Large-scale culture device for plant calluses
By designing a large-scale plant callus culture device including a trumpet, a shunt tube and a fluid separation structure, the problem of artificial collection of callus fluid in batches caused by long-term callus fluid caused by callus death, and efficient and rapid callus fluid collection was achieved.
Patent Information
- Application Number
- CN202422271938.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-18
- Publication Date
- 2025-06-27
- Estimated Expiration
- 2034-09-18
AI Technical Summary
Manually collecting callus fluid from sea fennel in batches takes a lot of time, resulting in callus prone to death.
A large-scale cultivation device for plant callus tissue was designed, including a base plate, support rod, trumpet, shunt tube, culture tube and liquid separation structure. Through the design of the liquid separation structure, multiple parts of callus fluid can be collected at the same time, and the collection amount can be changed and the manual confirmation time can be reduced through the adjustment of the threaded cylinder. The arrangement of the horn and shunt tube allows the excess callus fluid to flow directly back to the culture tube to avoid back and forth transfer to cause death.
It improves batch collection efficiency, reduces the risk of death of callus in sea anise, saves a lot of artificial time, and achieves faster and more efficient callus fluid collection.
Smart Images
Figure CN223025155U_ABST
Abstract
Description
Technical Field
[0001] The utility model mainly relates to the technical field of large-scale culture of plant callus, and specifically relates to a device for large-scale culture of plant callus. Background Art
[0002] Crithmum maritimum is a plant with unique self-healing ability and has good medical effects on skin sunburn. It is widely used in cosmetics. Due to the difficult growth of Crithmum maritimum, Crithmum maritimum is extremely scarce.
[0003] In order to avoid damage to the ecology, the method of artificially culturing Crithmum maritimum on a large scale is adopted to obtain Crithmum maritimum callus. When culturing artificially, it is necessary to batch collect or reculture the cultured Crithmum maritimum callus liquid. Manual batching requires a lot of time, resulting in the easy death of Crithmum maritimum callus during the batching process. Therefore, a new device for large-scale culture of plant callus is needed. Summary of the Utility Model
[0004] The technical solution of the utility model provides a solution significantly different from the prior art in view of the prior art solution. It mainly provides a device for large-scale culture of plant callus to solve the technical problem that manual batching of Crithmum maritimum callus liquid requires a lot of time, resulting in the easy death of Crithmum maritimum callus as mentioned in the above background art.
[0005] The technical solution adopted by the utility model to solve the above technical problems is as follows:
[0006] A device for large-scale culture of plant callus includes a bottom plate. A plurality of support rods inclined towards the center of the bottom plate are installed on the upper surface of the bottom plate. A horn-shaped cylinder is installed at one end of the plurality of support rods away from the bottom plate. A culture cylinder is installed at the bottom of the horn-shaped cylinder. An extrusion member is slidably connected in the culture cylinder. A thread is provided on the circumferential surface of the culture cylinder. A liquid distribution member is threadedly connected to the circumferential surface of the culture cylinder. A plurality of shunt pipes are installed on the circumferential surface of the horn-shaped cylinder. The liquid distribution member is sleeved on the circumferential surface of the shunt pipes.
[0007] Preferably, the extrusion member includes a piston. The piston is slidably connected in the culture cylinder. A bearing is installed on the lower surface of the piston. A screw rod is installed in the bearing. A circular plate is installed at the bottom of the culture cylinder. A threaded hole is provided on the upper surface of the circular plate. The screw rod is threadedly connected in the threaded hole.
[0008] Preferably, a knob is installed at one end of the screw rod away from the bearing.
[0009] Preferably, the liquid separation member includes a threaded cylinder, which is threadedly connected to the annular surface of the culture cylinder. An annular groove is provided on the annular surface of the threaded cylinder, and a circular ring is rotatably connected in the annular groove. A plurality of round holes are provided on the upper surface of the circular ring, and a collection tube is inserted into the round holes. The end of the collection tube away from the round hole is sleeved on the annular surface of the shunt tube.
[0010] Preferably, a conical cylinder is installed on the upper surface of the collection tube.
[0011] Preferably, a sealing ring is installed on the annular surface of the shunt tube, and the collection tube is in sliding contact with the sealing ring.
[0012] Preferably, a sealing cover is installed on the upper surface of the horn-shaped cylinder.
[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0014] 1. By threadedly connecting a liquid separation structure composed of a threaded cylinder, a circular ring and a collection tube to the annular surface of the culture cylinder, the liquid separation structure is used to collect multiple portions of Crithmum maritimum callus liquid at the same time, improving the batch collection efficiency.
[0015] 2. By adjusting the position of the threaded cylinder on the annular surface of the culture cylinder, the length of the shunt tube inserted into the collection tube is changed, and thus the collection amount is changed, saving the time required for manual collection to confirm the collection amount in turn, and further accelerating the collection efficiency.
[0016] 3. By installing the horn-shaped cylinder on the upper surface of the culture cylinder and then arranging the shunt tube on the annular surface of the horn-shaped cylinder, the excess Crithmum maritimum callus liquid during the batch collection process directly flows back into the culture cylinder, avoiding the death of Crithmum maritimum callus caused by back-and-forth transfer.
[0017] The following will combine the drawings with specific embodiments to explain the present utility model in detail. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 is a three-dimensional schematic diagram of the present utility model;
[0019] Figure 2 is a cross-sectional view of the present utility model;
[0020] Figure 3 is the Figure 2 enlarged schematic view of part A in the present utility model.
[0021] Markings in the figure:
[0022] 1. Bottom plate; 2. Support rod; 3. Sealing cover; 4. Horn-shaped cylinder; 5. Diverging pipe; 6. Conical cylinder; 7. Collection pipe; 8. Ring; 9. Threaded cylinder; 10. Knob; 11. Screw rod; 12. Bearing; 13. Piston; 14. Circular plate; 15. Culture cylinder; 16. Sealing ring. Detailed implementation manner
[0023] To facilitate the understanding of the present utility model, the present utility model will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present utility model are given in the drawings. However, the present utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the present utility model more thorough and comprehensive.
[0024] It should be noted that when an element is referred to as being "fixedly installed on" another element, it can be directly on the other element or there can be an intermediate element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are only for the purpose of illustration.
[0025] Unless otherwise defined, all technical and scientific terms used in this article have the same meaning as commonly understood by those skilled in the technical field to which the present utility model belongs. The terms used in the description of the present utility model in this article are only for the purpose of describing specific embodiments and are not intended to limit the present utility model. The term "and / or" used in this article includes any and all combinations of one or more of the related listed items.
[0026] Please refer specifically to the attached Figures 1 - 3 A large-scale culture device for plant callus, comprising a bottom plate 1, on the upper surface of the bottom plate 1, a plurality of support rods 2 are installed and arranged obliquely towards the center of the bottom plate 1. At the ends of the plurality of support rods 2 away from the bottom plate 1, a horn-shaped cylinder 4 is installed. A plurality of diverging pipes 5 are installed on the circumferential surface of the horn-shaped cylinder 4. At the bottom of the horn-shaped cylinder 4, a culture cylinder 15 is installed. A piston 13 is slidably connected in the culture cylinder 15. A bearing 12 is installed on the lower surface of the piston 13. A screw rod 11 is installed in the bearing 12. Then, a circular plate 14 is installed at the bottom of the culture cylinder 15. A threaded hole is opened on the upper surface of the circular plate 14. The screw rod 11 is threadedly connected in the threaded hole.
[0027] The height of the piston 13 in the culture cylinder 15 is adjusted by using the screw rod 11, so as to raise the liquid level in the culture cylinder 15, so that the callus liquid of Crithmum maritimum in the culture cylinder 15 flows out from the diverging pipe 5. And in order to facilitate the rotation of the screw rod 11, a knob 10 is installed at the end of the screw rod 11 away from the bearing 12.
[0028] By installing the horn 4 on the upper surface of the culture cylinder 15 and then arranging the shunt pipe 5 on the annular surface of the horn 4, the excess Crithmum maritimum callus liquid during the batch collection process can directly flow back into the culture cylinder 15, avoiding the death of Crithmum maritimum callus caused by back-and-forth transfer.
[0029] Threads are provided on the annular surface of the culture cylinder 15, and a threaded cylinder 9 is threadedly connected at the threaded part. An annular groove is opened on the annular surface of the threaded cylinder 9, and a ring 8 is rotatably connected in the annular groove. A plurality of round holes are opened on the upper surface of the ring 8, and a collection pipe 7 is inserted into the round holes. The end of the collection pipe 7 away from the round hole is sleeved on the annular surface of the shunt pipe 5.
[0030] By threadedly connecting the liquid separation structure composed of the threaded cylinder 9, the ring 8 and the collection pipe 7 on the annular surface of the culture cylinder 15, the liquid separation structure is used to collect multiple portions of Crithmum maritimum callus liquid at the same time, improving the batch collection efficiency. Then, the position of the threaded cylinder 9 on the annular surface of the culture cylinder 15 is adjusted, so that the length of the shunt pipe 5 inserted into the collection pipe 7 changes, and further the collection amount changes, saving the time required for manual collection to confirm the collection amount in turn, and further accelerating the collection efficiency.
[0031] When the Crithmum maritimum callus liquid flows out of the shunt pipe 5, it will fall into the collection pipe 7, thus completing the collection of the Crithmum maritimum callus liquid.
[0032] In order to facilitate the insertion of the collection pipe 7 on the annular surface of the collection pipe 7, a conical cylinder 6 is installed on the upper surface of the collection pipe 7.
[0033] In order to ensure the sealing performance between the shunt pipe 5 and the collection pipe 7, a sealing ring 16 is installed on the annular surface of the shunt pipe 5, and the collection pipe 7 is in sliding contact with the sealing ring 16.
[0034] In order to prevent dust from entering the culture cylinder 15, a sealing cover 3 is installed on the upper surface of the horn 4. Since a plurality of shunt pipes 5 are installed on the annular surface of the horn 4, the sealing of the horn 4 by the sealing cover 3 will not affect the air flow in the culture cylinder 15.
[0035] The specific operation process of this utility model is as follows: By rotating the screw 11, the position of the piston 13 in the culture cylinder 15 is raised, and then more Crithmum maritimum callus liquid is squeezed to rise in the culture cylinder 15 until it reaches the shunt pipe 5.
[0036] The height is adjusted through the threaded fit between the threaded cylinder 9 and the annular surface of the culture cylinder 15, so that the heights of the plurality of collection pipes 7 on the ring 8 are adjusted. Then, by rotating the ring 8, the collection pipes 7 are accurately inserted into the shunt pipe 5, so as to collect the Crithmum maritimum callus liquid entering the shunt pipe 5.
[0037] As the liquid level in the culture cylinder 15 rises until the liquid submerges the shunt pipe 5, at this time, the required amount of the Crithmum maritimum callus liquid collected by the collecting pipe 7 is reached. Subsequently, the screw 11 is rotated in one direction to drive the piston 13 to descend in height, so that the liquid level in the culture cylinder 15 is reduced again. Subsequently, the threaded cylinder 9 is removed, and then multiple collecting pipes 7 filled with the Crithmum maritimum callus liquid can be taken out, thereby achieving the collection of a large amount of the Crithmum maritimum callus liquid at the same time.
[0038] The above has made an exemplary description of the present invention in conjunction with the drawings. Obviously, the specific implementation of the present invention is not limited by the above methods. As long as such non-substantial improvements are made by adopting the method concept and technical solution of the present invention, or the concept and technical solution of the present invention are directly applied to other occasions without improvement, they are all within the protection scope of the present invention.
Claims
1. A plant callus scale culture device, comprising a bottom plate (1), characterized in that: The upper surface of the base plate (1) is provided with a plurality of support rods (2) arranged obliquely toward the center of the base plate (1); a horn (4) is provided at one end of the plurality of support rods (2) away from the base plate (1); a culture cylinder (15) is provided at the bottom of the horn (4); an extrusion piece is slidably connected inside the culture cylinder (15); a thread is provided on the annular surface of the culture cylinder (15); a liquid separation piece is threadedly connected to the annular surface of the culture cylinder (15); a plurality of shunt pipes (5) are provided on the annular surface of the horn (4); and the liquid separation piece is sleeved on the annular surface of the shunt pipe (5).
2. The large-scale culture device for plant callus according to claim 1, characterized in that: The extrusion member comprises a piston (13), the piston (13) is slidably connected in a culture cylinder (15), a bearing (12) is installed on the lower surface of the piston (13), a screw (11) is installed in the bearing (12), a circular plate (14) is installed at the bottom of the culture cylinder (15), a threaded hole is opened on the upper surface of the circular plate (14), and the screw (11) is threadedly connected in the threaded hole.
3. The large-scale culture device for plant callus according to claim 2, characterized in that: A knob (10) is mounted on one end of the screw rod (11) away from the bearing (12).
4. The large-scale culture device for plant callus according to claim 1, characterized in that: The liquid separation component comprises a threaded cylinder (9), wherein the threaded cylinder (9) is threadedly connected to the annular surface of the culture cylinder (15), an annular groove is provided on the annular surface of the threaded cylinder (9), a circular ring (8) is rotatably connected in the annular groove, a plurality of circular holes are provided on the upper surface of the circular ring (8), a collecting tube (7) is inserted in the circular holes, and an end of the collecting tube (7) away from the circular holes is sleeved on the annular surface of the shunt tube (5).
5. The large-scale culture device for plant callus according to claim 4, characterized in that: A conical cylinder (6) is mounted on the upper surface of the collecting tube (7).
6. The large-scale culture device for plant callus according to claim 4, characterized in that: A sealing ring (16) is installed on the annular surface of the flow distribution pipe (5), and the collecting pipe (7) is in sliding contact with the sealing ring (16).
7. The large-scale culture device for plant callus according to claim 1, characterized in that: A sealing cover (3) is installed on the upper surface of the horn (4).