Micro-column gel card and sample injection puncture device

Abstract

The utility model relates to the technical field of medical equipment, and particularly discloses a microcolumn gel card and a sample injection puncture device.The microcolumn gel card comprises a fixing plate and a plurality of tubular columns, the tubular columns are all fixed to the fixing plate, a sample injection cavity, a reaction cavity and a gel column cavity are formed in each tubular column, and the sample injection cavity is communicated with the reaction cavity. The gel column cavities are arranged at the lower ends of the tubular columns and are used for loading gel reagents, the sample adding cavities are arranged on the upper end surfaces of the tubular columns, the reaction cavities are communicated with the sample adding cavities and the gel column cavities, the gel column cavities of the tubular columns are arranged in a row along the length direction of the fixing plate, and the reaction cavities are communicated with the gel column cavities of the tubular columns. The sample adding cavities of the plurality of tubular columns are distributed in two rows in a staggered manner, and the center of each row of sample adding cavities deviates from the center line of the fixed plate. As the gel column cavities of the plurality of tubular columns are arranged in a row in the length direction of the fixed plate, the gel column cavities cannot be overlapped in photographing interpretation, interpretation cannot be influenced, and the improvement of the interpretation accuracy is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, and particularly relates to a microcolumn gel card and a sampling puncture device. Background Art

[0002] The microcolumn gel method is a recommended method for international safe blood transfusion inspection. As the core of the microcolumn gel method, the microcolumn gel card is mainly used in blood type tests before surgery and blood transfusion, and screening for hemolytic disease of the newborn during pregnancy and preconception.

[0003] Generally, a microcolumn gel card is formed by parallel connection of multiple microtubes with special shapes. The microtube is provided with a sampling cavity, a reaction pool and a microtube cavity. The microtube cavity contains specific antibodies filled according to the detection requirements of different items and non-dissolvable gel particles with certain physical properties and chemical stability. The added samples and reagents first react in the reaction pool, and then are centrifuged and read by an instrument.

[0004] Currently, the common microcolumn gel cards are 6-column or 8-column. Limited by the number of columns, the detection speed is relatively slow when used with an automated instrument. Therefore, it is urgent to improve the microcolumn gel card to improve the detection efficiency of large-scale experiments without affecting the accuracy of detection results. Summary of the Utility Model

[0005] The utility model aims to solve the technical problem of reading errors existing in the prior art. For this purpose, the utility model provides a microcolumn gel card, which improves the detection efficiency of large-scale experiments by optimizing the position arrangement of the microtubes. At the same time, it reduces the impact on photo reading and improves the reading accuracy.

[0006] The utility model also provides a sampling puncture device applying the microcolumn gel card.

[0007] A microcolumn gel card according to an embodiment of the utility model includes a fixing plate and a plurality of microtubes. The plurality of microtubes are all fixed on the fixing plate. A sampling cavity, a reaction cavity and a gel column cavity are arranged inside the microtube. The gel column cavity is arranged at the lower end of the microtube and is used for loading gel reagents. The sampling cavity is arranged on the upper end surface of the microtube. The reaction cavity communicates with the sampling cavity and the gel column cavity. The gel column cavities of the plurality of microtubes are arranged in a row along the length direction of the fixing plate, and the sampling cavities of the plurality of microtubes are staggered and distributed in two rows. The center of each row of sampling cavities deviates from the central dividing line of the fixing plate.

[0008] The microcolumn gel card according to an embodiment of the utility model has at least the following beneficial effects:

[0009] Since, in the length direction of the fixed plate, the gel column cavities of multiple tube columns are arranged in a row, the gel column cavities will not overlap during photographing and interpretation, which will not affect the interpretation and helps improve the accuracy of interpretation.

[0010] According to some embodiments of the first aspect of the present invention, the vertical projection of the gel column cavity and the sample loading cavity is internally tangent.

[0011] According to some embodiments of the first aspect of the present invention, the reaction cavity is an inclined conical shape, the large end of the reaction cavity is connected to the sample loading cavity, the small end of the reaction cavity is connected to the gel column cavity, and the center distance between two adjacent gel column cavities is less than the diameter of the sample loading cavity.

[0012] According to some embodiments of the first aspect of the present invention, the diameter of the gel column cavity is less than one-half of the diameter of the sample loading cavity.

[0013] According to some embodiments of the first aspect of the present invention, a horizontal panel is provided at the upper end of the fixed plate, the upper end of the tube column extends beyond the panel and forms a flange portion, the flange portion is circular, and a step is provided on the inner wall.

[0014] According to some embodiments of the first aspect of the present invention, vertical reinforcing ribs are provided on the outer wall of the tube column, and the reinforcing ribs extend to the panel.

[0015] According to some embodiments of the first aspect of the present invention, there is a gap between the side surface of the panel and the diameter of the flange portion, and the width of the panel is less than 2 times the outer diameter of the flange portion.

[0016] According to the sample loading puncture device of the second aspect embodiment of the present invention, it includes a machine base and the micro-column gel card of the first aspect embodiment. The machine base is provided with a fixture to fix the micro-column gel card, the machine base is provided with a puncture mechanism, the puncture mechanism is provided with a puncture needle that can be lifted and lowered, and the puncture mechanism can move above the micro-column gel card.

[0017] According to some embodiments of the second aspect of the present invention, the puncture needles correspond to the tube columns one by one, multiple puncture needles are divided into multiple groups, each group of puncture needles is fixed to a sliding seat, and the sliding seat is connected with a driving component.

[0018] According to some embodiments of the second aspect of the present invention, multiple puncture needles are divided into four groups, the puncture mechanism is provided with two driving components, the driving component includes a motor, the output shaft of the motor is connected with a gear, and a sliding seat is arranged on both sides of each gear. The sliding seat is connected with a rack that meshes with the gear.

[0019] According to some embodiments of the second aspect of the present utility model, the fixing plate has two vertical side walls, and a positioning notch is provided on one of the vertical side walls, and the fixture is provided with a positioning boss that cooperates with the positioning notch.

[0020] Additional aspects and advantages of the present utility model will be given in part in the following description, become apparent in part from the following description, or be understood through the practice of the present utility model. Description of the Drawings

[0021] The additional aspects and advantages of the present utility model will become apparent and be easily understood in conjunction with the description of the embodiments with reference to the following drawings, in which:

[0022] Figure 1 is a schematic structural view of a microcolumn gel card according to an embodiment of the first aspect of the present utility model;

[0023] Figure 2 is a front view of a microcolumn gel card according to an embodiment of the first aspect of the present utility model;

[0024] Figure 3 is Figure 2 the A-A cross-sectional view of the microcolumn gel card in

[0025] Figure 4 is a top view of a microcolumn gel card according to an embodiment of the first aspect of the present utility model;

[0026] Figure 5 is a bottom view of a microcolumn gel card according to an embodiment of the first aspect of the present utility model;

[0027] Figure 6 is a cross-sectional view of a microcolumn gel card according to an embodiment of the first aspect of the present utility model;

[0028] Figure 7 is a schematic structural view of a sample adding and puncturing device according to an embodiment of the second aspect of the present utility model;

[0029] Figure 8 is a front view of a puncturing mechanism according to an embodiment of the second aspect of the present utility model;

[0030] Figure 9 is a left view of a puncturing mechanism according to an embodiment of the second aspect of the present utility model;

[0031] Figure 10 is a bottom view of a puncturing mechanism according to an embodiment of the second aspect of the present utility model.

[0032] The reference numerals are as follows:

[0033] Microcolumn gel card 100;

[0034] Fixing plate 200, positioning notch 201, panel 210, reinforcing rib 220;

[0035] Column 300, sample addition chamber 301, reaction chamber 302, gel column chamber 303, flange portion 310;

[0036] Base 400, fixture 410;

[0037] Puncturing mechanism 500, puncturing needle 510, sliding seat 520, motor 530, gear 540, rack 550. Specific embodiments

[0038] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation to the present utility model.

[0039] In the description of the present utility model, it should be understood that the orientation descriptions such as up, down, front, back, left, right, etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0040] In the description of the present utility model, unless otherwise clearly defined, words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in the present utility model in combination with the specific content of the technical solution.

[0041] Referring to Figures 1 to 6 , an embodiment of the first aspect of the present utility model provides a micro-column gel card 100. The micro-column gel card 100 includes a fixing plate 200 and a plurality of columns 300. The plurality of columns 300 are all fixed on the fixing plate 200. The fixing plate 200 is a flat plate member, and its main function is to connect the plurality of columns 300 into one body and determine the positions of the plurality of columns 300. In addition, the fixing plate 200 is also the basis for the micro-column gel card 100 to connect to other devices. In the sample addition and puncturing device, the fixing plate 200 of the micro-column gel card 100 is inserted into the card slot to position the micro-column gel card 100.

[0042] The column 300 is provided with a sample loading chamber 301, a reaction chamber 302 and a gel column chamber 303, wherein the sample loading chamber 301 is used to load samples and / or reagents, the reaction chamber 302 is used for samples and reagents to react, and the gel column chamber 303 is used to load gel reagents, which are pre-filled in the gel column chamber 303. During the experiment, samples and reagents are injected from the sample loading chamber 301 for reaction, and the gel reagents include but are not limited to gel reagents for ABO blood type detection, ABO positive and negative typing and Rh (DCE) blood type detection, ABORhD blood type detection, Rh blood type antigen detection and anti-human protein detection. The sample loading chamber 301 is arranged on the upper end surface of the column 300 to facilitate the injection of samples and / or reagents, the gel column chamber 303 is arranged at the lower end of the column 300, and the reaction chamber 302 connects the sample loading chamber 301 and the gel column chamber 303, and the reaction chamber 302 provides a reaction space so that the injected samples and reagents can contact and react.

[0043] like Figure 4 and Figure 5 As shown, in order to improve the compactness of the structure, reduce the overall occupied space of the microcolumn gel card 100, facilitate use, and improve the detection efficiency of large-scale experiments, the multiple columns 300 are staggered and closely arranged, and in the length direction of the fixed plate 200, the gel column cavities 303 of the multiple columns 300 are arranged in a row, so that two adjacent gel column cavities 303 are separated and have a clear separation area, and the sample loading cavities 301 of the multiple columns 300 are staggered and distributed in two rows, and the center of each row of sample loading cavities 301 deviates from the center line of the fixed plate 200, that is, the two rows of sample loading cavities 301 are distributed on both sides of the center line of the fixed plate 200, and the center line of the fixed plate 200 is the center line in the width direction, that is, the vertical center axis of the sample loading cavity 301 and the vertical center axis of the corresponding gel column cavity 303 are eccentrically staggered, so that when injecting samples / reagents, it is convenient to contact the sample and the reagent with the inner wall of the reaction chamber 302 for sufficient reaction.

[0044] It can be understood that, with the above structure, in the length direction of the fixing plate 200, the gel column cavities 303 of multiple tube columns 300 are arranged in a row. During photographing and interpretation, the gel column cavities 303 do not overlap, which will not affect the interpretation and helps to improve the accuracy of interpretation. Moreover, multiple sample loading cavities 301 are arranged closely and staggeredly. While increasing the detection throughput of the card, the structure of the whole card is compact, occupying a small space, and there is enough space between adjacent sample loading cavities 301, which is beneficial for the sample loading puncture device to puncture and inject samples and / or reagents. It can be understood that during the experiment, when injecting samples and reagents, the samples and reagents first react, and then are centrifuged to contact the gel reagent in the gel column cavity 303. To ensure more accurate experimental results, the samples and reagents can be dropped on the inner wall of the reaction cavity 302 to react first. The inner wall of the reaction cavity 302 is usually conical. If the sample loading cavity 301 and the gel column cavity 303 are concentrically designed, when the operator adjusts the sample loading position, it is necessary to take the center of the sample loading cavity 301 as the reference and then make an appropriate offset so that the sample / reagent can be filled onto the inner wall of the reaction cavity 302. In the embodiment of the present application, the vertical central axis of the sample loading cavity 301 does not coincide with the vertical central axis of the gel column cavity 303, that is, the sample loading cavity 301 of each tube column 300 and its corresponding gel column cavity 303 are eccentrically designed. When the operator adjusts the sample loading position, only the center position of the sample loading cavity 301 (i.e., the vertical central axis of the sample loading cavity) needs to be used as the reference for adjustment, reducing the workload and at the same time improving the consistency of the sample loading positions adjusted by different operators. In some embodiments, the inner diameter (diameter) of the gel column cavity 303 is less than half of the inner diameter (diameter) of the sample loading cavity 301. For example, the inner diameter (diameter) size of the gel column cavity 303 is 3 mm, and the inner diameter (diameter) size of the sample loading cavity 301 is 8 mm. When the operator adjusts the sample loading position and takes the center position of the sample loading cavity 301 as the reference, at this time, in the vertical direction, corresponding to the inner wall of the reaction cavity 302. Therefore, only by taking the center position of the sample loading cavity 301 as the reference to adjust the sample loading position, the sample / reagent can be filled onto the inner wall of the reaction cavity 302 when injecting the liquid; it should be noted that those skilled in the art should understand that the limitation of the relationship between the inner diameter sizes of the gel column cavity 303 and the sample loading cavity 301 in this embodiment is only one implementation case, so that when taking the center position of the sample loading cavity 301 as the reference to adjust the sample loading position and injecting the liquid, the samples and reagents are dropped on the inner wall of the reaction cavity 302 to react fully first, making the experimental results more accurate. And those skilled in the art can also design the inner diameter of the gel column cavity 303 and the inner diameter size of the sample loading cavity 301 into other limited or non-limited size relationships in the case of sacrificing the corresponding degree of result accuracy or sacrificing the convenience of sample loading position adjustment, etc., all of which fall within the protection scope of the present invention.

[0045] Refer to Figure 6, in some embodiments of the first aspect of the present utility model, considering that the cross-sectional area of the gel column cavity 303 is much smaller than that of the sample loading cavity 301, in order to obtain sufficient space to arrange multiple sample loading cavities 301, it is necessary to set the sample loading cavity 301 in a direction deviating from the central dividing line of the fixing plate 200. The gel column cavity 303 and the sample loading cavity 301 are necessarily arranged non-concentrically. In order to facilitate the injection of the gel reagent into the gel column cavity 303, the gel column cavity 303 and the sample loading cavity 301 are set to be internally tangent in the vertical projection. When using an injection needle to inject the gel reagent, the injection needle can directly extend into the gel column cavity 303, and the gel reagent can not contact the inner walls of the sample loading cavity 301 and the reaction cavity 302. As Figure 3 shown, since the tube column 300 has a certain wall thickness, the outer contour line at the sample loading cavity 301 can be set to be tangent or intersecting, that is, two adjacent tube columns 300 share the wall thickness at the sample loading cavity 301, as long as it can be ensured that the two sample loading cavities 301 are separated, which helps to closely arrange multiple tube columns 300 and reduce the space occupation.

[0046] Referring to Figure 1 and Figure 6 , in some embodiments of the first aspect of the present utility model, since the gel column cavity 303 and the sample loading cavity 301 are eccentrically arranged, in order to connect the gel column cavity 303 and the sample loading cavity 301, the reaction cavity 302 is set to be an oblique cone shape, wherein the large end of the reaction cavity 302 is connected to the sample loading cavity 301, and the small end of the reaction cavity 302 is connected to the gel column cavity 303, achieving a smooth transition in structure. Moreover, the oblique cone-shaped reaction cavity 302 is beneficial to the injection of samples and the flow of reagents. And the center distance between two adjacent gel column cavities 303 is less than the diameter of the sample loading cavity 301, so that the vertical projections of two adjacent tube columns 300 partially overlap, improving the tightness of the arrangement and reducing the space occupation.

[0047] It can be understood that during the use of the micro-column gel card 100, first inject the gel reagent into the gel column cavity 303, and then cover the sample loading cavity 301 with a film. When it is necessary to inject samples and / or reagents, pierce the sealing film. In order to facilitate the film covering operation, referring to Figure 1 , in some embodiments of the first aspect of the present utility model, a horizontal panel 210 is provided at the upper end of the fixing plate 200. The upper end of the tube column 300 extends beyond the panel 210 and forms a flange portion 310. The flange portion 310 is circular, and the sealing film covers the flange portion 310, facilitating the sealing film pressing process. A step can also be provided on the inner wall of the flange portion 310 to press the sealing film through the step, promoting the close fit of the sealing film and the flange portion 310, ensuring reliable sealing.

[0048] Referring to Figure 1, in some embodiments of the first aspect of the present utility model, vertical reinforcing ribs 220 are provided on the outer wall of the pipe column 300, and the reinforcing ribs 220 extend to the panel 210, strengthening the connection structure stability between the pipe column 300 and the panel 210, increasing the structural strength and stiffness of the pipe column 300, and reducing the risk of deformation of the pipe column 300. Moreover, the structures of the multiple reinforcing ribs 220 are the same, and the multiple reinforcing ribs 220 can be used to place the micro-column gel card 100 horizontally, facilitating stacking storage and use in a horizontal state.

[0049] Refer to Figure 2 , in some embodiments of the first aspect of the present utility model, there is a gap between the side surface of the panel 210 and the flange portion 310, that is, the vertical projection of the pipe column 300 entirely falls within the panel 210, and the width of the panel 210 is less than twice the outer diameter of the flange portion 310, such that the vertical projections of two adjacent pipe columns 300 partially overlap, improving the tightness of the arrangement and reducing space occupation.

[0050] Refer to Figures 7 to 10 , embodiments of the second aspect of the present utility model propose a sample addition and puncture device. The sample addition and puncture device includes a machine base 400 and the micro-column gel card 100 of the embodiments of the first aspect. The machine base 400 is provided with a fixture 410, and the fixture 410 clamps and fixes the fixing plate 200 of the micro-column gel card 100 to fix the micro-column gel card 100. Moreover, a puncture mechanism 500 is provided on the machine base 400, and the puncture mechanism 500 is provided with a puncture needle 510 that can be lifted and lowered. The puncture mechanism 500 can move above the micro-column gel card 100 such that the puncture needle 510 is located above the sample addition cavity 301. The puncture needle 510 can be used to pierce the film on the micro-column gel card 100, and then inject a sample and / or reagent into the sample addition cavity 301 to carry out a reaction.

[0051] The micro-column gel card 100 includes a fixing plate 200 and multiple pipe columns 300. In the length direction of the fixing plate 200, the gel column cavities 303 of the multiple pipe columns 300 are arranged in a row, such that two adjacent gel column cavities 303 are separated, having a clear separation area. The sample addition cavities 301 of the multiple pipe columns 300 are staggered and distributed in two rows, and the center of each row of sample addition cavities 301 deviates from the central dividing line of the fixing plate 200. In photographing and interpretation, the gel column cavities 303 do not overlap, which does not affect the interpretation and helps to improve the accuracy of the interpretation. The sample addition and puncture device includes all the technical solutions of the micro-column gel card 100 and has all the technical effects of the micro-column gel card 100, which will not be elaborated herein.

[0052] It can be understood that, in order to accurately position the micro-column gel card 100, a positioning structure can be provided on the fixing plate 200, such as Figure 1 and Figure 2As shown, the fixing plate 200 has two vertical side walls. A positioning notch 201 can be provided on one of the vertical side walls. The fixture 410 is provided with a positioning boss, and the positioning boss cooperates with the positioning notch 201 to accurately position the micro-column gel card 100 and prevent incorrect installation. At the same time, it also has an anti-fooling function. After each micro-column gel card 100 is filled with glue and covered with a film, it needs to be boxed. During the boxing process, the positioning notch 201 can also be used to prevent incorrect placement. In addition, the positioning structure can also be selected as other structures such as buckles and positioning pins, as long as it can achieve the positioning of the micro-column gel card 100.

[0053] Referring Figure 10 , in some embodiments of the second aspect of the present invention, the puncture needles 510 correspond to the tube columns 300 one by one, and the positions of the multiple puncture needles 510 and the multiple tube columns 300 also correspond one by one. The multiple puncture needles 510 can be divided into multiple groups, for example, divided into four groups. Each group of puncture needles 510 is fixed to a sliding seat 520, and the sliding seat 520 is connected to a driving component, and the driving component drives the sliding seat 520 and the puncture needles 510 to move up and down, so as to pierce the film on the micro-column gel card 100.

[0054] It can be understood that the driving component can adopt various structures, such as Figure 8 and Figure 9 As shown, twelve puncture needles 510 are used. The twelve puncture needles 510 are divided into four groups (for example, each group of three puncture needles 510 corresponds to three tube columns 300 for one-person experiment during the detection process). The puncture mechanism is provided with two driving components. The driving component includes a motor 530, and the output shaft of the motor 530 is connected to a gear 540. A sliding seat 520 is arranged on both sides of each gear 540, and the sliding seat 520 is connected to a rack 550 that meshes with the gear 540. The motor 530 drives the gear 540 to rotate. Through the cooperation of the gear 540 and the rack 550, the sliding seat 520 can be driven to move up and down, so as to realize the up and down movement of the puncture needles 510 to pierce the film on the micro-column gel card 100. The motor 530 can continuously drive the sliding seat 520 and the puncture needles 510 to move up and down through forward and reverse cyclic rotation, improving the efficiency of piercing the film and reducing the time used, and improving the production efficiency. The four groups of puncture needles 510 perform punctures crosswise, avoiding needle insertion at the same time, and adjacent groups will not interfere. It should be understood that the puncture mechanism can also be provided with one driving component, three driving components or more driving components. The puncture needles can be lifted and lowered together to pierce the film on the micro-column gel card 100 synchronously, or can be lifted and lowered crosswise to pierce the film on the micro-column gel card 100 crosswise, which can be determined according to actual usage requirements, and all fall within the protection scope of the present invention.

[0055] In addition, the driving component can also be a motor-driven synchronous belt. A sliding seat 520 is arranged on each side of the axis of the motor. The sliding seat 520 is fixed on the synchronous belt. The motor 530 can continuously drive the sliding seat 520 and the puncture needle 510 to move up and down through forward and reverse cyclic rotation. The driving component can also be a motor-driven cam rotation, using the cam to drive the sliding seat 520 and the puncture needle 510 to move up and down. Other structures of the driving component will not be elaborated one by one. As long as it can realize the up and down movement of the sliding seat 520 and the puncture needle 510, it is okay.

[0056] The embodiments of the present invention have been described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can also be made without departing from the gist of the present invention.

Claims

1. A microcolumn gel card, characterized in that: include: Fixed plate; A plurality of tube columns are fixed to the fixed plate, wherein a sample loading chamber, a reaction chamber and a gel column chamber are arranged inside the tube columns, wherein the gel column chamber is arranged at the lower end of the tube column and is used to load a gel reagent, wherein the sample loading chamber is arranged at the upper end surface of the tube column, and the reaction chamber communicates with the sample loading chamber and the gel column chamber, wherein the gel column chambers of the plurality of tube columns are arranged in a row along the length direction of the fixed plate, and the sample loading chambers of the plurality of tube columns are staggeredly distributed in two rows, wherein the center of each row of the sample loading chambers deviates from the center dividing line of the fixed plate.

2. The microcolumn gel card according to claim 1, characterized in that: The vertical projections of the gel column cavity and the sample adding cavity are inwardly tangent.

3. The microcolumn gel card according to claim 2, characterized in that: The reaction chamber is in an oblique cone shape, the large end of the reaction chamber is connected to the sample adding chamber, the small end of the reaction chamber is connected to the gel column chamber, and the center distance between two adjacent gel column chambers is smaller than the diameter of the sample adding chamber.

4. The microcolumn gel card according to claim 1, characterized in that: The inner diameter of the gel column cavity is less than half of the inner diameter of the sample adding cavity.

5. The microcolumn gel card according to claim 1, characterized in that: A horizontal panel is arranged at the upper end of the fixing plate, and the upper end of the pipe column exceeds the panel to form a flange portion, and the flange portion is in a circular ring shape.

6. The microcolumn gel card according to claim 5, characterized in that: The outer wall of the pipe column is provided with vertically arranged reinforcing ribs, and the reinforcing ribs extend to the panel.

7. The microcolumn gel card according to claim 5, characterized in that: There is a gap between the side surface of the panel and the diameter of the flange portion, and the width of the panel is less than twice the outer diameter of the flange portion.

8. A sample adding and puncturing device, characterized in that: It comprises a base and a microcolumn gel card as described in any one of claims 1 to 7, wherein the base is provided with a clamp to fix the microcolumn gel card, the base is provided with a puncture mechanism, the puncture mechanism is provided with a puncture needle that can be raised and lowered, and the puncture mechanism can be moved above the microcolumn gel card.

9. The sample addition and puncturing device according to claim 8, characterized in that: The puncture needles correspond to the tube columns one by one, and the plurality of puncture needles are divided into a plurality of groups. Each group of puncture needles is fixed to a slide seat, and the slide seat is connected to a driving assembly.

10. The sample addition and puncturing device according to claim 9, characterized in that: The plurality of puncture needles are divided into four groups. The puncture mechanism is provided with two driving assemblies. The driving assembly includes a motor. The output shaft of the motor is connected to a gear. A slide seat is arranged on both sides of each gear. The slide seat is connected to a rack meshing with the gear.

11. The sample addition and puncturing device according to claim 8, characterized in that: The fixing plate has two vertical side walls, one of which is provided with a positioning notch, and the clamp is provided with a positioning boss that matches the positioning notch.

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