Quantitative germ inoculation device adopting acupuncture method

Abstract

The utility model discloses a device for quantitatively inoculating germs by using an acupuncture method. The device comprises an inoculation injection needle, an operating handle, a foot switch, a conveying hose, a quantitative conveying pump and a strain container, the inoculation injection needle is detachably connected to one end of the operating handle, the other end of the operating handle is connected with one end of the quantitative delivery pump through one delivery hose, and the other end of the quantitative delivery pump is connected with the strain container through the other delivery hose; and the foot switch is electrically connected with the quantitative delivery pump and is used for controlling the quantitative delivery pump to start. According to the utility model, the operation process of bacteria or other pathogenic bacteria inoculation experiments performed by researchers can be simplified, the inoculation amount can be quantitatively controlled, and the inoculation efficiency and the experiment accuracy can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental instruments, in particular to a device for quantitatively inoculating pathogenic bacteria by acupuncture method. Background Art

[0002] In the study of plant-microbe interaction, pathogenic bacteria, as an important type of plant pathogenic microorganism, cause major diseases in many crops and have serious adverse effects. To study the virulence expression and regulation of plant pathogenic bacteria, it is necessary to infect plants with pathogenic bacteria. Acupuncture inoculation is a commonly used method for inoculating plant pathogenic bacteria. Taking the inoculation of Xanthomonas citri subsp. citri as an example, during inoculation, an inoculation needle is dipped into the bacterial suspension, and then the sample to be inoculated (leaves, branches, fruits) of the plant is punctured to successfully inoculate. However, the inoculation method of dipping the bacteria attached to the inoculation needle onto the acupuncture point cannot ensure consistent inoculation amount. If quantitative inoculation of pathogenic bacteria is required in an experiment, an inoculation needle without bacteria can be used to puncture inoculation points on the sample to be inoculated, and then a pipette can be used to quantitatively transfer the bacterial suspension to each inoculation point one by one to successfully inoculate. When the inoculated area of the sample to be inoculated is on the same plane, a certain number of inoculation needles can be fixed on the same carrier according to the size of the sample and the interval distance between the inoculation points, and then a pipette is used to transfer the bacterial suspension to each inoculation point to improve the efficiency of the inoculation work.

[0003] However, in most experiments, for the situation where the sample size is large and each sample has multiple inoculation points, or the sample to be inoculated is plant tissue such as fruits and branches with an irregular curved surface for the inoculation point area, the scheme of fixing multiple inoculation needles on the same carrier is not applicable, but a single inoculation needle is used for inoculation operation. When quantitative inoculation of the bacterial suspension is required in the above situations, the following technical problems will be brought to the inoculation work: 1. Using an inoculation needle in combination with a pipette for inoculation has the problems of low inoculation efficiency and slow speed. This process requires frequent replacement of operation tools to perform operations such as acupuncture and pipette inoculation; 2. Using a combination of multiple inoculation needles in combination with a pipette for inoculation has the problem that due to the inoculation points not being on the same plane, the puncture depth is not uniform, resulting in a large error in the inoculation amount. Therefore, the current technical problem is that there is no inoculation tool that can accurately control the inoculation amount while having a relatively fast inoculation speed. Therefore, it is necessary to design a device for quantitatively inoculating pathogenic bacteria by acupuncture method. Summary of the Invention

[0004] The utility model provides a device for quantitatively inoculating pathogenic bacteria by acupuncture method, which can quantitatively control the inoculation amount of pathogenic bacteria, simplify the inoculation steps, so as to improve the inoculation efficiency and the accuracy of the experiment at the same time.

[0005] To achieve the above object, the technical solution adopted by the utility model is as follows:

[0006] A quantitative inoculation device for acupuncture method, comprising an inoculation injection needle, an operating handle, a foot switch, a delivery hose, a quantitative delivery pump and a strain container; the inoculation injection needle is detachably connected to one end of the operating handle, and the other end of the operating handle is connected to one end of the quantitative delivery pump through a delivery hose, and the other end of the quantitative delivery pump is connected to the strain container through another delivery hose; the foot switch is electrically connected to the quantitative delivery pump for controlling the start of the quantitative delivery pump.

[0007] Further, the operating handle includes an injection needle connector and a holding handle; one end of the inoculation injection needle is threadedly connected to the front end of the injection needle connector; the holding handle is provided as a hollow circular tube structure, and one end of the delivery hose passes through the holding handle and is connected to the rear end of the injection needle connector; the holding handle is detachably connected to the outer peripheral side of the rear end of the injection needle connector.

[0008] Further, a connecting pipe extends axially outwards from the rear end of the injection needle connector, and the inner wall of the delivery hose is in interference fit with the outer peripheral side of the connecting pipe; an annular connecting portion is provided on the outer wall of the connecting pipe, and the annular connecting portion is coaxially arranged with the connecting pipe; a plurality of cracks are equally angled on the annular connecting portion, so that the annular connecting portion is divided into several claw-like parts, a taper thread is provided on the outer peripheral side of the annular connecting portion, and a taper thread matching the outer peripheral side of the annular connecting portion is provided at one end of the holding handle, and the holding handle is connected to the annular connecting portion through the taper thread, so that several claw-like parts contract inwards, and cooperate with the connecting pipe to press the delivery hose tightly.

[0009] Further, an annular rib is formed by extending outwards at the connecting end of the injection needle connector and the holding handle.

[0010] Further, the injection needle connector is made of pp material.

[0011] Further, the whole holding handle is made of PP material, and the outer wall is wrapped with silica gel material.

[0012] Further, the quantitative delivery pump adopts a peristaltic pump or a micro pump.

[0013] The beneficial effects of the present utility model are as follows:

[0014] 1) The present utility model uses an inoculation injection needle to puncture or punch holes at a selected inoculation position, starts the delivery pump through a foot switch, extracts the bacterial suspension from the strain container through a quantitative delivery pump and quantitatively flows out from the inoculation injection needle, realizes the functions of micro inoculation and quantitative control, achieves the purpose of immediately delivering the bacterial suspension to the inoculation point every time an inoculation point is stabbed, simplifies the operation of bacterial inoculation, improves the inoculation efficiency, and also improves the accuracy of the experiment.

[0015] 2) The holding handle and the annular connecting part are connected by taper threads, causing each claw to contract inward, cooperating with the connecting pipe to press the delivery hose tightly, preventing the delivery hose from loosening, and at the same time improving the sealing effect; one end of the delivery hose passes through the holding handle and is connected to the rear end of the injection needle connector. Such a design enables the bacterial suspension to be directly sent to the injection needle connector without contaminating the holding handle, reducing the risk of contamination.

[0016] 3) The function of the holding handle is to provide a main body for the user to apply force, and it can be held and operated in the way of holding a pen.

[0017] 4) One end of the injection needle connector connected to the holding handle extends outward to form an annular flange, which can prevent the hand from slipping during holding. Description of the Drawings

[0018] The following further elaborates on the specific embodiments of the present invention in conjunction with the drawings, where:

[0019] Figure 1 is a schematic structural diagram of the present invention;

[0020] Figure 2 is a perspective view of the inoculation injection needle, operation handle and delivery hose in the present invention;

[0021] Figure 3 is an exploded view of the inoculation injection needle, operation handle and delivery hose in the present invention;

[0022] Figure 4 is a perspective view of the injection needle connector in the present invention;

[0023] Reference Signs in the Drawings:

[0024] 1 - Inoculation injection needle, 2 - Operation handle, 3 - Foot switch, 4 - Delivery hose, 5 - Quantitative delivery pump, 6 - Bacterial strain container, 21 - Injection needle connector, 22 - Holding handle, 211 - Connecting pipe, 212 - Annular connecting part, 213 - Annular flange. Detailed Embodiments

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0026] It should be noted that when a component is referred to as "fixed to" another component, it can be directly on the other component or there may be an intermediate component. When a component is considered to be "connected to" another component, it can be directly connected to the other component or there may be an intermediate component at the same time. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be an intermediate component at the same time. When a part is referred to as "disposed in the middle", it is not only disposed at the exact middle position, but as long as it is not disposed at the two ends, it falls within the range defined by the middle. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this utility model belongs. The terms used herein in the specification of this utility model are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0028] Refer to Figures 1 to 4 As shown, a quantitative inoculation device for pathogenic bacteria by acupuncture includes an inoculation injection needle 1, an operation handle 2, a foot switch 3, a delivery hose 4, a quantitative delivery pump 5 and a bacterial strain container 6.

[0029] Among them, one end of the inoculation injection needle 1 is provided with a sharp inoculation needle, and the inoculation injection needle can be set with different specifications to meet the requirements of different sizes of inoculation points during experiments; the other end of the inoculation injection needle 1 is provided with an external thread, and the inoculation injection needle 1 is threadedly connected to one end of the operation handle 2 to achieve a detachable function for convenient replacement.

[0030] The operating handle 2 includes an injection needle connector 21 and a holding handle 22; the injection needle connector 21 is made of PP material, and one end of the inoculation injection needle 1 is threadedly connected to the front end of the injection needle connector 21; the holding handle 22 is designed as a hollow circular tube structure, the whole of the holding handle 22 is made of PP material, and the outer wall is wrapped with silica gel material. One end of the delivery hose 4 passes through the holding handle 22 and is connected to the rear end of the injection needle connector 21, and one end of the delivery hose 4 is connected to one end of the metering delivery pump 5. Such a design can enable the bacterial liquid to be directly sent to the injection needle connector 21 without contaminating the holding handle 22, reducing the risk of contamination. The holding handle 22 is detachably connected to the outer peripheral side of the rear end of the injection needle connector 21. The function of the holding handle 22 is to provide a main body for the user to apply force, and the operation can be held in the way of holding a pen. The other end of the metering delivery pump 5 is connected to the bacterial strain container 6 through another delivery hose 4. The metering delivery pump 5 extracts and quantitatively delivers the bacterial suspension from the bacterial strain container 6 to the inoculation injection needle 1. The metering delivery pump 5 adopts a peristaltic pump or a micro pump, and can perform micro delivery in microliters. Both can achieve single or continuous multiple quantitative and precise delivery and the delivery volume is adjustable, which can achieve the purpose of quantitative inoculation and micro inoculation; the foot switch 3 is electrically connected to the metering delivery pump 5 and is used to control the start of the metering delivery pump 5. When holding the operation handle 2 for inoculation operation, using the foot switch 3 can make the hand focus on maintaining the position without performing other force-applying actions, and keep the inoculation position accurate as much as possible.

[0031] In the experiment of the present utility model, leaves, branches or fruits, etc. can be used as plant materials for inoculating pathogenic bacteria. According to the shape, size and inoculation requirements of the plant tissue materials, the inoculation position, inoculation quantity and concentration of the bacterial suspension are determined. The bacterial suspension is filled into the bacterial strain container 6. The tip of the inoculation injection needle 1 is used to puncture or make an inoculation point on the plant sample. The metering delivery pump 5 is started by the foot switch 3. The metering delivery pump 5 extracts and quantitatively delivers the bacterial suspension from the bacterial strain container 6 to the inoculation injection needle 1, realizing the functions of micro inoculation and quantitative control, and can also achieve the purpose of immediately delivering the bacterial suspension to the inoculation point as soon as each inoculation point is made, simplifying the pathogen inoculation operation, improving the experimental efficiency, and at the same time improving the accuracy of the experiment.

[0032] Since the operating handle 2 needs to be held and moved by the operator, a pulling force will be formed on the connection position between the conveying hose 4 and the injection needle connector 21, which is likely to become loose. To solve this problem, in the present utility model, a connecting pipe 211 is extended outward along the central axis at the rear end of the injection needle connector 21, and the inner wall of the conveying hose 4 is in interference fit with the outer peripheral side of the connecting pipe 211; an annular connecting portion 212 is provided on the outer wall of the connecting pipe 211, the inner diameter of the annular connecting portion 212 is larger than the outer diameter of the connecting pipe 211, and the annular connecting portion 212 is coaxially arranged with the connecting pipe 211; a plurality of cracks are equally angularly provided on the annular connecting portion 212, so that the annular connecting portion 212 is divided into several claw-like parts, a taper thread is provided on the outer peripheral side of the annular connecting portion 212, and a taper thread matching the outer peripheral side of the annular connecting portion 212 is provided at one end of the holding handle 22. The holding handle 22 is connected to the annular connecting portion 212 through the taper thread, so that each claw-like part contracts inward, and cooperates with the connecting pipe 211 to press the conveying hose 4 tightly, preventing the conveying hose 4 from becoming loose, and at the same time improving the sealing effect.

[0033] One end of the injection needle connector 21 connected to the holding handle 22 extends outward to form an annular edge 213, which can prevent the hand from slipping when holding.

[0034] The usage method of the present utility model:

[0035] 1) Prepare experimental materials: Before inoculation, prepare the plant materials to be inoculated (such as leaves, branches, fruits), the bacterial suspension (poured into the bacterial species container), and connect the pipelines.

[0036] 2) Instrument setting: Set the single delivery volume of the metering pump 5 according to the concentration and inoculation amount of the bacterial suspension. The delivery method and delivery volume can be determined according to the experimental plan.

[0037] 3) Inoculation: The operator holds the operating handle 2 by hand, and uses the tip of the inoculation needle 1 to puncture or make wounds on the plant materials as inoculation sites respectively. The number and positions of the inoculation sites are determined according to the experimental requirements. After each needle punctures an inoculation point, keep the position, and at the same time control the foot switch 3 to start the metering pump 5 to quantitatively deliver the bacterial suspension to the inoculation point; perform inoculation in sequence according to the inoculation point positions required by the experiment to quickly complete the inoculation operation.

[0038] The above embodiments are only used to illustrate the technical solutions of the present utility model and are not intended to limit it. Any modification or equivalent replacement that does not depart from the spirit and scope of the present utility model shall be covered within the scope of the technical solutions of the present utility model.

Claims

1. A quantitative inoculation device for pathogenic bacteria by acupuncture method, characterized in that, It includes an inoculation injection needle, an operation handle, a foot switch, a delivery hose, a metering delivery pump, and a strain container; the inoculation injection needle is detachably connected to one end of the operation handle, the other end of the operation handle is connected to one end of the metering delivery pump through one of the delivery hoses, and the other end of the metering delivery pump is connected to the strain container through another delivery hose; the foot switch is electrically connected to the metering delivery pump and is used to control the start of the metering delivery pump.

2. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 1, characterized in that, The operation handle includes an injection needle connector and a gripping handle; one end of the inoculation injection needle is threadedly connected to the front end of the injection needle connector; the gripping handle is provided as a hollow circular tube structure, one end of the delivery hose passes through the gripping handle and is connected to the rear end of the injection needle connector; the gripping handle is detachably connected to the outer peripheral side of the rear end of the injection needle connector.

3. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 2, characterized in that, A connecting tube extends axially outward from the rear end of the injection needle connector, and the inner wall of the delivery hose is in interference fit with the outer peripheral side of the connecting tube; an annular connecting portion is provided on the outer wall of the connecting tube, and the annular connecting portion is coaxially arranged with the connecting tube; the annular connecting portion is provided with a number of cracks at equal angles, so that the annular connecting portion is divided into a number of claw-shaped parts, a tapered thread is provided on the outer peripheral side of the annular connecting portion, and a tapered thread matching the outer peripheral side of the annular connecting portion is provided at one end of the gripping handle, and the gripping handle is connected to the annular connecting portion through the tapered thread, so that the number of the claw-shaped parts contracts inward, and cooperates with the connecting tube to press the delivery hose tightly.

4. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 3, characterized in that, An annular flange extends outward from the end of the injection needle connector where it is connected to the gripping handle.

5. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 4, characterized in that, The injection needle connector is made of PP material.

6. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 3, characterized in that, The gripping handle is integrally made of PP material, and the outer wall is wrapped with silica gel material.

7. The quantitative inoculation device for pathogenic bacteria by acupuncture method according to claim 1, characterized in that, The metering delivery pump uses a peristaltic pump or a micro pump.

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