Multifunctional polypeptide synthesis reaction kettle convenient to operate

Abstract

The utility model relates to the technical field of reaction kettles, and provides a multifunctional polypeptide synthesis reaction kettle convenient to operate, which comprises a kettle body and a chassis, and the bottom of the kettle body is provided with an opening; the bottom disc is arranged at an opening in the bottom of the kettle body in a sealed mode, the upper end face of the bottom disc is a conical face with the middle sunken downwards, the bottom disc is provided with a filtrate outlet, and the filtrate outlet is located at the lowest position of the conical face of the bottom disc. According to the technical scheme, the problems that a synthetic reaction kettle in the prior art is complex in structure, and time and labor are wasted during discharging are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of reaction kettles, and specifically, to a multi-functional polypeptide synthesis reaction kettle with convenient operation. Background Art

[0002] Polypeptide is a bioactive substance related to various cell functions in organisms. Its molecular structure is between amino acids and proteins, and it is a compound formed by multiple amino acids combined through peptide bonds in a certain arrangement order. Polypeptide synthesis is a process of repeatedly adding amino acids. The solid-phase synthesis sequence generally proceeds from the C-terminal (carboxyl terminal) to the N-terminal (amino terminal); during the process of synthesizing polypeptides, semi-finished or finished products in the form of coarse sand are produced, and the unreacted reaction solution filters out, while the semi-finished or finished products remain on the filter screen for the next reaction. The synthesis reaction kettle installs a bottom valve at the central part of the chassis, and uses pneumatic opening and closing to achieve the transfer of materials after synthesis. The structure is complex, and a certain amount of additional gas source is required to supply the opening and closing of the bottom valve, and there are certain requirements for the pressure of the gas source, and an additional gas circuit needs to be added on site. Or the chassis is disassembled for manual discharging, which is time-consuming and laborious. Content of the Utility Model

[0003] The utility model provides a multi-functional polypeptide synthesis reaction kettle with convenient operation, which solves the problems of complex structure and time-consuming and laborious discharging of the synthesis reaction kettle in the related technology.

[0004] The technical solution of the utility model is as follows: A multi-functional polypeptide synthesis reaction kettle with convenient operation, which is characterized by comprising:

[0005] A kettle body, the bottom of the kettle body has an opening;

[0006] A chassis, the chassis is hermetically arranged at the opening at the bottom of the kettle body, the upper end surface of the chassis is a conical surface sunken downward in the middle, the chassis has a filtrate outlet, and the filtrate outlet is located at the lowest point of the conical surface of the chassis.

[0007] It further comprises a three-way valve, the three-way valve is arranged below the chassis, the three-way valve has an inert gas inlet, a filtrate discharge port and a communication port, and the communication port of the three-way valve is communicated with the filtrate outlet.

[0008] It further comprises:

[0009] A filter screen, the filter screen is arranged on the chassis and above the conical surface of the chassis;

[0010] A plunger valve, the plunger valve is arranged on the kettle body and outside the kettle body, the inlet end of the plunger valve is communicated with the inside of the kettle body, and the filter screen is between the highest point and the lowest point of the inlet end of the plunger valve.

[0011] The chassis has a heating cavity, and the heating cavity is located below the conical surface of the chassis. It further includes,

[0012] a heating medium input pipe, one end of the heating medium input pipe is located outside the chassis, and the other end of the heating medium input pipe communicates with the heating cavity;

[0013] a heating medium output pipe, one end of the heating medium output pipe communicates with the heating cavity, and the other end of the heating medium output pipe is located outside the chassis;

[0014] a temperature sensor, the temperature sensor is arranged on the kettle body, the temperature sensor has a detection end and a display end, the detection end of the temperature sensor is located inside the kettle body, and the display end of the temperature sensor is located outside the kettle body.

[0015] The chassis has a heat preservation cavity, and the heat preservation cavity is located below the heating cavity.

[0016] It further includes a strip-shaped sight glass, and the strip-shaped sight glass is arranged on the side wall of the kettle body.

[0017] It further includes a manhole sight glass, and the manhole sight glass is arranged on the top of the kettle body.

[0018] A pressure sensor interface and a rupture disc interface are arranged on the top of the kettle body.

[0019] It further includes a manual discharge valve, the manual discharge valve is arranged on the side wall of the kettle body, and the filter screen is located between the highest point and the lowest point of the inlet end of the manual discharge valve.

[0020] It further includes,

[0021] a stirring shaft, the stirring shaft is rotatably arranged in the kettle body;

[0022] a rotation driving mechanism, the rotation driving mechanism is arranged outside the kettle body, and the rotation driving mechanism is connected to the stirring shaft;

[0023] a protective cover, and the rotation driving mechanism is arranged inside the protective cover.

[0024] The working principle and beneficial effects of the present utility model are as follows: The bottom of the kettle body has an opening; the chassis is hermetically arranged at the opening at the bottom of the kettle body, the upper end surface of the chassis is a conical surface that is concave downward in the middle, and the chassis has a filtrate outlet, and the filtrate outlet is located at the lowest point of the conical surface of the chassis. During the reaction, the filtrate outlet is closed, and when discharging is required, the filtrate outlet is opened. The upper surface of the chassis is low in the middle and high around, which is convenient for completely discharging the filtrate. There is no need for an additional gas source to supply the opening and closing of the filtrate outlet, no additional gas circuit needs to be added on site, and the chassis does not need to be disassembled for discharging. The structure is simple, time-saving and labor-saving. Brief Description of the Drawings

[0025] The following will further illustrate the above-mentioned features, technical features, advantages and their implementation manners of the present utility model in a clear and understandable manner in combination with the drawings of the preferred embodiments.

[0026] Figure 1 It is a schematic diagram of the internal structure of the present utility model.

[0027] Figure 2 It is a schematic diagram of the connection structure between the chassis and the kettle body of the present utility model.

[0028] Figure 3 It is a schematic diagram of the bottom structure of the present utility model.

[0029] Figure 4 It is a schematic diagram of the structure of the present utility model in the first direction.

[0030] Figure 5 It is a schematic diagram of the structure of the present utility model in the second direction.

[0031] Figure 6 It is a schematic diagram of the structure of the present utility model in the third direction.

[0032] Figure 7 It is a schematic diagram of the structure of the top of the present utility model in one direction.

[0033] Figure 8 It is a schematic diagram of the structure of the top of the present utility model in another direction.

[0034] Figure 9 It is a schematic diagram of the structure of the present utility model when cooperating with the mobile cart.

[0035] In the figure: 1. Kettle body, 2. Chassis, 3. Filtrate outlet, 4. Three-way valve, 5. Filter screen, 6. Plug valve, 7. Heating chamber, 8. Heating medium input pipe, 9. Heating medium output pipe, 10. Temperature sensor, 11. Insulation chamber, 12. Strip-shaped sight glass, 13. Manhole sight glass, 14. Pressure sensor interface, 15. Rupture disc interface, 16. Manual discharge valve, 17. Stirring shaft, 18. Rotary drive mechanism, 19. Protective cover, 20. Reducer, 21. First leg, 22. Second leg, 23. Mobile cart, 24. Bearing plate. Detailed Embodiments

[0036] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will describe the specific embodiments of the present invention with reference to the accompanying drawings. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings, and other embodiments can also be obtained.

[0037] To make the drawings concise, only the parts related to the utility model are schematically shown in each drawing, and they do not represent the actual structure of the product. In addition, to make the drawings concise and easy to understand, in some drawings, components with the same structure or function are only schematically shown for one of them, or only one of them is marked. In this article, "one" not only means "only this one", but also can mean "more than one" situation, and "several" includes "two" and "more than two".

[0038] In this article, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0039] In addition, in the description of this application, the terms "first", "second", etc. are only used for distinguishing descriptions, and cannot be understood as indicating or implying relative importance.

[0040] Example, referring to Figure 1 、 Figure 2 、 Figure 3 and Figure 4 , which is an embodiment of the present invention, provides a polypeptide synthesis reactor with convenient operation and multiple functions, including a reactor body 1, a chassis 2, and the bottom of the reactor body 1 has an opening; the chassis 2 is hermetically arranged at the opening at the bottom of the reactor body 1, the upper end surface of the chassis 2 is a conical surface that is concave downward in the middle, the chassis 2 has a filtrate outlet 3, and the filtrate outlet 3 is located at the lowest point of the conical surface of the chassis 2. When reacting, the filtrate outlet 3 is closed, and when discharging, the filtrate outlet 3 is opened. The upper surface of the chassis 2 is low in the middle and high around, which is convenient for completely discharging the filtrate. There is no need for an additional gas source to supply the opening and closing of the filtrate outlet 3, and there is no need to additionally increase the gas path on site, nor to disassemble the chassis 2 to discharge the material. The structure is simple, time-saving and labor-saving. The chassis 2 is connected to the flange at the lower end of the reactor body 1 through a C-shaped clamp, which is simple, fast and convenient for installation and disassembly.

[0041] Furthermore, as Figure 1 and Figure 2As shown, it further includes a three-way valve 4. The three-way valve 4 is arranged under the chassis 2. The three-way valve 4 has an inert gas inlet, a filtrate discharge port, and a communication port. The communication port of the three-way valve 4 is communicated with the filtrate outlet 3. When discharging the filtrate, the inert gas inlet of the three-way valve 4 is closed. The filtrate in the kettle body 1 directly enters the inside of the three-way valve 4 through the filtrate outlet 3 and the communication port of the three-way valve 4, and then is discharged through the filtrate discharge port of the three-way valve 4. During normal reaction synthesis, the filtrate discharge port of the three-way valve 4 is closed, and the inert gas inlet of the three-way valve 4 is opened. The inert gas is sent into the synthesis reaction kettle through the three-way valve 4, and the inert gas is blown at the bottom of the synthesis reaction kettle through the guiding groove on the chassis 2, so that the solvent and the medium inside the synthesis reaction kettle are effectively separated, the reaction time of each medium is increased, the reaction rate of each synthesis is increased, and the finished product rate after multiple reactions as a whole is improved.

[0042] Furthermore, as Figure 1 and Figure 2 shown, it further includes a filter screen 5 and a plunger valve 6. The filter screen 5 is arranged on the chassis 2 and above the conical surface of the chassis 2; the plunger valve 6 is arranged on the kettle body 1 and outside the kettle body 1. The inlet end of the plunger valve 6 is communicated with the inside of the kettle body 1. The filter screen 5 is located between the highest point and the lowest point of the inlet end of the plunger valve 6. The plunger valve 6 is simultaneously communicated with the cavity above the filter screen 5 and the cavity below the filter screen 5. After the overall reaction synthesis is completed, the plunger valve 6 is opened, so that the medium and the final washing liquid are discharged into a designated container in the form of a liquid mixture through the plunger valve 6. There is no need to disassemble the chassis 2, which can avoid the medium being exposed to the outside, can also avoid its odor polluting the surrounding area, and can also avoid the medium being polluted by the outside. The filter screen 5 is integrally sintered by a stainless steel multi-layer woven mesh and a perforated plate. The filter screen 5 is connected to the chassis 2 through bolts at the bottom. When replacing, as long as the bolts are removed, the filter screen 5 can be easily taken out and replaced, which is convenient and fast. The function of the filter screen 5 is to filter the solvent in different links during the reaction process, and the filtered filtrate is concentrated in the chassis 2.

[0043] Furthermore, as Figure 1 and Figure 2As shown in the figure, the chassis 2 has a heating chamber 7, which is located below the conical surface of the chassis 2. It also includes a heating medium input pipe 8, a heating medium output pipe 9, and a temperature sensor 10. One end of the heating medium input pipe 8 is located outside the chassis 2, and the other end of the heating medium input pipe 8 communicates with the heating chamber 7. One end of the heating medium output pipe 9 communicates with the heating chamber 7, and the other end of the heating medium output pipe 9 is located outside the chassis 2. The temperature sensor 10 is arranged on the kettle body 1. The temperature sensor 10 has a detection end and a display end. The detection end of the temperature sensor 10 is located inside the kettle body 1, and the display end of the temperature sensor 10 is located outside the kettle body 1. The heating medium enters the heating chamber 7 through the heating medium input pipe 8 and is discharged through the heating medium output pipe 9. The heating medium in the heating chamber 7 can increase the heat exchange area of the entire synthesis reactor, greatly shortening the time for temperature adjustment inside the kettle body 1. The real-time temperature can be displayed through the temperature sensor 10 on the kettle body 1, facilitating the control of the temperature inside the kettle body 1 at any time.

[0044] Further, as Figure 1 and Figure 2 shown, the chassis 2 has a heat preservation chamber 11, which is located below the heating chamber 7. The heat preservation chamber 11 is filled with heat preservation materials, which can effectively reduce the heat dissipation inside the heating chamber 7, maintain the temperature stability inside the reactor, save energy, and reduce the operating cost.

[0045] Further, as Figure 6 shown, it also includes a strip-shaped sight glass 12, which is arranged on the side wall of the kettle body 1. The strip-shaped sight glass 12 can provide a long strip-shaped observation window, allowing the operator to monitor the inert gas bubbling state and the liquid rotation state during stirring inside the kettle body 1 in real time without opening the kettle body 1. The operator will not come into contact with the materials inside the kettle body 1, which can improve the operation safety, avoid the reactants being affected by the external environment, and can also reduce the loss of materials and energy. Arranging the strip-shaped sight glass 12 on the side wall of the kettle body 1 saves more space.

[0046] Further, as Figure 6 、 Figure 7 and Figure 8 shown, it also includes a manhole sight glass 13, which is arranged on the top of the kettle body 1. The manhole sight glass 13 allows the operator to directly observe the state, color, liquid level, etc. of the reactants inside the kettle body 1 without opening the kettle body 1. The operator will not come into contact with the materials inside the kettle body 1, which can improve the operation safety, avoid the reactants being affected by the external environment, and can also reduce the loss of materials and energy. Arranging the manhole sight glass 13 on the top of the kettle body 1 can save floor space and make the layout of the synthesis reactor more flexible.

[0047] Further, as Figure 7 and Figure 8As shown in the figure, a pressure sensor interface 14 and a rupture disc interface 15 are provided at the top of the kettle body 1. After installing a pressure sensor on the pressure sensor interface 14, the pressure inside the kettle body 1 can be monitored in real time. When the pressure inside the kettle body 1 exceeds the preset safety limit, the pressure sensor can trigger the alarm system to notify the operator to take measures in time to avoid overpressure accidents, making it safer and more reliable. After installing a rupture disc on the rupture disc interface 15, when the pressure inside the kettle body 1 exceeds the preset rupture pressure of the rupture disc, the rupture disc will be damaged and the pressure will be quickly released to prevent the reaction kettle from being damaged or exploded due to overpressure.

[0048] Furthermore, as Figure 3 , Figure 5 and Figure 6 shown, it also includes a manual discharge valve 16. The manual discharge valve 16 is arranged on the side wall of the kettle body 1, and the filter screen 5 is located between the highest point and the lowest point of the inlet end of the manual discharge valve 16. The manual discharge valve 16 is an extended structure. In special cases, when it is inconvenient to use the plunger valve 6 to pump materials, a glove box can be configured outside the manual discharge valve 16. Through the self-filtration function of the synthesis reaction kettle, after the cleaned medium is filtered in the synthesis reaction kettle, the manual discharge valve 16 is opened through the glove box to manually take out the medium material without disassembling the chassis 2. The whole process is also carried out in a closed environment, isolating the medium material from people and air, which can avoid the odor pollution of the medium material to the outside world, and the outside world cannot pollute the medium material either.

[0049] Furthermore, as Figure 1 shown, it also includes a stirring shaft 17, a rotary drive mechanism 18, and a protective cover 19. The stirring shaft 17 is rotatably arranged inside the kettle body 1; the rotary drive mechanism 18 is arranged outside the kettle body 1, and the rotary drive mechanism 18 is connected to the stirring shaft 17; the rotary drive mechanism 18 is arranged inside the protective cover 19. The rotary drive mechanism 18 is used to drive the stirring shaft 17 to rotate and stir the materials inside the kettle body 1. By arranging the rotary drive mechanism 18 inside the protective cover 19, it can be effectively protected without affecting the heat dissipation of the rotary drive mechanism 18, which can avoid the situation of difficult cleaning of the equipment hygiene dead corners caused by its external placement and can increase the appearance beauty of the equipment. The rotary drive mechanism 18 is preferably an explosion-proof motor. It also includes a speed reducer 20. The output shaft of the rotary drive mechanism 18 is connected to the input shaft of the speed reducer 20, and the output shaft of the speed reducer 20 is connected to the upper end of the stirring shaft 17. The speed reducer 20 can be used to adjust the rotation speed of the stirring shaft 17. The speed reducer 20 is also arranged inside the protective cover 19. It can be effectively protected without affecting the heat dissipation of the speed reducer 20, which can avoid the situation of difficult cleaning of the equipment hygiene dead corners caused by its external placement and can increase the appearance beauty of the equipment. Stirring paddles are arranged on the stirring shaft 17. Through the rotation of the stirring paddles, the solvent and the medium inside the synthesis reaction kettle can be effectively separated.

[0050] Further, as Figure 9 shown, it further includes a first leg 21 and a second leg 22. The upper end of the first leg 21 is connected to the kettle body 1, and the lower end of the first leg 21 is located below the kettle body 1. The number of the first legs 21 is at least two, and all the first legs 21 are arranged around the kettle body 1 evenly; the upper end of the second leg 22 is arranged on the chassis 2, and the lower end of the second leg 22 is located above the lower end of the first leg 21. The number of the second legs 22 is at least three, and all the second legs 22 are arranged around the chassis 2 evenly. The width of the moving vehicle 23 is smaller than the distance between two adjacent first legs 21; the bearing plate 24 is arranged on the moving vehicle 23 in a lifting manner for bearing the second legs 22. The number of the second legs 22 is preferably four and is arranged in a matrix. When the chassis 2 needs to be disassembled, first move the moving vehicle 23 from between two adjacent first legs 21 to below the second legs 22, then raise the bearing plate 24. After the second legs 22 contact the bearing plate 24, loosen the C-type clamp connecting the flange at the lower end of the kettle body 1 and the chassis 2, and let the chassis 2 fall onto the bearing plate 24 of the moving vehicle 23. After moving the moving vehicle 23 to the outside, the filter screen 5 can be disassembled, cleaned or replaced conveniently.

[0051] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present invention, and they should all be covered by the scope of the claims of the present invention.

Claims

1. A convenient and multifunctional polypeptide synthesis reactor, characterized in that: include, A kettle body (1), wherein the bottom of the kettle body (1) has an opening; A bottom plate (2), the bottom plate (2) being sealingly arranged at the opening at the bottom of the kettle body (1), the upper end surface of the bottom plate (2) being a conical surface with a downward depression in the middle, the bottom plate (2) having a filtrate outlet (3), the filtrate outlet (3) being located at the lowest point of the conical surface of the bottom plate (2); It also comprises a three-way valve (4), the three-way valve (4) being arranged below the bottom plate (2), the three-way valve (4) having an inert gas inlet, a filtrate discharge port and a communication port, the communication port of the three-way valve (4) being in communication with the filtrate outlet (3); Also includes, A filter screen (5), the filter screen (5) being arranged on the bottom plate (2) and located above the conical surface of the bottom plate (2); A plunger valve (6), the plunger valve (6) being arranged on the kettle body (1) and located outside the kettle body (1), the inlet end of the plunger valve (6) being connected to the interior of the kettle body (1), and the filter screen (5) being located between the highest point and the lowest point of the inlet end of the plunger valve (6).

2. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: The bottom plate (2) has a heating chamber (7), the heating chamber (7) is located below the conical surface of the bottom plate (2), and further comprises: a heating medium input pipe (8), one end of the heating medium input pipe (8) being located outside the base plate (2), and the other end of the heating medium input pipe (8) being connected to the heating chamber (7); a heating medium output pipe (9), one end of the heating medium output pipe (9) being in communication with the heating chamber (7), and the other end of the heating medium output pipe (9) being located outside the chassis (2); A temperature sensor (10), the temperature sensor (10) being arranged on the kettle body (1), the temperature sensor (10) comprising a detection end and a display end, the detection end of the temperature sensor (10) being located inside the kettle body (1), and the display end of the temperature sensor (10) being located outside the kettle body (1).

3. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 2, characterized in that: The bottom plate (2) has a heat preservation cavity (11), and the heat preservation cavity (11) is located below the heating cavity (7).

4. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: It also comprises a strip-shaped sight glass (12), wherein the strip-shaped sight glass (12) is arranged on the side wall of the kettle body (1).

5. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: It also comprises a hand hole sight glass (13), wherein the hand hole sight glass (13) is arranged on the top of the kettle body (1).

6. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: A pressure sensor interface (14) and a bursting disc interface (15) are provided on the top of the kettle body (1).

7. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: It also comprises a manual discharge valve (16), the manual discharge valve (16) being arranged on the side wall of the kettle body (1), and the filter screen (5) being located between the highest point and the lowest point of the inlet end of the manual discharge valve (16).

8. The easy-to-operate and multifunctional polypeptide synthesis reactor according to claim 1, characterized in that: Also includes, a stirring shaft (17), the stirring shaft (17) being rotatably disposed in the kettle body (1); a rotary drive mechanism (18), the rotary drive mechanism (18) being arranged outside the kettle body (1), the rotary drive mechanism (18) being connected to the stirring shaft (17); A protective cover (19), wherein the rotary drive mechanism (18) is arranged inside the protective cover (19).

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