Vacuum box suitable for plant vacuum infiltration transient expression and vacuum infiltration equipment

Abstract

The utility model discloses a vacuum box suitable for plant vacuum infiltration transient expression and vacuum infiltration equipment, the vacuum box suitable for plant vacuum infiltration transient expression comprises a vacuum pump, a box cabinet, an integral type storage rack and a water tank, the vacuum pump is connected with the box cabinet, the box cabinet is provided with a cabinet body and a cabinet door, the cabinet door is fixed with the cabinet body through a hasp, and the integral type storage rack is connected with the water tank. A sealing strip is arranged on the cabinet body, so that the cabinet body is in sealed connection with the cabinet door when the cabinet door is closed; the integral commodity shelf comprises a supporting frame and a plurality of partition plates, the supporting frame is of a frame structure formed by splicing rod pieces, the horizontal partition plates are installed on the supporting frame from top to bottom so that the supporting frame can be divided into multiple layers, and the integral commodity shelf is placed in the cabinet body; one water tank is placed on each partition plate; and for each water tank, the whole water tank is rectangular and is provided with a plurality of notches, and the notches are arranged in an array. According to the device, plant leaves can be conveniently put into bacterial liquid in the water tank to be infiltrated, the treatment scale is large, and the infiltration efficiency is high.

Description

Technical Field

[0001] The utility model belongs to the field of vacuum equipment, and more specifically, relates to a vacuum chamber and a vacuum infiltration device suitable for transient expression of plant vacuum infiltration. Background Art

[0002] Transient expression technology is a technology that transfers a target gene into target cells within a relatively short time, establishes a temporary and highly efficient expression system in the cells, and obtains a transient high-level expression of the target gene. Compared with stable expression, transient expression takes a short time and does not require the integration of foreign genes into the host plant chromosome, and is more suitable for some cell biology studies of gene-protein. In recent years, transient expression has been applied to the research and application development of various plants at home and abroad. Transient expression systems have been established for crops such as wheat, corn, and rice, horticultural ornamental plants such as petunia, catharanthus roseus, and cattail, and algae plants such as green algae, providing convenience for their gene function research and utilization.

[0003] Agrobacterium-mediated transient expression is a rapid and effective method for analyzing gene expression. Its principle is to insert the target gene into a vector, transform it into Agrobacterium tumefaciens, and use methods such as vacuum infiltration transfection or injection to make Agrobacterium tumefaciens contact with plant material cells, so that most of the T-DNA enters the nucleus for transient expression, and the expression of foreign genes can be detected after a few days.

[0004] In the existing vacuum infiltration technology, for small-scale vacuum infiltration in the laboratory, a glove box is used for infiltration, with only two plants per infiltration, a small quantity, and low processing efficiency, which is not conducive to large-scale infiltration of plants.

[0005] In some existing vacuum chambers, there is a tray inside the chamber. Generally, after opening the door of the vacuum chamber, a single person operates in front of the vacuum chamber door and places the object on the tray (the other three sides of the vacuum chamber are blocked by cabinet boards and cannot be operated). Single-person operation is time-consuming and laborious, and the position of the tray in the vacuum chamber is generally fixed, and the position of the tray cannot be adjusted and the quantity cannot be expanded, thus limiting the scale of vacuum treatment.

[0006] In addition, during vacuum infiltration, planting pots are generally used independently. When plants are planted in individual planting pots and vacuum infiltration is carried out on plants using plant transient expression technology, the single pots of plants need to be individually carried into a glove box or a vacuum chamber one by one, with low handling efficiency.

[0007] If multiple planting pots are carried into a larger vacuum chamber respectively, carrying them one by one in batches is rather cumbersome. Summary of the Utility Model

[0008] In view of the above deficiencies or improvement requirements of the prior art, the present utility model provides a vacuum chamber and a vacuum infiltration device suitable for transient expression of plant vacuum infiltration. An integral storage rack is used to hold the water tank, and the water tank is provided with notches arranged in an array, which can perform large-scale vacuum infiltration on plants, thereby improving production efficiency.

[0009] To achieve the above object, according to one aspect of the present utility model, there is provided a vacuum chamber suitable for transient expression of plant vacuum infiltration, which is characterized in that it includes a vacuum pump, a cabinet, an integral storage rack and a water tank, wherein:

[0010] The vacuum pump is connected to the cabinet for evacuating the inside of the cabinet.

[0011] The cabinet has a cabinet body and a cabinet door. The cabinet door is rotatably mounted on the cabinet body through a vertical hinge to facilitate the integral storage rack to enter and exit the cabinet. The cabinet door is fixed to the cabinet body through a buckle, and a sealing strip is provided on the cabinet body to make the cabinet body and the cabinet door be hermetically connected when the cabinet door is closed.

[0012] The integral storage rack includes a support frame and multiple partition boards. The support frame is a frame structure spliced by rods, and multiple horizontal partition boards are installed on the support frame from top to bottom, thereby dividing the support frame into multiple layers. The integral storage rack is placed inside the cabinet body.

[0013] Each partition board is respectively provided with a water tank.

[0014] For each water tank, it is integrally rectangular, has multiple notches and these notches are arranged in an array.

[0015] Preferably, a negative pressure gauge is connected to the cabinet body of the cabinet for obtaining the negative pressure value inside the cabinet.

[0016] Preferably, the bottom of the integral storage rack has a space for accommodating the forks of a forklift, so that the forklift can send the integral storage rack into and out of the cabinet body.

[0017] Preferably, the number of the vacuum pumps is two. Two vacuum pump interfaces are provided on the cabinet body of the cabinet, and each vacuum pump interface is respectively connected to a vacuum pump.

[0018] Preferably, the vacuum pump is a rotary vane vacuum pump, and the vacuum pump has an oil mist filter.

[0019] Preferably, a drain hole and a drain valve are provided at the bottom of the cabinet body of the cabinet, and the drain hole is connected to the drain valve.

[0020] According to another aspect of the present utility model, there is also provided a vacuum infiltration device applicable to transient expression of plants by vacuum infiltration, including the vacuum chamber described above, characterized in that it further includes a planting device, and the planting device includes a planting pot assembly, a cover plate and a buckle, wherein:

[0021] The planting pot assembly includes a planting pot mounting plate and a plurality of planting pots, and the planting pot mounting plate is integrally formed with all the planting pots;

[0022] For each of the planting pots, it has an integrally formed pot body and a bottom plate. The top of the pot body is fixed to the planting pot mounting plate, and a collecting groove and a plurality of grooves arranged circumferentially are provided on the bottom surface of the bottom plate. Each of the grooves is respectively communicated with the collecting groove, and a plurality of first through holes are further provided on the bottom plate;

[0023] At least one of the first through holes is provided between any two adjacent grooves;

[0024] The planting pot mounting plate is respectively provided with second through holes at positions corresponding to each of the planting pots, and each of the second through holes is respectively communicated with the inner cavity of a planting pot;

[0025] The cover plate covers the planting pot mounting plate, and the cover plate is respectively provided with third through holes at positions corresponding to each of the second through holes. Each of the third through holes is respectively communicated with a second through hole, and the aperture of the third through hole is smaller than the aperture of the second through hole. The cover plate and the planting pot mounting plate are fixedly connected together through a plurality of the buckles, so as to place the cover plate and the planting pot assembly together on the water tank, so as to place the cover plate on the water tank to immerse the plant leaves in the bacterial liquid in the tank opening for infiltration.

[0026] Preferably, each of the buckles is respectively hinged to the planting pot through a horizontal hinge shaft.

[0027] Preferably, hinge seats are provided on the pot bodies of each of the planting pots, and each of the hinge shafts is respectively connected to the hinge seats of two adjacent planting pots.

[0028] Preferably, the edge of the top surface of the cover plate has a notch groove;

[0029] The buckle for fixedly connecting the cover plate and the planting pot mounting plate extends into the notch groove, and the top surface of the buckle is flush with the top surface of the cover plate.

[0030] Generally speaking, compared with the prior art by the above technical solutions conceived by the present utility model, the following beneficial effects can be achieved:

[0031] 1) The vacuum box of the present utility model uses an integral storage rack and the partition board above to support the water tank. Moreover, the integral storage rack is divided into multiple layers from top to bottom by a plurality of horizontal partition boards, and the water tanks placed on each partition board can also be divided into upper and lower multiple layers, thereby facilitating the immersion of plant leaves into the bacterial liquid in the notch of the water tank. Moreover, the notches are arranged in an array on the water tank, which is convenient for large-scale vacuum infiltration of plants and improves production efficiency.

[0032] 2) The vacuum box of the present utility model uses an integral storage rack, which is convenient for multiple people to place water tanks and planting devices on the integral storage rack at the same time, improving work efficiency. Then, the integral storage rack can be integrally moved into the cabinet body of the vacuum box, thereby improving handling efficiency.

[0033] 3) The vacuum box of the present utility model uses an integral storage rack, and the number of partition boards can be conveniently set on the support frame, and the required number of layers can be separated according to needs, which is convenient for expanding the number of layers of the partition boards, so that more water tanks can be placed, and the infiltration quantity and production efficiency are improved.

[0034] 4) The vacuum infiltration device of the present utility model suitable for plant vacuum infiltration transient expression has a plurality of planting pots in the planting pot assembly, and they are all installed on the planting pot mounting plate. Plants that need to be subjected to vacuum infiltration using the plant transient expression technology are planted in all the planting pots. After the plant leaves grow to a certain extent, the entire planting device is transported as a whole and the entire planting device is turned over and inverted into the water tank with bacterial liquid, so that the plant leaves are immersed in the bacterial liquid. Then, the planting device and the water tank are placed in the vacuum box together, and all the plants in all the planting pots of the planting device can be subjected to vacuum infiltration treatment at one time. The treatment scale and quantity are large and the efficiency is high. Compared with traditional small-pot planting, the present utility model can concentrate planting, centralized seedling transplantation, centralized handling, and centralized infiltration, improving production efficiency.

[0035] 5) The vacuum infiltration device of the present utility model suitable for plant vacuum infiltration transient expression uses a plurality of buckles to connect the cover plate and the planting pot mounting plate together. The connection is reliable. Through the connection of the buckles, the planting device can be integrally turned over and the leaves can be placed in the bacterial liquid, improving stability. Moreover, compared with the method of sequentially placing the plants in single planting pots, it saves labor and greatly improves efficiency. At the same time, since they are placed together, the damage to the plants is reduced, the damage and dropping of the leaves can be greatly avoided, the loss is reduced, and the risk of plant wound infection is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 is an exploded schematic view of the vacuum box in the present utility model;

[0037] Figure 2 、 Figure 3 are schematic views of the planting device in the present utility model from different perspectives;

[0038] Figure 4 This is a schematic diagram of the snap fastener installed on the planting pot assembly in the present utility model. Detailed implementation manners

[0039] In order to make the objectives, technical solutions and advantages of the present utility model clearer and more understandable, the present utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model. In addition, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0040] Refer to Figure 1 , a vacuum chamber applicable to transient expression of plant vacuum infiltration, includes a vacuum pump (not shown in the figure), a cabinet 7, an integral storage rack 8 and a water tank 9, wherein:

[0041] The vacuum pump is connected to the cabinet 7 to evacuate the interior of the cabinet 7 so that the vacuum degree (negative pressure value) in the cabinet 7 reaches a set value. The vacuum chamber can support two vacuum pumps to work simultaneously to evacuate the cabinet 7, greatly improving the vacuum efficiency and reducing the vacuum evacuation time.

[0042] The cabinet 7 has a cabinet body 71 and a cabinet door 72. The cabinet door 72 is rotatably installed on the cabinet body 71 through a vertical hinge so that the integral storage rack 8 can enter and exit the cabinet 7. Then the cabinet door 72 can rotate around the hinge to facilitate opening and closing of the door; the cabinet door 72 is fixed to the cabinet body 71 through a snap fastener. A sealing strip 74 is provided on the cabinet body 71 to make the cabinet body 71 and the cabinet door 72 be hermetically connected when the cabinet door 72 is closed. After the snap fastener locks the cabinet body 71 and the cabinet door 72, the sealing strip 74 can achieve sealing, so that the vacuum degree in the cabinet 7 is maintained at the set value. Preferably, multiple reinforcing ribs 73 are installed on the inner wall of the cabinet body 71, so as to improve the strength of the cabinet body 71.

[0043] The cabinet body 71 of the cabinet 7 is connected with a negative pressure gauge to obtain the negative pressure value in the cabinet 7. The negative pressure value is also one of the factors that have a greater impact on the transfection effect, so it needs to be measured.

[0044] The integral storage rack 8 includes a support frame 81 and multiple partition boards 82. The support frame 81 is a frame structure formed by splicing rods, and multiple horizontal partition boards 82 are installed on the support frame 81 from top to bottom, thereby dividing the support frame 81 into multiple layers. The integral storage rack 8 is placed in the cabinet body 71;

[0045] Each of the partition boards 82 is respectively provided with one of the water tanks 9;

[0046] For each of the water tanks 9, the overall shape is rectangular, and it has a plurality of notches 91 which are arranged in an array.

[0047] Preferably, both the cabinet 7 and the water tank 9 are made of stainless steel materials, which are durable.

[0048] Furthermore, the bottom of the integral storage rack 8 has a space 83 for accommodating the forks of a forklift, so that the forklift can send the integral storage rack 8 into and out of the cabinet body 71. The integral storage rack 8 is relatively large in size, so it is assisted by a forklift to enter and exit the cabinet body 71. Multiple water tanks 9 can be arranged vertically at one time.

[0049] Furthermore, a drain hole and a drain valve are provided at the bottom of the cabinet body 71 of the cabinet 7, and the drain hole is connected to the drain valve. After the vacuum impregnation operation is completed, the liquid spilled from the plants can flow into the drain hole and then be discharged through the drain valve, avoiding excessive humidity inside the cabinet 7 and extending the service life of the cabinet 7.

[0050] Furthermore, the partition 82 is adjustably installed at the upper and lower positions on the integral storage rack 8, so that the space 83 between two adjacent partitions 82 can be adjusted and the size of the support frame 81 can be adapted, so that more water tanks 9 can be placed.

[0051] Furthermore, the vacuum pump is a rotary vane vacuum pump, and the vacuum pump has an oil mist filter. The rotary vane vacuum pump is mainly composed of parts such as a stator, a rotor, a rotary vane, a stator cover, and a spring. Its structure utilizes a rotor eccentrically installed in the stator cavity (the outer circle of the rotor is tangent to the inner surface of the stator, and the gap between them is very small) and two rotary vanes sliding in the rotor slots and tightly attached to the inner wall of the stator by spring tension and centrifugal force. When the rotor rotates, it always slides along the inner wall of the stator.

[0052] Refer to Figures 2 to 4 , according to another aspect of the present invention, there is also provided a vacuum impregnation device suitable for transient expression of plant vacuum impregnation, including the vacuum box described above, and further including a planting device. The planting device includes a planting pot assembly 1, a cover plate 2, and a buckle 3, wherein:

[0053] The planting pot assembly 1 includes a planting pot mounting plate 11 and a plurality of planting pots 12. The planting pot mounting plate 11 is integrally formed with all the planting pots 12. The planting pot assembly 1 can be made of ceramic materials and formed by 3D printing technology. Each planting pot 12 can plant one plant, such as Nicotiana benthamiana.

[0054] For each of the planting pots 12, it has an integrally formed pot body 121 and a bottom plate 122. The pot body 121 is preferably cylindrical. The top of the pot body 121 is fixed to the planting pot mounting plate 11. A collecting groove 123 and a plurality of circumferentially arranged grooves 124 are provided on the bottom surface of the bottom plate 122. The grooves 124 are preferably straight grooves. Each of the grooves 124 is respectively communicated with the collecting groove 123. The collecting groove 123 is a circular groove for collecting the nutrient solution in the grooves 124. More than three grooves 124 are preferably provided on the planting pot 12. Four grooves 124 are preferably provided on the planting pot 12 and they are arranged in a cross shape, so that the nutrient solution can flow into the collecting groove 123 from four directions. A plurality of first through holes 125 are further provided on the bottom plate 122, and the nutrient solution can flow into the planting pot 12 through these first through holes 125 to provide nutrients for the plants.

[0055] At least one of the first through holes 125 is provided between any two adjacent grooves 124. Then these first through holes 125 are distributed at multiple positions on the bottom of the pot. The nutrient solution can enter the planting pot 12 from multiple directions to provide nutrients for the plants. All the first through holes 125 are more preferably circumferentially distributed, and the number of the first through holes 125 between any two adjacent grooves 124 is equal. The arrangement of the first through holes 125 is relatively regular, so that the nutrient solution can enter the seedling substrate in the planting pot 12 more evenly.

[0056] The utility model preferably further includes a tray for receiving all the planting pots 12. By pouring the nutrient solution into the tray below the planting device, the nutrient solution flows through the lower grooves 124 and the collecting groove 123 and flows into the planting pot 12 from the first through holes 125. Therefore, the utility model waters the nutrient solution from below the planting device to enable the plants in the planting pot 12 to absorb the nutrient solution.

[0057] The planting pot mounting plate 11 is respectively provided with second through holes 126 at positions corresponding to each of the planting pots 12. Each of the second through holes 126 is respectively communicated with the inner cavity of one of the planting pots 12.

[0058] The cover plate 2 is covered on the planting pot mounting plate 11, and the cover plate 2 is respectively provided with third through holes 21 at positions corresponding to each of the second through holes 126. Each of the third through holes 21 is respectively communicated with one of the second through holes 126, and the aperture of the third through hole 21 is smaller than that of the second through hole 126 to prevent the seedling substrate and plants in the planting pot 12 from falling out. The cover plate 2 and the planting pot mounting plate 11 are fixedly connected together by a plurality of the buckles 3 so that the cover plate 2 and the planting pot assembly 1 can be placed on the water tank 9 together and the cover plate 2 contacts the water tank 9 (that is, the whole planting device is turned over and buckled on the water tank 9), facilitating the plant leaves coming out from the third through holes 21 of the cover plate 2 to be immersed in the bacterial liquid in the notch 91.

[0059] One notch 91 can correspond to one or more planting devices. Preferably, the number of the notches 91 of the present utility model is the same as the number of the planting devices. After the planting devices are placed upside down on the water tank 9, the planting devices are also arranged in an array.

[0060] The whole planting device can be carried as a whole. The second through holes 126 and the third through holes 21 are also the growth spaces of the plants, and the plants grow outwards from the second through holes 126 and the third through holes 21.

[0061] When vacuum infiltration treatment needs to be carried out on the plants, the water tank 9 can be first filled with bacterial liquid, the whole planting device is turned upside down, the cover plate 2 is placed on the water tank 9, and after the plants are immersed in the bacterial liquid, the integral storage rack 8 is placed in the cabinet 7. The leaf extraction time is generally the 8±1 days after the infiltration is completed, and the actual extraction time needs to be determined according to the observation of the leaf phenotype, and the leaves with successful infiltration and good phenotype are selected.

[0062] Furthermore, each of the buckles 3 is respectively hinged on the planting pot 12 through a horizontal hinge shaft 5, so that the buckle 3 can be conveniently turned over to lock and unlock the cover plate 2 and the planting pot mounting plate 11, and the buckle 3 is not easy to fall off because it is connected to the planting pot 12.

[0063] Furthermore, hinge seats 6 are arranged on the body 121 of each planting pot 12, and each hinge shaft 5 is respectively connected to the hinge seats 6 of two adjacent planting pots 12. Then the hinge seats 6 and the hinge shafts 5 connect the planting pots 12 in pairs, which can improve the overall strength of the planting device, and the hinge shafts 5 can also play the role of handles, facilitating the overall handling of the planting device.

[0064] Furthermore, the edge of the top surface of the cover plate 2 has a notch groove 22, that is, the edge of the cover plate 2 is thinner than the middle part.

[0065] The buckle 3 that fixedly connects the cover plate 2 and the planting pot mounting plate 11 extends into the notch groove 22, and the top surface of the buckle 3 is flush with the top surface of the cover plate 2. After their top surfaces are flush, the operation requirements during the transfection process can be reduced. The entire planting device can be placed upside down on the water tank 9 for translation, without worrying about the planting device bumping into the water tank 9 or being unable to move the planting device, making the translation of the planting device on the water tank 9 relatively easy.

[0066] Furthermore, the planting pot mounting plate 11 is integrally rectangular, and multiple planting pots 12 are arranged in an array, which is convenient for each plant to be aligned with the notch on the water tank 9 after flipping. Preferably, there are two rows and four columns of the planting pots 12 in the present utility model, a total of eight.

[0067] Furthermore, the center lines of the planting pot 12, the second through hole 126, and the third through hole 21 are coaxially arranged, which is convenient for seedling transplantation and plant growth.

[0068] The planting device of the present utility model can perform centralized planting, centralized seedling transplantation, centralized handling, and centralized infiltration, thereby improving production efficiency.

[0069] Refer to Figure 1 、 Figure 2 , each layer of the water tank 9 has 8 (2 rows × 4 columns) notches 91, and a total of five water tanks 9 are arranged up and down. Then, 8 planting devices can be placed on each water tank 9. The 8 planting devices are arranged in an array on the water tank 9. Then, the vacuum infiltration equipment of the present utility model can perform infiltration on 5 × 8 × 8 = 320 plants at a time, which is 160 times that of the small-scale infiltration of 2 plants in the laboratory, can ensure large-scale mass production, and realize commercial expansion of the production scale.

[0070] It is easy for those skilled in the art to understand that the above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. Vacuum chamber applicable to transient expression of plants by vacuum infiltration, characterized in that, It includes a vacuum pump, a cabinet, an integral storage rack and a water tank, wherein: The vacuum pump is connected to the cabinet for evacuating the interior of the cabinet. The cabinet has a cabinet body and a cabinet door. The cabinet door is rotatably mounted on the cabinet body through a vertical hinge to facilitate the integral storage rack to enter and exit the cabinet. The cabinet door is fixed to the cabinet body by a buckle. A sealing strip is provided on the cabinet body to make the cabinet body and the cabinet door be hermetically connected when the cabinet door is closed. The integral storage rack includes a support frame and multiple partition boards. The support frame is a frame structure formed by splicing rods, and multiple horizontal partition boards are installed on the support frame from top to bottom, thereby dividing the support frame into multiple layers. The integral storage rack is placed inside the cabinet. One water tank is placed on each partition board. For each water tank, its whole is rectangular, it has multiple notches and these notches are arranged in an array.

2. The vacuum chamber applicable to transient expression of plant vacuum infiltration according to claim 1, wherein A negative pressure gauge is connected to the cabinet body of the cabinet for obtaining the negative pressure value inside the cabinet.

3. The vacuum chamber applicable to transient expression by plant vacuum infiltration according to claim 1, wherein The bottom of the integral storage rack has a space for accommodating the forks of a forklift, so that the forklift can send the integral storage rack into and out of the cabinet body.

4. The vacuum chamber applicable to transient expression of plant vacuum infiltration according to claim 1, wherein The number of the vacuum pumps is two. Two vacuum pump interfaces are provided on the cabinet body of the cabinet, and each vacuum pump interface is respectively connected to one vacuum pump.

5. The vacuum chamber applicable to transient expression of plant vacuum infiltration according to claim 1, characterized in that The vacuum pump is a rotary vane vacuum pump, and the vacuum pump has an oil mist filter.

6. The vacuum chamber applicable to transient expression of plant vacuum infiltration according to claim 1, characterized in that, A drain hole and a drain valve are provided at the bottom of the cabinet body of the cabinet, and the drain hole is connected to the drain valve.

7. A vacuum infiltration device suitable for transient expression in plant vacuum infiltration, comprising the vacuum chamber according to any one of claims 1 to 6, characterized in that, It further includes a planting device, which includes a planting pot assembly, a cover plate and a buckle, wherein: The planting pot assembly includes a planting pot mounting plate and multiple planting pots. The planting pot mounting plate is integrally formed with all the planting pots. For each planting pot, it has an integrally formed pot body and a bottom plate. The top of its pot body is fixed to the planting pot mounting plate. A collecting groove and multiple circumferentially arranged grooves are provided on the bottom surface of its bottom plate. Each groove is respectively communicated with the collecting groove. Multiple first through holes are also provided on the bottom plate. At least one first through hole is provided between any two adjacent grooves. The planting pot mounting plate is respectively provided with second through holes at positions corresponding to each planting pot. Each second through hole is respectively communicated with the inner cavity of a planting pot. The cover plate covers the planting pot mounting plate, and the cover plate is respectively provided with third through holes at positions corresponding to each second through hole. Each third through hole is respectively communicated with a second through hole, and the aperture of the third through hole is smaller than that of the second through hole. The cover plate and the planting pot mounting plate are fixedly connected together by multiple buckles, so as to place the cover plate on the water tank to immerse the plant leaves in the bacterial liquid in the notches for infiltration.

8. The vacuum infiltration device applicable to transient expression of plant vacuum infiltration according to claim 7, characterized in that, Each buckle is respectively hinged to the planting pot through a horizontal hinge shaft.

9. The vacuum infiltration device applicable to transient expression of plant vacuum infiltration according to claim 8, characterized in that, Hinge seats are provided on the pot bodies of each planting pot, and each hinge shaft is respectively connected to the hinge seats of two adjacent planting pots.

10. The vacuum infiltration device applicable to transient expression of plant vacuum infiltration according to claim 7, characterized in that, A notch groove is provided at the edge of the top surface of the cover plate. The buckle that fixedly connects the cover plate and the planting pot mounting plate extends into the notch groove, and the top surface of the buckle is flush with the top surface of the cover plate.

Images