Quantitative protein detection kit

Abstract

Relates to the field of detection equipment, and discloses a protein quantitative detection kit which comprises a kit body, a test tube rack arranged in the kit body and test tubes placed in the test tube rack, and the kit body comprises a bottom box and a top cover connected to the bottom box in a covering mode. A guide rod used for supporting the test tube rack is fixedly arranged in the bottom box, and the test tube rack further comprises a supporting unit arranged at the lower end of the top cover and used for extruding the test tubes placed at the upper end of the test tube rack; the fixing units are arranged on the two sides of the top cover in a penetrating mode and used for fixing the top cover and the guide rods, the top of a test tube can be supported, the extrusion force of the supporting units on the test tube is small, and the test tube can be prevented from sliding on the test tube rack through friction force between the supporting units and the test tube; therefore, the test tube can be conveniently fixed, and the test tube is not easy to deform.

Description

Technical Field

[0001] The utility model relates to the technical field of detection equipment, in particular to a protein quantitative detection kit. Background Art

[0002] A protein quantitative detection kit is a chemical kit specifically designed to determine the protein content. These kits are based on different chemical reaction principles, including the reduction reaction of proteins with copper ions under alkaline conditions, the alkaline staining reaction of amino acids such as tyrosine and histidine, the binding reaction between Coomassie Brilliant Blue G-250 and proteins, and the formation of a complex between peptide bonds in proteins and copper ions.

[0003] After retrieval, a Chinese patent with the publication number CN213913914U discloses a test tube rack for a fluorescence quantitative PCR instrument. The device includes a support plate, a movable plate, and a driving mechanism. A first through hole is vertically opened inside the support plate, and a slot is opened in the middle inside the support plate. The left end of the movable plate is inserted into the slot, and the right end of the movable plate is connected to the driving mechanism. The left side of the driving mechanism is locked to the support plate through bolts. The movement of the movable plate is controlled by the driving mechanism. The test tube passes through the first through hole and the second through hole. The driving mechanism controls the movement of the movable plate, so that the second through hole inside the movable plate moves. The test tube is clamped by the inner walls of the first through hole and the second through hole to fix the test tube.

[0004] The existing equipment has the following disadvantages: When fixing the test tube by misaligning the second through hole on the connecting plate with the first through hole, since the connecting plate will squeeze the test tube, and since the sample test tube is generally made of PE material, the test tube will be deformed, and the size of the test tube will change. When it becomes smaller, the misalignment distance that needs to occur between the connecting plate and the support plate needs to be changed, and the squeezing force of the connecting plate on the test tube that the motor needs to regulate needs to change. The operation method is relatively inconvenient. When regulating the squeezing force of the connecting plate on the test tube and the pressure is large, the sample inside the test tube will be squeezed out, affecting the quantitative detection effect of the DAN sample in the test tube.

[0005] Therefore, the present application now proposes a protein quantitative detection kit to solve the above problems. Summary of the Utility Model

[0006] The purpose of the present utility model is to provide a protein quantitative detection kit to solve the problem that when the size of the test tube changes and becomes smaller, the misalignment distance that needs to occur between the connecting plate and the support plate needs to be changed, and the squeezing force of the connecting plate on the test tube that the motor needs to regulate needs to change, and the operation method is relatively inconvenient.

[0007] To achieve the above object, the present utility model provides the following technical solutions: A protein quantitative detection kit, comprising a kit, a test tube rack disposed inside the kit, and test tubes placed in the test tube rack. The kit includes a bottom box and a top cover connected to the bottom box. A guiding rod for supporting the test tube rack is fixedly disposed inside the bottom box. The kit further includes:

[0008] A supporting unit, disposed at the lower end of the top cover, for extruding the test tubes placed at the upper end of the test tube rack;

[0009] A fixing unit, penetrating through both sides of the top cover, for fixing the top cover to the guiding rod.

[0010] Wherein, the supporting unit includes a first elastic member fixedly spaced at the lower end of the top cover and a support plate fixed to the lower end of the first elastic member. The support plate is adjustably disposed above the test tube rack for supporting and fixing the test tubes placed on the test tube rack.

[0011] Wherein, the fixing unit includes a support rod penetrating through the top cover, a pulling plate fixed to the outer side of the support rod outside the top cover, and a fixing plate fixed to the inner side of the support rod inside the top cover for fixing the support rod to the guiding rod. A through hole for the movement of the support rod is provided at the corresponding position of the top cover with respect to the support rod.

[0012] Wherein, a second elastic member is sleeved between the top cover and the fixing plate of the support rod.

[0013] Wherein, a support frame for supporting the test tube rack is fixedly disposed on the guiding rod, and the fixing plate is adjustably disposed below the support frame;

[0014] The top end of the fixing plate abuts against the lower end of the support frame.

[0015] Wherein, the test tube rack includes a main frame for placing the test tubes and side plates fixed to both sides of the main frame. The side plates penetrate through the guiding rod, and positioning holes for placing the guiding rod are provided at the corresponding positions of the side plates with respect to the guiding rod;

[0016] Moving grooves for the movement of the fixing plate are provided at the corresponding positions of the side plates with respect to the fixing plate;

[0017] First placement grooves for placing the test tubes are spaced apart at the upper end of the main frame;

[0018] Second placement grooves for placing the test tubes are spaced apart at the lower end of the main frame;

[0019] The first placement groove and the second placement groove have different diameter sizes and are arranged staggeredly.

[0020] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0021] In the present utility model, all components of the reagent kit are restored to room temperature, and then it is checked whether there is precipitation in the protein labeling buffer. If there is precipitation, the reagent can be incubated in a 37°C water bath and gently mixed until the precipitate is completely dissolved. The test tube rack is placed on the alignment rod for fixation, and then multiple test tubes are respectively placed into the test tube rack. The prepared working solution is dropped into the test tubes one by one. After the detection solution is completely dropped, the fixing unit is pulled outward, the top cover is covered on the bottom box, and then the supporting unit squeezes the test tubes placed on the test tube rack. Then the fixing unit is reset so that the fixing unit is snap-fitted and fixed with the alignment rod, thereby fixing the top cover and the bottom box, so that the supporting unit fixes the test tubes on the test tube rack, completing the fixation work of the test tubes. Then the reagents inside the test tubes are incubated in the dark, and then according to the operation instructions of the fluorescence spectrometer, the detection program is selected to measure the fluorescence signal value. By setting this device, the top of the test tube can be supported, and the squeezing force of the supporting unit on the test tube is small. The frictional force between the supporting unit and the test tube can prevent the test tube from sliding on the test tube rack, so that the test tube can be conveniently fixed, and the test tube is not easily deformed, which can improve the detection efficiency of protein quantitative detection. Description of the Drawings

[0022] Figure 1 is the schematic diagram of the main structure in an embodiment of the present utility model;

[0023] Figure 2 is the schematic diagram of the cross-sectional structure in an embodiment of the present utility model;

[0024] Figure 3 is the schematic diagram of the longitudinal-sectional structure in an embodiment of the present utility model;

[0025] Figure 4 is the schematic diagram of the internal structure of the bottom box in an embodiment of the present utility model;

[0026] Figure 5 is the schematic diagram of the partial cross-sectional structure of the top cover in an embodiment of the present utility model;

[0027] Figure 6 is the schematic diagram of the exploded structure in an embodiment of the present utility model;

[0028] Figure 7 is the schematic diagram of the installation positions of the first placement groove and the second placement groove in an embodiment of the present utility model;

[0029] Figure 8 is Figure 3Schematic structural diagram of the enlarged part A.

[0030] In the figure: 1. Kit; 11. Bottom box; 12. Top cover; 121. Perforation; 13. Alignment rod; 131. Support frame; 2. Test tube rack; 21. Main frame; 211. First placement groove; 212. Second placement groove; 22. Side plate; 221. Moving groove; 222. Positioning hole; 3. Support unit; 31. Support plate; 32. First elastic member; 4. Fixing unit; 41. Support rod; 42. Pulling plate; 43. Fixing plate; 44. Second elastic member; 5. Test tube. Specific implementation mode

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Please refer to Figures 1-8 , the present invention provides a technical solution: a protein quantitative detection kit, including a kit 1, a test tube rack 2 arranged inside the kit 1, and test tubes 5 placed in the test tube rack 2. The kit 1 includes a bottom box 11 and a top cover 12 covered on the bottom box 11. An alignment rod 13 for supporting the test tube rack 2 is fixedly arranged inside the bottom box 11, and further includes:

[0033] A support unit 3, arranged at the lower end of the top cover 12, for extruding the test tubes 5 placed at the upper end of the test tube rack 2;

[0034] A fixing unit 4, penetrating through both sides of the top cover 12, for fixing the top cover 12 and the alignment rod 13.

[0035] It should be noted that during operation, all components of the reagent kit 1 are restored to room temperature, and then it is checked whether there is precipitation in the protein labeling buffer. If there is precipitation, the reagent can be incubated in a 37°C water bath and gently mixed until the precipitate is completely dissolved. The test tube rack 2 is placed on the alignment rod 13 for fixation, and then multiple test tubes 5 are respectively placed into the test tube rack 2. The prepared working solution is dropped into the test tube 5 one by one. After the detection solution is completely dropped, the fixing unit 4 is pulled outward, the top cover 12 is covered on the bottom box 11, and then the support unit 3 squeezes the test tubes 5 placed on the test tube rack 2. Then the fixing unit 4 is reset so that the fixing unit 4 is clamped and fixed with the alignment rod 13, thereby fixing the top cover 12 and the bottom box 11, so that the support unit 3 fixes the test tube 5 on the test tube rack 2 to complete the fixing work of the test tube 5. Then the reagents inside the test tube 5 are incubated in the dark. Then, according to the operation instructions of the fluorometer, the detection program is selected to measure the fluorescence signal value. By setting this device, the top of the test tube 5 can be supported, and the squeezing force of the support unit 3 on the test tube 5 is small. The friction force between the support unit 3 and the test tube 5 can prevent the test tube 5 from sliding on the test tube rack 2, so that the test tube 5 can be conveniently fixed and the test tube 5 is not easily deformed, which can improve the detection efficiency of protein quantitative detection.

[0036] In one embodiment, the support unit 3 includes a first elastic member 32 fixedly spaced at the lower end of the top cover 12 and a support plate 31 fixed at the lower end of the first elastic member 32. The support plate 31 is adjustably arranged above the test tube rack 2 and is used to support and fix the test tubes 5 placed on the test tube rack 2. The first elastic member 32 is made of a spring or an elastic pad.

[0037] With such a design, when the top cover 12 and the bottom box 11 are fixed, the support plate 31 first abuts against the test tubes 5 on the test tube rack 2. Then, when the top cover 12 is fixed to the alignment rod 13 through the fixing unit 4, the support plate 31 squeezes the first elastic member 32, and the first elastic member 32 can elastically support the support plate 31, which can make the support plate 31 support the test tube 5 more stably.

[0038] In one embodiment, the fixing unit 4 includes a support rod 41 passing through the top cover 12, a pull plate 42 fixed on the outer side of the support rod 41 located outside the top cover 12, and a fixing plate 43 fixed on the inner side of the support rod 41 located inside the top cover 12 for fixing the support rod 41 and the alignment rod 13. A through hole 121 for the support rod 41 to move is provided at the position of the top cover 12 corresponding to the support rod 41.

[0039] With such a design, when the pull plate 42 is pulled towards the side away from the top cover 12, the support rod 41 drives the fixed plate 43 to move towards the side away from the test tube rack 2. The fixed plate 43 fits against the inside of the top cover 12, and then the top cover 12 is capped on the upper end of the bottom box 11. Then, the pull plate 42 is pushed towards the side close to the test tube rack 2, and the fixed plate 43 is snapped onto the lower end of the guiding rod 13 to fix the test tube rack 2 and the bottom box 11.

[0040] In one embodiment, a second elastic member 44 is sleeved between the top cover 12 and the fixed plate 43 on the support rod 41.

[0041] With such a design, when the fixed plate 43 moves outward, the second elastic member 44 is squeezed. Then, after the top cover 12 is fixed on the upper end of the bottom box 11, the pull plate 42 is released. The second elastic member 44 can drive the fixed plate 43 to perform a reset movement and snap onto the guiding rod 13. Moreover, the second elastic member 44 can elastically support the fixed plate 43, making the connection between the fixed plate 43 and the guiding rod 13 more stable.

[0042] In one embodiment, a support frame 131 for supporting the test tube rack 2 is fixedly arranged on the guiding rod 13, and the fixed plate 43 is adjustably arranged below the support frame 131;

[0043] The top end of the fixed plate 43 abuts against the lower end of the support frame 131.

[0044] With such a design, the fixed plate 43 is moved and placed at the lower end of the support frame 131. The support frame 131 can support the test tube rack 2 and can also limit the fixed plate 43, so that the top cover 12 and the bottom box 11 are fixed.

[0045] In one embodiment, the test tube rack 2 includes a main frame 21 for placing test tubes 5 and side plates 22 fixed on both sides of the main frame 21. The side plates 22 are sleeved on the guiding rod 13, and positioning holes 222 for placing the guiding rod 13 are formed at the positions of the side plates 22 corresponding to the guiding rod 13;

[0046] Moving grooves 221 for the movement of the fixed plate 43 are formed at the positions of the side plates 22 corresponding to the fixed plate 43;

[0047] First placement grooves 211 for placing test tubes 5 are spaced apart at the upper end of the main frame 21;

[0048] Second placement grooves 212 for placing test tubes 5 are spaced apart at the lower end of the main frame 21;

[0049] The first placement grooves 211 and the second placement grooves 212 have different diameter sizes and are staggered.

[0050] With such a design, the first placement groove 211 and the second placement groove 212 can place test tubes 5 of different sizes, enabling the test tubes 5 to fit more closely to the inner walls of the placement grooves, so that the test tubes 5 can be placed more stably inside the placement grooves. The top of the test tube 5 protrudes and abuts and fixes against the support plate 31 at the lower end of the top cover 12. The alignment rod 13 can fix the installation position of the side plate 22, facilitating the quick installation and fixation of the test tube rack 2, thus facilitating the next step of placing the test tube 5 in the placement groove opened on the test tube rack 2 and improving the test efficiency of protein quantitative detection.

[0051] In addition, if there are descriptions involving "first", "second", etc. in the embodiments, such descriptions of "first", "second", etc. are for descriptive purposes only and should not be construed as indicating or implying their relative importance to the specification or implicitly indicating the quantity of the indicated technical features. Thus, features defined with "first", "second" may explicitly or implicitly include at least one such feature.

Claims

1. A protein quantitative detection kit, comprising a kit (1), a test tube rack (2) arranged inside the kit (1), and test tubes (5) placed in the test tube rack (2). The kit (1) includes a bottom box (11) and a top cover (12) lid-connected to the bottom box (11). A guiding rod (13) for supporting the test tube rack (2) is fixedly arranged inside the bottom box (11), and it is characterized in that, Further comprising: A support unit (3) is provided at the lower end of the top cover (12) for squeezing the test tube (5) placed at the upper end of the test tube rack (2); A fixing unit (4) is inserted through both sides of the top cover (12) for fixing the top cover (12) and the guiding rod (13).

2. The protein quantitative detection kit according to claim 1, wherein: The support unit (3) includes a first elastic member (32) fixedly spaced at the lower end of the top cover (12) and a support plate (31) fixed at the lower end of the first elastic member (32). The support plate (31) is adjustably disposed above the test tube rack (2) for supporting and fixing the test tube (5) placed on the test tube rack (2).

3. A protein quantitative detection kit according to claim 1, wherein: The fixing unit (4) includes a support rod (41) inserted through the top cover (12), a pulling plate (42) fixed on the outer side of the support rod (41) outside the top cover (12), and a fixing plate (43) fixed on the inner side of the support rod (41) inside the top cover (12) for fixing the support rod (41) and the guiding rod (13). A through hole (121) for the support rod (41) to move is provided at the corresponding position of the top cover (12) with respect to the support rod (41).

4. A protein quantitative detection kit according to claim 3, characterized in that: A second elastic member (44) is sleeved between the top cover (12) and the fixing plate (43) of the support rod (41).

5. A protein quantitative detection kit according to claim 3, characterized in that: A support frame (131) for supporting the test tube rack (2) is fixedly provided on the guiding rod (13), and the fixing plate (43) is adjustably disposed below the support frame (131); The top end of the fixing plate (43) abuts against the lower end of the support frame (131).

6. A protein quantitative detection kit according to claim 5, characterized in that: The test tube rack (2) includes a main frame (21) for placing the test tube (5) and side plates (22) fixed on both sides of the main frame (21). The side plates (22) are inserted through the guiding rod (13), and positioning holes (222) for placing the guiding rod (13) are provided at the corresponding positions of the side plates (22) with respect to the guiding rod (13); A moving groove (221) for the fixing plate (43) to move is provided at the corresponding position of the side plate (22) with respect to the fixing plate (43); First placement grooves (211) for placing the test tube (5) are spaced apart at the upper end of the main frame (21); Second placement grooves (212) for placing the test tube (5) are spaced apart at the lower end of the main frame (21); The first placement grooves (211) and the second placement grooves (212) have different diameter sizes and are arranged staggered.

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