Inoculation tool for evaluating preservative efficiency of cosmetics

Abstract

The utility model discloses an inoculation tool for evaluating the anticorrosion efficiency of cosmetics, which comprises an inoculation wire, the inoculation wire comprises a first straight wire, a second straight wire, a third straight wire and a fourth straight wire which are sequentially connected and positioned on the same plane, and the second straight wire, the third straight wire and the fourth straight wire form a U-shaped structure. And the included angle between the extension line of the first straight wire and the extension line of the third straight wire is 40-50 degrees. The inoculation tool is applied to marking of a porcelain bead strain preservation method, the front end of the inoculation wire is arranged to be of a U-shaped structure, a porcelain bead can penetrate into the fourth straight wire from the tail end of the fourth straight wire and then be stabilized in the third straight wire section, the porcelain bead is limited through the fourth straight wire and the second straight wire, and the situation that the marking effect is affected due to the fact that the porcelain bead slides off or moves in a large range is avoided. During scribing operation, the third straight wire is parallel to the plane of the culture medium, and the porcelain bead rotates around the third straight wire, fully contacts the culture medium and does not pierce the culture medium, and meanwhile, the passage effect is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of inoculation tools, and particularly relates to an inoculation tool for evaluating the anti-corrosion efficacy of cosmetics. Background Art

[0002] The anti-corrosion efficacy evaluation test of cosmetics is a test method in which a certain amount of microorganisms are artificially contaminated into cosmetics to simulate the possible contamination situations of cosmetics, and the viable bacteria count is detected at regular intervals, and the efficacy of the anti-corrosion system of cosmetics is evaluated according to the change of the viable bacteria count. The microorganisms used in the anti-corrosion efficacy evaluation test of cosmetics are usually the third-generation strains, which are obtained by the laboratory personnel through three times of three-zone streaking from the purchased standard strains, and are usually stored in porcelain bead cryotubes. The porcelain bead bacteria preservation method has a better bacteria preservation effect than the traditional preservation method, is convenient to use, can reduce the damage of the bacterial strain caused by repeated freezing and thawing, and is the most widely used bacteria preservation method at present.

[0003] Generally, a porcelain bead cryotube contains 12 frosted porcelain beads and a bacteria preservation solution. Pick the bacteria in the logarithmic growth phase and transfer them to the cryotube, invert and mix them up and down. At this time, the bacteria will be evenly distributed in the preservation solution. Use a pipette to suck out the preservation solution. At this time, under the action of surface tension, a layer of preservation solution will cover the surface of each porcelain bead, and the bacteria are preserved in this layer of preservation solution. Transfer the cryotube containing the bacteria to a -80 °C refrigerator for long-term storage (usually can be stored for about 1 year). When the bacteria need to be taken, take out the cryotube from the refrigerator, gently shake it to disperse the porcelain beads, pick one porcelain bead and inoculate it on the culture medium plate for streaking culture, or inoculate it into the liquid culture medium for shaking culture. After inoculation, quickly put the cryotube back into the refrigerator.

[0004] At present, the most commonly used tools for picking porcelain beads for streaking in the laboratory are inoculation needles, inoculation loops and inoculation hooks respectively. When the inoculation needle picks up the porcelain bead for streaking, the porcelain bead is easy to slide off the tip of the needle, and the inoculation needle is easy to pierce the culture medium, which is not conducive to subculture; when the inoculation loop holds up the porcelain bead for streaking, in order to hold up the porcelain bead, the diameter of the inoculation loop needs to be smaller than the diameter of the porcelain bead. In actual streaking, only a small part of the porcelain bead protruding from the bottom of the inoculation loop contacts the culture medium, or even the porcelain bead does not contact the culture medium, but relies on the small amount of bacteria contaminated on the inoculation loop for streaking, or sometimes the subculture fails, and the subculture effect is unstable; when the inoculation hook picks up the porcelain bead for streaking, it is more test the streaking technique and angle, and the porcelain bead is easy to fall off from the opening, affecting the streaking effect and streaking efficiency. It can be seen that it is of great significance to design an inoculation tool with convenient operation and stable subculture effect. Summary of the Invention

[0005] The technical problem to be solved by the present utility model is to provide an inoculation tool for evaluating the anti-corrosion efficacy of cosmetics, which is applied to the streaking of the porcelain bead strain preservation method, and solves the problems that the porcelain beads are easy to fall off, the operation difficulty is large, the culture medium is easy to be damaged, and the subculture effect is unstable when using the existing inoculation tools for streaking.

[0006] To solve the above technical problems, the present utility model provides an inoculation tool for evaluating the anti-corrosion efficacy of cosmetics, including an inoculation wire. The inoculation wire includes a first straight wire, a second straight wire, a third straight wire, and a fourth straight wire that are sequentially connected and in the same plane. The second straight wire, the third straight wire, and the fourth straight wire form a U-shaped structure, and the included angle between the extension line of the first straight wire and the extension line of the third straight wire is 40-50°.

[0007] The inoculation tool of the present utility model is applied to the streaking of the porcelain bead strain preservation method. The front end of the inoculation wire is combined into a U-shaped structure by the second straight wire, the third straight wire, and the fourth straight wire, which is convenient for the porcelain bead to penetrate from the end of the fourth straight wire and be stable in the section of the third straight wire, and the porcelain bead is limited by the fourth straight wire and the second straight wire to prevent the porcelain bead from slipping or moving greatly and affecting the streaking effect. During the streaking operation, the third straight wire is parallel to the culture medium plane, and the porcelain bead rotates around the third straight wire to fully contact the culture medium to ensure the subculture effect. The included angle between the extension line of the first straight wire and the extension line of the third straight wire is 40-50°. When subculturing, when the third straight wire is parallel to the culture medium plane, the first straight wire is inclined at an angle of 40-50° with the plane, which conforms to the streaking habit of the experimenter and makes the streaking more efficient and convenient.

[0008] Further, it also includes a handle and a connecting piece. One end of the first straight wire far from the second straight wire is inserted into the handle, and the first straight wire and the handle are fixedly connected by the connecting piece. The handle is provided to facilitate the experimenter to hold and operate.

[0009] Further, the included angle between the first straight wire and the second straight wire is 100-125°.

[0010] Further, an arc chamfer is provided at the included angle between the third straight wire and the fourth straight wire to facilitate the porcelain bead to smoothly enter the section of the third straight wire after passing through the fourth straight wire, and no chamfer is provided at the included angle between the second straight wire and the third straight wire to prevent the porcelain bead from entering the section of the second straight wire.

[0011] Further, the chamfer radius is 0.5±0.05mm, and the chamfer angle is 80-100°.

[0012] Further, the length of the second straight wire is 3.5±0.2mm.

[0013] Further, the length of the third straight wire is 3±0.2mm.

[0014] Furthermore, the length of the fourth straight wire is 3 ± 0.2 mm.

[0015] Furthermore, the inoculation wire is of an integrally formed structure.

[0016] Furthermore, the material of the inoculation wire is nickel-chromium alloy.

[0017] Advantages of the present utility model:

[0018] The inoculation tool of the present utility model is applied to the scribing of the porcelain bead strain preservation method. The front end of the inoculation wire is set as a U-shaped structure, which is convenient for the porcelain bead to penetrate from the end of the fourth straight wire and be stabilized in the third straight wire section. The fourth straight wire and the second straight wire limit the porcelain bead to prevent the porcelain bead from slipping or moving largely, which affects the scribing effect. During the scribing operation, the third straight wire is parallel to the culture medium plane, and the porcelain bead rotates around the third straight wire, fully contacting the culture medium without piercing the culture medium, and at the same time ensuring the subculture effect.

[0019] When the inoculation tool of the present utility model is subcultured, the third straight wire is parallel to the culture medium plane, and the angle between the first straight wire and the plane is inclined at 40 - 50°, which conforms to the scribing habit of experimental personnel, making the scribing more efficient and convenient. Description of the Drawings

[0020] Figure 1 is a scribing schematic diagram of the inoculation tool for evaluating the anti-corrosion efficacy of cosmetics of the present utility model;

[0021] Figure 2 is a schematic diagram of the inoculation wire structure of the inoculation tool for evaluating the anti-corrosion efficacy of cosmetics of the present utility model;

[0022] Explanation of the reference numerals in the figures:

[0023] 1. Inoculation wire, 11. First straight wire, 12. Second straight wire, 13. Third straight wire, 14. Fourth straight wire;

[0024] 2. Porcelain bead; 3. Culture medium; 4. Handle; 5. Connector. Detailed Embodiment

[0025] The following further illustrates the present utility model in conjunction with the drawings and specific embodiments, so that those skilled in the art can better understand the present utility model and be able to implement it, but the embodiments cited do not limit the present utility model.

[0026] This embodiment provides an inoculation tool for evaluating the anti-corrosion efficacy of cosmetics. The scribing schematic diagram of the inoculation tool on the surface of the culture medium is as Figure 1As shown in the figure, the inoculation tool includes an inoculation wire 1. The inoculation wire 1 includes a first straight wire 11, a second straight wire 12, a third straight wire 13, and a fourth straight wire 14 that are sequentially connected and in the same plane. The second straight wire 12, the third straight wire 13, and the fourth straight wire 14 form a U-shaped structure, and the included angle between the extension line of the first straight wire 11 and the extension line of the third straight wire 13 is 40 - 50°.

[0027] This embodiment of the inoculation tool is applied to the scribing of the porcelain bead strain preservation method. The front end of the inoculation wire 1 is combined into a U-shaped structure by the second straight wire 12, the third straight wire 13, and the fourth straight wire 14. When using the inoculation tool to pick up the porcelain bead 2 in the porcelain bead cryopreservation tube, the porcelain bead 2 penetrates from the end of the fourth straight wire 14 and is stabilized in the section of the third straight wire 13, and the porcelain bead 2 is limited by the fourth straight wire 14 and the second straight wire 12 to prevent the porcelain bead 2 from slipping or moving largely, which affects the scribing effect.

[0028] During the scribing operation, the third straight wire 13 is parallel to the plane of the culture medium 3, the porcelain bead 2 contacts the culture medium 3, and the inoculation tool is moved in a direction perpendicular to the third straight wire 13. The porcelain bead 2 rotates around the third straight wire 13 to fully contact the culture medium 3 to ensure the subculture effect. The included angle between the extension line of the first straight wire 11 and the extension line of the third straight wire 13 is 40 - 50°. During subculture, when the third straight wire 13 is parallel to the plane of the culture medium 3, the included angle between the first straight wire 11 and the plane is inclined at 40 - 50°, which conforms to the scribing habit of the experimenter and makes the scribing more efficient and convenient.

[0029] Specifically, an arc chamfer is provided at the included angle between the third straight wire 13 and the fourth straight wire 14. The chamfer radius is 0.5 ± 0.05 mm, and the chamfer angle is 80 - 100°. This is convenient for the porcelain bead 2 to smoothly enter the section of the third straight wire 13 after passing through the fourth straight wire 14, and there is no chamfer at the included angle between the second straight wire 12 and the third straight wire 13 to prevent the porcelain bead 2 from entering the section of the second straight wire 12.

[0030] Preferably, it further includes a handle 4 and a connecting piece 5. One end of the first straight wire 11 far from the second straight wire 12 is inserted into the handle 4, and the first straight wire 11 and the handle 4 are fixedly connected through the connecting piece 5. The handle 4 is provided to facilitate the experimenter's hand-held operation.

[0031] Preferably, the included angle between the first straight wire 11 and the second straight wire 12 is 100 - 125°, the length of the second straight wire 12 is 3.5 ± 0.2 mm, the length of the third straight wire 13 is 3 ± 0.2 mm, and the length of the fourth straight wire 14 is 3 ± 0.2 mm. Through the design of the size and angle of the inoculation wire 1, it is convenient for the scribing operation of the porcelain bead 2 and improves the subculture effect.

[0032] Preferably, the inoculation wire 1 is an integrally formed structure, and the material of the inoculation wire 1 is nickel-chromium alloy.

[0033] In summary, the inoculation tool of the present utility model is applied to the streaking of the porcelain bead strain preservation method. The front end of the inoculation wire is set as a U-shaped structure, which facilitates the porcelain bead to penetrate from the end of the fourth straight wire and be stabilized in the third straight wire section, and limits the position of the porcelain bead through the fourth straight wire and the second straight wire, avoiding the porcelain bead from slipping or moving largely and affecting the streaking effect. During the streaking operation, the third straight wire is parallel to the culture medium plane, and the porcelain bead rotates around the third straight wire, fully contacting the culture medium without piercing the culture medium, while ensuring the subculture effect. When the inoculation tool of the present utility model is subcultured, when the third straight wire is parallel to the culture medium plane, the included angle between the first straight wire and the plane is inclined at 40-50°, which conforms to the streaking habit of the experimenter and makes the streaking more efficient and convenient.

[0034] The above-described embodiments are only preferred embodiments cited to fully illustrate the present utility model, and the protection scope of the present utility model is not limited thereto. Equivalent substitutions or transformations made by those skilled in the art on the basis of the present utility model are all within the protection scope of the present utility model. The protection scope of the present utility model is subject to the claims.

Claims

1. An inoculation tool for evaluating the preservative efficacy of cosmetics, characterized in that: The invention comprises an inoculation wire, wherein the inoculation wire comprises a first straight wire, a second straight wire, a third straight wire and a fourth straight wire which are sequentially connected and located in the same plane, the second straight wire, the third straight wire and the fourth straight wire form a "U"-shaped structure, and the angle between the extension line of the first straight wire and the extension line of the third straight wire is 40-50°.

2. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: It also includes a handle and a connecting piece. One end of the first straight wire away from the second straight wire is inserted into the handle. The first straight wire and the handle are fixedly connected by the connecting piece.

3. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The included angle between the first straight wire and the second straight wire is 100-125°.

4. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: An arc chamfer is provided at the included angle between the third straight wire and the fourth straight wire.

5. The inoculation tool for evaluating the antiseptic efficacy of cosmetics according to claim 4, characterized in that: The chamfer radius is 0.5±0.05mm and the chamfer angle is 80-100°.

6. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The length of the second straight wire is 3.5±0.2 mm.

7. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The length of the third straight wire is 3±0.2 mm.

8. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The length of the fourth straight wire is 3±0.2 mm.

9. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The inoculation wire is an integrally formed structure.

10. The inoculation tool for evaluating the preservative efficacy of cosmetics according to claim 1, characterized in that: The material of the inoculation wire is nickel-chromium alloy.

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