Sealed separation equipment for adipose-derived stem cells

Abstract

The utility model relates to the technical field of separation equipment, in particular to adipose-derived stem cell sealing separation equipment. According to the technical scheme, the cutting device comprises a bottom plate and further comprises a cutting barrel, the cutting barrel is fixedly installed on the bottom plate, a feeding pipe is fixedly installed on one side of the cutting barrel, a first air cylinder is fixedly installed on the cutting barrel, an electric rotating disc is fixedly installed on a piston rod of the first air cylinder, and a blade is fixedly installed at the bottom of the electric rotating disc; a sieve plate is slidably mounted in the cutting barrel, a flow guide groove is fixedly formed in the bottom of the sieve plate, and vibration motors are fixedly mounted on the two sides of the flow guide groove and fixedly connected with the sieve plate. The adipose-derived stem cells are fully cut, so that the separase is fully and uniformly contacted with the adipose-derived stem cells, the separation rate and effect are improved, and the practicability is high.

Description

Technical Field

[0001] The utility model relates to the technical field of separation equipment, in particular to a sealed separation equipment for adipose stem cells. Background Art

[0002] Adipose stem cells are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue. Adipose tissue is rich in reserves in the human body. A large number of adipose stem cells obtained from it by liposuction have the potential of self-renewal, proliferation and multi-directional differentiation. They can differentiate into adipocytes, chondrocytes, muscle cells, osteoblasts, nerve cells, glial cells and pancreatic islet cells. Moreover, they can secrete a variety of angiogenesis-promoting factors and anti-apoptotic factors to have anti-inflammatory and antioxidant effects, resist the damage of oxygen free radicals, and are expected to become a source of stem cells for repairing damaged tissues and organs.

[0003] In the process of separating adipose stem cells, it is necessary to cut adipose cells into small parts and then add enzymes for cell separation. Since adipose tissue is relatively loose and some adipose tissue is relatively dense, when cutting loose adipose tissue, the cutting will cause uneven sizes. The added cell separation enzyme cannot fully and evenly contact with adipose cells. Smaller adipose cells are more easily affected by the enzyme, while larger adipose cells may require a longer time of enzyme treatment to be separated, which affects the separation effect and separation rate. Therefore, this application proposes a sealed separation equipment for adipose stem cells. Summary of the Invention

[0004] The purpose of the utility model is to propose a sealed separation equipment for adipose stem cells aiming at the problem that adipose cells cannot fully and evenly contact with enzymes in the background art.

[0005] The technical solution of the utility model: The sealed separation equipment for adipose stem cells includes a bottom plate, and further includes:

[0006] A cutting barrel, the cutting barrel is fixedly installed on the bottom plate, a feed pipe is fixedly installed on one side of the cutting barrel, a first cylinder is fixedly installed on the cutting barrel, a piston rod of the first cylinder is fixedly installed with an electric turntable, a blade is fixedly installed at the bottom of the electric turntable, a sieve plate is slidably installed in the cutting barrel, a diversion groove is fixedly installed at the bottom of the sieve plate, vibration motors are fixedly installed on both sides of the diversion groove, the vibration motors are fixedly connected with the sieve plate, a first diversion pipe is fixedly installed at the bottom of the diversion groove, a culture chamber is fixedly installed on the bottom plate, the culture chamber is communicated with the first diversion pipe, and an auxiliary device for quickly and evenly mixing adipose stem cells and separation enzymes is arranged in the culture chamber.

[0007] Optionally, the auxiliary device includes a second cylinder fixedly installed on one side of the culture chamber. A slide plate is slidably installed in the culture chamber. A diversion chamber is fixedly installed at the bottom of the slide plate. The diversion chamber is a hollow cavity. A plurality of nozzles arranged at equal distances are fixedly installed at the bottom of the diversion chamber. A liquid storage tank is fixedly installed on the culture chamber. A second diversion pipe is fixedly installed on one side of the liquid storage tank. The second diversion pipe is communicated with the diversion chamber. Fixing plates are fixedly installed on both sides of the slide plate. A plurality of stirring rods arranged at equal distances are fixedly installed at the bottom of the fixing plates.

[0008] Optionally, bumps are fixedly installed on both sides of the slide plate, and sliding grooves are formed inside the culture chamber.

[0009] Optionally, an installation groove is formed on the inner wall of the cutting barrel. A connecting block is slidably installed in the installation groove. The connecting block is fixedly connected with the sieve plate. A spring is fixedly installed in the installation groove. One end of the spring is fixedly connected with the connecting block.

[0010] Optionally, a mounting plate is fixedly installed at the bottom of the electric turntable. The blade is fixedly connected with the mounting plate.

[0011] Optionally, the bumps are located in the sliding grooves. A connecting pipe is fixedly installed on one side of the liquid storage tank. The connecting pipe is communicated with the culture chamber.

[0012] Compared with the prior art, the utility model has the following beneficial technical effects:

[0013] 1. By transporting adipose stem cells into the cutting barrel, starting the first cylinder, the piston rod of the first cylinder drives the electric turntable to perform reciprocating motion in the vertical direction in the cutting barrel. At the same time, starting the electric turntable, the electric turntable drives the blade to rotate. The blade fully cuts and shreds the tissue stem cells. Cooperating with the vibration motor, the vibration motor drives the sieve plate to vibrate, vibrating the adipose stem cells that can pass through the sieve plate above into the diversion groove, and then being transported to the culture chamber through the first diversion pipe, realizing the full cutting and collection of adipose stem cells;

[0014] 2. By starting the second cylinder to drive the slide plate to perform reciprocating motion horizontally, the enzyme in the liquid storage tank is transported to the diversion chamber through the second diversion pipe, and then sprayed out by the nozzles at the bottom of the diversion chamber. As the slide plate moves continuously, the nozzles evenly spray the separation enzyme on the adipose stem cells in the culture chamber, and the stirring rods stir the adipose stem cells, realizing the full contact between the separation enzyme and the adipose stem cells, and improving the separation effect and efficiency.

[0015] The utility model realizes the full cutting of adipose stem cells, enables the separation enzyme to be fully and evenly contacted with the adipose stem cells, improves the separation rate and effect, and has strong practicability. Description of the Drawings

[0016] Figure 1 Show the internal structure schematic diagram of an embodiment of the present utility model Figure 1 ;

[0017] Figure 2 Show the structural schematic diagram of an embodiment of the present utility model;

[0018] Figure 3 Show the internal structure schematic diagram of an embodiment of the present utility model Figure 2 ;

[0019] Figure 4 Show the structural schematic diagram of the skateboard, diversion cavity and stirring rod of an embodiment of the present utility model;

[0020] Figure 5 is Figure 1 the partial enlarged structural schematic diagram at A in

[0021] Reference numerals: 1, bottom plate; 2, cutting barrel; 3, first cylinder; 4, feed pipe; 5, liquid storage tank; 6, second cylinder; 7, connecting pipe; 8, culture chamber; 9, second diversion pipe; 10, electric turntable; 11, blade; 12, mounting plate; 13, sieve plate; 14, vibration motor; 15, diversion groove; 16, skateboard; 17, convex block; 18, fixing plate; 19, diversion cavity; 20, spray head; 21, stirring rod; 22, mounting groove; 23, spring; 24, connecting block; 25, first diversion pipe. Detailed implementation manners

[0022] The technical solutions of the present utility model will be further described below in conjunction with the accompanying drawings and specific embodiments. Embodiment

[0023] As Figures 1-3As shown in the figure, the adipose stem cell sealing and separation device proposed by the present utility model includes a bottom plate 1, and also includes a cutting barrel 2 for initially chopping adipose stem cells. The cutting barrel 2 is fixedly installed on the bottom plate 1. One side of the cutting barrel 2 is fixedly installed with a feed pipe 4, and a sealing plate is provided on the feed pipe 4 for sealing the cutting barrel 2. A first cylinder 3 is fixedly installed on the cutting barrel 2. The piston rod of the first cylinder 3 is fixedly installed with an electric turntable 10, and a blade 11 is fixedly installed at the bottom of the electric turntable 10. A sieve plate 13 is slidably installed in the cutting barrel 2. A diversion groove 15 is fixedly installed at the bottom of the sieve plate 13. Vibration motors 14 are fixedly installed on both sides of the diversion groove 15, and the vibration motors 14 are fixedly connected to the sieve plate 13. A first diversion pipe 25 is fixedly installed at the bottom of the diversion groove 15. A culture chamber 8 is fixedly installed on the bottom plate 1, and the culture chamber 8 is communicated with the first diversion pipe 25. Start the first cylinder 3, and the piston rod of the first cylinder 3 drives the electric turntable 10 to perform reciprocating motion in the vertical direction in the cutting barrel 2. At the same time, start the electric turntable 10, and the electric turntable 10 drives the blade 11 to rotate. As the blade 11 rotates, the blade 11 fully chops the tissue stem cells. Start the vibration motors 14, and the vibration motors 14 drive the sieve plate 13 to vibrate, vibrating the adipose stem cells that can pass through the sieve plate 13 above into the diversion groove 15, and then transporting them to the culture chamber 8 through the first diversion pipe 25. An auxiliary device for quickly and evenly mixing adipose stem cells and separation enzyme is provided in the culture chamber 8 to improve the separation efficiency and separation effect.

[0024] As Figures 1-4 shown, an auxiliary device is further included in this embodiment. The auxiliary device includes a second cylinder 6 fixedly installed on one side of the culture chamber 8. A slide plate 16 is slidably installed in the culture chamber 8. A diversion cavity 19 is fixedly installed at the bottom of the slide plate 16. The diversion cavity 19 is a cavity. A plurality of spray heads 20 arranged at equal distances are fixedly installed at the bottom of the diversion cavity 19. A liquid storage tank 5 for storing the enzyme used for adipose stem cell separation is fixedly installed on the culture chamber 8. A second diversion pipe 9 is fixedly installed on one side of the liquid storage tank 5, and a valve is provided on the second diversion pipe 9. The second diversion pipe 9 is communicated with the diversion cavity 19. Fixing plates 18 are fixedly installed on both sides of the slide plate 16, and a plurality of stirring rods 21 arranged at equal distances are fixedly installed at the bottom of the fixing plates 18. Start the second cylinder 6, and the second cylinder 6 drives the slide plate 16 to perform reciprocating motion horizontally. Open the valve on the second diversion pipe 9, and the enzyme in the liquid storage tank 5 is transported to the diversion cavity 19 through the second diversion pipe 9 and then sprayed out by the spray heads 20 at the bottom of the diversion cavity 19. As the slide plate 16 continuously moves, the spray heads 20 evenly spray the separation enzyme on the adipose stem cells in the culture chamber 8, and the stirring rods 21 stir the adipose stem cells, enabling the separation enzyme to be in full contact with the adipose stem cells, thereby improving the separation efficiency and effect.

[0025] As Figures 3-5As shown, bump 17 is fixedly installed on both sides of skateboard 16. A chute is provided inside culture chamber 8. An installation groove 22 is provided on the inner wall of cutting barrel 2. A connecting block 24 is slidably installed in installation groove 22. Connecting block 24 is fixedly connected to sieve plate 13. A spring 23 is fixedly installed in installation groove 22. One end of spring 23 is fixedly connected to connecting block 24.

[0026] As Figures 1-2 shown, a mounting plate 12 is fixedly installed at the bottom of electric turntable 10. Blade 11 is fixedly connected to mounting plate 12. Bump 17 is located inside the chute. A connecting pipe 7 is fixedly installed on one side of liquid storage tank 5. Connecting pipe 7 communicates with culture chamber 8.

[0027] Working principle: The adipose stem cells to be cut are transported from feed pipe 4 into cutting barrel 2. The sealing plate is closed. First cylinder 3 is started. The piston rod of first cylinder 3 drives electric turntable 10 to perform reciprocating motion in the vertical direction inside cutting barrel 2. At the same time, electric turntable 10 is started. Electric turntable 10 drives blade 11 to rotate. As blade 11 rotates, blade 11 fully cuts up the tissue stem cells. Vibration motor 14 is started. Vibration motor 14 drives sieve plate 13 to vibrate, vibrating the adipose stem cells that can pass through sieve plate 13 above into diversion groove 15, and then transporting them to culture chamber 8 through first diversion pipe 25. Second cylinder 6 is started. Second cylinder 6 drives skateboard 16 to perform reciprocating motion horizontally. The valve on second diversion pipe 9 is opened. The enzyme in liquid storage tank 5 is transported to diversion cavity 19 through second diversion pipe 9, and then sprayed out from nozzle 20 at the bottom of diversion cavity 19. As skateboard 16 continuously moves, nozzle 20 evenly sprays the separation enzyme on the adipose stem cells in culture chamber 8. Stirring rod 21 stirs the adipose stem cells, enabling the separation enzyme to come into full contact with the adipose stem cells, achieving full cutting of the adipose stem cells, enabling the separation enzyme to come into full and even contact with the adipose stem cells, improving the separation rate and effect, and having strong practicability.

[0028] The above specific embodiments are merely several alternative embodiments of the present utility model. Based on the technical solution of the present utility model and the relevant inspirations of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A sealed separation device for adipose stem cells, comprising a bottom plate (1), characterized in that: Also includes: A cutting barrel (2), the cutting barrel (2) being fixedly mounted on a bottom plate (1), a feeding pipe (4) being fixedly mounted on one side of the cutting barrel (2), a first cylinder (3) being fixedly mounted on the cutting barrel (2), an electric turntable (10) being fixedly mounted on the piston rod of the first cylinder (3), a blade (11) being fixedly mounted on the bottom of the electric turntable (10), a sieve plate (13) being slidably mounted in the cutting barrel (2), a guide groove (15) being fixedly mounted on the bottom of the sieve plate (13), a vibration motor (14) being fixedly mounted on both sides of the guide groove (15), the vibration motor (14) being fixedly connected to the sieve plate (13), a first guide pipe (25) being fixedly mounted on the bottom of the guide groove (15), a culture chamber (8) being fixedly mounted on the bottom plate (1), the culture chamber (8) being connected to the first guide pipe (25), and an auxiliary device for quickly and evenly mixing adipose stem cells and separation enzymes being arranged in the culture chamber (8).

2. The adipose stem cell sealing separation device according to claim 1, characterized in that: The auxiliary device comprises a second cylinder (6) fixedly mounted on one side of the culture bin (8); a slide plate (16) is slidably mounted in the culture bin (8); a flow guide cavity (19) is fixedly mounted at the bottom of the slide plate (16); the flow guide cavity (19) is a hollow cavity; a plurality of nozzles (20) arranged at equal distances are fixedly mounted at the bottom of the flow guide cavity (19); a liquid storage tank (5) is fixedly mounted on the culture bin (8); a second flow guide pipe (9) is fixedly mounted on one side of the liquid storage tank (5); the second flow guide pipe (9) is connected to the flow guide cavity (19); fixed plates (18) are fixedly mounted on both sides of the slide plate (16); a plurality of stirring rods (21) arranged at equal distances are fixedly mounted at the bottom of the fixed plates (18).

3. The adipose stem cell sealing separation device according to claim 2, characterized in that: Bumps (17) are fixedly mounted on both sides of the slide plate (16), and a slide groove is provided inside the culture chamber (8).

4. The adipose stem cell sealing separation device according to claim 1, characterized in that: The inner wall of the cutting barrel (2) is provided with a mounting groove (22), a connecting block (24) is slidably mounted in the mounting groove (22), the connecting block (24) is fixedly connected to the sieve plate (13), a spring (23) is fixedly mounted in the mounting groove (22), one end of the spring (23) is fixedly connected to the connecting block (24).

5. The adipose stem cell sealing separation device according to claim 1, characterized in that: A mounting plate (12) is fixedly mounted on the bottom of the electric turntable (10), and the blade (11) is fixedly connected to the mounting plate (12).

6. The adipose stem cell sealing separation device according to claim 3, characterized in that: The protrusion (17) is located in the slide groove, and a connecting pipe (7) is fixedly installed on one side of the liquid storage tank (5), and the connecting pipe (7) is connected to the culture chamber (8).

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