Hematopoietic stem cell colony culture device

Abstract

The utility model relates to the technical field of colony culture, in particular to a hematopoietic stem cell colony culture device which comprises a box body, a culture plate cover arranged at the upper end of the box body, a culture plate bottom arranged at the lower end of the box body, and a first culture pond and a second culture pond arranged on the culture plate bottom, moisturizing pools are arranged in the outer walls of the first culture pool and the second culture pool and the periphery of the bottom of the culture plate, the outer walls of the first culture pool and the second culture pool are communicated with the moisturizing pools, and a water injection hole is formed in the connecting center of the horizontal upper positions of the first culture pool and the second culture pool; a ventilating cover is arranged at the central position where the lower parts of the first culture pond and the second culture pond are connected, and the ventilating cover is vertically aligned with the water injection hole; through the design that the outer walls of the first culture pond and the second culture pond are communicated with the inner surface of the bottom of the culture plate and the water injection holes are additionally arranged, the fringe effect of evaporation of edge hole sites is reduced when the hematopoietic stem cell colonies are cultured, and meanwhile water adding operation is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of colony culture, and specifically relates to a hematopoietic stem cell colony culture device. Background Art

[0002] Hematopoietic stem cells are a group of pluripotent stem cells with self-renewal ability and multi-lineage differentiation potential. They can not only maintain the stability of their own state and function, but also differentiate into different types of hematopoietic progenitor cells, and further differentiate into functional blood cell types of various lineages. They can reconstruct the human hematopoietic system and immune system. A hematopoietic stem cell colony refers to a cell mass formed by the proliferation of an ancestor cell in an in vitro culture medium. During the hematopoietic stem cell colony culture experiment, petri dishes or culture plates are often used for colony culture, which is a commonly used and important tool for culture, and their specifications and types are diverse.

[0003] For the existing petri dish, three small petri dishes with the same diameter are required during culture. Two of them are respectively filled with hematopoietic stem cell culture suspension, and the other is filled with sterile water. They are evenly placed in a large-diameter petri dish and then placed in a carbon dioxide incubator for culture. During the culture process, it is easily affected by movement and collision. Moreover, when observing the culture condition of hematopoietic stem cells in the petri dish, the petri dish needs to be taken out for observation, and the microorganisms attached to the operator's hands may affect the culture and differentiation of hematopoietic stem cells. When the existing six-well plate is used for dynamic culture of hematopoietic stem cells, an appropriate amount of cell suspension needs to be added inside the six-well plate. However, during the culture process, the sealing performance of the six-well plate is not good, and the evaporation efficiency of the edge wells is too high, affecting the cell growth rate. If a batch of hematopoietic stem cell colony culture experiments are carried out using a six-well plate for culture, in order to ensure the accuracy of the test results, generally the middle two wells are selected for colony culture, which will result in the idleness of 4 culture wells, causing waste of resources and funds. Moreover, the culture plate lacks a signature place and a place for marking cell numbers. If marked on the extra well plate with a marker pen, it is easy to cause clerical errors. Since multiple people share a carbon dioxide incubator, it is sometimes inevitable that the opening and closing of the door will cause collision and vibration to the culture device in the incubator. For the existing six-well plate, when observing the hematopoietic stem cell colony culture state, a large amount of water mist will appear on the inner side of the culture plate cover, affecting the observation results. Therefore, a hematopoietic stem cell colony culture device is needed to improve the above problems. Summary of the Utility Model

[0004] In the process of cell culture, the sealing performance of the six-well plate is not good, and the evaporation efficiency of the wells at the edge is too high, which affects the cell growth rate. If a batch of hematopoietic stem cell colony culture experiments are carried out using a six-well plate for culture, in order to ensure the accuracy of the test results, generally the two middle wells are selected for colony culture, which will result in the idleness of 4 culture wells, causing waste of resources and funds. The purpose of the present utility model is to provide a hematopoietic stem cell colony culture device to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present utility model provides the following technical solutions:

[0006] A hematopoietic stem cell colony culture device includes a box body, a culture plate cover is arranged at the upper end of the box body, a culture plate bottom is arranged at the lower end of the box body, a first culture pool and a second culture pool are arranged on the culture plate bottom, a moisturizing pool is arranged between the outer walls of the first culture pool and the second culture pool and the periphery of the culture plate bottom, the outer walls of the first culture pool and the second culture pool are communicated with the moisturizing pool, a water injection hole is arranged at the center of the connection position above the horizontal plane of the first culture pool and the second culture pool, and a breathable cover is arranged at the center position of the connection position below the first culture pool and the second culture pool, and the breathable cover is vertically aligned with the water injection hole;

[0007] A sealing rubber plug is arranged at the upper end of the water injection hole;

[0008] Anti-slip strips are fixedly installed on the side surfaces around the culture plate bottom.

[0009] As a preferred solution of the present utility model, the first culture pool and the second culture pool are circular hole pools with the same diameter, the diameter is 35mm, and there is no meniscus setting.

[0010] As a preferred solution of the present utility model, the outer walls of the first culture pool and the second culture pool are communicated with the moisturizing pool around the periphery.

[0011] As a preferred solution of the present utility model, a water injection hole is arranged at the upper middle position of the culture plate cover, and a sealing rubber plug is arranged at the upper end of the water injection hole.

[0012] As a preferred solution of the present utility model, a barcode area is arranged on the left side below the culture plate cover, and a signature area is arranged on the right side below the culture plate cover, and the signature area is made of frosted material.

[0013] As a preferred solution of the present utility model, inner grooves are added at the four corners of the culture plate bottom, and T-shaped silica gel plugs can be placed inside the grooves.

[0014] As a preferred solution of the present utility model, an air-permeable cover is provided at the central position where the lower positions of the first culture pool and the second culture pool are connected. The inner part of the air-permeable cover is provided with a screw thread and is made of PP material, that is, polypropylene material. An air-permeable membrane is provided inside the port of the air-permeable cover, which is a hydrophobic filter membrane with a pore diameter of 0.22 um. It can meet the requirements of gas exchange in cell culture and can effectively prevent cross-contamination, and is suitable for open culture conditions.

[0015] As a preferred solution of the present utility model, a sealing rubber plug made of silica gel and resistant to high temperature is provided at the upper end of the water injection hole.

[0016] As a preferred solution of the present utility model, both the bottom of the culture plate and the culture plate cover are made of transparent PS, that is, transparent polystyrene material; the surfaces of the first culture pool and the second culture pool are TC-treated, and the culture device is sterilized before use.

[0017] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0018] 1. In the present utility model, by utilizing the interconnection between the outer walls of the first culture pool and the second culture pool and the inner surface of the bottom of the culture plate and the design of adding a water injection hole, when culturing hematopoietic stem cell colonies, the edge effect of evaporation at the edge holes is reduced, and at the same time, the water addition operation is convenient.

[0019] 2. In the present utility model, by adding an air-permeable cover to culture hematopoietic stem cell colonies, the microbial contamination caused by the external environment is greatly reduced. By adding anti-slip settings at the bottom and side, the impact of collision and vibration on the culture device caused by multiple door openings and closings in the culture incubator shared by multiple people during long-term culture is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is a schematic diagram of the overall structure of the present utility model;

[0021] Figure 2 is a schematic diagram of the internal structure of the present utility model;

[0022] Figure 3 is a schematic diagram of the structure of the culture component of the present utility model;

[0023] Figure 4 is a schematic diagram of the structure of the top component of the plate cover of the present utility model.

[0024] In the figure: 1, bottom of the culture plate; 2, culture plate cover; 3, water injection hole; 4, first culture pool; 5, moisture preservation pool; 6, bar code area; 7, signature area; 8, air-permeable cover; 9, second culture pool. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0025] Next, in combination with the embodiments of the present utility model, the technical solutions in the embodiments of the present utility model will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0026] Embodiment: Please refer to Figures 1 - 4 A hematopoietic stem cell colony culture device as shown, which includes a box body. A culture plate cover 2 is provided at the upper end of the box body, and a culture plate bottom 1 is provided at the lower end of the box body. A first culture pool 4 and a second culture pool 9 are provided on the culture plate bottom 1. A moisture preservation pool 5 is provided between the outer walls of the first culture pool 4 and the second culture pool 9 and within the periphery of the culture plate bottom 1. The outer walls of the first culture pool 4 and the second culture pool 9 are in communication with the moisture preservation pool 5. At the position above the horizontal of the first culture pool 4 and the second culture pool 9, a water injection hole 3 is provided at the connecting center. At the center position connected to the lower part of the first culture pool 4 and the second culture pool 9, a ventilation cover 8 is provided. The ventilation cover 8 is vertically aligned with the water injection hole 3. A sealing rubber plug is provided at the upper end of the water injection hole 3. Anti-slip strips are fixedly installed at the peripheral side positions of the culture plate bottom 1. The design of the communication between the outer walls of the first culture pool 4 and the second culture pool 9 and the inner surface of the culture plate bottom 1 and the addition of the water injection hole 3 achieve the reduction of the edge effect of evaporation at the edge holes during the culture of hematopoietic stem cell colonies, and at the same time facilitate the water addition operation.

[0027] In this embodiment, referring to Figure 1 、 Figure 2 and Figure 3 As shown, the first culture pool 4 and the second culture pool 9 are circular hole pools with uniform diameters, the diameter is 35 mm, and there is no meniscus setting. The outer walls around the first culture pool 4 and the second culture pool 9 are in communication with the moisture preservation pool 5. A water injection hole 3 is provided at the upper middle position of the culture plate cover 2. A sealing rubber plug is provided at the upper end of the water injection hole 3. A barcode area 6 is provided on the left side below the culture plate cover 2, and a signature area 7 is provided on the right side below the culture plate cover 2. The signature area 7 is made of frosted material. By adding a ventilation cover 8 to culture hematopoietic stem cell colonies, the microbial contamination caused by the external environment is greatly reduced.

[0028] In this embodiment, referring to Figure 1 、 Figure 3 and Figure 4As shown, inner grooves are added at the four corners of the bottom 1 of the culture plate. T-shaped silicone plugs can be placed inside the grooves. An air-permeable cover 8 is provided at the central position where the bottom positions of the first culture pool 4 and the second culture pool 9 are connected. The inner part of the air-permeable cover 8 is provided with a screw thread and is made of PP material, that is, polypropylene material. A breathable membrane is provided inside the port of the air-permeable cover 8, which is a hydrophobic filter membrane with a pore size of 0.22um, which can meet the gas exchange requirements in cell culture and can effectively prevent cross-contamination, and is suitable for open culture conditions. A sealing rubber plug, made of silicone material and heat-resistant, is provided at the upper end of the water injection hole 3. The bottom 1 of the culture plate and the culture plate cover 2 are both made of transparent PS, that is, transparent polystyrene material; the surfaces of the first culture pool 4 and the second culture pool 9 are TC-treated, and the culture device is sterilized before use. By increasing the anti-slip settings at the bottom and sides, the impact of the collision and vibration of the culture device caused by multiple door openings and closings in the shared incubator during long-term culture is reduced.

[0029] In the use of a hematopoietic stem cell colony culture device in this solution, the hematopoietic stem cell colony culture device is placed in a biosafety cabinet. After checking its integrity, the culture plate cover 2 is opened and inverted on the desktop of the biosafety cabinet. After inoculating hematopoietic stem cell suspension into the first culture pool 4 and the second culture pool 9, sufficient sterile water is added to the moisturizing pool 5, and then the culture plate cover 2 is closed.

[0030] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A hematopoietic stem cell colony culture device, comprising a box body, characterized in that: A culture plate cover (2) is arranged at the upper end of the box body, and a culture plate bottom (1) is arranged at the lower end of the box body. A first culture pool (4) and a second culture pool (9) are arranged on the culture plate bottom (1). A moisturizing pool (5) is arranged within the outer walls of the first culture pool (4) and the second culture pool (9) and the culture plate bottom (1). The outer walls of the first culture pool (4) and the second culture pool (9) are connected to the moisturizing pool (5). A water injection hole (3) is arranged at the center of the connection between the first culture pool (4) and the second culture pool (9) at the horizontal upper position. A breathable cover (8) is arranged at the center of the connection between the lower position of the first culture pool (4) and the second culture pool (9). The breathable cover (8) is kept vertically aligned with the water injection hole (3). A sealing rubber plug is provided at the upper end of the water injection hole (3); Anti-slip strips are fixedly installed on the sides of the culture plate bottom (1).

2. A hematopoietic stem cell colony culture device according to claim 1, characterized in that: The first culture pool (4) and the second culture pool (9) are circular hole pools with uniform diameters, with a diameter of 35 mm and are arranged without a meniscus.

3. A hematopoietic stem cell colony culture device according to claim 1, characterized in that: The outer walls of the first culture pool (4) and the second culture pool (9) are interconnected with the moisturizing pool (5) on all sides.

4. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: A water injection hole (3) is provided at the upper middle position of the culture plate cover (2), and a sealing rubber plug is provided at the upper end of the water injection hole (3).

5. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: A barcode area (6) is arranged on the left side below the culture plate cover (2), and a signature area (7) is arranged on the right side below the culture plate cover (2), wherein the signature area (7) is made of frosted material.

6. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: Inner grooves are provided at the four corners of the culture plate bottom (1).

7. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: A breathable cover (8) is provided at the central position where the lower positions of the first culture tank (4) and the second culture tank (9) are connected. The breathable cover (8) has a screw thread inside and is made of PP material, i.e., polypropylene. A breathable membrane is provided inside the port of the breathable cover (8), which is a hydrophobic filter membrane with a pore size of 0.22 um.

8. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: The upper end of the water injection hole (3) is provided with a sealing rubber plug made of silica gel and resistant to high temperatures.

9. The hematopoietic stem cell colony culture device according to claim 1, characterized in that: The culture plate bottom (1) and the culture plate cover (2) are both made of transparent PS, i.e. transparent polystyrene; the surfaces of the first culture pool (4) and the second culture pool (9) are TC treated.

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