Sample adding structure and full-automatic immunofluorescence staining instrument

Abstract

The utility model relates to a sample adding structure and a full-automatic immunofluorescence staining instrument, and the sample adding structure comprises a first driving assembly which comprises a first guide rail; the cantilever beam is movably arranged on the first guide rail in the first direction, the cantilever beam comprises a first end and a second end which are opposite in the second direction, and the first end is located on the first guide rail; the sample adding assembly comprises a sample adding needle, and the sample adding needle is mounted on the part, extending out of the first guide rail, of the cantilever beam; the second driving assembly comprises a second driving piece, and the second driving piece is arranged at the first end; the third driving assembly comprises a third driving part, the third driving part is arranged at the first end, and the weight of the second driving part and the third driving part can act on the first guide rail through the cantilever beam, so that the bending moment borne by the cantilever beam is reduced, the weight of the cantilever beam is reduced, and the effect of simplifying the sample adding structure is achieved; meanwhile, the second driving part and the third driving part are arranged at the first end of the cantilever beam, so that the second driving part and the third driving part can be conveniently mounted and debugged.

Description

Technical Field

[0001] This application relates to the technical field of medical testing equipment, and particularly to a sample adding structure and a fully automatic immunofluorescence staining instrument. Background Art

[0002] Immunofluorescence staining technology is a protein and other molecular detection technology developed based on immunology, combined with biochemical technology and microscopy technology. Due to its characteristics such as strong specificity, high sensitivity, fast speed, and easy observation, it is widely used in fields such as cell biology, microbiology, and clinical diagnosis.

[0003] The fully automatic immunofluorescence staining instrument realizes precise, fast, and reliable sample adding and sampling actions on samples through the sample adding structure, thereby enabling the automation of immunohistochemical staining. Existing fully automatic immunofluorescence staining usually adopts the method of a cantilever beam cooperating with a Z-axis assembly. The cantilever beam can move in the X direction. A slider that can move in the Y direction is arranged on the cantilever beam, and the Z-axis assembly is arranged on the slider to move on the cantilever beam along with the slider.

[0004] Among them, the Z-axis assembly usually includes a Z-axis driving component and a sampling needle, so as to drive the sampling needle to move on the Z-axis through the Z-axis driving component. When the slider moves on the cantilever beam, the Z-axis driving component and the sampling needle move on the cantilever beam together. Since the Z-axis driving component includes components such as a motor, the weight of the Z-axis driving component is relatively large. When the slider drives the Z-axis assembly close to the overhanging end of the cantilever beam, the relatively heavy Z-axis driving component will cause the cantilever beam to bear a large bending moment. Therefore, it is necessary to strengthen the structure of the cantilever beam to ensure the stability of the operation of the Z-axis assembly, and finally the quality and cost of the overall equipment will also increase accordingly. Summary of the Invention

[0005] Based on this, in view of the problem that the cantilever beam bears a large bending moment, it is necessary to provide a sample adding structure and a fully automatic immunofluorescence staining instrument.

[0006] A sample adding structure includes:

[0007] A first driving component, including a first guide rail extending longitudinally along a first direction;

[0008] A cantilever beam extending longitudinally along a second direction and including a first end and a second end that are oppositely arranged. The first end is movably arranged on the first guide rail along the first direction, and the second end extends out of the first guide rail;

[0009] A sample adding component, including a sampling needle, and the sampling needle is movably installed on the part of the cantilever beam extending out of the first guide rail along both the second direction and a third direction;

[0010] The second driving assembly includes a second driving member, which is disposed at the first end and is configured to provide power for the movement of the sampling needle along the second direction;

[0011] The third driving assembly includes a third driving member, which is disposed at the first end and is configured to provide power for the movement of the sampling needle along the third direction;

[0012] Wherein, the first direction, the second direction and the third direction intersect pairwise and are not coplanar.

[0013] In one embodiment, the third driving assembly further includes a spline shaft, which is disposed on the cantilever beam and extends longitudinally along the second direction. The third driving member is in transmission connection with the spline shaft and is configured to drive the spline shaft to rotate about its own axis;

[0014] The sampling assembly further includes a movable member, which is movably mounted on the cantilever beam along the second direction. The second driving member is in transmission connection with the movable member and is configured to provide power for the movement of the movable member along the second direction;

[0015] The movable member is provided with a movable portion that can move along the third direction. A rack extending longitudinally along the third direction is provided on the movable portion. The rack meshes with the external spline of the spline shaft, and the sampling needle is disposed on the movable portion.

[0016] In one embodiment, the second driving assembly further includes a driving wheel and a synchronous belt. There are two driving wheels. The two driving wheels are spaced apart along the second direction on the cantilever beam. The synchronous belt is wound between the two driving wheels. The movable member is connected to the synchronous belt. The second driving member is in transmission connection with the driving wheel close to the first end and is configured to drive the driving wheel to rotate.

[0017] In one embodiment, a second guide rail extending longitudinally along the second direction is provided on the cantilever beam. The sampling assembly includes multiple groups. The movable members of each group of sampling assemblies are movably mounted on the second guide rail;

[0018] The second driving assembly includes multiple groups. The movable members of each group of sampling assemblies are connected to the synchronous belt of one group of the second driving assemblies;

[0019] And / or, the third driving assembly includes multiple groups. The racks of each group of sampling assemblies mesh with the spline shafts of one group of the third driving assemblies.

[0020] In one embodiment, the sample adding assembly further includes a mounting member and an adjusting member. The sample adding needle is mounted on the mounting member. The mounting member is movably mounted on the adjusting member along the first direction, and the adjusting member is mounted on the movable portion.

[0021] In one embodiment, a chute extending longitudinally along the first direction is provided on one of the mounting member and the adjusting member, and a boss is protrudingly provided on the other. The boss and the chute cooperate with each other.

[0022] The sample adding assembly further includes an adjusting nut. An adjusting platform is protrudingly provided on the adjusting member towards the mounting member. A threaded hole extending longitudinally along the first direction is formed in the adjusting platform. The adjusting nut is threadedly connected to the threaded hole, and during the movement of the adjusting nut in the threaded hole, the adjusting nut can abut against the mounting member.

[0023] In one embodiment, the sample adding assembly further includes a retaining piece and an elastic member. The elastic member elastically connects the retaining piece and the mounting member and can elastically deform along the third direction.

[0024] The sample adding needle is movably mounted on the mounting member along the third direction and is connected to the retaining piece.

[0025] In one embodiment, the sample adding assembly further includes an emergency stop sensor. The emergency stop sensor is provided on the mounting member and is communicatively connected to the third driving member.

[0026] A triggering portion is provided on the retaining piece. During the movement of the retaining piece along the third direction relative to the mounting member, the triggering portion can trigger the emergency stop sensor.

[0027] In one embodiment, the sample adding assembly further includes a liquid level detecting member. The liquid level detecting member is provided on the sample adding needle.

[0028] A full-automatic immunofluorescence staining instrument includes the sample adding structure as described in any one of the above.

[0029] In the above sample adding structure, the second driving member and the third driving member are both provided at the first end of the cantilever beam. Since the first end of the cantilever beam is provided on the first guide rail, the weights of the second driving member and the third driving member can act on the first guide rail through the cantilever beam, thereby reducing the bending moment borne by the cantilever beam, facilitating reducing the weight of the cantilever beam, achieving the effect of streamlining the sample adding structure, and at the same time, arranging the second driving member and the third driving member at the first end of the cantilever beam can also facilitate the installation and debugging of the second driving member and the third driving member. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1This is a schematic structural diagram of a sample adding structure in some embodiments of the present application.

[0031] Figure 2 is Figure 1 a schematic structural diagram of a sample adding component in an embodiment.

[0032] Figure 3 is Figure 2 a schematic structural diagram of a mounting member and an adjusting member of a sample adding component in an embodiment.

[0033] Explanation of reference numerals:

[0034] The first driving assembly 10; the first guide rail 11; the first driving motor 12;

[0035] The cantilever beam 20; the first end 21; the second end 22;

[0036] The sample adding component 30; the sample adding needle 31; the movable part 32; the movable portion 33; the rack 34; the mounting member 35; the adjusting member 36; the chute 361; the boss 362; the adjusting nut 363; the adjusting table 364; the retaining piece 37; the elastic member 371; the emergency stop sensor 372; the triggering portion 373; the liquid level detecting member 38; the liquid level detecting plate 381;

[0037] The second driving assembly 40; the second driving member 41; the driving wheel 42; the synchronous belt 43; the second guide rail 44;

[0038] The third driving assembly 50; the third driving member 51; the spline shaft 52;

[0039] The first direction X, the second direction Y, and the third direction Z. Detailed implementation manners

[0040] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed implementation manners of the present application with reference to the accompanying drawings. Many specific details are set forth in the following description to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0041] In the description of the present application, it should be understood that if there are terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., the orientation or positional relationship indicated by these terms is based on the orientation or positional relationship shown in the drawings. These are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present application.

[0042] In addition, if there are terms such as "first" and "second", these terms are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present application, if there is a term "plurality", the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise specifically and clearly defined.

[0043] In the present application, unless otherwise clearly specified and limited, if there are terms such as "mounted", "connected", "connected to", "fixed", etc., these terms should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements or the interaction relationship between two elements, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0044] In the present application, unless otherwise clearly specified and limited, if there is a description such as a first feature being "on" or "under" a second feature, the meaning may be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may mean that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature is at a higher horizontal level than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature is at a lower horizontal level than the second feature.

[0045] It should be noted that if an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or there may be an intermediate element. If an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this application are only for illustrative purposes and do not represent the only implementation.

[0046] Referring to Figure 1 and Figure 2 A sample adding structure provided by an embodiment of the present application includes a cantilever beam 20, a sample adding assembly 30, as well as a first driving assembly 10, a second driving assembly 40 and a third driving assembly 50. The sample adding assembly 30 includes a sample adding needle 31 disposed on the cantilever beam 20. The sample adding needle 31 is used to extract the liquid in the reagent container or release the liquid into the corresponding reagent container. The first driving assembly 10 is used to enable the cantilever beam 20 to move in the first direction X, so that the sample adding needle 31 can move along the first direction X. The second driving assembly 40 is used to enable the sample adding needle 31 to move along the second direction Y. The third driving assembly 50 is used to enable the sample adding needle 31 to move along the third direction Z. The first direction X, the second direction Y and the third direction Z intersect pairwise and are not coplanar. The first driving assembly 10, the second driving assembly 40 and the third driving assembly 50 cooperate to enable the sample adding needle 31 to achieve movement in any direction.

[0047] Specifically, the first driving assembly 10 includes a first guide rail 11 extending longitudinally along the first direction X, two transmission synchronous belts 43 and a first driving motor 12. The cantilever beam 20 is movably disposed on the first guide rail 11 along the first direction X, and drives the two transmission synchronous belts 43 through a driving member to drive the cantilever beam 20 to move in the first direction X. In other embodiments, the movement mode of the cantilever beam 20 on the first guide rail 11 can be through a linear motor module or other means.

[0048] Among them, the cantilever beam 20 extends longitudinally along the second direction Y, and includes a first end 21 and a second end 22 opposite to each other in the second direction Y. The first end 21 is movably disposed on the first guide rail 11 along the first direction X, and the second end 22 extends out of the guide rail. The sample adding needle 31 of the sample adding assembly 30 is disposed on the part of the cantilever beam 20 extending out of the first guide rail 11, so that the sample adding needle 31 will not be affected by the first guide rail 11 in the third direction Z.

[0049] Under the action of the second driving component 40 and the third driving component 50, the sampling needle 31 can move relative to the cantilever beam 20 along the second direction Y and the third direction Z. In order to drive the movement of the sampling needle 31, the second driving component 40 includes a second driving member 41, and the second driving member 41 is used to provide power for the movement of the sampling needle 31 along the second direction Y. The third driving component 50 includes a third driving member 51, and the third driving member 51 is used to provide power for the movement of the sampling needle 31 along the third direction Z. The second driving member 41 and the third driving member 51 can be structures such as a driving motor and an air pump motor that can output power.

[0050] Wherein, both the second driving member 41 and the third driving member 51 are arranged on the first end 21 of the cantilever beam 20. Since the first end 21 of the cantilever beam 20 is arranged on the first guide rail 11, the weights of the second driving member 41 and the third driving member 51 can act on the first guide rail 11 through the cantilever beam 20, thereby reducing the bending moment borne by the cantilever beam 20, which is beneficial to reducing the weight of the cantilever beam 20 and achieving the effect of streamlining the sampling structure. At the same time, arranging the second driving member 41 and the third driving member 51 on the first end 21 of the cantilever beam 20 is also convenient for the installation and debugging of the second driving member 41 and the third driving member 51.

[0051] In some embodiments of the present application, the third driving component 50 further includes a spline shaft 52. The spline shaft 52 is arranged on the cantilever beam 20 and extends longitudinally along the second direction Y. The third driving member 51 is in transmission connection with the spline shaft 52 and is used to drive the spline shaft 52 to rotate around its own axis. The sampling component 30 further includes a movable member 32. The movable member 32 is movably installed on the cantilever beam 20 along the second direction Y. The second driving member 41 is in transmission connection with the movable member 32 and is used to provide power for the movement of the movable member 32 along the second direction Y.

[0052] The movable member 32 is provided with a movable portion 33 that can move along the third direction Z. A rack 34 extending longitudinally along the third direction Z is arranged on the movable portion 33. The rack 34 meshes with the external spline of the spline shaft 52. The sampling needle 31 is arranged on the movable portion 33. When the spline shaft 52 rotates around its own axis, through the cooperation of the external spline on the spline shaft 52 and the rack 34, the movable portion 33 can move along the third direction Z, and further the movable portion 33 can drive the sampling needle 31 to move in the third direction Z. And according to the different rotation directions of the spline shaft 52 around its own axis, the sampling needle 31 can be raised or lowered in the third direction Z.

[0053] In some embodiments, the second driving assembly 40 further includes driving wheels 42 and a timing belt 43. There are two driving wheels 42, and the two driving wheels 42 are arranged on the cantilever beam 20 at intervals along the second direction Y. The timing belt 43 is wound between the two driving wheels 42. The second driving member 41 is in transmission connection with the driving wheel 42 close to the first end 21 and is used to drive the driving wheel 42 to rotate, and drive the timing belt 43 to move through the rotation of the driving wheel 42.

[0054] Wherein, the timing belt 43 is connected to the movable member 32, and drives the movable member 32 to move in the second direction Y through the movement of the timing belt 43. At the same time, during the movement of the movable member 32 in the second direction Y, the rack 34 on the movable part 33 also moves along the external spline of the spline shaft 52, and the rack 34 and the external spline always remain in an engaged state during this process, so that when the movable member 32 moves to any position in the second direction Y, the rack 34 can be driven to move by rotating the spline shaft 52, thereby ensuring the normal operation of the sampling needle 31 in the third direction Z.

[0055] Specifically, in some embodiments, a second guide rail 44 extending longitudinally along the second direction Y is provided on the cantilever beam 20. The sampling assembly 30 includes multiple groups, and the movable member 32 of each group of the sampling assembly 30 is movably installed on the second guide rail 44. The multiple groups of the sampling assembly 30 include multiple sampling needles 31, and all the sampling needles 31 are installed on the cantilever beam 20 through the movable member 32, so that the cantilever beam 20 can drive multiple sampling needles 31 to move at one time, and perform sampling or sampling operations on multiple reagent containers through the multiple sampling needles 31 at the same time.

[0056] In order to adjust the gap between the multiple sampling needles 31 to adapt to reagent containers of different sizes, the second driving assembly 40 includes multiple groups. The movable member 32 of each sampling assembly 30 is connected to the timing belt 43 of one group of the second driving assembly 40, that is, each timing belt 43 drives each movable member 32 to move in the second direction Y, so as to adjust the position of each sampling needle 31 on the cantilever beam 20, so that the gap between two adjacent sampling needles 31 can meet the requirements of the reagent container.

[0057] Further, in order to enable each sampling needle 31 to move in the third direction Z, the third driving assembly 50 includes multiple groups. The rack 34 of each group of the sampling assembly 30 is engaged with the spline shaft 52 of one group of the third driving assembly 50, that is, each spline shaft 52 drives each rack 34 to move in the third direction Z, so as to adjust the position of each sampling needle 31 in the third direction Z, so that each sampling needle 31 can complete sampling or sampling actions individually or simultaneously.

[0058] Specifically, in one embodiment, the second driving assembly 40 includes two groups. Each group of the second driving assembly 40 includes a second driving member 41, a synchronous belt 43, and two driving wheels 42. The third driving assembly 50 also includes two groups. Each group of the third driving assembly 50 includes a third driving member 51, a spline shaft 52, and a coupling. The coupling is used to connect the third driving member 51 and the spline shaft 52. The sample adding assembly 30 also includes two groups. The sample adding needles 31 of each group of the sample adding assembly 30 are respectively moved in the second direction Y and the third direction Z through one group of the second driving assembly 40 and one group of the third driving assembly 50.

[0059] Among them, although each sample adding needle 31 can move independently in the second direction Y and the third direction Z, all the sample adding assemblies 30 are arranged on the cantilever beam 20. Therefore, all the sample adding needles 31 can only move simultaneously in the first direction X and cannot adjust the position of the sample adding needles 31 in the first direction X individually. However, in the actual use process, all the sample adding needles 31 may not be in a straight line in the second direction Y, resulting in the problem that one or more sample adding needles 31 cannot be aligned with the reagent container.

[0060] Therefore, in some embodiments, the sample adding assembly 30 further includes a mounting member 35 and an adjusting member 36. The sample adding needle 31 is mounted on the mounting member 35. The mounting member 35 is movably mounted on the adjusting member 36 along the first direction X. The adjusting member 36 is mounted on the movable part 33. When the relative position of the mounting member 35 and the adjusting member 36 in the first direction X changes, the relative position of the sample adding needle 31 and the movable part 33 in the first direction X also changes. Finally, the relative position of the sample adding needle 31 and the cantilever beam 20 in the first direction X is adjusted. In the actual use process, the relative position between the mounting member 35 and the adjusting member 36 on each sample adding needle 31 can be adjusted to adjust the position of each sample adding needle 31 relative to the cantilever beam 20, so that all the sample adding needles 31 can be located on the same straight line in the second direction Y, facilitating the operations of adding liquid and sucking liquid to multiple reagent containers simultaneously.

[0061] Specifically, in some embodiments, refer to Figure 3 , a chute 361 extending longitudinally along the first direction X is formed on the mounting member 35. A boss 362 is protrudingly provided on the adjusting member 36. The boss 362 is matched with the chute 361 to enable the mounting member 35 to move relative to the adjusting member 36 through the movement of the boss in the chute 361. It can be understood that in some other embodiments, the boss 362 may be provided on the mounting member 35 and the chute 361 may be provided on the adjusting member 36.

[0062] Among them, the sample adding assembly 30 includes an adjusting nut 363. The adjusting member 36 protrudes towards the mounting member 35 and is provided with an adjusting platform 364. A threaded hole extending longitudinally in the first direction X is formed in the adjusting platform 364. The adjusting nut 363 is threadedly connected to the threaded hole, and during the movement of the adjusting nut 363 within the threaded hole, the adjusting nut 363 can abut against the mounting member 35. In this way, when the user turns the adjusting nut 363, the adjusting nut 363 will gradually approach the mounting member 35 until it abuts against the mounting member 35. After that, if the user continues to turn the adjusting nut 363, the adjusting nut 363 will drive the mounting member 35 to move together along the first direction X, so as to realize fine adjustment of the mounting member 35 in the first direction X through the adjusting nut 363, and further fine adjust the position of the sampling needle 31 on the mounting member 35 in the first direction X.

[0063] In some embodiments, the sample adding assembly 30 further includes a retaining piece 37 and an elastic member 371. The elastic member 371 elastically connects the retaining piece 37 and the mounting member 35 and can elastically deform along the third direction Z. The sampling needle 31 is movably mounted on the mounting member 35 along the third direction Z and is connected to the retaining piece 37. When the mounting member 35 moves along the third direction Z under the action of the spline shaft 52 and the rack 34, the mounting member 35 drives the sampling needle 31 to move together through the retaining piece 37. If the sampling needle 31 encounters an obstacle or the bottom of the reagent container during the movement, the movement of the sampling needle 31 stops at this time, and the retaining piece 37 also stops moving together. However, at this time, the mounting member 35 will still continue to move. Due to the existence of the elastic member 371, the movement of the mounting member 35 will only compress the elastic member 371 and will not drive the sampling needle 31 and the retaining piece 37 to move, thus avoiding damage to the sampling needle 31.

[0064] Furthermore, the sample adding assembly 30 further includes an emergency stop sensor 372. The emergency stop sensor 372 is arranged on the mounting member 35 and is communicatively connected to the third driving member 51. A triggering portion 373 is arranged on the retaining piece 37. During the movement of the retaining piece 37 relative to the mounting member 35 along the third direction Z, the triggering portion 373 can trigger the emergency stop sensor 372, so that the emergency stop sensor 372 issues an emergency stop signal. After receiving the emergency stop signal, the third driving member 51 immediately stops operating, so that the mounting member 35 stops operating, avoiding damage to the sample adding assembly 30 caused by the continuous movement of the mounting member 35.

[0065] Specifically, the emergency stop sensor 372 is an anti-collision optocoupler. When the sampling needle 31 encounters an obstacle or the bottom of the reagent container, the sampling needle 31 and the retaining piece 37 stop moving. At this time, the mounting member 35 continues to move, causing relative movement between the retaining piece 37 and the mounting member 35. The triggering portion 373 can be inserted into the anti-collision optocoupler, thereby sending an emergency stop signal to stop the third driving member 51.

[0066] It should be noted that in some other embodiments, the emergency stop sensor 372 can also be a touch sensor, and the triggering part 373 can touch the touch sensor, so that the touch sensor emits an emergency stop signal.

[0067] In some embodiments of the present application, the sample adding assembly 30 further includes a liquid level detection member 38. The liquid level detection member 38 is arranged on the sample adding needle 31. After the sample adding needle 31 extends into the reagent container, the liquid level detection member 38 can detect whether the liquid level in the reagent container meets the use requirements, so as to alarm and feedback data.

[0068] Specifically, the sample adding assembly 30 further includes a liquid level detection board 381. The sample adding needle 31 includes an inner needle and an outer needle. Liquid level detection members 38 are arranged on both the inner needle and the outer needle. All the liquid level detection members 38 are electrically connected to the liquid level detection board 381. After the sample adding needle 31 extends into the reagent container, the inner needle and the outer needle contact the liquid surface, and the capacitance between the inner needle and the outer needle will change. The liquid level detection board 381 compares the detected value with the set value to judge the liquid level position and whether the liquid level in the container meets the use requirements.

[0069] The embodiments of the present application further provide a fully automatic immunofluorescence staining instrument, including the sample adding structure in any of the above embodiments. The sample adding needle 31 of the above sample adding structure can move in any direction through the first driving assembly 10, the second driving assembly 40 and the third driving assembly 50, so as to realize accurate, fast and reliable sample adding and sample sucking actions, and thus can realize the automation of immunohistochemical staining.

[0070] The technical features of the above embodiments can be combined arbitrarily. For the sake of brief description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0071] The above embodiments only represent several implementation manners of the present application, and the description is relatively specific and detailed, but it should not be understood as a limitation to the scope of the patent application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several deformations and improvements can be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A sample adding structure, characterized in that: The sample loading structure comprises: A first driving assembly (10) comprises a first guide rail (11) extending longitudinally along a first direction (X); a cantilever beam (20) extending longitudinally along a second direction (Y) and comprising a first end (21) and a second end (22) arranged opposite to each other, the first end (21) being movably arranged on the first guide rail (11) along the first direction (X), and the second end (22) extending out of the first guide rail (11); A sample loading assembly (30) comprising a sample loading needle (31), wherein the sample loading needle (31) is movably mounted on a portion of the cantilever beam (20) extending out of the first guide rail (11) in both a second direction (Y) and a third direction (Z); A second driving assembly (40) comprising a second driving member (41), wherein the second driving member (41) is disposed at the first end (21) and is used to provide power for the movement of the sample injection needle (31) along the second direction (Y); A third driving assembly (50), comprising a third driving member (51), wherein the third driving member (51) is disposed at the first end (21) and is used to provide power for the movement of the sample injection needle (31) along the third direction (Z); The first direction (X), the second direction (Y) and the third direction (Z) intersect each other and are not coplanar.

2. The sample adding structure according to claim 1, characterized in that: The third driving assembly (50) further comprises a spline shaft (52), wherein the spline shaft (52) is arranged on the cantilever beam (20) and extends longitudinally along the second direction (Y), and the third driving member (51) is drivingly connected to the spline shaft (52) and is used to drive the spline shaft (52) to rotate around its own axis; The sample loading assembly (30) further comprises a movable member (32), wherein the movable member (32) is movably mounted on the cantilever beam (20) along the second direction (Y), and the second driving member (41) is transmission-connected to the movable member (32) and is used to provide power for the movable member (32) to move along the second direction (Y); The movable member (32) is provided with a movable portion (33) movable along the third direction (Z), the movable portion (33) is provided with a rack (34) longitudinally extending along the third direction (Z), the rack (34) is meshed with the external spline of the spline shaft (52), and the sample injection needle (31) is arranged on the movable portion (33).

3. The sample adding structure according to claim 2, characterized in that: The second driving assembly (40) further comprises a driving wheel (42) and a synchronous belt (43), wherein the driving wheels (42) comprise two, the two driving wheels (42) being arranged on the cantilever beam (20) at intervals along the second direction (Y), the synchronous belt (43) being wound between the two driving wheels (42), the movable member (32) being connected to the synchronous belt (43), and the second driving member (41) being drivingly connected to the driving wheel (42) close to the first end (21) and being used for driving the driving wheel (42) to rotate.

4. The sample adding structure according to claim 3, characterized in that: The cantilever beam (20) is provided with a second guide rail (44) extending longitudinally along the second direction (Y); the sample loading assembly (30) comprises a plurality of groups, and the movable parts (32) of each group of the sample loading assembly (30) are movably mounted on the second guide rail (44); The second driving assembly (40) comprises a plurality of groups, and the movable parts (32) of each group of the sample adding assemblies (30) are connected to the synchronous belt (43) of one group of the second driving assemblies (40); And / or, the third driving assembly (50) includes a plurality of groups, and the rack (34) of each group of the sample loading assemblies (30) is meshed with the spline shaft (52) of one group of the third driving assemblies (50).

5. The sample adding structure according to claim 2, characterized in that: The sample loading assembly (30) further comprises a mounting member (35) and an adjusting member (36), the sample loading needle (31) being mounted on the mounting member (35), the mounting member (35) being movably mounted on the adjusting member (36) along the first direction (X), and the adjusting member (36) being mounted on the movable portion (33).

6. The sample adding structure according to claim 5, characterized in that: One of the mounting member (35) and the adjusting member (36) is provided with a slide groove (361) extending longitudinally along the first direction (X), and the other is provided with a boss (362) protruding therefrom, the boss (362) and the slide groove (361) being matched with each other; The sample loading assembly (30) further comprises an adjusting nut (363); the adjusting member (36) is provided with an adjusting platform (364) protruding toward the mounting member (35); the adjusting platform (364) is provided with a threaded hole extending longitudinally along the first direction (X); the adjusting nut (363) is threadedly connected to the threaded hole; and when the adjusting nut (363) moves in the threaded hole, the adjusting nut (363) can abut against the mounting member (35).

7. The sample adding structure according to claim 5, characterized in that: The sample loading component (30) further comprises a baffle (37) and an elastic member (371), wherein the elastic member (371) elastically connects the baffle (37) and the mounting member (35) and is capable of elastic deformation along the third direction (Z); The sample addition needle (31) is movably mounted on the mounting member (35) along the third direction (Z), and is connected to the baffle (37).

8. The sample adding structure according to claim 7, characterized in that: The sample adding component (30) further comprises an emergency stop sensor (372), wherein the emergency stop sensor (372) is arranged on the mounting member (35) and is communicatively connected to the third driving member (51); A trigger portion (373) is provided on the baffle (37), and when the baffle (37) moves along the third direction (Z) relative to the mounting member (35), the trigger portion (373) can trigger the emergency stop sensor (372).

9. The sample adding structure according to claim 1, characterized in that: The sample adding component (30) further comprises a liquid level detection component (38), wherein the liquid level detection component (38) is arranged on the sample adding needle (31).

10. A fully automatic immunofluorescence staining instrument, characterized in that: The method comprises the sample adding structure as described in any one of claims 1 to 9.

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