Nucleic acid extraction consumable capable of preventing cross contamination
By adding cross-contamination structure and sealing design to the nucleic acid extraction consumables, the cross-contamination problem during stirring and mixing is solved, and the rapid, accurate and safe nucleic acid extraction is achieved.
Patent Information
- Application Number
- CN202421939964.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-12
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-08-12
AI Technical Summary
The existing nucleic acid extraction consumables have cross-contamination problems during the stirring and mixing of different samples, especially when the sample concentration is high and the reagent sensitivity is high.
A nucleic acid extraction consumable is designed to prevent cross-contamination. By adding a cross-contact structure between the extraction tube body and the magnetic rod sleeve, stirring and mixing are carried out in a close-seal state to prevent aerosol air exchange, and maintaining a complete seal between the magnetic rod sleeve and the extraction tube body during assembly line operations to avoid exchange of aerosol air with the outside air.
It effectively prevents cross-contamination, improves the nucleic acid extraction speed, reduces instrument failures, and avoids accidental pouring of mixed liquid, ensuring the accuracy and efficiency of nucleic acid extraction.
Smart Images
Figure CN223047491U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of extraction consumables, and more specifically, to a nucleic acid extraction consumable for preventing cross-contamination. Background Art
[0002] Common extraction consumables on the market, whether in the form of plates, strips, or single units, are all open. When the magnetic rod sleeve is stirred, whether it is rotated or oscillated up and down, it will not only generate aerosols containing nucleic acid molecules in the sample, but also a large amount of air containing nucleic acid molecule aerosols in the tube will exchange with the air outside the tube.
[0003] During the process of stirring and mixing different samples with existing nucleic acid extraction consumables, varying degrees of cross-contamination will occur, especially when the sample concentration is high and the reagent sensitivity is high, which has the most serious impact.
[0004] Therefore, we have made improvements and proposed a nucleic acid extraction consumable for preventing cross-contamination. Summary of the Utility Model
[0005] The purpose of the utility model is to solve the problem that existing nucleic acid extraction consumables will cause varying degrees of cross-contamination during the process of stirring and mixing different samples, especially when the sample concentration is high and the reagent sensitivity is high, which has the most serious impact.
[0006] In order to achieve the above-mentioned invention purpose, the utility model provides the following technical solutions:
[0007] A nucleic acid extraction consumable for preventing cross-contamination is provided to improve the above problems.
[0008] Specifically, this application is as follows:
[0009] It includes an extraction tube body, a connection sleeve is fixedly installed at the top of the extraction tube body, an extraction tube cover assembly is arranged in the inner cavity of the connection sleeve, a magnetic rod sleeve is arranged in the inner cavity of the connection sleeve, a magnetic rod body is fixedly installed at the bottom of the magnetic rod sleeve, and a sealing groove is opened in the inner cavity of the connection sleeve.
[0010] The design of this consumable adds an intersecting structure and performs a stirring and mixing action in a nearly sealed state, which can effectively prevent air exchange during the stirring and mixing process, keep the aerosol air containing nucleic acid molecules inside the tube, avoid cross-contamination. This consumable has a clamping and completely sealed design. After clamping, the magnetic rod sleeve and the extraction tube body are completely sealed and do not separate, which can effectively prevent the mixed liquid from volatilizing outside the tube and at the same time avoid the exchange of aerosol air containing nucleic acid molecules inside the tube and the air outside the tube. In the assembly line operation, when the stirring rod drives the magnetic rod sleeve to complete the stirring work, the stirring rod separates from the magnetic rod sleeve, and the magnetic rod sleeve, the extraction tube body, and the mixed liquid remain at the lysis / binding position to continue lysis / binding. The stirring rod can move to a new magnetic rod sleeve, a new extraction tube body, and a new sample mixed liquid for stirring and mixing. Within the same time, the mixing and stirring work of multiple samples can be carried out, improving the speed of nucleic acid extraction.
[0011] As a preferred embodiment of the nucleic acid extraction consumable for preventing cross-contamination provided by the present utility model, the extraction tube cover assembly includes an extraction tube cover body, the extraction tube cover body is arranged in the inner cavity of the connecting sleeve, a top cover is fixedly installed on the top of the extraction tube cover body, a sealing sleeve is fixedly installed on the bottom of the extraction tube cover body, and a third sealing block is fixedly installed on the surface of the sealing sleeve.
[0012] As a preferred embodiment of the nucleic acid extraction consumable for preventing cross-contamination provided by the present utility model, a sealing ring is arranged on the surface of the extraction tube cover body, and the size of the sealing ring is adapted to the size of the sealing groove.
[0013] As a preferred embodiment of the nucleic acid extraction consumable for preventing cross-contamination provided by the present utility model, a first sealing block is fixedly installed in the inner cavity of the connecting sleeve, and one side of the first sealing block is in contact with the surface of the magnetic rod sleeve.
[0014] As a preferred embodiment of the nucleic acid extraction consumable for preventing cross-contamination provided by the present utility model, a second sealing block is fixedly installed in the inner cavity of the connecting sleeve, and one side of the second sealing block is in contact with the inner cavity of the magnetic rod sleeve.
[0015] As a preferred embodiment of the nucleic acid extraction consumable for preventing cross-contamination provided by the present utility model, the bottom of the extraction tube body is provided with two tangent arcs, and the two tangent arcs can also be set as standard semi-circles or single arcs or cones.
[0016] Compared with the prior art, the beneficial effects of the present utility model are as follows: The design of this consumable increases the intersecting structure and performs the stirring and mixing action in a nearly sealed state, which can effectively prevent air exchange during the stirring and mixing process, keep the aerosol air containing nucleic acid molecules inside the tube, avoid cross-contamination. This consumable has a clamping and completely sealed design. After clamping, the magnetic rod sleeve and the extraction tube body are completely sealed and do not separate, which can effectively prevent the mixed liquid from volatilizing outside the tube, and at the same time avoid the exchange of aerosol air containing nucleic acid molecules inside the tube and the outside air. In the assembly line operation, when the stirring rod drives the magnetic rod sleeve to complete the stirring work, the stirring rod separates from the magnetic rod sleeve, and the magnetic rod sleeve, the extraction tube body, and the mixed liquid remain at the lysis / binding position to continue lysis / binding. The stirring rod can move to a new magnetic rod sleeve, a new extraction tube body, and a new sample mixed liquid for stirring and mixing. In the same time, the mixing and stirring work of multiple samples can be carried out, improving the speed of nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 FIG. is a schematic structural diagram of the nucleic acid extraction consumable for preventing cross-contamination provided by the present application;
[0018] Figure 2 FIG. is a front sectional view of the connecting sleeve of the nucleic acid extraction consumable for preventing cross-contamination provided by the present application;
[0019] Figure 3 FIG. is a front sectional view of the extraction tube of the nucleic acid extraction consumable for preventing cross-contamination provided by the present application;
[0020] Figure 4 FIG. is a front view of the extraction tube cover assembly of the nucleic acid extraction consumable for preventing cross-contamination provided by the present application.
[0021] Reference numerals in the figures:
[0022] 1. Extraction tube body; 2. Connecting sleeve; 3. Extraction tube cover assembly; 301. Extraction tube cover body; 302. Top cover; 303. Sealing sleeve; 304. Third sealing block; 305. Sealing ring; 4. Magnetic rod sleeve; 5. Magnetic rod body; 6. Sealing groove; 7. First sealing block; 8. Second sealing block. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0023] To make the objectives, technical solutions, and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present utility model.
[0024] Accordingly, the following detailed description of the embodiments of the present utility model is not intended to limit the scope of the present utility model claimed, but merely represents some embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the scope of protection of the present utility model.
[0025] It should be noted that, without conflict, the embodiments in the present utility model and the features and technical solutions in the embodiments may be combined with each other.
[0026] It should be noted that like reference numerals and letters denote like items in the following figures. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.
[0027] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by terms such as "upper", "lower", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship when the inventive product is normally placed, or the orientation or positional relationship commonly understood by those skilled in the art. Such terms are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present utility model. In addition, terms such as "first", "second", etc. are only used for descriptive distinction and cannot be understood as indicating or implying relative importance.
[0028] As described in the background art, during the process of stirring and mixing different samples with existing nucleic acid extraction consumables, varying degrees of cross-contamination will occur, especially when the sample concentration is high and the reagent sensitivity is high, which has the most serious impact.
[0029] To solve this technical problem, the present utility model provides a nucleic acid extraction consumable for preventing cross-contamination.
[0030] Specifically, please refer to Figures 1-4 , the nucleic acid extraction consumable for preventing cross-contamination specifically includes: an extraction tube body 1, a connection sleeve 2 is fixedly installed at the top of the extraction tube body 1, an extraction tube cover assembly 3 is arranged in the inner cavity of the connection sleeve 2, a magnetic rod sleeve 4 is arranged in the inner cavity of the connection sleeve 2, a magnetic rod body 5 is fixedly installed at the bottom of the magnetic rod sleeve 4, and a sealing groove 6 is opened in the inner cavity of the connection sleeve 2.
[0031] The design of this consumable adds an intersecting structure and performs a stirring and mixing operation in a nearly sealed state, which can effectively prevent air exchange during the stirring and mixing process, keep the aerosol air containing nucleic acid molecules inside the tube, avoid cross-contamination. This consumable has a clamping and completely sealed design. After clamping, the magnetic rod sleeve 4 and the extraction tube body 1 are completely sealed and do not separate, which can effectively prevent the mixed liquid from volatilizing outside the tube and at the same time avoid the exchange of aerosol air containing nucleic acid molecules inside the tube and the air outside the tube. In the assembly line operation, when the stirring rod drives the magnetic rod sleeve 4 to complete the stirring work, the stirring rod separates from the magnetic rod sleeve 4, and the magnetic rod sleeve 4, the extraction tube body 1, and the mixed liquid remain at the lysis / binding position to continue lysis / binding. The stirring rod can move to a new magnetic rod sleeve 4, a new extraction tube body 1, and a new sample mixed liquid for stirring and mixing. In the same time, the mixing and stirring work of multiple samples can be carried out, improving the speed of nucleic acid extraction.
[0032] In order to enable those skilled in the art of this technology to better understand the solution of this utility model, the technical solutions in the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings.
[0033] It should be noted that, without conflict, the embodiments in this utility model and the features and technical solutions in the embodiments can be combined with each other.
[0034] It should be noted that similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.
[0035] Embodiment 1
[0036] Please refer to Figures 1-4 , a nucleic acid extraction consumable for preventing cross-contamination, including an extraction tube body 1. A connection sleeve 2 is fixedly installed at the top of the extraction tube body 1. An extraction tube cover assembly 3 is arranged in the inner cavity of the connection sleeve 2. A magnetic rod sleeve 4 is arranged in the inner cavity of the connection sleeve 2. A magnetic rod body 5 is fixedly installed at the bottom of the magnetic rod sleeve 4. A sealing groove 6 is opened in the inner cavity of the connection sleeve 2. A first sealing block 7 is fixedly installed in the inner cavity of the connection sleeve 2. One side of the first sealing block 7 is in contact with the surface of the magnetic rod sleeve 4. A second sealing block 8 is fixedly installed in the inner cavity of the connection sleeve 2. One side of the second sealing block 8 is in contact with the inner cavity of the magnetic rod sleeve 4. The bottom of the extraction tube body 1 is provided with two tangent arcs. The two tangent arcs can also be set as standard semi-circles or single arcs or cones.
[0037] During the implementation process, the design of this consumable adds an intersecting structure and performs the stirring and mixing action in a nearly sealed state, which can effectively prevent air exchange during the stirring and mixing process, keep the aerosol air containing nucleic acid molecules inside the tube, avoid cross-contamination. This consumable has a clamping and completely sealed design. After clamping, the magnetic rod sleeve 4 and the extraction tube body 1 are completely sealed and do not separate, which can effectively prevent the mixed liquid from volatilizing outside the tube, and at the same time avoid the exchange of aerosol air containing nucleic acid molecules inside the tube and the outside air. In the assembly line operation, when the stirring rod drives the magnetic rod sleeve 4 to complete the stirring work, the stirring rod separates from the magnetic rod sleeve 4, and the magnetic rod sleeve 4, the extraction tube body 1, and the mixed liquid remain at the lysis / binding position to continue lysis / binding. The stirring rod can move to a new magnetic rod sleeve 4, a new extraction tube body 1, and a new sample mixed liquid for stirring and mixing. In the same time, the mixing and stirring work of multiple samples can be carried out, improving the speed of nucleic acid extraction. This consumable can fasten the magnetic rod sleeve 4 and the extraction tube body 1, which is not only highly controllable, reducing instrument failures, but also the sealed state can prevent the accidental pouring out of the mixed liquid and prevent accidents from occurring.
[0038] Example 2
[0039] The extraction tube cap assembly 3 includes an extraction tube cap body 301. The extraction tube cap body 301 is arranged in the inner cavity of the connecting sleeve 2. A top cover 302 is fixedly installed at the top of the extraction tube cap body 301. A sealing sleeve 303 is fixedly installed at the bottom of the extraction tube cap body 301. A third sealing block 304 is fixedly installed on the surface of the sealing sleeve 303. A sealing ring 305 is arranged on the surface of the extraction tube cap body 301, and the size of the sealing ring 305 is adapted to the size of the sealing groove 6.
[0040] During the implementation process, when it is necessary to transfer and analyze the nucleic acid product in the extraction tube body 1, the extraction tube cap body 301 is fastened to the extraction tube body 1 and then transferred, which can effectively avoid the exchange of aerosol air containing nucleic acid molecules inside the extraction tube body 1 and the outside air, and also avoid cross-contamination.
[0041] During use, the design of this consumable adds an intersecting structure and performs the stirring and mixing action in a nearly sealed state, which can effectively prevent air exchange during the stirring and mixing process, keep the aerosol air containing nucleic acid molecules inside the tube, avoid cross-contamination. This consumable has a clamping and completely sealed design. After clamping, the magnetic rod sleeve 4 and the extraction tube body 1 are completely sealed and do not separate, which can effectively prevent the mixed liquid from volatilizing outside the tube and at the same time avoid the exchange of aerosol air containing nucleic acid molecules inside the tube and the air outside the tube. In the assembly line operation, when the stirring rod drives the magnetic rod sleeve 4 to complete the stirring work, the stirring rod separates from the magnetic rod sleeve 4, and the magnetic rod sleeve 4, the extraction tube body 1, and the mixed liquid remain at the lysis / binding position to continue lysis / binding. The stirring rod can move to a new magnetic rod sleeve 4, a new extraction tube body 1, and a new sample mixed liquid for stirring and mixing. Within the same time, the mixing and stirring work of multiple samples can be carried out, improving the speed of nucleic acid extraction. This consumable can fasten the magnetic rod sleeve 4 and the extraction tube body 1, which is not only highly controllable, reduces instrument failures, but also the sealed state can prevent the accidental pouring out of the mixed liquid and prevent accidents. When it is necessary to transfer and analyze the nucleic acid product in the extraction tube body 1, fasten the extraction tube cover body 301 to the extraction tube body 1 and then transfer it, which can effectively avoid the exchange of aerosol air containing nucleic acid molecules inside the extraction tube body 1 and the air outside the tube and also avoid cross-contamination.
[0042] The above embodiments are only used to illustrate the present invention and do not limit the technical solutions described in the present invention. Although this specification has described the present invention in detail with reference to the above respective embodiments, the present invention is not limited to the above specific embodiments. Therefore, any modification or equivalent replacement to the present invention; and all technical solutions and their improvements that do not deviate from the spirit and scope of the invention are covered by the scope of the claims of the present invention.
Claims
1. A cross-contamination-resistant nucleic acid extraction consumable, comprising an extraction tube body (1), characterized in that: A connecting sleeve (2) is fixedly mounted on the top of the extraction tube body (1); an extraction tube cover assembly (3) is arranged in the inner cavity of the connecting sleeve (2); a magnetic rod sleeve (4) is arranged in the inner cavity of the connecting sleeve (2); a magnetic rod body (5) is fixedly mounted on the bottom of the magnetic rod sleeve (4); and a sealing groove (6) is provided in the inner cavity of the connecting sleeve (2).
2. The cross-contamination-resistant nucleic acid extraction consumable according to claim 1, characterized in that: The extraction tube cover assembly (3) comprises an extraction tube cover body (301), wherein the extraction tube cover body (301) is arranged in the inner cavity of the connecting sleeve (2), a top cover (302) is fixedly installed on the top of the extraction tube cover body (301), a sealing sleeve (303) is fixedly installed on the bottom of the extraction tube cover body (301), and a third sealing block (304) is fixedly installed on the surface of the sealing sleeve (303).
3. A cross-contamination-resistant nucleic acid extraction consumable according to claim 2, characterized in that: A sealing ring (305) is provided on the surface of the extraction tube cover body (301), and the size of the sealing ring (305) is adapted to the size of the sealing groove (6).
4. The cross-contamination-resistant nucleic acid extraction consumable according to claim 1, characterized in that: A first sealing block (7) is fixedly mounted in the inner cavity of the connecting sleeve (2), and one side of the first sealing block (7) is in contact with the surface of the magnetic rod sleeve (4).
5. The cross-contamination-resistant nucleic acid extraction consumable according to claim 1, characterized in that: A second sealing block (8) is fixedly mounted in the inner cavity of the connecting sleeve (2), and one side of the second sealing block (8) is in contact with the inner cavity of the magnetic rod sleeve (4).
6. The cross-contamination-resistant nucleic acid extraction consumable according to claim 1, characterized in that: The bottom of the extraction pipe body (1) is arranged in two tangent arcs.