Cell culture plate sample adding positioning device

Abstract

The utility model discloses a cell culture plate sample adding and positioning device which comprises a culture plate body, a supporting frame is fixedly arranged on the surface of the culture plate body, a mounting frame is arranged on the supporting frame through a fixing assembly, a connecting rod is fixedly arranged in the mounting frame, an auxiliary plate is arranged on the surface of the connecting rod in a sliding mode, and the auxiliary plate is arranged on the surface of the connecting rod. An auxiliary frame is fixedly arranged at the top of the auxiliary plate, a supporting shaft is rotatably arranged in the auxiliary frame, an elastic piece is arranged on the surface of the supporting shaft, shielding cloth is wound on the surface of the supporting shaft, one end of the shielding cloth is fixedly connected with the supporting shaft, and a moving plate is fixedly arranged at the other end of the shielding cloth; according to the device, an operator can be reminded to add samples into the culture holes in the same vertical column through the auxiliary plate, and meanwhile, the culture holes in the same vertical column can be shielded after sample adding is completed, so that the conditions of missing sample adding and repeated sample adding during cell culture are avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a sample adding and positioning device for a cell culture plate. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to allow cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture technology is an important and commonly used technology in cell biology research methods. Cell culture can not only obtain a large number of cells, but also study cell signal transduction, cell anabolism, cell growth and proliferation, etc.

[0003] Cell culture plates are needed for cell culture. Cell culture plates are used to culture cells or measure protein concentration. Cell culture plates are divided into flat bottom and round bottom according to the different shapes of the bottom. The number of culture wells is 6, 12, 24, 48, 96, 384, 1536, etc. Among them, 96-well cell culture plates are one of the commonly used specifications.

[0004] In the actual use of 96-well cell culture plates, the gaps between adjacent culture wells are small and the arrangement is relatively dense. At the same time, there are no labels between the culture wells to assist operators in culture the wells separately. During the cell culture process, operators are often required to add samples to multiple culture wells. Therefore, when the culture plates are not labeled, repeated additions and missed additions are prone to occur, thus affecting the experimental data. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a cell culture plate sample loading and positioning device, which is used to solve the problems raised in the above-mentioned background technology.

[0006] The above-mentioned technical purpose of the utility model is achieved through the following technical solutions: a cell culture plate loading and positioning device, comprising a culture plate body, a support frame fixedly arranged on the surface of the culture plate body, a fixing component arranged inside the support frame, a mounting frame arranged on the support frame through the fixing component, an identification plate fixedly arranged inside the mounting frame, a connecting rod fixedly arranged inside the mounting frame, an auxiliary plate slidably arranged on the surface of the connecting rod, an auxiliary frame fixedly arranged on the top of the auxiliary plate, a support shaft rotatably arranged inside the auxiliary frame, an elastic part arranged on the surface of the support shaft, a shielding cloth wound on the surface of the support shaft, one end of the shielding cloth is fixedly connected to the support shaft, a movable plate is fixedly arranged on the other end of the shielding cloth, and a loading hole is opened on the surface of the movable plate.

[0007] Preferably, the fixing component includes a threaded groove formed on the surface of the support frame, a bolt is threadedly arranged inside the threaded groove, and the bolt is threadedly connected to the mounting frame.

[0008] Preferably, a marking frame is fixedly arranged on the top of the marking board, and the number of the marking frames is multiple.

[0009] Preferably, the elastic member is a coil spring, one end of the elastic member is fixedly connected to the surface of the support shaft, and the other end of the elastic member is fixedly connected to the auxiliary frame.

[0010] Preferably, a knob is fixedly arranged on the top of the bolt, and the knob is made of rubber.

[0011] Preferably, the number of the connecting rods is two, and the two connecting rods are symmetrically distributed on the front and rear sides of the mounting frame.

[0012] Compared with the prior art, the beneficial effect of the present utility model is as follows: for this cell culture plate sample addition positioning device, the auxiliary plate and the moving plate are moved to the specified vertical column to be sampled, then the sample addition port of the sample addition device is placed inside the sample addition hole, and then the sample addition port of the sample addition device adds the reagent into the culture hole at the bottom of the sample addition hole. Subsequently, the moving plate is pulled to move, so that the sample addition hole moves above the next culture hole in the same vertical column for sample addition. The front and back movement of the moving plate causes the shielding cloth to move, covering the sampled culture hole. This device can remind the operator to sample the culture holes in the same vertical column through the auxiliary plate, and at the same time, the sampled culture holes in the same vertical column will be covered after sampling, avoiding the situations of missed sample addition and repeated sample addition during cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 is the isometric view of the present utility model;

[0014] Figure 2 is the right sectional view of the partial structure of the present utility model;

[0015] Figure 3 is the top view of the present utility model;

[0016] Figure 4 is the structural schematic diagram of the fixing component in Embodiment 1 of the present utility model;

[0017] Figure 5 is the partial structural schematic diagram of the present utility model;

[0018] Figure 6 is the structural schematic diagram of the fixing component in Embodiment 2 of the present utility model.

[0019] In the figure, 1 is the main body of the culture plate; 2 is the mounting rack; 3 is the identification plate; 4 is the connecting rod; 5 is the auxiliary plate; 6 is the auxiliary rack; 7 is the support shaft; 8 is the elastic member; 9 is the shielding cloth; 10 is the moving plate; 11 is the sample addition hole; 12 is the identification frame; 13 is the support frame; 14 is the threaded groove; 15 is the bolt; 16 is the knob; 17 is the first magnet; 18 is the second magnet. Detailed implementation manner

[0020] The following further elaborates on the present utility model with reference to the accompanying drawings.

[0021] This specific embodiment is merely an interpretation of the present utility model and is not a limitation thereof. After reading this specification, those skilled in the art may make modifications to this embodiment without creative contributions as needed, but as long as it is within the scope of the claims of the present utility model, it is protected by the patent law.

[0022] Embodiment 1:

[0023] Refer to Figures 1-4, A cell culture plate sample loading positioning device, including a culture plate body 1. A support frame 13 is fixedly arranged on the surface of the culture plate body 1. A fixing component is arranged inside the support frame 13. The support frame 13 is provided with a mounting frame 2 through the fixing component. A marking plate 3 is fixedly arranged inside the mounting frame 2. Two connecting rods 4 are fixedly arranged inside the mounting frame 2. The two connecting rods 4 are symmetrically distributed on the front and rear sides of the mounting frame 2. The front and rear sides of the auxiliary plate 5 are supported during movement by the two connecting rods 4, preventing the auxiliary plate 5 from tilting and deforming due to uneven force on its surface. A sliding auxiliary plate 5 is arranged on the surface of the connecting rod 4. An auxiliary frame 6 is fixedly arranged on the top of the auxiliary plate 5. A support shaft 7 is rotatably arranged inside the auxiliary frame 6. An elastic member 8 is arranged on the surface of the support shaft 7. The elastic member 8 is a coil spring. One end of the elastic member 8 is fixedly connected to the surface of the support shaft 7, and the other end of the elastic member 8 is fixedly connected to the auxiliary frame 6. The coil spring has good elasticity. Therefore, when the support shaft 7 rotates, it will drive the elastic member 8 to contract. After the surface of the elastic member 8 is not stressed, the elastic member 8 will rebound and drive the support shaft 7 to rotate, thereby winding up the unfolded shielding cloth 9. The shielding cloth 9 is wound around the surface of the support shaft 7. One end of the shielding cloth 9 is fixedly connected to the support shaft 7, and the other end of the shielding cloth 9 is fixedly provided with a moving plate 10. A sample loading hole 11 is opened on the surface of the moving plate 10. For this cell culture plate sample loading positioning device, the auxiliary plate 5 and the moving plate 10 are moved to the specified vertical column to be sampled. Then, the sample loading port of the sample loading device is placed inside the sample loading hole 11. Subsequently, the sample loading port of the sample loading device adds the reagent into the culture hole at the bottom of the sample loading hole 11. Then, the moving plate 10 is pulled to move, so that the sample loading hole 11 moves above the next culture hole in the same vertical column for sample loading. The front and back movement of the moving plate 10 causes the shielding cloth 9 to move, covering the sampled culture hole. This device can remind the operator to sample the culture holes in the same vertical column through the auxiliary plate 5. At the same time, the sampled culture holes in the same vertical column will be covered after sampling, avoiding the situations of missed sampling and repeated sampling during cell culture.

[0024] Specifically, the fixing component includes a threaded groove 14 opened on the surface of the support frame 13. A bolt 15 is threadedly arranged inside the threaded groove 14. The bolt 15 is threadedly connected to the mounting frame 2. A knob 16 is fixedly arranged on the top of the bolt 15. The knob 16 is made of rubber. Place the mounting frame 2 on the support frame 13 and rotate the knob 16, so that the bolt 15 rotates into the mounting frame 2 and the threaded groove 14 in sequence, thereby fixing the mounting frame 2 and the support frame 13, preventing the mounting frame 2 from moving on the support frame 13 during sample loading, and thus avoiding sample loading failure.

[0025] Specifically, a marking frame 12 is fixedly arranged at the top of the identification board 3. The number of the marking frames 12 is multiple. The inside of the marking frame 12 can be marked by increasing Arabic numerals from left to right in sequence, so as to remind the operator which column is being sampled now and which column has completed sampling, thereby indicating subsequent repeated sampling and missed sampling.

[0026] Working principle: Place the mounting bracket 2 on the support frame 13, rotate the knob 16, so that the bolt 15 rotates into the mounting bracket 2 and the threaded groove 14 in sequence, thereby fixing between the mounting bracket 2 and the support frame 13. Subsequently, the auxiliary plate 5 can be pulled to move on the surface of the connecting rod 4. The movement of the auxiliary plate 5 drives the moving plate 10 to move to the designated vertical column to be sampled. The position of the vertical column can be known through the marking inside the marking frame 12. Place the sampling port of the sampling device into the sampling hole 11. Subsequently, the sampling port of the sampling device adds the reagent into the culture hole of the culture plate body 1 at the bottom of the sampling hole 11. Then, pull the moving plate 10 to move, so that the sampling hole 11 moves above the next culture hole in the same vertical column, and then sample the next culture hole. At the same time, the movement of the moving plate 10 makes the shielding cloth 9 move, thereby making the support shaft 7 rotate. The rotation of the support shaft 7 compresses the elastic member 8. When the sampling of this vertical column is completed, move the moving plate 10 back to its original position. Under the elastic return action of the elastic member 8, the support shaft 7 will be driven to rotate to wind the shielding cloth 9. Subsequently, the sampling port of the sampling device leaves the moving plate 10, and the auxiliary plate 5 is pushed to move to the next vertical column for sampling.

[0027] Embodiment 2:

[0028] As Figure 5 and Figure 6 shown, a cell culture plate sampling positioning device. The difference between Embodiment 2 and Embodiment 1 lies in the different fixing components. The fixing component in Embodiment 2 is that the fixing component includes a first magnet 17 fixedly arranged inside the support frame 13, and a second magnet 18 is fixedly arranged inside the mounting bracket 2. The bottom end of the second magnet 18 is connected to the first magnet 17. The first magnet 17 and the second magnet 18 are made of the same material, both made of a weak magnetic material.

[0029] The principle of this embodiment is similar to that of Embodiment 1. The main difference is that when installing the mounting bracket 2, only need to align the second magnet 18 with the annular groove above the support frame 13, and then place the second magnet 18 into it to connect with the first magnet 17, then the support frame 13 and the mounting bracket 2 can be fixed. The support frame 13 and the mounting bracket 2 can be quickly fixed by the two magnets, thereby simplifying the process of cell culture. The principles of other parts are the same as those of Embodiment 1 and will not be elaborated here.

Claims

1. A cell culture plate sample addition positioning device, comprising a culture plate body (1), characterized in that, A support frame (13) is fixedly arranged on the surface of the culture plate body (1). A fixing component is arranged inside the support frame (13). The support frame (13) is provided with a mounting frame (2) through the fixing component. A marking plate (3) is fixedly arranged inside the mounting frame (2). A connecting rod (4) is fixedly arranged inside the mounting frame (2). An auxiliary plate (5) is slidably arranged on the surface of the connecting rod (4). An auxiliary frame (6) is fixedly arranged at the top of the auxiliary plate (5). A support shaft (7) is rotatably arranged inside the auxiliary frame (6). An elastic member (8) is arranged on the surface of the support shaft (7). A shielding cloth (9) is wound around the surface of the support shaft (7). One end of the shielding cloth (9) is fixedly connected to the support shaft (7). The other end of the shielding cloth (9) is fixedly provided with a moving plate (10). A sample adding hole (11) is formed in the surface of the moving plate (10).

2. The cell culture plate sample addition positioning device according to claim 1, characterized in that: The fixing component includes a threaded groove (14) formed on the surface of the support frame (13). A bolt (15) is threadedly arranged inside the threaded groove (14). The bolt (15) is threadedly connected to the mounting frame (2).

3. The cell culture plate sample addition positioning device according to claim 2, characterized in that: A marking frame (12) is fixedly arranged at the top of the marking plate (3). The number of the marking frames (12) is multiple.

4. The cell culture plate sample addition positioning device according to claim 1, characterized in that: The elastic member (8) is a torsion spring. One end of the elastic member (8) is fixedly connected to the surface of the support shaft (7). The other end of the elastic member (8) is fixedly connected to the auxiliary frame (6).

5. The cell culture plate sample addition positioning device according to claim 2, wherein: A knob (16) is fixedly arranged at the top of the bolt (15). The knob (16) is made of rubber.

6. The cell culture plate sample loading positioning device according to claim 1, wherein: The number of the connecting rods (4) is two, and the two connecting rods (4) are symmetrically distributed on the front and rear sides of the mounting frame (2).

7. A cell culture plate sample addition positioning device according to claim 1, characterized in that: The fixing component includes a first magnet (17) fixedly arranged inside the support frame (13). A second magnet (18) is fixedly arranged inside the mounting frame (2). The bottom end of the second magnet (18) is connected to the first magnet (17).

8. A cell culture plate sample addition positioning device according to claim 7, characterized in that: The first magnet (17) and the second magnet (18) are made of the same material, both made of a weak magnetic material.

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