Low-temperature biological tissue dispersing and homogenizing device

Abstract

The utility model relates to the technical field of biopharmaceutical auxiliary instruments, in particular to a low-temperature biological tissue dispersing and homogenizing device which comprises a frame body and a homogenizer, the frame body comprises a base, a vertical pipe arranged on the base, a lower support used for fixing an EP pipe and an upper support arranged above the lower support and used for placing the homogenizer, and the vertical pipe is arranged on the base. The upper support and the lower support are both arranged on the vertical pipe; the EP pipe cooling device further comprises a cooling pipe assembly and a cooling liquid circulating pump, the cooling pipe assembly is arranged on the outer wall of the EP pipe in a sleeving mode, and the cooling liquid circulating pump is communicated with the cooling pipe assembly so as to circulate cooling liquid in the cooling pipe assembly. The problem that the low-temperature environment is affected by heat generated in the homogenizing process of biological tissue is solved, and the low-temperature grinding effect is improved.

Description

Technical Field

[0001] The present application relates to the technical field of biopharmaceutical auxiliary instruments, and in particular to a low-temperature biological tissue dispersion and homogenization device. Background Art

[0002] A low-temperature biological tissue dispersion and homogenization device is a device that can perform high-speed grinding and homogenization of biological tissues under low-temperature conditions. It mainly uses mechanical impact force and physical friction force under low-temperature conditions to achieve tissue fragmentation and homogenization. The low-temperature biological tissue dispersion and homogenization device is widely used in the processing of biological samples in the fields of biology, medicine, pharmacy, etc. Especially when extracting DNA, RNA, and proteins, low-temperature grinding can effectively inhibit nucleic acid degradation and retain protein activity, which is crucial for subsequent analysis and research.

[0003] In the related art, a manual grinder for small samples requires putting biological tissues into an EP tube and inserting a rod-shaped plastic homogenizer into the tube to manually grind the biological tissues.

[0004] In view of the above-mentioned related art, during the process of the homogenizer grinding biological tissues, to ensure sufficient grinding, the cutter head needs to collide fully with the biological tissues. However, the friction generated by the collision easily causes heat to be generated on the cutter head. At the same time, heat is also generated by the mutual friction between the biological tissue sample and the EP tube wall, resulting in the influence on the low-temperature grinding effect. Summary of the Utility Model

[0005] In order to improve the problem that the heat generated during the homogenization of biological tissues affects the low-temperature environment and improve the low-temperature grinding effect, the present application provides a low-temperature biological tissue dispersion and homogenization device.

[0006] A low-temperature biological tissue dispersion and homogenization device provided by the present application adopts the following technical solutions:

[0007] A low-temperature biological tissue dispersion and homogenization device includes a frame body and a homogenizer. The frame body includes a base, a vertical pipe provided on the base, a lower support for fixing an EP tube, and an upper support provided above the lower support for placing the homogenizer. Both the upper support and the lower support are provided on the vertical pipe;

[0008] It further includes a cooling pipe assembly and a coolant circulation pump. The cooling pipe assembly is sleeved on the outer wall of the EP tube, and the coolant circulation pump is connected to the cooling pipe assembly to circulate the coolant in the cooling pipe assembly.

[0009] By adopting the above technical solution, the cooling pipe assembly is sleeved on the outer wall of the EP tube, and in combination with the coolant circulation pump, the coolant in the cooling pipe assembly is continuously circulated, which can reduce the heat generated during the grinding process due to mechanical impact force and physical friction force, help maintain the low-temperature state of the grinding environment, thereby effectively inhibiting nucleic acid degradation and retaining protein activity, and improving the processing quality of biological samples. Grinding under low-temperature conditions can reduce the thermal denaturation of biological tissues, maintain the stability and activity of biological molecules, and the low-temperature environment also helps reduce the viscosity of biological tissues, making the grinding more uniform and sufficient.

[0010] Further, the cooling pipe assembly includes a plurality of cooling ring pipes arranged adjacent to each other along the length direction of the EP tube, a liquid inlet main pipe and a liquid discharge main pipe for connecting with the coolant circulation pump, and each of the cooling ring pipes is circumferentially wound around the outside of the EP tube;

[0011] Each of the cooling ring pipes has a liquid inlet end and a liquid discharge end, the coolant circulation pump is provided with an output end and a recovery end, one end of the liquid inlet main pipe is connected to the liquid inlet end of each of the cooling ring pipes, the end of the liquid inlet main pipe away from the cooling ring pipe is connected to the output end, one end of the liquid discharge main pipe is connected to the liquid discharge end of each of the cooling ring pipes, and the end of the liquid discharge main pipe away from the cooling ring pipe is connected to the recovery end.

[0012] By adopting the above technical solution, a plurality of cooling ring pipes are circumferentially wound around the outside of the EP tube, making the temperature distribution around the EP tube more uniform. The coolant circulation pump is connected to the liquid inlet ends and liquid discharge ends of each cooling ring pipe through the liquid inlet main pipe and the liquid discharge main pipe, forming an efficient coolant circulation system to ensure that the coolant can flow through each cooling ring pipe quickly and evenly during the circulation process, realizing rapid cooling and continuous cooling. Each cooling ring pipe independently cools a part of the EP tube, avoiding local overheating or insufficient cooling.

[0013] Further, a heat dissipation patch is provided between the outer wall of each cooling ring pipe and the EP tube, the heat dissipation patch is sleeved on the outer wall of the EP tube, and each cooling ring pipe is tightly abutted against the heat dissipation patch.

[0014] By adopting the above technical solution, the heat dissipation patch is sleeved on the outer wall of the EP tube and is in close contact with the cooling ring pipe. On the one hand, the heat dissipation patch can increase the contact area between the cooling ring pipe and the EP tube, and on the other hand, the heat dissipation patch has good heat conduction performance, which can quickly conduct the heat on the outer wall of the EP tube to the cooling ring pipe, helping to quickly reduce the temperature of the EP tube and the biological tissue inside it, and improving the cooling efficiency.

[0015] Further, the homogenizer includes a stirring rod and a driving head, the stirring rod is installed on the driving head and is driven by the driving head to rotate axially;

[0016] The upper support is provided with a placement groove for the driving head to be snapped into, and a through hole for the stirring rod to pass through is formed at the central position of the placement groove of the upper support.

[0017] By adopting the above technical solution, the stirring rod is axially rotated by the driving head, so as to grind and homogenize the biological tissue in the EP tube. The upper support is provided with a placement groove for the driving head to be snapped into, so that the homogenizer can be quickly and accurately positioned on the upper support, avoiding poor grinding effect caused by inaccurate position. A through hole for the stirring rod to pass through is formed at the central position of the placement groove, and the stirring rod can pass through the upper support during rotation and enter the EP tube to grind the biological tissue.

[0018] Furthermore, a magnetic block is arranged at the bottom of the placement groove, and a metal sheet for mutually adsorbing with the magnetic block is arranged on one side of the driving head close to the bottom of the placement groove.

[0019] By adopting the above technical solution, the magnetic block and the metal sheet mutually adsorb, ensuring the stable installation of the driving head in the placement groove, preventing the homogenizer from loosening due to vibration or external force during use, and improving the operation stability of the equipment.

[0020] Furthermore, a rubber ring for increasing friction is arranged on the inner side wall of the placement groove.

[0021] By adopting the above technical solution, the rubber ring increases the friction between the inner side wall of the placement groove and the driving head, making the driving head more stable in the placement groove, helping to reduce the relative movement between the driving head and the placement groove, ensuring that the homogenizer can also maintain an accurate position during high-speed rotation, and thus improving the grinding effect.

[0022] Furthermore, the lower support is provided with a placement hole for the EP tube to be snapped into, and the central axis of the placement hole is collinear with the central axis of the through hole.

[0023] By adopting the above technical solution, the placement hole on the lower support provides stable support for the EP tube, preventing the EP tube from shaking due to vibration during the homogenization process, helping to keep the position of the EP tube stable. The central axes of the placement hole and the through hole are collinear. When the EP tube is snapped into the placement hole, its axis will automatically align with the rotation axis of the stirring rod, ensuring that the stirring rod can accurately extend into the EP tube to uniformly and effectively grind the biological tissue.

[0024] Furthermore, a groove matching the bottom of the EP tube is formed on the base.

[0025] By adopting the above technical solution, the shape and size of the groove match the bottom of the EP tube, enabling the EP tube to be automatically aligned to the correct position when placed. The tight fit between the groove and the bottom of the EP tube makes the placement of the EP tube on the base more stable, reducing the wobbling caused by vibration or external forces.

[0026] Furthermore, the base is provided with rubber pads in the groove for increasing friction.

[0027] By adopting the above technical solution, the rubber pads can increase the friction between the bottom of the EP tube and the groove, preventing relative movement between the EP tube and the base, and making the fixation of the EP tube on the base more stable.

[0028] Furthermore, a plurality of adjustment holes for adjusting the positions of the upper support and the lower support are spaced along the length direction on the riser pipe, and connecting pieces for fixing the upper support and the lower support are inserted into the adjustment holes on the riser pipe.

[0029] By adopting the above technical solution, the arrangement of multiple adjustment holes on the riser pipe enables the operator to select appropriate adjustment holes for the upper support and the lower support respectively for fixation, flexibly adjust the relative position between the EP tube and the stirring rod according to experimental requirements, which helps to optimize the grinding effect and meet the processing requirements of different biological tissue samples. The connecting pieces firmly fix the upper support and the lower support on the riser pipe, maintaining the overall stability of the experimental system and improving the grinding efficiency.

[0030] In summary, the present application includes at least one of the following beneficial technical effects:

[0031] 1. The cooling tube assembly is sleeved on the outer wall of the EP tube, and the coolant circulation pump continuously circulates the coolant, effectively reducing the heat generated during the grinding process due to mechanical impact force and physical friction force, maintaining the low-temperature state of the grinding environment, which helps to inhibit nucleic acid degradation and retain protein activity, thereby improving the processing quality of biological samples. The low-temperature condition reduces the thermal denaturation of biological tissues, maintains the stability and activity of biological molecules, and at the same time reduces the viscosity of biological tissues, making the grinding process more uniform and sufficient;

[0032] 2. The upper support is provided with a placement groove for the driving head to be inserted. The mutual adsorption of the magnet and the metal sheet ensures the stable installation of the driving head in the placement groove. Combined with the rubber ring on the inner wall of the placement groove for increasing friction, it prevents the homogenizer from loosening during use due to vibration or external forces, improves the operation stability of the equipment, ensures that the homogenizer can also maintain an accurate position during high-speed rotation, and thus improves the grinding effect;

[0033] 3. Adjustment holes are spaced along the length of the riser pipe, which can flexibly adjust the positions of the upper support and the lower support on the riser pipe, thereby adjusting the relative position between the EP pipe and the stirring rod. The placement hole on the lower support provides stable support for the EP pipe, preventing the EP pipe from shaking during the homogenization process. A groove matching the bottom of the EP pipe is opened on the base, further enhancing the stability of the EP pipe on the base, reducing the shaking caused by vibration or external forces, and improving the overall stability of the experimental system. Description of the Drawings

[0034] Figure 1 is the overall structural schematic diagram of a low-temperature biological tissue dispersion and homogenization device according to an embodiment of the present application Figure 1 .

[0035] Figure 2 is the overall structural schematic diagram of a low-temperature biological tissue dispersion and homogenization device according to an embodiment of the present application Figure 2 .

[0036] Figure 3 is the exploded view of the structure of the upper support according to an embodiment of the present application.

[0037] Figure 4 is the overall structural schematic diagram of the homogenizer according to an embodiment of the present application.

[0038] Figure 5 is the exploded view of the structure of the bottom plate and the lower support according to an embodiment of the present application.

[0039] Figure 6 is the overall structural schematic diagram of the cooling pipe assembly and the coolant circulation pump according to an embodiment of the present application.

[0040] Figure 7 is the overall structural schematic diagram of the cooling ring pipe according to an embodiment of the present application.

[0041] Description of the reference numerals: 1, frame; 11, base; 111, groove; 112, rubber pad; 12, riser pipe; 121, adjustment hole; 122, connecting piece; 13, lower support; 131, placement hole; 14, upper support; 141, placement groove; 1411, magnet; 1412, rubber ring; 142, through hole; 2, homogenizer; 21, stirring rod; 22, drive head; 221, metal sheet; 3, cooling pipe assembly; 31, cooling ring pipe; 311, liquid inlet end; 312, liquid discharge end; 32, liquid inlet main pipe; 33, liquid discharge main pipe; 4, coolant circulation pump; 41, output end; 42, recovery end; 5, heat dissipation patch. Detailed Description of the Embodiment

[0042] In order to make the purpose, technical solutions and advantages of the present application clearer and more understandable, the following will further describe the present application in detail with reference to the Figures 1-6 drawings and embodiments.

[0043] The terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. in this embodiment indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. The disclosed embodiments of this application can be arranged in different directions. These terms indicating directions are only for illustration and not regarded as limitations. For example, "upper" and "lower" are not limited to the directions opposite to or consistent with the direction of gravity.

[0044] This embodiment of the present application discloses a low-temperature biological tissue dispersion and homogenization device. Refer to Figure 1 , the low-temperature biological tissue dispersion and homogenization device includes a frame body 1, a homogenizer 2, a cooling tube assembly 3 and a cooling circulation pump. The homogenizer 2 and the EP tube 0 are both arranged on the frame body 1. The cooling tube assembly 3 is wound around the outside of the EP tube 0 to cool the heat generated during the homogenization process. The cooling circulation pump is connected to the cooling tube assembly 3 to circulate the coolant in the cooling tube assembly 3.

[0045] Refer to Figure 1 and Figure 2 , the frame body 1 includes a base 11, a vertical pipe 12, a lower support 13 and an upper support 14. The base 11 is placed horizontally. The number of the vertical pipes 12 is two. Both vertical pipes 12 are vertically and fixedly connected to one side of the base 11. The upper support 14 and the lower support 13 are arranged at intervals up and down on the vertical pipe 12. Combining Figure 3 , both the upper support 14 and the lower support 13 are vertically penetrated by the vertical pipe 12. Connecting pieces 122 fixed by the user are penetrated through the sides of the upper support 14 and the lower support 13. In this embodiment, the number of the connecting pieces 122 on the upper support 14 and the lower support 13 is two. A plurality of adjusting holes 121 are arranged at intervals along the length direction on one side of the vertical pipe 12. When the upper support 14 / lower support 13 is adjusted to the required installation position, the connecting piece 122 passes through the side of the upper support 14 / lower support 13 and enters the corresponding adjusting hole 121 to fixedly connect the upper support 14 / lower support 13 to the vertical pipe 12.

[0046] Refer to Figure 2 and Figure 3 , the EP tube 0 is arranged on the base 11 and the lower support 13, and the homogenizer 2 is arranged on the upper support 14. The homogenizer 2 includes a stirring rod 21 and a driving head 22 for driving the axial rotation of the stirring rod 21. The stirring rod 21 is rotatably connected to the driving head 22. One end of the stirring rod 21 away from the driving head 22 extends into the EP tube 0 for homogenization operation.

[0047] Reference Figure 3 and Figure 4 , a placement groove 141 for fixing the driving head 22 is provided on the upper support 14, and a through hole 142 for the stirring rod 21 to pass through is provided at the central position of the placement groove 141. Four magnetic blocks 1411 are circumferentially arranged at intervals at the bottom of the placement groove 141. A metal sheet 221 is fixedly connected to one side of the driving head 22 close to the bottom of the placement groove 141. The metal sheet 221 in this embodiment is circular. When the driving head 22 is inserted into the placement groove 141, the metal sheet 221 adsorbs to each magnetic block 1411 to fix the driving head 22. To prevent the driving head 22 from rotating relative to the upper support 14 in the placement groove 141 during operation, a rubber ring 1412 is fixedly connected to the inner wall of the placement groove 141. The setting of the rubber ring 1412 can also buffer the vibration of the driving head 22 to improve the stability of the homogenization operation.

[0048] Reference Figure 2 and Figure 5 , a placement hole 131 matching the outer diameter of the EP tube 0 is provided on the lower support 13, and the central axis of the placement hole 131 is collinear with the central axis of the through hole 142, which is convenient for the automatic alignment of the stirring rod 21 and the EP tube 0. A groove 111 matching the bottom of the EP tube 0 is provided on the base 11, and a rubber pad 112 is provided in the groove 111. The rubber pad 112 is fixedly connected to the base 11. When the EP tube 0 is inserted into the placement hole 131, the edge of the EP tube 0 can abut against the upper surface of the lower support 13, the bottom of the EP tube 0 is inserted into the groove 111 and abuts against the rubber pad 112. The rubber pad 112 can increase the friction between the EP tube 0 and the base 11 on the one hand, and absorb the vibration generated during the homogenization process on the other hand, improving the stability of the homogenization operation.

[0049] Reference Figure 6 and Figure 7 , the cooling pipe assembly 3 includes a cooling ring pipe 31, a liquid inlet main pipe 32 and a liquid discharge main pipe 33. The number of cooling ring pipes 31 is several and they are arranged adjacent to each other along the length direction of the EP tube 0. Each cooling ring pipe 31 is sleeved outside the EP tube 0. The cooling ring pipe 31 has a liquid inlet end 311 and a liquid discharge end 312. The liquid inlet main pipe 32 is connected to the liquid inlet end 311 of each cooling ring pipe 31, and the liquid discharge main pipe 33 is connected to the liquid discharge end 312 of each cooling ring pipe 31.

[0050] The coolant circulation pump 4 has an output end 41 and a recovery end 42. One end of the liquid inlet main pipe 32 far from the cooling ring pipe 31 is connected to the output end 41 of the coolant circulation pump 4, and one end of the liquid discharge main pipe 33 far from the cooling ring pipe 31 is connected to the recovery end 42 of the coolant circulation pump 4. Combining Figure 5, a cylindrical heat dissipation patch 5 is sleeved and fixedly connected to the outer wall of the EP tube 0. In this embodiment, the heat dissipation patch 5 is preferably made of a metal material with good thermal conductivity, and each cooling loop tube 31 abuts against the outer wall of the heat dissipation patch 5.

[0051] The implementation principle of a low-temperature biological tissue dispersion and homogenization device according to an embodiment of the present application is as follows: The device mainly consists of a frame 1, a homogenizer 2, a cooling tube assembly 3 and a cooling circulation pump. The frame 1 includes a base 11, a vertical pipe 12, a lower support 13 and an upper support 14. The EP tube 0 is arranged on the base 11 and the lower support 13. The placement hole 131 on the lower support 13 matches the outer diameter of the EP tube 0 to provide stable support for the EP tube 0. The groove 111 on the base 11 fits tightly with the bottom of the EP tube 0, and the rubber pad 112 is used to increase friction and absorb vibration. The homogenizer 2 is installed on the upper support 14. The homogenizer 2 consists of a stirring rod 21 and a driving head 22. The driving head 22 is fixed on the upper support 14 through the placement groove 141 and is firmly connected through the adsorption of the magnetic block 1411 and the metal sheet 221. The stirring rod 21 rotates axially under the drive of the driving head 22, and its end extends into the EP tube 0 to grind and homogenize biological tissues.

[0052] The cooling tube assembly 3 includes a liquid inlet main pipe 32, a liquid discharge main pipe 33 and a plurality of cooling loop tubes 31. The cooling loop tubes 31 are arranged adjacent to each other along the length direction of the EP tube 0 and are sleeved on the heat dissipation patch 5 arranged outside the EP tube 0. The coolant enters each cooling loop tube 31 through the liquid inlet main pipe 32, absorbs heat and then flows back to the coolant circulation pump 4 through the liquid discharge main pipe 33, forming a closed-loop coolant circulation system. During the circulation process, the coolant continuously takes away the heat generated by the grinding of the EP tube 0 and the biological tissue inside it. Through the good thermal conductivity of the heat dissipation patch 5, the heat is quickly conducted to the cooling loop tubes 31 and taken away by the coolant. This enables the homogenization process to be carried out in a low-temperature environment, which helps to inhibit nucleic acid degradation, retain protein activity, and reduce the thermal denaturation of biological tissues.

[0053] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of the present application should be covered within the protection scope of the present application.

Claims

1. A low-temperature biological tissue dispersion and homogenization device, comprising a frame (1) and a homogenizer (2), characterized in that: The frame (1) comprises a base (11), a vertical pipe (12) arranged on the base (11), a lower support (13) for fixing the EP tube, and an upper support (14) arranged above the lower support (13) for placing the homogenizer (2), wherein the upper support (14) and the lower support (13) are both arranged on the vertical pipe (12); It also includes a cooling pipe assembly (3) and a coolant circulation pump (4), wherein the cooling pipe assembly (3) is sleeved on the outer wall of the EP pipe, and the coolant circulation pump (4) is connected to the cooling pipe assembly (3) to circulate the coolant in the cooling pipe assembly (3); The cooling pipe assembly (3) comprises a plurality of cooling ring pipes (31) arranged adjacent to each other along the length direction of the EP pipe, a liquid inlet main pipe (32) and a liquid discharge main pipe (33) for connecting to the cooling liquid circulation pump (4), and each of the cooling ring pipes (31) is circumferentially arranged outside the EP pipe; Each of the cooling loop pipes (31) has a liquid inlet end (311) and a liquid discharge end (312); the cooling liquid circulation pump (4) is provided with an output end (41) and a recovery end (42); one end of the liquid inlet main pipe (32) is connected to the liquid inlet end (311) of each of the cooling loop pipes (31); one end of the liquid inlet main pipe (32) away from the cooling loop pipe (31) is connected to the output end (41); one end of the liquid discharge main pipe (33) is connected to the liquid discharge end (312) of each of the cooling loop pipes (31); and one end of the liquid discharge main pipe (33) away from the cooling loop pipe (31) is connected to the recovery end (42).

2. A low-temperature biological tissue dispersion and homogenization device according to claim 1, characterized in that: A heat dissipation patch (5) is provided between each cooling ring tube (31) and the outer wall of the EP tube. The heat dissipation patch (5) is sleeved on the outer wall of the EP tube. Each cooling ring tube (31) and the heat dissipation patch (5) are tightly pressed against each other.

3. A low-temperature biological tissue dispersion and homogenization device according to claim 1, characterized in that: The homogenizer (2) comprises a stirring rod (21) and a driving head (22), wherein the stirring rod (21) is mounted on the driving head (22) and driven by the driving head (22) to perform axial rotation; The upper support (14) is provided with a placement groove (141) for the drive head (22) to be inserted into, and the upper support (14) is provided with a through hole (142) for the stirring rod (21) to pass through at the center position of the placement groove (141).

4. A low-temperature biological tissue dispersion and homogenization device according to claim 3, characterized in that: The placement groove (141) is provided with a magnetic block (1411) at the bottom of the groove, and the drive head (22) is provided with a metal sheet (221) for mutual adsorption with the magnetic block (1411) at one side close to the bottom of the placement groove (141).

5. A low-temperature biological tissue dispersion and homogenization device according to claim 4, characterized in that: A rubber ring (1412) for increasing friction is provided on the inner side wall of the placement groove (141).

6. A low-temperature biological tissue dispersion and homogenization device according to claim 4, characterized in that: The lower support (13) is provided with a placement hole (131) for the EP tube to be inserted into, and the central axis of the placement hole (131) is arranged colinearly with the central axis of the through hole (142).

7. The low-temperature biological tissue dispersion and homogenization device according to claim 1, characterized in that: The base (11) is provided with a groove (111) matching the bottom of the EP tube.

8. A low-temperature biological tissue dispersion and homogenization device according to claim 7, characterized in that: The base (11) is provided with a rubber pad (112) in the groove (111) for increasing friction.

9. A low-temperature biological tissue dispersion and homogenization device according to claim 1, characterized in that: The vertical pipe (12) is provided with a plurality of adjustment holes (121) spaced apart along the length direction for adjusting the positions of the upper support (14) and the lower support (13); and the vertical pipe (12) is provided with connecting pieces (122) passing through the adjustment holes (121) for fixing the upper support (14) and the lower support (13).

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