Cell culture dish

Abstract

The utility model provides a cell culture dish which comprises a dish bottom, a partition plate and a side wall, the partition plate and the side wall are integrated, the height of the partition plate is smaller than that of the side wall, a culture area is formed in the interior surrounded by the partition plate, and a water containing area is formed between the partition plate and the side wall; the area ratio of the water containing area to the culture area is (2-4): 1. And the interior of the whole culture dish keeps saturated humidity.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and relates to a cell culture dish suitable for a dry incubator. Background Art

[0002] In vitro embryo culture is one of the most critical links in assisted reproductive therapy, which can directly affect the clinical outcome of assisted reproductive therapy. At present, most of the incubators used clinically are dry incubators, and the culture dishes used are ordinary Petri dishes. As shown in patent CN209741187U, this kind of culture dish only has a culture area. As shown in patent CN212713572U, although this culture dish is provided with a culture area and a water storage area, it does not pay attention to how to continuously maintain the humidity of the culture environment, nor does it explore how to ensure the humidity of the culture environment under the condition of the extreme culture duration. Therefore, it is not applicable to the in vitro culture of embryos in the case of a long duration, resulting in a very narrow scope of application of the culture dish.

[0003] Moreover, in the prior art, due to splashing of water during operation or transfer, it may cause damage to cells in severe cases. However, restricted by the size of the culture dish and the use environment, this problem has not been solved. In actual operation, the operator can only operate slowly with caution, which seriously affects the operation efficiency. Summary of the Utility Model

[0004] On the one hand, in view of the problem that the scope of application of the culture dish in the prior art is narrow and cannot meet the requirements of the culture environment in actual use for a long time and in special use scenarios, the utility model provides a cell culture dish for in vitro culture of cells including germ cells. This culture dish is suitable for culturing human embryos in a dry incubator and can maintain saturated humidity under extreme culture duration conditions, greatly expanding the scope of use and use scenarios of the culture dish.

[0005] To achieve the above object, the utility model provides a cell culture dish, comprising:

[0006] A dish bottom;

[0007] A side wall, which extends upward around the edge of the dish bottom;

[0008] The space formed by the dish bottom and the side wall includes a culture area and a water storage area;

[0009] The water storage area is used to provide a humidity environment for cell culture in the culture area;

[0010] The area ratio of the water storage area to the culture area is 2 - 4:1.

[0011] Further, the cell culture dish further comprises: a partition plate disposed on the bottom of the dish, dividing the space formed by the bottom of the dish and the side wall into the culture area and the water storage area.

[0012] Further, the bottom of the dish, the side wall and the partition plate are integrally formed.

[0013] Further, the area ratio of the water storage area to the culture area is 3.24:1.

[0014] Further, the height of the partition plate is less than the height of the side wall.

[0015] Further, the ratio of the height of the side wall to the height of the partition plate is 2 - 1:1.

[0016] Further, the ratio of the height of the side wall to the height of the partition plate is 1.33:1.

[0017] Further, the height of the side wall is 10 - 14 mm, and the height of the partition plate is 7 - 12 mm.

[0018] Further, the height of the side wall is 12 mm, and the height of the partition plate is 9 mm.

[0019] Further, both the side wall and the partition plate are annular.

[0020] Further, both the side wall and the partition plate are circular ring-shaped, and the circles corresponding to the side wall and the partition plate are concentric circles.

[0021] Further, the outer diameter of the side wall is 53.54 mm.

[0022] Further, at least one culture groove is provided on the surface of the bottom of the dish in the culture area.

[0023] Further, one culture groove and two spare grooves are provided on the surface of the bottom of the dish in the culture area.

[0024] Further, the culture groove and the spare grooves are distributed in a pin shape.

[0025] Further, the culture groove and the spare grooves have the same size.

[0026] Further, the culture groove and the spare grooves are annular.

[0027] Further, the depth of the culture groove and the spare grooves is 1 mm.

[0028] Further, a number of culture micropores are provided at the bottom of the culture groove.

[0029] One of the technical effects of the technical solution of the present utility model is that by reasonably adjusting and defining the area of the water-containing area and the culture area, while ensuring the convenience of operation, the cell culture dish can always maintain an appropriate environmental humidity during the entire cycle of cell culture, greatly expanding the scope of use and application scenarios of the culture dish, and further promoting the actual mass production and popularization of cell culture dish products.

[0030] On the other hand, for the problems in the prior art that water splashing may occur during operation or transfer, which easily leads to cell contamination, and the resulting low operation efficiency. The present utility model also provides a redesigned cell culture dish; a cell culture dish, comprising:

[0031] A dish bottom;

[0032] A side wall, the side wall extending upward around the edge of the dish bottom;

[0033] A partition, the partition is arranged on the dish bottom and divides the space formed by the dish bottom and the side wall into a culture area and a water-containing area;

[0034] The water-containing area is used to provide a humidity environment for cell culture in the culture area;

[0035] A water-blocking cover, the water-blocking cover is arranged on the top of the partition.

[0036] Further, the water-blocking cover is detachably arranged on the top of the partition.

[0037] Further, the water-blocking cover is in an umbrella shape, and an opening is provided at the top of the water-blocking cover.

[0038] Further, a groove adapted to the shape of the top of the partition is provided on the bottom surface of the water-blocking cover.

[0039] Further, both the side wall and the partition are circular rings, and the circles corresponding to the side wall and the partition are concentric circles.

[0040] Further, the water-blocking cover and the partition are coaxially arranged.

[0041] Further, at least one through hole penetrating the upper and lower surfaces is provided on the water-blocking cover, and the vertical orthographic projection of the through hole is located in the water-containing area.

[0042] Further, the vertical orthographic projection of the opening of the water-blocking cover is located in the culture area, and the vertical orthographic projection of the outer edge of the water-blocking cover is located in the water-containing area.

[0043] The second technical effect of this technical solution is that by combining with the unique structure on the cell culture dish, a water baffle cover is provided and set on the partition board, breaking through the limitations of the size and usage environment of the culture dish, completely solving the problem of possible contamination in the culture area during the operation and transfer processes, and greatly improving the operation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model, the following will briefly introduce the drawings required for use in the description of the embodiments of the present utility model. The drawings in the following description are only for some embodiments. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0045] Figure 1 Shows a schematic diagram of the water storage area surrounding the culture area in an embodiment of the present application;

[0046] Figure 2 Shows a schematic diagram of a flat partition board separating the water storage area and the culture area in an embodiment of the present application;

[0047] Figure 3 Shows a schematic diagram of an arc-shaped partition board separating the water storage area and the culture area in an embodiment of the present application;

[0048] Figure 4 Shows a schematic diagram of the structure of a culture dish without a water baffle cover in an embodiment of the present application;

[0049] Figure 5 Shows a schematic diagram of the structure of a culture dish with a water baffle cover in an embodiment of the present application;

[0050] Figure 6 Shows a schematic diagram of the bottom structure of the water baffle cover in an embodiment of the present application.

[0051] BRIEF DESCRIPTION OF THE DRAWINGS: 1 - dish bottom; 2 - side wall; 3 - partition board; 4 - culture tank; 5 - water baffle cover; 6 - through hole; 7 - groove; 8 - culture micropore. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0052] The following will describe in detail the embodiments of the present utility model. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are intended to explain the present utility model, and should not be construed as a limitation to the present utility model.

[0053] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0054] In addition, the terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "a plurality of" means two or more unless otherwise specifically defined.

[0055] In the present utility model, unless otherwise clearly defined and limited, terms such as "installation", "connection", "connection", "fixation", etc. should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0056] In the present utility model, unless otherwise clearly defined and limited, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through other features therebetween. Moreover, the first feature being "above", "over" and "on" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the first feature is at a higher horizontal height than the second feature. The first feature being "below", "under" and "beneath" the second feature includes the first feature being directly below and obliquely below the second feature, or merely indicating that the first feature is at a lower horizontal height than the second feature.

[0057] In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of the disclosure of the present utility model.

[0058] In the following description, suffixes such as "module", "component", "assembly" or "unit" are only used to facilitate the description of the present utility model, and they have no specific meaning in themselves. Therefore, they can be used interchangeably.

[0059] The present utility model will be further described in detail below in conjunction with the accompanying drawings through specific embodiments.

[0060] According to an embodiment of the present utility model, a cell culture dish includes:

[0061] A dish bottom 1;

[0062] A side wall 2, the side wall 2 extending upward around the edge of the dish bottom 1;

[0063] The space formed by the dish bottom 1 and the side wall 2 includes a culture area and a water storage area;

[0064] The water storage area is used to provide a humidity environment for cell culture in the culture area;

[0065] The area ratio of the water storage area to the culture area is 2 - 4:1.

[0066] In this embodiment, a partition 3 is provided in the culture dish. The partition 3 is arranged on the dish bottom 1 and divides the space formed by the dish bottom 1 and the side wall 2 into a culture area and a water storage area. The dish bottom 1, the side wall 2 and the partition 3 are integrally formed. The material used for the cell culture dish in this embodiment is polystyrene, and other available materials such as glass can also be used in actual products. In this embodiment, the outer diameter of the side wall 2 is 53.54 mm, the wall thickness is 1 mm; the thickness of the partition is 0.8 mm, and the height of the added ultrapure water is 8 mm.

[0067] To explore the optimal ratio of the area of the water storage area to the area of the culture area, the inventor set up multiple experimental groups and control groups respectively. Among them, the independent variable is the area ratio of the culture area to the water storage area, and the dependent variable is the humidity retention time in the culture area of the culture dish. In this experiment, a dry incubator was used, the temperature of the incubator was 37 °C, and the relative humidity was 35%.

[0068] The experimental results of the experimental groups are shown in the following table:

[0069] Experimental group sequence Area ratio of water storage area to cultivation area Humidity retention duration (h) Experimental group 1 2：1 160 Experimental group 2 2.19：1 161 Experimental group 3 2.41：1 162 Experimental group 4 2.66：1 165 Experimental group 5 2.93：1 167 Experimental group 6 3.24：1 169 Experimental group 7 3.59：1 172 Experimental group 8 4：1 175 Experimental group 9 4.15：1 176 Experimental group 10 4.21：1 177 Experimental group 11 4.42：1 178

[0070] The experimental results of the control groups are shown in the following table:

[0071]

[0072]

[0073] According to the results of the above experimental group and control group, it can be seen that the humidity retention time of the culture dish is positively correlated with the area ratio of the water storage area to the culture area.

[0074] In vitro embryo culture is one of the most critical steps in assisted reproductive therapy, which can directly affect the clinical results of assisted reproductive therapy. Under the existing technology, generally, it takes 3 days (72h) for a fertilized egg to develop into a cleavage-stage embryo, and it generally takes 5 - 6 days to develop into a blastocyst. Therefore, in the solution of the present utility model, the area ratio of the water storage area to the culture area is set to be not less than 2:1, which can ensure that the humidity preservation environment is qualified for no less than 160h, completely covering the time for the fertilized egg to develop into a blastocyst in the vast majority of usage scenarios, ensuring the humidity in the culture dish during the development of the fertilized egg into a blastocyst, so that the culture dish can still maintain saturated humidity under extreme culture duration conditions, greatly expanding the usage range and usage scenarios of the culture dish.

[0075] In some embodiments, it is only necessary to satisfy the area ratio of the water storage area and the culture area, and there is no need to restrict the specific shapes of the culture area and the water storage area in the culture dish. For example Figure 1 - Figure 3 As shown, the culture area can be surrounded by the water storage area, or the culture area and the water storage area can be located on both sides of the partition 3 respectively. Of course, it is not limited to using the partition 3 to distinguish the culture area, and other reasonable separation methods can also be adopted to distinguish the water storage area and the culture area, such as setting a separated platform, a separation membrane, etc.

[0076] In addition, according to experimental research, if the area ratio of the water storage area to the culture area is further expanded, although the humidity preservation time can be further improved, if the area ratio of the water storage area is further expanded, although the humidity preservation duration can be extended, since it has exceeded the limit duration of in vitro cell culture, it is meaningless for in vitro cell culture itself, and instead it will cause the area of the culture area to be too small, making actual operation and observation inconvenient. Therefore, preferably, the area ratio of the water storage area to the culture area is set to be not greater than 4:1.

[0077] Furthermore, according to an embodiment of the present utility model, considering the longest time for the fertilized egg to develop into a blastocyst, in this embodiment, the area ratio of the water storage area to the culture area is preferably 3.24:1. Considering the convenience of operation, in this embodiment, the side wall 2 and the partition 3 are both circular rings, and the circles corresponding to the side wall 2 and the partition 3 are concentric circles. By setting the side wall 2 and the partition 3 as concentric circles, after the staff rotates the culture dish during observation, the position of the culture area does not change, avoiding the horizontal movement of the culture dish. Further, for the convenience of taking the culture dish, in this embodiment, a wavy hand-held part is also provided on the outer surface of the side wall 2.

[0078] This embodiment provides a cell culture dish, such as Figure 4As shown, the culture dish is of an integrally formed structure, including: a dish bottom 1; a side wall 2, where the side wall 2 surrounds the dish bottom 1; a partition 3, where the partition 3 is arranged on the dish bottom 1 and divides the space formed by the dish bottom 1 and the side wall 2 into a culture area and a water storage area; the area ratio of the water storage area to the culture area is 3.24:1. In this embodiment, the height of the partition 3 is less than the height of the side wall 2; the ratio of the height of the side wall 2 to the height of the partition 3 is 2 - 1:1; preferably, the height of the side wall 2 is 10 - 14 mm, and the height of the partition 3 is 7 - 12 mm.

[0079] Further, in this embodiment, the side wall 2 has a standard outer diameter of 53.54 mm and a wall thickness of 1 mm; the ratio of the height of the side wall 2 to the height of the partition 3 is 1.33:1. More specifically, the height of the side wall 2 is 12 mm, the height of the partition 3 is 9 mm, and the thickness of the partition is 0.8 mm.

[0080] According to an embodiment of the present invention, on the surface of the dish bottom 1 in the culture area, there is a culture groove and two spare grooves. In this embodiment, both the culture groove and the spare grooves are annular; the culture groove and the two spare grooves have the same size; and the culture groove and the two spare grooves are distributed in a triangular pattern. By designing the culture groove and the spare grooves in a triangular pattern, the distance between the culture groove and any one of the spare grooves is equal, which is more conducive to the staff to carry out embryo transfer, cleaning and other work.

[0081] In this embodiment, the depth of the culture groove and the spare grooves is 1 mm. At the bottom of the culture groove, there are several culture micropores 8. There are 25 culture micropores arranged in a 5*5 matrix in the culture groove. The matrix composed of all the culture micropores 8 is located in the center of the raised ring. The distance between adjacent culture micropores 8 is 0.5 mm. On the left side of the matrix, each row of the matrix is provided with a digital number, and the digital numbers are "1, 2, 3, 4, 5"; on the upper side of the matrix, each column of the matrix is provided with a letter number, and the letter numbers are "A, B, C, D, E"; based on the digital number and the letter number, each culture micropore 8 can be two-dimensionally located. For example, the first micropore in the upper left corner can be located by A1, and the first micropore in the lower right corner can be located by E5.

[0082] According to an embodiment of the present invention, such as Figure 4 - Figure 6, this embodiment provides a cell culture dish, which includes a dish bottom 1, a partition 3 and a side wall 2 integrated with the dish bottom 1. The interior surrounded by the partition 3 forms a culture area. At least one raised ring is provided on the surface of the dish bottom 1 in the culture area. A water storage area is formed between the partition 3 and the side wall 2. It also includes a water blocking cover 5 detachably arranged on the top of the partition 3. To prevent the ultrapure water in the water storage area from splashing into the culture area during the transfer of the culture dish, a water blocking cover 5 is additionally provided in this embodiment. After transferring the embryo to the culture area of the culture dish and adding ultrapure water to the water storage area, cover the top of the partition 3 with the water blocking cover 5. In this way, even if the culture dish shakes during the transfer process, the ultrapure water in the water storage area will not splash into the culture area.

[0083] Furthermore, in this embodiment, the water blocking cover 5 is in an umbrella shape and has an opening at its top. To ensure the humidity in the culture area, the culture area cannot be completely isolated from the water storage area. Therefore, an opening is provided at the top of the water blocking cover 5 in this embodiment to keep the culture area and the water storage area connected, so that the water vapor formed by the evaporation of the ultrapure water in the water storage area can cover the culture area, and at the same time it is convenient to observe the culture area.

[0084] Furthermore, in this embodiment, both the partition 3 and the side wall 2 are circular. The height of the partition 3 is less than the height of the side wall 2. The inner diameter of the water blocking cover 5 is less than the diameter of the corresponding circle of the partition 3. The outer diameter of the water blocking cover 5 is greater than the diameter of the corresponding circle of the partition 3 and less than the diameter of the corresponding circle of the side wall 2. A groove 7 adapted to the shape of the top of the partition 3 is provided on the bottom surface of the water blocking cover 5, and this groove 7 is a circular groove.

[0085] In this embodiment, the water blocking cover 5 partially covers the water storage area and partially covers the culture area. On the one hand, it can prevent the ultrapure water in the water storage area from splashing into the culture area due to the shaking of the culture dish during the transfer process. On the other hand, it can avoid the complete separation of the water storage area and the culture area, so that the ultrapure water in the water storage area cannot play the role of maintaining the humidity of the culture area.

[0086] Further, in this embodiment, the height of the side wall 2 is 12 mm, and the height of the partition 3 is 9 mm. Both the side wall 2 and the partition 3 are circular rings, and the circles corresponding to the side wall 2 and the partition 3 are concentric circles. The water retaining cover 5 is in the shape of a circular ring and is coaxially arranged with the partition 3; the horizontal height of the inner circle of the water retaining cover 5 is higher than that of the outer circle. In this embodiment, by setting the height difference between the side wall 2 and the partition 3 to 3 mm, the water retaining cover 5 will not exceed the height of the side wall 2 after being buckled on the partition 3, which is convenient for buckling the culture dish cover. In this embodiment, the water retaining cover 5 is in the shape of an umbrella with an opening at the top and is coaxially arranged with the partition 3. The vertical orthographic projection of the opening of the water retaining cover 5 is located in the culture area, and the vertical orthographic projection of the outer edge of the water retaining cover 5 is located in the water storage area. The horizontal height of the inner circle of the water retaining cover 5 is higher than that of the outer circle, so that a slope or a slope with a certain curvature is formed on the surface of the water retaining cover. The slope angle of this slope is 8-15°. When the ultrapure water in the water storage area splashes onto the surface of the water retaining cover 5, the ultrapure water can flow back into the water storage area along this slope, so as to prevent excessive loss of the ultrapure water in the water storage area caused by the transfer of the culture dish and unable to maintain the preset humidity retention time.

[0087] Further, in this embodiment, a plurality of through holes 6 penetrating the upper and lower surfaces are formed in the water retaining cover 5. The vertical orthographic projection of the through holes 6 is located in the water storage area, that is, the positions of the through holes 6 are all directly above the water storage area. The ultrapure water droplets splashing onto the upper surface of the water retaining cover 5 will flow towards the edge of the water retaining cover 5 under the action of gravity. After hitting the through holes 6 penetrating the upper and lower surfaces, the water droplets will flow back into the water storage area through the through holes 6. By forming a plurality of through holes 6 penetrating the upper and lower surfaces in the water retaining cover 5, the flow path of the ultrapure water droplets splashing onto the water retaining cover 5 is reduced, so as to minimize the loss of ultrapure water.

[0088] In the description of the specification, the description with reference to terms such as "one embodiment", "some embodiments", "one example", "some examples", "a specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

Claims

1. A cell culture dish, comprising: A dish bottom; A side wall that extends upwardly around the edge of the dish bottom; The space formed by the dish bottom and the side wall includes a culture area and a water storage area; The water storage area is used to provide a humidity environment for cell culture in the culture area; Characterized in that the area ratio of the water storage area to the culture area is 2 - 4:

1.

2. The cell culture dish according to claim 1, characterized in that, Comprising: A partition that is disposed on the dish bottom and divides the space formed by the dish bottom and the side wall into the culture area and the water storage area.

3. The cell culture dish according to claim 2, characterized in that, The dish bottom, the side wall and the partition are integrally formed.

4. A cell culture dish according to claim 1, wherein, The area ratio of the water storage area to the culture area is 3.24:

1.

5. The cell culture dish according to claim 2, characterized in that, The height of the partition is less than the height of the side wall.

6. The cell culture dish according to claim 2, wherein The ratio of the height of the side wall to the height of the partition is 2 - 1:

1.

7. A cell culture dish according to claim 6, characterized in that, The ratio of the height of the side wall to the height of the partition is 1.33:

1.

8. A cell culture dish according to claim 5, characterized in that, The height of the side wall is 10 - 14 mm, and the height of the partition is 7 - 12 mm.

9. A cell culture dish according to claim 8, wherein, The height of the side wall is 12 mm, and the height of the partition is 9 mm.

10. A cell culture dish according to claim 2, characterized in that, The side wall and the partition are both annular.

11. A cell culture dish according to claim 10, characterized in that, The side wall and the partition are both circular ring-shaped, and the circles corresponding to the side wall and the partition are concentric circles.

12. A cell culture dish according to claim 11, wherein, The outer diameter of the side wall is 53.54 mm.

13. A cell culture dish according to claim 1, characterized in that, At least one culture groove is provided on the surface of the dish bottom in the culture area.

14. A cell culture dish according to claim 13, characterized in that, One culture groove and two spare grooves are provided on the surface of the dish bottom in the culture area.

15. A cell culture dish according to claim 14, characterized in that, The culture groove and the spare grooves are distributed in a pin shape.

16. A cell culture dish according to claim 14, characterized in that, The culture groove and the spare grooves have the same size.

17. A cell culture dish according to claim 14, characterized in that, The culture groove and the spare grooves are annular.

18. A cell culture dish according to claim 14, characterized in that, The depth of the culture groove and the spare grooves is 1 mm.

19. A cell culture dish according to claim 13, characterized in that, A number of culture micropores are provided at the bottom of the culture groove.

20. A cell culture dish, characterized in that, Comprising: A dish bottom; A side wall that extends upwardly around the edge of the dish bottom; A partition that is disposed on the dish bottom and divides the space formed by the dish bottom and the side wall into a culture area and a water storage area; The water storage area is used to provide a humidity environment for cell culture in the culture area; A water retaining cover that is disposed on the top of the partition.

21. A cell culture dish according to claim 20, wherein, The water retaining cover is detachably disposed on the top of the partition.

22. A cell culture dish according to claim 20, wherein, The water retaining cover is in an umbrella shape, and an opening is provided at the top of the water retaining cover.

23. A cell culture dish according to claim 20, characterized in that, A groove adapted to the shape of the top of the partition is provided on the bottom surface of the water retaining cover.

24. A cell culture dish according to claim 20, characterized in that, The side wall and the partition are both circular ring-shaped, and the circles corresponding to the side wall and the partition are concentric circles.

25. A cell culture dish according to claim 24, characterized in that, The water retaining cover and the partition are coaxially arranged.

26. A cell culture dish according to claim 20, characterized in that, At least one through hole penetrating the upper and lower surfaces is provided on the water retaining cover, and the vertical orthographic projection of the through hole is located in the water storage area.

27. A cell culture dish according to claim 20, characterized in that, The vertical orthographic projection of the opening of the water retaining cover is located in the culture area, and the vertical orthographic projection of the outer edge of the water retaining cover is located in the water storage area.

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