Suspension cell culture bioreactor

Abstract

The utility model discloses a suspension cell culture bioreactor which comprises a tank body, the top of the tank body is in threaded connection with a tank cover, the top of the tank cover is fixedly connected with a motor, an output shaft of the motor is fixedly connected with a rotating shaft, the bottom of the rotating shaft penetrates through the tank cover and extends into the tank body, and the surface of the rotating shaft is provided with a plurality of sockets. Through mutual cooperation of the tank body, the tank cover, the motor, the rotating shaft, the insertion opening, the stirring rod, the stop block, the clamping groove, the clamping connection mechanism, a guide groove, a guide column, a sliding plate, a bearing, a screw rod, a handle, a threaded plate and a clamping block, the effect of conveniently disassembling the stirring rod is achieved, the disassembling steps are simple and convenient, time and labor are saved, great convenience is provided for a user, and multiple requirements can be met.

Description

Technical Field

[0001] The utility model relates to the technical field of bioreactors, in particular to a suspension cell culture bioreactor. Background Technique

[0002] A bioreactor refers to a device that provides a good reaction environment for biochemical reactions of cells or microorganisms such as bacteria. Cells grow without depending on the surface of a support and grow in a suspended state in the culture solution. Such cells are called suspension cells. When observed under a microscope, suspension cells generally have a regular cell morphology of round, bright, and uniform-sized cells growing individually, and there are also some cells aggregated and growing in clusters.

[0003] After retrieval, the patent with the application number CN201920865494.8 discloses a stirred stem cell culture bioreactor, belonging to the technical field of biotechnology. A stirred stem cell culture bioreactor includes a reactor body. The upper end of the reactor body is threadedly connected with a cover. A rotating hole is drilled in the upper end of the cover. A rotating cylinder is rotatably connected in the rotating hole. An inner rotating shaft is inserted into the rotating cylinder. The bottom end of the inner rotating shaft is fixedly connected with a turntable. The bottom end of the turntable is fixedly connected with a plurality of uniformly distributed stirring rods. The bottom end of the stirring rod is fixedly connected with a stirring bottom cylinder. The upper end of the inner rotating shaft is fixedly connected with a disc. A compression spring is fixedly connected between the disc and the cover.

[0004] However, in actual use, the above patent has the following defects: it is not convenient to disassemble the stirring rod and it is difficult to meet the needs of multiple parties. For this reason, we propose a suspension cell culture bioreactor. Content of the Utility Model

[0005] The purpose of the utility model is to provide a suspension cell culture bioreactor to solve the problems raised in the above background technique.

[0006] To achieve the above purpose, the utility model provides the following technical solution: A suspension cell culture bioreactor includes a tank body. The top of the tank body is threadedly connected with a tank cover. The top of the tank cover is fixedly connected with a motor. The output shaft of the motor is fixedly connected with a rotating shaft. The bottom of the rotating shaft penetrates the tank cover and extends into the tank body. A plurality of insertion slots are provided on the surface of the rotating shaft. A stirring rod is movably inserted into the insertion slot. Both the left and right ends of the stirring rod penetrate the insertion slot and extend to the outside of the insertion slot. A blocking block is fixedly sleeved on the surface of the stirring rod and on the left side of the rotating shaft. The blocking block is in movable contact with the rotating shaft. Card slots are provided at the top and bottom of the blocking block. A clamping mechanism is arranged on the left side of the rotating shaft.

[0007] Further, the clamping mechanism includes a guide groove, a guide post, a sliding plate, a bearing, a screw rod, a handle, a threaded plate, and a clamping block. Guide grooves are provided on the left side of the rotating shaft and at the top and bottom of the blocking block.

[0008] Further, the top and bottom of the inner wall of the guiding groove are fixedly connected by a guiding column. A sliding plate is slidably connected to one side of the guiding column surface close to the stopper, and the sliding plate is movably connected to the guiding groove.

[0009] Further, the left side of the sliding plate penetrates through the guiding groove and extends to the outside of the guiding groove. A bearing is fixedly connected to one side of the sliding plate away from the stopper, and a screw rod is rotatably connected to one side of the bearing away from the stopper.

[0010] Further, a handle is fixedly connected to one end of the screw rod away from the bearing. A threaded plate is threadedly connected to one side of the screw rod surface close to the handle, and the right side of the threaded plate is fixedly connected to the left side of the rotating shaft.

[0011] Further, a clamping block is fixedly connected to one side of the sliding plate close to the stopper. One side of the clamping block away from the sliding plate penetrates through the clamping groove and extends into the inside of the clamping groove, and the clamping block is movably clamped with the clamping groove.

[0012] Further, a feeding pipe is fixedly communicated with the top of the tank cover, and a discharging pipe is fixedly communicated with the bottom left side of the tank body. Valves are installed on both the feeding pipe and the discharging pipe.

[0013] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0014] Through the mutual cooperation of the tank body, the tank cover, the motor, the rotating shaft, the socket, the stirring rod, the stopper, the clamping groove, the clamping mechanism, the guiding groove, the guiding column, the sliding plate, the bearing, the screw rod, the handle, the threaded plate and the clamping block, the present utility model achieves the effect of facilitating the disassembly of the stirring rod. The disassembly steps are simple, time-saving and labor-saving, providing great convenience for users and meeting the needs of multiple parties. Description of the Drawings

[0015] Figure 1 is the front view structural diagram of the present utility model;

[0016] Figure 2 is the front view structural section of the present utility model;

[0017] Figure 3 is the structural diagram of the stirring rod of the present utility model;

[0018] Figure 4 is the assembly structural diagram of the rotating shaft and the stirring rod of the present utility model.

[0019] In the figure: 1. Tank body; 2. Tank cover; 3. Motor; 4. Rotating shaft; 5. Socket; 6. Stirring rod; 7. Stopper; 8. Card slot; 9. Clamping mechanism; 901. Guide groove; 902. Guide post; 903. Slide plate; 904. Bearing; 905. Screw; 906. Handle; 907. Threaded plate; 908. Clamping block. Detailed implementation mode

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0021] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, unless otherwise stated, the meaning of "plurality" is two or more.

[0022] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0023] Please refer to Figures 1-4, a suspension cell culture bioreactor, including a tank body 1, the top of the tank body 1 is threadedly connected with a tank cover 2, the top of the tank cover 2 is fixedly connected with a motor 3, the output shaft of the motor 3 is fixedly connected with a rotating shaft 4, the bottom of the rotating shaft 4 penetrates through the tank cover 2 and extends into the interior of the tank body 1, a plurality of sockets 5 are opened on the surface of the rotating shaft 4, a stirring rod 6 is movably inserted into the interior of the socket 5, both the left and right ends of the stirring rod 6 penetrate through the socket 5 and extend to the outside of the socket 5, a stopper 7 is fixedly sleeved on the surface of the stirring rod 6 and on the left side of the rotating shaft 4, the stopper 7 is in movable contact with the rotating shaft 4, clamping grooves 8 are opened at both the top and bottom of the stopper 7, and a clamping mechanism 9 is arranged on the left side of the rotating shaft 4.

[0024] Specifically, the clamping mechanism 9 includes a guiding groove 901, a guiding column 902, a sliding plate 903, a bearing 904, a screw rod 905, a handle 906, a threaded plate 907 and a clamping block 908. Guiding grooves 901 are opened at both the top and bottom of the left side of the rotating shaft 4 and on the top and bottom of the stopper 7.

[0025] During specific implementation, the top and bottom of the inner wall of the guiding groove 901 are fixedly connected through the guiding column 902. A sliding plate 903 is slidably connected to the side of the guiding column 902 close to the stopper 7, and the sliding plate 903 is movably connected with the guiding groove 901.

[0026] Specifically, the left side of the sliding plate 903 penetrates through the guiding groove 901 and extends to the outside of the guiding groove 901. A bearing 904 is fixedly connected to the side of the sliding plate 903 away from the stopper 7, and a screw rod 905 is rotatably connected to the side of the bearing 904 away from the stopper 7.

[0027] During specific implementation, a handle 906 is fixedly connected to the end of the screw rod 905 away from the bearing 904. A threaded plate 907 is threadedly connected to the side of the screw rod 905 close to the handle 906, and the right side of the threaded plate 907 is fixedly connected to the left side of the rotating shaft 4.

[0028] Specifically, a clamping block 908 is fixedly connected to the side of the sliding plate 903 close to the stopper 7. The side of the clamping block 908 away from the sliding plate 903 penetrates through the clamping groove 8 and extends into the interior of the clamping groove 8, and the clamping block 908 is movably clamped with the clamping groove 8.

[0029] During specific implementation, a feeding pipe is fixedly communicated with the top of the tank cover 2, a discharge pipe is fixedly communicated with the bottom left side of the tank body 1, and valves are installed on both the feeding pipe and the discharge pipe.

[0030] In practical applications: During use, the culture medium containing suspended cells is introduced into the tank body 1 through the feeding pipe. Then, the motor 3 is started, and the stirring rod 6 is driven by the motor 3 to rotate, thereby stirring the culture medium, and making the suspended cells evenly distributed in the culture medium. When it is necessary to disassemble the stirring rod 6, just rotate the tank cover 2 counterclockwise, so that the tank cover 2 is separated from the tank body 1, and then the stirring rod 6 can be taken out from the tank body 1. Then, just rotate the handle 906 counterclockwise to drive the screw rod 905 to rotate, so that the screw rod 905 moves away from the clamping groove 8 inside the threaded plate 907, so that the clamping block 908 is separated from the clamping groove 8. Then, pull the stirring rod 6 outward, so that the stirring rod 6 is separated from the socket 5, and thus the stirring rod 6 can be removed. Through the above steps, the stirring rod 6 can be conveniently disassembled, with simple operation, saving time and effort.

[0031] All components in the present utility model are common standard parts or parts known to those skilled in the art. Their structures and principles can be known to those skilled in the art through technical manuals or obtained through conventional experimental methods. At the same time, the standard parts used in this application document can be purchased from the market. Each component in this application document can be customized according to the description of the specification and the drawings. The specific connection methods of each part all adopt conventional means such as mature bolts, rivets, and welding in the prior art. The machinery, parts, and equipment all adopt conventional models in the prior art.

[0032] Those skilled in the art connect all the electrical components in this case to their adapted power supplies through wires, and should select appropriate controllers and encoders according to the actual situation to meet the control requirements. For the specific connection and control sequence, reference should be made to the working principle and the sequence of operations of each electrical component to complete the electrical connection. The detailed connection means are well-known techniques in the art. The present utility model mainly introduces the working principle and process, and will not explain the electrical control anymore.

[0033] Although the embodiments of the present utility model have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present utility model. The scope of the present utility model is defined by the appended claims and their equivalents.

Claims

1. A suspension cell culture bioreactor, characterized in that, It includes a tank body (1), a tank cover (2) is threadedly connected to the top of the tank body (1), a motor (3) is fixedly connected to the top of the tank cover (2), a rotating shaft (4) is fixedly connected to the output shaft of the motor (3), the bottom of the rotating shaft (4) penetrates through the tank cover (2) and extends into the interior of the tank body (1), a plurality of socket openings (5) are formed on the surface of the rotating shaft (4), a stirring rod (6) is movably inserted into the interior of the socket opening (5), both the left and right ends of the stirring rod (6) penetrate through the socket opening (5) and extend to the outside of the socket opening (5), a stopper (7) is fixedly sleeved on the surface of the stirring rod (6) and on the left side of the rotating shaft (4), the stopper (7) is in movable contact with the rotating shaft (4), clamping grooves (8) are formed at both the top and bottom of the stopper (7), and a clamping mechanism (9) is arranged on the left side of the rotating shaft (4).

2. The suspension cell culture bioreactor according to claim 1, wherein: The clamping mechanism (9) includes a guiding groove (901), a guiding column (902), a sliding plate (903), a bearing (904), a screw rod (905), a handle (906), a threaded plate (907) and a clamping block (908). Guiding grooves (901) are formed at both the top and bottom of the left side of the rotating shaft (4) and above and below the stopper (7).

3. A suspension cell culture bioreactor according to claim 2, characterized in that: The top and bottom of the inner wall of the guiding groove (901) are fixedly connected by a guiding column (902). A sliding plate (903) is slidably connected to the side of the guiding column (902) close to the stopper (7), and the sliding plate (903) is movably connected to the guiding groove (901).

4. A suspension cell culture bioreactor according to claim 3, characterized in that: The left side of the sliding plate (903) penetrates through the guiding groove (901) and extends to the outside of the guiding groove (901). A bearing (904) is fixedly connected to the side of the sliding plate (903) away from the stopper (7), and a screw rod (905) is rotatably connected to the side of the bearing (904) away from the stopper (7).

5. A suspension cell culture bioreactor according to claim 4, characterized in that: A handle (906) is fixedly connected to the end of the screw rod (905) away from the bearing (904). A threaded plate (907) is threadedly connected to the side of the screw rod (905) close to the handle (906), and the right side of the threaded plate (907) is fixedly connected to the left side of the rotating shaft (4).

6. The suspension cell culture bioreactor according to claim 5, wherein: A clamping block (908) is fixedly connected to the side of the sliding plate (903) close to the stopper (7). The side of the clamping block (908) away from the sliding plate (903) penetrates through the clamping groove (8) and extends into the interior of the clamping groove (8), and the clamping block (908) is movably clamped with the clamping groove (8).

7. The suspension cell culture bioreactor according to claim 6, characterized in that: A feeding pipe is fixedly communicated with the top of the tank cover (2), a discharge pipe is fixedly communicated with the bottom of the left side of the tank body (1), and valves are installed on both the feeding pipe and the discharge pipe.

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