Positioning plate; 20. Turntable; 21. Gear; 22. Rotating rod; 23. Second electric telescopic rod; 24. Card strip; 25. Magnetic induction sensor; 26. Magnet; 27. Second motor; 28. Second lead screw; 29. Positioning block. Detailed implementation mode

[0026] The following combines the attached drawings and specific embodiments to describe in detail an exosome purification device provided by the present utility model. At the same time, it is hereby explained that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments. For some technicians in the field of well-known technologies, other alternative methods can also be adopted for implementation; and the attached drawings are only for more specifically describing the embodiments, and are not intended to specifically limit the present utility model.

[0027] See Figures 1-6 , an exosome purification device, comprising: a box body 1, a tempered glass window 2 is provided on the outer wall of the box body 1, a pipe 3 penetrates through the top of the box body 1, and the pipe 3 is connected to a liquid extraction pump 4; a rotating rod 22 penetrates through the inner wall of the pipe 3, a turntable 20 is fixedly sleeved at one end of the rotating rod 22 located on the outer wall of the pipe 3, a gear 21 is fixedly sleeved at the other end of the rotating rod 22, a telescopic pipe 6 is meshed with the outer wall of the gear 21, a sealing plate 7 is fixedly sleeved on the outer wall of the telescopic pipe 6, and a magnet 26 is provided on the outer wall of the telescopic pipe 6;

[0028] A positioning plate 19 is fixedly assembled on the outer wall of the pipe 3, a fixed plate 8 is also fixedly assembled on the outer wall of the pipe 3, a second electric telescopic rod 23 is provided at the end of the fixed plate 8, a telescopic plate 9 is fixedly assembled on the output shaft of the second electric telescopic rod 23, a second motor 27 is fixedly assembled at the end of the telescopic plate 9, a second lead screw 28 is fixedly sleeved on the output shaft of the second motor 27, a positioning block 29 is threadedly connected to the outer wall of the second lead screw 28, a card strip 24 is fixedly assembled on the top of the positioning block 29, and a magnetic induction sensor 25 is fixedly assembled at the end of the card strip 24;

[0029] On the right side of the box body 1, there is a shell 5 placed. A first motor 11 is fixedly assembled on the outer wall of the shell 5. A first lead screw 12 is fixedly sleeved on the output shaft of the first motor 11. A moving seat 13 is threadedly connected to the outer wall of the first lead screw 12. A first electric telescopic rod 14 is fixedly assembled on the top of the moving seat 13. A purification box 10 is fixedly assembled on the output shaft of the first electric telescopic rod 14. A docking groove 18 is opened on the outer wall of the purification box 10. A connecting plate 15 is fixedly assembled on the inner wall of the docking groove 18. A ultrafiltration element 17 is fixedly assembled at one end of the connecting plate 15 close to the docking groove 18. A long groove 16 is opened on the inner wall of the shell 5.

[0030] In the above structure, through the structures of the pipeline 3, the telescopic pipe 6, the rotating rod 22, the positioning plate 19, and the gear 21, when the user needs to adjust the height of the telescopic pipe 6 to facilitate the user to extract the solution inside the separation cylinders of different sizes, the user can control the second motor 27 to operate in the reverse direction through the controller, so that the clamping strip 24 disengages from the inner wall of the tooth groove, thereby making the telescopic pipe 6 in a movable state, and then adjusting the height of the telescopic pipe 6 by rotating the turntable 20, so that the telescopic pipe 6 can extract the solution in the separation cylinders of different sizes; and through the docking of the clamping strip 24 and the tooth groove, the telescopic pipe 6 cannot shake or even shift in position during the extraction process, improving the stability of the telescopic pipe 6 during the operation.

[0031] In a preferred embodiment, short grooves are opened on the outer wall of the telescopic plate 9. The inner wall of the short grooves is slidably connected to the outer wall of the positioning block 29. A controller is fixedly assembled on the top of the box body 1. The controller is electrically connected to the liquid extraction pump 4, the second motor 27, the second electric telescopic rod 23, the first electric telescopic rod 14, the first motor 11, and the magnetic induction sensor 25 respectively. Tooth grooves are opened on the outer wall of the telescopic pipe 6. The inner wall of the tooth grooves is meshed with the outer wall of the gear 21. A magnet 26 is fixedly assembled on the inner wall of the tooth grooves. A discharge pipe is provided on the outer wall of the shell 5.

[0032] In the above structure, through the structures of the short grooves and the positioning block 29, the user can adjust the heights of both the telescopic plate 9 and the clamping strip 24 through the movement of the output shaft of the second electric telescopic rod 23. During the movement of the telescopic pipe 6, the position of its tooth grooves will also shift. The movability of the telescopic plate 9 cooperates with the induction of the position of the magnet 26 by the magnetic induction sensor 25 to ensure that the clamping strip 24 can be aligned with the tooth grooves, enabling the clamping strip 24 to be accurately docked with the inner wall of the tooth grooves, thereby achieving the effect of locking the telescopic pipe 6 to ensure the stability of the telescopic pipe 6 during the operation.

[0033] In a preferred embodiment, a square groove is opened at one end of the fixing plate 8 away from the pipeline 3. The inner wall of the square groove is slidably connected to the outer wall of the telescopic plate 9.

[0034] In the above structure, by setting the square groove and the telescopic plate 9 structure, when the output shaft of the second electric telescopic rod 23 moves, it can drive the telescopic plate 9 to move up and down, and the square groove can provide a movable space for the movement of the telescopic plate 9, and cooperate with the magnetic sensor 25 to sense the position of the magnet 26, so that the device can automatically correct the height of the telescopic plate 9 and make the card strip 24 contact with the inner wall of the tooth groove, thereby improving the automation and intelligence of the device.

[0035] In a preferred embodiment, a mounting groove is provided on the outer wall of the box body 1, and the inner wall of the mounting groove is fixedly assembled with the outer wall of the tempered glass window 2. A separation cylinder and a centrifuge are provided on the inner wall of the box body 1, and the position of the tempered glass window 2 corresponds to the position of the separation cylinder.

[0036] In the above structure, by setting up the installation groove structure, the user can observe through the tempered glass window 2 whether the bottom end of the telescopic tube 6 contacts the bottom of the separation cylinder, so as to ensure the amount of solution extracted by the device and avoid the situation where the telescopic tube 6 cannot extract the solution at its bottom during the operation of the equipment.

[0037] In a preferred embodiment, the inner wall of the long groove 16 is slidably connected to the outer wall of the movable seat 13. After the output shaft of the first electric telescopic rod 14 extends 5 cm, the top of the purification box 10 is connected to the end of the pipeline 3 away from the box body 1. Conversely, there is a 5 cm distance between the purification box 10 and the pipeline 3.

[0038] In the above structure, the connection state between the purification box 10 and the pipeline 3 is adjusted by extending and contracting the output shaft of the first electric telescopic rod 14, so that the user can use the output shaft of the first electric telescopic rod 14 to lift the purification box 10 after moving the connecting plate 15 to the inside of the shell 5, so that the pipeline 3 is connected to the top of the purification box 10, ensuring that the pipeline 3 can transport the solution to the inside of the purification box 10, and use the ultrafiltration element 17 to perform graded filtration operations to achieve a purification effect;

[0039] After the output shaft of the first electric telescopic rod 14 is retracted, the purification box 10 and the pipeline 3 are in a separated state. The user can drive the first screw 12 to rotate by the first motor 11, so as to move the purification box 10 out of the shell 5, so that the user can remove the connecting plate 15 conveniently; at the same time, take out the three ultrafiltration elements 17 to clean the ultrafiltration elements 17 and the inside of the purification box 10, thereby improving the user's activity space and the maintenance efficiency of the device.

[0040] In a preferred embodiment, the connecting plate 15 is fixedly connected to the purification box 10 by fixing bolts, and the number of ultrafiltration elements 17 is three, and the three ultrafiltration elements 17 are evenly distributed along the outer wall of the connecting plate 15 .

[0041] In the above structure, through the three ultrafiltration element 17 structures provided, after the solution enters the purification tank 10, the three ultrafiltration elements 17 are used to perform a grading filtration operation on the solution, thereby improving the purification effect of the device on exosomes.

[0042] The working principle is as follows. First, after the user assembles the device, the separation cylinder is placed on the centrifuge. Subsequently, the height of the telescopic tube 6 can be increased by rotating the turntable 20. When adjusted until the bottom of the telescopic tube 6 contacts the inner wall of the bottom of the separation cylinder, the magnetic sensor 25 will sense the magnet 26;

[0043] When a dislocation occurs, the controller controls the output shaft of the second electric telescopic rod 23 to operate, so that the position of the magnetic sensor 25 corresponds to the position of the magnet 26. Then, the output shaft of the second motor 27 drives the second lead screw 28 to rotate, so that the magnetic sensor 25 fits with the magnet 26. At this time, the telescopic tube 6 cannot move under the limiting action of the clamping strip 24, ensuring the extraction of the solution by the telescopic tube 6;

[0044] The solution is transported to the purification tank 10 through the cooperation of the liquid extraction pump 4 and the pipeline 3, and undergoes a grading filtration operation by the three ultrafiltration elements 17. The user directly collects the obtained product through the discharge pipe;

[0045] After the filtration is completed, the user can control the output shaft of the first electric telescopic rod 14 to contract through the controller, so that the purification tank 10 is separated from the pipeline 3. Then, the output shaft of the first motor 11 drives the first lead screw 12 to rotate, so that the purification tank 10 moves out of the housing 5. Subsequently, the fixing bolts are removed, and then the connecting plate 15 together with the ultrafiltration element 17 is removed. Finally, the three ultrafiltration elements 17 and the inside of the purification tank 10 are cleaned.

[0046] The above is only the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art in this technical field, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. An exosome purification device, characterized in that: The invention comprises a box body (1), wherein the outer wall of the box body (1) is provided with a tempered glass window (2), the top of the box body (1) is penetrated by a pipe (3), and the pipe (3) is connected to a liquid pump (4); the inner wall of the pipe (3) is penetrated by a rotating rod (22), one end of the rotating rod (22) located on the outer wall of the pipe (3) is fixedly sleeved with a rotating disk (20), and the other end of the rotating rod (22) is fixedly sleeved with a gear (21); the outer wall of the gear (21) is meshed with a telescopic tube (6), the outer wall of the telescopic tube (6) is fixedly sleeved with a sealing plate (7), the outer wall of the telescopic tube (6) is provided with a magnet (26), and the outer wall of the pipe (3) is fixedly sleeved with a sealing plate (7). A positioning plate (19) is fixedly mounted on the outer wall of the pipe (3); a fixing plate (8) is also fixedly mounted on the outer wall of the pipe (3); a second electric telescopic rod (23) is provided at the end of the fixing plate (8); the output shaft of the second electric telescopic rod (23) is fixedly mounted on the telescopic plate (9); a second motor (27) is fixedly mounted on the end of the telescopic plate (9); a second screw rod (28) is fixedly sleeved on the output shaft of the second motor (27); a positioning block (29) is threadedly connected to the outer wall of the second screw rod (28); a clamping strip (24) is fixedly mounted on the top of the positioning block (29); a magnetic sensor (25) is fixedly mounted on the end of the clamping strip (24); A shell (5) is placed on the right side of the box body (1), a first motor (11) is fixedly mounted on the outer wall of the shell (5), a first screw rod (12) is fixedly sleeved on the output shaft of the first motor (11), a movable seat (13) is threadedly connected to the outer wall of the first screw rod (12), a first electric telescopic rod (14) is fixedly mounted on the top of the movable seat (13), and a purification box (10) is fixedly mounted on the output shaft of the first electric telescopic rod (14); The outer wall of the purification box (10) is provided with a docking groove (18), the inner wall of the docking groove (18) is fixedly equipped with a connecting plate (15), an end of the connecting plate (15) close to the docking groove (18) is fixedly equipped with an ultrafiltration element (17), and the inner wall of the shell (5) is provided with a long groove (16).

2. The exosome purification device according to claim 1, characterized in that: The outer wall of the telescopic plate (9) is provided with a short groove, the inner wall of the short groove is slidably connected to the outer wall of the positioning block (29), the top of the box body (1) is fixedly equipped with a controller, and the controller is electrically connected to the liquid pump (4), the second motor (27), the second electric telescopic rod (23), the first electric telescopic rod (14), the first motor (11) and the magnetic sensor (25) respectively; the outer wall of the telescopic tube (6) is provided with a tooth groove, the inner wall of the tooth groove is meshed with the outer wall of the gear (21), the outer wall of the magnet (26) is fixedly assembled with the inner wall of the tooth groove, and the outer wall of the shell (5) is provided with a discharge pipe.

3. The exosome purification device according to claim 1, characterized in that: A square groove is formed at one end of the fixed plate (8) away from the pipeline (3), and the inner wall of the square groove is slidably connected to the outer wall of the telescopic plate (9).

4. The exosome purification device according to claim 1, characterized in that: The outer wall of the box body (1) is provided with a mounting groove, the inner wall of the mounting groove is fixedly assembled with the outer wall of the tempered glass window (2), the inner wall of the box body (1) is provided with a separation cylinder and a centrifuge, and the position of the tempered glass window (2) corresponds to the position of the separation cylinder.

5. The exosome purification device according to claim 1, characterized in that: The inner wall of the long groove (16) is slidably connected to the outer wall of the movable seat (13); after the output shaft of the first electric telescopic rod (14) is extended by 5 cm, the top of the purification box (10) is connected to the end of the pipeline (3) away from the box body (1); otherwise, there is a 5 cm gap between the purification box (10) and the pipeline (3).

6. The exosome purification device according to claim 1, characterized in that: The connecting plate (15) is fixedly connected to the purification box (10) by means of fixing bolts. The number of the ultrafiltration elements (17) is three, and the three ultrafiltration elements (17) are evenly distributed along the outer wall of the connecting plate (15).

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