Mesenchymal stem cell exosome preservation device

Abstract

The utility model discloses a mesenchymal stem cell exosome preservation device which comprises a storage box, the upper surface of the storage box is fixedly connected with a pick-and-place mechanism, the pick-and-place mechanism comprises a fixing frame, the side surface of the top end of the fixing frame is fixedly connected with a first sliding seat, the side surface of the first sliding seat is fixedly connected with a first motor, and the first motor is fixedly connected with a second motor. The output end of the first motor is fixedly connected with a first lead screw, one end of the first lead screw is in threaded connection with a first threaded seat, the side surface of the first threaded seat is fixedly connected with a mounting plate, the side surface of the mounting plate is fixedly connected with a second sliding seat, and the side surface of the second sliding seat is fixedly connected with a second motor; by arranging the picking and placing mechanism, artificial contact of stem cell exosomes is reduced, damage to stem cell structures caused by bacterium breeding generated by artificial contact is reduced, the time of exposure of the picking and placing storage tube in a room temperature state is shortened, and the storage effect of the mesenchymal stem cells is enhanced.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome storage devices, and in particular to a mesenchymal stem cell exosome storage device. Background Art

[0002] Exosomes derived from mesenchymal stem cells can be transferred between cells through the proteins and other components in their contents, and play similar roles as mesenchymal stem cells in tissue repair, immunosuppression, and immunoregulation. Therefore, exosome research is expected to become a key research direction in the future biomedicine field.

[0003] Existing mesenchymal stem cell exosome preservation devices often require manual handling when placing storage tubes, and manual handling may cause bacteria to grow in the storage box, resulting in damage to the stem cell structure. In addition, manual handling can usually only be performed on a single storage tube, which is inefficient and causes all storage tubes to be exposed to room temperature, thereby reducing the storage effect of mesenchymal stem cells. For example, a Chinese patent discloses "A stem cell exosome preservation device", and its application number is "CN202322876811.5", which includes: a base, two mounting frames are symmetrically fixedly connected to the side wall on the upper side of the base, and the side walls on the opposite sides of the two mounting frames are fixedly connected to fixed shafts, and rotating cylinders are rotatably sleeved on the outside of the two fixed shafts, and the same insert is fixedly connected between the two rotating cylinders, and a preservation test tube is inserted in the insert. When placing the storage tubes, the device often requires manual handling, and manual handling may cause bacteria to grow in the storage box, resulting in damage to the stem cell structure, and its applicability is relatively limited. Utility Model Content

[0004] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art and to provide a mesenchymal stem cell exosome storage device. By providing a pick-and-place mechanism, the device reduces manual contact with stem cell exosomes, reduces bacterial growth caused by manual contact and thus damages the stem cell structure, improves the pick-and-place efficiency, reduces the time that the storage tube is exposed to room temperature during pick-and-place, and enhances the storage effect of mesenchymal stem cells.

[0005] The present utility model also provides a preservation device for mesenchymal stem cell exosomes having the above-mentioned one, including: a storage box, a pick-and-place mechanism is fixedly connected to the upper surface of the storage box, and the pick-and-place mechanism includes: a fixed frame, a first sliding seat is fixedly connected to the top side surface of the fixed frame, a first motor is fixedly connected to the side surface of the first sliding seat, an output end of the first motor is fixedly connected to a first lead screw, one end of the first lead screw is threadedly connected to a first threaded seat, a mounting plate is fixedly connected to the side surface of the first threaded seat, a second sliding seat is fixedly connected to the side surface of the mounting plate, a second motor is fixedly connected to the side surface of the second sliding seat, an output end of the second motor is fixedly connected to a second lead screw, one end of the second lead screw is threadedly connected to a second threaded seat, and a connecting plate is fixedly connected to the side surface of the second threaded seat; through the mutual cooperation among the above-mentioned parts, it is convenient to adjust the horizontal position and vertical position of the pick-and-place mechanism.

[0006] A clamping mechanism is fixedly connected to the side surface of the connecting plate, and the clamping mechanism includes: side plates, a support plate is fixedly connected to the side surface of the side plates, a clamping cylinder is fixedly connected to the lower surface of the support plate, and clamping pieces are fixedly connected to the inner side of the clamping cylinder. Through the mutual cooperation among the above-mentioned parts, it reduces the manual contact with the stem cell exosomes, reduces the bacterial growth caused by manual contact and thus avoids the destruction of the stem cell structure, improves the pick-and-place efficiency, reduces the time for the storage tube to be exposed to room temperature during pick-and-place, and enhances the storage effect of the mesenchymal stem cells.

[0007] According to the preservation device for mesenchymal stem cell exosomes of the present utility model, the connecting plate and the side plates are fixedly connected by screws, and a silica gel pad is pasted on the inner side of the clamping pieces. Through the mutual cooperation among the above-mentioned parts, the clamping pieces are not easy to damage the storage tube.

[0008] According to the preservation device for mesenchymal stem cell exosomes of the present utility model, a hinge is fixedly connected to the top side surface of the storage box, a cover plate is fixedly connected to one side of the hinge, and a handle is fixedly connected to the upper surface of the cover plate. Through the mutual cooperation among the above-mentioned parts, it is convenient to open the storage box to pick and place the storage tube.

[0009] According to the preservation device for mesenchymal stem cell exosomes of the present utility model, a condenser is fixedly connected to the inner surface of the storage box, and a first delivery pipe is fixedly connected to the output end of the condenser. Through the mutual cooperation among the above-mentioned parts, the temperature inside the storage box can be cooled.

[0010] A preservation device for mesenchymal stem cell exosomes according to the present utility model, a blower is fixedly connected to the side surface of the first delivery pipe, a fan blade is fixedly connected to the bottom surface of the blower, and a second delivery pipe is fixedly connected to the output end of the blower. Through the mutual cooperation between the above-mentioned parts, the condensed gas enters the interior of the storage box through the first delivery pipe, which can facilitate the uniform distribution of cold air inside the storage box, making the temperature at each position inside the storage box basically the same, and there will be no large temperature difference at different positions inside the storage box.

[0011] A preservation device for mesenchymal stem cell exosomes according to the present utility model, a partition is fixedly connected to the inner surface of the storage box, and a condensation pipe is laid on the upper surface of the partition. Through the mutual cooperation between the above-mentioned parts, it is convenient for the preservation of stem cell exosomes.

[0012] A preservation device for mesenchymal stem cell exosomes according to the present utility model, a support plate is laid on the upper surface of the condensation pipe, and a placement groove is provided on the upper surface of the support plate. Through the mutual cooperation between the above-mentioned parts, it is convenient for the storage tube to be placed in the placement groove.

[0013] A preservation device for mesenchymal stem cell exosomes according to the present utility model, a storage tube is arranged on the inner surface of the placement groove, and a connecting ring is fixedly connected to the upper surface of the support plate. Through the mutual cooperation between the above-mentioned parts, it is convenient to sleeved and fix the storage tube to prevent it from shaking.

[0014] Beneficial effects

[0015] Compared with the prior art, by setting the picking and placing mechanism, the present utility model reduces the manual contact with mesenchymal stem cell exosomes, reduces the bacterial growth caused by manual contact and thus prevents the destruction of the stem cell structure, improves the picking and placing efficiency, reduces the time for the storage tube to be exposed to room temperature during picking and placing, and enhances the storage effect of mesenchymal stem cells. Brief description of the drawings

[0016] The present utility model will be further described below in conjunction with the drawings and embodiments;

[0017] Figure 1 It is the overall structure diagram of the preservation device for mesenchymal stem cell exosomes of the present utility model;

[0018] Figure 2 It is the structure diagram of the horizontal adjustment mechanism of the preservation device for mesenchymal stem cell exosomes of the present utility model;

[0019] Figure 3 It is the structure diagram of the lifting adjustment mechanism of the preservation device for mesenchymal stem cell exosomes of the present utility model;

[0020] Figure 4 It is the side sectional view of the preservation device for mesenchymal stem cell exosomes of the present utility model;

[0021] Figure 5 The enlarged view of part A in the Figure 1 preservation device for mesenchymal stem cell exosomes of the present utility model.

[0022] Figure 6 The structural diagram of the condensing pipe of the preservation device for mesenchymal stem cell exosomes of the present utility model.

[0023] Legend description:

[0024] 1. Storage box; 2. Fixed frame; 3. First sliding seat; 4. First motor; 5. First lead screw; 6. First threaded seat; 7. Mounting plate; 8. Second sliding seat; 9. Second motor; 10. Second lead screw; 11. Second threaded seat; 12. Connecting plate; 13. Side plate; 14. Support plate; 15. Clamping cylinder; 16. Clamping piece; 17. Cover plate; 18. Handle; 19. Hinge; 20. Condenser; 21. First delivery pipe; 22. Fan; 23. Fan blade; 24. Second delivery pipe; 25. Partition board; 26. Condensing pipe; 27. Support plate; 28. Storage pipe; 29. Placing groove; 30. Connecting ring. Detailed implementation manners

[0025] This part will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the drawings. The role of the drawings is to supplement the description of the text part of the specification, enabling people to intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but it cannot be understood as a limitation on the protection scope of the present utility model.

[0026] Refer to Figure 1-6, an embodiment of the present utility model provides a preservation device for mesenchymal stem cell exosomes, which includes: a storage box 1, an inner surface of the storage box 1 is fixedly connected with a partition plate 25, a condensation pipe 26 is laid on an upper surface of the partition plate 25 for facilitating the preservation of stem cell exosomes, a support plate 27 is laid on an upper surface of the condensation pipe 26, a placement groove 29 is arranged on an upper surface of the support plate 27 for facilitating the storage tube 28 to be placed in the placement groove 29, a storage tube 28 is arranged on an inner surface of the placement groove 29, a connecting ring 30 is fixedly connected to an upper surface of the support plate 27 for facilitating the sheathing and fixing of the storage tube 28 to prevent shaking, a condenser 20 is fixedly connected to an inner surface of the storage box 1, an output end of the condenser 20 is fixedly connected with a first conveying pipe 21 for cooling the temperature inside the storage box 1, a blower 22 is fixedly connected to a side surface of the first conveying pipe 21, a fan blade 23 is fixedly connected to a bottom surface of the blower 22, an output end of the blower 22 is fixedly connected with a second conveying pipe 24, so that the condensed gas enters the inside of the storage box 1 through the first conveying pipe 21, which can facilitate the uniform distribution of cold air inside the storage box 1, making the temperature at each position inside the storage box 1 basically the same, and there will be no large temperature difference at different positions inside the storage box 1. A hinge 19 is fixedly connected to a top side surface of the storage box 1, a cover plate 17 is fixedly connected to one side of the hinge 19, a handle 18 is fixedly connected to an upper surface of the cover plate 17 for facilitating the opening of the storage box 1 to take and place the storage tube 28. A taking and placing mechanism is fixedly connected to an upper surface of the storage box 1. The taking and placing mechanism includes: a fixed frame 2, a first sliding seat 3 is fixedly connected to a top side surface of the fixed frame 2, a first motor 4 is fixedly connected to a side surface of the first sliding seat 3, an output end of the first motor 4 is fixedly connected with a first lead screw 5, one end of the first lead screw 5 is threadedly connected with a first threaded seat 6, an installation plate 7 is fixedly connected to a side surface of the first threaded seat 6, a second sliding seat 8 is fixedly connected to a side surface of the installation plate 7, a second motor 9 is fixedly connected to a side surface of the second sliding seat 8, an output end of the second motor 9 is fixedly connected with a second lead screw 10, one end of the second lead screw 10 is threadedly connected with a second threaded seat 11, and a connecting plate 12 is fixedly connected to a side surface of the second threaded seat 11 for facilitating the adjustment of the horizontal and vertical positions of the taking and placing mechanism. The connecting plate 12 and a side plate 13 are fixedly connected by screws, and a silica gel pad is pasted on an inner side of the clamping piece 16, so that the clamping piece 16 is not easy to damage the storage tube 28.

[0027] A clamping mechanism is fixedly connected to a side surface of the connecting plate 12. The clamping mechanism includes: a side plate 13, a support plate 14 is fixedly connected to a side surface of the side plate 13, a clamping cylinder 15 is fixedly connected to a lower surface of the support plate 14, and a clamping piece 16 is fixedly connected to an inner side of the clamping cylinder 15, which reduces the manual contact with mesenchymal stem cell exosomes, reduces the bacterial growth caused by manual contact and thus prevents the destruction of the stem cell structure, improves the taking and placing efficiency, reduces the time for the storage tube 28 to be exposed to room temperature during taking and placing, and enhances the storage effect of mesenchymal stem cells.

[0028] Working principle: When using this device to preserve the stem cell exosomes in the storage tube 28, first open the cover plate 17 on the upper surface of the storage box 1 through the handle 18 and the hinge 19, place the storage tube 28 in the placement groove 29, and fix the storage tube 28 by sleeving it with the connecting ring 30 to prevent shaking. Start the condenser 20, and transport the condensed liquid to the condensation tube 26 through the first delivery pipe 21 and the second delivery pipe 24. The condensation tube 26 can cool the temperature inside the storage box 1. Start the fan 22 to rotate the fan blade 23, so that the condensed gas enters the inside of the storage box 1 through the first delivery pipe 21, which can facilitate the uniform distribution of cold air inside the storage box 1, making the temperature at each position inside the storage box 1 basically the same, and there will be no large temperature difference at different positions inside the storage box 1, better storing the exosomes inside the storage tubes 28 at different positions. The storage effect of the storage box 1 is good, improving the practical performance of the storage box 1. When taking and placing the stem cell exosomes in the storage tube 28, start the first motor 4 to rotate the first lead screw 5, drive the first threaded seat 6 to perform threaded rotation, and drive the horizontal position adjustment of the taking and placing mechanism. Start the second motor 9 to rotate the second lead screw 10, drive the second threaded seat 11 to perform threaded rotation, and drive the lifting position adjustment of the taking and placing mechanism. Start the clamping cylinder 15 to make the clamping piece 16 take and place multiple groups of storage tubes 28, reducing the manual contact with the stem cell exosomes, reducing the bacterial growth caused by manual contact and thus preventing the destruction of the stem cell structure, and improving the taking and placing efficiency.

[0029] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the purpose of the present invention.

Claims

1. A mesenchymal stem cell exosome preservation device, characterized in that, Including: A storage box (1), on the upper surface of the storage box (1) is fixedly connected with a picking and placing mechanism, and the picking and placing mechanism includes: a fixed frame (2), on the top side surface of the fixed frame (2) is fixedly connected with a first sliding seat (3), on the side surface of the first sliding seat (3) is fixedly connected with a first motor (4), the output end of the first motor (4) is fixedly connected with a first lead screw (5), one end of the first lead screw (5) is threadedly connected with a first threaded seat (6), on the side surface of the first threaded seat (6) is fixedly connected with a mounting plate (7), on the side surface of the mounting plate (7) is fixedly connected with a second sliding seat (8), on the side surface of the second sliding seat (8) is fixedly connected with a second motor (9), the output end of the second motor (9) is fixedly connected with a second lead screw (10), one end of the second lead screw (10) is threadedly connected with a second threaded seat (11), and on the side surface of the second threaded seat (11) is fixedly connected with a connecting plate (12). On the side surface of the connecting plate (12) is fixedly connected with a clamping mechanism, and the clamping mechanism includes: a side plate (13), on the side surface of the side plate (13) is fixedly connected with a support plate (14), on the lower surface of the support plate (14) is fixedly connected with a clamping cylinder (15), and on the inner side of the clamping cylinder (15) is fixedly connected with a clamping piece (16).

2. The preservation device for mesenchymal stem cell exosomes according to claim 1, characterized in that, The connecting plate (12) and the side plate (13) are fixedly connected by screws, and a silica gel pad is pasted on the inner side of the clamping piece (16).

3. The preservation device for mesenchymal stem cell exosomes according to claim 1, wherein On the top side surface of the storage box (1) is fixedly connected with a hinge (19), on one side of the hinge (19) is fixedly connected with a cover plate (17), and on the upper surface of the cover plate (17) is fixedly connected with a handle (18).

4. The preservation device for mesenchymal stem cell exosomes according to claim 1, characterized in that, On the inner surface of the storage box (1) is fixedly connected with a condenser (20), and the output end of the condenser (20) is fixedly connected with a first delivery pipe (21).

5. A mesenchymal stem cell exosome preservation device according to claim 4, characterized in that, On the side surface of the first delivery pipe (21) is fixedly connected with a blower (22), on the bottom surface of the blower (22) is fixedly connected with a fan blade (23), and the output end of the blower (22) is fixedly connected with a second delivery pipe (24).

6. The preservation device for mesenchymal stem cell exosomes according to claim 1, characterized in that, On the inner surface of the storage box (1) is fixedly connected with a partition (25), and on the upper surface of the partition (25) is laid a condensation pipe (26).

7. A mesenchymal stem cell exosome preservation device according to claim 6, characterized in that, On the upper surface of the condensation pipe (26) is laid a support plate (27), and on the upper surface of the support plate (27) is provided with a placement groove (29).

8. A mesenchymal stem cell exosome preservation device according to claim 7, characterized in that, On the inner surface of the placement groove (29) is provided with a storage pipe (28), and on the upper surface of the support plate (27) is fixedly connected with a connecting ring (30).

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