Purifying device for food enzyme preparation

Abstract

The utility model discloses a food enzyme preparation purification device, which relates to the technical field of purification devices, and comprises a shell, the top surface of the shell is provided with a feed port penetrating through the top surface of the shell, the top surface of the feed port is provided with a sealing cover, the bottom surface of the feed port is connected with a dialysis bag, and the bottom end of the outer wall of the feed port is connected with an inner limiting shell. A rotating motor is installed on the bottom face of the inner wall of the shell, the output end of the top face of the rotating motor is connected with an outer limiting shell, the outer limiting shell is matched with the inner limiting shell, the inner wall, located inside, of the outer limiting shell is connected with a plurality of scraping plates, and the edge, facing the middle, of each scraping plate is matched with the dialysis bag. The scraping plate rotates around the dialysis bag, crystals on the surface of the dialysis bag are scraped off, a buffer solution and a mixed solution in the dialysis bag are driven to shake, and the purification efficiency of a food enzyme preparation is improved; along with rotation of the outer limiting shell, crystals are left in the containing groove, the purity of the buffer solution is recovered, and recycling of the buffer solution is achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of purification devices, and specifically relates to a purification device for food enzyme preparations. Background Art

[0002] Enzyme preparations are important raw materials widely used in fields such as food, biomedicine, etc. Immobilized enzymes and immobilized cells are also a form of enzyme preparations and are widely used in food industry, medicine, chemical industry, textile, environmental protection, energy, etc. And enzyme preparations are purified enzymes obtained through large-scale fermentation and purification. Compared with enzymes in the natural state, enzyme preparations usually have the following characteristics: high purity, enzyme preparations usually go through precise separation and purification processes, so they have high purity and activity; stability, enzyme preparations usually have high stability during the catalytic process and can maintain their catalytic activity for a long time; specificity, enzyme preparations have high specificity for specific substrates and will not randomly catalyze other reactions; adaptability, enzyme preparations can work within a relatively wide range of temperatures and pH values; biocompatibility, enzyme preparations usually have good biocompatibility with organisms and are suitable for use in the pharmaceutical and food industries.

[0003] Equilibrium dialysis is one of the commonly used methods for purifying enzyme preparations. It uses the differences in the size and charge of molecules to separate the components in a mixture. Equilibrium dialysis utilizes the characteristics of a semi-permeable membrane to separate the mixture from the buffer solution and controls the transport of substances through the pores or channels on the semi-permeable membrane. In this process, small molecule solutes, etc. can diffuse through the semi-permeable membrane into the buffer solution, while macromolecules are restricted inside the dialysis bag.

[0004] It is found in actual use that the equipment for purifying enzyme preparations using equilibrium dialysis on the current market has the problem that the buffer solution is relatively static, and the crystals dialyzed out linger near the micropores of the dialysis bag, causing a large area of blockage of the dialysis micropores of the dialysis bag, resulting in a reduction in the dialysis purification efficiency. And due to the presence of crystals, the purity of the buffer solution is reduced, further causing a reduction in the dialysis purification efficiency, and the buffer solution containing crystals cannot be directly recycled. Content of the Utility Model

[0005] Based on this, the purpose of the present utility model is to provide a purification device for food enzyme preparations to solve the technical problems of low purification efficiency of traditional devices and the inability to recycle the buffer solution.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a device for purifying food enzyme preparations, comprising an outer shell, a feed port penetrating the top surface of the outer shell is provided on the top surface of the outer shell, and a sealing cover is provided on the top surface of the feed port, a dialysis bag is connected to the bottom surface of the feed port, an inner limit shell is connected to the bottom end of the outer wall of the feed port, a rotating motor is installed on the bottom surface of the inner wall of the outer shell, and an outer limit shell is connected to the output end of the top surface of the rotating motor, the outer limit shell cooperates with the inner limit shell, a plurality of scrapers are connected to the inner wall of the outer limit shell, and the edge of each scraper facing the middle cooperates with the dialysis bag.

[0007] By adopting the above technical solution, the rotating motor drives the scraper in the outer limiting shell to rotate around the dialysis bag, scraping off the crystals on the surface of the dialysis bag, and driving the buffer solution and the mixed liquid in the dialysis bag to shake, thereby accelerating the purification efficiency of the food enzyme preparation.

[0008] The utility model is further configured such that a drainage groove is opened in the external gap between the outer limit shell and the inner limit shell, and the end of the drainage groove facing the center is located at the location where the internal radius of the outer limit shell and the inner limit shell is the largest, the drainage groove is an inverted cone, and a storage block is threaded through the top surface of the outer shell and is in sliding contact with the inner wall of the drainage groove away from the center end, and the bottom end of the storage block is connected to a C-shaped storage groove located in the drainage groove, and the surface of the storage groove has tiny filtering holes.

[0009] By adopting the above technical solution, the crystals in the buffer solution enter the drainage groove under the action of centrifugal force as the outer limiting shell rotates, and continuously rotates outward in the drainage groove until it approaches the edge of the storage groove. At this time, the crystals carried by the rotating buffer solution are transferred into the drainage groove, and the buffer solution continues to rotate, and the crystals are retained in the storage groove until use is completed, when the storage block is rotated to separate the storage block from the outer shell, and the crystals in the storage groove are cleaned, thereby realizing the recycling of the buffer solution.

[0010] The utility model is further configured as follows: the bottom end of the dialysis bag is connected to a discharge block, and the inner part of the outer wall of the discharge block is slidably sleeved with the outer limit shell, the bottom end of the discharge block is installed with a flow valve, and the flow valve is installed on the inner wall of the outer shell, the bottom end of the connection between the flow valve and the discharge block is connected to a discharge pipe, and the discharge pipe passes through the outer wall of the outer shell.

[0011] By adopting the above technical solution, the fixed discharge block can prevent the dialysis bag from rotating or shaking randomly with the rotation of the scraper during use, thereby preventing the dialysis bag from being damaged during use and extending its service life. After purification is completed, the circulation valve is opened and the purified food enzyme preparation is discharged along the discharge pipe.

[0012] In summary, the utility model mainly has the following beneficial effects:

[0013] The utility model rotates a scraper around a dialysis bag to scrape off the crystals on the surface of the dialysis bag, and drives the buffer solution and the mixed solution in the dialysis bag to shake, so as to improve the purification efficiency of the food enzyme preparation; as the outer limiting shell rotates, under the action of centrifugal force, the crystals are left in the storage tank, the purity of the buffer solution is restored, and the recycling of the buffer solution is realized. Brief Description of the Drawings

[0014] Figure 1 is a schematic diagram of the overall structure of the utility model;

[0015] Figure 2 is a front sectional view of the overall structure of the utility model;

[0016] Figure 3 is a side sectional view of the overall structure of the utility model;

[0017] Figure 4 is a first top sectional view of the overall structure of the utility model;

[0018] Figure 5 is a second top sectional view of the overall structure of the utility model.

[0019] In the figure: 1, outer shell; 2, dialysis bag; 3, inner limiting shell; 4, outer limiting shell; 5, drainage groove; 6, sealing cover; 7, feed inlet; 8, discharge block; 9, flow valve; 10, discharge pipe; 11, scraper; 12, storage tank; 13, storage block; 14, rotating motor. Detailed Description of the Preferred Embodiments

[0020] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. The embodiments described below with reference to the accompanying drawings are exemplary only for explaining the present utility model and should not be construed as limiting the present utility model.

[0021] Next, the embodiments of the present utility model will be described according to the overall structure of the present utility model.

[0022] A purification device for food enzyme preparations, such as Figures 1-5As shown in the figure, it includes a housing 1. There is a feed inlet 7 penetrating through the top surface of the housing 1 on the top surface of the housing 1, and a sealing cover 6 is arranged on the top surface of the feed inlet 7. A dialysis bag 2 is connected to the bottom surface of the feed inlet 7. The bottom end of the outer wall of the feed inlet 7 is connected with an inner limiting shell 3. A rotating motor 14 is installed on the bottom surface of the inner wall of the housing 1, and the output end on the top surface of the rotating motor 14 is connected with an outer limiting shell 4. The output end of the rotating motor 14 is connected with a gear, and a toothed ring is connected to the bottom end of the outer limiting block 4. The gear meshes with the toothed ring to drive the outer limiting shell 4 to rotate. The outer limiting shell 4 cooperates with the inner limiting shell 3. The inner wall of the outer limiting shell 4 located inside is connected with a plurality of scraping plates 11, and the edge of each scraping plate 11 facing the middle cooperates with the dialysis bag 2. The rotating motor 14 drives the scraping plates 11 in the outer limiting shell 4 to rotate around the dialysis bag 2, scraping the crystals on the surface of the dialysis bag 2, and driving the buffer solution and the mixed solution in the dialysis bag 2 to shake, accelerating the purification efficiency of the food enzyme preparation.

[0023] A drainage groove 5 is arranged at the external gap between the outer limiting shell 4 and the inner limiting shell 3, and one end of the drainage groove 5 facing the center is located at the position with the largest internal radius dimension of the outer limiting shell 4 and the inner limiting shell 3. The drainage groove 5 is in an inverted conical shape. A receiving block 13 is threaded through the top surface of the housing 1 and is in sliding contact with the inner wall of the end of the drainage groove 5 far from the center. The bottom end of the receiving block 13 is connected with a C-shaped receiving groove 12 located in the drainage groove 5. The surface of the receiving groove 12 has tiny filter holes. As the outer limiting shell 4 rotates, the crystals in the buffer solution enter the drainage groove 5 under the action of centrifugal force and continuously rotate outward in the drainage groove 5 until they approach the edge of the receiving groove 12. At this time, the rotating buffer solution with the crystals is transferred into the drainage groove 5. The buffer solution continues to rotate, and the crystals are left in the receiving groove 12. Until after use, rotate the receiving block 13 to make the receiving block 13 separate from the housing 1, and clean the crystals in the receiving groove 12 to realize the recycling of the buffer solution.

[0024] The bottom end of the dialysis bag 2 is connected with a discharge block 8, and the inner part of the outer wall of the discharge block 8 is slidably sleeved with the outer limiting shell 4. A flow valve 9 is installed at the bottom end of the discharge block 8, and the flow valve 9 is installed on the inner wall of the housing 1. The bottom end of the connection between the flow valve 9 and the discharge block 8 is connected with a discharge pipe 10, and the discharge pipe 10 penetrates through the outer wall of the housing 1. The fixation of the discharge block 8 can prevent the dialysis bag 2 from rotating or swaying randomly with the rotation of the scraping plate 11 during use, prevent the dialysis bag 2 from being damaged during use, and extend the service life. After the purification is completed, open the flow valve 9, and the purified food enzyme preparation is discharged along the discharge pipe 10.

[0025] Working principle: The circulation valve 9 is in a closed state. The food enzyme preparation to be purified is poured into the dialysis bag 2 through the feed port 7, and the sealing cover 6 is covered. The storage block 13 on the top surface of the outer shell 1 is rotated and opened, and then the buffer solution is poured into the space formed by the outer limiting shell 4 and the inner limiting shell 3 through the drainage groove 5. The storage block 13 is rotated and closed. The rotating motor 14 drives the scraper 11 in the outer limiting shell 4 to rotate around the dialysis bag 2, scraping off the crystals on the surface of the dialysis bag 2, and driving the buffer solution and the mixed solution in the dialysis bag 2 to shake, accelerating the purification efficiency of the food enzyme preparation. The crystals in the buffer solution enter the drainage groove 5 under the action of centrifugal force as the outer limiting shell 4 rotates, and continuously rotate outward in the drainage groove 5 until they approach the edge of the storage groove 12. At this time, the rotating buffer solution carries the crystals through the opening of the drainage groove 5 into the drainage groove 5. The buffer solution continues to rotate and is discharged through the microfiltration holes of the drainage groove 5, while the crystals are left in the storage groove 12. Until after use, the storage block 13 is rotated to make the storage block 13 separate from the outer shell 1, and the crystals in the storage groove 12 are cleaned, realizing the recycling of the buffer solution. The fixation of the discharge block 8 can prevent the dialysis bag 2 from rotating or shaking randomly as the scraper 11 rotates during use, preventing the dialysis bag 2 from being damaged during use and extending its service life. After the purification is completed, the circulation valve 9 is opened, and the purified food enzyme preparation is discharged along the discharge pipe 10.

[0026] Based on the above structure, in this embodiment, although the embodiments of the present invention have been shown and described, this specific embodiment is only an explanation of the present invention, and it is not a limitation of the invention. The specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art can make modifications, substitutions and variations that do not contribute creatively to the embodiments according to needs, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. A purification device for a food enzyme preparation, comprising a housing (1), characterized in that: The top surface of the outer shell (1) is provided with a feed inlet (7) penetrating through the top surface of the outer shell (1), and a sealing cover (6) is arranged on the top surface of the feed inlet (7). The bottom surface of the feed inlet (7) is connected with a dialysis bag (2). The bottom end of the outer wall of the feed inlet (7) is connected with an inner limiting shell (3). The bottom surface of the inner wall of the outer shell (1) is provided with a rotating motor (14), and the output end of the top surface of the rotating motor (14) is connected with an outer limiting shell (4). The outer limiting shell (4) is matched with the inner limiting shell (3). The inner wall of the outer limiting shell (4) located inside is connected with a plurality of scraping plates (11), and the edges of each scraping plate (11) facing the middle are all matched with the dialysis bag (2).

2. The purification device for a food enzyme preparation according to claim 1, characterized in that: A drainage groove (5) is formed at the external gap between the outer limiting shell (4) and the inner limiting shell (3), and one end of the drainage groove (5) facing the center is located at the position where the inner radius dimension of the outer limiting shell (4) and the inner limiting shell (3) is the largest.

3. The purification device for a food enzyme preparation according to claim 2, characterized in that: The drainage groove (5) is in an inverted conical shape. The top surface of the outer shell (1) is threadedly penetrated with a storage block (13) which is in sliding contact with the inner wall of the end of the drainage groove (5) far from the center. The bottom end of the storage block (13) is connected with a C-shaped storage groove (12) located in the drainage groove (5). The surface of the storage groove (12) has tiny filter holes.

4. A purification device for a food enzyme preparation according to claim 1, wherein: The bottom end of the dialysis bag (2) is connected with a discharge block (8), and the inner part of the outer wall of the discharge block (8) is slidably sleeved with the outer limiting shell (4). The bottom end of the discharge block (8) is provided with a flow valve (9), and the flow valve (9) is installed on the inner wall of the outer shell (1).

5. The purification device for a food enzyme preparation according to claim 4, wherein: The bottom end of the connection between the flow valve (9) and the discharge block (8) is connected with a discharge pipe (10), and the discharge pipe (10) penetrates through the outer wall of the outer shell (1).

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