Detection device

Abstract

The embodiment of the utility model relates to a detection device, which comprises an amplification reaction unit at least comprising a sample inlet, a reaction chamber and a sealing cover, the sample inlet is configured to receive a sample provided for the amplification reaction unit, the reaction chamber is configured to provide a space for amplification reaction, and the sealing cover is configured to seal the sample inlet; the test paper reaction unit at least comprises a test strip for detecting an amplification product and a shell for accommodating the test strip, the test strip is used for detecting the amplification product which comes from the reaction chamber and is obtained through an amplification reaction, and the amplification reaction unit and the test strip reaction unit are arranged at a preset angle and are communicated through a first interface of the amplification reaction unit. According to the detection device provided by the utility model, amplification products generated in the amplification reaction unit can directly flow into the test paper reaction unit for detection by rotating for a preset angle, such as knocking for preset times, so that the pollution risk is remarkably reduced, and meanwhile, the structure is simple, the operation is simple, and the detection efficiency is high.

Description

Technical Field

[0001] Embodiments of the present utility model generally relate to the field of biological detection, and more particularly to a detection device. Background Art

[0002] Due to characteristics such as sensitivity, rapidity, and simple operation, nucleic acid amplification detection technology is widely used in aspects such as gene detection, auxiliary diagnosis of genetic diseases, and detection of infectious diseases to detect chemical components, virus species, etc. in samples. The process of nucleic acid amplification detection generally includes: amplifying the extracted nucleic acid and detecting the nucleic acid amplification product.

[0003] The disadvantage of traditional detection devices for detecting nucleic acid amplification products based on test strips is that it is necessary to transfer the amplification product to the test strip, which generates additional operation steps. At the same time, the transfer process is prone to aerosol contamination, resulting in the problem of environmental positivity. Summary of the Utility Model

[0004] The present utility model provides a detection device that can significantly reduce the risk of contamination, and at the same time has a simple structure, simple operation, and high detection efficiency.

[0005] According to a first aspect of the present utility model, there is provided a detection device, including: an amplification reaction unit, at least including a sample inlet, a reaction chamber, and a sealing cover, the sample inlet being configured to receive a sample provided to the amplification reaction unit, the reaction chamber being configured to provide a space for an amplification reaction, and the sealing cover being configured to seal the sample inlet; and a test strip reaction unit, at least including a test strip for detecting an amplification product and a housing for accommodating the test strip, the test strip being used to detect the amplification product obtained from the reaction chamber through an amplification reaction, wherein the amplification reaction unit and the test strip reaction unit are arranged at a preset angle and communicate through a first interface of the amplification reaction unit.

[0006] In some embodiments, the first interface is configured such that when the detection device is rotated by a predetermined angle and tapped a predetermined number of times, the amplification product obtained in the reaction chamber flows through the first interface to contact the test strip.

[0007] In some embodiments, the reaction chamber is provided with a first coupling portion for coupling with a second coupling portion provided on the sealing cover to fix the sealing cover.

[0008] In some embodiments, the amplification reaction unit further includes: an anti-pollution member configured at the sample inlet to prevent the reagents and / or samples in the amplification reaction unit from being contaminated.

[0009] In some embodiments, the amplification reaction unit further includes: a filtering unit, wherein a filter sheet is configured in the filtering unit to prevent the aerosol generated by the sample in the reaction chamber from escaping.

[0010] In some embodiments, the amplification reaction unit is detachably coupled to the test strip reaction unit.

[0011] In some embodiments, a first plug and a second plug are provided on a first side of the amplification reaction unit, and a first jack corresponding to the first plug and a second jack corresponding to the second plug are provided on a first side of the housing of the test strip reaction unit, and the amplification reaction unit is detachably coupled to the test strip reaction unit via the first plug and the second plug.

[0012] In some embodiments, a visual window is provided on a first side of the housing of the test strip reaction unit, and the visual window is configured to display the detection result status of the test strip.

[0013] In some embodiments, the housing of the test strip reaction unit includes an upper housing and a lower housing that are snap-fitted together, and a sealing strip is provided at the inner edge of the lower housing to prevent the amplified products in the test strip reaction unit from leaking out.

[0014] In some embodiments, the amplification reaction unit includes a plurality of reaction chambers.

[0015] In some embodiments, the amplification reaction unit including a plurality of reaction chambers is discrete or integrally formed.

[0016] In some embodiments, the test strip reaction unit includes a plurality of test strips.

[0017] In some embodiments, the number of the plurality of reaction chambers is the same as the number of the plurality of test strips, and each reaction chamber corresponds to one of the plurality of test strips, so that each test strip can detect the amplified products obtained in the corresponding reaction chamber.

[0018] In some embodiments, the housing of the test strip reaction unit is provided with a plurality of visual windows, and each visual window corresponds to one of the plurality of test strips for displaying the detection result status of the test strip.

[0019] In some embodiments, a microchannel is provided inside the housing of the test strip reaction unit. The liquid inlet end of the microchannel is connected to a first interface, and the liquid outlet end of the microchannel is connected to the first end of the test strip.

[0020] In some embodiments, a first port of the first interface is in communication with the reaction chamber in the amplification reaction unit, and a second port of the first interface is in communication with the liquid inlet end of the microchannel in the test strip reaction unit, so that the amplified products obtained in the reaction chamber flow through the first interface and into the microchannel, thereby coming into contact with the test strip.

[0021] In some embodiments, the test strip reaction unit includes a plurality of test strips, and the microchannel includes a plurality of liquid outlet ends, wherein each liquid outlet end is connected to the first end of one of the plurality of test strips.

[0022] In some embodiments, the amplification reaction unit includes a plurality of reaction chambers. Among them, the amplification products in the plurality of reaction chambers are mixed and then flow into the first interface and then into the microchannel to contact each of the plurality of test strips.

[0023] In some embodiments, the preset angle is any angle between 70° and 110°.

[0024] It should be understood that the content described in this part is not intended to identify the key or important features of the embodiments of the present invention, nor is it used to limit the scope of the present invention. Other features of the present invention will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In combination with the accompanying drawings and with reference to the following detailed description, the above and other features, advantages and aspects of the embodiments of the present invention will become more obvious. In the drawings, the same or similar reference numerals denote the same or similar elements.

[0026] Figure 1 A schematic diagram of a detection device according to a first embodiment of the present invention is shown.

[0027] Figure 2 An exploded view of a detection device according to a first embodiment of the present invention is shown.

[0028] Figure 3 A schematic structural diagram of an amplification reaction unit of a detection device according to an embodiment of the present invention is shown.

[0029] Figure 4 A schematic structural diagram of a test strip reaction unit of a detection device according to an embodiment of the present invention is shown.

[0030] Figure 5A A cross-sectional view of an amplification reaction unit of a detection device according to an embodiment of the present invention is shown.

[0031] Figure 5B A top view of an amplification reaction unit of a detection device according to an embodiment of the present invention is shown.

[0032] Figure 6A A cross-sectional view of a detection device placed in a first posture according to an embodiment of the present invention is shown.

[0033] Figure 6B A cross-sectional view of a detection device placed in a second posture according to an embodiment of the present invention is shown.

[0034] Figure 7 A schematic diagram of a detection device according to a second embodiment of the present invention is shown.

[0035] Figure 8 Shows a schematic diagram of a detection device according to a third embodiment of the present utility model.

[0036] Figure 9 Shows a schematic diagram of a detection device according to a fourth embodiment of the present utility model.

[0037] Figure 10 Shows a schematic diagram of a detection device according to a fifth embodiment of the present utility model. Detailed implementation manners

[0038] The following describes exemplary embodiments of the present utility model in conjunction with the accompanying drawings. Various details of the embodiments of the present utility model are included to facilitate understanding, and they should be considered merely exemplary. Therefore, those of ordinary skill in the art should recognize that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of the present utility model. Similarly, for the sake of clarity and conciseness, descriptions of well-known functions and structures are omitted below.

[0039] The term "including" and its variations used herein mean open inclusion, i.e., "including but not limited to". The term "based on" means "at least partially based on". The term "an exemplary embodiment" and "an embodiment" mean "at least one exemplary embodiment". The term "another embodiment" means "at least one additional embodiment". The terms "first", "second", etc. may refer to different or the same objects. There may be other explicit and implicit definitions below.

[0040] Due to characteristics such as sensitivity, rapidity, and simple operation, nucleic acid amplification detection technology is widely used in aspects such as gene detection, auxiliary diagnosis of genetic diseases, and detection of infectious diseases to detect chemical components, virus species, etc. in samples. The process of nucleic acid amplification detection generally includes: amplifying the extracted nucleic acid and detecting the nucleic acid amplification product.

[0041] Currently, among various detection devices for nucleic acid amplification detection, a common method includes using a test strip to detect the amplification product.

[0042] In traditional detection devices for detecting nucleic acid amplification products based on test strips, in order to avoid the reaction solution contacting the test strip when the amplification reaction is incomplete, the reaction unit for nucleic acid amplification and the detection unit for detecting the amplification product are usually separately arranged. After the amplification reaction is complete, the amplification product is then transferred from the reaction unit to the detection unit. Specifically, the extracted nucleic acid can be amplified in a separate container (such as a test tube) first, and then the obtained amplification product is dropped at a predetermined position on the test strip to determine the detection result according to the detection result state on the test strip.

[0043] However, in the above - mentioned solution, during the process of transferring the amplification product to the detection unit, it is easy to cause contamination of the amplification product, thus affecting the accuracy of the detection result. At the same time, the aerosol in the nucleic acid amplification product is also easy to diffuse into the surrounding air, causing environmental pollution.

[0044] In some other solutions related to the existing detection devices, the reaction unit and the detection unit are integrated in one device, and the control of the transfer of the amplification product from the reaction unit to the detection unit is achieved through structural design. However, in these solutions, the detection unit needs to be set so that the test strip therein is always in a flat state, so that the amplification product can drip onto the test strip based on gravity for detection. As a result, the structure of the detection device is complex and the detection efficiency is low.

[0045] In order to at least partially solve one or more of the above - mentioned problems and other potential problems, an exemplary embodiment of the present utility model provides a detection device, which includes an amplification reaction unit and a test strip reaction unit. The amplification reaction unit at least includes: a sample inlet configured to receive a sample provided to the amplification reaction unit, a reaction chamber configured to provide a space for the amplification reaction, and a sealing cover configured to seal the sample inlet. The test strip reaction unit at least includes: a test strip for detecting the amplification product, and a housing for accommodating the test strip, wherein the test strip is used to detect the amplification product obtained from the reaction chamber through the amplification reaction. In this detection device, by setting the amplification reaction unit and the test strip reaction unit at a preset angle and connecting them through a first interface, when the detection device is rotated by a predetermined angle and tapped a predetermined number of times, the amplification product generated in the amplification reaction unit can directly flow into the test strip reaction unit through the first interface for detection, significantly reducing the risk of contamination. At the same time, the structure is simple, the operation is easy, and the detection efficiency is high.

[0046] The following will be combined with Figures 1 to 10 Describe in detail the solution of the detection device according to the embodiment of the present utility model.

[0047] Figure 1 Fig. shows a schematic diagram of a detection device 100 according to a first embodiment of the present utility model.

[0048] As Figure 1 shown, the detection device 100 includes an amplification reaction unit 110 and a test strip reaction unit 120. The amplification reaction unit 110 and the test strip reaction unit 120 are set at a preset angle and connected through a first interface (not shown).

[0049] Regarding the preset angle, it can be any angle between 60° and 120°, and optionally, it can be any angle between 70° and 110°. In some embodiments, the preset angle can preferably be 90°.

[0050] According to the present utility model, during use, the detection device 100 can be placed in a first posture. In this first posture, the test strip (not shown) in the test strip reaction unit 120 is in a vertical state. After the amplification reaction is completed, the detection device 100 can be placed in a second posture by rotating a predetermined angle (such as 90°). In this second posture, the test strip (not shown) in the test strip reaction unit 120 is in a flat state, and the first interface is in a vertical state, so that the amplification product in the amplification reaction unit 110 can enter the test strip reaction unit 120 via the first interface (not shown) due to gravity, so as to be in contact with the test strip in the test strip reaction unit 120, thereby realizing the detection of the amplification product.

[0051] In summary, the detection device 100 provided by the present utility model can control the flow of liquid in the detection device by changing the posture of the detection device 100. Specifically, during the amplification process, the sample undergoes an amplification reaction in the amplification reaction unit 110 without flowing out and entering the test strip reaction unit 120; after the amplification reaction is completed, the detection device 100 can then be rotated by a predetermined angle and tapped a predetermined number of times, so that during the detection process, the amplification product obtained in the amplification reaction unit 110 can flow into the test strip reaction unit 120 due to gravity, thereby realizing the amplification detection of the sample. For example, in some embodiments, in response to the completion of the amplification reaction, the detection device 100 is rotated 90° and tapped, such as 3 times, so that the amplification product in the amplification reaction unit 110 can smoothly flow into the test strip reaction unit 120 via the first interface.

[0052] In still other embodiments, alternatively, in response to the detection device 100 being rotated by a predetermined angle, the amplification product obtained in the amplification reaction unit 110 can also flow into the test strip reaction unit 120 by other means such as stirring with a spindle-shaped stirrer, so as to realize the amplification detection of the sample.

[0053] The present utility model does not limit this.

[0054] Figure 2 Figures 4 to 5 show Figure 1 the specific structure of the detection device 100.

[0055] Figure 2 shows Figure 1 the exploded view of the detection device 100. It should be understood that the detection device 100 may further include additional units not shown and / or units shown may be omitted, and the scope of the present utility model is not limited in this regard.

[0056] Such as Figure 2As shown, the detection device 100 includes: an amplification reaction unit 110 and a test strip reaction unit 120. Among them, an amplification reaction is performed on the sample added to the amplification reaction unit 110, and the amplification product obtained in the amplification reaction unit 110 is detected in the test strip reaction unit 120.

[0057] Specifically, as Figure 2 shown, the amplification reaction unit 110 may include: a sample inlet 112, a reaction chamber 114, and a sealing cover 118. Regarding the sample inlet 112, it may be configured to receive the sample provided to the amplification reaction unit 110. Regarding the reaction chamber 114, it may be configured to provide a space for the amplification reaction. Regarding the sealing cover 118, it may be configured to seal the sample inlet, which will be described in detail below. According to an embodiment of the present invention, a sample (for example, extracted nucleic acid) may be added to the reaction chamber 114 via the sample inlet 112, and the reaction chamber 114 may be sealed with the sealing cover 118, so as to facilitate the amplification reaction of the sample with the reagents in the reaction chamber 114 and obtain an amplification product.

[0058] As Figure 2 shown, the amplification reaction unit 110 may further include an anti-pollution member 116 disposed at the sample inlet 112 for preventing the reagents and / or samples in the amplification reaction unit 110 from being contaminated. For example, in some embodiments, the anti-pollution member 115 may include a small hole, the diameter of which is smaller than the diameter of the pipette tip for sample addition, and the side wall around the small hole is convergent, such as a funnel shape. Thus, during the sample addition process, the pipette tip for sample addition can be limited by the small hole and its convergent side wall around it, avoiding contact between the pipette tip and the reagents in the reaction chamber 114 during sample addition, thereby causing reagent contamination. At the same time, the convergent side wall also helps the sample to better flow into the reaction chamber 114 for amplification reaction.

[0059] As Figure 2 shown, the amplification reaction unit 110 may further include a sealing cover 118 coupled to the reaction chamber 114 for sealing the sample inlet 112 after sample addition, thereby effectively preventing pollutants in the surrounding environment from entering the reaction chamber 114, and at the same time preventing the aerosol generated by the sample in the reaction chamber during the amplification reaction from diffusing into the surrounding air, causing environmental pollution. And, since the sealing cover 118 seals the aerosol escaping from the reaction chamber 114 at the sample inlet 112, causing the aerosol to accumulate and generate air pressure, it helps the sample at the sample inlet 112 to better flow into the following reaction chamber 114.

[0060] According to an embodiment of the present invention, in order to better fix the sealing cover 118 on the reaction chamber 114, components for coupling may be respectively provided on the sealing cover 118 and the reaction chamber 114, which will be described in detail below in combination with Figure 3 in detail.

[0061] As Figure 3 shown, the reaction chamber 114 is provided with a first coupling portion 212, and the sealing cover 118 is provided with a second coupling portion 214, wherein the second coupling portion 214 is correspondingly arranged with the first coupling portion 212. In some embodiments, the first coupling portion 212 may be in the form of a clamping groove, and the second coupling portion 214 may correspondingly be in the form of a snap, so that the first coupling portion 212 and the second coupling portion 214 are coupled by a snap connection manner, thereby fixing the sealing cover 118 on the reaction chamber 114 and sealing the sample inlet 112. It should be understood that the reaction chamber 114 and the sealing cover 118 may also be coupled by other appropriate means to achieve the effect of fixing the sealing cover 118 on the reaction chamber 114 and sealing the sample inlet 112, and the present utility model does not limit this.

[0062] In addition, in some embodiments, the coupling between the sealing cover 118 and the reaction chamber 114 is one-time and irreversible. That is to say, once the sealing cover 118 and the reaction chamber 114 are coupled through the second coupling portion 214 of the sealing cover 118 and the first coupling portion 212 of the reaction chamber 114, even after the amplification reaction is completed, it is impossible to open the sealing cover 118 by decoupling the first coupling portion 212 and the second coupling portion 214, thereby avoiding the contamination of the amplification product and environmental pollution caused by opening and closing the sealing cover 118.

[0063] According to an embodiment of the present utility model, the amplification reaction unit 110 may further include a filtering unit to prevent the aerosol generated by the sample in the reaction chamber from escaping. Referring to Figure 3 , the amplification reaction unit 110 may include a filtering unit 216, which is configured to communicate with the reaction chamber 114. According to an embodiment of the present utility model, a filter sheet may be disposed in the filtering unit 216 to prevent the aerosol entering the filtering unit 216 from the reaction chamber 114 from diffusing into the air outside the detection device 100. In some embodiments of the present utility model, the filtering unit 216 may further include a gas-permeable filter screen to prevent the contaminated liquid of the amplification reaction from filtering out.

[0064] According to an embodiment of the present utility model, the amplification reaction unit 110 may further include a first interface (not shown), wherein a first port of the first interface may communicate with the reaction chamber 114 in the amplification reaction unit 110, and a second port of the first interface may be inserted into an installation port provided on the test strip reaction unit 120 for accommodating the first interface, so that the amplification product in the reaction chamber 114 flows through the first interface and directly enters the test strip reaction unit 120 for detection, which will be further described below and will not be elaborated here.

[0065] Return to Figure 2, the test strip reaction unit 120 may include: a test strip 122 for detecting the amplification product, and a housing 124 for accommodating the test strip 122.

[0066] Regarding the test strip 122, it is, for example, used to detect the amplification product obtained from the reaction chamber 114 through the amplification reaction. In the present utility model, the test strip 112 may be a test strip generally used for biological detection, such as a colloidal gold test strip. According to an embodiment of this aspect, the test strip 122 may include a detection result presentation unit for displaying the detection result.

[0067] Regarding the housing 124, it may include a visual window 126, which is provided on the side of the housing 124 facing the amplification reaction unit 110 for displaying the detection result status of the test strip 122 accommodated in the housing 124. The visual window 126 is transparent. According to an embodiment of the present utility model, the position of the visual window 126 on the housing 124 may correspond to the presentation position of the detection result status of the test strip 122. For example, if the detection result status of the test strip 122 is presented in the middle section of the test strip 122, and the middle section of the test strip 122 coincides with the center of the housing, then the visual window 126 of the housing 124 is correspondingly provided at the center position of the side of the housing 124 facing the amplification reaction unit 110.

[0068] According to an embodiment of the present utility model, the housing 124 may be composed of an upper housing and a lower housing, which will be described in detail below in conjunction with Figure 4 Detailed description.

[0069] As Figure 4 shown, the housing 124 includes an upper housing 312 and a lower housing 314, and the upper housing 312 and the lower housing 314 may be snap-fitted to each other.

[0070] Regarding the upper housing 312, a visual window 126, at least one jack 316 for coupling with the amplification reaction unit 110, and an installation port 318 for accommodating the first interface of the amplification reaction unit 110 are provided thereon.

[0071] Regarding the lower housing 314, a sealing strip 128 may be provided at the inner edge thereof, so that when the upper housing 312 and the lower housing 314 are snap-fitted, the amplification product in the test strip reaction unit 120 is prevented from leaking through the joint of the upper housing 312 and the lower housing 314.

[0072] According to an embodiment of the present utility model, a rectangular groove is further provided inside the lower housing 314 for accommodating the test strip (for example, Figure 2 the test strip 122). And, inwardly protruding members may be provided around the rectangular groove for firmly clamping the test strip in the rectangular groove. In as Figure 4In the illustrated embodiment, the inwardly protruding member may be disposed at a position near the end of the rectangular groove and / or at a position near the middle section of the rectangular groove. It should be understood that the position of the inwardly protruding member is not limited thereto. In addition, at least one end of the rectangular groove may be convergent to limit the position of the test strip accommodated in the rectangular groove.

[0073] Returning again to Figure 2 , as Figure 2 shown, the amplification reaction unit 110 and the test strip reaction unit 120 are arranged at a preset angle and communicate via a first interface (not shown) of the amplification reaction unit 110.

[0074] According to an embodiment of the present invention, the amplification reaction unit 110 may be detachably coupled to the test strip reaction unit 120. For example, the amplification reaction unit 110 may be pluggably inserted into the test strip reaction unit 120 through at least one plug provided on the amplification reaction unit 110 and at least one jack provided on the test strip reaction unit 120.

[0075] Specifically, with reference to Figure 3 and Figure 4 , at least one plug, i.e., the first plug 218 and the second plug (not shown), may be provided on the first surface of the amplification reaction unit 110 (i.e., the side surface of the amplification reaction unit 110 facing the test strip reaction unit 120), and at least one jack corresponding to the at least one plug may be provided on the first surface of the housing 124 of the test strip reaction unit 120. For example, the upper housing 312 of the test strip reaction unit 120 is provided with a first jack and a second jack (collectively referred to as jacks 316) corresponding to the first plug 218 and the second plug (not shown) respectively. By inserting the first plug into the first jack and the second plug into the second jack, the amplification reaction unit 110 can be pluggably inserted into the test strip reaction unit 120, thereby realizing detachably coupling the amplification reaction unit 110 to the test strip reaction unit 120.

[0076] Furthermore, in some embodiments of this aspect, a plurality of different plugs may be provided on the amplification reaction unit 110, and a plurality of jacks corresponding to the plurality of plugs are correspondingly provided on the test strip reaction unit 120, and the plurality of jacks are also different from each other, so that the amplification reaction unit 110 can be more firmly coupled to the test strip reaction unit 120 while preventing the amplification reaction unit 110 from being installed in the wrong direction.

[0077] Next, how the amplification reaction unit 110 communicates with the test strip reaction unit 120 via the first interface will be described in conjunction with Figures 5A to 6B .

[0078] Figure 5A A cross-sectional view of the amplification reaction unit 110 in the horizontal direction is shown.

[0079] As Figure 5A shown, the first port of the first interface 410 can communicate with the reaction chamber 114 in the amplification reaction unit 110, and the second port of the first interface 410 can be coupled to the mounting port 318 in Figure 3 , so that the reaction chamber 114 of the amplification reaction unit 110 is communicated with the test strip reaction unit 120 via the first interface 410, facilitating the amplified product in the reaction chamber 114 to flow through the first interface 410 and contact the test strip 122. According to some embodiments of the present invention, the second port of the first interface 410 and the mounting port 318 can adopt the form of a taper joint.

[0080] As Figure 5A shown, a first channel 412 can be provided in the first interface 410, where both ends of the first channel 412 are flush with the two ports of the first interface. The first end of the first channel 412 is communicated with the reaction chamber 114, and the second end of the first channel 412 contacts the sample pad portion of the test strip 122 in the test strip detection unit 120 when the amplification reaction unit 110 is coupled to the test strip detection unit 120.

[0081] Furthermore, according to the embodiments of the present invention, the diameter of the first end of the first channel 412 is larger than that of its second end, so that the amplified product in the amplification reaction unit 110 can more easily flow into the test strip detection unit 120 along the first channel 412. In some embodiments, the diameters of the first end and the second end of the first channel 412 need to meet a predetermined size to control the flow of the amplified product in the first channel 412. For example, the diameter range of the first end of the first channel 412 can be 1 - 6 mm, so that the amplified product will not easily enter the first channel 412 during the amplification reaction; and the diameter range of the second end of the first channel 412 can be 0.8 - 5 mm, so as to facilitate the amplified product to smoothly flow out of the first channel 412 at a suitable rate.

[0082] Figure 5B shows a top view of the first surface of the amplification reaction unit 110.

[0083] As Figure 5B shown, the cross-section of the first interface 410 is rectangular, which can prevent the amplified reaction unit 110 from rotating relative to the test strip detection unit 120 after coupling, increasing the stability of the detection device 100.

[0084] As Figure 5B shown, a sealing ring 414 can also be provided on the outer side wall of the first interface 410 to ensure that the first interface 410 between the amplification reaction unit 110 and the test strip detection unit 120 is sealed, thereby preventing the amplified product from leaking when flowing through the first interface.

[0085] Figure 6A and Figure 6B show a vertical cross-section of the detection device 100 in different postures. Figure 6A show a vertical cross-section of the detection device 100 placed in the first posture. Figure 6B show a vertical cross-section of the detection device 100 placed in the second posture.

[0086] As Figure 6A shown, the amplification reaction unit 110 is further provided with a reagent storage chamber 512 for storing reagents for the amplification reaction. When a sample is added to the reaction chamber 114 via the sample inlet 112, the sample can directly undergo an amplification reaction with the reagents in the reagent storage chamber 512.

[0087] According to an embodiment of the present invention, the amount of the added sample needs to satisfy that the liquid level in the reaction chamber 114 after adding the sample cannot be higher than the lower edge of the first end of the first interface 410, so as to ensure that when a sample is added to the amplification reaction unit 110 and an amplification reaction is carried out in the amplification reaction unit 110, the reagents (such as reaction reagents, samples, etc.) and / or reaction products (such as aerosols, amplification products) in the amplification reaction unit 110 will not enter the first interface 410 through the first end of the first interface 410.

[0088] As Figure 6B shown, after the detection device 100 is placed in the second posture, the amplification products in the reaction chamber 114 can flow into the test strip reaction unit 120 through the first interface 410 due to the action of gravity and contact the test strip 122 therein. As described above, the visual window 126 corresponds to the position where the detection result state of the test strip 122 is presented and is configured to display the detection result state of the test strip 122. Therefore, the detection result state of the test strip 122 can be observed through the visual window 126 to determine the detection result.

[0089] In summary, according to the detection device 100 provided by the embodiment of the present invention, by setting the amplification reaction unit 110 and the test strip reaction unit 120 at a preset angle and connecting them through the first interface 410, when the detection device 100 rotates a predetermined angle, for example, by tapping a predetermined number of times, the amplification products generated in the reaction chamber 140 of the amplification reaction unit 110 can directly flow into the test strip reaction unit 120 through the first interface 410 (such as the first channel 412 provided in the first interface 410) and contact the test strip 122 in the test strip reaction unit 120 to realize the detection of the amplification products, significantly reducing the pollution risk, and at the same time having simple operation and high detection efficiency.

[0090] According to the present utility model, in some embodiments, in order to enable the amplification products flowing into the test strip reaction unit 120 to better contact the test strip 122, a microchannel may be further provided inside the housing 124 of the test strip reaction unit 120. The liquid inlet end of the microchannel is connected to the first interface 410, and the liquid outlet end is connected to the first end of the test strip 122, so that the amplification products can flow along the microchannel to the end of the test strip 122 where the sample pad part is provided and directly contact the sample pad part of the test strip 122, improving the detection efficiency.

[0091] For example, in some examples, the microchannel may be provided at a position corresponding to the installation opening 318 of the upper housing 312 inside the lower housing 314 of the test strip reaction unit 120. In these examples, since the first port of the first interface 410 of the amplification reaction unit 110 is communicated with the reaction chamber 114 in the amplification reaction unit 110, by making the second port of the first interface 410 communicate with the liquid inlet end of the microchannel in the test strip reaction unit 120, the amplification products obtained in the reaction chamber 114 can flow through the first interface 410 and into the microchannel, so as to contact the test strip 122 along the microchannel.

[0092] According to the present utility model, in still other embodiments, the amplification reaction unit of the detection device provided by the present utility model may include a plurality of reaction chambers for simultaneously performing amplification reactions on multiple samples. Similarly, in still other embodiments, the test strip reaction unit of the detection device provided by the present utility model may include a plurality of test strips for simultaneously detecting the amplification products. In addition, according to the embodiments of the present utility model, the number of reaction chambers included in the amplification reaction unit and the number of test strips included in the test strip reaction unit may be the same or different, and the present utility model does not limit this. Figures 7 to 10 The schematic diagram of the detection device according to other embodiments of the present utility model is shown.

[0093] Figure 7 The schematic diagram of the detection device 700 according to the second embodiment of the present utility model is shown.

[0094] As Figure 7 shown, the detection device 700 includes an amplification reaction unit 710 and a test strip reaction unit 720, wherein the amplification reaction unit 710 and the test strip reaction unit 720 are arranged at a preset angle (such as 90°) and are communicated via a first interface (not shown).

[0095] As Figure 7 shown, the amplification reaction unit 710 includes three reaction chambers. Correspondingly, each reaction chamber is provided with a corresponding sample inlet above it.

[0096] In this embodiment, the same or different samples can be added to the three sample inlets respectively. Moreover, the reagent storage chambers in each of the three reaction chambers can store the same or different reagents for performing amplification reactions with the samples added thereto, and the present utility model places no restrictions thereon.

[0097] Thereby, different amplification products obtained in multiple reaction chambers can be mixed and then enter the first interface, and flow into the test strip reaction unit 720 via the first interface to contact the test strip in the test strip reaction unit 720, so as to realize the detection of the mixture of multiple amplification products.

[0098] Figure 8 The schematic diagram of the detection device 800 according to the third embodiment of the present utility model is shown.

[0099] As Figure 8 shown, the detection device 800 includes an amplification reaction unit 810 and a test strip reaction unit 820. Among them, the amplification reaction unit 810 and the test strip reaction unit 820 are vertically arranged and communicated via a first interface (not shown).

[0100] As Figure 8 shown, the test strip reaction unit 820 includes three test strips. Correspondingly, three visual windows are provided on the upper housing of the test strip reaction unit 820, and each visual window corresponds to one of the three test strips for displaying the detection result status of the test strip.

[0101] As Figure 8 shown, a microchannel 832 is further provided inside the lower housing of the test strip reaction unit 820. The microchannel 832 has an inlet end and three outlet ends. Among them, the inlet end of the microchannel 832 is communicated with the second port of the first interface, and each outlet end of the microchannel 832 is connected to the first end of one of the three test strips.

[0102] In this embodiment, the amplification products generated in the amplification reaction unit 810 can flow into the microchannel 832 via the first interface, and then can contact the three test strips respectively via its three outlet ends along the microchannel 832, so as to realize multiple detections of the same amplification product simultaneously.

[0103] Figure 9 The schematic diagram of the detection device 900 according to the fourth embodiment of the present utility model is shown.

[0104] As Figure 9 shown, the detection device 900 includes an amplification reaction unit 910 and a test strip reaction unit 920. Among them, the amplification reaction unit 910 and the test strip reaction unit 920 are vertically arranged and communicated via a plurality of interfaces (not shown).

[0105] AsFigure 9 As shown, the amplification reaction unit 910 includes three reaction chambers. Accordingly, a respective sample inlet is provided above each reaction chamber. The test strip reaction unit 920 includes three test strips, and three visual windows are correspondingly provided on the upper housing of the test strip reaction unit 920, with each visual window corresponding to one of the three test strips for displaying the detection result status of the test strip.

[0106] As Figure 9 shown, the amplification reaction unit 910 and the test strip reaction unit 920 are connected through three first interfaces. That is to say, the amplification products in each reaction chamber of the amplification reaction unit 910 can contact one of the test strips in the test strip reaction unit 920 through a different first interface.

[0107] In this embodiment, by making the number of reaction chambers included in the amplification reaction unit the same as the number of test strips included in the test strip reaction unit, and making each reaction chamber correspond to one of the multiple test strips, each test strip can detect the amplification products obtained in the reaction chamber corresponding to it, so as to realize the detection of the amplification products generated in different reaction chambers simultaneously, obtain the detection results of the amplification products generated in each reaction chamber, and improve the detection efficiency.

[0108] Furthermore, according to the embodiments of the present invention, the amplification reaction unit including multiple reaction chambers can be discrete or integrally formed. Taking Figure 9 the amplification reaction unit 910 shown as an example, the amplification reaction unit 910 is integrally formed. That is to say, the three reaction chambers of the amplification reaction unit 910 are integrated into one component to serve as the amplification reaction unit 910 of the detection device 900. In some other embodiments, the amplification reaction unit 910 can be discrete. That is to say, the three reaction chambers of the amplification reaction unit 910 are separately arranged on, for example, three different components, and each component is provided with an interface for allowing the amplification products to flow into the test strip reaction unit 920, so that the amplification products in the reaction chamber on the current component can flow into the test strip reaction unit 920 through this interface for detection.

[0109] Figure 10 FIG. shows a schematic diagram of a detection device 1000 according to a fifth embodiment of the present invention.

[0110] As Figure 10 shown, the detection device 1000 includes an amplification reaction unit 1010 and a test strip reaction unit 1020, wherein the amplification reaction unit 1010 and the test strip reaction unit 1020 are vertically arranged and connected through a first interface (not shown).

[0111] As Figure 10As shown, the amplification reaction unit 1010 includes three reaction chambers. Correspondingly, each reaction chamber is provided with a respective corresponding sample inlet above it. The test strip reaction unit 1020 includes three test strips, and three visual windows are correspondingly provided on the upper housing of the test strip reaction unit 920, and each visual window corresponds to one of the three test strips for displaying the detection result status of the test strip.

[0112] Different from Figure 9 the detection device 900 described above, the amplification products obtained in the three reaction chambers of the detection device 1000 are mixed and then detected. That is to say, the detection object of the detection device 1000 is the amplified product after mixing. To achieve this effect, as Figure 10 shown, a microchannel 1032 is provided inside the lower housing of the detection device 1000. Similar to Figure 8 the microchannel 832, Figure 10 the microchannel 1032 has an inlet end and three outlet ends. Among them, the inlet end of the microchannel 1032 is connected to the second port of the first interface, and each outlet end of the microchannel 1032 is connected to the first end of one of the three test strips.

[0113] In this embodiment, the amplification products in the multiple reaction chambers of the amplification reaction unit 1010 are mixed and then flow into the first interface and then into the microchannel 1032 to contact each of the multiple test strips in the test strip reaction unit 1020. Specifically, the different amplification products obtained in the three reaction chambers of the amplification reaction unit 1010 are mixed and enter the first interface, and then flow into the microchannel 1032 through the first interface, and then can contact the three test strips respectively through its three outlet ends along the microchannel 1032, so as to realize multiple detections on the mixed solution of multiple amplification products at the same time.

[0114] The above has described the embodiments of the present invention. The above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes are obvious to those of ordinary skill in the art in the technical field without departing from the scope and spirit of the described embodiments. The selection of the terms used herein is intended to best explain the principles of the embodiments, practical applications, or improvements to the technology in the market, or to enable other ordinary technical personnel in the technical field to understand the disclosed embodiments.

[0115] The above are only optional embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A detection device, characterized in that: include: An amplification reaction unit, comprising at least a sample inlet, a reaction chamber and a sealing cover, wherein the sample inlet is configured to receive a sample provided to the amplification reaction unit, the reaction chamber is configured to provide a space for an amplification reaction, and the sealing cover is configured to seal the sample inlet; as well as The test paper reaction unit comprises at least a test paper strip for detecting an amplification product and a housing for accommodating the test paper strip, wherein the test paper strip is used to detect an amplification product obtained from the reaction chamber through an amplification reaction. Wherein, the amplification reaction unit and the test strip reaction unit are arranged at a preset angle and are connected via a first interface of the amplification reaction unit.

2. The detection device according to claim 1, characterized in that: The first interface is configured such that when the detection device is rotated by a predetermined angle and knocked a predetermined number of times, the amplification product obtained in the reaction chamber flows through the first interface to contact the test strip.

3. The detection device according to claim 1, characterized in that: The reaction chamber is provided with a first coupling portion for coupling with a second coupling portion provided on the sealing cover, so as to fix the sealing cover.

4. The detection device according to claim 1, characterized in that: The amplification reaction unit further includes: an anti-contamination component, which is arranged at the sample inlet to prevent the reagents and / or samples in the amplification reaction unit from being contaminated.

5. The detection device according to claim 1, characterized in that: The amplification reaction unit further includes: a filtering unit, wherein the filtering unit is provided with a filter sheet to prevent aerosol generated by the sample in the reaction chamber from escaping.

6. The detection device according to claim 1, characterized in that: The amplification reaction unit is detachably coupled to the test paper reaction unit.

7. The detection device according to claim 6, characterized in that: The first surface of the amplification reaction unit is provided with a first plug-in and a second plug-in, the first surface of the shell of the test paper reaction unit is provided with a first jack corresponding to the first plug-in and a second jack corresponding to the second plug-in, and the amplification reaction unit is detachably coupled to the test paper reaction unit via the first plug-in and the second plug-in.

8. The detection device according to claim 1, characterized in that: A visual window is provided on the first surface of the shell of the test paper reaction unit, and the visual window is configured to display the detection result status of the test paper strip.

9. The detection device according to any one of claims 1 or 8, characterized in that: The shell of the test paper reaction unit includes an upper shell and a lower shell that are interlocked. Wherein, a sealing strip is arranged at the inner edge of the lower shell body to prevent the amplification product in the test paper reaction unit from leaking out.

10. The detection device according to claim 1, characterized in that: The amplification reaction unit includes a plurality of reaction chambers.

11. The detection device according to claim 10, characterized in that: The amplification reaction unit including a plurality of reaction chambers is discrete or integrally formed.

12. The detection device according to claim 10, characterized in that: The test paper reaction unit includes a plurality of test paper strips.

13. The detection device according to claim 12, characterized in that: The number of the plurality of reaction chambers is the same as the number of the plurality of test strips, and each reaction chamber corresponds to one of the plurality of test strips, so that each test strip detects the amplification product obtained in the reaction chamber corresponding thereto.

14. The detection device according to claim 10, characterized in that: The shell of the test paper reaction unit is provided with a plurality of visible windows, each of which corresponds to one of the plurality of test strips and is used to display the detection result status of the test strip.

15. The detection device according to claim 1, characterized in that: A microchannel is arranged inside the shell of the test paper reaction unit, the liquid inlet end of the microchannel is connected to the first interface, and the liquid outlet end of the microchannel is connected to the first end of the test paper strip.

16. The detection device according to claim 15, characterized in that: The first port of the first interface is connected to the reaction chamber in the amplification reaction unit, and the second port of the first interface is connected to the liquid inlet end of the microfluidic channel in the test paper reaction unit, so that the amplification product obtained in the reaction chamber flows through the first interface and flows into the microfluidic channel, thereby contacting the test paper strip.

17. The detection device according to claim 15, characterized in that: The test paper reaction unit includes a plurality of test paper strips, and the microfluidic channel includes a plurality of liquid outlet ends, wherein each liquid outlet end is connected to a first end of a test paper strip among the plurality of test paper strips.

18. The detection device according to claim 17, characterized in that: The amplification reaction unit includes a plurality of reaction chambers, wherein the amplification products in the plurality of reaction chambers flow into the first interface after being mixed and then flow into the microfluidic channel to contact each of the plurality of test strips.

19. The detection device according to claim 1, characterized in that: The preset angle is any angle between 70° and 110°.

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