Multi-mode observation system for micro-fluidic chip

Abstract

The utility model relates to a multi-mode observation system for a micro-fluidic chip. The multi-mode observation system comprises a box body; the global observation module is mounted in the box body; the optical quantitative detection module is movably installed in the box body through a first moving assembly, and the first moving assembly drives the optical quantitative detection module to move in the first horizontal direction; the microscopic observation module is movably installed in the box body through a second moving assembly, and the second moving assembly drives the microscopic observation module to move in the first horizontal direction; the chip bearing seat is installed in the box body through a third moving assembly, and the third moving assembly drives the chip bearing seat to move in the second horizontal direction; when the chip bearing seat moves, the three parts are switched back and forth. According to the utility model, various observations can be realized, experimental errors caused by frequent change of samples among a plurality of devices are avoided, the reliability of results is improved, and the device has the characteristics of portability, quick deployment, quick response and the like.

Description

Technical Field

[0001] The present application relates to the field of microfluidic observation technology, and in particular to a multimodal observation system for a microfluidic chip. Background Art

[0002] Microfluidics refers to the use of microchannels to process or manipulate tiny fluids. It is able to operate fluids at the microscopic level. It has shown great potential in the fields of biomedical research, environmental monitoring, and food safety testing. Microfluidics observation involves many aspects of processing, such as global distribution observation of samples, meticulous observation of local details, and accurate quantitative readout of detection signals. However, in the prior art, these processing processes usually need to be implemented in different devices, resulting in the need to transfer samples between different devices. The experimental environment before and after the sample transfer is more or less different, and the sample is easily adversely affected during the transfer process, which may lead to experimental errors. Utility Model Content

[0003] Based on this, it is necessary to provide a multimodal observation system for microfluidic chips, and its specific technical solution is as follows.

[0004] A multimodal observation system for a microfluidic chip, characterized by comprising:

[0005] Box;

[0006] Global observation module, installed in the box;

[0007] The optical quantitative detection module is movably installed in the box body through a first moving component, and the first moving component drives the optical quantitative detection module to move along a first horizontal direction;

[0008] The microscopic observation module is movably installed in the box body through a second moving component, and the second moving component drives the microscopic observation module to move along a first horizontal direction; the global observation module, the optical quantitative detection module, and the microscopic observation module are arranged along a second horizontal direction, and the first horizontal direction is perpendicular to the second horizontal direction;

[0009] The chip carrier is installed in the box through a third moving component, and the third moving component drives the chip carrier to move along the second horizontal direction; the chip carrier is located below the global observation module, the optical quantitative detection module, and the microscopic observation module, and the chip carrier switches back and forth between the three when moving.

[0010] Furthermore, it also includes a magnetic control module, which is located below the global observation module and is used to provide a magnetic field to enrich the sample in the microfluidic chip.

[0011] Furthermore, it also includes a temperature control module, which is located below the optical quantitative detection module and is used to adjust the temperature.

[0012] Further, the number of the global observation module, the optical quantitative detection module, and the microscopic observation module is one or more.

[0013] Further, the global observation module includes:

[0014] A first light source module, including a full-wavelength LED, a collimating mirror, a first light-shielding wheel, and a concave lens; the light emitted by the full-wavelength LED sequentially passes through the collimating mirror, the first light-shielding wheel, and the concave lens and then irradiates the microfluidic chip;

[0015] A second light source module, including a first illumination light source, and the first illumination light source irradiates the microfluidic chip;

[0016] An acquisition module, including a first photosensitive coupling component, a lens, and a second light-shielding wheel, and the reflected light of the microfluidic chip is sequentially received by the first photosensitive coupling component after passing through the second light-shielding wheel and the lens.

[0017] Further, the first light-shielding wheel is connected to a first motor, and a plurality of first light-shielding sheets are arranged in a circumferential array on the first light-shielding wheel, and the first light-shielding wheel is driven by the first motor to rotate to switch different first light-shielding sheets to be penetrated by the light emitted by the full-wavelength LED;

[0018] The second light-shielding wheel is connected to a second motor, and a plurality of second light-shielding sheets are arranged in a circumferential array on the second light-shielding wheel, and the second light-shielding wheel is driven by the second motor to rotate to switch different second light-shielding sheets to be penetrated by the reflected light of the microfluidic chip.

[0019] Further, the optical quantitative detection module includes an LED excitation light source, a first filter, a first collimating lens, and a first dichroic mirror; the light generated by the LED excitation light source is emitted to the microfluidic chip through the first filter, the first collimating lens, and the first dichroic mirror; the optical quantitative detection module further includes a second collimating lens, a second filter, and a micro detector; the reflected light of the microfluidic chip is collected by the micro detector after sequentially passing through the first dichroic mirror, the second collimating lens, and the second filter.

[0020] Further, the microscopic observation module includes a second illumination light source, a third collimating lens, a second dichroic mirror, and an objective lens; the light emitted by the second illumination light source irradiates the microfluidic chip after passing through the third collimating lens, the second dichroic mirror, and the objective lens; the microscopic observation module further includes an emission sheet and a second photosensitive coupling component, and the reflected light of the microfluidic chip is collected by the second photosensitive coupling component after passing through the objective lens, the second dichroic mirror, and the emission sheet.

[0021] Beneficial effects: The multi-modal observation system for a microfluidic chip provided by the present utility model integrates a global observation module, an optical quantitative detection module, and a microscopic observation module in a box body, and drives the chip carrier to move by using a third moving component, so that the microfluidic chip can be switched between different modules, realizing multi-faceted observations such as global qualitative observation, quantitative fluorescence or chemical detection, and detail detection. During the detection process, the microfluidic chip always remains in the same environment without the need for transfer, avoiding experimental errors caused by frequent changes of samples among multiple devices and improving the reliability of the results. In addition, by integrating the global observation module, the optical quantitative detection module, and the microscopic observation module in the box body, it is also convenient to carry and can be quickly deployed in various locations or environments, suitable for on-site detection or rapid response in emergency situations. Brief Description of the Drawings

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0023] Figure 1 is the overall schematic diagram of the observation system;

[0024] Figure 2 is the schematic diagram of the principle of the global observation module;

[0025] Figure 3 is the schematic diagram of the principle of the optical quantitative detection module;

[0026] Figure 4 is the schematic diagram of the principle of the microscopic observation module;

[0027] Figure 5 is the schematic diagram of the state of the observation system for global qualitative observation of microfluidic samples;

[0028] Figure 6 is the schematic diagram of the state of the observation system for sample enrichment and global observation;

[0029] Figure 7 is the schematic diagram of the state of the observation system for optical quantitative detection;

[0030] Figure 8 is the schematic diagram of the state of the observation system for local observation and detection.

[0031] Description of the reference numerals:

[0032] 1. Box body; 2. Global observation module; 3. Optical quantitative detection module; 4. Microscopic observation module; 5. Chip carrier; 6. First moving component; 7. Second moving component; 8. Third moving component; 9. Magnetron module; 10. Temperature control module; 11. Microfluidic chip;

[0033] 21. Full-wavelength LED; 22. Collimating mirror; 23. First light-shielding wheel; 24. Concave lens; 25. First illumination light source; 26. First photosensitive coupling component; 27. Lens; 28. Second light-shielding wheel;

[0034] 31. LED excitation light source; 32. First filter; 33. First collimating lens; 34. First dichroic mirror; 35. Second collimating lens; 36. Second filter; 37. Micro detector;

[0035] 41. Second illumination light source; 42. Third collimating lens; 43. Second dichroic mirror; 44. Objective lens; 45. Emission film; 46. Second photosensitive coupling component. Detailed implementation manners

[0036] To make the above objects, features, and advantages of the present application more obvious and understandable, the following will describe the detailed implementation manners of the present application with reference to the accompanying drawings. Many specific details are set forth in the following description to fully understand the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the present application. Therefore, the present application is not limited by the specific embodiments disclosed below.

[0037] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. These are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation to the present application.

[0038] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In the description of the present application, "a plurality of" means at least two, such as two, three, etc., unless otherwise specifically defined.

[0039] In this application, unless otherwise clearly specified or limited, terms such as "installed", "connected", "linked", "fixed", etc. shall be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two components or the interaction relationship between two components, unless otherwise clearly limited. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0040] In this application, unless otherwise clearly specified or limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature being "under", "beneath" and "underneath" the second feature can be that the first feature is directly below or obliquely below the second feature, or merely indicates that the horizontal height of the first feature is less than that of the second feature.

[0041] It should be noted that when an element is referred to as "fixed to" or "disposed on" another element, it can be directly on the other element or there can also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used herein are only for the purpose of illustration and do not represent the only implementation.

[0042] Embodiment

[0043] Refer to Figure 1 As shown, this embodiment provides a multimodal observation system for a microfluidic chip, including a box body 1, a global observation module 2, an optical quantitative detection module 3, a microscopic observation module 4 and a chip carrier 5. It should be noted that for the convenience of showing the specific structure of the observation system, Figure 1 and subsequent Figures 5 - 8 the box body 1 is respectively made transparent.

[0044] Specifically, the global observation module 2 is installed in the box body 1 and is used for globally qualitatively observing the sample on the microfluidic chip 11.

[0045] The optical quantitative detection module 3 is movably installed in the box body 1 through the first moving component 6, and the first moving component 6 drives the optical quantitative detection module 3 to move along the first horizontal direction. The optical quantitative detection module 3 is used for performing quantitative fluorescence detection, quantitative chemiluminescence detection, etc. on the samples on the microfluidic chip 11. By driving the optical quantitative detection module 3 with the first moving component 6, linear scanning of the microfluidic chip 11 can be performed.

[0046] The microscopic observation module 4 is movably installed in the box body 1 through the second moving component 7, and the second moving component 7 drives the microscopic observation module 4 to move along the first horizontal direction. The microscopic observation module 4 is used for observing only the details of the chip on the microfluidic device. By driving the microscopic observation module 4 with the second moving component 7, linear scanning of the microfluidic chip 11 can be performed. The global observation module 2, the optical quantitative detection module 3, and the microscopic observation module 4 are arranged in a row along the second horizontal direction, and the first horizontal direction is perpendicular to the second horizontal direction.

[0047] The chip carrier 5 is installed in the box body 1 through the third moving component 8, and the third moving component 8 drives the chip carrier 5 to move along the second horizontal direction. The chip carrier 5 is located below the global observation module 2, the optical quantitative detection module 3, and the microscopic observation module 4. By driving the chip carrier 5 to move back and forth between the global observation module 2, the optical quantitative detection module 3, and the microscopic observation module 4 with the third moving component 8, and the chip carrier 5 is used for placing the microfluidic chip 11, so as to realize the back-and-forth switching of the microfluidic chip 11 among the three, and realize the switching of different modules for corresponding observations.

[0048] In this embodiment, the first moving component 6, the second moving component 7, and the third moving component 8 can adopt a lead screw structure or a linear motion module and other structures. The specific structures of the lead screw structure or the linear motion module are common knowledge, so they are not described in detail in this embodiment, and other forms of moving structures can also be adopted in other embodiments.

[0049] A multi-modal observation system for a microfluidic chip provided in this embodiment integrates the global observation module 2, the optical quantitative detection module 3, and the microscopic observation module 4 in the box body 1, and drives the chip carrier 5 to move by using the third moving component 8, so that the microfluidic chip 11 can be switched between different modules, realizing multi-faceted observations such as global qualitative observation, quantitative fluorescence or chemical detection, and detail detection. During the detection process, the microfluidic chip 11 is always in the same environment and does not need to be transferred, avoiding experimental errors caused by frequent changes of samples among multiple devices and improving the reliability of the results. In addition, by integrating the global observation module 2, the optical quantitative detection module 3, and the microscopic observation module 4 in the box body 1, it is also convenient to carry and can be quickly deployed in various locations or environments, suitable for on-site detection or rapid response in emergency situations.

[0050] Specifically, it further includes a magneto - control module 9, which is located below the global observation module 2 and is used to provide a magnetic field to enrich the samples in the microfluidic chip 11. The function of the magneto - control module 9 is to provide the magnetic field required for global observation, and the intensity and type of this magnetic field can be changed according to the observation needs. The specific structure of the magneto - control module 9 and how to achieve the change of the magnetic field intensity or the change of the magnetic field type are common knowledge in the art and will not be elaborated herein.

[0051] Specifically, it further includes a temperature - control module 10, which is located below the optical quantitative detection module 3 and is used to adjust the temperature. By adjusting to the corresponding temperature through the temperature - control module 10, various processes such as cell culture, nucleic acid amplification, sample incubation, evaporation and purification of the samples on the microfluidic chip 11 can be respectively realized, and observations in various states can be achieved. The function of the temperature - control module 10 is to provide the corresponding temperature for optical quantitative detection. As for the specific structure of the temperature - control module 10 and how to achieve the temperature regulation of the temperature - control module 10, it belongs to common knowledge and will not be elaborated in this embodiment.

[0052] Specifically, in this embodiment, one or more global observation modules 2, optical quantitative detection modules 3, and microscopic observation modules 4 can be set according to actual needs to meet the corresponding observation requirements.

[0053] Specifically, Figure 2 The schematic diagram of the global observation module 2 and the corresponding components are shown. Refer to Figure 2 As shown, the global observation module 2 includes:

[0054] A first light - source module, including a full - wavelength LED 21, a collimating mirror 22, a first light - shielding wheel 23, and a concave lens 24; the light emitted by the full - wavelength LED 21 passes through the collimating mirror 22, the first light - shielding wheel 23, and the concave lens 24 in sequence and then irradiates onto the microfluidic chip 11;

[0055] A second light - source module, including a first illumination light - source 25, and the first illumination light - source 25 irradiates onto the microfluidic chip 11;

[0056] An acquisition module, including a first photosensitive coupling component 26, a lens 27, and a second light - shielding wheel 28. The reflected light of the microfluidic chip 11 is received by the first photosensitive coupling component 26 after passing through the second light - shielding wheel 28 and the lens 27 in sequence.

[0057] Among them, the first light - shielding wheel 23 is connected to a first motor, and a plurality of first light - shielding sheets are arranged in a circumferential array on the first light - shielding wheel 23. The first light - shielding wheel 23 is driven by the first motor to rotate to switch different first light - shielding sheets through which the light emitted by the full - wavelength LED 21 passes;

[0058] The second light-shielding wheel 28 is connected to the second motor, and a plurality of second light-shielding sheets are arranged in a circumferential array on the second light-shielding wheel 28. The second light-shielding wheel 28 is driven by the second motor to rotate, so that different second light-shielding sheets are penetrated by the reflected light of the microfluidic chip 11. It should be noted that the types of the first light-shielding sheets or the second light-shielding sheets are different, and different types of the first light-shielding sheets or the second light-shielding sheets are switched to adapt to the corresponding observation needs.

[0059] It should be noted that, according to Figure 2 the schematic diagram of the global observation module 2 shown and the above description of the components of the global observation module 2, those skilled in the art should know how the components are arranged to achieve the global qualitative observation of the microfluidic chip 11.

[0060] Specifically, Figure 3 the schematic diagram of the optical quantitative detection module 3 and the corresponding components are shown. Referring to Figure 3 shown, the optical quantitative detection module 3 includes an LED excitation light source 31, a first filter 32, a first collimating lens 33, and a first dichroic mirror 34; the light emitted by the LED excitation light source 31 is emitted to the microfluidic chip 11 through the first filter 32, the first collimating lens 33, and the first dichroic mirror 34; the optical quantitative detection module 3 further includes a second collimating lens 35, a second filter 36, and a micro detector 37; the reflected light of the microfluidic chip 11 is collected by the micro detector 37 after passing through the first dichroic mirror 34, the second collimating lens 35, and the second filter 36 in sequence.

[0061] It should be noted that, according to Figure 3 the schematic diagram of the optical quantitative detection module 3 shown and the above description of the components of the optical quantitative detection module 3, those skilled in the art should know how the components are arranged to achieve the quantitative fluorescence or chemical detection of the microfluidic chip 11.

[0062] Specifically, Figure 4 the schematic diagram of the microscopic observation module 4 and the components are shown. Referring to Figure 4 shown, the microscopic observation module 4 includes a second illumination light source 41, a third collimating lens 42, a second dichroic mirror 43, and an objective lens 44; the light emitted by the second illumination light source 41 is irradiated to the microfluidic chip 11 after passing through the third collimating lens 42, the second dichroic mirror 43, and the objective lens 44; the microscopic observation module 4 further includes an emission sheet 45 and a second photosensitive coupling component 46, and the reflected light of the microfluidic chip 11 is collected by the second photosensitive coupling component 46 after passing through the objective lens 44, the second dichroic mirror 43, and the emission sheet 45.

[0063] It should be noted that, according to Figure 4Based on the schematic diagram of the microscopic observation module 4 shown above and the descriptions of the various components of the microscopic observation module 4, those skilled in the art should be aware of how the components are arranged to achieve detailed observation of the microfluidic chip 11.

[0064] The multimodal observation system for a microfluidic chip provided in this embodiment can perform various forms of observation on the samples on the microfluidic chip 11. According to Figures 5 - 8 Examples of different observation modes are given below.

[0065] Referring to Figure 5 as shown, the chip carrier 5 is moved to below the global observation module 2 by the third moving component 8 to perform global qualitative observation on the microfluidic chip 11.

[0066] Referring to Figure 6 as shown, the chip carrier 5 is moved to below the global observation module 2 by the third moving component 8, and the magnetic control module 9 is activated to apply a magnetic field, so as to enrich the samples on the microfluidic chip 11 and perform global observation in the state of sample enrichment.

[0067] Referring to Figure 7 as shown, the chip carrier 5 is moved to below the optical quantitative detection module 3 by the third moving component 8, and the environmental temperature of the microfluidic chip 11 is controlled by the temperature control module 10 to process the samples, including but not limited to cell culture, nucleic acid amplification, sample incubation, evaporation purification, etc., to achieve quantitative fluorescence or chemical detection. During the detection process, the optical quantitative detection module 3 can also be driven by the first moving component 6 to move along the first horizontal direction, or the chip carrier 5 can be driven by the third moving component 8 to move along the second horizontal direction, so as to realize the detection of different regions of the microfluidic chip 11.

[0068] Referring to Figure 8 as shown, the chip carrier 5 is moved to below the microscopic observation module 4 by the third moving component 8 to perform detailed observation on the microfluidic chip 11, such as observing the cell characteristics in the droplet, etc. During the detailed observation process, the microscopic observation module 4 can also be driven by the second moving component 7 to move along the first horizontal direction, or the chip carrier 5 can be driven by the third moving component 8 to move along the second horizontal direction, so as to realize the detailed observation of different regions of the microfluidic chip 11.

[0069] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity of description, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as the combinations of these technical features do not conflict, they should all be considered as the scope described in this specification.

[0070] The above-described embodiments merely represent several implementation manners of the present application. The description thereof is relatively specific and detailed, but it should not be construed as a limitation on the scope of the patented application. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all fall within the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A multimodal observation system for a microfluidic chip, characterized in that, Comprising: A box body; A global observation module, installed inside the box body; An optical quantitative detection module, movably installed inside the box body through a first moving component, and the first moving component drives the optical quantitative detection module to move along a first horizontal direction; A microscopic observation module, movably installed inside the box body through a second moving component, and the second moving component drives the microscopic observation module to move along the first horizontal direction; the global observation module, the optical quantitative detection module, and the microscopic observation module are arranged in a row along a second horizontal direction, and the first horizontal direction is perpendicular to the second horizontal direction; A chip carrier, installed inside the box body through a third moving component, and the third moving component drives the chip carrier to move along the second horizontal direction; the chip carrier is located below the global observation module, the optical quantitative detection module, and the microscopic observation module, and the chip carrier switches back and forth among the three when moving.

2. The multimodal observation system for a microfluidic chip according to claim 1, characterized in that, It further includes a magnetic control module, located below the global observation module, and is used to provide a magnetic field to enrich the sample in the microfluidic chip.

3. The multimodal observation system for a microfluidic chip according to claim 1, characterized in that, It further includes a temperature control module, located below the optical quantitative detection module, and is used to adjust the temperature.

4. A multimodal observation system for a microfluidic chip according to claim 1, characterized in that, The number of the global observation module, the optical quantitative detection module, and the microscopic observation module is one or more.

5. A multimodal observation system for a microfluidic chip according to claim 1, characterized in that, The global observation module includes: A first light source module, including a full-wavelength LED, a collimating mirror, a first light-shielding wheel, and a concave lens; the light emitted by the full-wavelength LED passes through the collimating mirror, the first light-shielding wheel, and the concave lens in sequence and then irradiates onto the microfluidic chip; A second light source module, including a first illumination light source, and the first illumination light source irradiates onto the microfluidic chip; An acquisition module, including a first photosensitive coupling component, a lens, and a second light-shielding wheel, and the reflected light of the microfluidic chip is received by the first photosensitive coupling component after passing through the second light-shielding wheel and the lens in sequence.

6. The multimodal observation system for a microfluidic chip according to claim 5, wherein: The first light-shielding wheel is connected to a first motor, and a plurality of first light-shielding sheets are arranged in a circumferential array on the first light-shielding wheel, and the first motor drives the first light-shielding wheel to rotate to switch different first light-shielding sheets to be penetrated by the light emitted by the full-wavelength LED; The second light-shielding wheel is connected to a second motor, and a plurality of second light-shielding sheets are arranged in a circumferential array on the second light-shielding wheel, and the second motor drives the second light-shielding wheel to rotate to switch different second light-shielding sheets to be penetrated by the reflected light of the microfluidic chip.

7. A multimodal observation system for a microfluidic chip according to claim 1, characterized in that, The optical quantitative detection module includes an LED excitation light source, a first filter, a first collimating lens, and a first dichroic mirror; the light emitted by the LED excitation light source is emitted onto the microfluidic chip after passing through the first filter, the first collimating lens, and the first dichroic mirror; the optical quantitative detection module further includes a second collimating lens, a second filter, and a micro detector; the reflected light of the microfluidic chip is collected by the micro detector after passing through the first dichroic mirror, the second collimating lens, and the second filter in sequence.

8. A multimodal observation system for a microfluidic chip according to claim 1, characterized in that, The microscopic observation module includes a second illumination light source, a third collimating lens, a second dichroic mirror, and an objective lens; the light emitted by the second illumination light source is irradiated onto the microfluidic chip after passing through the third collimating lens, the second dichroic mirror, and the objective lens; the microscopic observation module further includes an emission sheet and a second photosensitive coupling component, and the reflected light of the microfluidic chip is collected by the second photosensitive coupling component after passing through the objective lens, the second dichroic mirror, and the emission sheet.

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