Strain culture system with automatic cleaning and steam disinfection functions
By designing a strain culture system for automatic cleaning and steam-elimination, the problems of unclean cleaning and insufficient disinfection in traditional strain culture are solved, and the automation and efficiency of strain culture is achieved, which is suitable for large-scale production.
Patent Information
- Application Number
- CN202422201340.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-09
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2034-09-09
AI Technical Summary
Traditional bacterial culture methods have irregular culture cycles, unclear pipeline cleaning, and insufficient disinfection of bacterial culture pipelines, resulting in low efficiency and long cycles of bacterial culture, which are not suitable for large-scale cultivation.
A strain culture system for automatic cleaning and steam purification is designed, including strain tanks, cleaning systems and steam purification systems. The cleaning of the inlet pipe and the cleaning circuit pipe is cleaned, and the steam pipe circuit and compressed air pipe are sterilized at high temperature. Combined with the hot water cleaning system and the temperature control and oxygenation function, the cleanliness and consistency of the culture system is ensured.
Automatic cleaning and efficient steam disinfection of strain culture are realized, the cleanliness and consistency of strain culture is ensured, the cultivation efficiency is improved, the cultivation cycle is shortened, and it is suitable for large-scale production.
Smart Images
Figure CN223134461U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of strain culture, in particular to a strain culture system with automatic cleaning and steam empty sterilization. Background Technique
[0002] Strain culture is widely used in the industrial production field. Traditional culture methods have certain limitations. For example, the culture process cycle is not fixed, the pipeline cleaning is not clean, and the disinfection of the strain culture pipeline is not sufficient, resulting in the multiplication factor of strain culture not reaching the expected requirements, low strain cultivation efficiency, long cycle, and not suitable for large-scale cultivation technical indicators. Content of the Utility Model
[0003] Aiming at the deficiencies of the prior art, the utility model provides a strain culture system with automatic cleaning and steam empty sterilization, which solves the problems in the above background.
[0004] To achieve the above purposes, the utility model is realized through the following technical solutions: A strain culture system with automatic cleaning and steam empty sterilization includes a strain tank, a cleaning system, a steam empty sterilization system, and a strain culture system; a tank top power assembly, a strain addition port, and a culture medium addition port are arranged at the upper end of the tank body of the strain tank; a hot water / ice water jacket is arranged on the side of the tank body, a steam / condensate jacket is arranged on the side of the conical bottom, and an inlet / outlet is arranged at the bottom; a tank body sampling port is also arranged on the side of the tank body;
[0005] The cleaning system includes a tank cleaning CIP station, a cleaning inlet pipe, and a cleaning return pipe; the cleaning inlet pipe is connected to the tank top power assembly, and the cleaning return pipe is connected to the inlet / outlet; the tank cleaning CIP station drives the cleaning liquid to flow in the pipelines of the cleaning inlet pipe and the cleaning return pipe;
[0006] The steam empty sterilization system includes a steam pipe circuit, a compressed air pipe, and a condensate output pipe; the steam pipe circuit is connected to the tank body and the steam / condensate jacket for steam sterilization; the compressed air pipe is connected to the tank body, and the condensate generated by cooling is discharged through the condensate output pipe connected to the steam / condensate jacket;
[0007] The inlet / outlet is respectively connected to a pure water pipe or a brewing water pipe and a strain output pipe.
[0008] Furthermore, the cleaning system also includes a hot water cleaning system, and the hot water cleaning system includes a hot water inlet pipe and a hot water return pipe; the hot water inlet pipe and the hot water return pipe are respectively connected to the hot water / ice water jacket to form a hot water cleaning circuit.
[0009] Furthermore, the top power assembly of the tank includes a driving motor, an oxygen filling valve pipe, a cleaning valve, a stirring shaft, and a cleaning ball; the driving motor is fixed on the tank body; the stirring shaft is arranged in the tank body and connected to the driving motor; the oxygen filling valve pipe is fixed on the tank body; the cleaning ball is arranged in the tank body and connected to the cleaning valve arranged on the tank body.
[0010] Furthermore, the upper end of the hot water / ice water jacket is communicated with a refrigerated ice water inlet pipe and a hot water inlet pipe, and the lower end of the hot water / ice water jacket is communicated with a refrigerated ice water return pipe and a hot water return pipe; pipeline switch valves are respectively arranged on the pipelines of the refrigerated ice water inlet pipe, the refrigerated ice water return pipe, the hot water inlet pipe, and the hot water return pipe to respectively form a refrigerated ice water circuit and a hot water cleaning circuit.
[0011] Furthermore, the steam pipe circuit includes a steam pipe connected to the tank body and a steam pipe connected to the steam / condensate jacket; pipeline switch valves are respectively arranged on the steam pipe connected to the tank body and the steam pipe connected to the steam / condensate jacket; the steam pipe forms a connected pipeline with the tank body and the steam / condensate jacket; the steam / condensate jacket is communicated with a condensate output pipe; an automatic air release valve, a check valve, an overflow valve, and a reflux valve are successively arranged on the condensate output pipe along the discharge direction.
[0012] Furthermore, a thermometer and a liquid level gauge are also arranged in the tank body, and a sampling valve is installed in the tank body sampling port; pipeline switch valves are respectively arranged on the pure water pipe or the brewing water pipe to control the opening and closing of the pure water pipe or the brewing water pipe, and are connected to the cross-connecting valve arranged on the first cross-connecting pipe plate, and a conical bottom switch valve and a branch switch valve are arranged on the pipeline of the inlet / outlet; a cross-connecting valve is also arranged on the first cross-connecting pipe plate, and a control valve is arranged on the strain output pipe; a flowmeter is also arranged on the strain output pipe.
[0013] Furthermore, the cleaning inlet pipe is connected to the cleaning valve in the top power assembly of the tank through a pipeline installed on the second cross-connecting pipe plate; the cross-connecting valve arranged on the second cross-connecting pipe plate is cross-connected and turned on, and the cross-connecting valve arranged on the first cross-connecting pipe plate is cross-connected and turned on; a reflux pump is arranged on the cleaning circuit pipe.
[0014] Furthermore, the compressed air pipe is connected to a pipeline installed on the first cross-connecting pipe plate accessing the top power assembly of the tank; a pneumatic valve and a flowmeter are arranged on the compressed air pipe.
[0015] Further, an expansion tank is also provided beside the strain tank; the structure of the expansion tank is the same as that of the strain tank, and the connection methods of the expansion tank and the strain tank to the steam pipe circuit, the refrigerated ice water return pipe, the hot water return pipe, the refrigerated ice water inlet pipe, the hot water inlet pipe, the condensate output pipe, the compressed air pipe, the strain output pipe, the cleaning circuit pipe, the pure water pipe, and the brewing water pipe are the same; cross-connection valve plates are respectively provided on the second cross-connection valve plates of the expansion tank and the strain tank (A100); the cross-connection valve plates on the expansion tank and the strain tank connect the strain output pipes of the expansion tank and the strain tank, the cross-connection valve plates on the first cross-connection valve plates of the expansion tank are cross-connected and turned on, and the cross-connection valve plates on the first cross-connection valve plates of the strain tank are cross-connected and turned on, so that the tank bodies of the strain tank and the expansion tank are connected; a cross-connection valve plate is also provided on the second cross-connection valve plate, and the cross-connection valve plate is cross-connected and turned on; the capacity of the expansion tank is larger than that of the strain tank.
[0016] Further, multiple strain tanks and expansion tanks are provided, and the strain tanks and the expansion tanks are connected to the steam pipe circuit, the refrigerated ice water return pipe, the hot water return pipe, the refrigerated ice water inlet pipe, the hot water inlet pipe, the condensate output pipe, the compressed air pipe, the strain output pipe, the cleaning circuit pipe, the pure water pipe, the brewing water pipe, and the cleaning inlet pipe through the first cross-connection valve plates, the second cross-connection valve plates, the cross-connection valve plates, and the pipeline switch valves to form a strain cultivation system.
[0017] Beneficial effects
[0018] The utility model provides a strain cultivation system with automatic cleaning and steam in-situ sterilization. Compared with the prior art, it has the following beneficial effects: The strain cultivation system with automatic cleaning and steam in-situ sterilization of this application can automatically clean in-situ, perform steam in-situ sterilization, control temperature, and oxygenate, which can ensure the cleanliness of the cultivation system, pure strain cultivation, guarantee the consistency of strain cultivation, improve the cultivation efficiency, shorten the cultivation cycle, and provide technical guarantee for large-scale production to meet the production capacity required for large-scale cultivation of strains, and provide experience and templates for large-scale intelligent factories for strain cultivation. Description of the drawings
[0019] Figure 1 It is a schematic diagram of the overall principle structure of Embodiment 1 of the utility model.
[0020] Figure 2 It is a schematic diagram of the structures of the strain tank and the expansion tank.
[0021] Figure 3 It is a schematic diagram of the structure of the power assembly on the tank top.
[0022] Figure 4 It is a schematic diagram of the overall principle structure of Embodiment 2 of the utility model.
[0023] Figure 5This is the schematic diagram of the overall principle structure of Embodiment 3 of the present utility model.
[0024] In the figure: pipeline switch valves (1, 2, 3, 4, 5, 6, 22, 23, 24, 25, 26, 27, 43, 44, 45, 46, 47, 49, 65, 66, 67, 68, 69, 70, 91, 92); automatic air release valves (7, 28, 50, 71); one-way valves (8, 29, 51, 72); temperature gauges (9, 30, 52, 73); liquid level gauges (10, 31, 53, 74); sampling valves (11, 32, 54, 75); pneumatic valves (12, 33, 55, 76); flow meters (13, 34, 56, 77, 89); cone bottom switch valves (14, 35, 57, 78); branch switch valves (15, 36, 58, 79); cross-connection valves for tube sheets (16, 17, 18, 19, 20, 21, 37, 38, 39, 40, 41, 42, 59, 60, 61, 62, 63, 64, 80, 81, 82, 83, 84, 85, 93, 94, 95, 96, 97, 98, 99); overflow valve 86; return valve 87; control valve 88; return pump 90;
[0025] Strain tanks (A100, B100); expansion tanks (C100, D100); steam pipe loop 200; refrigerated ice water return pipe 300; hot water return pipe 400; refrigerated ice water inlet pipe 500; hot water inlet pipe 600; condensate output pipe 700; compressed air pipe 800; strain output pipe 900; cleaning loop pipe 1000; pure water pipe 1100; brewing water pipe 1200; cleaning inlet pipe 1300;
[0026] Top-of-tank power assembly 101; drive motor 1011; oxygen charging valve pipe 1012; cleaning valve 1013; stirring shaft 1014; cleaning ball 1015;
[0027] Strain addition port 102; culture medium addition port 103; hot water / ice water jacket 104; tank body sampling port 105; steam / condensate jacket 106; inlet / outlet port 107; tank body 108. Specific implementation manners
[0028] The following further details the present disclosure in conjunction with the accompanying drawings and implementation manners. It can be understood that the specific implementation manners described herein are only used to explain the relevant content and do not limit the present disclosure. Additionally, it should be noted that for the sake of description, only the parts related to the present disclosure are shown in the drawings.
[0029] Embodiment 1
[0030] As Figures 1 to 3As shown, an automatic cleaning and steam sterilization bacterial strain culture system comprises a bacterial strain tank A100, a cleaning system, a steam sterilization system and a bacterial strain culture system; the upper end of the tank body 108 of the bacterial strain tank A100 is provided with a tank top power component 101, a bacterial strain addition port 102 and a culture medium addition port 103; the side of the tank body 108 is provided with a hot water / ice water jacket 104, the side of the conical bottom is provided with a steam / condensate jacket 106, and the bottom is provided with an inlet / outlet 107; the side of the tank body 108 is also provided with a tank body sampling port 105;
[0031] The cleaning system pumps the cleaning liquid entering the cleaning inlet pipe 1300 into the tank body 108 through the tank washing CIP station, cleans the tank body 108 through the tank top power assembly 101, and the cleaned cleaning liquid returns from the cleaning loop pipe 1000 through the inlet / outlet port 107;
[0032] The steam sterilization system introduces high-temperature steam through the steam pipe loop 200 and inputs it into the tank 108 and the bacterial strain addition pipeline for high-temperature sterilization, and then enters the tank 108 through the compressed air pipe 800 for compressed gas cooling, and the condensed water generated by the cooling is discharged from the condensed water output pipe 700 through the steam / condensed water jacket 106;
[0033] The bacterial strain culture system introduces the pure water from the pure water pipe 1100 or the brewing water pipe 1200 into the tank body 108, and then adds the culture medium and the high temperature resistant amylase into the tank body 108 through the culture medium adding port 103. During the heating process, the stirring is always turned on in the tank; the tank body 108 is heated and raised to a temperature through the steam pipe loop 200 until the starch is fully decomposed, and the excess gas generated during the starch decomposition process is discharged from the discharge valve provided on the tank top power component 101, and the sample is taken through the sampling valve 75, and the starch decomposition is checked with iodine solution, and the tank top power component 101 continues to stir the liquid in the tank body 108; until the iodine solution detects that the starch is completely decomposed, the tank body 108 is cooled by the refrigeration ice water inlet pipe 500, the bacterial strain is connected to the tank body 108 through the bacterial strain adding port 102, the liquefaction enzyme is added, and the proliferation culture is carried out, and the sample is taken through the sampling valve 75 to detect the number of bacterial strains and the sugar content until the process requirements are met, and then the cultured bacterial strains are output and stored through the bacterial strain output pipe 900.
[0034] As a preferred embodiment, the cleaning system also includes a hot water cleaning system, which uses a water pump to introduce hot water from the hot water inlet pipe 600 into the tank body 108 through the hot water / ice water jacket 104, stirs and cleans the hot water in the tank body 108 through the tank top power assembly 101, and then returns through the hot water return pipe 400 connected to the hot water / ice water jacket 104.
[0035] As a preferred embodiment, Figure 3As shown in the figure, the top-of-tank power assembly 101 includes a drive motor 1011, an oxygen filling valve pipe 1012, a cleaning valve 1013, a stirring shaft 1014, and a cleaning ball 1015; the drive motor 1011 is fixed on the tank body 108; the stirring shaft 1014 is arranged inside the tank body 108 and is connected to the drive motor 1011; the oxygen filling valve pipe 1012 is fixed on the tank body 108; the cleaning ball 1015 is arranged inside the tank body 108 and is connected to the cleaning valve 1013 arranged on the tank body 108.
[0036] During operation: First, the cleaning liquid entering through the cleaning inlet pipe 1300 is pumped into the tank body 108 by the CIP station for tank cleaning. The top-of-tank power assembly 101 is used to clean the tank body 108. When the liquid level of the liquid level gauge 74 reaches the set value, the reflux pump 90 is started for CIP reflux. When the liquid level gauge 74 shows no liquid level, the reflux pump 90 is stopped after a 5-second delay. The stirring always runs during the cleaning process. The cleaned cleaning liquid returns through the cleaning return pipe 1000 from the inlet / outlet 107, and then this circuit is closed. Then, the hot water cleaning system pumps the hot water from the hot water inlet pipe 600 into the tank body 108 through the water pump and introduces it into the tank body 108 through the hot water / ice water jacket 104. The top-of-tank power assembly 101 continues to stir and clean the hot water in the tank body 108. After cleaning, it returns through the hot water return pipe 400 connected to the hot water / ice water jacket 104, and this circuit is closed; then the bottom cone switch valve 78 and the branch switch valve 79 are opened, and the cross-connection pipe plate connection valves (83, 84) are cross-connected and communicated. The cross-connection pipe plate connection valves (83, 84) and the control valve 88 are opened, and the pipeline switch valve 65 is opened. High-temperature steam introduced through the steam pipe circuit 200 is input into the tank body 108 and the strain addition pipeline for high-temperature sterilization. The strain culture system introduces the strain from the pure water pipe 1100 or the brewing water pipe 1200 into the tank body 108, and then adds the culture medium and thermostable amylase into the tank body 108 through the culture medium addition port 103. Then, the tank body 108 is heated and raised in temperature through the steam pipe circuit 200 until the starch is fully decomposed. Then, the tank body 108 is cooled through the refrigerated ice water inlet pipe 500. At the same time, the top-of-tank power assembly 101 stirs the liquid in the tank body 108; the strain is introduced into the tank body 108 through the strain addition port 102 for proliferation culture, and the cultured strain is output and stored through the strain output pipe 900. This culture system realizes a strain culture system that integrates cleaning and steam empty sterilization, achieves cleaning and disinfection without dead corners, can ensure the cleanliness of the culture system and pure strain culture, guarantee the consistency of strain culture, and improve the culture efficiency.
[0037] Embodiment 2
[0038] As Figures 1 to 4As shown in the figure, an expansion tank D100 is also provided beside the strain tank A100; the structure of the expansion tank D100 is the same as that of the strain tank A100, and the connection methods of the expansion tank D100 and the strain tank A100 to the steam pipe circuit 200, the refrigerated ice water return pipe 300, the hot water return pipe 400, the refrigerated ice water inlet pipe 500, the hot water inlet pipe 600, the condensate output pipe 700, the compressed air pipe 800, the strain output pipe 900, the cleaning circuit pipe 1000, the pure water pipe 1100 and the brewing water pipe 1200 are the same; cross-connection valve plates (16, 81) are respectively provided on the first cross-connection valve plates of the expansion tank D100 and the strain tank A100; the cross-connection valve plates (16, 81) connect the strain output pipes 900 of the expansion tank D100 and the strain tank A100, the cross-connection valve plates (16, 17) on the first cross-connection valve plate of the expansion tank D100 are cross-connected, and the cross-connection valve plates (80, 81) on the first cross-connection valve plate of the strain tank A100 are cross-connected. The compressed air input into the tank body 108 of the strain tank A100 through the compressed air pipe 800 transfers the strains cultured in the strain tank A100 into the tank body 108 of the expansion tank D100 for culture; a cross-connection valve plate 94 is also provided on the second cross-connection valve plate. After the cross-connection valve plates (94, 98) are cross-connected, the cleaning liquid in the cleaning inlet pipe 1300 is pumped into the tank body 108 of the expansion tank D100 for cleaning through the CIP station for tank cleaning, and the cleaning liquid is discharged through the reflux pump 90 provided on the cleaning circuit pipe 1000; the capacity of the expansion tank D100 is larger than that of the strain tank A100. The strains are proliferated and cultured in the strain tank A100, and samples are taken through the sampling valve 75 to detect the number of strains and the sugar degree value. After meeting the requirements of the strain transfer process, they are transferred to the expansion tank D100 for continuous culture, and oxygen is supplied through the oxygen filling valve pipe 1012 in the tank top power assembly 101. The flowmeter 13 records the instantaneous oxygen filling flow rate and the cumulative flow. When the temperature displayed by the thermometer 9 is higher than the process set value, the pipeline switch valves (2, 4) are opened, and the ice water in the refrigerated ice water inlet pipe 500 and the refrigerated ice water return pipe 300 is used to cool the tank until the temperature displayed by the thermometer 9 reaches the process set value, and then the pipeline switch valves (2, 4) are closed. The stirring of the tank is always turned on during the cooling process. When the temperature displayed by the culture thermometer 9 is lower than the set value, the pipeline switch valves (3, 5) are opened, and the hot water in the hot water inlet pipe 600 and the hot water return pipe 400 is used to keep the strains warm, so that the temperature displayed by the thermometer 9 is within the process set range. When the temperature reaches the process set range, the pipeline switch valves (3, 5) are closed; during the strain culture process, the exhaust pressure valve provided on the tank top automatically exhausts to ensure the pressure in the tank body 108 until the strain culture reaches the final requirements, and then it is output for use and stored for standby.This culture system realizes the functions of temperature control and oxygenation, and transfers and cultures through an expansion tank, which can ensure the cleanliness of the culture system, pure culture of strains, guarantee the consistency of strain culture, provide technical guarantee for large-scale production, improve the culture efficiency, and shorten the culture cycle.
[0039] Example 3
[0040] As Figures 1 to 5 shown, a plurality of strain tanks A100 and expansion tanks D100 are provided. For the convenience of description, taking two strain tanks (A100, B100) and two expansion tanks (C100, D100) as an example, the strain tanks (A100, B100) and the expansion tanks (C100, D100) are connected to the steam pipe loop 200, the refrigerated ice water return pipe 300, the hot water return pipe 400, the refrigerated ice water inlet pipe 500, the hot water inlet pipe 600, the condensate output pipe 700, the compressed air pipe 800, the strain output pipe 900, the cleaning loop pipe 1000, the pure water pipe 1100, the brewing water pipe 1200 and the cleaning inlet pipe 1300 through the first cross-connection pipe plate, the second cross-connection pipe plate, the cross-connection pipe plate connection valve and the pipeline switch valve to form a strain culture system.
[0041] As a preferred embodiment, the upper end of the hot water / ice water jacket 104 is communicated with the refrigerated ice water inlet pipe 500 and the hot water inlet pipe 600, and the lower end of the hot water / ice water jacket 104 is communicated with the refrigerated ice water return pipe 300 and the hot water return pipe 400; pipeline switch valves (66, 67, 68, 69, 2, 3, 4, 5, 23, 24, 25, 26, 44, 45, 46, 47) are respectively arranged on the pipelines of the refrigerated ice water inlet pipe 500, the refrigerated ice water return pipe 300, the hot water inlet pipe 600 and the hot water return pipe 400 to respectively form a refrigerated ice water loop and a hot water cleaning loop.
[0042] As a preferred embodiment, the steam pipe loop 200 includes a steam pipe connected to the tank body 108 and a steam pipe connected to the steam / condensate jacket 106; pipeline switch valves (65, 70, 1, 6, 22, 27, 43, 49) are respectively arranged on the steam pipe connected to the tank body 108 and the steam pipe connected to the steam / condensate jacket 106; the steam pipe forms a communicating pipeline with the tank body 108 and the steam / condensate jacket 106; the steam / condensate jacket 106 is communicated with the condensate output pipe 700; the condensate output pipe 700 is sequentially provided with an automatic air release valve (71, 7, 28, 50), a check valve (8, 29, 51, 72), an overflow valve 86 and a reflux valve 87 along the discharge direction.
[0043] As a preferred embodiment, a thermometer (9, 30, 52, 73) and a liquid level gauge (10, 31, 53, 74) are further provided in the tank body 108, and a sampling valve (11, 32, 54, 75) is installed in the tank body sampling port 105; the pure water pipe 1100 or the brewing water pipe 1200 introduces pure water or brewing water into the tank body 108 from the inlet / outlet port 107 through the first cross pipe plate; pipeline switch valves (91, 92) are respectively arranged on the pure water pipe 1100 or the brewing water pipe 1200 to control the opening and closing of the pure water pipe 1100 or the brewing water pipe 1200; after the cross pipe plate connection valves (83, 85) arranged on the first cross pipe plate are connected, the cone bottom switch valves (14, 35, 57, 78) and the branch switch valves (15, 36, 58, 79) on the pipeline connected to the inlet / outlet port 107 are opened, and pure water or brewing water is introduced into the tank body 108; taking Figure 1 the principle in
[0044] As a preferred embodiment, the cleaning inlet pipe 1300 is connected to the cleaning valve 1013 in the tank top power assembly 101 through a pipeline installed on the second cross pipe plate; after the cross pipe plate connection valves (97, 98) arranged on the second cross pipe plate are cross-connected and connected, the cleaning valve 1013 and the cone bottom switch valve 78 are opened, and after the cross pipe plate connection valves (80, 82) arranged on the first cross pipe plate are cross-connected and connected, the cleaning liquid in the cleaning inlet pipe 1300 is pumped into the tank body 108 through the washing tank CIP station for cleaning, and the cleaning liquid is discharged through the reflux pump 90 arranged on the cleaning return pipe 1000.
[0045] As a preferred embodiment, the compressed air pipe 800 is connected to a pipeline installed on the first cross pipe plate accessing the tank top power assembly 101; pneumatic valves (12, 33, 55, 76) and flow meters (13, 34, 56, 77) are arranged on the compressed air pipe 800. When multiple strain tanks A100 and expansion tanks D100 are provided, multiple cross pipe plate connection valves are arranged on the first cross pipe plate and the second cross pipe plate to facilitate the transfer of strains between the respective strain tanks A100 and expansion tanks D100, taking Figure 5Taking [China] as an example, the cross-header connection valves (16, 17, 18, 19, 20, 21, 37, 38, 39, 40, 41, 42, 59, 60, 61, 62, 63, 64, 80, 81, 82, 83, 84, 85, 93, 94, 95, 96, 97, 98, 99) provided on the first cross-header and the second cross-header achieve the connection of the corresponding pipelines through the connection of different cross-header connection valves on the first cross-header and the second cross-header. By setting multiple strain tanks A100 and expansion tanks D100, the production capacity required for large-scale strain cultivation is met, and experience and templates are provided for large-scale intelligent factories for strain cultivation.
[0046] Those skilled in the art should understand that the above embodiments are only for clearly illustrating the present disclosure, rather than limiting the scope of the present disclosure. For those skilled in the art, other changes or modifications can be made on the basis of the above disclosure, and these changes or modifications are still within the scope of the present disclosure.
Claims
1. An automatic cleaning and steam sterilization strain culture system, comprising a strain tank (A100), a cleaning system and a steam sterilization system; a tank top power assembly (101), a strain addition port (102) and a culture medium addition port (103) are arranged at the upper end of the tank body (108) of the strain tank (A100); a hot water / ice water jacket (104) is arranged on the side of the tank body (108), a steam / condensate jacket (106) is arranged on the side of the conical bottom, and an inlet / outlet port (107) is arranged at the bottom; a tank body sampling port (105) is further arranged on the side of the tank body (108). The cleaning system includes a tank cleaning CIP station, a cleaning inlet pipe (1300) and a cleaning return pipe (1000); the cleaning inlet pipe (1300) is connected to the tank top power assembly (101), and the cleaning return pipe (1000) is connected to the inlet / outlet port (107); the tank cleaning CIP station drives the cleaning liquid to flow in the pipelines of the cleaning inlet pipe (1300) and the cleaning return pipe (1000). The steam sterilization system includes a steam pipe circuit (200), a compressed air pipe (800) and a condensate output pipe (700); the steam pipe circuit (200) is connected to the tank body (108) and the steam / condensate jacket (106) for steam sterilization; the compressed air pipe (800) is connected to the tank body (108), and the condensate generated by cooling is discharged through the condensate output pipe (700) connected to the steam / condensate jacket (106). The inlet / outlet port (107) is respectively connected to a pure water pipe (1100) or a brewing water pipe (1200) and a strain output pipe (900).
2. The strain culture system for automatic cleaning and steam sterilization according to claim 1, wherein The cleaning system further includes a hot water cleaning system, and the hot water cleaning system includes a hot water inlet pipe (600) and a hot water return pipe (400); the hot water inlet pipe (600) and the hot water return pipe (400) are respectively connected to the hot water / ice water jacket (104) to form a hot water cleaning circuit.
3. The strain cultivation system for automatic cleaning and steam sterilization according to claim 1, wherein The tank top power assembly (101) includes a driving motor (1011), an oxygen charging valve pipe (1012), a cleaning valve (1013), a stirring shaft (1014) and a cleaning ball (1015); the driving motor (1011) is fixed on the tank body (108); the stirring shaft (1014) is arranged in the tank body (108) and is connected to the driving motor (1011); the oxygen charging valve pipe (1012) is fixed on the tank body (108); the cleaning ball (1015) is arranged in the tank body (108) and is connected to the cleaning valve (1013) arranged on the tank body (108).
4. An automatic cleaning and steam sterilization strain cultivation system according to claim 2, characterized in that, The upper end of the hot water / ice water jacket (104) is communicated with the refrigerated ice water inlet pipe (500) and the hot water inlet pipe (600), and the lower end of the hot water / ice water jacket (104) is communicated with the refrigerated ice water return pipe (300) and the hot water return pipe (400); pipeline shut-off valves (66, 68, 67, 69) are respectively arranged on the pipelines of the refrigerated ice water inlet pipe (500), the refrigerated ice water return pipe (300), the hot water inlet pipe (600), and the hot water return pipe (400) to respectively form a refrigerated ice water loop and a hot water cleaning loop.
5. The strain culture system for automatic cleaning and steam sterilization according to claim 1, characterized in that, The steam pipe loop (200) includes a steam pipe connected to the tank body (108) and a steam pipe connected to the steam / condensate jacket (106); pipeline shut-off valves (65, 70) are respectively arranged on the steam pipe connected to the tank body (108) and the steam pipe connected to the steam / condensate jacket (106); the steam pipe forms a communicating pipeline with the tank body (108) and the steam / condensate jacket (106); the steam / condensate jacket (106) is communicated with the condensate output pipe (700); an automatic air release valve (71), a check valve (72), an overflow valve (86), and a reflux valve (87) are successively arranged on the condensate output pipe (700) along the discharge direction.
6. The strain culture system for automatic cleaning and steam sterilization according to claim 1, characterized in that, A thermometer (73) and a liquid level gauge (74) are further arranged in the tank body (108), and a sampling valve (75) is installed in the tank body sampling port (105); pipeline shut-off valves (91, 92) are respectively arranged on the pure water pipe (1100) or the brewing water pipe (1200) to control the opening and closing of the pure water pipe (1100) or the brewing water pipe (1200), and are connected to the cross-connection pipe plate connection valves (83, 85) arranged on the first cross-connection pipe plate, and a cone bottom shut-off valve (78) and a branch shut-off valve (79) are arranged on the pipeline of the inlet / outlet port (107); a cross-connection pipe plate connection valve (84) is further arranged on the first cross-connection pipe plate, and a control valve (88) is arranged on the strain output pipe (900); a flow meter (89) is further arranged on the strain output pipe (900).
7. An automatic cleaning and steam sterilization strain culture system according to claim 6, characterized in that, The cleaning inlet pipe (1300) is connected to the cleaning valve (1013) in the tank top power assembly (101) through a pipeline installed on the second cross-connection pipe plate; the cross-connection pipe plate connection valves (97, 98) arranged on the second cross-connection pipe plate are cross-connected, and the cross-connection pipe plate connection valves (80, 82) arranged on the first cross-connection pipe plate are cross-connected; a reflux pump (90) is arranged on the cleaning loop pipe (1000).
8. An automatic cleaning and steam sterilization strain cultivation system according to claim 6, characterized in that, The compressed air pipe (800) is connected to a pipeline installed on the first cross-connection pipe plate accessing the tank top power assembly (101); a pneumatic valve (76) and a flow meter (77) are arranged on the compressed air pipe (800).
9. An automatic cleaning and steam sterilization strain culture system according to claim 7, characterized in that, An expansion tank (D100) is also provided beside the strain tank (A100); the structure of the expansion tank (D100) is the same as that of the strain tank (A100), and the connection methods of the expansion tank (D100) and the strain tank (A100) to the steam pipe circuit (200), the refrigerated ice water return pipe (300), the hot water return pipe (400), the refrigerated ice water inlet pipe (500), the hot water inlet pipe (600), the condensate output pipe (700), the compressed air pipe (800), the strain output pipe (900), the cleaning circuit pipe (1000), the pure water pipe (1100) and the brewing water pipe (1200) are the same; cross-connection valve plates (16, 81) are respectively provided on the second cross-connection valve plates of the expansion tank (D100) and the strain tank (A100); the cross-connection valve plates (16, 81) connect the strain output pipes (900) of the expansion tank (D100) and the strain tank (A100), the cross-connection valve plates (16, 17) on the first cross-connection valve plate of the expansion tank (D100) are cross-connected and switched on, and the cross-connection valve plates (80, 81) on the first cross-connection valve plate of the strain tank (A100) are cross-connected and switched on, so that the tank bodies (108) of the strain tank (A100) and the expansion tank (D100) are connected; a cross-connection valve plate (94) is also provided on the second cross-connection valve plate, and the cross-connection valve plates (94, 98) are cross-connected and switched on; the capacity of the expansion tank (D100) is larger than that of the strain tank (A100).
10. An automatic cleaning and steam sterilization strain cultivation system according to claim 9, characterized in that, A plurality of strain tanks (A100) and expansion tanks (D100) are provided, and the strain tanks (A100) and the expansion tanks (D100) are connected to the steam pipe circuit (200), the refrigerated ice water return pipe (300), the hot water return pipe (400), the refrigerated ice water inlet pipe (500), the hot water inlet pipe (600), the condensate output pipe (700), the compressed air pipe (800), the strain output pipe (900), the cleaning circuit pipe (1000), the pure water pipe (1100), the brewing water pipe (1200) and the cleaning inlet pipe (1300) through the first cross-connection valve plate, the second cross-connection valve plate, the cross-connection valve plate and the pipeline switch valve to form a strain cultivation system.