Device for batch digestion operation of cell culture bottle

Abstract

The utility model relates to the technical field of cell culture flask digestion equipment, in particular to a device for batch digestion operation of cell culture flasks, which comprises an L-shaped support, a tray rack, a telescopic cylinder and a plurality of telescopic buckles, the tray rack comprises a plurality of layers of fixedly connected trays, the tray rack is rotatably connected to the L-shaped support, and the telescopic cylinder is arranged on the L-shaped support. An inclined fixing plate is arranged on the L-shaped support, the telescopic air cylinder is installed on the inclined fixing plate, an output shaft of the telescopic air cylinder is hinged to the tray frame, the cell culture bottle is fixed to the tray frame through a telescopic buckle, and the tray frame is driven to rotate through telescopic movement of the telescopic air cylinder. Therefore, the effect that cleaning liquid or digestive juice in the bottle is in full contact with cells attached to the wall is achieved, digestion operation of a plurality of cell culture bottles is completed in batches at a time, convenience and rapidness are achieved, and the problem that the efficiency is low when batch operation is needed due to an existing single culture bottle digestion mode is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture flask digestion equipment, in particular to a device for batch digestion operation of cell culture flasks. Background Technique

[0002] In production practice, at present, the vast majority of animal cells cultured must adhere to the surface of a support to grow, which is also called adherent cells. Therefore, a rectangular culture flask that can provide a cell attachment surface is one of the most common cell culture containers. During cell passage, a digestion reagent (such as 0.25% trypsin) is needed to dissociate the cells from the surface of the culture container and separate them into a suspension of single cells, and then pass them to a container containing fresh culture medium. This step is also called cell digestion. Cell digestion involves steps such as cell washing, digestion, and termination of digestion. After adding the reagent in each step, it is necessary to gently shake the flask to make the reagent fully contact with the cells, a certain reaction time, and the operation of removing the waste liquid after the reaction. Therefore, cell digestion is a laborious and time-consuming process; the existing single culture flask digestion method leads to low efficiency when batch operation is required, and due to the lack of a special culture flask bracket for digestion, it is easy to cause confusion in the operation steps during digestion, thereby increasing the risk of cross-contamination, which hinders the high efficiency and quality assurance of cell passage. Content of the Utility Model

[0003] The utility model aims to provide a device for batch digestion operation of cell culture flasks to solve the problem of low efficiency when batch operation is required by the existing single culture flask digestion method; and a culture flask bracket is provided, which can effectively avoid confusion in operation steps and reduce the risk of cross-contamination.

[0004] To achieve the above purpose, the basic scheme of the utility model is as follows: A device for batch digestion operation of cell culture flasks includes an L-shaped bracket, a tray rack, a telescopic cylinder, and several telescopic buckles. The tray rack includes several layers of trays fixedly connected. The tray rack is rotatably connected to the L-shaped bracket. An inclined fixing plate is provided on the L-shaped bracket. The telescopic cylinder is installed on the inclined fixing plate. The output shaft of the telescopic cylinder is hinged to the tray rack. Several telescopic buckles are installed on the trays to fix cell culture flasks of different specifications.

[0005] Further, it also includes several inclined waste liquid drainage grooves, a waste liquid flow channel, and a waste liquid cup. Several inclined waste liquid drainage grooves are respectively fixed at the front ends of several trays. The waste liquid flow channel is fixed on the side of the tray rack, and the waste liquid flow channel is communicated with several waste liquid drainage grooves. A waste liquid outlet is provided at the bottom of the waste liquid flow channel. The waste liquid cup is placed directly below the waste liquid outlet.

[0006] Further, there are three layers of trays on the tray rack, and 5 groups of telescopic buckles are provided on each tray.

[0007] Furthermore, a rotating shaft is fixed to the side of the top of the tray rack, and the rotating shaft is rotatably connected to the L-shaped bracket.

[0008] Furthermore, there are two tilt fixing plates and two telescopic cylinders. The two tilt fixing plates are respectively installed on both sides of the L-shaped bracket, and the two telescopic cylinders are respectively installed on the two tilt fixing plates.

[0009] Beneficial effects of this solution: (1) Cell culture bottles of different specifications are fixed on the tray rack through telescopic buckles. The telescopic cylinder drives the tray rack to rotate through telescopic movement, so as to achieve the effect that the cleaning liquid or digestive juice in the bottle is in full contact with the adherent cells, and batch complete the digestion operation of multiple cell culture bottles, which is convenient and fast.

[0010] (2) When the telescopic cylinder is pressed down to the bottom, the tray rack tilts downward to the maximum extent, and the liquid that needs to be discarded in the culture bottle can be poured out. The poured liquid flows through the inclined waste liquid drainage groove and the waste liquid flow channel, and finally flows out through the waste liquid outlet into the waste liquid cup to complete the discharge and collection of the waste liquid. Description of the Drawings

[0011] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention. Detailed Embodiments

[0012] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0013] The reference numerals in the drawings of the specification include: L-shaped bracket 1, tray rack 2, telescopic cylinder 3, telescopic buckle 4, inclined waste liquid drainage groove 5, waste liquid flow channel 6, waste liquid cup 7, tilt fixing plate 8.

[0014] Embodiment

[0015] Basically as shown in the attached Figure 1As shown: A device for batch digestion operation of cell culture flasks, comprising an L-shaped bracket 1, a tray rack 2, two telescopic cylinders 3, fifteen groups of fasteners 4, three inclined waste liquid drainage grooves 5, a waste liquid flow channel 6 and a waste liquid cup 7. The tray rack 2 includes three layers of fixedly connected trays. A rotating shaft is fixedly installed on the side of the top of the tray rack 2, and the rotating shaft is rotatably connected to the L-shaped bracket 1. Two inclined fixing plates 8 are provided on both sides of the L-shaped bracket 1. Two telescopic cylinders 3 are respectively installed on the two inclined fixing plates 8. The output shaft of the telescopic cylinder 3 is hinged to the tray rack 2. 5 groups of fasteners 4 are installed on each layer of the tray for fixing the culture flasks. Three inclined waste liquid drainage grooves 5 are respectively fixed at the front ends of the three trays. The waste liquid flow channel 6 is fixed on the side of the tray rack 2, and the waste liquid flow channel 6 communicates with the three inclined waste liquid drainage grooves 5. A waste liquid outlet is provided at the bottom of the waste liquid flow channel 6. The waste liquid cup 7 is placed directly below the waste liquid outlet.

[0016] The specific implementation process is as follows: Cell culture flasks of different specifications are fixed on the tray rack 2 through the telescopic fasteners 4. The telescopic movement of the telescopic cylinder 3 drives the tray rack 2 to rotate, so as to achieve the effect that the cleaning liquid or digestive juice in the flask is in full contact with the adherent cells, and the digestion operation of multiple cell culture flasks is completed batchwise. When the telescopic cylinder 3 is pressed down to the bottom, the tray rack 2 tilts downward to the maximum extent, and the liquid that needs to be discarded in the culture flask can be poured out. The poured liquid passes through the inclined waste liquid drainage groove 5 and the waste liquid flow channel 6, and finally flows out through the waste liquid outlet to the waste liquid cup 7, completing the discharge and collection of the waste liquid.

[0017] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0018] The above are only embodiments of the present utility model. Common general knowledge such as specific structures and characteristics known in the art are not described in detail herein. Those of ordinary skill in the art know all the common general knowledge in the technical field to which the utility model pertains before the filing date or the priority date, can learn all the prior art in this field, and have the ability to apply conventional experimental means before this date. Those of ordinary skill in the art can, under the inspiration given by this application, perfect and implement this solution in combination with their own abilities. Some typical well-known structures or well-known methods should not become obstacles for those of ordinary skill in the art to implement this application. It should be noted that for those skilled in the art, without departing from the structure of the present utility model, several deformations and improvements can still be made, which should also be regarded as the protection scope of the present utility model, and these will not affect the implementation effect of the present utility model and the practicality of the patent. The protection scope claimed in this application shall be subject to the content of its claims, and the specific implementation manners and the like recorded in the specification can be used to interpret the content of the claims.

Claims

1. An apparatus for batch digestion operation of cell culture flasks, characterized in that: It includes an L-shaped bracket, a tray rack, a telescopic cylinder and a number of telescopic fasteners. The tray rack includes a number of trays fixedly connected. The tray rack is rotatably connected to the L-shaped bracket. An inclined fixing plate is provided on the L-shaped bracket. The telescopic cylinder is installed on the inclined fixing plate. The output shaft of the telescopic cylinder is hinged to the tray rack. A number of the telescopic fasteners are installed on the trays for fixing cell culture bottles of different specifications.

2. The device for batch digestion operation of cell culture flasks according to claim 1, wherein: It further includes a number of inclined waste liquid drainage grooves, a waste liquid flow channel and a waste liquid cup. A number of inclined waste liquid drainage grooves are respectively fixed at the front ends of a number of trays. The waste liquid flow channel is fixed on the side of the tray rack, and the waste liquid flow channel is communicated with a number of waste liquid drainage grooves. A waste liquid outlet is provided at the bottom of the waste liquid flow channel. The waste liquid cup is placed directly below the waste liquid outlet.

3. The device for batch digestion operation of cell culture flasks according to claim 2, wherein: There are three layers of trays on the tray rack, and 5 groups of telescopic fasteners are provided on each layer of the tray.

4. A device for batch digestion operation of cell culture flasks according to claim 3, characterized in that: A rotating shaft is fixed to the side of the top of the tray rack, and the rotating shaft is rotatably connected to the L-shaped bracket.

5. A device for batch digestion operation of cell culture flasks according to claim 1, characterized in that: There are two inclined fixing plates and two telescopic cylinders. The two inclined fixing plates are respectively installed on both sides of the L-shaped bracket, and the two telescopic cylinders are respectively installed on the two inclined fixing plates.

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