Exosome sample enrichment device
By designing a clamped rotary base and an exosome sample enrichment device for the introduction assembly, the problem of uneven contact between the precipitant and the exosome solution is solved, and a more efficient enrichment effect is achieved.
Patent Information
- Application Number
- CN202422245344.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-13
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2034-09-13
AI Technical Summary
When the existing enrichment device adds the precipitant, the precipitant and the exosome solution are unevenly in contact, resulting in poor enrichment effect.
An exosome sample enrichment device including a placement rack, a clamped rotary base, an enrichment container and an introduction assembly is designed. The clamped rotary base drives the enrichment container to rotate simultaneously, and the introduction assembly is used to achieve uniform introduction and rotation mixing of the precipitant to ensure that the solution and the precipitant are in full contact.
The contact sufficiency between the exosome sample solution and the precipitant is improved, and the enrichment effect and efficiency are enhanced.
Smart Images
Figure CN223134442U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field, and specifically relates to an exosome sample enrichment device. Background Technique
[0002] Exosomes refer to small membrane vesicles (30 - 150 nm) containing complex RNA and proteins. Currently, they specifically refer to discoidal vesicles with a diameter of 40 - 100 nm. Exosomes play an important role in physiological and pathological processes such as antigen presentation in immunity, tumor growth and migration, and tissue damage repair. At the same time, exosomes secreted by different cells have different compositions and functions, and can be used as biomarkers for disease diagnosis. All cultured cell types can secrete exosomes, and exosomes naturally exist in body fluids, including blood, saliva, urine, cerebrospinal fluid, and milk. The function of exosomes depends on the cell type from which they originate, and they can participate in various aspects such as the body's immune response, antigen presentation, cell migration, cell differentiation, and tumor invasion. The isolation and purification of exosomes have always been a concern for scientific researchers. Obtaining highly pure exosomes is crucial for subsequent research. When conducting experiments on exosome samples, it is often necessary to enrich the exosome samples. Enrichment refers to the operation step of collecting trace elements to be measured from a large amount of parent material into a smaller volume, so as to increase their content above the lower limit of determination. The coprecipitation enrichment method is to add a precipitant to the test solution. When a precipitate is formed, the trace elements are coprecipitated and precipitated out. The precipitate is filtered out and dissolved in a small volume of solvent to enrich the trace substances.
[0003] However, the existing enrichment devices have the following problems in the process of enriching exosomes: When adding the precipitant, the existing enrichment devices generally directly introduce the precipitant into the exosome solution. Such an introduction method is likely to cause the solution and the precipitant not to be fully and evenly contacted, resulting in insufficient enrichment inside the test solution and affecting the enrichment effect. Therefore, corresponding technical solutions need to be designed to solve the existing technical problems. Content of the Utility Model
[0004] The purpose of the utility model is to provide an exosome sample enrichment device, which solves the technical problem that when adding the precipitant, the existing enrichment devices generally directly introduce the precipitant into the exosome solution. Such an introduction method is likely to cause the solution and the precipitant not to be fully and evenly contacted, resulting in insufficient enrichment inside the test solution and affecting the enrichment effect.
[0005] To achieve the above object, the present utility model provides the following technical solutions: An exosome sample enrichment device, comprising a placement rack, a clamping rotary base, an enrichment container, and an introduction component. The placement rack includes two sets of discs symmetrically arranged up and down and two arc-shaped vertical plates fixed between the two sets of discs. An installation opening is formed in the middle of the lower disc. The clamping rotary base is installed in the installation opening. The clamping rotary base includes a placement disc and a motor located below the placement disc. The motor is fixed on the lower disc and the power output end is connected to the placement disc. Four sets of clamping pieces are distributed on the edge of the placement disc and a placement groove is formed inside. The enrichment container is placed in the placement groove and fixed by the four sets of clamping pieces. A through hole is provided on the upper disc. Two sets of grooves are formed by machining on both sides of the through hole. The introduction component passes through the through hole and the lower end is located inside the enrichment container. The introduction component includes an introduction pipe, a limit plate, an inner cylinder, and a rotary outer cylinder. A cover plate is provided on one side of the introduction pipe and both sides are respectively connected to the two sets of limit plates. The inner cylinder is fixed below the introduction pipe. The rotary outer cylinder is rotatably sleeved outside the inner cylinder.
[0006] As a preferred embodiment of the present utility model, the placement groove is in a bowl-shaped structure and the height in the middle of the inner placement groove is lower than the peripheral height.
[0007] As a preferred embodiment of the present utility model, the clamping piece is made of elastic metal material and is in an arc-shaped structure. The upper end of the clamping piece is in contact with the enrichment container and the lower end is in a stacked structure.
[0008] As a preferred embodiment of the present utility model, the enrichment container includes a test tube container and a cover installed on the top of the test tube container. A lead-in port is formed in the middle of the cover. The lower end of the introduction component passes through the lead-in port.
[0009] As a preferred embodiment of the present utility model, the inner cylinder is in a cylindrical structure and two sets of embedded openings are formed on the surface. A discharge net is placed in the embedded opening. The surface of the discharge net is in a porous structure.
[0010] As a preferred embodiment of the present utility model, the rotary outer cylinder includes a top ring and two sets of arc-shaped side plates symmetrically fixed below the top ring. Two openings are formed between the two sets of arc-shaped side plates. The width of the arc-shaped side plate is greater than the width of the discharge net.
[0011] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0012] 1. The enrichment device designed in this solution can achieve efficient and uniform introduction of the precipitant, improve the sufficiency of contact between the exosome sample solution and the precipitant, and cooperate with the clamping rotary base at the bottom to drive the enrichment container to rotate synchronously while introducing the precipitant, so that the precipitant and the exosome sample solution are better fused, ensuring the enrichment effect of the device.
[0013] 2. The enrichment device designed in this solution can achieve the uniform introduction and rotational mixing of the precipitant, improving the enrichment efficiency. Brief Description of the Drawings
[0014] Figure 1 It is a structural diagram of the present utility model;
[0015] Figure 2 It is a structural diagram of the separation state of the inner cylinder and the rotating outer cylinder of the present utility model;
[0016] Figure 3 It is a sectional view of the distribution of the inner cylinder and the rotating outer cylinder of the present utility model;
[0017] Figure 4 It is a structural diagram of the clamping piece of the present utility model.
[0018] In the figure: 1, disc body; 2, arc-shaped vertical plate; 3, installation opening; 4, placement tray; 5, motor; 6, clamping piece; 7, placement groove; 8, through opening; 9, groove; 10, introduction pipe; 11, limit plate; 12, inner cylinder; 13, rotating outer cylinder; 14, cover plate; 15, test tube container; 16, sealing cover; 17, introduction port; 18, embedded opening; 19, discharge mesh; 20, top ring; 21, arc-shaped side plate; 22, opening. Detailed Embodiment
[0019] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.
[0020] Please refer to Figures 1-4, the present utility model provides a technical solution: an exosome sample enrichment device, which includes a placement rack, a clamping rotary base, an enrichment container, and an introduction component. The placement rack includes two sets of disks 1 symmetrically arranged up and down and two arc-shaped vertical plates 2 fixed between the two sets of disks 1. An installation opening 3 is formed in the middle of the disk 1 located below, and the clamping rotary base is installed in the installation opening 3. The clamping rotary base includes a placement disk 4 and a motor 5 located below the placement disk 4. The motor 5 is fixed on the lower disk 1 and the power output end is connected to the placement disk 4. Four groups of clamping pieces 6 are distributed on the edge of the placement disk 4 and a placement groove 7 is formed inside. The enrichment container is placed inside the placement groove 7 and fixed by the four groups of clamping pieces 6. A through opening 8 is provided on the disk 1 located above. Two groups of grooves 9 are formed by processing on both sides of the through opening 8. The introduction component passes through the through opening 8 and the lower end is located inside the enrichment container. The introduction component includes an introduction tube 10, a limiting plate 11, an inner cylinder 12, and a rotating outer cylinder 13. A cover plate 14 is provided on one side of the introduction tube 10 and both sides are respectively connected to the two groups of limiting plates 11. The inner cylinder 12 is fixed below the introduction tube 10, and the rotating outer cylinder 13 is rotatably sleeved outside the inner cylinder 12.
[0021] Further improved, as Figure 1 shown, the placement groove 7 is in a bowl-shaped structure and the height in the middle of the placement groove 7 inside is lower than the peripheral height, which is convenient for placing the bottom of the enrichment container in the placement groove 7.
[0022] Further improved, as Figure 4 shown, the clamping piece 6 is made of elastic metal material and is in an arc-shaped structure. The upper end of the clamping piece 6 is in contact with the enrichment container and the lower end is in a stacked structure. The enrichment container can be fixed by clamping through the four groups of clamping pieces 6.
[0023] Further improved, as Figure 1 shown, the enrichment container includes a test tube container 15 and a cover 16 installed on the top of the test tube container 15. A guide inlet 17 is provided in the middle of the cover 16, and the lower end of the introduction component passes through the guide inlet 17. In this way, the closed introduction method reduces the influence of the external environment.
[0024] Further improved, as Figure 2 and 3 shown, the inner cylinder 12 is in a cylindrical structure and two sets of embedded openings 18 are provided on the surface. A discharge mesh 19 is installed in the embedded openings 18. The surface of the discharge mesh 19 is in a porous structure, which is convenient for externally guiding the precipitant powder and introducing and fusing the solution, and improves the uniformity of the introduction of the precipitant powder.
[0025] Specifically, the rotating outer cylinder 13 includes a top ring 20 and two groups of arc-shaped side plates 21 symmetrically fixed below the top ring 20, two groups of openings 22 are formed between the two groups of arc-shaped side plates 21, and the width of the arc-shaped side plates 21 is greater than the width of the discharge net 19. Through the overlapping separation of the arc-shaped side plates 21 and the discharge net 19, the precipitant powder can be sealed and externally conducted.
[0026] During use: When it is necessary to enrich the exosome sample, the staff can place the exosome sample solution in the enrichment container, close the cover 16, and then place the enrichment container in the placement groove 7 to fix the enrichment container through four sets of clamping pieces 6. Then the staff places the precipitant powder in the introduction component, inserts the introduction component from the through opening 8 and introduces it into the enrichment container from the introduction port 17. At this time, the motor 5 is turned on, and the motor 5 drives the placement plate 4 and the enrichment container to rotate, and synchronously twists the rotating outer cylinder 13, so that the arc-shaped side plate 21 is staggered with the discharge net 19. At this time, the solution and the precipitant powder are evenly dissolved, and the purpose of evenly fusion of the solution and the precipitant powder is achieved through the rotation of the enrichment container. After the fusion is completed, the motor 5 is turned off, and the exosome sample solution is naturally precipitated and enriched, which greatly improves the efficiency of enriching the exosome sample solution.
[0027] In the description of the present invention, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "the other end", "upper", "one side", "top", "inside", "front", "center", "both ends" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0028] In addition, the terms "first", "second", "third" and "fourth" are used for descriptive purposes only and are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first", "second", "third" and "fourth" may explicitly or implicitly include at least one of such features.
[0029] In the present utility model, unless otherwise clearly stipulated and limited, the terms such as "installation", "setting", "connection", "fixation" and "screw-on" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. Unless otherwise clearly defined, ordinary technicians in this field can understand the specific meanings of the above terms in the utility model according to the specific circumstances.
[0030] Finally, it should be noted that the above are only the preferred embodiments of the present utility model and are not used to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.
Claims
1. An exosome sample enrichment device, characterized in that: It includes a placement rack, a clamping rotary base, an enrichment container and an introduction component. The placement rack includes two groups of discs (1) symmetrically arranged up and down and two arc-shaped vertical plates (2) fixed between the two groups of discs (1). An installation opening (3) is formed in the middle of the disc (1) located below. The clamping rotary base is installed in the installation opening (3). The clamping rotary base includes a placement disc (4) and a motor (5) located below the placement disc (4). The motor (5) is fixed on the lower disc (1) and the power output end is connected to the placement disc (4). Four groups of clamping pieces (6) are distributed on the edge of the placement disc (4) and a placement groove (7) is formed inside. The enrichment container is placed inside the placement groove (7) and fixed by the four groups of clamping pieces (6). A through opening (8) is provided on the disc (1) located above. Two groups of grooves (9) are formed by processing on both sides of the through opening (8). The introduction component passes through the through opening (8) and the lower end is located inside the enrichment container. The introduction component includes an introduction pipe (10), a limit plate (11), an inner cylinder (12) and a rotary outer cylinder (13). A cover plate (14) is provided on one side of the introduction pipe (10) and both sides are respectively connected to the two limit plates (11). The inner cylinder (12) is fixed below the introduction pipe (10). The rotary outer cylinder (13) is rotatably sleeved outside the inner cylinder (12).
2. The exosome sample enrichment device according to claim 1, wherein: The placement groove (7) has a bowl-shaped structure and the height in the middle of the placement groove (7) inside is lower than the height of the periphery.
3. The exosome sample enrichment device according to claim 1, characterized in that: The clamping piece (6) is made of elastic metal material and has an arc-shaped structure. The upper end of the clamping piece (6) is in contact with the enrichment container and the lower end has a laminated structure.
4. The exosome sample enrichment device according to claim 3, wherein: The enrichment container includes a test tube container (15) and a cover (16) installed on the top of the test tube container (15). A guide inlet (17) is formed in the middle of the cover (16). The lower end of the introduction component passes through the guide inlet (17).
5. The exosome sample enrichment device according to claim 1, characterized in that: The inner cylinder (12) has a cylindrical structure and two embedded openings (18) are formed on the surface. A discharge net (19) is placed inside the embedded opening (18). The surface of the discharge net (19) has a porous structure.
6. The exosome sample enrichment device according to claim 5, wherein: The rotary outer cylinder (13) includes a top ring (20) and two arc-shaped side plates (21) symmetrically fixed below the top ring (20). Two openings (22) are formed between the two arc-shaped side plates (21). The width of the arc-shaped side plate (21) is greater than the width of the discharge net (19).