Sample adding liquid path system

Abstract

The utility model relates to the technical field of space transcriptomics, and provides a sample adding liquid path system, which comprises a first pump body, a second pump body, a third pump body and a fourth pump body, one end of the cleaning liquid suction pipeline is matched with the cleaning liquid source; the first valve body is a three-way valve, and one connector of the first valve body is connected with the end, away from the cleaning fluid source, of the cleaning fluid suction pipeline; one end of the liquid storage pipeline is connected with the other interface of the first valve body, and the other end is connected with the first pump body; one end of the cleaning liquid pumping pipeline is connected with the last connector of the first valve body; the cleaning fluid needle is arranged at one end, far away from the first valve body, of the cleaning fluid pumping pipeline; when the first valve body is closed, the cleaning liquid suction pipeline is communicated with the liquid storage pipeline; and when the first valve body is opened, the cleaning liquid pumping pipeline is communicated with the liquid storage pipeline. According to the sample adding liquid path system, tedious manual pipetting operation is not needed, time can be saved, errors caused by human factors can be reduced, and influences on experiments are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of spatial transcriptomics, and particularly relates to a sample addition liquid path system. Background Art

[0002] In current spatial transcript experiments (tissue permeabilization & gene expression), clips (single-hole & eight-hole) are usually used as reaction utensils. Through a pipette, the operations of adding liquid, mixing, and cleaning of reaction reagents are repeated manually. Since multiple reaction reagents and system liquids are involved, the manual pipetting operation is very cumbersome, which takes a lot of time and is prone to errors. It may increase the drying time of the sample and lead to test failure, resulting in a very large loss. Content of the Utility Model

[0003] Therefore, the technical problem to be solved by the utility model is that in the prior art, through a pipette, the operations of adding liquid, mixing, and cleaning of reaction reagents are repeated manually. Since multiple reaction reagents and system liquids are involved, the manual pipetting operation is very cumbersome, which takes a lot of time and is prone to errors. It may increase the drying time of the sample and lead to test failure, resulting in a very large loss. Thus, a sample addition liquid path system is provided.

[0004] To solve the above technical problem, the technical solution of the utility model is as follows:

[0005] The utility model provides a sample addition liquid path system, including: a first pump body for sucking or pumping a cleaning liquid; a cleaning liquid suction pipeline, one end of which is adapted to be arranged at a cleaning liquid source; a first valve body, the first valve body is a three-way valve, and one of the interfaces of the first valve body is connected to the end of the cleaning liquid suction pipeline far from the cleaning liquid source; a liquid storage pipeline, one end of which is connected to another interface of the first valve body, and the other end is connected to the first pump body; a cleaning liquid pumping pipeline, one end of which is connected to the last interface of the first valve body; a cleaning liquid needle, arranged at the end of the cleaning liquid pumping pipeline far from the first valve body; when the first valve body is closed, the cleaning liquid suction pipeline is communicated with the liquid storage pipeline; when the first valve body is opened, the cleaning liquid pumping pipeline is communicated with the liquid storage pipeline.

[0006] Further, the volume of the liquid storage pipeline is not less than the amount of cleaning liquid required for a single-round cleaning of an eight-hole clip.

[0007] Furthermore, the sample addition liquid circuit system also includes a second valve body and a third valve body; the second valve body is arranged on the pipe section of the cleaning liquid suction pipeline close to the cleaning liquid source; the third valve body is arranged on the pipe section of the cleaning liquid suction pipeline close to the first valve body, and the third valve body is a three-way valve, and the last interface of the third valve body is connected to the external air; when the second valve body and the third valve body are both in an open state, the cleaning liquid suction pipeline is connected to the cleaning liquid source, and when the second valve body and the third valve body are both in a closed state, the cleaning liquid suction pipeline is connected to the external atmosphere.

[0008] Furthermore, the sample addition liquid circuit system also includes: a pure water suction pipeline, one end of which is adapted to be set up in a pure water source; a second pump body, the inlet of the second pump body is connected to the end of the pure water suction pipeline away from the pure water source; a pure water pumping pipeline, one end of which is connected to the outlet of the second pump body; the first pump body is a plunger pump, the other end of the pure water pumping pipeline is connected to the inlet of the first pump body; the liquid storage pipeline is connected to the outlet of the first pump body.

[0009] Furthermore, the sample adding liquid circuit system also includes a fourth valve body, a fifth valve body and a connecting pipeline; the fourth valve body and the fifth valve body are both arranged on the pure water suction pipeline; one end of the connecting pipeline is connected to the pipe section of the pure water suction pipeline located between the fourth valve body and the fifth valve body, and the other end is connected to the pipe section of the cleaning liquid suction pipeline located between the second valve body and the third valve body; when the fourth valve body, the fifth valve body and the first valve body are in an open state, the pure water suction pipeline, the second pump body, the pure water pumping pipeline, the first pump body, the liquid storage pipeline and the cleaning liquid pumping pipeline are connected; when the fourth valve body and the third valve body are opened and the fifth valve body is closed, the pure water suction pipeline, the connecting pipeline and the cleaning liquid suction pipeline are connected.

[0010] Furthermore, the sample addition liquid circuit system also includes a cleaning station, an outer wall cleaning pipeline and a sixth valve body; the cleaning station has an outer wall cleaning tank for accommodating the cleaning liquid needle; the sixth valve body is a three-way valve, and the sixth valve body is arranged on the pure water pumping pipeline; one end of the outer wall cleaning pipeline is connected to the last interface of the sixth valve body, and the other end extends to the outer wall cleaning tank; when the sixth valve body is opened, the pure water pumping pipeline is connected to the first pump body; when the sixth valve body is closed, the pure water pumping pipeline is connected to the outer wall cleaning pipeline.

[0011] Furthermore, the sample addition liquid path system also includes a first waste pipe, a second waste pipe and a third pump body; one end of the first waste pipe is connected to the outer wall cleaning tank, and the other end is connected to the inlet of the third pump body; one end of the second waste pipe is connected to the outlet of the third pump body.

[0012] Further, the sample addition liquid path system further includes a waste discharge needle, a third waste discharge pipeline, a fourth waste discharge pipeline, and a fourth pump body; the waste discharge needle is arranged in parallel with the cleaning liquid needle, and the needle tip end of the waste discharge needle extends beyond the needle tip end of the cleaning liquid needle; one end of the third waste discharge pipeline is connected to the waste discharge needle, and the other end is connected to the inlet of the fourth pump body; one end of the fourth waste discharge pipeline is connected to the outlet of the fourth pump body.

[0013] Further, the sample addition liquid path system further includes an inner wall cleaning pipeline and a seventh valve body; the seventh valve body is a three-way valve, and the seventh valve body is arranged on the outer wall cleaning pipeline; the cleaning station has an inner wall cleaning groove for accommodating the cleaning liquid needle; one end of the inner wall cleaning pipeline is connected to the last interface of the seventh valve body, and the other end extends to the inner wall cleaning groove; when the sixth valve body is closed and the seventh valve body is opened, the outer wall cleaning pipeline is communicated with the pure water pumping pipeline; when the sixth valve body and the seventh valve body are closed, the inner wall cleaning pipeline is communicated with the pure water pumping pipeline.

[0014] Further, the sample addition liquid path system further includes a temporary storage liquid suction pipeline, a temporary storage liquid pumping pipeline, and a fifth pump body; the cleaning station has a cleaning liquid temporary storage tank; one end of the temporary storage liquid suction pipeline is adapted to be arranged in connection with a cleaning liquid source, and the other end is connected to the inlet of the fifth pump body; one end of the temporary storage liquid pumping pipeline is connected to the outlet of the fifth pump body, and the other end extends to the cleaning liquid temporary storage tank.

[0015] Further, the sample addition liquid path system further includes a waste liquid tank, a cleaning liquid tank, and a pure water tank; the waste liquid tank is used for collecting waste liquid; the cleaning liquid tank is used for accommodating cleaning liquid; the pure water tank is used for accommodating pure water.

[0016] Further, the sample addition liquid path system further includes a sixth pump body and a pipette; one end of the pipette is connected to the sixth pump body, and the other end is used for connecting with different pipette tips.

[0017] The technical solution of the present utility model has the following advantages:

[0018] When the sample addition liquid path system provided by the present utility model is in use, by switching the working state of the first valve body, the first pump body is used to automatically suck or pump out the cleaning liquid, eliminating the need for manual and cumbersome pipetting operations. This not only saves time but also reduces errors caused by human factors and avoids affecting the experiment. Description of the Drawings

[0019] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 It is a schematic diagram of the sample addition liquid path system in the embodiment of the present invention;

[0021] Figure 2 It is a schematic diagram of the pipette and the sixth pump body in the sample addition liquid path system in the embodiment of the present invention;

[0022] Figure 3 It is a schematic diagram of the cleaning station in the sample addition liquid path system in the embodiment of the present invention.

[0023] Explanation of the reference numerals:

[0024] 1. First pump body; 2. Cleaning liquid suction pipeline; 3. First valve body; 4. Liquid storage pipeline; 5. Cleaning liquid pumping pipeline; 6. Cleaning liquid needle; 7. Second valve body; 8. Third valve body; 9. Pure water suction pipeline; 10. Second pump body; 11. Pure water pumping pipeline; 12. Fourth valve body; 13. Fifth valve body; 14. Connection pipeline; 15. Cleaning station; 16. Outer wall cleaning pipeline; 17. Sixth valve body; 18. Outer wall cleaning tank; 19. First waste discharge pipeline; 20. Second waste discharge pipeline; 21. Third pump body; 22. Waste discharge needle; 23. Third waste discharge pipeline; 24. Fourth waste discharge pipeline; 25. Fourth pump body; 26. Inner wall cleaning pipeline; 27. Seventh valve body; 28. Inner wall cleaning tank; 29. Temporary storage liquid suction pipeline; 30. Temporary storage liquid pumping pipeline; 31. Fifth pump body; 32. Waste liquid tank; 33. Cleaning liquid tank; 34. Pure water tank; 35. Sixth pump body; 36. Pipette; 37. Pipette tip; 38. Cleaning liquid temporary storage tank; 39. Eighth valve body; 40. Overflow tank. Specific embodiments

[0025] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the drawings. Obviously, the described embodiments are some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0026] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0027] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0028] In addition, the technical features involved in different embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0029] As Figure 1 shown, this embodiment provides a sample addition liquid path system, including: a first pump body 1 for sucking or pumping a cleaning liquid; a cleaning liquid suction pipeline 2 with one end adapted to be arranged at a cleaning liquid source; a first valve body 3, the first valve body 3 being a three-way valve, and one of the interfaces of the first valve body 3 being connected to the end of the cleaning liquid suction pipeline 2 away from the cleaning liquid source; a liquid storage pipeline 4 with one end connected to another interface of the first valve body 3 and the other end connected to the first pump body 1; a cleaning liquid pumping pipeline 5 with one end connected to the last interface of the first valve body 3; a cleaning liquid needle 6 arranged at the end of the cleaning liquid pumping pipeline 5 away from the first valve body 3; when the first valve body 3 is closed, the cleaning liquid suction pipeline 2 is communicated with the liquid storage pipeline 4; when the first valve body 3 is opened, the cleaning liquid pumping pipeline 5 is communicated with the liquid storage pipeline 4. For example, the cleaning liquid can be an SSC reagent.

[0030] For the sample addition liquid path system provided in this embodiment, during use, by switching the working state of the first valve body 3, the first pump body 1 is used to automatically suck or pump out the cleaning liquid, eliminating the need for manual and cumbersome pipetting operations. This not only saves time but also reduces errors caused by human factors and avoids affecting the experiment.

[0031] Among them, the volume of the liquid storage pipeline 4 is not less than the amount of cleaning liquid required for a single-round cleaning of the eight-hole clip. For example, the liquid storage pipeline 4 can be a pipeline with a diameter of 0.8 cm and a length of 3.5 m.

[0032] Among them, the sample addition liquid path system further includes a second valve body 7 and a third valve body 8; the second valve body 7 is arranged on the pipe section of the cleaning liquid suction pipeline 2 close to the cleaning liquid source; the third valve body 8 is arranged on the pipe section of the cleaning liquid suction pipeline 2 close to the first valve body 3, and the third valve body 8 is a three-way valve, and the last interface of the third valve body 8 communicates with the external air; when both the second valve body 7 and the third valve body 8 are in the open state, the cleaning liquid suction pipeline 2 communicates with the cleaning liquid source, and when both the second valve body 7 and the third valve body 8 are in the closed state, the cleaning liquid suction pipeline 2 communicates with the external atmosphere.

[0033] Among them, the sample addition liquid path system further includes: a pure water suction pipeline 9, one end of which is adapted to be arranged at the pure water source; a second pump body 10, the inlet of the second pump body 10 is connected to the end of the pure water suction pipeline 9 far from the pure water source; a pure water pumping pipeline 11, one end of which is connected to the outlet of the second pump body 10; the first pump body 1 is a plunger pump, and the other end of the pure water pumping pipeline 11 is connected to the inlet of the first pump body 1; the liquid storage pipeline 4 is connected to the outlet of the first pump body 1. For example, the second pump body 10 can be a diaphragm pump.

[0034] Among them, the sample addition liquid path system further includes a fourth valve body 12, a fifth valve body 13 and a connecting pipeline 14; both the fourth valve body 12 and the fifth valve body 13 are arranged on the pure water suction pipeline 9; one end of the connecting pipeline 14 is connected to the pipe section of the pure water suction pipeline 9 between the fourth valve body 12 and the fifth valve body 13, and the other end is connected to the pipe section of the cleaning liquid suction pipeline 2 between the second valve body 7 and the third valve body 8; when the fourth valve body 12, the fifth valve body 13 and the first valve body 3 are in the open state, the pure water suction pipeline 9, the second pump body 10, the pure water pumping pipeline 11, the first pump body 1, the liquid storage pipeline 4 and the cleaning liquid pumping pipeline 5 are conducted; when the fourth valve body 12 and the third valve body 8 are open and the fifth valve body 13 is closed, the pure water suction pipeline 9, the connecting pipeline 14 and the cleaning liquid suction pipeline 2 are conducted.

[0035] Among them, the sample addition liquid path system further includes a cleaning station 15, an outer wall cleaning pipeline 16 and a sixth valve body 17; the cleaning station 15 has an outer wall cleaning tank 18 for accommodating the cleaning liquid needle 6; the sixth valve body 17 is a three-way valve, and the sixth valve body 17 is arranged on the pure water pumping pipeline 11; one end of the outer wall cleaning pipeline 16 is connected to the last interface of the sixth valve body 17, and the other end extends to the outer wall cleaning tank 18; when the sixth valve body 17 is open, the pure water pumping pipeline 11 is conducted with the first pump body 1; when the sixth valve body 17 is closed, the pure water pumping pipeline 11 is conducted with the outer wall cleaning pipeline 16.

[0036] Wherein, the sample addition liquid path system further includes a first waste discharge pipeline 19, a second waste discharge pipeline 20 and a third pump body 21; one end of the first waste discharge pipeline 19 is communicated with the outer wall cleaning tank 18, and the other end is connected to the inlet of the third pump body 21; one end of the second waste discharge pipeline 20 is connected to the outlet of the third pump body 21. For example, the third pump body 21 can be a diaphragm pump.

[0037] Wherein, the sample addition liquid path system further includes a waste discharge needle 22, a third waste discharge pipeline 23, a fourth waste discharge pipeline 24 and a fourth pump body 25; the waste discharge needle 22 is arranged in parallel with the cleaning liquid needle 6, and the needle tip end of the waste discharge needle 22 extends beyond the needle tip end of the cleaning liquid needle 6; one end of the third waste discharge pipeline 23 is connected to the waste discharge needle 22, and the other end is connected to the inlet of the fourth pump body 25; one end of the fourth waste discharge pipeline 24 is connected to the outlet of the fourth pump body 25. For example, the fourth pump body 25 can be a peristaltic pump.

[0038] Wherein, the sample addition liquid path system further includes an inner wall cleaning pipeline 26 and a seventh valve body 27; the seventh valve body 27 is a three-way valve, and the seventh valve body 27 is arranged on the outer wall cleaning pipeline 16; the cleaning station 15 has an inner wall cleaning tank 28 for accommodating the cleaning liquid needle 6; one end of the inner wall cleaning pipeline 26 is connected to the last interface of the seventh valve body 27, and the other end extends to the inner wall cleaning tank 28; when the sixth valve body 17 is closed and the seventh valve body 27 is opened, the outer wall cleaning pipeline 16 is communicated with the pure water pumping pipeline 11; when the sixth valve body 17 and the seventh valve body 27 are closed, the inner wall cleaning pipeline 26 is communicated with the pure water pumping pipeline 11.

[0039] Wherein, the sample addition liquid path system further includes a temporary storage liquid suction pipeline 29, a temporary storage liquid pumping pipeline 30 and a fifth pump body 31; the cleaning station 15 has a cleaning liquid temporary storage tank 38; one end of the temporary storage liquid suction pipeline 29 is adapted to be arranged according to the cleaning liquid source, and the other end is connected to the inlet of the fifth pump body 31; one end of the temporary storage liquid pumping pipeline 30 is connected to the outlet of the fifth pump body 31, and the other end extends to the cleaning liquid temporary storage tank 38. For example, the fifth pump body 31 can be a peristaltic pump. For example, when the temporary storage liquid suction pipeline 29 is communicated with the cleaning liquid suction pipeline 2, an eighth valve body 39 can be arranged on the pipe section of the temporary storage liquid suction pipeline 29 close to the cleaning liquid suction pipeline 2 to control the on-off of the temporary storage liquid suction pipeline 29.

[0040] Wherein, the sample addition liquid path system further includes a waste liquid tank 32, a cleaning liquid tank 33 and a pure water tank 34; the waste liquid tank 32 is used for collecting waste liquid; the cleaning liquid tank 33 is used for accommodating cleaning liquid; the pure water tank 34 is used for accommodating pure water.

[0041] Such as Figure 2As shown in the figure, the sample addition liquid path system further includes a sixth pump body 35 and a pipette 36; one end of the pipette 36 is connected to the sixth pump body 35, and the other end is used to be connected to different pipette tips 37. For example, the sixth pump body 35 can be a plunger pump. With such a setting, the sixth pump body 35 is connected to the pipette tip and is used for various liquid addition and drainage in gene expression experiments. Each time a new pipette tip 37 is replaced, the whole process ensures no pollution; only partial reagent addition is achieved in tissue permeabilization experiments.

[0042] As Figure 3 shown in the figure, for the cleaning station 15, an overflow tank 40 can be provided between the inner wall cleaning tank 28 and the cleaning liquid storage tank 38. When the liquid level in the inner wall cleaning tank 28 or the cleaning liquid storage tank 38 reaches a certain height, the liquid overflows outward and enters the overflow tank 40. The bottom of the overflow tank 40 has an opening, and the bottom of the outer wall cleaning tank 18 also has an opening. For example, the first waste drainage pipeline 19 can be divided into two branches, one branch communicates with the opening at the bottom of the overflow tank 40, and the other branch communicates with the opening at the bottom of the outer wall cleaning tank 18.

[0043] Among them, the sample addition liquid path system in this application can also be provided with a controller, which is electrically connected to each pump body and valve body and is used to control the working states of the pump body and valve body.

[0044] During use, the sample addition liquid path system mainly has the following basic operations: SSC filling before testing, SSC filling during testing, needle drainage, needle liquid addition, cleaning of the waste drainage needle 22 (inner wall cleaning, outer wall cleaning), SSC hole filling, pipette tip liquid extraction and liquid addition. The specific control process is as follows:

[0045] SSC filling before testing: The cleaning liquid needle 6 needs to be moved to the waste liquid tank 32 position. Open the first valve body 3, the fourth valve body 12, the fifth valve body 13, the sixth valve body 17, as well as the second pump body 10 and the third pump body 21, so that pure water passes through the pure water suction pipeline 9, the pure water pumping pipeline 11, the liquid storage pipeline 4, and the cleaning liquid pumping pipeline 5 to fill the first pump body 1 and the liquid storage pipeline 4 (until pure water is seen coming out of the cleaning liquid needle 6, then close the second pump body 10, the first valve body 3, the fourth valve body 12, the fifth valve body 13, and the sixth valve body 17). For example, the pipeline can be filled in 15 s. This filling is to fill the glass tube of the first pump body 1 with pure water to avoid being unable to suck the cleaning liquid subsequently. Then, open the second valve body 7 and the third valve body 8, set the number of steps of the first pump body 1 to -10000 and the speed to 15, and fill the SSC into the cleaning liquid suction pipeline 2 for pipeline rinsing. Close the second valve body 7 and the third valve body 8, then open the third valve body 8 and the fourth valve body 12, set the number of steps of the first pump body 1 to -9400 and the speed to 15, and fill the pure water suction pipeline 9, the connection pipeline 14, and the cleaning liquid suction pipeline 2 with pure water until the pure water just passes through the first valve body 3 for pipeline rinsing. The first pump body 1 performs a reset action, and close the third valve body 8 and the fourth valve body 12 (keep the first valve body 3 open when closing the third valve body 8). Then open the first valve body 3, the fourth valve body 12, the fifth valve body 13, the sixth valve body 17, as well as the second pump body 10 and the third pump body 21, so that pure water passes through the pure water suction pipeline 9, the pure water pumping pipeline 11, the liquid storage pipeline 4, and the cleaning liquid pumping pipeline 5 to fill the first pump body 1 and the liquid storage pipeline 4 (until pure water is seen coming out of the cleaning liquid needle 6, then close the second pump body 10, the first valve body 3, the fourth valve body 12, the fifth valve body 13, and the sixth valve body 17). The pipeline can be filled in 15 s. This filling is to fill the liquid storage pipeline 4 with pure water and flush the residual cleaning liquid in each pipeline. Set the number of steps of the first pump body 1 to -2000 and the speed to 15, keep the third valve body 8 closed, and pre-absorb air. Open the second valve body 7 and the third valve body 8, set the number of steps of the first pump body 1 to -21000 and the speed to 15, so that the SSC passes through the cleaning liquid suction pipe and the liquid storage pipeline 4. Close the second valve body 7 and the third valve body 8. Set the number of steps of the first pump body 1 to -2000 and the speed to 15, and suck air through the air port of the third valve body 8 (keep the third valve body 8 closed) to make the end of the SSC reagent pass through the first valve body 3. Open the first valve body 3, the first pump body 1 pushes in the reverse direction, set the number of steps to 9500 and the speed to 15, and pump out the pure water in the cleaning liquid pumping pipeline 5 and fill the SSC. Open the fourth pump body 25, set the parameter speed to 20 and the number of steps to 3000, and suck the SSC at the tip of the waste discharge needle 22 back into the waste discharge needle 22 to avoid contamination.

[0046] Filling SSC during the test process: The cleaning liquid needle 6 needs to be moved to the position of the waste liquid tank 32. The first pump body 1 performs a reset action. Then, the second valve body 7 and the third valve body 8 are opened. The first pump body 1 is set with a step number of -21000 and a speed of 15, so that SSC passes through the cleaning liquid suction pipe and the liquid storage pipeline 4. Then, the second valve body 7 and the third valve body 8 are closed. The first pump body 1 is set with a step number of -2000 and a speed of 15 (the third valve body 8 is closed), sucks air through the air port of the third valve body 8, the first pump body 1 pushes in the reverse direction. The first pump body 1 is set with a step number of 9500 and a speed of 15 and the first valve body 3 is opened to pump the SSC in the cleaning liquid pumping pipeline 5 to fill it, and then the first valve body 3 is closed. The fourth pump body 25 is opened, and the parameters are set with a speed of 20 and a step number of 3000 to suck the SSC at the tip of the waste discharge needle 22 back into the waste discharge needle 22.

[0047] Needle liquid discharge: The waste discharge needle 22 is moved to the collet hole position and the fourth pump body 25 is opened to perform the waste liquid discharge action. After discharging the waste liquid, the fourth pump body 25 is closed.

[0048] Needle liquid addition: The cleaning liquid needle 6 is moved to the collet hole position to perform the liquid addition action. The first pump body 1 is set with a step number of 1900 and a speed of 15, and the first valve body 3 is opened for liquid addition. The SSC in the liquid storage pipeline 4 and the cleaning liquid pumping pipeline 5 is pumped into the collet hole by the reverse push of the first pump body 1, and then the first valve body 3 is closed.

[0049] Cleaning of the waste discharge needle 22: When cleaning the outer wall of the waste discharge needle 22, the waste discharge needle 22 is moved to the outer wall cleaning tank 18, the third pump body 21 is opened, and then the fourth valve body 12, the fifth valve body 13 and the second pump body 10 are opened, so that pure water passes through the pure water suction pipeline 9 and the outer wall cleaning pipeline 16 until the pure water is added to the outer wall cleaning tank 18 to clean the outer wall of the waste discharge needle 22. The waste liquid is sucked into the waste liquid tank 32 through the first waste discharge pipeline 19 and the second waste discharge pipeline 20. After the cleaning is completed, the second pump body 10 and the fourth valve body 12 and the fifth valve body 13 are closed.

[0050] When cleaning the inner wall of the waste discharge needle 22, the waste discharge needle 22 is moved to the inner wall cleaning port, the third pump body 21 is opened, and then the fourth valve body 12, the fifth valve body 13, the seventh valve body 27 and the second pump body 10 are opened so that pure water passes through the pure water suction pipeline 9 and the inner wall cleaning pipeline 26 until the pure water is added to the inner wall cleaning tank 28, and the fourth pump body 25 is opened to suck the pure water, so that the pure water enters the waste liquid tank 32 through the third waste discharge pipeline 23 and the fourth waste discharge pipeline 24, and the overflowing pure water is sucked into the waste liquid tank 32 through the first waste discharge pipeline 19 and the second waste discharge pipeline 20. After the cleaning is completed, the second pump body 10, the third pump body 21 and the fourth valve body 12, the fifth valve body 13, the seventh valve body 27 are closed and the waste discharge needle 22 is lifted for pipeline emptying. After the pipeline is emptied, the fourth pump body 25 is closed.

[0051] SSC Hole Filling: Open the second valve body 7 and the fifth pump body 31 (speed 50, number of steps 46000), so that SSC enters the cleaning liquid storage tank 38 after passing through the temporary liquid suction pipeline 29 and the temporary liquid pumping pipeline 30. Open the third pump body 21 to suck the overflowing SSC into the waste liquid tank 32 through the first waste discharge pipeline 19 and the second waste discharge pipeline 20, and fill the cleaning liquid storage tank 38 (about 155 ul).

[0052] Pipette Tip Liquid Withdrawal and Liquid Addition: Move the pipette tip 37 to the reagent position to withdraw the reagent (the reagent volume is adjustable). After the pipette tip 37 withdraws the reagent, move it to the cassette hole position for liquid addition. The liquid addition volume is adjustable, and the instruction of sucking more and adding less can be performed.

[0053] In summary, in the sample addition liquid path system of the present application, the third valve body 8 can introduce air to isolate different reagents, and problems such as diffusion, dilution, and contamination will not occur.

[0054] In the sample addition liquid path system of the present application, the storage pipeline 4 can store enough SSC to ensure that the SSC in the pipeline meets the cleaning requirements for eight holes in a single round; at the same time, it can prevent SSC from entering the first pump body 1 and avoid diffusion contamination.

[0055] In the sample addition liquid path system of the present application, the plunger pump has a slow speed and is used for precise metering; the peristaltic pump has a relatively fast speed and is used for rough metering; the diaphragm pump is not used for metering and is used for strong cleaning or filling.

[0056] Obviously, the above embodiments are only examples for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A sample addition liquid path system, characterized in that, Comprising: A first pump body (1) for sucking or pumping a cleaning liquid; A cleaning liquid suction pipeline (2) with one end adapted to be arranged at a cleaning liquid source; A first valve body (3), the first valve body (3) being a three-way valve, and one of the interfaces of the first valve body (3) being connected to the end of the cleaning liquid suction pipeline (2) away from the cleaning liquid source; A liquid storage pipeline (4) with one end connected to another interface of the first valve body (3) and the other end connected to the first pump body (1); A cleaning liquid pumping pipeline (5) with one end connected to the last interface of the first valve body (3); A cleaning liquid needle (6) arranged at the end of the cleaning liquid pumping pipeline (5) away from the first valve body (3); When the first valve body (3) is closed, the cleaning liquid suction pipeline (2) is communicated with the liquid storage pipeline (4); when the first valve body (3) is opened, the cleaning liquid pumping pipeline (5) is communicated with the liquid storage pipeline (4).

2. The sample addition liquid path system according to claim 1, wherein The volume of the liquid storage pipeline (4) is not less than the amount of cleaning liquid required for a single-round cleaning of an eight-hole clip.

3. The sample addition liquid path system according to claim 1, wherein It further includes a second valve body (7) and a third valve body (8); The second valve body (7) is arranged on the pipeline section of the cleaning liquid suction pipeline (2) close to the cleaning liquid source; The third valve body (8) is arranged on the pipeline section of the cleaning liquid suction pipeline (2) close to the first valve body (3), and the third valve body (8) is a three-way valve, and the last interface of the third valve body (8) is communicated with the outside air; When both the second valve body (7) and the third valve body (8) are in the open state, the cleaning liquid suction pipeline (2) is communicated with the cleaning liquid source; when both the second valve body (7) and the third valve body (8) are in the closed state, the cleaning liquid suction pipeline (2) is communicated with the outside atmosphere.

4. The sample addition liquid path system according to claim 3, wherein It further includes: A pure water suction pipeline (9) with one end adapted to be arranged at a pure water source; A second pump body (10) with the inlet of the second pump body (10) connected to the end of the pure water suction pipeline (9) away from the pure water source; A pure water pumping pipeline (11) with one end connected to the outlet of the second pump body (10); The first pump body (1) is a plunger pump, and the other end of the pure water pumping pipeline (11) is connected to the inlet of the first pump body (1); the liquid storage pipeline (4) is connected to the outlet of the first pump body (1).

5. The sample addition liquid path system according to claim 4, wherein It further includes a fourth valve body (12), a fifth valve body (13) and a connecting pipeline (14); Both the fourth valve body (12) and the fifth valve body (13) are arranged on the pure water suction pipeline (9); One end of the connecting pipeline (14) is connected to the pipeline section of the pure water suction pipeline (9) between the fourth valve body (12) and the fifth valve body (13), and the other end is connected to the pipeline section of the cleaning liquid suction pipeline (2) between the second valve body (7) and the third valve body (8); When the fourth valve body (12), the fifth valve body (13) and the first valve body (3) are in an open state, the pure water suction pipeline (9), the second pump body (10), the pure water pumping pipeline (11), the first pump body (1), the liquid storage pipeline (4) and the cleaning liquid pumping pipeline (5) are connected; When the fourth valve body (12) and the third valve body (8) are opened and the fifth valve body (13) is closed, the pure water suction pipeline (9), the connecting pipeline (14) and the cleaning liquid suction pipeline (2) are connected.

6. The sample addition liquid path system according to claim 5, characterized in that: It also includes a cleaning station (15), an outer wall cleaning pipeline (16) and a sixth valve body (17); The cleaning station (15) has an outer wall cleaning groove (18) for accommodating the cleaning liquid needle (6); The sixth valve body (17) is a three-way valve, and the sixth valve body (17) is arranged on the pure water pumping pipeline (11); One end of the outer wall cleaning pipeline (16) is connected to the last interface of the sixth valve body (17), and the other end extends to the outer wall cleaning tank (18); When the sixth valve body (17) is opened, the pure water pumping pipeline (11) is connected to the first pump body (1); when the sixth valve body (17) is closed, the pure water pumping pipeline (11) is connected to the outer wall cleaning pipeline (16).

7. The sample addition liquid path system according to claim 6, characterized in that: It also includes a first waste pipe (19), a second waste pipe (20) and a third pump body (21); One end of the first waste discharge pipeline (19) is connected to the outer wall cleaning tank (18), and the other end is connected to the inlet of the third pump body (21); One end of the second waste pipe (20) is connected to the outlet of the third pump body (21).

8. The sample addition liquid path system according to claim 6, characterized in that: It also includes a waste discharge needle (22), a third waste discharge pipeline (23), a fourth waste discharge pipeline (24) and a fourth pump body (25); The waste discharge needle (22) and the cleaning liquid needle (6) are arranged in parallel, and the needle opening end of the waste discharge needle (22) exceeds the needle opening end of the cleaning liquid needle (6); One end of the third waste discharge pipeline (23) is connected to the waste discharge needle (22), and the other end is connected to the inlet of the fourth pump body (25); One end of the fourth waste discharge pipeline (24) is connected to the outlet of the fourth pump body (25).

9. The sample addition liquid path system according to claim 8, characterized in that: It also includes an inner wall cleaning pipeline (26) and a seventh valve body (27); The seventh valve body (27) is a three-way valve, and the seventh valve body (27) is arranged on the outer wall cleaning pipeline (16); The cleaning station (15) has an inner wall cleaning groove (28) for accommodating a cleaning liquid needle (6); One end of the inner wall cleaning pipeline (26) is connected to the last interface of the seventh valve body (27), and the other end extends to the inner wall cleaning groove (28); When the sixth valve body (17) is closed and the seventh valve body (27) is open, the outer wall cleaning pipeline (16) is communicated with the pure water pumping pipeline (11); when the sixth valve body (17) and the seventh valve body (27) are closed, the inner wall cleaning pipeline (26) is communicated with the pure water pumping pipeline (11).

10. The sample addition liquid path system according to claim 6, wherein it further includes a temporary storage liquid suction pipeline (29), a temporary storage liquid pumping pipeline (30) and a fifth pump body (31); the cleaning station (15) has a cleaning liquid temporary storage tank (38); one end of the temporary storage liquid suction pipeline (29) is adapted to be arranged at a cleaning liquid source, and the other end is connected to the inlet of the fifth pump body (31); one end of the temporary storage liquid pumping pipeline (30) is connected to the outlet of the fifth pump body (31), and the other end extends to the cleaning liquid temporary storage tank (38).

11. The sample addition liquid path system according to claim 6, wherein it further includes a waste liquid tank (32), a cleaning liquid tank (33) and a pure water tank (34); the waste liquid tank (32) is used for collecting waste liquid; the cleaning liquid tank (33) is used for containing cleaning liquid; the pure water tank (34) is used for containing pure water.

12. The sample addition liquid path system according to claim 1, wherein it further includes a sixth pump body (35) and a pipette (36); one end of the pipette (36) is connected to the sixth pump body (35), and the other end is used for connecting to different pipette tips (37).

Images