Multistage ultrafiltration equipment

Abstract

The utility model discloses multi-stage ultrafiltration equipment, which belongs to the technical field of filtering equipment, and comprises an equipment main body for separating and purifying nucleic acid, a combined mechanism for assisting in filtering and purifying the nucleic acid is arranged on the equipment main body, the multi-stage ultrafiltration equipment further comprises a multi-stage filtering assembly for performing multi-stage filtering on the nucleic acid, the combination mechanism comprises a moving assembly for feeding and discharging the multi-stage filter assemblies and a pressing assembly for combining the multi-stage filter assemblies, and the moving assembly and the pressing assembly are both connected with the equipment main body. By adopting the mode, the multi-stage filtration and purification of nucleic acid are realized by modifying the structure of the multi-stage filtration assembly, and the feeding and discharging of the multi-stage filtration assembly are facilitated through the moving assembly and the pressing assembly, so that the working efficiency of filtration and purification of nucleic acid is effectively improved.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering equipment, in particular to a multi-stage ultrafiltration equipment. Background Art

[0002] Ultrafiltration technology is a membrane separation technology that can purify and separate solutions. Ultrafiltration tubes are devices used to separate different molecules in liquids, and they utilize the characteristics of ultrafiltration membranes to achieve the separation and concentration of molecules. These centrifuge tubes are commonly used in fields such as biology, biochemistry, molecular biology, pharmaceuticals, and bioengineering to separate and concentrate biomolecules, proteins, nucleic acids, etc.

[0003] For example, Chinese Patent CN213643745U discloses an ultrafiltration tube for nucleic acid research and detection, which includes a centrifuge tube, a filter tube embedded inside the centrifuge tube, and a sealing cover that seals and covers the upper parts of the centrifuge tube and the filter tube. A conical tube is detachably connected to the lower part of the centrifuge tube. Fixed rods are fixedly connected to the outer walls of the centrifuge tube and the conical tube. A screw rod is threadedly inserted inside the fixed rod, and a nut is threadedly sleeved on the lower part of the screw rod. A support rod is fixedly connected to the upper end of the inner wall of the centrifuge tube, and the upper end of the filter tube is placed on the upper part of the support rod.

[0004] However, its technology has the following problems. This technology directly performs ultrafiltration separation and purification on nucleic acids without treating the nucleic acids before separation and purification. Therefore, macromolecular substances in the nucleic acids will adsorb on the filter tube, blocking the orifice of the filter tube and reducing the effect of nucleic acid separation and purification.

[0005] Based on this, the utility model designs a multi-stage ultrafiltration equipment to solve the above problems. Summary of the Utility Model

[0006] In view of the above-mentioned drawbacks of the prior art, the utility model provides a multi-stage ultrafiltration equipment.

[0007] To achieve the above purposes, the utility model is realized through the following technical solutions:

[0008] A multi-stage ultrafiltration equipment, including a device main body for separating and purifying nucleic acids, and a combination mechanism for assisting nucleic acid filtration and purification is installed on the device main body;

[0009] It further includes a multi-stage filtration component for performing multi-stage filtration on nucleic acids;

[0010] The combination mechanism includes a moving component for loading and unloading the multi-stage filtration component and a pressing component for combining the multi-stage filtration component. The moving component and the pressing component are both connected to the device main body.

[0011] Furthermore, the equipment body includes a support platform, a centrifuge, a fixed cover, a loading hole and a working hole. The middle part of the support platform is fixedly connected to the fixed cover, the centrifuge is located at the lower end of the fixed cover, and the side wall of the centrifuge is rotatably connected to the fixed cover. The fixed cover is provided with a loading hole and a working hole.

[0012] Furthermore, the support platform is connected to the moving component.

[0013] Furthermore, the moving assembly includes a first servo motor, a connecting rod, a support plate, a fixed plate, a placement rack, a vortex spring and a rebound plate. The first servo motor is fixedly installed on the lower end of the left side of the support platform, the connecting rod is fixedly installed on the output end of the first servo motor, the upper end of the connecting rod is fixedly connected to the left side of the support plate, the fixed plate is rotatably connected to the lower end side wall of the connecting rod, the fixed plate and the support platform are fixed by bolts, the placement rack is fixedly installed on the right side of the fixed plate, one end of the vortex spring is fixedly connected to the inner end of the placement rack, the other end of the vortex spring is fixedly connected to one end of the rebound plate, and the rebound plate is connected to the multi-stage filter assembly.

[0014] Furthermore, a plurality of vortex springs and rebound plates are arranged at equal intervals.

[0015] Furthermore, the multiple vortex springs and rebound plates are arranged in a circle.

[0016] Furthermore, the support plate is connected to the pressing assembly.

[0017] Furthermore, the pressing assembly includes an electric cylinder, a pulley assembly, a second servo motor, a spline shaft, a spline sleeve, a pressure plate and a suction cup. The electric cylinder and the second servo motor are fixedly mounted on the upper end of the support plate, and the electric cylinder is located on the right side of the second servo motor. The output end of the electric cylinder is rotatably connected to the spline shaft, the spline sleeve is rotatably connected to the lower end of the support plate, the spline shaft is slidably connected to the spline sleeve, the second servo motor is transmission-connected to the spline sleeve through the pulley assembly, the lower end of the spline shaft is fixedly connected to the pressure plate, the lower end of the pressure plate is fixedly connected to the suction cup, the suction cup is located directly above the placement rack, and the suction cup is connected to the multi-stage filter assembly.

[0018] Furthermore, the multi-stage filtration assembly includes an ultrafiltration tube, a slot, a filter paper mounting rack, a threaded groove, an upper cover and a slot. A slot is provided inside the ultrafiltration tube, the lower end of the filter paper mounting rack is in interference contact with the slot, a threaded groove is provided at the upper end of the filter paper mounting rack, the threaded groove is connected to the internal thread of the upper cover, the suction end of the suction cup is suction-connected to the upper end of the upper cover, and a plurality of slots are provided at equal intervals at the lower end of the filter paper mounting rack, and the plurality of slots are respectively connected to a plurality of rebound plates.

[0019] Compared with the prior art, the utility model has the following beneficial effects:

[0020] When the utility model is in use, the multi-stage filtration component is placed in the equipment main body, and the moving component drives the pressing component to load and combine the multi-stage filtration component. Then, the multi-stage filtration component filters nucleic acid in multiple stages. After that, the equipment main body centrifugally purifies the multi-stage filtration component, and then the moving component drives the pressing component to unload the multi-stage filtration component.

[0021] The utility model realizes multi-stage filtration and purification of nucleic acid by transforming the structure of the multi-stage filtration component, and facilitates the loading and unloading of the multi-stage filtration component through the moving component and the pressing component, effectively improving the working efficiency of nucleic acid filtration and purification. Brief Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0023] Figure 1 is a three-dimensional view of a multi-stage ultrafiltration device of the present utility model Figure 1 ;

[0024] Figure 2 is a front view of a multi-stage ultrafiltration device of the present utility model;

[0025] Figure 3 is a left view of a multi-stage ultrafiltration device of the present utility model;

[0026] Figure 4 is a three-dimensional view of a multi-stage ultrafiltration device of the present utility model Figure 2 ;

[0027] Figure 5 is a three-dimensional view of the multi-stage filtration component of the present utility model;

[0028] Figure 6 is along Figure 3 a sectional view taken along the A-A direction;

[0029] Figure 7 is Figure 6 an enlarged view of part B in

[0030] The reference numerals in the drawings respectively represent:

[0031] 1. Equipment main body 11. Support platform 12. Centrifuge 13. Fixed cover 14. Feeding hole position 15. Working hole position 2. Combination mechanism 21. Moving component 211. First servo motor 212. Connecting rod 213. Support plate 214. Fixed plate 215. Placing rack 216. Torsion spring 217. Rebound plate 22. Pressing-down component 221. Electric cylinder 222. Pulley assembly 223. Second servo motor 224. Spline shaft 225. Spline shaft sleeve 226. Pressing plate 227. Suction cup 3. Multi-stage filtration component 31. Ultrafiltration tube 32. Slot 33. Filter paper placement rack 34. Threaded groove 35. Upper cover 36. Card slot. Detailed implementation manners

[0032] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Apparently, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without any creative efforts shall fall within the protection scope of the present utility model.

[0033] The "left", "right", "front", "rear", "upper" and "lower" mentioned in the following description are oriented in the perspective direction of the front view.

[0034] In some embodiments, please refer to the accompanying specification Figures 1-7 , a multi-stage ultrafiltration device, including an equipment main body 1 for separating and purifying nucleic acids, and a combination mechanism 2 for assisting in nucleic acid filtration and purification is installed on the equipment main body 1;

[0035] It further includes a multi-stage filtration component 3 for performing multi-stage filtration on nucleic acids;

[0036] The combination mechanism 2 includes a moving component 21 for loading and unloading the multi-stage filtration component 3 and a pressing-down component 22 for combining the multi-stage filtration component 3, and the moving component 21 and the pressing-down component 22 are both connected to the equipment main body 1.

[0037] When the present utility model is in use, the multi-stage filtration component 3 is placed in the equipment main body 1, the moving component 21 drives the pressing-down component 22 to perform feeding and combination on the multi-stage filtration component 3, then the multi-stage filtration component 3 performs multi-stage filtration on nucleic acids, and then the equipment main body 1 performs centrifugal purification on the multi-stage filtration component 3, and then the moving component 21 drives the pressing-down component 22 to unload the multi-stage filtration component 3;

[0038] This utility model realizes the multi-stage filtration and purification of nucleic acids by transforming the structure of the multi-stage filtration component 3, and facilitates the loading and unloading of the multi-stage filtration component 3 through the moving component 21 and the pressing-down component 22, effectively improving the work efficiency of nucleic acid filtration and purification.

[0039] In some embodiments, as Figures 1-7 shown, as a preferred embodiment of the present utility model, the equipment main body 1 includes a support platform 11, a centrifuge 12, a fixed cover 13, a feeding hole position 14 and a working hole position 15. The middle part of the support platform 11 is fixedly connected to the fixed cover 13. The centrifuge 12 is located at the lower end of the fixed cover 13, and the side wall of the centrifuge 12 is rotationally connected to the fixed cover 13. The fixed cover 13 is provided with a feeding hole position 14 and a working hole position 15.

[0040] The support platform 11 is connected to the moving component 21.

[0041] The moving component 21 includes a first servo motor 211, a connecting rod 212, a support plate 213, a fixing plate 214, a placement rack 215, a scroll spring 216 and a rebound plate 217. The first servo motor 211 is fixedly installed at the lower left end of the support platform 11. The connecting rod 212 is fixedly installed on the output end of the first servo motor 211. The upper end of the connecting rod 212 is fixedly connected to the left side of the support plate 213. The fixing plate 214 is rotationally connected to the side wall of the lower end of the connecting rod 212. The fixing plate 214 is fixedly connected to the support platform 11 by bolts. The placement rack 215 is fixedly installed on the right side of the fixing plate 214. One end of the scroll spring 216 is fixedly connected to the inner end of the placement rack 215. The other end of the scroll spring 216 is fixedly connected to one end of the rebound plate 217. The rebound plate 217 is connected to the multi-stage filtration component 3.

[0042] A plurality of the scroll springs 216 and the rebound plates 217 are provided at equal intervals.

[0043] The plurality of scroll springs 216 and the rebound plates 217 are all arranged in a circular pattern.

[0044] The support plate 213 is connected to the pressing-down component 22.

[0045] The pressing component 22 includes an electric cylinder 221, a pulley assembly 222, a second servo motor 223, a spline shaft 224, a spline shaft sleeve 225, a pressing plate 226, and a suction cup 227. The electric cylinder 221 and the second servo motor 223 are both fixedly installed at the upper end of the support plate 213, and the electric cylinder 221 is located on the right side of the second servo motor 223. The output end of the electric cylinder 221 is rotatably connected to the spline shaft 224. The spline shaft sleeve 225 is rotatably connected to the lower end of the support plate 213. The spline shaft 224 is slidably connected to the spline shaft sleeve 225. The second servo motor 223 is drivingly connected to the spline shaft sleeve 225 through the pulley assembly 222. The lower end of the spline shaft 224 is fixedly connected to the pressing plate 226. The lower end of the pressing plate 226 is fixedly connected to the suction cup 227. The suction cup 227 is externally connected to an air pipe (not shown in the figure). The suction cup 227 is located directly above the placement rack 215. The suction cup 227 is connected to the multi-stage filtration component 3.

[0046] The multi-stage filtration component 3 includes an ultrafiltration tube 31, a slot 32, a filter paper placement rack 33, a threaded groove 34, an upper cover 35, and a card slot 36. The ultrafiltration tube 31 includes a centrifuge tube and a filter tube embedded inside the centrifuge tube.

[0047] A slot 32 is formed in the filter tube inside the ultrafiltration tube 31. The lower end of the filter paper placement rack 33 is in interference contact with the slot 32. A filter paper is detachably installed inside the filter paper placement rack 33. A threaded groove 34 is formed at the upper end of the filter paper placement rack 33. The threaded groove 34 is threadedly connected to the inside of the upper cover 35. The suction end of the suction cup 227 is suction-connected to the upper end of the upper cover 35. A plurality of card slots 36 are formed at equal intervals at the lower end of the filter paper placement rack 33. The plurality of card slots 36 are respectively clamped with a plurality of rebound plates 217.

[0048] When the utility model is in use, the ultrafiltration tube 31 is manually placed into the hole position inside the centrifuge 12 from the feeding hole position 14. The centrifuge 12 drives the ultrafiltration tube 31 to rotate to the working hole position 15. The filter paper placement rack 33 is manually placed on the plurality of rebound plates 217. Then the nucleic acid to be filtered is poured into the filter paper of the filter paper placement rack 33, so as to filter out the macromolecules in the nucleic acid, and prevent the macromolecular substances from adsorbing on the filter tube and blocking the pipe orifice of the filter tube. This is the primary filtration of the nucleic acid. The nucleic acid enters the ultrafiltration tube 31 from the filter paper and then undergoes secondary filtration in the ultrafiltration tube 31.

[0049] After that, the conveyor belt transports the upper cover 35 to the support platform 11. The first servo motor 211 is started, and the first servo motor 211 drives the connecting rod 212 to rotate. The connecting rod 212 drives the support plate 213 to rotate together above the upper cover 35 at the conveyor belt. Then, the electric cylinder 221 is started, and the electric cylinder 221 drives the spline shaft 224 to move downward. The spline shaft 224 drives the pressure plate 226 and the suction cup 227 to move downward until the suction cup 227 contacts the upper end of the upper cover 35. After sucking and holding the upper cover 35, the electric cylinder 221 drives the suction cup 227 to rotate back to the original position;

[0050] Then, the output end of the electric cylinder 221 drives the suction cup 227 to move downward, and the rebound plate 217 drives the upper cover 35 to move downward. The upper cover 35 contacts the outer side of the upper end of the filter paper placement rack 33. The second servo motor 223 is started, and the second servo motor 223 drives the spline sleeve 225 to rotate through the pulley assembly 222. The spline sleeve 225 drives the spline shaft 224 to rotate. The spline shaft 224 drives the pressure plate 226 and the suction cup 227 to rotate together. The suction cup 227 drives the upper cover 35 to rotate. The output end of the electric cylinder 221 continues to drive the suction cup 227 to move downward, so that the thread inside the upper cover 35 is inserted into the thread groove 34. The output end of the electric cylinder 221 continues to drive the upper cover 35 to move downward until the filter paper placement rack 33 is pushed between multiple rebound plates 217. Under the action of the torsion spring 216, the clamping groove 36 of the filter paper placement rack 33 is clamped with the rebound plate 217. The rotation and downward pressure of the suction cup 227 achieve the locking of the upper cover 35 and the thread groove 34. The elastic force of the torsion spring 216 on the rebound plate 217 is greater than the downward pressure when the upper cover 35 and the thread groove 34 are tightened. Therefore, the filter paper placement rack 33 will not move downward;

[0051] After the upper cover 35 and the filter paper placement rack 33 are combined, the electric cylinder 221 continues to drive the suction cup 227 to move downward. The suction cup 227 pushes the combined component of the upper cover 35 and the filter paper placement rack 33 to move downward inside the rebound plate 217. The lower end of the filter paper placement rack 33 is inserted into the slot 32, so that the filter paper placement rack 33 is in interference contact with the slot 32 to fix the filter paper placement rack 33, realizing the combination of the ultrafiltration tube 31, the filter paper placement rack 33, and the upper cover 35;

[0052] Repeat the above steps until the combination of the ultrafiltration tubes 31 in the centrifuge 12 is completed.

[0053] After multiple groups of ultrafiltration tubes 31, filter paper placement racks 33, and upper covers 35 are combined, the centrifuge 12 is started to rotate. The centrifuge 12 drives multiple groups of ultrafiltration tubes 31 to rotate together, so that the nucleic acid enters the centrifuge tube from the filter tube, thereby realizing the separation and purification of the nucleic acid;

[0054] After the centrifuge 12 stops rotating at high speed, the electric cylinder 221 is activated. The electric cylinder 221 drives the suction cup 227 to move downward. After the suction cup 227 sucks and closes the upper cover 35, the assembly of the ultrafiltration tube 31, the filter paper placement rack 33, and the upper cover 35 is extracted from the centrifuge 12. The electric cylinder 221 is activated, and the electric cylinder 221 drives the connecting rod 212 to drive the support plate 213 to rotate above the conveyor belt. The suction cup 227 releases the upper cover 35 to realize the discharging of the assembly of the equipment main body 1, the filter paper placement rack 33, and the upper cover 35.

[0055] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements will not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-stage ultrafiltration device, comprising a device body (1) for separating and purifying nucleic acids, characterized in that: The device body (1) is provided with a combined mechanism (2) for assisting in nucleic acid filtration and purification; It also includes a multi-stage filtering component (3), wherein the multi-stage filtering component (3) is used to perform multi-stage filtering on nucleic acids; The combination mechanism (2) comprises a moving assembly (21) for loading and unloading the multi-stage filter assembly (3) and a pressing assembly (22) for combining the multi-stage filter assembly (3); the moving assembly (21) and the pressing assembly (22) are both connected to the device body (1).

2. The multi-stage ultrafiltration device according to claim 1, characterized in that: The equipment body (1) comprises a support platform (11), a centrifuge (12), a fixed cover (13), a loading hole (14) and a working hole (15); the middle portion of the support platform (11) is fixedly connected to the fixed cover (13); the centrifuge (12) is located at the lower end of the fixed cover (13); and the side wall of the centrifuge (12) is rotatably connected to the fixed cover (13); and the fixed cover (13) is provided with a loading hole (14) and a working hole (15).

3. The multi-stage ultrafiltration device according to claim 2, characterized in that: The support platform (11) is connected to the moving component (21).

4. The multi-stage ultrafiltration device according to claim 3, characterized in that: The moving assembly (21) comprises a first servo motor (211), a connecting rod (212), a support plate (213), a fixing plate (214), a placement frame (215), a vortex spring (216) and a rebound plate (217); the first servo motor (211) is fixedly mounted on the lower left end of the support platform (11); the connecting rod (212) is fixedly mounted on the output end of the first servo motor (211); the upper end of the connecting rod (212) is connected to the support plate (213); 3), the fixing plate (214) is rotatably connected to the lower end side wall of the connecting rod (212), the fixing plate (214) is fixed to the support platform (11) by bolts, the placement frame (215) is fixedly installed on the right side of the fixing plate (214), one end of the vortex spring (216) is fixedly connected to the inner end of the placement frame (215), the other end of the vortex spring (216) is fixedly connected to one end of the rebound plate (217), and the rebound plate (217) is connected to the multi-stage filter assembly (3).

5. The multi-stage ultrafiltration device according to claim 4, characterized in that: A plurality of vortex springs (216) and rebound plates (217) are arranged at equal intervals.

6. The multi-stage ultrafiltration device according to claim 5, characterized in that: The multiple vortex springs (216) and rebound plates (217) are arranged in a circle.

7. The multi-stage ultrafiltration device according to claim 6, characterized in that: The support plate (213) is connected to the pressing assembly (22).

8. The multi-stage ultrafiltration device according to claim 7, characterized in that: The pressing assembly (22) comprises an electric cylinder (221), a pulley assembly (222), a second servo motor (223), a spline shaft (224), a spline sleeve (225), a pressing plate (226) and a suction cup (227); the electric cylinder (221) and the second servo motor (223) are both fixedly mounted on the upper end of the support plate (213); the electric cylinder (221) is located on the right side of the second servo motor (223); the output end of the electric cylinder (221) is rotatably connected to the spline shaft (224); the spline sleeve (225) is The spline shaft (225) is rotatably connected to the lower end of the support plate (213), the spline shaft (224) is slidably connected to the spline sleeve (225), the second servo motor (223) is transmission-connected to the spline sleeve (225) via a pulley assembly (222), the lower end of the spline shaft (224) is fixedly connected to a pressure plate (226), the lower end of the pressure plate (226) is fixedly connected to a suction cup (227), the suction cup (227) is located directly above the placement rack (215), and the suction cup (227) is connected to the multi-stage filter assembly (3).

9. The multi-stage ultrafiltration device according to claim 8, characterized in that: The multi-stage filtering assembly (3) comprises an ultrafiltration tube (31), a slot (32), a filter paper mounting frame (33), a threaded groove (34), an upper cover (35) and a clamping groove (36); the ultrafiltration tube (31) is provided with a slot (32) inside; the lower end of the filter paper mounting frame (33) is in interference contact with the slot (32); the upper end of the filter paper mounting frame (33) is provided with a threaded groove (34); the threaded groove (34) is connected to the internal thread of the upper cover (35); the suction end of the suction cup (227) is suction-connected to the upper end of the upper cover (35); the lower end of the filter paper mounting frame (33) is provided with a plurality of clamping grooves (36) at equal intervals; the plurality of clamping grooves (36) are respectively clamped to a plurality of rebound plates (217).

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