Thermal cover assembly of gene amplification instrument
By designing multiple independent seals and a heat cap assembly of movable press rods in the gene amplification device, the problem of difficult sealing the hot cap plane in the prior art is solved, and effective sealing of the sample tube is achieved to prevent reagents from evaporating or escaping, ensuring the accuracy of the detection results.
Patent Information
- Application Number
- CN202420694091.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-07
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-04-07
AI Technical Summary
The thermal cover of the existing PCR instrument is planar, making it difficult to achieve effective sealing of the sample tube, resulting in evaporation or escape of the reagent, affecting the accuracy of the detection results.
A thermal cap assembly of the gene amplification instrument is designed, and multiple independent seals are used to contact the sample tube. Through the deformation of the seal and the movement of the pressure rod, the sample tube is sealed to ensure that the reagent does not evaporate or escape.
Effectively prevent the evaporation or escape of reagents in the sample tube, improve the sealing effect, ensure the accuracy of biochemical test results, and even if a seal fails, it is easy to replace and reduce maintenance costs.
Smart Images
Figure CN223047481U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of gene detection, and particularly relates to a thermal cover assembly of a gene amplifier. Background Art
[0002] PCR (Polymerase Chain Reaction), namely polymerase chain reaction, is a process of using a segment of DNA as a template and, with the participation of DNA polymerase and nucleotide substrates, amplifying this segment of DNA to a sufficient quantity for structural and functional analysis.
[0003] During the PCR amplification reaction process, there are thermal reactions with relatively high temperatures, long reaction times, or rapid temperature changes. Due to the rapid temperature changes during PCR gene amplification and the large temperature difference with the ambient temperature, the reagent inevitably has a serious evaporation phenomenon, thereby generating aerosol. If the aerosol overflows, it will cause contamination problems and affect the normal progress of the reaction, as well as the accuracy of biochemical test results. Therefore, existing PCR instruments are all equipped with a thermal cover (a metal plate basically in a planar shape). Since the thermal cover is heated and its temperature is higher than that of the reagent, based on the principle of cold air flowing downward, it can not only prevent the overflow of aerosol but also prevent the reagent from condensing on the upper thermal cover, thus ensuring the accuracy of biochemical test results.
[0004] However, since the thermal cover itself is a planar product, it is difficult to achieve the sealing of the sample tube simply through the planar contact between the thermal cover and the sample tube, and there are still problems of reagent evaporation or escape. Summary of the Utility Model
[0005] To solve the above technical problems, the utility model provides a thermal cover assembly of a gene amplifier and a gene amplifier, which seal the sample tubes through multiple independent seals, and have a good sealing effect.
[0006] The utility model adopts the following technical solutions:
[0007] A thermal cover assembly of a gene amplifier includes: a thermal cover bracket; a thermal cover installed on the thermal cover bracket; and a plurality of seals independently arranged on the thermal cover, used to correspond to each sample tube of a multi-well plate placed on the gene amplifier body one by one to achieve sealing. By using multiple independent seals to individually seal each sample tube, the sealing effect is good, and it can effectively prevent the evaporation or escape of the reagent in the sample tube.
[0008] Preferably, the seal is made of rubber or silica gel, and its Shore A hardness is 10 - 100. When sealed on the sample tube, it can produce a certain deformation and fit on the opening of the sample tube to better seal the sample tube.
[0009] Preferably, a ring-shaped protrusion is formed at the open end of the sample tube, and a ring-shaped groove is provided inside the seal corresponding to the position of the ring-shaped protrusion, so that the seal parts on both sides of the ring-shaped groove are extruded toward the concave part, realizing the effect of fully wrapping the open end of the sample tube and improving the sealing effect.
[0010] Preferably, the thermal cover assembly further includes a plurality of pressure rods mounted on the thermal cover, and the seal is provided at the end of each pressure rod facing the sample tube; the seal and the pressure rod can move relative to the thermal cover along the axial direction of the pressure rod, and the product has better adaptability: even if the surface of the multi-well plate is uneven, or the surface of the thermal cover is uneven, or there are mechanical errors or assembly errors, each seal can form a reliable seal with each sample tube adaptively.
[0011] Preferably, the seal and the pressure rod can displace relative to the thermal cover along the first direction after being acted on by the sample tube, and can displace relative to the thermal cover along the second direction after separating from the contact with the sample tube; the first direction and the second direction are opposite.
[0012] Preferably, the thermal cover assembly further includes: a positioning plate provided on the thermal cover; a plurality of pressure rods, each of which is provided with the seal at its end facing the sample tube, penetrates through the thermal cover, and can move relative to the thermal cover and the positioning plate along its axial direction; a plurality of return springs sleeved outside the pressure rods, one end abuts against the positioning plate, and the other end abuts against the pressure rod, and the seal can be tightly pressed on the sample tube through the return springs to achieve sealing.
[0013] Preferably, the end of the pressure rod away from the seal passes through the positioning plate, and a limit ring for preventing the pressure rod from falling off the positioning plate is provided, and the pressure rod is prevented from detaching from the positioning plate through the limit ring to realize the limit of the pressure rod.
[0014] Preferably, the pressure rod includes a pressure rod body, a limit portion formed on the pressure rod body for preventing the seal from falling off, and a convex portion formed at the end of the pressure rod body; the seal is coated on the limit portion and the convex portion.
[0015] Preferably, a ring-shaped groove is provided at the end of the seal away from the sample tube and corresponding to the position of the ring-shaped protrusion of the sample tube, the ring-shaped groove is located outside the convex portion, and its open end abuts against the limit portion.
[0016] Preferably, the end of the convex portion is exposed outside the seal, which is convenient for heat conduction and improves the heat efficiency.
[0017] Preferably, a plurality of guiding holes are formed in the heat cover bracket, and a plurality of guiding columns are formed in the heat cover and penetrate through the guiding holes coaxially. A compression spring is sleeved outside the guiding columns, and two ends of the compression spring respectively abut against the heat cover bracket and the heat cover. In this way, the heat cover assembly has a larger compensation range.
[0018] Preferably, the heat cover assembly further includes: a heat cover housing; a handle rotatably mounted on the heat cover housing through a rotating shaft, and at least two arc-shaped guiding grooves are provided thereon; a pin shaft mounted on the heat cover bracket, and two ends of the pin shaft are respectively embedded in the two arc-shaped guiding grooves; by rotating the handle, the heat cover bracket, the heat cover and the seal can move relative to the heat cover housing between a first position and a second position.
[0019] Preferably, at the second position, the seal seals each sample tube of the porous plate one by one; at the first position, the seal disengages from the sealed contact with each sample tube of the porous plate.
[0020] Preferably, a locking hook is further provided on the handle for cooperating with a locking post located on the heat cover housing to realize the locking and positioning of the heat cover assembly of the gene amplifier and the gene amplifier body.
[0021] Preferably, at the second position, the locking hook cooperates with the locking post to lock; at the first position, the locking hook is separated from the locking post.
[0022] Compared with the prior art, the utility model has the following advantages:
[0023] 1. The utility model provides a heat cover assembly of a gene amplifier, which includes a heat cover bracket, a heat cover and a plurality of seals. Each sample tube is separately sealed by a plurality of independent seals. Compared with the prior art that uses a flat heat cover for sealing, the sealing effect is good, and it can effectively prevent the evaporation or escape of the reagent in the sample tube. In addition, even if a certain seal fails due to aging or other reasons, resulting in poor sealing effect, the seal can be directly replaced, and the overall maintenance cost is low.
[0024] 2. Since each pressure rod and the seal mounted on the pressure rod can move relative to the heat cover along the axial direction of the pressure rod, the product has better adaptability: even if the surface of the porous plate is uneven, or the surface of the heat cover is uneven, or there are mechanical errors or assembly errors, each seal can adaptively form a reliable seal with each sample tube.
[0025] 3. An annular protrusion is formed at the open end of the sample tube. An annular groove is provided on the sealing member at a position away from the sample tube and corresponding to the annular protrusion. After the sealing member is squeezed by the annular protrusion of the sample tube, the parts on both sides of the annular groove will turn outwards and deform to a certain extent. With the squeezing effect given by the annular protrusion of the sample tube from the bottom, the sealing member can give a squeeze to the annular protrusion of the sample tube from the side after deformation, so that the sealing member can fully wrap the open end of the sample tube, improving the sealing effect.
[0026] 4. For the present utility model, only by turning the handle downwards can the locking be achieved. At the same time, the heat cover bracket, the heat cover and the sealing member move downwards, and each sample tube is separately sealed by a plurality of independent sealing members, with good sealing effect. Description of the Drawings
[0027] Figure 1 It is a schematic diagram of the use state of the heat cover assembly.
[0028] Figure 2 It is a sectional view of the heat cover assembly.
[0029] Figure 3 It is a schematic diagram of the structure in the state where the sealing member is separated from the contact with the sample tube.
[0030] Figure 4 It is a schematic diagram of the structure in the state where the sealing member seals the sample tube.
[0031] Figure 5 For Figure 4 Partial enlarged view.
[0032] Figure 6 It is a partial sectional view of the heat cover assembly.
[0033] Figure 7 For Figure 6 Partial enlarged view of part C of
[0034] Figure 8 It is a schematic diagram of the open state of the heat cover assembly.
[0035] Figure 9 It is an assembly diagram of the heat cover and the positioning plate.
[0036] In the figure, heat cover bracket 1, guide hole 1-1, heat cover 2, guide post 2-1, sealing member 3, annular groove 3-1, pressure rod 4, pressure rod body 4-1, limiting part 4-2, convex part 4-3, positioning plate 5, return spring 6, limiting ring 7, compression spring 8, heat cover housing 9, handle 10, arc-shaped guide groove 10-1, locking hook 10-2, pin shaft 11, locking column 12, heat cover lower shell 13. Detailed Description of the Invention
[0037] To facilitate the understanding of the technical solution of the present utility model, the following provides a detailed description in conjunction with the accompanying drawings and specific embodiments.
[0038] As Figures 1-4 shown, a thermal cover assembly of a gene amplifier includes:
[0039] A thermal cover bracket 1;
[0040] A thermal cover 2, mounted on the thermal cover bracket 1;
[0041] A plurality of seals 3, independently arranged on the thermal cover 2, for sealingly corresponding to each sample tube of the multi-well plate placed on the gene amplifier body one by one. In this embodiment, the seal 3 is made of rubber or silica gel, and its Shore A hardness is 10-100. When the seal 3 is pressed against the sample tube, it can produce a certain deformation, fit on the opening of the sample tube, and preferably seal the sample tube. At the same time, its Shore A hardness is 10-100, not too soft (if too soft, the interaction force between the seal and the sample tube is small, resulting in poor sealing effect), nor too hard (if too hard, the deformation of the seal is small, resulting in poor sealing effect). Since the seal 3 itself has a certain elastic deformation ability, even if the surface of the multi-well plate or the thermal cover is uneven, each seal 3 can adaptively form a reliable seal with each sample tube.
[0042] In some practical applications, the thermal cover assembly further includes a plurality of pressure rods 4 mounted on the thermal cover 2, and each pressure rod 4 is provided with the seal 3 at the end facing the sample tube; the seal 3 and the pressure rod 4 can move relative to the thermal cover 2 along the axial direction of the pressure rod 4. Thus, since each pressure rod 4 and the seal 3 mounted on the pressure rod 4 can move relative to the thermal cover 2 along the axial direction of the pressure rod 4, the product has better adaptability: even if the surface of the multi-well plate is uneven, or the surface of the thermal cover is uneven, or there are mechanical errors or assembly errors, each seal 3 can adaptively form a reliable seal with each sample tube. In practical applications, the seal 3 and the pressure rod 4 move relative to the thermal cover 2 along the axial direction of the pressure rod 4, and the specific implementation method can be realized by a motor driving a gear-rack structure, or by a spring (the spring compresses and resets to achieve reciprocating motion). In some other practical applications, the seal 3 and the pressure rod 4 can displace relative to the thermal cover 2 along a first direction after being acted on by the sample tube, and can displace relative to the thermal cover 2 along a second direction after separating from the contact with the sample tube; the first direction and the second direction are opposite. Specifically, the second direction is the direction in which the seal 3 faces the sample tube.
[0043] As Figure 6 、 Figure 7 shown, in some other practical applications, the thermal cover assembly further includes:
[0044] The positioning plate 5 is arranged on the heat cover 2;
[0045] A plurality of pressing rods 4 are provided with the sealing members 3 at their ends facing the sample tube, penetrate through the heat cover 2, and can move axially relative to the heat cover 2 and the positioning plate 5;
[0046] A plurality of return springs 6 are sleeved outside the pressing rods 4 one by one, with one end abutted against the positioning plate 5 and the other end abutted against the pressing rods 4.
[0047] The heat cover 2 has a plurality of through holes matching the pressing rods 4, and the pressing rods 4 can be slidably fitted on the heat cover 2 and move axially relative to the heat cover 2 and the positioning plate 5 in the through holes. After the heat cover assembly is closed, on the one hand, the sealing member 3 will be deformed by the action of the sample tube, so as to achieve sealed contact with the sample tube. On the other hand, the return spring 6 will also be compressed (which means the pressing rod 4 moves upward relative to the heat cover 2). Under the action of the return spring 6, the sealing member 3 is tightly pressed on the sample tube to achieve better sealing. For the convenience of understanding, the following examples are given for illustration, but it is not regarded as a limitation to the present utility model. For example, during the process of closing the heat cover assembly, the sealing member 3 and the pressing rod 4 move downward with the heat cover assembly. After the sealing member 3 contacts the sample tube, it continues to move downward, and the sealing member 3 will be deformed by the action of the sample tube; continue to close the heat cover assembly, the sealing member 3 and the pressing rod 4 continue to move downward with the heat cover assembly, and the sealing member 3 will be further deformed until it no longer deforms; continue to close the heat cover assembly, the sealing member 3 and the pressing rod 4 no longer continue to move downward, but move upward relative to the heat cover 2. At this time, the return spring 6 is compressed and in a compressed state, keeping the relative positions of the sealing member 3, the pressing rod 4 and the sample tube unchanged. At the same time, under the action of the return spring 6, the sealing member 3 is tightly pressed on the sample tube to achieve better sealing. During the process of opening the heat cover assembly, it is just the opposite. The sealing member 3 and the pressing rod 4 can move downward relative to the heat cover 2 under the action of the return spring 6 after separating from the contact with the sample tube, or can move downward relative to the heat cover 2 under the action of the return spring 6 in the state of contacting the sample tube.
[0048] As Figure 9 shown, the positioning plate 5 is provided with a plurality of through holes matching the pressing rods 4. The end of the pressing rod 4 far from the sealing member 3 passes through the through hole of the positioning plate 5, and a limiting ring 7 is provided to prevent the pressing rod 4 from falling off the positioning plate 5. The pressing rod 4 is slidably fitted in the through hole of the positioning plate 5, and the pressing rod 4 is prevented from detaching from the positioning plate 5 by the limiting ring 7, so as to realize the limitation of the pressing rod 4.
[0049] As Figure 3 shown, in some practical applications, a ring-shaped protrusion is formed at the open end of the sample tube, and a ring-shaped groove 3-1 is formed inside the sealing member 3 corresponding to the ring-shaped protrusion, that is, the upper end in the illustrated direction has the ring-shaped groove 3-1. As Figure 4As shown in the figure, when the seal 3 is pressed against the sample tube, the lower surface of the seal 3 abuts against the annular protrusion of the sample tube. Under the pressing action of the annular protrusion, the lower surface of the seal 3 is forced to be recessed upward to form a recess for accommodating the annular protrusion, and the elastic deformation of the seal 3 is used to achieve its sealed contact with the sample tube. At the same time, due to the existence of the annular groove 3-1, after the seal 3 is squeezed by the annular protrusion of the sample tube, the parts on both sides of the annular groove 3-1 will turn outward to a certain extent. Cooperating with the squeezing action given by the annular protrusion of the sample tube from the bottom, the seal 3 can give an extrusion to the annular protrusion of the sample tube from the side after deformation (such as Figure 5 the arrow direction shown), so that the seal 3 can achieve the effect of fully wrapping the opening end of the sample tube and improve the sealing effect.
[0050] Such as Figure 3 , Figure 4 shown, the pressing rod 4 includes a pressing rod body 4-1, a limiting portion 4-2 formed on the pressing rod body 4-1 for preventing the seal 3 from falling off, and a convex portion 4-3 formed at the end of the pressing rod body 4-1. The seal 3 is coated on the limiting portion 4-2 and the convex portion 4-3 to prevent the seal 3 from falling off the pressing rod 4. In practical applications, it is necessary to heat the reagent in the sample tube to 90-95 degrees Celsius. In order to avoid the evaporation or escape of the reagent, it is necessary to ensure that the temperature at the opening of the sample tube (at the pressing rod and the seal) reaches 105-115 degrees Celsius. Therefore, in order to reduce energy consumption and improve thermal efficiency, the preferred solution of this embodiment is: the middle of the seal 3 is penetrated, the pressing rod 4 is made of metal, and the end of its convex portion 4-3 protrudes from the middle of the seal 3, which is convenient for heat conduction and reduces the temperature requirement for the heating plate on the thermal cover 2 (if the seal completely wraps the end of the pressing rod, due to the poor thermal conductivity of the seal, a higher temperature is required to ensure that the temperature at the opening end of the sample tube remains at the temperature required for PCR amplification). In this structure, in order to avoid the evaporation or escape of the reagent from the gap between the seal 3 and the pressing rod 4, in this embodiment, the annular groove 3-1 of the seal 3 is located outside the convex portion 4-3, and its open end abuts against the limiting portion 4-2. Such as Figure 4As shown, when the seal 3 is pressed against the sample tube, the lower surface of the seal 3 abuts against the annular protrusion of the sample tube. Under the pressing action of the annular protrusion, the lower surface of the seal 3 is forced to form a recessed portion that accommodates the annular protrusion by being depressed upward. The elastic deformation of the seal 3 is utilized to achieve its sealed contact with the sample tube. At the same time, due to the existence of the annular groove 3-1, after the seal 3 is squeezed by the annular protrusion of the sample tube, the portions on both sides of the annular groove 3-1 will undergo a certain amount of outward turning deformation. Among them, the portion of the seal 3 located inside the annular groove 3-1 will deform towards the side wall of the convex portion 4-3 and squeeze the side wall of the convex portion 4-3, thereby improving the sealing effect between the seal 3 and the pressure rod 4. Of course, within the scope optional for those skilled in the art, the convex portion 4-3 can also be completely wrapped by the seal 3 and not exposed outside.
[0051] A number of guiding holes 1-1 are formed on the heat cover bracket 1, and a number of guiding columns 2-1 that penetrate through the guiding holes 1-1 coaxially are formed on the heat cover 2. A compression spring 8 is sleeved outside the guiding column 2-1, and both ends of the compression spring abut against the heat cover bracket 1 and the heat cover 2 respectively. In this way, the heat cover assembly has a greater compensation capacity for the range.
[0052] As Figures 6-8 shown, in some practical applications, the heat cover assembly further includes:
[0053] A heat cover housing 9; in some practical applications, the heat cover housing 9 is made of plastic material;
[0054] A handle 10, which is rotatably installed on the heat cover housing 9 through a rotating shaft, and at least two arc-shaped guiding grooves 10-1 are provided thereon;
[0055] A pin shaft 11, which is installed on the heat cover bracket 1, and both ends of it are respectively embedded in two arc-shaped guiding grooves 10-1; by rotating the handle 10, the heat cover bracket 1, the heat cover 2 and the seal 3 can move relative to the heat cover housing 9 between a first position and a second position. At the second position, the seal 3 seals each sample tube of the porous plate one by one; at the first position, the seal 3 is disengaged from the sealed contact with each sample tube of the porous plate. Since in the prior art, the heat cover assembly is installed on the gene amplification instrument body by means of a hinge, when the heat cover assembly is closed, the heat cover assembly and the heat cover are both rotated to press the sample tube, which will cause the sample tubes near the hinge side to contact the heat cover first and receive greater pressure. Over time, the seals near the hinge side will be more likely to fail, reducing the service life of the product; while after adopting the solution of this embodiment, the movement of the heat cover 2 from the first position to the second position is a linear motion, which can ensure that the pressure on the seals 3 on the entire surface is basically the same, extend the service life of the product, and ensure the sealing effect.
[0056] In some practical applications, a trapezoidal pressing block is provided on the pin shaft 11. The trapezoidal pressing block is located above the hot cover bracket 1. The arc-shaped guide groove 10-1 has a first end (point A) and a second end (point B). In the open state of the hot cover assembly, the pin shaft 11 is located at the second end B of the arc-shaped guide groove 10-1, and the hot cover bracket 1, the hot cover 2, and the seal 3 are in the first position. During use, the handle 10 is rotated downward, and the pin shaft 11 moves relative to the arc-shaped guide groove 10-1 (from point B to point A of the arc-shaped guide groove 10-1), and drives the hot cover bracket 1, the hot cover 2, and the seal 3 to move downward under the action of the wall surface of the arc-shaped guide groove 10-1 to seal the sample tube.
[0057] In other practical applications, a locking hook 10-2 is further provided on the handle 10 for cooperating with a locking post 12 located on the hot cover housing 9 of the gene amplifier to realize the locking and positioning of the hot cover assembly of the gene amplifier and the gene amplifier body. A through hole is provided on the lower surface of the hot cover lower shell 13 corresponding to the position of the locking post 12. When the hot cover lower shell 13 is covered on the gene amplifier body, the locking post 12 is located in the through hole. At this time, when the handle 10 is rotated downward, the pin shaft 11 moves relative to the arc-shaped guide groove 10-1 (from point B to point A of the arc-shaped guide groove 10-1), and the locking of the locking hook 10-2 and the locking post 12 can be realized, and the hot cover bracket 1, the hot cover 2, and the seal 3 are in the second position. When the handle 10 is rotated in the reverse direction, the locking hook 10-2 is disengaged from the locking post 12, forcing the pin shaft 11 to move from point A to point B of the arc-shaped guide groove 10-1, and driving the hot cover bracket 1, the hot cover 2, and the seal 3 to move upward, so that the hot cover bracket 1, the hot cover 2, and the seal 3 are restored to the first position.
[0058] In addition, the multi-well plate placed on the gene amplifier body can be a 96-well plate, or a 48-well, 32-well or multi-well plate with other numbers, as long as the hot cover assembly with different numbers of wells is replaced.
[0059] The above is only the preferred embodiment of the present invention. The protection scope of the present invention is subject to the scope defined by the claims. Several improvements and refinements made by those skilled in the art without departing from the spirit and scope of the present invention should also be regarded as the protection scope of the present invention.
Claims
1. A thermal cover assembly for a gene thermal amplifier, characterized in that: include: Heated cover support (1); A heat cover (2) mounted on the heat cover support (1); A plurality of sealing members (3) are independently arranged on the thermal cover (2) and are used to achieve sealing in a one-to-one correspondence with each sample tube of a multi-well plate placed on the body of the gene amplification instrument; The open end of the sample tube is formed with a circle of annular protrusions, and the sealing member (3) is provided with an annular groove (3-1) at a position away from one end of the sample tube and corresponding to the annular protrusion; The thermal cover assembly further comprises a plurality of pressure rods (4) mounted on the thermal cover (2), and the end of each pressure rod (4) facing the sample tube is provided with the sealing member (3); the sealing member (3) and the pressure rod (4) are capable of moving relative to the thermal cover (2) along the axial direction of the pressure rod (4).
2. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The sealing member (3) is made of rubber or silicone and has a Shore A hardness of 10-100.
3. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The sealing member (3) and the pressure rod (4) are capable of being displaced relative to the thermal cover (2) in a first direction after being subjected to a force from the sample tube, and are capable of being displaced relative to the thermal cover (2) in a second direction after being out of contact with the sample tube; the first direction and the second direction are opposite.
4. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The thermal cover assembly further comprises: A positioning plate (5) is arranged on the heat cover (2); A plurality of pressure rods (4), each of which is provided with the sealing member (3) at the end facing the sample tube, and is arranged to penetrate the thermal cover (2), and is capable of moving along its axial direction relative to the thermal cover (2) and the positioning plate (5); A plurality of return springs (6) are sleeved outside the pressure rod (4), one end of which abuts against the positioning plate (5) and the other end of which abuts against the pressure rod (4).
5. The thermal cover assembly of the gene thermal cycler according to claim 4, characterized in that: One end of the pressure rod (4) away from the sealing member (3) passes through the positioning plate (5), and is provided with a limiting ring (7) for preventing the pressure rod (4) from falling off the positioning plate (5).
6. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The pressure rod (4) comprises a pressure rod body (4-1), a limiting portion (4-2) formed on the pressure rod body (4-1) and used to prevent the sealing member (3) from falling off, and a convex portion (4-3) formed at the end of the pressure rod body (4-1); the sealing member (3) is covered on the limiting portion (4-2) and the convex portion (4-3).
7. The thermal cover assembly of the gene thermal cycler according to claim 6, characterized in that: An annular groove (3-1) is provided on the sealing member (3) at a position away from one end of the sample tube and corresponding to the annular protrusion of the sample tube. The annular groove is located on the outer circle of the protrusion (4-3), and its open end abuts against the limiting portion (4-2).
8. The thermal cover assembly of the gene thermal cycler according to claim 7, characterized in that: The end of the protrusion (4-3) is exposed outside the sealing member (3).
9. The thermal cover assembly of the gene thermal cycler according to claim 1, characterized in that: The heat cover bracket (1) is formed with a plurality of guide holes (1-1), the heat cover (2) is formed with a plurality of guide posts (2-1) coaxially penetrating the guide holes (1-1), and the guide posts (2-1) are provided with compression springs (8) on their outer sleeves, with the two ends of the compression spring respectively abutting against the heat cover bracket (1) and the heat cover (2).
10. The thermal cover assembly of a gene thermal cycler according to claim 1, characterized in that: The thermal cover assembly further comprises: Thermal cover housing (9); A handle (10) is rotatably mounted on the heat cover shell (9) via a rotating shaft, and is provided with at least two arc-shaped guide grooves (10-1); A pin shaft (11) is mounted on the heat cover bracket (1), with two ends thereof respectively embedded in two arc-shaped guide grooves (10-1); by rotating the handle (10), the heat cover bracket (1), the heat cover (2) and the sealing member (3) can be moved between a first position and a second position relative to the heat cover housing (9).
11. The thermal cover assembly of the gene thermal cycler according to claim 10, characterized in that: When in the second position, the sealing member (3) seals each sample tube of the multi-well plate one by one; when in the first position, the sealing member (3) breaks away from the sealing contact with each sample tube of the multi-well plate.
12. The thermal cover assembly of the gene thermal cycler according to claim 10, characterized in that: The handle (10) is also provided with a locking hook (10-2) for cooperating with a locking column (12) located on the body of the gene amplification instrument to achieve locking and positioning of the thermal cover assembly of the gene amplification instrument and the body of the gene amplification instrument.
13. The thermal cover assembly of the gene thermal cycler according to claim 12, characterized in that: When in the second position, the locking hook (10-2) and the locking column (12) cooperate to lock; when in the first position, the locking hook (10-2) and the locking column (12) are separated.