Cell liquid changing device for culture bottle

Abstract

The utility model discloses a cell liquid changing device for a culture bottle. The cell liquid changing device comprises an inlet and outlet pipeline, a control center, a communicating pipeline and a liquid storage bottle, the control center is a controller, a fixed supporting structure is arranged below the controller, the inlet and outlet pipeline comprises a liquid discharge pipe and a liquid inlet pipe, the liquid discharge pipe and the liquid inlet pipe are arranged on one side of the controller side by side, the number of the liquid storage bottles is three, and the three liquid storage bottles are respectively a waste liquid bottle, a cleaning liquid bottle and a culture liquid bottle. The waste liquid bottle, the cleaning liquid bottle and the culture liquid bottle are connected with the controller through communicating pipelines, the communicating pipelines comprise a culture liquid pipeline, a cleaning liquid pipeline and a waste liquid pipeline, a first liquid pump and a second liquid pump are arranged in the controller, one end of the first liquid pump is connected with a liquid inlet pipe, the other end of the first liquid pump is connected with the culture liquid pipeline and the cleaning liquid pipeline, and one end of the second liquid pump is connected with a liquid discharging pipe. According to the cell liquid changing device for the culture bottle, provided by the utility model, the liquid changing efficiency is conveniently improved, and the productivity is liberated.

Description

Technical Field

[0001] The utility model relates to the field of laboratory technical equipment, and more specifically to a cell liquid changing device for culture bottles. Background Art

[0002] Cell culture is a commonly used technique in biological and medical research. Among them, changing the liquid for adherent cells is a basic experimental operation. Most of the existing methods for changing the liquid of adherent cells adopt manual operation. During the process of pouring the waste liquid from the culture bottle, it is very easy to cause the loss of adherent cells. Moreover, when adding new liquid, due to the manual addition method, it is difficult to ensure the addition amount and uniformity, and it is also very easy to damage the adherent cells. In addition, during the large-scale production process in "cell factories" and enterprises, the workload of cell liquid changing is very large, and the operators need to frequently repeat the pipetting operation in the ultra-clean bench. Therefore, it is very necessary to establish a safe and fast liquid changing device to liberate productivity, standardize experimental operations, and improve production efficiency. Content of the Utility Model

[0003] The technical problem to be solved by the utility model is that most of the existing methods for changing the liquid of adherent cells adopt manual operation. During the process of pouring the waste liquid from the culture bottle, it is very easy to cause the loss of adherent cells. Moreover, when adding new liquid, due to the manual addition method, it is difficult to ensure the addition amount and uniformity, and it is also very easy to damage the adherent cells.

[0004] To solve the above technical problems, the utility model adopts the following technical means:

[0005] A cell liquid changing device for culture bottles includes an inlet and outlet pipeline, a control center, a connecting pipeline, and a liquid storage bottle; the control center is a controller, and a fixed support structure is arranged below the controller. The inlet and outlet pipeline includes a drain pipe and a liquid inlet pipe, and the drain pipe and the liquid inlet pipe are arranged side by side on one side of the control. The number of liquid storage bottles is three, which are a waste liquid bottle, a cleaning liquid bottle, and a culture medium bottle respectively. The waste liquid bottle, the cleaning liquid bottle, and the culture medium bottle are connected to the controller through the connecting pipeline. The connecting pipeline includes a culture medium pipeline, a cleaning liquid pipeline, and a waste liquid pipeline. A liquid pump one and a liquid pump two are arranged in the controller. One end of the liquid pump one is connected to the liquid inlet pipe, and the other end of the liquid pump one is connected to the culture medium pipeline and the cleaning liquid pipeline. One end of the liquid pump two is connected to the drain pipe, and the other end of the liquid pump two is connected to the waste liquid pipeline. A switch structure is arranged on the controller. The utility model provides a cell liquid changing device for culture bottles, which is convenient for improving the liquid changing efficiency and liberating productivity.

[0006] As a preference, a further technical solution of the utility model is:

[0007] The fixed support structure includes a base and a support plate. The lower end of the support plate is connected to the base, and the upper end of the support plate is connected below the controller.

[0008] The connecting pipeline between the described controller and the waste liquid bottle is a waste liquid pipeline, the connecting pipeline between the controller and the cleaning liquid bottle is a cleaning liquid pipeline, and the connecting pipeline between the controller and the culture medium bottle is a culture medium pipeline.

[0009] The described switch structure is a control button and pipe clamps. The pipe clamps include pipe clamp one, pipe clamp two, and pipe clamp three. Pipe clamp one is arranged at the top of the culture medium pipeline, pipe clamp two is arranged at the top of the cleaning liquid pipeline, and pipe clamp three is arranged at the top of the waste liquid pipeline.

[0010] The described control buttons include control button a, control button b, and control button c. Control button a is arranged above pipe clamp one, control button b is arranged above pipe clamp two, and control button c is arranged above pipe clamp three. The control buttons control the opening and closing of the pipe clamps.

[0011] The nozzle of the described inlet pipe is in a downward-bending shape.

[0012] A telescopic pipe is provided at the nozzle of the described drain pipe. The telescopic pipe can be retracted at the nozzle of the drain pipe or extended out of the nozzle of the drain pipe.

[0013] A groove body is provided on the described base. The groove body is a placement position for the culture bottle. The drain pipe and the inlet pipe are arranged above the placement position of the culture bottle.

[0014] Scale lines and labels are provided on the described culture medium bottle.

[0015] A cell liquid-changing device for a culture bottle of the present utility model can replace the traditional pure manual cell liquid-changing method, improve work efficiency, avoid repetitive operations, and at the same time, the elbow design of the inlet pipe can prevent the newly added liquid in the culture bottle from generating bubbles and affecting the growth and reproduction of cells. Description of the Drawings

[0016] Figure 1 It is a schematic diagram of the cell liquid-changing device of the present utility model.

[0017] Figure 2 It is a schematic diagram of the internal structure of the cell liquid-changing device of the present utility model.

[0018] Description of the Reference Numerals:

[0019] 01 Drain pipe; 02 Inlet pipe; 03 Culture bottle placement; 04 Base; 05 Support plate; 06 Waste liquid bottle; 07 Cleaning liquid bottle; 08 Culture liquid bottle; 09 Scale line; 10 Label; 11 Controller; 12 Control button a; 13 Control button b; 14 Control button c; 15 Culture liquid pipeline; 16 Cleaning liquid pipeline; 17 Waste liquid pipeline;

[0020] 101 telescopic tube; 11 controller; 111 liquid pump one; 112 liquid pump two; 113 pipe clamp one; 114 pipe clamp two; 114 pipe clamp three. DETAILED DESCRIPTION

[0021] The present invention will be further described below in conjunction with the embodiments. Specific embodiments

[0022] See also Figure 1 It can be seen that the utility model is a cell fluid exchange device for a culture bottle, comprising a bottom plate, a controller, and a liquid storage bottle; the liquid storage bottles are three in total, namely a waste liquid bottle, a cleaning liquid bottle and a culture liquid bottle, which are connected to the controller through corresponding waste liquid pipes, cleaning liquid pipes, and culture liquid pipes; wherein the cleaning liquid pipe and the culture liquid pipe are combined into a liquid inlet pipe in the controller, and the flow of liquid in the liquid inlet pipe is controlled by a liquid pump 1; the waste liquid pipe enters the controller and is connected to the discharge pipe and the flow of liquid is controlled by a liquid pump 2; there are control buttons a, b, and c in the controller, which respectively control tube clamps one, two, and three; the tube clamps one, two, and three respectively control the opening and closing of the three pipes; the pipe mouth of the liquid inlet pipe is curved, which is conducive to the liquid flowing down from the side wall of the container and preventing bubbles from being generated when adding liquid. Specific embodiments

[0023] See also Figure 2 It can be seen that the utility model is a cell fluid exchange device for a culture bottle. When in use, the culture bottle is placed at the culture bottle placement position 03, and the control button c14 is first turned on. The drainage pipe 01 is linked to the internal telescopic tube 101 to extend to the bottom of the culture bottle to suck out the culture fluid in the culture. The sucked culture fluid is discharged into the waste liquid bottle 06 through the drainage pipe 01 and the waste liquid pipe 17. The culture fluid pipe 15 and the cleaning fluid pipe 16 are respectively controlled by the control buttons a12 and b13 to send different types of liquids to the culture bottle through the liquid inlet pipe 02.

[0024] Since the above is only a specific implementation method of the present utility model, the protection of the present utility model is not limited thereto, and any equivalent changes or substitutions of the technical features of the technical solution that can be thought of by any technician in this technical field are included in the protection scope of the present utility model.

Claims

1. A cell liquid replacement device for a culture bottle, comprising an inlet and outlet pipeline, a control center, a connecting pipeline and a liquid storage bottle; characterized in that: The control center is a controller, and a fixed supporting structure is provided under the controller. The inlet and outlet pipelines include a discharge pipe and an inlet pipe, and the discharge pipe and the inlet pipe are arranged side by side on one side of the controller. There are three liquid storage bottles, and the three liquid storage bottles are respectively a waste liquid bottle, a cleaning liquid bottle and a culture liquid bottle. The waste liquid bottle, the cleaning liquid bottle and the culture liquid bottle are connected to the controller through a connecting pipeline. The connecting pipeline includes a culture liquid pipeline, a cleaning liquid pipeline and a waste liquid pipeline. A liquid pump 1 and a liquid pump 2 are provided in the controller. One end of the liquid pump 1 is connected to the inlet pipe, and the other end of the liquid pump 1 is connected to the culture liquid pipeline and the cleaning liquid pipeline. One end of the liquid pump 2 is connected to the discharge pipe, and the other end of the liquid pump 2 is connected to the waste liquid pipeline. A switch structure is provided on the controller.

2. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: The fixed support structure comprises a base and a support plate, the lower end of the support plate is connected to the base, and the upper end of the support plate is connected below the controller.

3. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: The connecting pipeline between the controller and the waste liquid bottle is a waste liquid pipeline, the connecting pipeline between the controller and the cleaning liquid bottle is a cleaning liquid pipeline, and the connecting pipeline between the controller and the culture liquid bottle is a culture liquid pipeline.

4. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: The switch structure is a control button and a tube clamp, and the tube clamp includes tube clamp one, tube clamp two and tube clamp three. Tube clamp one is arranged on the top of the culture fluid pipeline, tube clamp two is arranged on the top of the cleaning fluid pipeline, and tube clamp three is arranged on the top of the waste fluid pipeline.

5. The cell liquid replacement device for a culture bottle according to claim 4, characterized in that: The control buttons include control button a, control button b and control button c. The control button a is arranged above the tube clamp one, the control button b is arranged above the tube clamp two, and the control button c is arranged above the tube clamp three. The control buttons control the opening and closing of the tube clamps.

6. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: The pipe opening of the liquid inlet pipe is in a downward curved shape.

7. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: A telescopic tube is arranged at the pipe opening of the liquid discharge pipe, and the telescopic tube can be retracted at the pipe opening of the liquid discharge pipe or extended out of the pipe opening of the liquid discharge pipe.

8. The cell liquid replacement device for a culture bottle according to claim 2, characterized in that: A trough body is provided on the base, the trough body is a position for placing the culture bottle, and the liquid discharge pipe and the liquid inlet pipe are arranged above the position for placing the culture bottle.

9. The cell liquid replacement device for a culture bottle according to claim 1, characterized in that: The culture fluid bottle is provided with scale lines and labels.

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