Brucellosis tiger red plate agglutination test device

Abstract

The Brucellosis Huhong plate agglutination test device comprises a reaction substrate and a stirring comb. The reaction substrate is integrally molded from a milky opaque plastic, has a reaction front surface and a support back surface, in which an array of blind holes is formed, each blind hole defining a circular opening in the reaction front surface of the reaction substrate and defining an inner circumferential wall and a circular bottom in a thickness direction of the reaction substrate, wherein the diameter of the inner circumferential wall of each blind hole is gradually reduced from the circular opening to the circular bottom, the diameter of the circular opening is 19-21 mm, and the diameter of the circular bottom is 15-17 mm. The stirring comb is provided with at least one row of parallel stirring needles, and the stirring needles correspond to at least one row of blind holes in the blind hole array respectively so that the stirring needles can stretch into the blind holes in a stirring mode. According to the Brucellosis Tianhong plate agglutination test device disclosed by the utility model, multiple samples can be detected at the same time, the mutual pollution and interference among the samples are effectively avoided, and meanwhile, the samples are ensured to have enough flowability within the test specified result interpretation time, so that the accurate detection can be carried out.

Description

Technical Field

[0001] The utility model relates to a plate agglutination test device. Background Art

[0002] The detection method with the highest frequency of use in the diagnosis of brucellosis is the brucellosis rose bengal plate agglutination test, which is also the designated test for the detection of brucellosis in cattle, sheep and pigs in international trade. This detection method has the advantages of low cost of test reagents, simple operation, rapidity, high detection rate, etc., and is widely used in large-scale screening of brucellosis, introduction of breeding stock, and evaluation of vaccine immunization effects, etc.

[0003] During detection, the sample to be tested is mixed evenly with the brucellosis rose bengal plate agglutination test antigen reagent on a clean glass slide or plate, and it is observed within 4 minutes whether visible particles or flocculent agglutination are formed. If particles or flocculent agglutination appear in the mixed solution, it indicates that an agglutination reaction has occurred, indicating that the sample to be tested is positive; if no particles or flocculent agglutination phenomenon appears, it indicates that no agglutination reaction has occurred, indicating that the sample to be tested is negative. However, when multiple samples are detected on the same glass slide or plate, it is easy to cause problems such as mutual contamination between samples, visual interference, and inconsistent reaction speeds caused by inconsistent interfacial tensions of the reaction plate, thereby affecting the accuracy of the experimental results. Content of the Utility Model

[0004] The purpose of the utility model is to provide a brucellosis rose bengal plate agglutination test device that is convenient for accurate simultaneous detection of multiple samples.

[0005] The brucellosis rose bengal plate agglutination test device according to the utility model includes:

[0006] A reaction substrate, integrally molded from a milky white opaque resin, having a reaction front surface and a supporting back surface, in which an array of blind holes is formed. Each blind hole defines a circular opening on the reaction front surface of the reaction substrate, and defines an inner circumferential wall and a circular bottom in the thickness direction of the reaction substrate. The diameter of the inner circumferential wall of each blind hole gradually decreases from the circular opening to the circular bottom. The diameter of the circular opening is 19 - 21 mm, and the diameter of the circular bottom is 15 - 17 mm;

[0007] A stirring comb, formed with at least one row of mutually parallel stirring needles, respectively corresponding to at least one row of blind holes in the blind hole array so as to extend into them stir-ably.

[0008] In the brucellosis rose bengal plate agglutination test device according to the utility model, the blind hole array is composed of a plurality of uniformly distributed blind holes, and on the reaction front surface of the reaction substrate, corresponding hole position numbers are set at set positions near the circular openings of each blind hole.

[0009] According to the Brucella RBPT device of the present utility model, the depth of each blind hole is 1-3 mm, preferably about 2 mm, so as to provide sufficient space for a 60-μL reaction system.

[0010] According to the Brucella RBPT device of the present utility model, the reaction substrate is preferably made of a rigid hydrophilic modified ABS resin (acrylonitrile-butadiene-styrene copolymer). This is beneficial to reducing the solid-liquid interfacial tension, fully mixing the reactants, and promoting the agglutination reaction.

[0011] According to the Brucella RBPT device of the present utility model, the stirring comb is preferably integrally molded from soft polypropylene (PP).

[0012] The Brucella RBPT device of the present utility model can simultaneously perform multi-sample detection, effectively avoiding cross-contamination and interference between samples, and at the same time ensuring that the samples have sufficient fluidity within the specified result interpretation time of the test, so as to enable accurate detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 FIG. is a schematic cross-sectional structure diagram of the reaction substrate of the Brucella RBPT device according to the present utility model;

[0014] Figure 2 FIG. is a top view schematic diagram of the reaction substrate of the Brucella RBPT device according to the present utility model;

[0015] Figure 3 FIG. is a schematic structural diagram of the stirring comb of the Brucella RBPT device according to the present utility model. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] The present utility model will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0017] Figure 1 and Figure 2 are respectively a schematic cross-sectional structure diagram and a top view schematic diagram of the Brucella RBPT device according to the present utility model; Figure 3 FIG. is a schematic structural diagram of the stirring comb of the Brucella RBPT device according to the present utility model.

[0018] As Figures 1 - 3 shown, the Brucella RBPT device of the present utility model generally consists of a reaction substrate 10 and a stirring comb 30.

[0019] The reaction substrate 10 is integrally injection-molded from a milky white opaque resin to facilitate the observation of whether particle or flocculent agglutination occurs between the antigen reagent and the sample, as further described below. The resin can be a rigid hydrophilic modified ABS resin (acrylonitrile-butadiene-styrene copolymer) to facilitate the cleaning of the reaction substrate 10. The reaction substrate 10 has a reaction front side ( Figure 1 the upper surface) and a support back side ( Figure 1 the lower surface), and is placed on the test bench through the support back side.

[0020] A plurality of blind holes 20 are formed in the reaction substrate 10, which are uniformly distributed and form an array. Each blind hole 20 defines a circular opening 21 on the reaction front side of the reaction substrate 10, and defines an inner circumferential wall 22 and a circular bottom 23 along the thickness direction ( Figure 1 the up-and-down direction) of the reaction substrate 10. The diameter of the inner circumferential wall 22 of each blind hole 20 gradually decreases from the circular opening 21 to the circular bottom 23. The diameter of the circular opening 21 is about 20 mm, and the diameter of the circular bottom 23 is about 16 mm. The depth of each blind hole 20 is about 2 mm.

[0021] Although not specifically shown, on the reaction front side of the reaction substrate 10, corresponding hole position numbers can be set at set positions near the circular opening 21 of each blind hole 20, such as the upper left corner.

[0022] As Figure 3 shown, the stirring comb 30 is formed with a row of stirring needles 31 parallel to each other. These stirring needles 31 can respectively correspond to a row of blind holes 20 in the blind hole array and extend into them in a stirrable manner. The stirring comb 30 can also be integrally injection-molded, for example, made of soft polypropylene (PP); this material is not easy to form scratches, and the edge position can be in full contact with the reaction substrate, thus facilitating the full mixing of the reaction system. Although not specifically shown, the stirring comb 30 can also be formed with more rows of stirring needles 31 parallel to each other.

[0023] When performing multi-sample detection simultaneously, numbered samples can be placed into the blind holes 20 with corresponding hole position numbers, and antigen reagents (purple-red liquid) are added. Then, a stirring comb 30 is used to perform batch rapid stirring on a corresponding column of the blind hole array. By observing whether particles or flocculent agglutination occur in the samples and antigen reagents (also showing obvious purple-red) after the mixing reaction in the blind holes 20, it can be determined whether an agglutination reaction occurs between the antigen reagents and the samples. Since the stirring comb 30 integrated with multiple stirring needles 31 is used, the reaction is timely and rapid, thus minimizing the observation error of batch sample detection within the specified test time. Additionally, since the reaction substrate 10 uses an opalescent non-transparent matrix, the contrast is higher compared to traditional glass plates, enabling the experimenter to clearly observe whether particles or flocculent agglutination occur after the mixing of each sample and antigen reagent, especially for weakly positive samples, so that the test results of the samples can be determined more accurately, improving the detection sensitivity. Moreover, since the reaction substrate 10 uses a hydrophilic modified ABS resin, the reaction substrate 10 can be rinsed clean with clean water and is easy to clean, and can be reused.

[0024] In the present utility model, this concave and tapered structure of the blind hole 21 can effectively promote backflow, prevent accidental spillage of the content, avoid cross-contamination between adjacent blind holes, thereby improving the consistency and accuracy of the detection results.

[0025] In addition, in the present utility model, the shape and size of the blind hole 21 also meet the requirements of hydrodynamic design, etc. Its test performance fully meets the requirements that when observing the results with the naked eye within 4 minutes in the Rose Bengal plate agglutination test specified in "Diagnostic Techniques for Animal Brucellosis" (GB / T 18646-2018), the agglutination particles are easy to distinguish, and at the same time, it can also meet the requirement that the agglutinated mixture still has fluidity.

[0026] Those skilled in the art should understand that the above embodiments are only used to illustrate rather than limit the present utility model. For example, the plate agglutination test device of the present utility model can also be equally applicable to the plate agglutination test of other animal diseases such as glanders and pullorum disease.

Claims

1. A brucellosis red plate agglutination test device, characterized in that, include: A reaction substrate is integrally molded from a milky white opaque resin, has a reaction front surface and a support back surface, wherein an array of blind holes is formed, each blind hole defines a circular opening on the reaction front surface of the reaction substrate, and defines an inner circumferential wall and a circular bottom along the thickness direction of the reaction substrate, wherein the diameter of the inner circumferential wall of each blind hole gradually decreases from the circular opening to the circular bottom, the diameter of the circular opening is 19 to 21 mm, and the diameter of the circular bottom is 15 to 17 mm; The stirring comb is formed with at least one row of stirring needles parallel to each other, which respectively correspond to at least one row of blind holes in the blind hole array so as to extend therein for stirring.

2. The brucellosis red bengal plate agglutination test device as claimed in claim 1, characterized in that: The blind hole array is composed of a plurality of evenly distributed blind holes, wherein on the reaction front surface of the reaction substrate, a corresponding hole position number is arranged at a set position near the circular opening of each blind hole.

3. The brucellosis red bengal plate agglutination test device as claimed in claim 1, characterized in that: The depth of each blind hole is 1 to 3 mm.

4. The brucellosis red bengal plate agglutination test device as claimed in claim 1, characterized in that: The reaction substrate is made of hard hydrophilic modified ABS resin, and the stirring comb is made of soft polypropylene.

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