Thermophilic bacteria spore culture device
By setting up a water supply area and a culture area in the thermophilic spore culture device, and using vents and oxygen release boxes, the problem of high-temperature water evaporation is solved, humidity maintenance and oxygen supply are achieved, rust and short circuit in the incubator are avoided, and the amount of culture medium is saved.
Patent Information
- Application Number
- CN202422156574.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-04
- Publication Date
- 2025-07-18
- Estimated Expiration
- 2034-09-04
AI Technical Summary
During the culture of thermophilic spores, high-temperature moisture evaporation affects the test effect and can easily cause incubator rust and circuit short circuit.
A thermophilic bacteria spore culture device is designed, including a box and a box lid. The box body is equipped with a water supply area and a culture area. The water supply area is used to place a water box or add water. The culture area is used to place a culture plate. The evaporated water vapor is transported to the culture area through the ventilation hole to maintain humidity, and an oxygen release box is installed in the box lid to supply oxygen.
Effectively prevent water vapor from gathering on the inner wall of the incubator, avoid rust and short circuits, save the amount of culture medium, and reduce costs.
Smart Images
Figure CN223118427U_ABST
Abstract
Description
Technical Field
[0001] The utility model provides a thermophilic bacteria spore culture device, which relates to the technical field of microbial culture devices. Background Art
[0002] The spores of Bacillus stearothermophilus have a relatively special cell structure, which is more heat-resistant than the spores of general bacteria. Therefore, they are used to prepare high-pressure steam sterilization indicators to evaluate whether the expected sterilization effect is achieved. The spores of Bacillus stearothermophilus need to be cultured at 56°C for 5-7 days. Due to the high culture temperature, in order to avoid cracking and shrinking of the petri dishes during the culture process, glass petri dishes are used, and as much culture medium as possible, about 50 ml / plate, is poured into each petri dish. During the culture process, a water tray is placed in the incubator to keep water in the water tray to maintain the environmental humidity and reduce the evaporation of water in the culture dish. If water is not added in time, the test effect will be affected. The culture temperature is 56°C, which causes a large amount of water to evaporate from the culture medium. About 2 L of water needs to be added every two days. The evaporated water is easy to accumulate on the inner wall of the incubator and other places, which is easy to cause rust of the incubator and even short circuit of the circuit. It can be seen that adding water has a great potential safety hazard. Content of the Utility Model
[0003] The utility model provides a thermophilic bacteria spore culture device, which solves the technical problems in the prior art that the test effect is easily affected by high-temperature water evaporation during the culture process of thermophilic bacteria spores, and the evaporated water vapor is easy to cause rust of the incubator and even short circuit of the circuit.
[0004] The utility model is realized as follows: it includes a box cover and a box body. The box body includes side plates, several baffles and a bottom plate. All the baffles are vertically installed on the bottom plate, and several ventilation holes are provided on the upper parts of all the baffles. The baffles divide the inside of the box body into at least three areas. The area in the middle of the box body is the water supply area, the areas around the water supply area are the culture areas, and the side plates within the range of the water supply area are provided with water replenishing holes and rubber stoppers. The side of the box cover facing the box body is provided with an oxygen release box. The oxygen release box is located above the water supply area. The oxygen release box is provided with an oxygen addition box cover, and the oxygen addition box cover is installed on the box cover. Oxygen release holes are provided on the side of the oxygen release box.
[0005] The water supply area is used to place a water box or directly add water to the water supply area. The culture areas are used to place culture plates. The water supply area is located in the middle of the box body, and the culture areas are around the water supply area to ensure uniform delivery of water vapor to the surrounding areas. Water is added to the water box through the water replenishing holes to prevent too much water from being added at one time during the initial culture, which is easy to cause spillage during the handling process.
[0006] As a preferred embodiment, the oxygen addition box cover is provided with several oxygen addition holes.
[0007] As a preferred embodiment, several fixed bases are provided at the bottom of the oxygen release box corresponding to the positions of the oxygen addition holes.
[0008] As a preferred embodiment, oxygen addition holes are provided at the positions of the oxygen addition box cover corresponding to the fixed bases.
[0009] As a preferred embodiment, moisture-proof protrusions are provided on the surface of the bottom plate within the range of the culture area.
[0010] The oxygen release box is generally rectangular, located above the water supply area, and the extension direction of the oxygen release box is the same as that of the water supply area, without occupying the space of the culture area.
[0011] As a preferred embodiment, anti-slip strips are provided on the side of the baffle facing the culture area.
[0012] As a preferred embodiment, support feet are installed at the positions near the four corners of the bottom surface of the bottom plate.
[0013] The support feet facilitate the handling of the culture device.
[0014] As a preferred embodiment, support grooves corresponding to the support feet are installed at the positions near the four corners of the top surface of the box cover.
[0015] The anti-slip strips correspond to the support grooves, which is beneficial for stacking multiple thermophilic bacteria spore culture devices together.
[0016] As a preferred embodiment, limit protrusions are installed on the bottom plate within the range of the culture area.
[0017] The anti-slip strips further limit the position of the culture plate and prevent the culture plate from sliding.
[0018] As a preferred embodiment, buckle seats are installed along the upper edge of the side plate, and buckles are installed at the corresponding positions of the box cover and the buckle seats.
[0019] As a preferred embodiment, the height of the baffle is less than the height of the side plate.
[0020] The working principle of the present utility model is that the box body is provided with two types of areas, one for holding water and the other for culturing plates. During use, stack the inoculated plates in the culture area of the box body, inject an appropriate amount of water into the water supply area; cover the box cover; open the oxygen addition box cover on the box cover, put in the oxygen generating agent (pour the liquid directly into the cavity, or use a finished ampoule bottle containing the oxygen generating agent and insert it on the ampoule bottle seat in the oxygen release cavity), and cover the oxygen addition box cover; conduct the culture. If water needs to be replenished, add it through the water replenishment hole, and if the oxygen generating agent needs to be replenished, add it through the oxygen addition hole.
[0021] In the present utility model, a water supply area is added inside the culture device. The water supply area is used to place a water box (here, water can be directly poured into the water supply area, or a special water-containing sleeve box can be added for convenient removal and cleaning). The evaporated water enters the culture area through the ventilation holes on the baffle to maintain the humidity in the culture area. The water supply area is provided with a water replenishing hole, and when adding water, there is no need to open the box cover. Instead, water is added to the water supply area through the water replenishing hole. The culture device is not connected to the outside world, so the water vapor formed by evaporation will not enter the incubator, thus avoiding the accumulation of evaporated water on the inner wall of the incubator and other places, and preventing the rust and even short circuit of the incubator. When using the culture device of the present utility model, each plate only needs to be added with the normal amount of culture medium, which is beneficial to cost savings.
[0022] An oxygen release box is installed on the inner surface of the box cover. The oxygen release box is used to place a liquid that can release oxygen or an oxygen generator in an ampoule bottle. The oxygen release box is provided with a fixed base adapted to the ampoule bottle for placing the ampoule bottle. The oxygen release box is provided with oxygen release holes, which are beneficial to the diffusion of oxygen to the culture area. The box cover is provided with an oxygen addition cover for the oxygen release box. When oxygen addition is required, the oxygen addition cover is opened to complete oxygen addition.
[0023] A limiting protrusion is provided on the side of the baffle facing the culture area to limit the position of the culture plate. A moisture-proof protrusion is provided at the bottom of the culture area to lift the culture plate and prevent the evaporated water from accumulating at the bottom of the culture area and wetting the culture plate. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0025] Figure 1 is a schematic diagram of the overall structure of an embodiment of the present utility model;
[0026] Figure 2 is Figure 1 a schematic diagram of the box cover structure;
[0027] Figure 3 is Figure 1 a top view schematic diagram of the box body;
[0028] Figure 4 is Figure 1 a schematic diagram of the baffle structure.
[0029] Identifications in the figure: 1 - box cover; 2 - box body; 3 - side plate; 4 - bottom plate; 5 - baffle; 6 - water supply area; 7 - culture area; 8 - ventilation hole; 9 - water replenishing hole; 10 - moisture-proof protrusion; 11 - oxygen release box; 12 - oxygen addition box cover; 13 - oxygen release hole; 14 - fixed base; 15 - oxygen addition hole; 16 - limit protrusion; 17 - anti-slip strip; 18 - support foot; 19 - buckle seat; 20 - buckle. Detailed implementation manners
[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention. Embodiment
[0031] Refer to Figure 1 , Figure 2 , Figure 3 and Figure 4 , a thermophilic bacteria spore culture device, including a box cover 1 and a box body 2, and the box cover 1 is buckled on the box body 2. The box body 2 includes side plates 3, several baffles 5 and a bottom plate 4. The number of the side plates 3 is four, and the four side plates 3 are respectively installed on four sides of the bottom plate 4 to form the square box body 2. In this embodiment, the box body is rectangular as a whole. All the baffles 5 are vertically installed on the bottom plate 4. The baffle 5 and the bottom plate 4 may be integrally formed, and the baffle 5 is parallel to the side plate 3 with a longer length. The baffle 5 divides the inside of the box body 2 into at least three areas. The area in the middle of the box body 2 is the water supply area 6, and the areas around the water supply area 6 are the culture areas 7. The water supply area 6 is used to place a water box or directly add water, and the culture area 7 is used to place a culture plate. Several ventilation holes 8 are provided at the upper parts of all the baffles 5. The arrangement mode of the ventilation holes 8 can adopt existing methods, such as evenly distributed at equal intervals, arranged in a circular shape, a square shape, etc. The water supply area 6 is located in the middle of the box body 2, and the culture area 7 is located around the water supply area 6 to ensure that the water vapor evaporated from the water supply area 6 is evenly delivered to the surrounding culture areas 7 through the ventilation holes 8. When the number of the baffles 5 is two, the inside of the box body 2 is divided into three areas. The water supply area 6 extends along the long side of the box body 2 and is rectangular as a whole. At this time, the water storage capacity of the water supply area 6 is the largest, and the effect of one water supply area 6 providing water vapor to the two adjacent culture areas 7 is the best. Refer to Figure 4 , the setting form of the ventilation hole is not limited. The ventilation hole can be circular, square, diamond-shaped or other shapes that can ventilate, as long as it is convenient for water vapor to pass through.
[0032] Refer to Figure 1 , on the side plate 3 within the range of the water supply area 6, there are water replenishing holes 9 and rubber stoppers (not shown in the figure). Water is added into the water supply area 6 through the water replenishing holes 9. The rubber stoppers are adapted to the water replenishing holes 9. The rubber stoppers can be independent or connected to the box body 2, and the connection method is selected from the prior art. By providing the water replenishing holes 9 and the rubber stoppers, it is prevented that when the box cover 1 is opened, a large amount of water evaporates. Only when water needs to be added, water is added into the water supply area 6 through the water replenishing holes 9. An air vent hole 8 is provided at the upper part of the water supply area 6, and the lower part of the water supply area 6 is closed. Therefore, the water supply area 6 can be directly used as a water box, or a special water-containing sleeve box can be added for convenient removal and cleaning. The upper edge of the sleeve box does not exceed the horizontal plane where the bottom of the air vent hole 8 is located, and the water level during water addition does not exceed the horizontal plane where the bottom of the air vent hole 8 is located. On the surface of the bottom plate 4 within the range of the culture area 7, moisture-proof protrusions 10 are provided to prevent moisture from accumulating at the bottom of the culture area 7 and wetting the culture plate. The moisture-proof protrusions 10 can be rivets or protrusions of other shapes.
[0033] Refer to Figure 2 , on the side of the box cover 1 facing the box body 2, there is an oxygen release box 11. When the box cover 1 is buckled on the box body 2, the oxygen release box 11 is located inside the box body 2, more precisely, it falls inside the box body above the water addition area. To facilitate adding substances that release oxygen into the oxygen release box 11, an oxygen addition box cover 12 is provided at the corresponding position of the box cover 1 and the oxygen release box 11. The oxygen addition box cover 12 is installed on the box cover 1, and the installation method is selected from the prior art. The oxygen addition box cover 12 can be buckled on the oxygen release box 11. To facilitate opening the oxygen release box 11, a handle is installed on the oxygen addition box cover 12. Oxygen release holes 13 are provided on the side of the oxygen release box 11 to facilitate the diffusion of oxygen to the surrounding. When placing the ampoule bottle, several fixed bases 14 are provided inside the oxygen release box 13 for fixing the ampoule bottle. To facilitate oxygen addition, oxygen addition holes 15 are provided at the positions of the oxygen addition box cover 12 corresponding to the fixed bases 14. Through the oxygen addition holes 15, a liquid oxygen generating agent is added into the ampoule bottle. It is also possible not to install the fixed bases 14 and just provide oxygen addition holes 15 on the oxygen release box 11, and release oxygen substances are directly added into the oxygen release box 11 through the oxygen addition holes 15.
[0034] Refer to Figure 2 , the oxygen release box 11 is located above the water supply area 6. The extending direction of the oxygen release box 11 is the same as the extending direction of the water supply area 6, and the two are located in the same vertical space without occupying the space of the culture area 7.
[0035] Refer to Figure 1 and Figure 3, on the side of the baffle 5 facing the culture area, there is an anti-slip strip 17 to help fix the culture dish / culture plate and prevent it from slipping. On the bottom plate 4 within the range of the culture area 7, there are limit protrusions 16 corresponding to the culture dishes installed. The cooperation of the limit protrusions 16 and the anti-slip strip 17 can prevent large sliding displacements of the whole stack of petri dishes and prevent them from scattering. At the positions near the four corners of the bottom surface of the bottom plate 4, support feet 18 are installed. At the positions near the four corners of the top surface of the box cover 1, support grooves (not shown in the figure) corresponding to the support feet 18 are installed. On the upper edge of the side plate 3, a snap seat 19 is installed, and at the corresponding position of the box cover 1, a snap 20 is installed. In order to increase the sealing performance of the culture device, a sealing strip (not shown in the figure) is installed on the inner edge of the box cover 1, and the installation method of the sealing strip adopts the existing technology.
[0036] In the culture device of the present utility model, a water supply area is added. The water supply area is used to place a water box or can directly hold water. The evaporated water enters the culture area through the ventilation holes on the baffle to maintain the humidity of the culture area. The water supply area is provided with a water replenishing hole, so that when adding water, there is no need to open the box cover, but water is added to the water supply area through the water replenishing hole. The culture device is not communicated with the outside world, and the water vapor formed by evaporation will not enter the incubator, thus avoiding the accumulation of evaporated water on the inner wall of the incubator and the like, and preventing the corrosion and even short circuit of the incubator. When using the culture device of the present utility model, each plate only needs to be added with the normal amount of culture medium, which is beneficial to cost saving.
[0037] An oxygen release box is installed on the inner surface of the box cover. The oxygen release box is used to place an oxygen generator that can release oxygen, or an ampoule filled with an oxygen generator. The oxygen generator can be made into a solution or semi-solid form using hydrogen peroxide or calcium peroxide. The oxygen release box is provided with a fixing base adapted to the ampoule for placing the ampoule. The oxygen release box is provided with oxygen release holes to facilitate the diffusion of oxygen to the culture area. The box cover is provided with an oxygen addition cover for the oxygen release box. When oxygen needs to be added, pull up the handle on the oxygen addition cover to open the oxygen addition cover to complete the oxygen addition.
[0038] The working principle of the present utility model is that the box body is provided with two types of areas, one for holding water and the other for culturing plates. When in use, stack the inoculated plates in the culture area of the box body, inject an appropriate amount of water into the water supply area; cover the box cover; open the oxygen addition box cover on the box cover, put in the oxygen generator (pour the liquid directly into the cavity, or directly use a finished ampoule filled with an oxygen generator, and the ampoule can be inserted into the ampoule seat in the oxygen release cavity), and cover the oxygen addition box cover; then carry out the culture. If water needs to be replenished, add it through the water replenishing hole, and if the oxygen generator needs to be replenished, add it through the oxygen addition hole.
[0039] In the present utility model, a water supply area is added inside the culture device. The water supply area is used to place a water tray. The evaporated water enters the culture area through the ventilation holes on the baffle to maintain the humidity of the culture area. The water supply area is provided with a water replenishing hole. When adding water, there is no need to open the box cover, but water is added to the water supply area through the water replenishing hole. The culture device is not connected to the outside world, and the water vapor formed by evaporation will not enter the incubator, thereby avoiding the accumulation of the evaporated water on the inner wall of the incubator and the like, and preventing the rust and even short circuit of the incubator. When using the culture device of the present utility model, only the normal amount of culture medium needs to be added to each plate, which is beneficial to cost saving.
[0040] An oxygen release box is installed on the inner surface of the box cover. The oxygen release box is used to place a liquid that can release oxygen or an oxygen generator in an ampoule bottle. A fixed base adapted to the ampoule bottle is provided inside the oxygen release box for placing the ampoule bottle. The oxygen release box is provided with an oxygen release hole to facilitate the diffusion of oxygen to the culture area. The box cover is provided with an oxygen addition cover for the oxygen release box. When oxygen needs to be added, the oxygen addition cover is opened to complete the oxygen addition. A limiting protrusion is provided on the side of the baffle facing the culture area to limit the position of the culture plate. A moisture-proof protrusion is provided at the bottom of the culture area to lift the culture plate and prevent the evaporated water from accumulating at the bottom of the culture area and wetting the culture plate.
[0041] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included in the protection scope of the present utility model.
Claims
1. Thermophilic spore culture device, characterized in that, It includes a box cover and a box body. The box body includes side plates, several baffles and a bottom plate. The four side plates are respectively installed on the four sides of the bottom plate. All the baffles are vertically installed on the bottom plate. Several ventilation holes are provided at the upper parts of all the baffles. The baffles divide the interior of the box body into at least three areas. The area in the middle of the box body is the water supply area. The areas around the water supply area are the cultivation areas. Water replenishing holes are provided on the side plates within the range of the water supply area. Rubber stoppers are installed in the water replenishing holes. On the side of the box cover facing the box body, there is an oxygen release box. The oxygen release box is located above the water supply area. The oxygen release box is provided with an oxygen adding box cover. The oxygen adding box cover is installed on the box cover. Oxygen release holes are provided on the side surface of the oxygen release box.
2. The thermophilic spore culture device according to claim 1, characterized in that, The oxygen adding box cover is provided with several oxygen adding holes.
3. The thermophilic spore culture device according to claim 2, characterized in that, Several fixed bases are provided at the bottom of the oxygen release box corresponding to the positions of the oxygen adding holes.
4. The thermophilic spore culture device according to claim 1, characterized in that Moisture-proof protrusions are provided on the surface of the bottom plate within the range of the cultivation area.
5. The thermophilic spore culture device according to claim 1, characterized in that, Anti-slip strips are provided on the side of the baffle facing the cultivation area.
6. The thermophilic spore culture device according to claim 1, characterized in that, Support feet are installed at the positions near the four corners on the bottom surface of the bottom plate.
7. The thermophilic spore culture device according to claim 6, characterized in that, Support grooves corresponding to the support feet are installed at the positions near the four corners on the top surface of the box cover.
8. The thermophilic spore culture device according to claim 1, characterized in that, Limit protrusions are installed on the bottom plate within the range of the cultivation area.
9. The thermophilic spore culture device according to claim 1, wherein Snap seats are installed along the upper edge of the side plate, and snaps are installed at the corresponding positions on the box cover.
10. The thermophilic spore culture device according to claim 1, characterized in that, The height of the baffle is less than the height of the side plate.