Microplate reader capable of automatically adding buffer solution

Abstract

A microplate reader capable of automatically adding a buffer solution comprises an electric linear sliding table, a microplate reader body, a buffer solution tank, an electric push rod, a solution suction device, a vibration motor and a control circuit, the electric linear sliding table is mounted at the rear upper end in a shell of the microplate reader body, the supporting frame is mounted at the lower end of a sliding block of the electric linear sliding table, the upper end of the first electric push rod is mounted at the lower part of the supporting frame, and the upper end of the second electric push rod is mounted at the lower end of the first electric push rod; the outer side of the upper end of the second electric push rod and the outer side end of the barrel of the liquid suction device are installed together, and the lower end of the second electric push rod and the upper end of a piston rod of the liquid suction device are installed together; the buffer solution tank is mounted on one side of the lower end in the shell of the microplate reader body; the vibration motor is mounted in a mounting groove of the microplate reader body; the control circuit is mounted on the element board. According to the utility model, convenience is brought to detection personnel, and the detection efficiency is correspondingly improved. Based on the above, the system has a good application prospect.

Description

Technical Field

[0001] The utility model relates to the technical field of detection equipment, in particular to an enzyme-labeling instrument capable of automatically adding buffer solution. Background Art

[0002] An enzyme-labeling instrument is a very important medical device with great application value and is widely used in many industries such as major hospitals, university scientific research institutions, disease control centers, technical quality supervision bureaus, animal and plant inspection and quarantine, and the food and feed industries. In the application of the enzyme-labeling instrument, the inspector opens the door of the lower front end of the housing, and then places the liquid sample to be detected (in a plastic microplate) on the internal tray (the upper left, right, and rear parts of the tray are provided with limit plates to position the microplate on the tray to ensure that the subsequent monochromatic light can effectively enter the specimen to be detected in the plastic microplate). After closing the door, the inspector controls the device to detect the sample by operating the buttons on the upper front end of the housing. During the detection, the light wave emitted by the light source lamp at the upper end of the enzyme-labeling instrument passes through a filter or a monochromator to become a beam of monochromatic light, enters the specimen to be detected in the plastic microplate, and then a part of the monochromatic light is absorbed by the specimen, and the other part passes through the specimen and is irradiated onto the photodetector at the lower end of the enzyme-labeling instrument through the transparent tray. The photodetector inputs the light signals of different intensities corresponding to the different specimens to be detected into the signal input end of the signal conversion board of the enzyme-labeling instrument. The conversion board converts it into corresponding electrical signals, and the electrical signals are subjected to signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion and then sent to the microprocessor for data processing and calculation. Finally, the data results of the detected sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen, or printed out through an externally configured printer.

[0003] Before the enzyme-labeling instrument detects a sample, a diluent (also called buffer solution, generally pure water) is used to dilute the sample. Specifically, the inspector puts the test sample into the microplate, then uses a pipette to draw a certain amount of buffer solution into the microplate, and then puts the microplate into an oscillation device or stirs it with a stirring rod to mix the sample and the buffer solution and enter the detection process. The above method is relatively cumbersome in operation because it requires the inspector to manually operate the pipette to draw the buffer solution and drop it into the sample, and also requires mixing the sample and the buffer solution, which will bring inconvenience to the inspector and is not conducive to improving work efficiency. Moreover, due to the different detection skills of the inspectors, when the inspector operates unskilledly, it cannot be guaranteed that the amount of buffer solution drawn by manually operating the pipette meets the requirements. That is to say, when the drawn buffer solution is too much or too little, the sample cannot achieve a good dilution effect, which will correspondingly have a certain impact on the detection effect. In summary, it is particularly necessary to provide an enzyme-labeling instrument that can automatically and quantitatively add buffer solution to the sample in the microplate. Content of the Utility Model

[0004] In order to overcome the drawbacks described in the background art due to the limitations of the structure of existing microplate readers, the present utility model provides a microplate reader capable of automatically adding buffer solution. Based on the microplate reader body, during application, after the sample in the plastic microplate is placed on the detection station of the tray, it can automatically control the pipettor to move to the upper end of the buffer solution tank, quantitatively extract the buffer solution, then drop the buffer solution into the sample in the microplate, and vibrate and mix the sample and the buffer solution, thus bringing convenience to the testers and correspondingly improving the detection efficiency.

[0005] The technical solution adopted by the present utility model to solve its technical problems is as follows:

[0006] A microplate reader capable of automatically adding buffer solution, comprising an electric linear slide, a microplate reader body, a buffer solution tank, an electric push rod, a pipettor, and a vibration motor. It is characterized in that it further has a control circuit; the electric linear slide is installed at the upper rear end inside the housing of the microplate reader body, a support frame is installed at the lower end of the sliding block of the electric linear slide, there are at least two sets of electric push rods, the upper end of the first set of electric push rods is installed at the lower part of the support frame, and the upper end of the second set of electric push rods is installed at the lower end of the first set of electric push rods; a connecting plate is installed outside the upper end of the second set of electric push rods, the lower end of the connecting plate is installed together with the outer side end of the barrel of the pipettor, and the lower end of the second set of electric push rods is installed together with the upper end of the piston rod of the pipettor; the buffer solution tank is installed at the lower end on one side inside the housing of the microplate reader body; there is an installation groove at the lower outer end of the microplate reader body, and the vibration motor is installed in the installation groove; the control circuit is installed on the component board of the microplate reader body, and the power output end of the control circuit is electrically connected to the power input ends of the two sets of electric push rods, the electric linear slide, the vibration motor, and the buffer solution tank respectively.

[0007] Further, the electric linear slide is located at the rear side end of the light source lamp of the microplate reader body.

[0008] Further, the pipettor is a medical syringe.

[0009] Further, the buffer solution tank includes a tank body, a motor reduction mechanism, and a cover plate. The motor reduction mechanism is installed outside one side end of the tank body, its rotating shaft is located at the upper end, one end of the cover plate is installed together with the upper end of the rotating shaft, the outer diameter of the cover plate is larger than the outer diameter of the upper end of the tank body, buffer solution is filled in the tank body, and there is a gap between the lower end of the cover plate and the upper end of the tank body.

[0010] Further, the control circuit includes a single-chip microcomputer module electrically connected to resistors, capacitors, crystal oscillators, triodes, and relays. The reset terminal of the single-chip microcomputer module is connected to one end of a resistor. The external crystal oscillator terminals of the single-chip microcomputer are respectively connected to both ends of the crystal oscillator. The negative power input terminal of the single-chip microcomputer is connected to the other end of the resistor, the negative electrode of the capacitor, the negative power input terminals of nine relays, and the negative control power input terminal. The positive power input terminal of the single-chip microcomputer is connected to the positive electrode of the electrolytic capacitor, the emitters of nine triodes, and the positive control power input terminals of nine relays. The collectors of nine triodes are respectively connected to the positive power input terminals of nine relays. The nine output terminal pins of the single-chip microcomputer are respectively connected to the bases of nine triodes.

[0011] Further, the two normally open contact terminals of the fifth relay and the seventh relay of the control circuit are respectively and electrically connected to both ends of the power input of a time control switch. The power output terminals of the two time control switches are respectively and electrically connected to the power input terminals of the second set of electric push rods.

[0012] The beneficial effects of the present utility model are as follows: Based on the enzyme label instrument body, etc., before detection, the tester places the liquid sample to be detected on the tray and turns on the power switch. The control circuit can control the cover of the buffer tank to open, and then the liquid suction device runs to the upper end of the buffer tank to extract a certain amount of buffer liquid. Then the liquid suction device moves to the microplate to inject the buffer liquid into the sample. Subsequently, the vibration motor vibrates and mixes the buffer liquid and the sample. Since the whole process is fully automated, it brings convenience to the tester and improves the detection efficiency accordingly. Based on the above, the present utility model has a good application prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0014] Figure 1 It is a schematic diagram of the overall structure and partial enlarged structure of the present utility model.

[0015] Figure 2 It is a schematic diagram of the partial enlarged structure of the present utility model.

[0016] Figure 3 It is a circuit diagram of the present utility model. SPECIFIC EMBODIMENTS

[0017] Figure 1 、 2, as shown in FIGS. 2 and 3, an ELISA reader capable of automatically adding buffer includes a power supply module E1, an electric linear slide M1, an ELISA reader body 1, a buffer tank, electric push rods M2 and M3, a liquid aspirator 2, a vibration motor M4, and also has a control circuit 3; the electric linear slide M1 is horizontally distributed, and its upper end of the housing is installed inside the upper rear end of the housing of the ELISA reader body 1 through bolts. A "┌" - shaped support frame 4 is vertically welded to the lower end of the sliding block of the electric linear slide M1. There are two sets of small electric push rods. The upper end of the cylinder of the first set of electric push rod M2 is vertically distributed and installed at the lower front part of the support frame 4 through bolts. The upper end of the cylinder of the second set of electric push rod M3 is vertically distributed and installed at the lower end of the push column of the first set of electric push rod M2 through bolts; on the outer sides of the lower ends of both sides of the cylinder of the second set of electric push rod M3, a connecting plate 5 is respectively welded. The lower ends of the connecting plates 5 and the upper parts of the outer sides of the two cylinders of the liquid aspirator 2 are adhesively bonded together respectively. The lower end of the push column of the second set of electric push rod M3 and the upper end of the piston rod of the liquid aspirator 2 are adhesively bonded together; the lower end of the buffer tank is installed at the lower left middle part inside the housing of the ELISA reader body 1 through bolts; there is an installation groove 101 in the middle of the lower outer end of the ELISA reader body 1, and the vibration motor M4 is installed in the installation groove through bolts; the power supply module E1 and the control circuit 3 are installed on the component board of the ELISA reader body 1.

[0018] Figure 1 , 2As shown in Figures 1 and 3, the housing of the electric linear slide M1 is located at the rear end of the light source lamp inside the ELISA reader body 1. The liquid aspirator 2 is a medical piston syringe. The buffer tank includes a tank body 61 (the height of the lower end is about 2 cm higher than the upper end of the tray), a motor reduction mechanism M5, and a cover plate 62. The motor reduction mechanism M5 is vertically distributed and installed on the middle part of the outer upper part of the left end of the tank body 61 through bolts, and its rotating shaft is located at the upper end. The lower middle part of the left end of the cover plate 62 and the upper end of the rotating shaft are welded together. The outer diameter of the cover plate 62 is larger than the outer diameter of the upper end of the tank body 61. Buffer liquid is filled in the tank body 62. There is a very small gap (0.5 mm) between the lower end of the cover plate 62 and the upper end of the tank body 61. Usually, the cover plate covers the tank body to prevent impurities from entering the tank body. The control circuit includes a single-chip microcomputer module E2, a resistor R, a capacitor C, a crystal oscillator B, a triode, and a relay connected by circuit board wiring. The reset terminal 1 of the single-chip microcomputer module E2 is connected to one end of the resistor R. The external crystal oscillator terminals 4 and 5 of the single-chip microcomputer E2 are respectively connected to both ends of the crystal oscillator B. The negative power input terminal 10 of the single-chip microcomputer E2, the other end of the resistor R, the negative electrode of the capacitor C, the negative power input terminals and the negative control power input terminals of nine relays K1, K2, K3, K4, K5, K6, K7, K8, and K9 are connected. The positive power input terminal 20 of the single-chip microcomputer E2, the positive electrode of the electrolytic capacitor C, the emitters of nine triodes Q1, Q2, Q3, Q4, Q5, Q6, Q7, Q8, and Q9, and the positive control power input terminals of nine relays K1, K2, K3, K4, K5, K6, K7, K8, and K9 are connected. The collectors of nine triodes Q1, Q2, Q3, Q4, Q5, Q6, Q7, Q8, and Q9 are respectively connected to the positive power input terminals of nine relays K1, K2, K3, K4, K5, K6, K7, K8, and K9. The nine output terminals 12, 13, 14, 15, 16, 17, 18, 19, and 11 of the single-chip microcomputer are respectively connected to the bases of nine triodes Q1, Q2, Q3, Q4, Q5, Q6, Q7, Q8, and Q9. The two normally open contact terminals of the fifth relay K5 and the sixth relay K6 of the control circuit are respectively connected to the power input terminals 1 and 2 of a time control switch E3 and E4 through wires. The power output terminals 3 and 4 of the two time control switches E3 and E4 are respectively connected to the negative and positive and positive and negative power input terminals of the second set of electric push rods M3 through wires. The power input terminals 1 and 2 of the power module E1 are respectively connected to the two poles of the AC 220V power supply through wires. The power output terminals 3 and 4 of the power module E1 are connected in series with a power switch S1 and the power input terminals 1 and 2 of the single-chip microcomputer module E2, which is the power input terminal of the control circuit, through wires.The two normally open contact terminals of relays K1 and K2 and the positive, negative, negative, and positive power input terminals of the electric linear slide M1 are respectively connected by wires. The two normally open contact terminals of relays K3 and K4 and the positive, negative, negative, and positive power input terminals of the first set of electric push rods M2 are respectively connected by wires. The two normally open contact terminals of relays K7 and K8 and the positive, negative, negative, and positive power input terminals of the motor reduction mechanism M5 are respectively connected by wires. The two normally open contact terminals of relay K9 and the two power input ends of the vibration motor M4 are respectively connected by wires.

[0019] Figure 1 、 2 As shown in FIGS. 2 and 3, the present invention is based on the enzyme immunoassay analyzer body 1, and other detection methods are exactly the same as those of the existing enzyme immunoassay analyzer. During the application of the enzyme immunoassay analyzer body 1, the operator opens the door at the lower front end of the housing, and then places the sample to be detected (located in a plastic microplate) on the internal tray (the upper left, upper right, and rear parts of the tray are provided with limit plates to position the microplate on the tray, ensuring that the subsequent monochromatic light can effectively enter the specimen to be detected in the plastic microplate). After closing the door, the operator controls the device to detect the sample by operating the buttons at the upper front end of the housing of the enzyme immunoassay analyzer body 1. During the detection, the light wave emitted by the light source lamp at the upper end of the enzyme immunoassay analyzer passes through the filter or monochromator to become a beam of monochromatic light, which enters the specimen to be detected in the plastic microplate. Then, a part of the monochromatic light is absorbed by the specimen, and the other part passes through the specimen and is irradiated onto the photodetector at the lower end of the enzyme immunoassay analyzer through the transparent tray. The photodetector inputs the light signals of different intensities corresponding to the specimen to be detected into the signal input end of the conversion board of the enzyme immunoassay analyzer. The conversion board converts it into corresponding electrical signals, and the electrical signals are subjected to signal processing such as pre-amplification, logarithmic amplification, and analog-to-digital conversion and then sent to the microprocessor for data processing and calculation. Finally, the data results of the detected sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen, or printed out through an externally equipped printer. The above is the existing mature technology, and the present application will not elaborate on its working principle nor claim any protection for the above technical solution.

[0020] Figure 1 、 2 As shown in FIGS. 2 and 3, in the present application, after the 220V AC power supply enters the two power input ends 1 and 2 of the power module E1, the output terminals 3 and 4 of the power module E1 output a stable 12V DC power supply. In the present application, after the operator places the sample to be detected on the internal tray and closes the door, then turns on the power switch S1, the 12V DC power supply enters the power input end of the control circuit, and thus, the control circuit is in a powered-on state.

[0021] After the control circuit is powered on and working, the single-chip microcomputer module E2, under the combined action of its internal circuit and peripheral components, namely crystal oscillator B (the crystal oscillator serves as the clock source, providing time reference and timing control signals for the single-chip microcomputer module), electrolytic capacitor C (for filtering), and resistor R (for reset, mainly to change the module power supply voltage of the single-chip microcomputer module from high level to low level, so that the single-chip microcomputer restarts), first outputs a low level for 5 seconds from its pin 12 to the base of transistor Q1. Then, transistor Q1 amplifies the low-level power and inverts it to the positive power input terminal of relay K1. Relay K1 is powered on and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the electric linear slide M1 are powered on, and its slider drives the liquid suction device 2 to move to the middle upper part of the tank body 61 and stop. At the same time as the pin 12 of the single-chip microcomputer module E2 outputs a low level, its pin 18 synchronously outputs a low level for 3 seconds to the base of transistor Q7. Then, transistor Q7 amplifies the low-level power and inverts it to the positive power input terminal of relay K7. Relay K7 is powered on and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the motor reduction mechanism M5 are powered on, and its rotating shaft drives the cover plate 62 to rotate 180 degrees clockwise and stop. The cover plate no longer blocks the upper end of the tank body 61. One second after the pin 12 of the single-chip microcomputer module E2 outputs a low level, its pin 14 outputs a low level for 3 seconds to the base of transistor Q3. Then, transistor Q3 amplifies the low-level power and inverts it to the positive power input terminal of relay K3. Relay K3 is powered on and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the electric push rod M2 are powered on, and its push rod drives the liquid suction device 2 to descend to a certain height and stop with its lower end located inside the tank body 61 (the lower end of the liquid inlet pipe of the syringe is about 3 mm away from the lower end inside the tank). One second after the pin 14 of the single-chip microcomputer module E2 outputs a low level, its pin 16 outputs a low level for 5 seconds to the base of transistor Q5. Then, transistor Q5 amplifies the low-level power and inverts it to the positive power input terminal of relay K5. Relay K5 is powered on and its control power input terminal and normally open contact terminal are closed. In this way, the time control switch E3 is powered on and works for 5 seconds. After the time control switch E3 is powered on, its pins 3 and 4 output power for a certain period of time (adjustable, such as 2 seconds) to the negative and positive power input terminals of the electric push rod M3. In this way, the push rod of the electric push rod M3 drives the piston rod of the syringe (liquid suction device) to move upward a certain distance and stop. Then, the syringe quantitatively sucks a part of the buffer liquid in the tank into the lower end of its barrel (the longer the syringe works, the more liquid is quantitatively sucked in, and vice versa).

[0022] Figure 1 , 2As shown in Figures 3, after the 16th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 15th pin outputs a low level for 3 seconds and enters the base of the triode Q4. Then, the triode Q4 amplifies the low-level power and inverts the input to the positive power input terminal of the relay K4. The relay K4 is energized and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the electric push rod M2 are energized, and its push rod drives the liquid absorber 2 to rise in height. The lower end of the liquid absorber 2 stops moving outside the upper part of the tank body 61. After the 15th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 13th pin outputs a low level for 5 seconds and enters the base of the triode Q2. Then, the triode Q2 amplifies the low-level power and inverts the input to the positive power input terminal of the relay K2. The relay K2 is energized and its control power input terminal and normally open contact terminal are closed. The positive and negative power input terminals of the electric linear slide M1 are energized, and its slider drives the liquid absorber 2 to move to the middle-upper position of the plastic microplate and stop moving. While the 13th pin of the single-chip microcomputer module E2 outputs a low level, its 19th pin synchronously outputs a low level for 3 seconds and enters the base of the triode Q8. Then, the triode Q8 amplifies the low-level power and inverts the input to the positive power input terminal of the relay K8. The relay K8 is energized and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the motor reduction mechanism M5 are energized, and its rotating shaft drives the cover plate 62 to rotate counterclockwise by about 180 degrees and stop moving. The cover plate completely covers the upper end of the tank body 61 to prevent impurities from entering the tank body. After the 13th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 14th pin outputs a low level for 3 seconds again and enters the base of the triode Q3. Then, the triode Q3 amplifies the low-level power and inverts the input to the positive power input terminal of the relay K3. The relay K3 is energized and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the electric push rod M2 are energized, and its push rod drives the liquid absorber 2 to descend in height. Its lower end is located in the middle-upper part of the plastic microplate and stops moving at a close interval (the lower end of the syringe is about 2 mm in the middle-upper part of the plastic microplate). After the 14th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 17th pin outputs a low level for 5 seconds and enters the base of the triode Q6. Then, the triode Q6 amplifies the low-level power and inverts the input to the positive power input terminal of the relay K6. The relay K6 is energized and its control power input terminal and normally open contact terminal are closed. In this way, the time control switch E4 is energized and works for 5 seconds. After the time control switch E4 is energized, its pins 3 and 4 output power for a certain period of time (the time is adjustable, such as 2 seconds) and enter the positive and negative power input terminals of the electric push rod M3. In this way, the push rod of the electric push rod M3 drives the piston rod of the syringe (liquid absorber) to move downward (the piston moves downward) for a certain distance and stop moving. Then, the syringe discharges the pre-quantitatively drawn buffer solution into the liquid in the middle-upper part of the plastic microplate.After the 17th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 15th pin outputs a low level for another 3 seconds and enters the base of the triode Q4. Then, the triode Q4 amplifies the low-level power and inverts it to input to the positive power input terminal of the relay K4. The relay K4 is energized and its control power input terminal and normally open contact terminal are closed. In this way, the positive and negative power input terminals of the electric push rod M2 are energized, and its push rod drives the liquid absorber 2 to rise. The liquid absorber 2 stops moving at the middle upper position of the plastic microplate on the tank body 61 (the height of the lower end of the syringe is higher than the height of the upper end of the plastic microplate), preparing for the next detection of pipetting the liquid into the plastic microplate. After the 15th pin of the single-chip microcomputer module E2 outputs a low level and intervals for 1 second, its 11th pin outputs a low level for 6 seconds and enters the base of the triode Q9. Then, the triode Q9 amplifies the low-level power and inverts it to input to the positive power input terminal of the relay K9. The relay K9 is energized and its control power input terminal and normally open contact terminal are closed. The vibration motor M5 is energized to generate vibration to vibrate and mix the sample and the buffer solution. After the subsequent detection is completed, the staff turns off the power switch, opens the chamber door, and takes out the detected sample. The process is exactly the same as the above, and the detection of the next sample can be entered again.

[0023] Figure 3 As shown, the power module E1 is a finished product of an AC 220V to DC 12V switching module; the model of the single-chip microcomputer module E2 is AT89C2051-24P, the resistance value of the resistor R is 1K, the specification of the crystal oscillator B is 11.0592MHz, the specification of the electrolytic capacitor C is 10μF / 25V, and the models of the relays K1, K2, K3, K4, K5, K6, K7, K8, K9 are 4123 / DC12V. The models of the PNP triodes Q1, Q2, Q3, Q4, Q5, Q6, Q7, Q8, Q9 are 9012; the electric push rods M2 and M3 are finished products of small reciprocating electric telescopic rods (power 5W); the electric linear slide table M1 is a finished product of an electric screw slide table with a power of 20W; the motor reduction mechanism M5 is a finished product of a coaxial motor gear reducer (power 10W); the time control switches E4 and E3 are finished products of the microcomputer time control switch of model KG316T, which has two power input terminals, two power output terminals, and seven setting buttons. By operating the seven buttons respectively, the time for the two power output terminals to output power can be set; the power of the vibration motor M4 is 50W. It should be noted that it is an extremely mature technology that the single-chip microcomputer module and its peripheral components work together to control the output of level signals at intervals (including cyclic output) at its multiple power output terminals. This application does not claim any protection for the single-chip microcomputer module and its peripheral components working together to control the output of level signals at intervals at its multiple power output terminals. What this application protects is the technical solution that the single-chip microcomputer module can control relevant components to quantitatively add buffer solution to the detection samples of the enzyme label instrument.

[0024] The foregoing has shown and described the basic principles, main features and advantages of the present utility model. For those skilled in the art, it is obvious that the present utility model is limited to the details of the above-mentioned exemplary embodiments, and without departing from the spirit or basic features of the present utility model, the present utility model can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes that fall within the meaning and scope of the equivalent elements of the claims in the present utility model. Any reference signs in the claims should not be construed as limiting the claimed claim.

[0025] In addition, it should be understood that although this specification is described according to the embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An ELISA reader capable of automatically adding buffer, comprising an electric linear slide table, an ELISA reader body, a buffer tank, an electric push rod, a liquid aspirator, and a vibration motor, characterized in that, It also has a control circuit; the electric linear slide is installed at the upper rear end inside the housing of the ELISA reader body, a support frame is installed at the lower end of the sliding block of the electric linear slide, there are at least two sets of electric push rods, the upper end of the first set of electric push rods is installed at the lower part of the support frame, and the upper end of the second set of electric push rods is installed at the lower end of the first set of electric push rods; a connecting plate is installed outside the upper end of the second set of electric push rods, the lower end of the connecting plate is installed together with the outer side end of the cylinder body of the liquid absorber, and the lower end of the second set of electric push rods is installed together with the upper end of the piston rod of the liquid absorber; the buffer solution tank is installed at the lower end side inside the housing of the ELISA reader body; there is an installation groove at the lower outer end of the ELISA reader body, and the vibration motor is installed in the installation groove; the control circuit is installed on the component board of the ELISA reader body, and the power output end of the control circuit is electrically connected to the power input ends of the two sets of electric push rods, the electric linear slide, the vibration motor, and the buffer solution tank respectively.

2. The microplate reader capable of automatically adding buffer according to claim 1, characterized in that, The electric linear slide is located at the rear side end of the light source lamp of the ELISA reader body.

3. The microplate reader capable of automatically adding buffer solution according to claim 1, characterized in that, The liquid absorber is a medical syringe.

4. An ELISA reader capable of automatically adding buffer according to claim 1, characterized in that, The buffer solution tank includes a tank body, a motor reduction mechanism, and a cover plate. The motor reduction mechanism is installed outside one side end of the tank body, its rotating shaft is located at the upper end, one end of the cover plate is installed together with the upper end of the rotating shaft, the outer diameter of the cover plate is larger than the outer diameter of the upper end of the tank body, buffer solution is filled in the tank body, and there is a spacing between the lower end of the cover plate and the upper end of the tank body.

5. An ELISA reader capable of automatically adding buffer according to claim 1, characterized in that, The control circuit includes a single-chip microcomputer module electrically connected to resistors, capacitors, crystal oscillators, triodes, and relays. The reset end of the single-chip microcomputer module is connected to one end of the resistor, the external crystal oscillator terminals of the single-chip microcomputer are respectively connected to both ends of the crystal oscillator, the negative power input terminal of the single-chip microcomputer is connected to the other end of the resistor, the negative electrode of the capacitor, the negative power input terminals and the negative control power input terminals of nine relays, and the positive power input terminal of the single-chip microcomputer is connected to the positive electrode of the electrolytic capacitor, the emitters of nine triodes, and the positive control power input terminals of nine relays. The collectors of nine triodes are respectively connected to the positive power input terminals of nine relays, and the nine output terminal pins of the single-chip microcomputer are respectively connected to the bases of nine triodes.

6. The microplate reader capable of automatically adding buffer according to claim 5, characterized in that, The two normally open contact terminals of the fifth relay and the seventh relay of the control circuit are respectively electrically connected to both ends of the power input of a time control switch, and the power output ends of the two time control switches are respectively electrically connected to the power input end of the second set of electric push rods.

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