Smear making mechanism and device

Abstract

The utility model discloses a smear manufacturing mechanism and device, and relates to the technical field of medical instruments. The smear manufacturing mechanism comprises a feeding assembly, a spraying assembly and a conveying assembly, the feeding assembly comprises a storage piece and a liquid conveying piece, and the storage piece is used for storing a liquid sample; the spraying assembly comprises a spraying piece, and the liquid conveying piece is connected with the storage piece and the spraying piece; the conveying assembly is provided with a smear station, the conveying assembly is used for conveying a glass slide to the smear station, and the spraying piece is arranged corresponding to the smear station, so that a liquid sample sprayed by the spraying piece is attached to the glass slide, and a sample film is formed. The smear manufacturing mechanism provided by the utility model is simpler and more compact in structure.

Description

Technical Field

[0001] This application relates to the technical field of medical devices, and particularly to a smear making mechanism and device. Background Art

[0002] When making a smear, a sample needs to be dropped on a clean glass slide for staining. Existing fully automatic blood cell smearers usually have a sample dropping mechanism and a spreading mechanism. During slide making, the sample is first dropped on the glass slide through the sample dropping component, and then the spreading mechanism is used to spread the sample liquid drop on the glass slide to achieve the purpose of simulating manual smear making.

[0003] However, the setting of the sample dropping mechanism and the spreading mechanism makes the mechanical components of the fully automatic blood cell smearer numerous and complex, so the device has a large volume. Summary of the Utility Model

[0004] In view of this, this application provides a smear making mechanism and device, aiming to solve one of the technical problems in the prior art.

[0005] To achieve the above object, the technical solution adopted in this application is as follows:

[0006] In the first aspect, an embodiment of this application provides a smear making mechanism, including:

[0007] A feeding component, including a storage part and an infusion part, where the storage part is used to store a liquid sample;

[0008] A spraying component, including a spraying part, and the infusion part is connected to the storage part and the spraying part;

[0009] A conveying component, provided with a smear making station, and the conveying component is used to convey a glass slide to the smear making station. The spraying part is arranged corresponding to the smear making station, so that the liquid sample sprayed by the spraying part adheres to the glass slide and forms a sample film.

[0010] In one of the embodiments of the first aspect, the spraying part includes:

[0011] A movable part;

[0012] At least one nozzle, the nozzle is communicated with the infusion part, the movable part is connected to the nozzle, and the movable part moves to make the sample liquid in the nozzle spray out.

[0013] In one of the embodiments of the first aspect, the movable part is a piezoelectric movable part, and the spraying part further includes a driving part, and the driving part drives the movable part to move.

[0014] In one embodiment of the first aspect, the spraying assembly further includes a moving member, the nozzle is connected to the moving member, and the moving member drives the nozzle to move perpendicular to the conveying direction of the slide.

[0015] In one embodiment of the first aspect, the liquid infusion member includes:

[0016] A sheath flow chamber provided with a liquid inlet and a liquid outlet;

[0017] A sampling needle which penetrates through the sheath flow chamber and is located in the middle of the sheath flow chamber.

[0018] In one embodiment of the first aspect, the liquid infusion member further includes a suction pump, and the suction pump communicates with the storage member and the sampling needle.

[0019] In one embodiment of the first aspect, a focusing portion is formed at the liquid outlet end of the sheath flow chamber, and the liquid outlet of the sheath flow chamber is provided at the bottom of the focusing portion.

[0020] In one embodiment of the first aspect, the inner diameter of the focusing portion decreases in the direction close to the liquid outlet of the sheath flow chamber.

[0021] In one embodiment of the first aspect, the needle tip of the sampling needle is located in the middle of the focusing portion.

[0022] In a second aspect, an embodiment of the present application further provides a smear making device, including the smear making mechanism in any of the above embodiments.

[0023] Compared with the prior art, the beneficial effect of the present application is that the present application provides a smear making mechanism, including a feeding assembly, a spraying assembly and a conveying assembly. The feeding assembly includes a storage member and a liquid infusion member, and the storage member is used for storing a liquid sample; the spraying assembly includes a spraying member, and the liquid infusion member connects the storage member and the spraying member; the conveying assembly is provided with a smear station, and the conveying assembly is used for conveying a slide to the smear station, and the spraying member is arranged corresponding to the smear station so that the liquid sample ejected by the spraying member adheres to the slide and forms a sample film. In this way, the mechanical structure of the smear making mechanism can be reduced, the smear making mechanism can be simplified as a whole, the layout can be more compact, and the volume can be reduced. Description of the Drawings

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings required to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without creative efforts.

[0025] Figure 1 Shows a schematic structural diagram of a smear-making mechanism in some embodiments of the present application;

[0026] Figure 2 Shows a schematic structural diagram of a spraying member in some embodiments of the present application;

[0027] Figure 3 Shows a schematic structural diagram of an infusion member in some embodiments of the present application.

[0028] Description of main element symbols: 100 - smear-making mechanism; 110 - feeding assembly; 111 - storage member; 112 - infusion member; 120 - spraying assembly; 130 - conveying assembly; 140 - glass slide; 121 - spraying member; 1211 - nozzle; 1212 - movable part; 1213 - driving part; 1121 - sheath flow chamber; 11211 - focusing part; 1122 - sampling needle; D1 - moving direction of the glass slide; P1 - smear station. Detailed description of specific embodiments

[0029] The embodiments of the present application will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present application and should not be construed as a limitation of the present application.

[0030] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present application.

[0031] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality" means two or more unless otherwise specifically defined.

[0032] In this application, unless otherwise clearly defined or limited, terms such as "install", "connect", "link", "fix", etc. shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral one; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0033] In this application, unless otherwise clearly defined or limited, the first feature being "on" or "under" the second feature may be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on top of" the second feature may be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "underneath" the second feature may be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0034] Existing fully automatic blood cell smearers usually have a sample dropping mechanism and a spreading mechanism. When making a smear, the sample is first dropped on a glass slide through the sample dropping component, and then the sample liquid drop on the glass slide is spread flat by the spreading mechanism. Such a structure makes the mechanical components of the fully automatic blood cell smearer more and more complex, so the equipment volume is relatively large.

[0035] In view of the above problems, as Figure 1 shown, an embodiment of this application provides a smear making mechanism 100, which is mainly used for making blood cell smears. The smear making mechanism 100 includes a feeding component 110, a spraying component 120 and a conveying component 130.

[0036] Among them, the feeding component 110 includes a storage part 111 and an infusion part 112. The storage part 111 is used for storing liquid samples; the spraying component 120 includes a spraying part 121, and the infusion part 112 connects the storage part 111 and the spraying part 121; the conveying component 130 is provided with a smear station P1, and the conveying component 130 is used for conveying the glass slide 140 to the smear station P1. The spraying part 121 is arranged corresponding to the smear station P1, so that the liquid sample sprayed by the spraying part 121 adheres to the glass slide 140 and forms a sample film. In this way, the mechanical structure of the smear making mechanism 100 can be reduced, so that the smear making mechanism 100 is simplified as a whole, the layout is more compact, and the volume is reduced.

[0037] In some embodiments, the storage member 111 is an ordinary biological sample container. Reagents can be added into the sample container to preliminarily process the biological sample without adding a vibration device, a heating device, a stirring device, etc., which simplifies the structure of the smear making mechanism 100 and reduces the volume.

[0038] In some embodiments, as Figure 2 shown, the spraying member 121 includes a movable part 1212 and at least one nozzle 1211.

[0039] In this embodiment, the number of nozzles 1211 is one.

[0040] In other embodiments, the number of nozzles 1211 is two, three, four, etc., which is not limited to the exemplified numbers.

[0041] It can be understood that when the number of nozzles 1211 is multiple, the multiple nozzles 1211 are arranged at intervals along a direction parallel or perpendicular to the conveying direction of the glass slide 140, or are arranged at intervals in a circular or rectangular shape.

[0042] It should be noted that the arrangement mode of the multiple nozzles 1211 can be adjusted according to the spraying process or the spraying area of the nozzles 1211.

[0043] For example, when the spraying process is that the glass slide 140 is stationary while the nozzle 1211 moves, the multiple nozzles 1211 are arranged at intervals along a direction parallel or perpendicular to the conveying direction of the glass slide 140 and move along a direction perpendicular or parallel to the conveying direction of the glass slide 140, so as to form a sample film on the entire surface of the glass slide 140. This nozzle 1211 arrangement mode is also applicable to the case where the nozzle 1211 is stationary while the glass slide 140 moves, and will not be repeated here.

[0044] For example, when the spraying process is that neither the nozzle 1211 nor the glass slide 140 moves, when the multiple nozzles 1211 are arranged in a circular or rectangular shape, the multiple nozzles 1211 spray simultaneously, and the total spraying area is close to the surface of the glass slide 140. In this way, without setting a subsequent moving member, only by setting the conveying assembly 130 to move the glass slide 140 to the smear making station P1, a sample film can be quickly formed on the surface of the glass slide 140, improving the preparation efficiency. It should be understood that when the spraying area of one nozzle 1211 meets the use requirements or is close to the surface area of the glass slide 140, only one nozzle 1211 can be set.

[0045] In some embodiments, the nozzle 1211 is communicated with the liquid infusion member 112 to enable the liquid sample to enter the nozzle 1211. The movable part 1212 is connected to the nozzle 1211, and the movable part 1212 moves to eject the sample liquid in the nozzle 1211.

[0046] Exemplarily, when the movable part 1212 is arranged inside the nozzle 1211, the movable part 1212 is a piston structure. The liquid sample is pressed towards the outlet of the nozzle 1211 by the piston, so as to form tiny droplets adhering to the surface of the glass slide 140. In this way, it is necessary to enlarge the volume of the nozzle 1211 so that the nozzle 1211 can accommodate the piston.

[0047] It should be noted that the tiny droplets are beneficial to keeping the cells in the liquid sample ejected from the nozzle 1211 intact and avoiding damage to the cell structure.

[0048] Exemplarily, when the movable part 1212 is arranged outside the nozzle 1211, the movable part 1212 is a piezoelectric structure. The side wall of the nozzle 1211 is squeezed by the piezoelectric structure, and the sample liquid is pressed out of the outlet of the nozzle 1211, so as to form tiny droplets adhering to the surface of the glass slide 140. Compared with arranging a piston structure inside the nozzle 1211, arranging a piezoelectric structure is beneficial to reducing the volume of the nozzle 1211.

[0049] In this embodiment, the movable part 1212 is a piezoelectric movable part, and the spraying part 121 further includes a driving part 1213, and the driving part 1213 drives the movable part 1212 to move.

[0050] In some embodiments, the piezoelectric movable part 1212 is made of piezoelectric ceramics.

[0051] The working mode of the driving part 1213 driving the piezoelectric ceramics is as follows:

[0052] The external controller inputs a working signal to the driving part 1213, and the driving part 1213 changes the charge distribution inside the piezoelectric ceramics, so that the piezoelectric ceramics generate deformation, apply pressure to the sample liquid inside the nozzle 1211, convert electrical energy into mechanical energy, and make the liquid sample be ejected from the nozzle 1211 quickly.

[0053] It should be understood that the deformation conditions, frequencies and times of the piezoelectric ceramics can be set by the external controller.

[0054] For example, the external controller can synchronously control the moving speed of the glass slide 140 on the conveying component 130, so that after each glass slide 140 moves to the smearing station P1, it controls the piezoelectric ceramics to generate deformation until the spraying process is completed.

[0055] In some embodiments, the spraying component 120 further includes a moving part (not shown in the figure), the nozzle 1211 is connected to the moving part, and the moving part drives the nozzle 1211 to move along a direction perpendicular to the conveying direction of the glass slide 140.

[0056] In this embodiment, the number of nozzles 1211 is one, and the moving part drives the nozzle 1211 to reciprocate along the advancing direction perpendicular to the glass slide 140 until a sample film is formed on the entire surface of the glass slide 140.

[0057] It should be understood that the capacity of the nozzle 1211 is small, so the spraying area of the nozzle 1211 is less than or equal to the surface area of the glass slide 140.

[0058] In this embodiment, the spraying process is as follows:

[0059] The conveying assembly 130 drives the glass slide 140 to pass through the smear station P1 at a constant speed, and the nozzle 1211 reciprocates along the width direction of the glass slide 140 to form a sample film on the entire surface of the glass slide 140.

[0060] The inventor found that in the existing fully automatic blood cell smearer, when making a smear, the sample is first dropped on the glass slide by the sample dropping assembly, and then the sample liquid drop on the glass slide is pushed flat by the slide pushing mechanism, so as to form a sample film on the glass slide.

[0061] However, the small-volume cells in the sample film formed by the mechanical slide pushing method are relatively evenly distributed, while the large-volume cells are pushed to the periphery of the sample film along with the slide pushing action, that is, there is a problem of uneven distribution of large and small cells in the sample film in the mechanical slide pushing method.

[0062] In view of the above problems, in some embodiments, the infusion member 112 includes a sheath flow chamber 1121 and a sampling needle 1122.

[0063] As Figure 3 shown, the sheath flow chamber 1121 is provided with a liquid inlet and a liquid outlet, and the sampling needle 1122 penetrates through the sheath flow chamber 1121 from the top of the sheath flow chamber 1121 and is located in the middle of the sheath flow chamber 1121.

[0064] In some embodiments, the infusion member 112 further includes a suction pump (not shown in the figure), and the suction pump is communicated with the storage member 111 and the sampling needle 1122.

[0065] In some embodiments, as Figure 3 shown, a focusing portion 11211 is formed at the liquid outlet end of the sheath flow chamber 1121, the liquid outlet of the sheath flow chamber 1121 is provided at the bottom of the focusing portion 11211, and the inner diameter of the focusing portion 11211 decreases in the direction close to the liquid outlet of the sheath flow chamber 1121.

[0066] Specifically, the sheath liquid enters the sheath flow chamber 1121 under a certain pressure, and the sheath flow forms a stable liquid flow after continuous flow through the sheath flow chamber 1121. When the liquid flow flows to the focusing portion 11211, due to the fact that the inner diameter of the focusing portion 11211 decreases in the direction close to the liquid outlet of the sheath flow chamber 1121, the sheath flow forms a fluid focus in the focusing portion 11211.

[0067] In some embodiments, the needle tip of the sampling needle 1122 is located in the middle of the focusing portion 11211.

[0068] The liquid sample forms a sample flow, which is focused into a liquid flow with a very small inner diameter when flowing out from the tip of the sampling needle 1122. The sampling needle 1122 is located at the central axis of the sheath flow chamber 1121, and the tip of the sampling needle 1122 is located in the middle of the fluid focusing part 11211.

[0069] By arranging the sheath flow chamber 1121 and the sampling needle 1122, the sample flow formed by the liquid sample in the sampling needle 1122 and the sheath liquid flowing out from all around flow through the nozzle 1211 together, so that the cell suspension forms a single-arranged cell flow in the middle, surrounded by the sheath liquid all around.

[0070] Under the condition of satisfying the "Reynolds transport theorem", the sample flow and the sheath flow are in a laminar flow state. By adjusting the sample flow velocity and the sheath flow velocity, the sheath flow can wrap the sample flow so that the cells in the sample flow are arranged neatly one by one, thereby obtaining the required sample flow diameter and sample flow.

[0071] When the nozzle 1211 ejects the sample liquid, the formed sample film has a large size and the cells of large and small volumes are evenly distributed.

[0072] In addition, the glass slide 140 is usually made of an alkaline glass material. Before mechanical spreading, the surface of the glass slide 140 needs to be cleaned. Otherwise, during the spreading process, air bubbles are likely to be generated in the sample film.

[0073] The smear-making mechanism 100 of the present application, through the structures of the sheath flow chamber 1121 and the sampling needle 1122, dilutes the cells in the liquid sample with the sheath liquid, and then forms a single-cell sequential sorting method to input into the nozzle 1211, and then sprays the liquid sample on the surface of the glass slide 140 through the nozzle 1211 to form a sample film. Such a processing technology will not generate air bubbles in the sample film and has good uniformity in the distribution of each cell.

[0074] The smear-making mechanism 100 of the present application can also control the entire process of blood cell ejection through the moving part 1212, accurately control the ejection area size and ejection position of the sample liquid, and avoid the problem of blood cell overlap.

[0075] The present application also provides a smear-making device, including the smear-making mechanism 100 in any of the above embodiments. Therefore, it has all the beneficial effects of the smear-making mechanism 100 in any of the above embodiments, which will not be elaborated here one by one.

[0076] In the description of this specification, the description with reference to terms such as "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of this application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, without contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples.

[0077] Although the embodiments of this application have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limitations on this application. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of this application.

Claims

1. A smear-making mechanism, characterized in that, Comprising: A feeding component, including a storage part and an infusion part, where the storage part is used for storing liquid samples; A spraying component, including a spraying part, and the infusion part connects the storage part and the spraying part; A conveying component, provided with a smear station, and the conveying component is used for conveying a glass slide to the smear station, and the spraying part is arranged corresponding to the smear station, so that the liquid sample ejected by the spraying part adheres to the glass slide and forms a sample film.

2. The smear preparation mechanism according to claim 1, wherein, The spraying part includes: A movable part; At least one nozzle, the nozzle is communicated with the infusion part, the movable part is connected with the nozzle, and the movable part moves to eject the sample liquid in the nozzle.

3. The smear-making mechanism according to claim 2, wherein, The movable part is a piezoelectric movable part, and the spraying component further includes a driving part, and the driving part drives the movable part to move.

4. The smear-making mechanism according to claim 2, wherein The spraying component further includes a moving part, the nozzle is connected with the moving part, and the moving part drives the nozzle to move in a direction perpendicular to the conveying direction of the glass slide.

5. The smear-making mechanism according to any one of claims 1 to 4, characterized in that, The infusion part includes: A sheath flow chamber, provided with a liquid inlet and a liquid outlet; A sampling needle, the sampling needle penetrates through the sheath flow chamber and is located in the middle of the sheath flow chamber.

6. The smear-making mechanism according to claim 5, characterized in that, The infusion part further includes a suction pump, and the suction pump is communicated with the storage part and the sampling needle.

7. The smear preparation mechanism according to claim 5, wherein A focusing part is formed at the liquid outlet end of the sheath flow chamber, and the liquid outlet of the sheath flow chamber is arranged at the bottom of the focusing part.

8. The smear-making mechanism according to claim 7, characterized in that, The inner diameter of the focusing part decreases in the direction close to the liquid outlet of the sheath flow chamber.

9. The smear-making mechanism according to claim 7, characterized in that, The needle tip of the sampling needle is located in the middle of the focusing part.

10. A smear-making device, characterized in that, Including the smear making mechanism according to any one of claims 1 to 9.

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