Micro flow cytometer

Abstract

The utility model is suitable for the technical field of flow cytometers, and provides a micro flow cytometer, which comprises a micro flow cytometer body, the detection cavity is formed in the outer side wall of the micro flow cytometer body; the rotating cylinder is fixed at the central position of the bottom in the detection cavity through a bolt; the turntable is fixed at the output end of the rotating cylinder; the test tube clamping groove is formed in the outer side wall of the turntable; the first supporting plate and the second supporting plate are fixed to the positions, close to the outer sides of the test tube clamping grooves, of the top of the rotating disc through bolts, and the first supporting plate is located on one side of the second supporting plate; the micro electric push rods are fixed on the outer side walls of the first supporting plate and the second supporting plate through bolts; the test tubes are clamped and supported by the test tube clamping and supporting plate, so that the stability of the test tubes during collection can be ensured, and the test tubes are supported and lifted by the test tube sleeves, so that the micro flow cytometer body can realize loading and unloading of the test tubes in a working state, and automatic replacement of the test tubes is realized.

Description

Technical Field

[0001] The utility model belongs to the technical field of flow cytometers, and particularly relates to a micro flow cytometer. Background Art

[0002] A flow cytometer is a device for automatically analyzing and sorting cells. It can quickly measure, store, and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in a liquid, and can sort out a specified cell subset according to a preselected parameter range. The volume of a flow cytometer has micro, small, medium, and large types.

[0003] After the existing micro flow cytometer completes the detection, the tester needs to replace the test tube. When replacing the test tube, the instrument needs to be in a standby state, which results in low working efficiency of the micro flow cytometer.

[0004] Therefore, a micro flow cytometer is proposed. Summary of the Utility Model

[0005] The utility model provides a micro flow cytometer, aiming to solve the above problems.

[0006] The utility model is realized as follows: A micro flow cytometer includes: a micro flow cytometer body; a detection chamber opened on the outer side wall of the micro flow cytometer body; a rotary cylinder fixed to the central position at the bottom inside the detection chamber by bolts; a turntable fixed to the output end of the rotary cylinder; test tube slots opened on the outer side wall of the turntable; a first support plate and a second support plate fixed to the top of the turntable near the outer side of the test tube slots by bolts, with the first support plate located on one side of the second support plate; micro electric push rods fixed to the outer side walls of the first support plate and the second support plate by bolts; a test tube clamping support plate fixed to the output end of the micro electric push rod; a telescopic cylinder fixed to the bottom inside the detection chamber near one side of the rotary cylinder by bolts; a lifting platform fixed to the output end of the telescopic cylinder; a motor fixed to the central position on the outer side wall of the lifting platform by bolts; a ball screw fixed to the output end of the motor; a moving plate fixed to the nut seat on the ball screw by screws; and a test tube sleeve arranged on the top of the moving plate.

[0007] Preferably, four test tube slots are provided, and the four test tube slots are symmetrically opened on the outer side wall of the turntable. A groove with a semi-circular cross-section is opened on the inner side wall of the test tube slot.

[0008] Preferably, the cross-section of the test tube clamping support plate is a semi-circular ring structure.

[0009] Preferably, four telescopic cylinders are provided, and every two of the four telescopic cylinders form a group. The two groups of telescopic cylinders are arranged at a 90-degree angle at the inner bottom of the detection chamber.

[0010] Preferably, a through groove for the ball screw to rotate is formed at the top of the lifting table.

[0011] Preferably, the output end of the rotary cylinder rotates 90 degrees each time.

[0012] Compared with the prior art, the embodiments of the present application mainly have the following beneficial effects:

[0013] By clamping and lifting the test tube with the test tube clamping tray, the stability of the test tube during collection can be ensured, and the test tube can be lifted up and down by using the test tube sleeve, so that the micro flow cytometer body can realize the loading and unloading of the test tube in the working state, and the automatic replacement of the test tube can be realized. This not only ensures the working efficiency of the micro flow cytometer, but also eliminates the need for manual replacement, saving manpower and avoiding the problem of pollution caused by the spillage of the collected liquid in the test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic structural diagram of the present utility model;

[0015] Figure 2 is a schematic structural diagram of the turntable of the present utility model;

[0016] Figure 3 is a schematic structural diagram of the lifting table of the present utility model;

[0017] Figure 4 is a schematic structural diagram of the test tube clamping tray of the present utility model.

[0018] In the figure: 1, micro flow cytometer body; 2, rotary cylinder; 3, turntable; 4, test tube slot; 5, first support plate; 6, second support plate; 7, micro electric push rod; 8, test tube clamping tray; 9, telescopic cylinder; 10, lifting table; 11, motor; 12, ball screw; 13, moving plate; 14, test tube sleeve; 15, detection chamber. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs; the terms used in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above drawings are intended to cover non-exclusive inclusion. The terms "first", "second", etc. in the specification and claims of this application or the above drawings are used to distinguish different objects and not to describe a specific order.

[0020] References to "embodiments" in this specification mean that the particular features, structures, or characteristics described in connection with the embodiments can be included in at least one embodiment of the present application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those skilled in the art will explicitly and implicitly understand that the embodiments described herein can be combined with other embodiments.

[0021] An embodiment of the present utility model provides a micro flow cytometer, as Figures 1-4 shown, including a micro flow cytometer body 1. A detection cavity 15 is formed in the outer wall of one side of the micro flow cytometer body 1. A rotary cylinder 2 is fixedly connected to the center of the inner bottom of the detection cavity 15 by bolts. The output end of the rotary cylinder 2 rotates 90 degrees each time. The rotary cylinder 2 is fixedly connected to a turntable 3 through its output end on one side. A test tube slot 4 is formed in the outer wall of one side of the turntable 3. A first support plate 5 and a second support plate 6 are fixedly connected to the outer wall of the top of the turntable 3 near the outside of the test tube slot 4 by bolts. The first support plate 5 is located on one side of the second support plate 6. Micro electric push rods 7 are fixedly connected to the outer walls of the opposite sides of the first support plate 5 and the second support plate 6 by bolts. The micro electric push rods 7 are fixedly connected to test tube clamping pallets 8 through their output ends on one side. A telescopic cylinder 9 is fixedly connected to the inner bottom of the detection cavity 15 near one side of the rotary cylinder 2 by bolts. The telescopic cylinder 9 is fixedly connected to a lifting platform 10 through its output end on one side. A motor 11 is fixedly connected to the center of the outer wall of one side of the lifting platform 10 by bolts. The motor 11 is fixedly connected to a ball screw 12 through its output end on one side. A through groove for the rotation of the ball screw 12 is formed in the top of the lifting platform 10. A nut seat on the ball screw 12 is fixedly connected to a moving plate 13 by screws. A test tube sleeve 14 is arranged on the top of the moving plate 13.

[0022] It should be noted that since the existing micro flow cytometer requires the tester to replace the test tube after the detection is completed, and the instrument needs to be in a standby state when replacing the test tube, the working efficiency of the micro flow cytometer is low. In this embodiment, the test tube is clamped and lifted by the test tube clamping pallet 8, which can ensure the stability of the test tube during collection, and the test tube is lifted up and down by the test tube sleeve 14, so that the micro flow cytometer body 1 can realize the loading and unloading of the test tube during the working state, realize the automatic replacement of the test tube, not only ensure the working efficiency of the micro flow cytometer, but also do not require manual replacement, saving manpower and avoiding the problem of pollution caused by the spillage of the collection liquid in the test tube.

[0023] Specifically, in this embodiment, the solution mainly includes a turntable 3 and a lifting table 10. When in use, an empty test tube is inserted into a test tube sleeve 14 on one of the lifting tables 10. When loading the test tube, the motor 11 is controlled to drive the ball screw 12 to rotate through the output end on one side thereof. The moving plate 13 on the ball screw 12 moves on the lifting table 10, and the test tube sleeve 14 and the test tube on the lifting table 10 move synchronously. The test tube inserted into the test tube sleeve 14 moves towards the test tube slot 4 and moves into it. At this time, the micro electric push rod 7 is controlled to move. The micro electric push rod 7 drives the test tube clamping support plate 8 to move through the output end on one side thereof. The two test tube clamping support plates 8 move towards each other to clamp and lift the test tube. After the test tube is clamped, the rotary cylinder 2 drives the turntable 3 to rotate 90 degrees through the output end on one side thereof. The test tube on the turntable 3 moves to directly below the detection outlet of the micro flow cytometer body 1, and the test tube collects the cell solution. After the test tube is collected, the rotary cylinder 2 drives the turntable 3 to rotate 90 degrees again through the output end on one side thereof. When the test tube moves above the other lifting table 10, the test tube can fall into the inserted test tube sleeve 14 by moving the two test tube clamping support plates 8 in opposite directions, realizing test tube unloading. The working intervals and working sequences of the rotary cylinder 2, the micro electric push rod 7, the telescopic cylinder 9, and the motor 11 can be programmed and controlled by a PLC controller.

[0024] In a further preferred embodiment of the present invention, as Figure 2 shown, four test tube slots 4 are provided, and the four test tube slots 4 are symmetrically opened on the outer side wall of the turntable 3. A groove with a semi-circular cross-section is opened on the inner side wall of the test tube slot 4.

[0025] In this embodiment, through the setting of the four test tube slots 4, when one test tube is collecting cell solution, one test tube is in the loading state, and another test tube is in the unloading state.

[0026] In a further preferred embodiment of the present invention, as Figure 2 and Figure 4 shown, the cross-section of the test tube clamping support plate 8 is a semi-circular ring structure.

[0027] In this embodiment, the two test tube clamping support plates 8 with semi-circular ring structures can clamp and lift the test tube.

[0028] In a further preferred embodiment of the present invention, as Figure 1 shown, four telescopic cylinders 9 are provided, and every two of the four telescopic cylinders 9 form a group. The two groups of telescopic cylinders 9 are arranged at a 90-degree angle at the inner bottom of the detection cavity 15.

[0029] In this embodiment, through the setting of the two lifting tables 10, test tube loading and test tube unloading can be realized simultaneously. The micro flow cytometer body 1 can realize test tube replacement without standby, and the efficiency is high.

[0030] It should be noted that, for the foregoing embodiments, for the sake of simple description, they are all expressed as a series of action combinations. However, those skilled in the art should be aware that the present utility model is not limited by the described action sequence, because according to the present utility model, certain steps may be performed in other sequences or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily essential to the present utility model.

[0031] In several embodiments provided by the present application, it should be understood that the disclosed device can be implemented in other ways. For example, the device embodiments described above are merely illustrative. For example, the above-mentioned unit division may have other division methods in actual implementation. For example, multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the shown or discussed coupling or communication connection between each other can be through some interfaces. The indirect coupling or communication connection between devices or units can be in the form of telecommunications or other forms.

[0032] The units described above as separate components may or may not be physically separated. The components shown as units may or may not be physical units, that is, they can be located in one place, or they can be distributed to multiple network units. Some or all of the units can be selected according to actual needs to achieve the purpose of the solution of this embodiment.

[0033] The above embodiments are only used to illustrate the technical solutions of the present utility model, rather than limiting the protection scope of the utility model. Obviously, the described embodiments are only some embodiments of the present utility model, rather than all embodiments. Based on these embodiments, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model. Although the present utility model has been described in detail with reference to the above embodiments, those of ordinary skill in the art can still, without conflict, make combinations, additions, deletions or other adjustments to the features in the embodiments of the present utility model according to the situation without creative efforts, so as to obtain different technical solutions that essentially do not deviate from the concept of the present utility model. These technical solutions also belong to the scope of protection of the present utility model.

Claims

1. A micro flow cytometer, characterized in that, Including: Miniature flow cytometer body (1); Detection chamber (15) opened on the outer wall of the miniature flow cytometer body (1); Rotary cylinder (2) fixed to the central position at the inner bottom of the detection chamber (15) by bolts; Turntable (3) fixed to the output end of the rotary cylinder (2); Test tube card slots (4) opened on the outer wall of the turntable (3); and First support plate (5) and second support plate (6) fixed to the top of the turntable (3) near the outer side of the test tube card slot (4) by bolts, the first support plate (5) is located on one side of the second support plate (6); Miniature electric push rods (7) both fixed to the outer walls of the first support plate (5) and the second support plate (6) by bolts; Test tube clamping support plate (8) fixed to the output end of the miniature electric push rod (7); Retractable cylinder (9) fixed to the inner bottom of the detection chamber (15) near one side of the rotary cylinder (2) by bolts; Lifting platform (10) fixed to the output end of the retractable cylinder (9); Motor (11) fixed to the central position of the outer wall of the lifting platform (10) by bolts; Ball screw (12) fixed to the output end of the motor (11); Moving plate (13) fixed to the nut seat on the ball screw (12) by screws; Test tube sleeve (14) provided on the top of the moving plate (13).

2. The micro flow cytometer according to claim 1, wherein There are four test tube card slots (4) in total, and the four test tube card slots (4) are symmetrically opened on the outer wall of the turntable (3), and a groove with a semi-circular cross-section is opened on the inner wall of the test tube card slot (4).

3. The micro flow cytometer according to claim 1, characterized in that, The cross-section of the test tube clamping support plate (8) is a semi-circular ring structure.

4. A micro flow cytometer according to claim 1, characterized in that, There are four retractable cylinders (9) in total, and every two of the four retractable cylinders (9) form a group, and the two groups of retractable cylinders (9) are arranged at a 90-degree angle at the inner bottom of the detection chamber (15).

5. A micro flow cytometer according to claim 1, characterized in that, A through groove for the rotation of the ball screw (12) is opened on the top of the lifting platform (10).

6. The micro flow cytometer according to claim 1, wherein, The rotation angle of the output end of the rotary cylinder (2) is 90 degrees each time.

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