Cell culture device capable of avoiding heat loss

Abstract

The utility model discloses a cell culture device capable of avoiding heat loss, which belongs to the technical field of cell culture devices and comprises a base, a rotating seat is rotatably connected to the middle of the top of the base, a glass cover is fixedly connected to the middle of the top of the rotating seat, and a fixing ring is fixedly connected to the top of the glass cover. A cover body is in threaded connection with the outer side wall of the fixing ring, a cylinder is fixedly connected to the middle of the top of the inner side wall of the cover body, and the bottom of the cylinder extends to the inner side of the glass cover. The culture dish has the advantages that heat loss caused by frequent taking of the culture dish is avoided, accordingly, the cell culture effect is improved, the glass cover can be shielded or opened through the cover body according to the condition that cell sap cultured in the culture dish needs or does not need illumination, and accordingly the overall applicability is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture devices, and specifically relates to a cell culture device that can avoid heat loss. Background Technique

[0002] Cell culture refers to a method of simulating the in-vivo environment aseptically, at an appropriate temperature, pH value, and certain nutrient conditions in vitro, so that cells can survive, grow, reproduce, and maintain their main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology, which is usually carried out using a cell culture device.

[0003] When using a cell culture device to culture cells, it is necessary to monitor the cell culture status. When it is found that the cell liquid in the culture dish has sediment, the culture dish needs to be taken out and shaken to mix the cell liquid evenly. However, the existing cell culture devices are not convenient for monitoring and shaking the culture dish. It is necessary to open the cell culture device, take out the culture dish for observation, and then perform the shaking and mixing operation. Since the temperature inside the cell culture device is constant, frequent opening and closing of the cell culture device will cause heat loss inside the cell culture device, which will also have a certain impact on the cell culture effect. Therefore, we propose a cell culture device that can avoid heat loss. Content of the Utility Model

[0004] The purpose of the utility model is to provide a cell culture device that can avoid heat loss, so as to solve the problems in the above background technique that the existing cell culture devices are not convenient for monitoring and shaking the culture dish. It is necessary to open the cell culture device, take out the culture dish for observation, and then perform the shaking and mixing operation. Since the temperature inside the cell culture device is constant, frequent opening and closing of the cell culture device will cause heat loss inside the cell culture device, which will also have a certain impact on the cell culture effect.

[0005] To achieve the above object, the utility model provides the following technical solution: A cell culture device that can avoid heat dissipation, including a base, a rotating seat is rotatably connected to the middle of the top of the base, a glass cover is fixedly connected to the middle of the top of the rotating seat, a fixing ring is fixedly connected to the top of the glass cover, a cover body is screwed to the outer side wall of the fixing ring, a cylinder is fixedly connected to the middle of the top inner side wall of the cover body, the bottom of the cylinder extends to the inner side of the glass cover, notch openings are formed on the front and rear sides and the upper sides of the left and right sides of the outer side wall of the cylinder, a connecting block is slidably connected to the inner cavity wall of the notch opening, one side of the connecting block extends to the inner cavity of the cylinder and is fixedly connected to a pull rod, the top ends of the four pull rods extend to the outer side of the cover body, the other side of the connecting block extends to the outer side of the cylinder and is fixedly connected to a placement ring, rubber fixing blocks are fixedly connected to the front and rear sides and the left and right sides of the inner cavity wall of the placement ring, a culture dish is clamped between the inner side walls of the four rubber fixing blocks, and a heating plate is fixedly connected to the top of the rotating seat and located inside the glass cover.

[0006] As a further description of the above technical solution:

[0007] A cavity is formed inside the glass cover, and the cavity is in a vacuum state.

[0008] As a further description of the above technical solution:

[0009] The outer side wall of the glass cover is coated with nano transparent heat insulation paint.

[0010] As a further description of the above technical solution:

[0011] Sliding connections are provided between the left and right sides of the outer side wall of the glass cover, between the top of the rotating seat and the bottom of the cover body, and the right side wall of the left cover body is in contact with the left side wall of the right cover body.

[0012] As a further description of the above technical solution:

[0013] A chute is formed in the middle of the inside of the base, square rods are slidably connected to the left and right sides of the inner cavity wall of the chute, the ends of the square rods extend to the outer side of the base, a damping rotating shaft is fixedly connected to the top of the square rods and located outside the base, the top end of the damping rotating shaft is fixedly connected to an L-shaped rod, and the end of the L-shaped rod is fixedly connected to the outer side wall of the cover body.

[0014] As a further description of the above technical solution:

[0015] Fixing grooves are formed on the front and rear sides of the right side wall of the left cover body, rubber insertion blocks are fixedly connected to the front and rear sides of the left side wall of the right cover body, and the ends of the rubber insertion blocks extend into the inner cavity of the fixing grooves and are clamped with the inner cavity wall of the fixing grooves.

[0016] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0017] 1. For the cell culture device capable of avoiding heat dissipation, the cell liquid inside the culture dish is monitored through the rotating seat and the glass cover. The cell liquid inside the culture dish is shaken evenly through the pull rod, connecting block, notch, placing ring and rubber fixing block. Thus, there is no need to frequently take the culture dish. The glass cover is thermally insulated inside through the cavity and the nano transparent heat insulation coating to further avoid the temperature loss inside the glass cover, which can facilitate the monitoring and shaking of the cell liquid in the culture dish, avoid the heat loss inside caused by frequent taking of the culture dish, and thus improve the effect of cell culture.

[0018] 2. For the cell culture device capable of avoiding heat dissipation, when illumination is required, two L-shaped rods are pulled to both sides. The L-shaped rods cooperate with the square rod and the chute through the damping rotating shaft to pull the two cover bodies to both sides, so that the cover bodies are separated from the glass cover, enabling the glass cover to receive illumination. When illumination is not required, the two cover bodies are covered outside the glass cover, and then the two cover bodies are fixed by inserting the rubber plug into the fixing groove. It can block or open the glass cover through the cover body according to whether the cell liquid cultured in the culture dish requires illumination conditions, thereby improving the overall applicability. Description of the Drawings

[0019] Figure 1 is a three-dimensional structural schematic diagram of a cell culture device capable of avoiding heat dissipation proposed by the present utility model;

[0020] Figure 2 is a front sectional view of the structure of a cell culture device capable of avoiding heat dissipation proposed by the present utility model;

[0021] Figure 3 is a top view of the structure of a rubber fixing block of a cell culture device capable of avoiding heat dissipation proposed by the present utility model;

[0022] Figure 4 is a front view of the structure of a front rubber plug of a cell culture device capable of avoiding heat dissipation proposed by the present utility model.

[0023] In the figure: 100, base; 110, rotating seat; 111, glass cover; 112, fixing ring; 113, cover body; 114, cylinder; 115, notch; 116, connecting block; 117, pull rod; 118, placing ring; 119, rubber fixing block; 120, culture dish; 121, heating plate; 130, cavity; 140, cover body; 150, chute; 151, square rod; 152, damping rotating shaft; 153, L-shaped rod; 160, fixing groove; 161, rubber plug. Detailed implementation mode

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative work shall fall within the protection scope of the present invention.

[0025] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise stated, the meaning of "a plurality" is two or more.

[0026] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0027] The present invention provides a cell culture device that can avoid heat dissipation, which can conveniently monitor and shake the cell liquid in the culture dish 120 evenly, avoid heat dissipation inside caused by frequent taking of the culture dish 120, thereby improving the effect of cell culture, and can block or open the glass cover 111 through the cover body 140 according to whether the cell liquid cultured in the culture dish 120 needs light conditions, thereby improving the overall applicability. Please refer to Figures 1-4 , including a base 100;

[0028] Please refer to again Figures 1-4, a rotating seat 110 is rotatably connected to the middle of the top of the base 100. The rotating seat 110 is used to drive the glass cover 111 to rotate for facilitating the monitoring of the culture dish 120. A glass cover 111 is fixedly connected to the middle of the top of the rotating seat 110. The glass cover 111 is used for facilitating the observation of the culture dish 120. A fixing ring 112 is fixedly connected to the top of the glass cover 111. The fixing ring 112 is used for installing the cover body 113. The cover body 113 is screwed on the outer side wall of the fixing ring 112. The cover body 113 is used for installing the cylinder 114. A cylinder 114 is fixedly connected to the middle of the top of the inner side wall of the cover body 113. The cylinder 114 is used for installing the connecting block 116 through the notch 115. The bottom of the cylinder 114 extends to the inside of the glass cover 111. Notches 115 are opened on the front and rear sides and the upper sides of the left and right sides of the outer side wall of the cylinder 114. The notch 115 is used for limiting the connecting block 116. A connecting block 116 is slidably connected to the inner cavity wall of the notch 115. The connecting block 116 is used for connecting the pull rod 117 and the placing ring 118. One side of the connecting block 116 extends into the inner cavity of the cylinder 114 and is fixedly connected to the pull rod 117. The pull rod 117 is used for driving the placing ring 118 to move up and down through the connecting block 116. The tops of the four pull rods 117 extend to the outside of the cover body 113. The other side of the connecting block 116 extends to the outside of the cylinder 114 and is fixedly connected to the placing ring 118. The placing ring 118 is used for cooperating with the rubber fixing block 119 to fix the culture dish 120. Rubber fixing blocks 119 are fixedly connected to the front and rear sides and the left and right sides of the inner cavity wall of the placing ring 118. The rubber fixing block 119 is used for fixing the culture dish 120 by its own elasticity. The culture dish 120 is clamped between the inner side walls of the four rubber fixing blocks 119. The culture dish 120 is used for placing cell liquid. A heating plate 121 is fixedly connected to the top of the rotating seat 110 and inside the glass cover 111. The heating plate 121 is used for keeping a certain temperature inside the glass cover 111. A cavity 130 is opened inside the glass cover 111. The cavity 130 is used for preventing heat from being lost through convection and conduction, so as to keep the temperature inside the glass cover 111. The cavity 130 is in a vacuum state. The outer side wall of the glass cover 111 is coated with a nano transparent heat insulation coating. The nano transparent heat insulation coating is used for effectively isolating the influence of the heat outside the glass cover 111 on the inside, and further keeping the temperature inside the glass cover 111. By inserting the culture dish 120 between the rubber fixing blocks 119 in the placing ring 118, the culture dish 120 is fixed by the elasticity of the rubber fixing block 119. Place the cylinder 114 into the glass cover 111 and connect the cover body 113 to the fixing ring 112 by rotating the cover body 113, so as to fix the culture dish 120 in the glass cover 111. Start the heating plate 121 through an external controller to heat the inside of the glass cover 111. The glass cover 111 cooperates with the cavity 130 and the nano transparent heat insulation coating to keep the temperature inside the glass cover 111 and prevent the temperature inside the glass cover 111 from being lost.The cell fluid inside the culture dish 120 is monitored by rotating the rotating base 110 in cooperation with the glass cover 111. When sedimentation occurs in the cell fluid inside the culture dish 120, the pull rod 117 is reciprocated up and down. The pull rod 117 drives the placement ring 118 to move up and down through the connecting block 116 and the notch 115, thereby shaking and mixing the cell fluid inside the culture dish 120 evenly. This way, there is no need to take out the culture dish 120, avoiding heat loss.

[0029] In summary, it is convenient to monitor and shake the cell fluid in the culture dish 120 evenly, avoiding heat loss inside caused by frequent removal of the culture dish 120, thus improving the effect of cell culture.

[0030] Please refer to again Figures 1-4 On the left and right sides of the outer wall of the glass cover 111, between the top of the rotating base 110 and the bottom of the cover body 113, a cover body 140 is slidably connected. The cover body 140 is used to block or open the glass cover 111 according to whether the cell fluid cultured in the culture dish 120 requires light conditions. The right side wall of the left cover body 140 contacts the left side wall of the right cover body 140. In the middle of the interior of the base 100, a chute 150 is opened. The chute 150 is used to install a square rod 151. On the left and right sides of the inner cavity wall of the chute 150, a square rod 151 is slidably connected. The square rod 151 is used to install a damping rotating shaft 152. The end of the square rod 151 extends to the outside of the base 100. At the top of the square rod 151 and outside the base 100, a damping rotating shaft 152 is fixedly connected. The damping rotating shaft 152 is used to drive the L-shaped rod 153 to drive the cover body 140 to rotate. When light is needed, the cover body 140 is rotated to both sides to avoid blocking the glass cover 111, and at the same time, to prevent the cover body 111 from rotating itself. The top end of the damping rotating shaft 152 is fixedly connected to an L-shaped rod 153. The L-shaped rod 153 is used to drive the cover body 140 to move. The end of the L-shaped rod 153 is fixedly connected to the outer wall of the cover body 140. On the front and back sides of the right side wall of the left cover body 140, fixing grooves 160 are opened. The fixing grooves 160 are used to install rubber inserts 161. On the front and back sides of the left side wall of the right cover body 140, rubber inserts 161 are fixedly connected. The rubber inserts 161 are used to connect and fix the two cover bodies 140 by using their own elasticity in cooperation with the fixing grooves 160. The end of the rubber insert 161 extends into the inner cavity of the fixing groove 160 and is clamped with the inner cavity wall of the fixing groove 160. When light is needed, by pulling the two L-shaped rods 153 to both sides, the L-shaped rods 153 drive the two cover bodies 140 to be pulled to both sides through the damping rotating shaft 152, the square rod 151 and the chute 150, so that the cover body 140 is separated from the glass cover 111, and then the cover body 140 is rotated away from the glass cover 111 through the damping rotating shaft 152, so that the glass cover 111 can be illuminated. When light is not needed, the above operation is reversed. The two cover bodies 140 are fixedly sleeved outside the glass cover 111 by inserting the rubber inserts 161 into the fixing grooves 160.

[0031] In summary, the cover 140 can block or open the glass cover 111 according to whether the cell liquid cultured in the culture dish 120 requires light conditions, thereby improving the overall applicability.

[0032] In specific use, a person skilled in the art inserts the culture dish 120 between the rubber fixing blocks 119 in the placing ring 118, and uses the self-elasticity of the rubber fixing blocks 119 to fix the culture dish 120. Place the cylinder 114 into the glass cover 111 and rotate the cover body 113 to connect the cover body 113 with the fixing ring 112, fix the culture dish 120 in the glass cover 111, start the heating plate 121 through an external controller to heat the inside of the glass cover 111, and the glass cover 111 cooperates with the cavity 130 and the nano transparent heat insulation coating to keep the inside of the glass cover 111 warm and prevent the temperature inside the glass cover 111 from dissipating. Rotate the rotating base 110 to cooperate with the glass cover 111 to monitor the cell liquid inside the culture dish 120. When the cell liquid inside the culture dish 120 deposits, pull the pull rod 117 up and down reciprocally. The pull rod 117 cooperates with the connecting block 116 and the notch 115 to make the placing ring 118 move up and down reciprocally, thereby shaking and mixing the cell liquid inside the culture dish 120 evenly. When the culture dish 120 needs light, pull the two L-shaped rods 153 to both sides. The L-shaped rods 153 cooperate with the damping rotating shafts 152, the square rods 151 and the sliding grooves 150 to pull the two covers 140 to both sides, so that the covers 140 are separated from the glass cover 111, and then rotate the covers 140 away from the glass cover 111 through the damping rotating shafts 152, so that the glass cover 111 can be illuminated. When light is not required, reverse the above operations, so that the rubber insertion blocks 161 are inserted into the fixing grooves 160 to fix the two covers 140 on the outside of the glass cover 111.

[0033] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "schematic embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0034] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the claims and their equivalents.

Claims

1. A cell culture device capable of avoiding heat dissipation, characterized in that: It includes a base (100). A rotating seat (110) is rotatably connected to the middle of the top of the base (100). A glass cover (111) is fixedly connected to the middle of the top of the rotating seat (110). A fixing ring (112) is fixedly connected to the top of the glass cover (111). A cover body (113) is screwed to the outer side wall of the fixing ring (112). A cylinder (114) is fixedly connected to the middle of the inner side wall of the top of the cover body (113). The bottom of the cylinder (114) extends to the inside of the glass cover (111). Notches (115) are formed on the front and back sides and the upper sides of the left and right sides of the outer side wall of the cylinder (114). A connecting block (116) is slidably connected to the inner cavity wall of the notch (115). One side of the connecting block (116) extends to the inner cavity of the cylinder (114) and is fixedly connected to a pull rod (117). The tops of the four pull rods (117) extend to the outside of the cover body (113). The other side of the connecting block (116) extends to the outside of the cylinder (114) and is fixedly connected to a placing ring (118). Rubber fixing blocks (119) are fixedly connected to the front and back sides and the left and right sides of the inner cavity wall of the placing ring (118). A culture dish (120) is clamped between the inner side walls of the four rubber fixing blocks (119). A heating plate (121) is fixedly connected to the top of the rotating seat (110) and inside the glass cover (111).

2. The cell culture device capable of avoiding heat dissipation according to claim 1, wherein: A cavity (130) is formed inside the glass cover (111), and the cavity (130) is in a vacuum state.

3. The cell culture device capable of avoiding heat dissipation according to claim 1, characterized in that: The outer side wall of the glass cover (111) is coated with a nano transparent heat insulation coating.

4. The cell culture device capable of avoiding heat dissipation according to claim 1, characterized in that: A cover body (140) is slidably connected between the top of the rotating seat (110) and the bottom of the cover body (113) on the left and right sides of the outer side wall of the glass cover (111). The right side wall of the left cover body (140) is in contact with the left side wall of the right cover body (140).

5. The cell culture device capable of avoiding heat dissipation according to claim 4, characterized in that: A sliding groove (150) is formed in the middle of the inside of the base (100). Square rods (151) are slidably connected to the left and right sides of the inner cavity wall of the sliding groove (150). The ends of the square rods (151) extend to the outside of the base (100). A damping rotating shaft (152) is fixedly connected to the top of the square rod (151) and outside the base (100). The top of the damping rotating shaft (152) is fixedly connected to an L-shaped rod (153). The end of the L-shaped rod (153) is fixedly connected to the outer side wall of the cover body (140).

6. The cell culture device capable of avoiding heat dissipation according to claim 5, wherein: Fixing grooves (160) are formed on the front and back sides of the right side wall of the left cover body (140). Rubber insertion blocks (161) are fixedly connected to the front and back sides of the left side wall of the right cover body (140). The ends of the rubber insertion blocks (161) extend to the inner cavity of the fixing groove (160) and are clamped with the inner cavity wall of the fixing groove (160).

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