Counting plate for microorganisms and cells
By designing a counting plate with multiple counting pools, the problem of low cell counting efficiency and accuracy in traditional counting plates in biomedical research is solved, and the effect of improving cell counting efficiency and accuracy is achieved.
Patent Information
- Application Number
- CN202421853379.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Priority Date
- 2024-01-29
- Filing Date
- 2024-08-01
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-08-01
AI Technical Summary
In the prior art, there are limitations in the size and depth of the counting pool of traditional counting plates, resulting in low efficiency and accuracy of cell counting in biomedical research.
A counting plate suitable for microorganisms and cells is designed. The body of the counting plate includes a counting region extending in the same straight line direction and two non-counting regions. The counting region is provided with a plurality of counting cells, and each adjacent two counting cells is separated by a spacer.
By increasing the number of count pools and detection areas, the one-time loading and detection rate are improved, enhancing the efficiency and accuracy of cell counts while ensuring a clear boundary between each count pool.
Smart Images
Figure CN223047518U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of cell counting, and more particularly, to a counting plate suitable for microorganisms and cells. Background Art
[0002] In the related art, the counting pools of traditional counting plates usually adopt certain sizes and depths to ensure consistency and comparability during the counting process of microorganisms or cells. Although the specifications have been basically finalized and standardized, there are still some problems, such as a small sample addition volume at one time, which limits the efficiency and accuracy of cell counting in biomedical research. Summary of the Utility Model
[0003] The purpose of this application is to provide a counting plate suitable for microorganisms and cells to solve the technical problems in the related art.
[0004] This application provides a counting plate suitable for microorganisms and cells. The counting plate includes a counting plate body, and the counting plate body includes a counting area and two non-counting areas extending along the same straight line direction. The counting area is located between the two non-counting areas, and the counting area is provided with a plurality of counting pools, and each adjacent two of the counting pools are separated by a spacer.
[0005] Optionally, the counting plate suitable for microorganisms and cells further includes a cover plate, and the cover plate is used to cover the plurality of counting pools and at least part of the two non-counting areas, and a cavity is formed between the cover plate and the counting pools.
[0006] Optionally, the length range of the cavity is 19.5 - 20.5 mm, the width range is 4 - 6 mm, and the height range is 0.01 mm - 1 mm.
[0007] Optionally, the counting plate suitable for microorganisms and cells further includes a sample addition part, and the sample addition part is used to add a cell suspension into the cavity.
[0008] Optionally, the sample addition part is in a ramp shape, and in the direction from the edge of the counting plate body in the width direction to the center direction, the height of the sample addition part gradually increases.
[0009] Optionally, the maximum height of the sample addition part is less than or equal to the height of the counting pool.
[0010] Optionally, the number of the sample addition parts is two, and the counting pools are located between the two sample addition parts.
[0011] Optionally, the sample addition part is provided with an identification body.
[0012] Optionally, at least one of the non-counting areas is provided with a direction identification.
[0013] Optionally, the number of the counting pools is six.
[0014] By arranging a plurality of counting pools in the counting area, the amount of sample added at one time can be increased, the detection area and the detection rate can be increased, so that the efficiency and accuracy of cell counting in biomedical research can be improved. At the same time, since a spacer is provided between two adjacent counting pools, a clear boundary can be ensured between each counting pool.
[0015] Other features and advantages of the present application will be described in detail in the following specific implementation section. Description of the Drawings
[0016] The drawings are used to provide a further understanding of the present application, and constitute a part of the specification, and are used to explain the present application together with the following specific implementation manners, but do not constitute a limitation to the present application. In the drawings:
[0017] Figure 1 is a schematic structural diagram of a counting plate suitable for counting microorganisms and cells provided by an exemplary embodiment of the present application;
[0018] Figure 2 is Figure 1 a schematic structural diagram of another perspective of;
[0019] Figure 3 is Figure 2 an enlarged schematic diagram of part A in;
[0020] Figure 4 is a schematic structural diagram of a counting plate body provided by an exemplary embodiment of the present application.
[0021] Description of the Reference Numerals
[0022] 10 - counting plate body; 11 - counting area; 12 - non-counting area; 13 - counting pool; 14 - sample adding part; 15 - identification body; 16 - direction identification; 17 - spacer; 20 - cover plate; 21 - covering area; 22 - hand-held area; 30 - cavity. Specific Embodiments
[0023] The following will describe in detail the specific implementation manners of the present application with reference to the drawings. It should be understood that the specific implementation manners described herein are only used to illustrate and explain the present application, and are not used to limit the present application.
[0024] In the present application, unless otherwise stated, the terms "upper" and "lower" refer to the orientation or positional relationship in which the product is normally placed during use, and can be understood as up and down along the direction of gravity, and also correspond to "upper" and "lower" in the drawings. In addition, it should be noted that the terms such as "first" and "second" are used to distinguish one element from another, and do not have an order or importance.
[0025] Figure 1 This is a schematic structural diagram of a counting plate suitable for microorganisms and cells shown in an exemplary embodiment of the present application. Figure 2 This is a schematic structural diagram of another perspective of a counting plate suitable for microorganisms and cells shown in an exemplary embodiment of the present application. Figure 3 For Figure 2 The enlarged schematic diagram of part A in Figure 4 This is a schematic structural diagram of a counting plate main body shown in an exemplary embodiment of the present application.
[0026] As Figure 1 and Figure 2 shown, the present application provides a counting plate suitable for microorganisms and cells. The counting plate includes a counting plate main body 10. The counting plate main body 10 includes a counting area 11 and two non-counting areas 1211 extending along the same straight line direction. The counting area 11 is located between the two non-counting areas 1211. The counting area 11 is provided with a plurality of counting pools 13. Each adjacent two counting pools 13 are separated by a spacer 17.
[0027] The shape of the counting plate main body 10 is not fixed and can be different shapes such as square, rectangular, etc., which are not specifically limited in the present application.
[0028] In the present application, by providing a plurality of counting pools 13 in the counting area 11, the amount of sample added at one time can be increased, the detection area and the detection rate can be increased, thereby improving the efficiency and accuracy of cell counting in biomedical research. At the same time, since there is a spacer 17 between each adjacent two counting pools 13, a clear boundary between each counting pool 13 can be ensured.
[0029] In the present application, the counting plate main body 10 can be made of glass. The edge of the glass can be polished by a process of straight-edge chamfering, making it safer and not easily scratching the user. The following embodiments are described by taking the counting plate main body 10 made of glass as an example.
[0030] Among them, the spacer 17 can be set to any suitable structure.
[0031] As Figure 2 shown, in one example, the spacer 17 can be a groove. By etching on the surface of the glass, a groove extending along the width direction of the counting plate main body 17 is formed, so that two adjacent counting pools 13 can be separated.
[0032] In one example, the spacer 17 can also be a strip (not shown in the figure). For example, tape, paper strip, etc. can be used, and the color of the strip is different from the color of the counting plate main body 10, so that the boundary between the spacer 17 and the counting pool 13 is clearly distinguishable.
[0033] In one example, the spacer 17 can also be a coating (not shown in the figure). For example, a layer of paint that can be different in color from the glass itself can be coated on the glass, and two adjacent counting cells 13 are separated by this coating. Due to the different extensions of the coating and the glass, the edge of the coating can also form a distinct boundary with the edge of the counting cell 13.
[0034] In one example, the spacer 17 can also be a pattern layer (not shown in the figure), that is, by forming a pattern on the surface of the glass, a distinct boundary can also be formed between the spacer 17 and the counting cell 13.
[0035] As Figure 1 shown, in the present application, the counting plate further includes a cover plate 20, and the cover plate 20 is used to cover a plurality of counting cells 13 and at least part of the two non-counting areas 1211, that is, the cover plate 20 will cover all the counting cells 13 and a part of the non-counting area 1211 at the same time. A cavity 30 is formed between the cover plate 20 and the counting cell 13. By forming the cavity 30 between the cover plate 20 and the counting cell 13, the cavity 30 can accommodate the cell suspension.
[0036] As Figure 1 、 Figure 2 、 Figure 3 shown, the counting area 11 and the non-counting area 1211 of the counting plate body 10 have different thicknesses, and the thickness of the counting area 11 is less than the thickness of the non-counting area 1211. Thus, when the cover plate 20 covers the two non-counting areas 1211, due to the different thicknesses of the counting area 11 and the non-counting area 1211, they will not fit together on the side of the cover plate 20 facing the counting cell 13, but are spaced apart by a certain distance, thereby forming the cavity 30.
[0037] It should be understood that the width of the cover plate 20 is greater than the length of the counting cell 13.
[0038] In this application, the length range of the cavity 30 is 19.5 - 20.5 mm, the width range is 4 - 6 mm, and the height range is between 0.01 mm - 1 mm. Specifically, the length of the cavity 30 can be 19.5, 19.6, 19.7, 19.8, 19.9, 20, 20.1, 20.2, 20.3, 20.4 or 20.5 mm, the width can be 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9 or 6.0 mm, and the height range is 0.01, 0.02, 0.03, 0.14, 0.1 mm or 1 mm. Specific values are not exhausted here. Among them, when the height range is about 0.1 mm, the counting plate is suitable for observing and counting cells, and when the height range is about 0.02 mm, the counting plate is suitable for observing and counting microorganisms.
[0039] The cavity 30 formed by the above dimensions can ensure that the cell suspension is evenly distributed in the counting pool 13 by siphon action, and each cavity 30 can accommodate 10 μL of cell suspension.
[0040] Among them, the cover plate 20 can be made of glass. The cover plate 20 is treated with safety corners, that is, the corners of the cover plate 20 are cut off so that its top corners form a rounded structure, thus avoiding hurting the user's palm.
[0041] Among them, the cover plate 20 is made of colorless and transparent glass for the user to observe the cell suspension.
[0042] As Figure 1 shown, in this application, the area of the cover plate 20 is smaller than the area of the counting plate body 10. The cover plate 20 includes a covering area 21 corresponding to the counting pool 13 and a holding area 22. Among them, the number of the holding areas 22 is two, and the covering area 21 is located between the two holding areas 22. The holding areas 22 are mainly used for the user to hold the two holding areas 22 with both hands respectively, so as to facilitate taking the cover plate 20 and improve the controllability and stability.
[0043] As Figure 1 shown, in this application, marking lines can also be set at both ends of the cover plate 20 to distinguish the holding area 22 and the covering area 21. The marking lines can be formed by etching, or other objects with the boundary of the holding area 22 and the covering area 21 can be adhered to the cover plate 20, which is convenient for the user to place and control correctly during use.
[0044] Since the cover plate 20 and the counting plate body 10 are designed separately, it is also convenient to clean the cover plate 20 and the counting plate body 10 separately, making the cleaning more thorough and clean.
[0045] AsFigure 2 and Figure 3 As shown in Figure 3 , in the embodiment of the present application, taking cell counting as an example, the counting plate further includes a sample loading part 14, and the sample loading part 14 is used to add the cell suspension into the cavity 30. When in use, a pipette can be used to aspirate the cell suspension, inject the cell suspension at the sample loading part 14, then cover the cover plate 20, and place the counting plate suitable for the counting of microorganisms and cells on the sample stage of the instrument for observation and automatic counting.
[0046] In the present application, the sample loading part 14 is in a slope shape, and in the direction from the edge of the counting plate main body 10 in the width direction to the center direction, the height of the sample loading part 14 gradually increases.
[0047] Since the height of the cavity 30 between the cover plate 20 and the counting pool 13 is small, between 0.01 mm and 1 mm, when the cell suspension is injected at the sample loading part 14 and the cover plate 20 is covered, the capillary force generated by the cavity 30 can suck the cell suspension at the sample loading part 14 into the cavity 30, thus playing the role of facilitating sample loading.
[0048] In the present application, in order to enable the cell suspension to enter the cavity 30 more smoothly, the inclination angle of the sample loading part 14 should not be too large. For example, the angle of the sample loading part 14 can be 20 - 40 degrees.
[0049] In the present application, the maximum height of the sample loading part 14 is less than or equal to the height of the counting pool 13, so that the cell suspension can smoothly enter the cavity 30.
[0050] In the present application, the number of the sample loading parts 14 is two, and the counting pool 13 is located between the two sample loading parts 14. In other words, each counting pool 13 and its corresponding two sample loading parts 14 extend along the width direction of the counting plate main body 10, so that the counting pool 13 is between the two sample loading parts 14. In this case, no matter from the left or the right of the counting pool 13, the cell suspension can be added into the counting pool 13. At the same time, when adding the cell suspension from the left and the right of the counting pool 13 simultaneously, the cell suspension can also be more evenly distributed in the cavity 30.
[0051] such as Figure 4 As shown in Figure 4 , in the present application, the sample loading part 14 is provided with an identification body 15. Since each sample loading part 14 has its corresponding counting pool 13, by setting the identification body 15 on the sample loading part 14, the counting pools 13 can be distinguished, which is convenient for users to identify and record the cells in each counting pool 13.
[0052] Among them, the identification body 15 can be set to any suitable structure.
[0053] In one example, the identification body 15 can be a number. For example, the identification body 15 can be 1, 2, 3, 4, 5, 6, etc.
[0054] In one example, the identification body 15 can be a letter. For example, the identification body 15 can be A, B, C, D, E, F, etc.
[0055] The identification body 15 can be set only on one sample adding part 14 corresponding to each counting cell 13, or can be set on two sample adding parts 14 corresponding to each counting cell 13 at the same time. The identification body 15 can be obtained by etching on the sample adding part 14, or can be formed by bonding the made numbers or letters on the sample adding part 14.
[0056] Such as Figure 4 As shown, in this application, at least one non-counting area 1211 is provided with a direction identifier 16, and this direction identifier 16 can provide direction guidance for placing a counting plate suitable for microorganisms and cells on the instrument sample stage. Among them, the direction identifier 16 can adopt an arrow. Similarly, since the counting plate body 10 can adopt glass, the direction identifier 16 can also be formed on the counting plate body 10 by etching, or can be formed by bonding the made direction identifier 16 on the counting plate body 10.
[0057] The number of the direction identifiers 16 can be one. When it is one, the direction identifier 16 is set in any one non-counting area 1211.
[0058] The number of the direction identifiers 16 can be two. When it is two, the two direction identifiers 16 are respectively set in one non-counting area 1211.
[0059] Such as Figure 4 As shown, in this application, the number of the counting cells 13 can be four, five, six or seven. By adopting multiple counting cells 13, the corresponding area is increased compared with the ordinary design. At the same time, it can also ensure that the field of view is clearer during counting, reduce external interference, and control the counting volume.
[0060] In one example, in order to prevent the cover plate 20 from moving relative to the counting plate body 10, a positioning groove (not shown in the figure) can also be opened on the side of the non-counting area 12 close to the counting area 11. The area of this positioning groove is equivalent to the area of the hand-held area 22 of the cover plate 20 and is slightly larger than the area of the hand-held area 22. Thus, the two hand-held areas 22 of the cover plate 20 can be respectively placed in the two positioning grooves, so as to limit the movement of the cover plate 20 on the horizontal plane of the counting plate body 10 through the positioning grooves.
[0061] The preferred embodiments of the present application have been described in detail above in conjunction with the accompanying drawings. However, the present application is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present application, various simple modifications can be made to the technical solutions of the present application, and these simple modifications all fall within the protection scope of the present application. In addition, it should be noted that, in the various specific technical features described in the above specific embodiments, they can be combined in any appropriate manner without conflict. To avoid unnecessary repetition, the present application will not separately describe various possible combination manners.
[0062] In addition, any combination can be made between various different embodiments of the present application, as long as it does not violate the idea of the present application, it should equally be regarded as the content claimed in the present application.
Claims
1. A counting plate suitable for microorganisms and cells, characterized in that: The counting board body (10) comprises a counting area (11) and two non-counting areas (12) extending in the same straight line direction, the counting area (11) is located between the two non-counting areas (12), the counting area (11) is provided with a plurality of counting pools (13), and each adjacent two counting pools (13) are separated by a spacer (17).
2. The counting plate suitable for microorganisms and cells according to claim 1, characterized in that: The counting plate suitable for microorganisms and cells further comprises a cover plate (20), wherein the cover plate (20) is used to cover the plurality of counting pools (13) and at least part of the two non-counting areas (12), and a cavity (30) is formed between the cover plate (20) and the counting pools (13).
3. The counting plate suitable for microorganisms and cells according to claim 2, characterized in that: The cavity (30) has a length ranging from 19.5 to 20.5 mm, a width ranging from 4 to 6 mm, and a height ranging from 0.01 mm to 1 mm.
4. The counting plate suitable for microorganisms and cells according to claim 2, characterized in that: The counting plate suitable for microorganisms and cells further comprises a sample adding portion (14), wherein the sample adding portion (14) is used to add a cell suspension into the cavity (30).
5. The counting plate suitable for microorganisms and cells according to claim 4, characterized in that: The sample adding portion (14) is in a slope shape and points from the edge in the width direction of the counting plate body (10) to the center direction, and the height of the sample adding portion (14) gradually increases.
6. The counting plate suitable for microorganisms and cells according to claim 5, characterized in that: The maximum height of the sample adding portion (14) is less than or equal to the height of the counting pool (13).
7. The counting plate suitable for microorganisms and cells according to claim 4, characterized in that: The number of the sample adding parts (14) is two, and the counting pool (13) is located between the two sample adding parts (14).
8. The counting plate suitable for microorganisms and cells according to claim 4, characterized in that: The sample adding portion (14) is provided with a marking body (15).
9. The counting chamber for microorganisms and cells according to claim 1, characterized in that: At least one of the non-counting areas (12) is provided with a direction mark (16).
10. The counting chamber for microorganisms and cells according to claim 1, characterized in that: The number of the counting pools (13) is six.