Device for staining specimen smear

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Through the design of hollow skeletons and elastic sheets, the high cost and drop problems of the smear dyeing device are solved, and diversified dyeing and stable fixation are achieved. It is suitable for a variety of dyeing types, reducing operational difficulty and space requirements.

CN223139133UActive Publication Date: 2025-07-22SHANXI PROVINCIAL PEOPLES HOSPITAL (AFFILIATED HOSPITAL OF SHANXI HEALTH VOCATIONAL COLLEGE)

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Patent Information

Application Number: CN202422281947.6
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-09-19
Publication Date: 2025-07-22
Estimated Expiration: 2034-09-19

AI Technical Summary

Technical Problem

The existing smear dyeing devices are expensive, take up a large space, and have a single dyeing type. They cannot meet the diverse needs of clinical microbial specimen smears, and they are likely to cause smears to fall during the transfer process, affecting the test results.

Method used

The glass slide is fixed by hollow skeleton and elastic sheet. The combination of elastic plate and screws is used to achieve stable fixation of the slide. It is suitable for a variety of staining types and operates on the staining pool to reduce the risk of smear falling.

Benefits of technology

It realizes stable fixation of slides, reduces the risk of smear fall, is suitable for a variety of staining types, is cheap, is suitable for various hospitals, and reduces manual operation pressure and space occupation.

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Abstract

The utility model relates to the technical field of microbiological detection, and discloses a device for staining a specimen smear, a plurality of elastic sheets are fixed on a hollow framework at intervals, each elastic sheet comprises a lower fixing plate, a connecting plate and an elastic pressing plate which are integrally connected, one side of the lower fixing plate is fixed with the connecting plate, the connecting plate is vertically arranged, and the elastic pressing plate is fixedly connected with the lower fixing plate. The upper edge of the connecting plate is fixed to an elastic pressing plate, a screw is installed at the position, right facing the lower fixing plate, of the elastic pressing plate, the screw sequentially penetrates through the elastic pressing plate and the lower fixing plate to be fixed to the hollow framework, the free end of the elastic pressing plate is bent downwards and curled inwards, and the folding portion of the elastic pressing plate abuts against the upper surface of the hollow framework. According to the device, batch treatment and stable placement of glass slides are realized, dye liquor can be effectively saved, smears are not easy to fall off due to touch, and process operation is easy; the raw materials of the device are simple and easy to obtain, the price is low, and the adaptability is high.

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Description

Technical Field

[0001] The utility model relates to the technical field of microbial detection, in particular to a smear inspection device, specifically a device for specimen smear staining. Background Art

[0002] In medical examinations, the morphological examination of microorganisms is an important means for clinical pathogen diagnosis. Before microscopic examination, many specimens such as blood, urine, sputum, etc. need to be smeared, stained, and then observed.

[0003] Most of the existing commercial staining devices are developed for pathological section staining. Even if there are individual instruments that can stain microbial specimen smears, although they can achieve automation and high throughput of smear staining, due to their high price, need to occupy a fixed space, single staining type, high maintenance and upkeep costs, etc., they are not suitable for use in primary hospitals; for large and medium-sized hospitals, automated instruments can be used to save labor costs and time costs for staining a large number of smears of a single type, but some special staining types and staining types that are not easy to automate with instruments still require manual staining. Therefore, a staining device with a low price, convenient operation, flexibility, and the ability to handle various staining types is essential for all hospitals. Because the staining methods for pathological sections are relatively fixed and limited, most of the existing manual staining devices on the market are developed for pathological sections, which cannot meet the diverse staining needs of clinical microbial specimen smears, and there is almost no device specifically for clinical microbial specimen smear staining. The R & D personnel are in the front line of clinical microbial inspection work and have more than ten years of work experience in this field. They are familiar with various staining methods required in this field. Based on their own years of work practice and combined with the beneficial experience of predecessors, this device is specially developed to fill the gap in this field.

[0004] During operation, a clean glass slide needs to be taken, marked with a marker pen, and one side edge of the slide is held for operation. During the operation process, the specimen needs to be placed on the glass slide, and then a small dropper is used to add dye to perform dropwise staining on the specimen on the slide. The stained glass slide needs to be placed on a staining rack and left to stand for a period of time. After successful dropwise staining, the specimen is moved to the microscope for observation; during a large number of operations, when manually holding and operating, it is easy to occur during the transfer process of the glass slide. Due to the small holding area and the thin thickness of the glass slide, during the process of taking and placing the glass slide, it is extremely easy for the smear to fall due to being touched, resulting in the failure of specimen smear staining.

[0005] There is little research on the smear staining device for clinical microbial test specimens, and there is no commercial standardization. Most of the existing devices are self-made in the laboratory and have no fixed style, and the materials used are also diverse. During the transfer process, it is easy to cause contamination or falling. Summary of the Invention

[0006] The present utility model aims to solve the problem that the current smear staining device cannot meet the requirements of a large number of experimental operations. During the transfer process, the dye is prone to fall due to being touched, resulting in the failure of specimen smear staining and affecting the normal progress of the inspection. A device for specimen smear staining is provided.

[0007] The present utility model is realized by adopting the following technical solutions:

[0008] A device for specimen smear staining includes a hollow skeleton. A number of elastic sheets are fixedly spaced on the hollow skeleton. Each elastic sheet includes a lower fixing plate, a connecting plate, and an elastic pressing plate that are integrally connected. One side of the lower fixing plate is fixed to the connecting plate. The connecting plate is arranged vertically. The upper edge of the connecting plate is fixed to the elastic pressing plate. A screw is installed at the position where the elastic pressing plate faces the lower fixing plate. The screw sequentially passes through the elastic pressing plate and the lower fixing plate and is fixed on the hollow skeleton. The free end of the elastic pressing plate bends downward and curls inward. The folded part of the elastic pressing plate abuts against the upper surface of the hollow skeleton.

[0009] During implementation, it includes a hollow skeleton. The hollow skeleton is made of a metal hollow flat square tube, which has the characteristics of corrosion resistance, wear resistance, and heat resistance, and can be applicable to both hot staining and cold staining at the same time. The length of the hollow skeleton is greater than the width of the staining water tank, which is convenient for placing the device on the staining water tank. A number of elastic sheets are fixedly spaced on the hollow skeleton. There are 5 - 10 elastic sheets on the hollow skeleton for fixing the glass slide. The elastic sheets are made of metal sheets. Each elastic sheet includes a lower fixing plate, a connecting plate, and an elastic pressing plate that are integrally connected. One side of the lower fixing plate is fixed to the connecting plate. The connecting plate is arranged vertically. The upper edge of the connecting plate is fixed to the elastic pressing plate. A screw is installed at the position where the elastic pressing plate faces the lower fixing plate. The screw sequentially passes through the elastic pressing plate and the lower fixing plate and is fixed on the hollow skeleton. The free end of the elastic pressing plate bends downward and curls inward. The folded part of the elastic pressing plate abuts against the upper surface of the hollow skeleton; a glass slide is inserted between the folded part of the elastic pressing plate and the upper surface of the hollow skeleton. The frosted end of the glass slide is tightly inserted between the elastic pressing plate and the hollow skeleton. The free end of the glass slide is suspended outside the hollow skeleton; the widths of the lower fixing plate, the connecting plate, and the elastic pressing plate are the same and greater than the width of the glass slide.

[0010] As a supplement, when the width of the staining water tank is very large, if the length of the hollow skeleton is too long, it is prone to inconvenient operation. At this time, the length of the hollow skeleton is less than the width of the staining water tank, and an extension skeleton is slidably installed on the bottom surface of the hollow skeleton; specifically, slide rail mounting plates are respectively installed on both sides of the bottom surface at both ends of the hollow skeleton, and an extension skeleton is installed between the two slide rail mounting plates at the same end through a slide rail; as another way, the length of the hollow skeleton is less than the width of the staining water tank, and completely identical extension skeletons are respectively inserted at both ends of the hollow skeleton, and a fixing knob is installed on the side wall of the hollow skeleton.

[0011] In use, place the integrally connected lower fixing plate, connecting plate, and elastic pressing plate on the hollow skeleton according to the number of experiments required, align the threaded holes on the hollow skeleton with the threaded holes on the lower fixing plate, and screw a screw downward from one end of the elastic pressing plate, passing through the lower fixing plate and finally through the hollow skeleton to firmly fix the elastic sheet on the hollow skeleton. Gently lift the free end of the elastic pressing plate and adjust the tightness between the folding part of the elastic pressing plate and the hollow skeleton so that it can stably hold a glass slide. If it is too loose, tighten the screw to prevent the glass slide from being unstable after insertion. If it is too tight, tighten the screw to avoid difficulty in inserting the glass slide or crushing the glass slide due to excessive pressure.

[0012] Insert the frosted end of the glass slide into the gap between the folding part of the elastic pressing plate and the upper surface of the hollow skeleton, so that the glass slide is stably clamped between the free end of the elastic pressing plate and the hollow skeleton. The free end of the glass slide extends out of the elastic pressing plate and is arranged in suspension. After fixing the glass slide, the experiment can be started.

[0013] When cleaning is required, directly place the hollow skeleton on the staining water tank for easy operation.

[0014] Compared with the prior art, the present application has the following beneficial effects:

[0015] The device for specimen smear staining provided by the present utility model realizes the stable fixation of the glass slide by using an elastic sheet, enables batch processing, reduces the number of times of taking each glass slide, and can be stably placed during the experiment, effectively saving the staining solution. The smear is not easily dropped due to touching and is easy to operate in a process flow. Moreover, the raw materials of this device are simple and easy to obtain, the price is low, the adaptability is strong, it can be used for various types of specimen smear staining, and can also be used for heat staining of smears (such as Ziehl-Neelsen staining). This device can be effectively applied to the synchronous fixation of multiple specimen smears, Gram staining operation of multiple specimen smears, fluorescence staining operation of multiple specimen smears, and acid-fast staining operation (including heat staining and cold staining), etc.

[0016] Compared with commercial staining instruments, the present utility model has great price advantages and application flexibility, can be applied to various staining types, alleviates the pressure of manual smear staining, and this device is flexible in use, does not occupy extra space, has no special requirements for the environmental area of the laboratory, and is suitable for popularization and use in all hospitals. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of Embodiment 1.

[0018] Figure 2 It is a schematic structural diagram of Embodiment 1 in the use state.

[0019] Figure 3 It is a top view of Embodiment 1.

[0020] Figure 4 Side view of the usage state of Example 1.

[0021] Figure 5 Schematic structural view of the glass slide.

[0022] Figure 6 Effect diagram when Example 1 is placed in the staining water tank.

[0023] Figure 7 Front view schematic of Example 2.

[0024] Figure 8 Schematic structural view of Example 3.

[0025] In the figure: 1 - hollow skeleton; 2 - elastic sheet; 201 - lower fixing plate; 202 - connecting plate; 203 - elastic pressing plate; 3 - screw; 4 - staining water tank; 5 - glass slide; 6 - lengthened skeleton; 7 - slide rail mounting plate; 8 - slide rail; 9 - fixing knob. Specific implementation mode

[0026] The following combines specific embodiments to describe the present utility model. Example 1

[0027] A device for staining specimen smears, as Figures 1 to 4 shown: It includes a hollow skeleton 1, which is made of a metal hollow flat square tube, has the characteristics of corrosion resistance, wear resistance, and heat resistance, and can be applicable to both hot staining and cold staining at the same time. The length of the hollow skeleton 1 is greater than the width of the staining water tank 4, which is convenient for placing the device on the staining water tank 4. A plurality of elastic sheets 2 are fixedly arranged at intervals on the hollow skeleton 1. There are 5 to 10 elastic sheets 2 on the hollow skeleton 1 for fixing the glass slide 5. The elastic sheets 2 are made of metal sheets. Each elastic sheet 2 includes an integrally connected lower fixing plate 201, a connecting plate 202, and an elastic pressing plate 203. One side of the lower fixing plate 201 is fixed to the connecting plate 202. The connecting plate 202 is arranged vertically. The upper edge of the connecting plate 202 is fixed to the elastic pressing plate 203. A screw 3 is installed at the position where the elastic pressing plate 203 faces the lower fixing plate 201. The screw 3 sequentially passes through the elastic pressing plate 203 and the lower fixing plate 201 and is fixed on the hollow skeleton 1. The free end of the elastic pressing plate 203 bends downward and curls inward. The folded part of the elastic pressing plate 203 abuts against the upper surface of the hollow skeleton 1; A glass slide 5 is inserted between the folded part of the elastic pressing plate 203 and the upper surface of the hollow skeleton 1, as Figure 4 , 5 shown: The frosted end of the glass slide 5 is tightly inserted between the elastic pressing plate 203 and the hollow skeleton 1, and the free end of the glass slide 5 is suspended outside the hollow skeleton 1; The lower fixing plate 201, the connecting plate 202, and the elastic pressing plate 203 have the same width and are greater than the width of the glass slide 5.

[0028] During use, place the integrally connected lower fixing plate 201, connecting plate 202, and elastic pressing plate 203 on the hollow skeleton 1 according to the number of experiments required, align the threaded holes on the hollow skeleton 1 with the threaded holes on the lower fixing plate 201, and screw the screw 3 downward from one end of the elastic pressing plate 203, passing through the lower fixing plate 201 and finally through the hollow skeleton 1 to firmly fix the elastic sheet 2 on the hollow skeleton 1. Gently lift the free end of the elastic pressing plate and adjust the tightness between the folded part of the elastic pressing plate 203 and the hollow skeleton 1 so that it can stably hold a glass slide. If it is too loose, tighten the screw to prevent the glass slide 5 from being unstable after insertion; if it is too tight, loosen the screw to avoid difficulty in inserting the glass slide 5 or crushing the glass slide due to excessive pressure.

[0029] Insert the frosted end of the glass slide 5 into the gap between the folded part of the elastic pressing plate 203 and the upper surface of the hollow skeleton 1 so that the glass slide 5 is stably clamped between the free end of the elastic pressing plate 203 and the hollow skeleton 1, and the free end of the glass slide 5 extends out of the elastic pressing plate 203 and is suspended. After fixing the glass slide 5, the experiment can be started.

[0030] As Figure 6 shown: When cleaning is required, directly place the hollow skeleton 1 on the staining water tank 4 for easy operation. Embodiment 2

[0031] As Figure 7 shown: When the width of the staining water tank is very large, if the length of the hollow skeleton is too long, it is likely to cause inconvenient operation. At this time, the length of the hollow skeleton 1 is less than the width of the staining water tank 4, and an extension skeleton 6 is slidably installed on the bottom surface of the hollow skeleton 1; specifically, slide rail mounting plates 7 are respectively installed on both sides of the bottom surfaces at both ends of the hollow skeleton 1, and an extension skeleton 6 is installed between the two slide rail mounting plates 7 at the same end through a slide rail 8.

[0032] During use, pull out the extension skeleton 6 from both ends of the hollow skeleton 1 and place the two ends of the extension skeleton 6 on both sides of the staining water tank 4 to achieve stable placement. Embodiment 3

[0033] As Figure 8 shown: The length of the hollow skeleton 1 is less than the width of the staining water tank 4, and exactly the same extension skeletons 6 are respectively inserted at both ends of the hollow skeleton 1, and a fixing knob 9 is installed on the side wall of the hollow skeleton 1.

[0034] During use, pull out the extension skeletons 6 from both ends of the hollow skeleton 1 to an appropriate position and tighten the fixing knob 9 to fix the extension skeletons 6.

[0035] The scope of protection claimed by the present utility model is not limited to the above specific embodiments. Moreover, for those skilled in the art, the present utility model can have various deformations and modifications. Any modification, improvement, and equivalent replacement made within the concept and principle of the present utility model should be included within the scope of protection of the present utility model.

Claims

1. A device for specimen smear staining, characterized in that: It includes a hollow skeleton (1), and a number of elastic sheets (2) are fixedly spaced on the upper part of the hollow skeleton (1). Each elastic sheet (2) includes a lower fixing plate (201), a connecting plate (202), and an elastic pressing plate (203) which are integrally connected. One side of the lower fixing plate (201) is fixed to the connecting plate (202). The connecting plate (202) is arranged vertically. The upper edge of the connecting plate (202) is fixed to the elastic pressing plate (203). A screw (3) is installed at the position where the elastic pressing plate (203) faces the lower fixing plate (201). The screw (3) sequentially passes through the elastic pressing plate (203) and the lower fixing plate (201) and is fixed on the hollow skeleton (1). The free end of the elastic pressing plate (203) is bent downward and rolled inward. The folded part of the elastic pressing plate (203) abuts against the upper surface of the hollow skeleton (1).

2. The device for specimen smear staining according to claim 1, wherein: A glass slide (5) is inserted between the folded part of the elastic pressing plate (203) and the upper surface of the hollow skeleton (1). The frosted end of the glass slide (5) is tightly inserted between the elastic pressing plate (203) and the hollow skeleton (1). The free end of the glass slide (5) is suspended outside the hollow skeleton (1).

3. The device for specimen smear staining according to claim 1, wherein: The hollow skeleton (1) is made of a metal hollow flat square tube; the elastic sheet (2) is made of a metal sheet.

4. A device for specimen smear staining according to claim 1, characterized in that: There are 5 - 10 elastic sheets (2) provided on the hollow skeleton (1).

5. The device for specimen smear staining according to claim 1, characterized in that: The lower fixing plate (201), the connecting plate (202), and the elastic pressing plate (203) have the same width, and the width is smaller than the width of the glass slide (5).

6. The device for specimen smear staining according to claim 1, wherein: The length of the hollow skeleton (1) is greater than the width of the staining water tank (4).

7. The device for specimen smear staining according to claim 1, characterized in that: The length of the hollow skeleton (1) is smaller than the width of the staining water tank (4). A lengthening skeleton (6) is slidably installed on the bottom surface of the hollow skeleton (1).

8. A device for specimen smear staining according to claim 7, characterized in that: Sliding rail mounting plates (7) are respectively installed on both sides of the bottom surface at both ends of the hollow skeleton (1). A lengthening skeleton (6) is installed between the two sliding rail mounting plates (7) at the same end through a sliding rail (8).

9. The device for specimen smear staining according to claim 1, wherein: The length of the hollow skeleton (1) is smaller than the width of the staining water tank (4). Completely identical lengthening skeletons (6) are respectively inserted at both ends of the hollow skeleton (1). A fixing knob (9) is installed on the side wall of the hollow skeleton (1).