Device for measuring antibacterial property of volatile substance

Abstract

The utility model relates to the technical field of biological experiment tools, in particular to a volatile substance antibacterial property measuring device which comprises a first shell, a second shell and an air supply device, the first shell is provided with a first cavity used for containing biocontrol bacteria and / or volatile medicine, and the second shell is provided with a second cavity used for containing biocontrol bacteria and / or volatile medicine. The first shell is provided with a first cavity used for containing pathogenic bacteria and / or plants carrying the pathogenic bacteria, the second shell is provided with a second cavity used for containing the pathogenic bacteria and / or plants carrying the pathogenic bacteria, the first cavity is communicated with the second cavity in a sealed mode, and the air supply device is used for generating flowing of gas flowing into the second cavity from the first cavity.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiment utensils, and particularly relates to a device for measuring the antibacterial property of volatile substances. Background Art

[0002] In the past few decades, volatile metabolomics has developed vigorously. In the early stage, people mainly explored the complex mechanisms of the interactions between plants and plants, plants and insects, and insects and insects. In recent years, since the volatile substances produced by bacteria or fungi can also produce induction effects between plants or insects, people have begun to study the inhibitory effects of the volatile metabolites of biocontrol bacteria on pathogenic bacteria or pests and the induced resistance effects of the volatile metabolites of biocontrol bacteria on plants.

[0003] At present, the antibacterial activity of microbial volatile metabolites is generally detected by using a two-compartment dish (there is a partition in the middle of the culture dish, which plays a role of physical isolation), or by buckling the bottoms of two dishes. However, this test method is inconvenient to operate and the detection results are not accurate enough. In order to improve the accuracy of the detection results, Chinese patent document CN116606721A discloses a special culture dish for detecting the antibacterial activity of volatile organic compounds, which includes a base, a lid and a heating unit. The heating unit includes a mounting seat, a rotating assembly, a rotating disk, mounting arms, a column rod, a heating plate, heating wires, a bracket and a sealing cover. After injecting volatile organic compounds into the column rod, the lid is nested above the base and fixed by the fixing bolts. Then, the rotating assembly is started to drive the rotating disk to rotate, so that the mounting arms on the rotating disk rotate, and the column rod makes a circular motion around the center of the rotating disk. After rotating to between the sealing cover and the heating plate, the heating wires are started, and the heating plate is used to heat the volatile organic compounds in the column rod. When the volatile organic compounds are heated to an appropriate temperature detected by the temperature sensor, the rotating assembly is started, and through the mounting arms, the column rod is driven to move out from between the sealing cover and the heating plate. Then, the heated volatile organic compounds in the column rod quickly volatilize and fill the culture dish to inhibit pathogenic bacteria. With the above structure, different volatile organic compounds can be heated to an appropriate temperature, and the adaptability is stronger. However, the special culture dish disclosed in this patent document is not suitable for measuring the antibacterial properties of temperature-sensitive volatile substances or biocontrol bacteria. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a device for measuring the antibacterial property of volatile substances, which can solve the problem that the current device for measuring the antibacterial property of volatile substances is not suitable for temperature-sensitive volatile substances or biocontrol bacteria.

[0005] In order to achieve the above purpose, the technical solution adopted by the device for measuring the antibacterial property of volatile substances of the utility model is as follows:

[0006] A device for measuring the antibacterial property of a volatile substance, comprising a first housing, a second housing and a blowing device. The first housing has a first cavity for placing biocontrol bacteria and / or volatile drugs. The second housing has a second cavity for placing pathogenic bacteria and / or plants carrying pathogenic bacteria. The first cavity and the second cavity are sealed and communicated with each other. The blowing device is used to generate the flow of gas flowing from the first cavity into the second cavity.

[0007] The device for measuring the antibacterial property of the volatile substance of the present utility model has a simple structure, can be mass-produced, and does not need to heat the biocontrol bacteria and / or volatile drugs during use. It is suitable for measuring the antibacterial property of volatile substances or biocontrol bacteria sensitive to temperature. The device for measuring the antibacterial property of the volatile substance of the present utility model is in a sealed state during operation, can quantitatively reference the number of biocontrol bacteria and the concentration of volatile substances, can ensure the quality of volatile substances and the accuracy of measurement results, and provides better services for the research on the antibacterial property of volatile substances.

[0008] Preferably, the first housing includes a first cylindrical shell, a first end cover and a second end cover. The first end cover is hermetically connected to the first cylindrical shell, and the second end cover is detachably hermetically connected to the first cylindrical shell. The space surrounded by the first cylindrical shell, the first end cover and the second end cover forms the first cavity.

[0009] Preferably, the second end cover is threadedly connected to the first cylindrical shell.

[0010] Preferably, the second housing includes a second cylindrical shell and a third end cover. The third end cover is detachably hermetically connected to the second cylindrical shell, and the second cylindrical shell is hermetically connected to the second end cover. The space surrounded by the second cylindrical shell, the second end cover and the third end cover forms the second cavity.

[0011] Preferably, the third end cover is threadedly connected to the second cylindrical shell.

[0012] Preferably, a through hole is formed in the second end cover, and the first cavity and the second cavity are communicated through the through hole.

[0013] Preferably, the blowing device includes an impeller and a motor. The motor has a rotating shaft, and the impeller is connected to the rotating shaft. The impeller can rotate around the rotating shaft under the drive of the motor, and the impeller is located in the first cavity.

[0014] Preferably, the rotation direction of the impeller intersects with the center line of the first housing; the diameter of the impeller is 1 / 4 to 1 / 2 of the diameter of the first cavity.

[0015] Preferably, the materials of the first housing and the second housing are both transparent materials.

[0016] Preferably, the measuring device for the antibacterial property of the volatile substance further includes a first petri dish and a second petri dish. The first petri dish is placed in the first cavity and is used for placing biocontrol bacteria and / or volatile drugs; the second petri dish is placed in the second cavity and is used for placing pathogenic bacteria and / or plants carrying pathogenic bacteria. Description of the Drawings

[0017] Figure 1 It is a schematic diagram of the state of the measuring device for the antibacterial property of the volatile substance in the embodiment of the present utility model during operation;

[0018] Figure 2 It is a schematic structural diagram of the measuring device for the antibacterial property of the volatile substance in the embodiment of the present utility model;

[0019] Figure 3 It is a schematic structural diagram of the second end cover with a through hole in the measuring device for the antibacterial property of the volatile substance in the embodiment of the present utility model;

[0020] The reference numerals are as follows: 1 - first end cover; 2 - second petri dish; 3 - second cylindrical shell; 4 - second end cover; 5 - impeller; 6 - first cylindrical shell; 7 - first petri dish; 8 - wire; 9 - external power supply; 10 - through hole; 11 - third end cover. Detailed Embodiments

[0021] The measuring device for the antibacterial property of the volatile substance of the present utility model is a pioneering invention. The measuring device for the antibacterial property of the volatile substance of the present utility model includes a first housing, a second housing, and a air supply device. The first housing has a first cavity for placing biocontrol bacteria and / or volatile drugs, the second housing has a second cavity for placing pathogenic bacteria and / or plants carrying pathogenic bacteria, the first cavity and the second cavity are hermetically communicated, and the air supply device is used to generate a flow of gas flowing from the first cavity into the second cavity. The first housing is used to place biocontrol bacteria and / or volatile drugs, the second housing is used to place pathogenic bacteria and / or plants carrying pathogenic bacteria, and the air supply device is used to generate an air flow to accelerate the volatile substance generated by the biocontrol bacteria and / or volatile drugs to enter the second cavity from the first cavity. The measuring device for the antibacterial property of the volatile substance of the present utility model has a simple structure and can be mass-produced. Specifically, during implementation, a device with a corresponding size can be selected according to the size of the object to be measured. In addition, the measuring device for the antibacterial property of the volatile substance of the present utility model is in a sealed state during operation, can provide a quantitative reference for the number of biocontrol bacteria and the concentration of the volatile substance, can ensure the quality of the volatile substance and the accuracy of the measurement result, and provides better services for the research on the antibacterial property of the volatile substance.

[0022] In some preferred embodiments, the volume of the first cavity is 5 to 10 times the volume of the biocontrol bacteria and / or volatile drugs.

[0023] In some preferred embodiments, the volume of the second cavity is 2 to 7 times the volume of the pathogenic bacteria and / or the plants carrying the pathogenic bacteria.

[0024] In some preferred embodiments, the first housing includes a first cylindrical shell, a first end cap, and a second end cap. The first end cap is hermetically connected to the first cylindrical shell, the second end cap is removably and hermetically connected to the first cylindrical shell, and the space enclosed by the first cylindrical shell, the first end cap, and the second end cap forms the first cavity.

[0025] In some preferred embodiments, the first end cap and the first cylindrical shell are integrally formed.

[0026] In some preferred embodiments, the second end cap and the first cylindrical shell are threadedly connected.

[0027] In some preferred embodiments, the second housing includes a second cylindrical shell and a third end cap. The third end cap is removably and hermetically connected to the second cylindrical shell, the second cylindrical shell is hermetically connected to the second end cap, and the space enclosed by the second cylindrical shell, the second end cap, and the third end cap forms the second cavity.

[0028] In some preferred embodiments, the third end cap and the second cylindrical shell are threadedly connected.

[0029] In some preferred embodiments, the second cylindrical shell and the second end cap are adhesively bonded with hot melt adhesive.

[0030] In some preferred embodiments, a through hole is formed in the second end cap, and the first cavity and the second cavity are communicated through the through hole.

[0031] In some preferred embodiments, the aperture of the through hole is 4 to 6 mm, and the number of the through holes is 40 to 60.

[0032] In some preferred embodiments, the air supply device includes an impeller and a motor. The motor has a rotating shaft, the impeller is connected to the rotating shaft, the impeller can rotate around the rotating shaft under the drive of the motor, and the impeller is located in the first cavity.

[0033] In some preferred embodiments, the rotation direction of the impeller intersects with the center line of the first housing.

[0034] In some preferred embodiments, the diameter of the impeller is 1 / 4 to 1 / 2 of the diameter of the first cavity.

[0035] In some preferred embodiments, the materials of the first housing and the second housing are both transparent materials. For example, the materials of the first housing and the second housing are plexiglass.

[0036] In some preferred embodiments, the apparatus for measuring the antibacterial property of volatile substances further includes a first culture dish and a second culture dish. The first culture dish is placed in the first cavity and is used for placing biocontrol bacteria and / or volatile drugs. The second culture dish is placed in the second cavity and is used for placing pathogenic bacteria and / or plants carrying pathogenic bacteria.

[0037] The technical solution of the present utility model will be further described below in conjunction with specific embodiments.

[0038] Embodiment

[0039] The apparatus for measuring the antibacterial property of volatile substances in this embodiment includes a first housing, a second housing, and a ventilation device. The first housing has a first cavity for placing biocontrol bacteria and / or volatile drugs. The second housing has a second cavity for placing pathogenic bacteria and / or plants carrying pathogenic bacteria. The first cavity and the second cavity are hermetically connected. The ventilation device is used to generate a flow of gas flowing from the first cavity into the second cavity. The first housing is used to place biocontrol bacteria and / or volatile drugs, the second housing is used to place pathogenic bacteria and / or plants carrying pathogenic bacteria, and the ventilation device is used to generate an air flow to accelerate the volatile substances produced by the biocontrol bacteria and / or volatile drugs to enter the second cavity. The schematic diagram of the state of the apparatus for measuring the antibacterial property of volatile substances in this embodiment during operation is as shown in Figure 1 shown. The schematic structural diagram of the apparatus for measuring the antibacterial property of volatile substances in this embodiment is as shown in Figure 2 shown.

[0040] The first housing includes a first cylindrical shell 6, a first end cover 11, and a second end cover 4. The first end cover 11 and the first cylindrical shell 6 are hermetically connected. The second end cover 4 and the first cylindrical shell 6 are detachably hermetically connected. The space surrounded by the first cylindrical shell 6, the first end cover 11, and the second end cover 4 forms the first cavity. Both the first end cover 11 and the second end cover 4 are circular. The first end cover 11 and the first cylindrical shell 6 can be connected by threads or integrally formed. In this embodiment, the first end cover 11 and the first cylindrical shell 6 are integrally formed. The second end cover 4 and the first cylindrical shell 6 can be connected by bolts or threads. In this embodiment, the second end cover 4 and the first cylindrical shell 6 are connected by threads. The first cylindrical shell 6, the first end cover 11, and the second end cover 4 are all made of transparent organic glass material.

[0041] The second housing includes a second cylindrical shell 3 and a third end cap 1. The third end cap 1 and the second cylindrical shell 3 are detachably and sealingly connected. The second cylindrical shell 3 and the second end cap 4 are sealingly connected. The space formed by the second cylindrical shell 3, the second end cap 4 and the third end cap 1 constitutes a second cavity. The third end cap 1 is circular. The third end cap 1 and the second cylindrical shell 3 can be connected by bolts or by screw threads. In this embodiment, the third end cap 1 and the second cylindrical shell 3 are connected by screw threads. The second cylindrical shell 3 and the second end cap 4 can be connected by bolts, by screw threads or by an adhesive. In this embodiment, the second cylindrical shell 3 and the second end cap 4 are bonded by hot melt adhesive. Both the second cylindrical shell 3 and the third end cap 1 are made of transparent organic glass material.

[0042] The air supply device includes an impeller 5 and a motor. The motor has a rotating shaft. The impeller 5 is connected to the rotating shaft. The impeller 5 can rotate around the rotating shaft under the drive of the motor. The impeller 5 is located in the first cavity. The rotation direction of the impeller 5 is perpendicular to the central axis of the first housing. The diameter of the impeller 5 can be 1 / 4 to 1 / 2 of the diameter of the first cavity. In this embodiment, the diameter of the impeller 5 is 1 / 4 of the diameter of the first cavity. The air supply device can be self - contained with a battery or can be connected to an external power source through a wire. The external power source can be a DC power source or an AC power source. In this embodiment, the air supply device further includes a wire 8. One end of the wire 8 is connected to the motor and the other end is connected to an external power source 9.

[0043] In the volatile substance antibacterial property measuring device of this embodiment, the structural schematic diagram of the second end cap 4 with a through - hole is as Figure 3 shown. A through - hole 10 is provided on the second end cap 4 (the diameter of the through - hole 10 can be increased, but it is necessary to ensure that the test materials in the first cavity will not fall off and the fan can be normally installed. The through - holes 10 are arranged densely to ensure good gas flow between the cavities). The first cavity and the second cavity are connected through the through - hole 10. The aperture of the through - hole 10 can be 4 - 6 mm, and the number of the through - holes 10 can be 40 - 60. In this embodiment, the aperture of the through - hole 10 is 6 mm and the number of the through - holes 10 is 49.

[0044] The volatile substance antibacterial property measuring device of this embodiment further includes a first culture dish 7 and a second culture dish 2. The first culture dish 7 is placed in the first cavity (specifically placed on the first end cap 11) for placing biocontrol bacteria and / or volatile drugs; the second culture dish 2 is placed in the second cavity (specifically placed on the second end cap 4) for placing pathogenic bacteria and / or plants carrying pathogenic bacteria. The volume of the first cavity is 5 - 10 times the volume of the first culture dish 7. In this embodiment, the volume of the first cavity is 6 times the volume of the first culture dish 7. The volume of the second cavity is 5 - 10 times the volume of the second culture dish 2. In this embodiment, the volume of the second cavity is 7 times the volume of the second culture dish 2.

[0045] The working principle of the device for measuring the antibacterial property of volatile substances in this embodiment is as follows: First, disinfect and sterilize the first housing, the second housing, the air supply device in the first housing, the first petri dish 7 and the second petri dish 2. Then, inoculate the pathogenic bacteria into the second petri dish 2 and inoculate the biocontrol bacteria into the first petri dish 7. Next, place the second petri dish 2 on the second end cap 4 and place the first petri dish 7 on the first end cap 11. Then, thread the third end cap 1 and the second cylindrical shell 3 together, and thread the second end cap 4 and the first cylindrical shell 6 together to complete the assembly of the device. Then, connect the wire 8 to an external power supply 9, turn on the motor, and make the impeller 5 rotate around the rotating shaft under the drive of the motor to generate an air flow to accelerate the volatile substances produced by the biocontrol bacteria and / or volatile drugs to enter the second cavity from the first cavity through the through hole 10. After culturing for a certain period of time, observe the change in the colony diameter of the pathogenic bacteria in the second petri dish 2, and the antibacterial property of the volatile metabolites of the biocontrol bacteria can be qualitatively or quantitatively determined.

Claims

1. A device for measuring the antibacterial property of a volatile substance, characterized in that, It includes a first housing, a second housing and a ventilation device. The first housing has a first cavity for placing biocontrol bacteria and / or volatile drugs. The second housing has a second cavity for placing pathogenic bacteria and / or plants carrying pathogenic bacteria. The first cavity and the second cavity are sealed and communicated with each other. The ventilation device is used to generate the flow of gas flowing from the first cavity into the second cavity.

2. The measuring device for the antibacterial property of volatile substances according to claim 1, characterized in that, The first housing includes a first cylindrical shell, a first end cover and a second end cover. The first end cover is hermetically connected to the first cylindrical shell. The second end cover is detachably and hermetically connected to the first cylindrical shell. The space surrounded by the first cylindrical shell, the first end cover and the second end cover forms the first cavity.

3. The apparatus for measuring the antibacterial property of a volatile substance according to claim 2, characterized in that, The second end cover is threadedly connected to the first cylindrical shell.

4. The measuring device for the antibacterial property of volatile substances according to claim 2, characterized in that, The second housing includes a second cylindrical shell and a third end cover. The third end cover is detachably and hermetically connected to the second cylindrical shell. The second cylindrical shell is hermetically connected to the second end cover. The space surrounded by the second cylindrical shell, the second end cover and the third end cover forms the second cavity.

5. The apparatus for measuring the antibacterial property of volatile substances according to claim 4, characterized in that, The third end cover is threadedly connected to the second cylindrical shell.

6. The measuring device for the antibacterial property of volatile substances according to any one of claims 2-5, characterized in that A through hole is provided on the second end cover. The first cavity and the second cavity are communicated through the through hole.

7. The measuring device for the antibacterial property of volatile substances according to claim 1, characterized in that, The ventilation device includes an impeller and a motor. The motor has a rotating shaft. The impeller is connected to the rotating shaft. The impeller can rotate around the rotating shaft under the drive of the motor. The impeller is located in the first cavity.

8. The measuring device for the antibacterial property of volatile substances according to claim 7, characterized in that, The rotation direction of the impeller intersects with the center line of the first housing. The diameter of the impeller is 1 / 4 to 1 / 2 of the diameter of the first cavity.

9. The measuring device for the antibacterial property of volatile substances according to claim 1, characterized in that, The materials of the first housing and the second housing are both transparent materials.

10. The apparatus for measuring the antibacterial property of volatile substances according to claim 1, characterized in that, The device for measuring the antibacterial property of the volatile substance further includes a first culture dish and a second culture dish. The first culture dish is placed in the first cavity and is used for placing biocontrol bacteria and / or volatile drugs. The second culture dish is placed in the second cavity and is used for placing pathogenic bacteria and / or plants carrying pathogenic bacteria.

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