In-vitro differentiation incubator for adipose tissue-derived stem cells

Abstract

The utility model belongs to the technical field of cell culture, and particularly relates to an adipose tissue-derived stem cell in-vitro differentiation culture device which comprises a base body, a top cover, a locking piece and a ventilation mechanism, and can accurately control gas components in a culture environment and maintain sealing performance. The base body is tightly connected with the top cover through the locking piece, so that the stability of the internal environment is ensured; a ventilation mechanism arranged on the top cover is matched with a clamping ring through a limiting groove, so that one-way flowing of gas is achieved, and internal air pressure balance is kept; when a sealing system in the ventilation mechanism stops filling CO2 gas, a ventilation hole can be automatically closed, and gas leakage is prevented; due to the design of the limiting ring and the clamping groove, accurate alignment and stable connection of the top cover and the base body are ensured, and therefore a stable and efficient culture environment is provided for in-vitro differentiation of the adipose-derived mesenchymal stem cells.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and particularly relates to an in vitro differentiation culture device for adipose mesenchymal stem cells. Background Art

[0002] Adipose mesenchymal stem cells (ADSCs) are a type of stem cells with multi-directional differentiation potential and are widely used in tissue engineering, regenerative medicine, and other biomedical fields. ADSCs can differentiate into various cell types under specific conditions, including adipocytes, osteocytes, and chondrocytes. Therefore, the in vitro culture and differentiation technology of ADSCs has become a research hotspot in current biotechnology. However, during the in vitro culture of ADSCs, the cell growth environment, such as gas components, humidity, and temperature, all have a significant impact on the proliferation and differentiation effects of cells.

[0003] Existing cell culture equipment usually cannot accurately control the CO2 concentration in the culture environment, which easily leads to uneven gas components during the culture process and thus affects the differentiation effect of ADSCs. In addition, the sealing performance of existing equipment is poor, which easily causes gas leakage and makes the culture environment unstable. To solve these problems, it has become an urgent technical problem to provide an in vitro differentiation culture device for adipose mesenchymal stem cells that can accurately control the CO2 concentration and has good sealing performance. Summary of the Utility Model

[0004] Aiming at the problems existing in the prior art, the purpose of the utility model is to provide an in vitro differentiation culture device for adipose mesenchymal stem cells, which can achieve accurate control of the CO2 concentration in the internal space during cell culture and maintain the sealing of the culture space at the same time, thereby providing a stable culture environment for the in vitro differentiation of adipose mesenchymal stem cells and effectively improving the efficiency and quality of cell differentiation.

[0005] To achieve the above purpose, the utility model provides the following technical solutions:

[0006] An in vitro differentiation culture device for adipose mesenchymal stem cells, including a base body and a top cover buckled on the base body. A locking member for fixing the base body and the top cover is clamped on the outer side of the base body, and the locking members are evenly distributed at equal intervals around the central axis of the base body;

[0007] A plurality of through holes are penetrated through the top of the top cover, and a ventilation mechanism for controlling the unidirectional flow of gas is clamped in the through holes.

[0008] Further, the ventilation mechanism includes a positioning tube inserted into the through hole;

[0009] A clamping ring is fixedly connected to the inner wall of the through hole;

[0010] On the outer side of the positioning tube, limiting grooves adapted to the clamping rings are symmetrically provided.

[0011] Furthermore, a retaining ring for limiting the position of the positioning tube is fixedly connected to the outer side of the middle part of the positioning tube.

[0012] Furthermore, a baffle is fixedly connected to the middle part of the inner side of the positioning tube, and a positioning member is fixedly connected to one side of the baffle;

[0013] One end of the positioning member is provided with a sealing cavity penetrating therethrough;

[0014] A sealing plug is inserted into the sealing cavity, one end of the sealing plug is connected with a guide post, and a spring is sleeved on the guide post.

[0015] Furthermore, the shape of the sealing cavity is set to be funnel-shaped, and the shape of the sealing plug is the same as that of the sealing cavity.

[0016] Furthermore, ventilation holes are penetrated through the inner wall of the positioning member, and the ventilation holes are equidistantly distributed around the central axis of the positioning member.

[0017] Furthermore, a threaded groove is provided at the top of the sealing plug, a threaded post adapted to the threaded groove is fixedly connected to the bottom end of the guide post, and a retaining disc is fixedly connected to the top end of the guide post.

[0018] Furthermore, the diameter of the guide post is the same as the inner diameter of the top of the sealing cavity.

[0019] Furthermore, a limiting ring is fixedly connected to the top of the base body, and the outer diameter of the limiting ring is the same as the inner diameter of the top cover.

[0020] Furthermore, a clamping groove is provided on the outer side of the base body;

[0021] A positioning protrusion adapted to the clamping groove is fixedly connected to the side surface of the locking member;

[0022] And a lower pressing plate is fixedly connected to the top of the locking member.

[0023] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0024] Firstly, through the setting of the locking member between the base body and the top cover, efficient sealing of the incubator is achieved. The locking members are evenly distributed on the outer side of the base body, and can effectively connect the base body and the top cover tightly together, avoiding leakage during the charging of CO2 gas. This design ensures the stability of the internal space of the incubator, thereby providing an independent and closed environment for the in vitro differentiation of adipose mesenchymal stem cells and improving the success rate of cell culture.

[0025] Secondly, through the multiple through-holes opened on the top cover and the air-permeable mechanism installed in the through-holes, the present utility model can precisely control the unidirectional flow of gas inside the incubator. The positioning tube in the air-permeable mechanism is cooperated with the clamping ring on the inner wall of the through-hole, realizing the stable installation of the air-permeable mechanism in the through-hole, thus preventing the loosening or detachment of the air-permeable mechanism when gas is filled. This design ensures that CO2 gas can uniformly enter the culture space, while the excess gas can be discharged through the reversely installed air-permeable mechanism, maintaining the balance of the internal air pressure, thereby providing the best gas environment for cell growth.

[0026] Thirdly, the sealing system composed of the positioning member, the sealing cavity and the sealing plug designed in the present utility model can quickly close the air-permeable hole through the action of the spring when the CO2 gas filling stops, ensuring the airtightness of the culture space. The funnel-shaped design of the sealing cavity and the sealing plug further improves the sealing effect, effectively avoiding the problem of gas leakage. This design not only ensures the stability of the environment inside the incubator, but also prevents the interference of the external environment on the culture process, thus significantly improving the culture quality and differentiation efficiency of adipose mesenchymal stem cells.

[0027] Finally, the design of the limiting ring, the clamping groove and the positioning protrusion in the present utility model ensures the precise alignment and firm connection of the top cover and the base body. The setting of the limiting ring provides an effective positioning function during the installation of the top cover, making the installation process simple and accurate, and further enhancing the sealing performance of the incubator. In addition, the tight fit of the clamping groove and the positioning protrusion avoids the loosening or sliding of the locking member during use, thereby further ensuring the overall stability and service life of the incubator. In summary, through multi-faceted structural optimization and design improvement, the present utility model effectively solves the deficiencies in gas control and sealing performance of the existing adipose mesenchymal stem cell incubator, providing a more stable and efficient environment for the in vitro differentiation of adipose mesenchymal stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a structural schematic diagram of the present utility model;

[0029] Figure 2 is a structural schematic diagram of the base body of the present utility model;

[0030] Figure 3 is a structural schematic diagram of the top cover of the present utility model;

[0031] Figure 4 is a structural schematic diagram of the locking member of the present utility model;

[0032] Figure 5 is a structural schematic of the air-permeable mechanism of the present utility model Figure 1 ;

[0033] Figure 6Structural schematic of the ventilation mechanism of the present utility model Figure 2 。

[0034] In the attached drawings, the list of components represented by each reference numeral is as follows:

[0035] 1. Substrate; 11. Clamping groove; 12. Limiting ring;

[0036] 2. Top cover; 21. Through hole; 211. Clamping ring;

[0037] 3. Locking member; 31. Lower pressing plate; 32. Positioning protrusion;

[0038] 4. Ventilation mechanism;

[0039] 41. Positioning tube; 411. Limiting groove; 412. Stop ring;

[0040] 42. Baffle;

[0041] 43. Positioning member; 431. Sealing cavity; 432. Ventilation hole;

[0042] 44. Sealing plug; 441. Threaded groove;

[0043] 45. Guide post; 451. Stop disc; 452. Threaded post; 46. Spring. Detailed implementation manners

[0044] In order to make the purpose and advantages of the present utility model clearer, the present utility model will be specifically described below in conjunction with embodiments. It should be understood that the following text is only used to describe one or several specific implementation manners of the present utility model, and does not strictly limit the specific protection scope claimed by the present utility model.

[0045] Refer to Figures 1-6 , a device for in vitro differentiation and culture of adipose mesenchymal stem cells, comprising a substrate 1 and a top cover 2 buckled on the substrate 1; a locking member 3 for fixing the substrate 1 and the top cover 2 is clamped on the outside of the substrate 1, and the locking members 3 are evenly distributed around the central axis of the substrate 1; through the setting of the locking member 3, the tight connection between the substrate 1 and the top cover 2 can be effectively ensured, thereby improving the sealing performance of the device and avoiding leakage during the injection of CO2 gas; a plurality of through holes 21 are formed through the top of the top cover 2, and a ventilation mechanism 4 for controlling the unidirectional flow of gas is clamped in the through holes 21; the setting of these ventilation mechanisms 4 ensures that the flow of CO2 gas can be precisely controlled during the cell culture process, so that the gas composition in the culture environment remains stable, thereby providing good conditions for the in vitro differentiation of adipose mesenchymal stem cells.

[0046] Refer to Figure 3 and Figure 5, the ventilation mechanism 4 includes a positioning tube 41 inserted into the through hole 21; the design of the positioning tube 41 can ensure the stability of the ventilation mechanism 4 within the through hole 21, preventing the ventilation mechanism 4 from loosening or detaching due to air pressure changes when filling with CO2 gas; a clamping ring 211 is fixedly connected to the inner wall of the through hole 21; the clamping ring 211 can cooperate with the limiting groove 411 on the positioning tube 41 to achieve reliable positioning of the ventilation mechanism 4 within the through hole 21; limiting grooves 411 adapted to the clamping ring 211 are symmetrically formed on the outer side surface of the positioning tube 41; the symmetrical design of the limiting grooves 411 not only enhances the stability of the ventilation mechanism 4 but also makes installation and disassembly more convenient, facilitating the improvement of the overall usage efficiency of the device.

[0047] Refer to Figures 5-6 , a retaining ring 412 for limiting the position of the positioning tube 41 is fixedly connected to the outer side of the middle part of the positioning tube 41; the provision of the retaining ring 412 can effectively limit the axial movement of the positioning tube 41 within the through hole 21, ensuring the stability of the ventilation mechanism 4, so that when gas is filled or discharged, the positioning tube 41 will not be displaced due to air pressure fluctuations, further enhancing the reliability of the device. A baffle 42 is fixedly connected to the middle part of the inner side of the positioning tube 41, and a positioning member 43 is fixedly connected to one side of the baffle 42; a sealing cavity 431 is formed through the end of the positioning member 43; a sealing plug 44 is inserted into the sealing cavity 431, one end of the sealing plug 44 is connected to a guide post 45, and a spring 46 is sleeved on the guide post 45; through the provision of the spring 46, when stopping the filling of CO2 gas, the spring 46 can quickly push the guide post 45, causing the sealing plug 44 to closely fit the inner wall of the sealing cavity 431, thereby effectively sealing the ventilation hole 432 and ensuring the airtightness of the culture space.

[0048] Refer to Figures 5-6 , the shape of the sealing cavity 431 is set as a funnel shape, and the shape of the sealing plug 44 is the same as that of the sealing cavity 431; this design can ensure a tighter fit between the sealing plug 44 and the sealing cavity 431, further improving the sealing effect of the ventilation hole 432; through such a design, it is possible to effectively avoid gas leakage problems caused by poor sealing after stopping the filling of CO2 gas, ensuring the stability of the culture environment.

[0049] Refer to Figures 5-6 , ventilation holes 432 are formed through the inner wall of the positioning member 43, and the ventilation holes 432 are evenly distributed at equal intervals around the central axis of the positioning member 43; the uniform distribution of the ventilation holes 432 can ensure that the CO2 gas is evenly distributed when entering the culture space, thereby maintaining a consistent gas concentration throughout the culture space, which is crucial for the in vitro differentiation process of adipose mesenchymal stem cells and is conducive to improving the success rate and quality of cell culture.

[0050] Refer to Figure 5, a threaded groove 441 is provided at the top of the sealing plug 44, the bottom end of the guiding column 45 is fixedly connected with a threaded post 452 adapted to the threaded groove 441, and the top end of the guiding column 45 is fixedly connected with a retaining disc 451; through the cooperation of the threaded groove 441 and the threaded post 452, the guiding column 45 can accurately move along the inner wall of the sealing cavity 431 under the action of the spring 46; the setting of the retaining disc 451 can effectively prevent the guiding column 45 from deviating from the central axis during the movement, ensuring the tight fit between the sealing plug 44 and the sealing cavity 431 and improving the sealing performance of the device.

[0051] Refer to Figure 6 , the diameter of the guiding column 45 is the same as the inner diameter of the top of the sealing cavity 431; such a design ensures that the guiding column 45 always remains at the central position of the sealing cavity 431 during the movement, so that the sealing effect between the sealing plug 44 and the sealing cavity 431 is more reliable, effectively avoiding the problem of poor sealing caused by the movement deviation of the guiding column 45.

[0052] Refer to Figures 2-3 , a limiting ring 12 is fixedly connected to the top of the base body 1, and the outer diameter of the limiting ring 12 is the same as the inner diameter of the top cover 2; the setting of the limiting ring 12 can play a positioning role when the top cover 2 is buckled with the base body 1, ensuring that the top cover 2 can accurately align with the position of the base body 1 during installation, thereby improving the installation accuracy of the device and further enhancing the sealing performance of the incubator.

[0053] Refer to Figures 1-4 , a clamping groove 11 is provided on the outer side of the base body 1; a positioning protrusion 32 adapted to the clamping groove 11 is fixedly connected to the side of the locking member 3; through the cooperation of the clamping groove 11 and the positioning protrusion 32, the locking member 3 can be effectively prevented from sliding or loosening during use, ensuring the tight connection between the base body 1 and the top cover 2 and avoiding leakage during the CO2 gas filling process; and a lower pressing plate 31 is fixedly connected to the top of the locking member 3; the setting of the lower pressing plate 31 can provide additional pressure when the locking member 3 is connected to the base body 1 and the top cover 2, thereby further enhancing the sealing performance of the device and improving the overall stability of the incubator.

[0054] The working principle of the present utility model is as follows:

[0055] When culturing cells, first put the culture medium and the cells to be cultured into the base body 1 in a specific proportion;

[0056] Then install the air-permeable mechanism 4 in the through hole 21 provided on the top cover 2. When installing the air-permeable mechanism 4, the air-permeable mechanism 4 should be installed forward in the central through hole 21, and the air-permeable mechanism 4 should be installed backward in the remaining through holes 21;

[0057] After the ventilation mechanism 4 is installed, the top cover 2 is buckled on the base body 1, and the locking member 3 is used to lock the base body 1 and the top cover 2 together;

[0058] When it is necessary to fill 5% CO2 into the space formed by the base body 1 and the top cover 2, only need to fill CO2 from the ventilation mechanism 4 at the center of the top cover 2;

[0059] When the CO2 gas enters the internal space of the positioning tube 41 and the baffle 42, at this time, under the action of air pressure, it will push the sealing plug 44 away from the sealing cavity 431, and then let the CO2 gas enter the internal space of the base body 1 and the top cover 2 through the ventilation holes 432;

[0060] When filling CO2, the internal pressure of the base body 1 and the top cover 2 will increase, and the excess gas will be discharged through the other reversely installed ventilation mechanisms 4, so that the pressure in the internal space of the base body 1 and the top cover 2 is in a balanced state;

[0061] When stopping filling CO2 gas, at this time, under the push of the spring 46, the guide post 45 will drive the sealing plug 44 to move, so that the sealing plug 44 fits against the inner wall of the sealing cavity 431, thereby sealing and blocking the ventilation holes 432. In this way, the space formed by the base body 1 and the top cover 2 is an independent sealed space.

[0062] The above is only the preferred embodiment of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and retouches can be made, and these improvements and retouches should also be regarded as the protection scope of the present invention. The structures, devices and operation methods not specifically described and explained in the present invention, unless otherwise specified and limited, are implemented according to the conventional means in the art.

Claims

1. An in vitro differentiation culture device for adipose mesenchymal stem cells, comprising a base body (1) and a top cover (2) buckled on the base body (1), characterized in that: A locking member (3) for fixing the base body (1) and the top cover (2) is engaged on the outer side of the base body (1), and the locking members (3) are evenly distributed around the central axis of the base body (1); A plurality of through holes (21) are formed through the top of the top cover (2), and a ventilation mechanism (4) for controlling the unidirectional flow of gas is engaged in the through holes (21).

2. The adipose mesenchymal stem cell in vitro differentiation culture device according to claim 1, characterized in that: The ventilation mechanism (4) includes a positioning tube (41) inserted into the through hole (21); A clamping ring (211) is fixedly connected to the inner wall of the through hole (21); Limiting grooves (411) adapted to the clamping ring (211) are symmetrically formed on the outer side surface of the positioning tube (41).

3. The adipose mesenchymal stem cell in vitro differentiation culture device according to claim 2, wherein: A retaining ring (412) for limiting the position of the positioning tube (41) is fixedly connected to the outer side of the middle part of the positioning tube (41).

4. The adipose mesenchymal stem cell in vitro differentiation culture apparatus according to claim 2, characterized in that: A baffle (42) is fixedly connected to the middle part inside the positioning tube (41), and a positioning member (43) is fixedly connected to one side of the baffle (42); A sealing cavity (431) is formed through one end of the positioning member (43); A sealing plug (44) is inserted into the sealing cavity (431), one end of the sealing plug (44) is connected with a guide post (45), and a spring (46) is sleeved on the guide post (45).

5. The adipose mesenchymal stem cell in vitro differentiation culture apparatus according to claim 4, characterized in that: The shape of the sealing cavity (431) is set to be funnel-shaped, and the shape of the sealing plug (44) is the same as that of the sealing cavity (431).

6. The in vitro differentiation culture device for adipose mesenchymal stem cells according to claim 5, characterized in that: Ventilation holes (432) are formed through the inner wall of the positioning member (43), and the ventilation holes (432) are evenly distributed around the central axis of the positioning member (43).

7. The adipose mesenchymal stem cell in vitro differentiation culture device according to claim 5, characterized in that: A threaded groove (441) is formed in the top of the sealing plug (44), a threaded post (452) adapted to the threaded groove (441) is fixedly connected to the bottom end of the guide post (45), and a retaining disc (451) is fixedly connected to the top end of the guide post (45).

8. The adipose mesenchymal stem cell in vitro differentiation culture apparatus according to claim 7, characterized in that The diameter of the guide post (45) is the same as the inner diameter of the top of the sealing cavity (431).

9. The adipose mesenchymal stem cell in vitro differentiation culture device according to claim 1, characterized in that: A limiting ring (12) is fixedly connected to the top of the base body (1), and the outer diameter of the limiting ring (12) is the same as the inner diameter of the top cover (2).

10. The adipose mesenchymal stem cell in vitro differentiation culture apparatus according to claim 9, characterized in that: A clamping groove (11) is formed on the outer side of the base body (1); A positioning protrusion (32) adapted to the clamping groove (11) is fixedly connected to the side surface of the locking member (3); And a lower pressing plate (31) is fixedly connected to the top of the locking member (3).

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