Cell sorting magnetic column
By designing a cell sorting magnetic column with matching pores, the problem of traditional magnetic beads being easily aspirated or missed is solved, the efficiency and accuracy of cell sorting are improved, and the operation process is simplified.
Patent Information
- Application Number
- CN202421984877.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-15
- Publication Date
- 2025-06-27
- Estimated Expiration
- 2034-08-15
AI Technical Summary
During the magnetic sorting process of cell, traditional magnetic beads are easily aspirated or missed, resulting in inefficient sorting and complex operation.
A cell sorting magnetic column is designed, with an equal distance matching hole on the outer wall of the cylinder, which is used to place multiple sets of magnetic beads. After sorting is completed, you only need to remove the cylinder as a whole to avoid missed or missed beads.
It improves the efficiency and accuracy of cell sorting, simplifies the operation process, and reduces the operational errors of staff.
Smart Images
Figure CN223033358U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell sorting, in particular to a cell sorting magnetic column. Background Art
[0002] The immunomagnetic bead cell sorting method can isolate cells with high purity from a complex cell mixture within a few minutes. The cells are specifically labeled with superparamagnetic microbeads. After magnetic labeling, these cells are passed through a sorting column placed in a strong and stable magnetic field. The matrix in the sorting column creates a high-gradient magnetic field, and the magnetically labeled cells are retained in the column while the unlabeled cells flow out. When the sorting column is removed from the magnetic field, the magnetically labeled cells retained in the column can be eluted, and thus two cell components, the labeled and unlabeled ones, can be completely obtained.
[0003] However, during the process of magnetic sorting of cells, since traditional magnetic beads are all poured into the interior of the test tube, after cell sorting, the staff still needs to take out the magnetic beads one by one. At the same time, during the sorting process, it is very easy for the staff to suck the magnetic beads away from the test tube when taking the supernatant. Therefore, there is an urgent need for a cell sorting magnetic column to solve the above problems. Summary of the Utility Model
[0004] The purpose of the utility model is to solve the deficiencies existing in the prior art and propose a cell sorting magnetic column. Its advantages are that by placing multiple groups of magnetic beads in the matching holes opened on the circumferential outer wall of the column body, after cell sorting, only the whole column body needs to be taken out. At the same time, the magnetic beads arranged in the matching holes on its outer wall will not run out of the holes, so there is no situation where the staff sucks away the magnetic beads when taking the supernatant of the test tube, improving the efficiency of cell sorting and meeting the usage requirements of people.
[0005] In order to achieve the above purpose, the utility model adopts the following technical scheme:
[0006] A cell sorting magnetic column includes a column body. The circumferential outer wall of the column body is provided with equally spaced circularly distributed matching holes, and magnetic beads are arranged inside the matching holes;
[0007] The top outer wall of the column body is provided with a feed port, and a sealing plug is threadedly connected inside the feed port.
[0008] Through the above technical scheme: by placing multiple groups of magnetic beads in the matching holes opened on the circumferential outer wall of the column body, after cell sorting, only the whole column body needs to be taken out. At the same time, the magnetic beads arranged in the matching holes on its outer wall will not run out of the holes, so there is no situation where the staff sucks away the magnetic beads when taking the supernatant of the test tube, improving the efficiency of cell sorting and meeting the usage requirements of people.
[0009] The present utility model is further configured such that a surrounding ring plate is fixedly connected to the inner circumferential wall of the column body, and a storage area is formed between the surrounding ring plate and the inner circumferential wall of the column body.
[0010] Through the above technical solution: The bead is rolled into the storage area through the surrounding ring plate, and then rolled into the mating hole from the storage area again, realizing the installation work of the bead.
[0011] The present utility model is further configured such that a rotating block is fixedly connected to the outer wall of the top of the sealing plug, and anti-slip grooves are formed on the outer circumferential wall of the rotating block.
[0012] Through the above technical solution: By rotating the rotating block, the sealing plug can seal the feeding port, avoiding the leakage of beads.
[0013] The present utility model is further configured such that a retaining ring plate is provided inside the column body, and the retaining ring plates are equidistantly distributed from top to bottom inside the column body.
[0014] Through the above technical solution: The beads falling from the feeding port can be stably rolled into the interior of the mating hole.
[0015] The present utility model is further configured such that a through groove is formed in the middle of the surrounding ring plate, the cross section of the surrounding ring plate is an isosceles trapezoid, and the retaining ring plate is located at the through groove at the top of the surrounding ring plate.
[0016] Through the above technical solution: It is avoided that the surrounding ring plate is blocked and limited by the retaining ring plate during the removal process.
[0017] The present utility model is further configured such that a round hole is formed in the top of the retaining ring plate, the inner diameter of the round hole of the retaining ring plate gradually decreases from top to bottom, and a diversion ring groove is formed in the inner circumferential wall of the column body.
[0018] Through the above technical solution: It plays a good guiding role for the beads to roll from the outer wall of the surrounding ring plate into the mating hole, and at the same time, it is also convenient to pour out the beads from the column body for subsequent processing.
[0019] The present utility model is further configured such that a reinforcing rod is fixedly connected to the outer circumferential wall of the retaining ring plate, one end of the reinforcing rod away from the retaining ring plate is fixedly connected to another retaining ring plate, a support column is fixedly connected to the outer wall of the top of the retaining ring plate, and a handle is fixedly connected to one end of the support column.
[0020] Through the above technical solution: It is convenient for the staff to quickly disassemble or install the retaining ring plate.
[0021] The utility model is further configured such that a fixing column is fixedly connected to the inner wall of the bottom of the column body. A limiting disc is fixedly connected to the circumferential outer wall of the fixing column. The bottom outer wall of the retaining ring plate is in contact with the top outer wall of the limiting disc. One end of the fixing column passes through the round hole at the top of the limiting disc.
[0022] Through the above technical solutions, a good blocking and limiting effect can be achieved on the retaining ring plate, ensuring that the retaining ring plate can be stably installed inside the column body.
[0023] The beneficial effects of the utility model are as follows:
[0024] 1. A cell sorting magnetic column. By providing a column body, magnetic beads and a matching hole, by placing multiple groups of magnetic beads in the matching holes opened on the circumferential outer wall of the column body, after cell sorting is completed, only the whole column body needs to be taken out. At the same time, the magnetic beads arranged in the matching holes on its outer wall will not run out of the holes, so there is no situation where the magnetic beads are sucked away by the staff when taking the supernatant of the test tube, improving the efficiency of cell sorting and meeting the usage requirements of people.
[0025] 2. A cell sorting magnetic column. By providing a retaining ring plate, a support column and a reinforcing rod, before the staff puts magnetic beads into the column body, multiple groups of retaining ring plates can be first placed inside it. Then the staff puts magnetic beads into the column body through the feeding port. At this time, the magnetic beads will fall into the ring plate through the blocking effect of the retaining ring, and then roll into the matching hole through the ring plate, realizing the installation work of the magnetic beads. At the same time, the inner diameter of the round hole of the retaining ring plate gradually decreases from top to bottom, making the specifications of the retaining ring plate larger and larger from top to bottom, ensuring that an appropriate amount of magnetic beads can be filled in each matching hole.
[0026] 3. A cell sorting magnetic column. By providing a diversion ring groove, it plays a good guiding role for the magnetic beads to roll from the outer wall of the ring plate into the matching hole, and it is also convenient to pour out and process the magnetic beads from the column body later. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 FIG. 1 is a schematic diagram of the overall structure of a cell sorting magnetic column proposed by the present utility model;
[0028] Figure 2 FIG. 2 is a schematic diagram of the split structure of the column body of a cell sorting magnetic column proposed by the present utility model;
[0029] Figure 3 FIG. 3 is a schematic diagram of the overall half-sectional structure of a cell sorting magnetic column proposed by the present utility model;
[0030] Figure 4 FIG. 4 is a schematic diagram of the half-sectional plane structure of a cell sorting magnetic column proposed by the present utility model.
[0031] In the figure: 1. Cylinder; 2. Magnetic bead; 3. Fitting hole; 4. Rotating block; 5. Feeding port; 6. Sealing plug; 7. Support column; 8. Stop ring plate; 9. Fixed column; 10. Handle; 11. Diversion ring groove; 12. Surrounding ring plate; 13. Reinforcing rod; 14. Storage area; 15. Limit disk. Detailed implementation mode
[0032] The technical solution of this patent will be further described in detail below in combination with the specific implementation mode.
[0033] The embodiments of this patent are described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions from beginning to end. The embodiments described below by referring to the drawings are exemplary and are only used to explain this patent and should not be construed as a limitation of this patent.
[0034] In the description of this patent, it should be understood that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this patent.
[0035] In the description of this patent, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", "connection", "setting" should be understood in a broad sense. For example, it can be fixedly connected and set, or detachably connected and set, or integrally connected and set. For those of ordinary skill in the art, the specific meanings of the above terms in this patent can be understood according to specific circumstances.
[0036] Refer to Figures 1-4 , a cell sorting magnetic column, including a cylinder 1, on the circumferential outer wall of the cylinder 1, fitting holes 3 are provided at equal distances and distributed in a circular shape, and magnetic beads 2 are arranged inside the fitting holes 3;
[0037] On the top outer wall of the cylinder 1, a feeding port 5 is provided, and a sealing plug 6 is threadedly connected inside the feeding port 5.
[0038] Specifically, a surrounding ring plate 12 is fixedly connected to the circumferential inner wall of the cylinder 1. The surrounding ring plate 12 and the circumferential inner wall of the cylinder 1 form a storage area 14. By rolling into the storage area 14 through the surrounding ring plate 12 and then rolling into the fitting hole 3 again from the storage area 14, the installation work of the magnetic beads 2 is realized.
[0039] Specifically, a rotating block 4 is fixedly connected to the outer wall of the top of the sealing plug 6, and an anti-slip groove is provided on the circumferential outer wall of the rotating block 4. By rotating the rotating block 4, the sealing plug 6 can seal the feed port 5 to prevent the magnetic beads 2 from leaking.
[0040] Specifically, a retaining ring plate 8 is arranged inside the cylinder 1, and the retaining ring plates 8 are equidistantly distributed from top to bottom inside the cylinder 1, so that the magnetic beads 2 falling from the feed port 5 can stably roll into the inner part of the fitting hole 3.
[0041] Specifically, a through groove is provided in the middle of the surrounding ring plate 12, and the cross-section of the surrounding ring plate 12 is an isosceles trapezoid. The retaining ring plate 8 is located at the through groove at the top of the surrounding ring plate 12 to prevent the surrounding ring plate 12 from being blocked and limited by the retaining ring plate 8 during the removal process.
[0042] Specifically, a round hole is provided in the top of the retaining ring plate 8, and the inner diameter of the round hole of the retaining ring plate 8 gradually decreases from top to bottom. A diversion ring groove 11 is provided on the circumferential inner wall of the cylinder 1, which plays a good guiding role for the magnetic beads 2 to roll from the outer wall of the surrounding ring plate 12 into the fitting hole 3, and also facilitates the subsequent pouring out of the magnetic beads 2 from the cylinder 1 for treatment.
[0043] Specifically, a reinforcing rod 13 is fixedly connected to the circumferential outer wall of the retaining ring plate 8, and one end of the reinforcing rod 13 away from the retaining ring plate 8 is fixedly connected to another retaining ring plate 8. A support column 7 is fixedly connected to the outer wall of the top of the retaining ring plate 8, and a handle 10 is fixedly connected to one end of the support column 7, which facilitates the staff to quickly disassemble or install the retaining ring plate 8.
[0044] Specifically, a fixed column 9 is fixedly connected to the bottom inner wall of the cylinder 1, a limiting disk 15 is fixedly connected to the circumferential outer wall of the fixed column 9, the bottom outer wall of the retaining ring plate 8 is in contact with the top outer wall of the limiting disk 15, and one end of the fixed column 9 passes through the round hole at the top of the limiting disk 15, which can play a good role in blocking and limiting the retaining ring plate 8 and ensure that the retaining ring plate 8 can be stably installed inside the cylinder 1.
[0045] Working principle: Before the staff puts the magnetic beads 2 into the column 1, multiple sets of retaining ring plates 8 can be placed inside it first. Then, the staff puts the magnetic beads 2 into the column 1 through the feed port 5. At this time, the magnetic beads 2 will fall into the ring plate 12 through the blocking effect of the retaining ring, and then roll into the mating hole 3 through the ring plate 12, realizing the installation of the magnetic beads 2. At the same time, the inner diameter of the circular hole of the retaining ring plate 8 gradually decreases from top to bottom, so that the specifications of the retaining ring plate 8 become larger and larger from top to bottom, ensuring that an appropriate amount of magnetic beads 2 can be filled in each mating hole 3. Then, by placing multiple sets of magnetic beads 2 in the mating holes 3 opened on the circumferential outer wall of the column 1, after cell sorting is completed, only the whole column 1 needs to be taken out. At the same time, the magnetic beads 2 arranged in the mating holes 3 on its outer wall will not run out of the holes, and there will be no situation where the staff sucks away the magnetic beads 2 when taking the supernatant of the test tube, improving the efficiency of cell sorting and meeting people's usage requirements.
[0046] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.
Claims
1. A cell sorting magnetic column, comprising a column (1), characterized in that: The outer circumferential wall of the column (1) is provided with matching holes (3) which are equidistantly distributed in a circular pattern, and magnetic beads (2) are arranged inside the matching holes (3); A feed port (5) is provided on the top outer wall of the column (1), and a sealing plug (6) is connected to the inner thread of the feed port (5).
2. A cell sorting magnetic column according to claim 1, characterized in that: A ring plate (12) is fixedly connected to the circumferential inner wall of the column (1), and the ring plate (12) and the circumferential inner wall of the column (1) form a storage area (14).
3. A cell sorting magnetic column according to claim 2, characterized in that: The top outer wall of the sealing plug (6) is fixedly connected to a rotating block (4), and the circumferential outer wall of the rotating block (4) is provided with an anti-slip groove.
4. A cell sorting magnetic column according to claim 3, characterized in that: A baffle plate (8) is arranged inside the column (1), and the baffle plates (8) are distributed at equal distances from top to bottom inside the column (1).
5. A cell sorting magnetic column according to claim 4, characterized in that: A through groove is provided in the middle of the surrounding plate (12); the cross section of the surrounding plate (12) is an isosceles trapezoid; and the baffle plate (8) is located at the through groove at the top of the surrounding plate (12).
6. A cell sorting magnetic column according to claim 5, characterized in that: A circular hole is provided on the top of the baffle plate (8), the inner diameter of the circular hole of the baffle plate (8) gradually decreases from top to bottom, and a guide ring groove (11) is provided on the circumferential inner wall of the column (1).
7. A cell sorting magnetic column according to claim 6, characterized in that: A reinforcing rod (13) is fixedly connected to the circumferential outer wall of the baffle plate (8), one end of the reinforcing rod (13) away from the baffle plate (8) is fixedly connected to another baffle plate (8), and a support column (7) is fixedly connected to the top outer wall of the baffle plate (8), and a handle (10) is fixedly connected to one end of the support column (7).
8. A cell sorting magnetic column according to claim 7, characterized in that: The bottom inner wall of the column (1) is fixedly connected to a fixing column (9), the circumferential outer wall of the fixing column (9) is fixedly connected to a limiting plate (15), the bottom outer wall of the retaining ring plate (8) is in contact with the top outer wall of the limiting plate (15), and one end of the fixing column (9) passes through the circular hole at the top of the limiting plate (15).